MD07G1307200.v1.1

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001878
Physical Location & Seq
Forward (+)
36343248 .. 36344847
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1307200.v1.1.491

Sequence Viewer

Length: 612 bp
ATGTTCGATCTGTTCCATGCCTCTCTTAAGTCTAATACTAGTTCACTTGCACCAGGAATTTCACTCAAAACTCAAGAACTCTATGCGTTGGTCTTTCTTACTCGATACCTTGACTTGATCGTCAAGTATTACTCAGTGTATAACACTCTGGGGAAGCTCATATTTATCGGAACTTCTTTTGCCACGGTCTGGCACATGAGGTACCATAAAGTAGTGAAGCAAACCTACAACAAGGATCAAGATACTTTCAGACATTACTATCTCATACTTTTATCCTTGGGGCTGGCTCTTCTGATTCCGCGCGCTTTTACTGCAATTGATGTTCTGTGGGCATTTTCAATATATCTTGAAGCTGTGGCAATCTTACCACAGTTGGTATTACTGCAGAGGTCAAGAAACATTGACAACTTAACTGGAAATTACGTTTTCCTTCTCGGTGCTTATCGTTCTTTATATCTTGTCAACTGGATTTACCGTTTCTTCACAGACCTCCACAGAGTTCGCTGGATACCTTGGATTTCAGGCCTAGTTCAGACTGCTCTTTATGCTGATTTTTTCTACTACTATATCAAGAGCTGGAAGGAACACGAGGAGCTTAAGCTTCCCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05110 map05110
Pfam Domains
Protein Families
ER_lumen_recept

Protein Analysis

204

Amino Acids

23.99

Weight (kDa)

9.43

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 18 - 159 7e-54 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
Acc65I GGTACC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 201
AccB7I CCANNNNNTGG 1 cut(s) 189
AccII CGCG 2 cut(s) 301, 303
AciI CCGC 2 cut(s) 299, 606
AclWI GGATC 1 cut(s) 243
AcsI RAATTY 1 cut(s) 57
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 189
AflII CTTAAG 2 cut(s) 26, 596
AgsI TTSAA 2 cut(s) 339, 350
AhlI ACTAGT 1 cut(s) 38
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 5 cut(s) 157, 353, 576, 595, 601
AluI AGCT 5 cut(s) 157, 353, 576, 595, 601
AlwI GGATC 1 cut(s) 243
AoxI GGCC 1 cut(s) 523
ApoI RAATTY 1 cut(s) 57
Asp718I GGTACC 1 cut(s) 201
AspLEI GCGC 2 cut(s) 303, 305
BanI GGYRCC 1 cut(s) 201
BarI GAAGNNNNNNTAC 2 cut(s) 209, 241
BauI CACGAG 1 cut(s) 587
BciT130I CCWGG 1 cut(s) 54
BciVI GTATCC 1 cut(s) 501
BcuI ACTAGT 1 cut(s) 38
BfaI CTAG 2 cut(s) 39, 527
BfmI CTRYAG 1 cut(s) 383
BfrI CTTAAG 2 cut(s) 26, 596
BfuI GTATCC 1 cut(s) 501
Bme1390I CCNGG 1 cut(s) 54
BmiI GGNNCC 1 cut(s) 203
BmrFI CCNGG 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 57
BsaJI CCNNGG 3 cut(s) 183, 276, 512
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse1I ACTGG 2 cut(s) 418, 470
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 3 cut(s) 183, 276, 512
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 2 cut(s) 418, 470
BsePI GCGCGC 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 605
Bsh1236I CGCG 2 cut(s) 301, 303
BshFI GGCC 1 cut(s) 525
BshNI GGYRCC 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 189
BsnI GGCC 1 cut(s) 525
Bsp143I GATC 3 cut(s) 7, 117, 235
BspACI CCGC 2 cut(s) 299, 606
BspANI GGCC 1 cut(s) 525
BspCNI CTCAG 1 cut(s) 146
BspFNI CGCG 2 cut(s) 301, 303
BspLI GGNNCC 1 cut(s) 203
BspMAI CTGCAG 1 cut(s) 387
BspPI GGATC 1 cut(s) 243
BspQI GCTCTTC 1 cut(s) 294
BspT107I GGYRCC 1 cut(s) 201
BspTI CTTAAG 2 cut(s) 26, 596
BsrI ACTGG 2 cut(s) 418, 470
BssECI CCNNGG 3 cut(s) 183, 276, 512
BssHII GCGCGC 1 cut(s) 301
BssMI GATC 3 cut(s) 7, 117, 235
BssSI CACGAG 1 cut(s) 587
BssT1I CCWWGG 2 cut(s) 276, 512
Bst2BI CACGAG 1 cut(s) 587
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 3 cut(s) 187, 372, 476
Bst6I CTCTTC 1 cut(s) 294
BstAFI CTTAAG 2 cut(s) 26, 596
BstC8I GCNNGC 2 cut(s) 285, 303
BstDEI CTNAG 1 cut(s) 133
BstDSI CCRYGG 1 cut(s) 183
BstFNI CGCG 2 cut(s) 301, 303
BstHHI GCGC 2 cut(s) 303, 305
BstKTI GATC 3 cut(s) 10, 120, 238
BstMBI GATC 3 cut(s) 7, 117, 235
BstMWI GCNNNNNNNGC 2 cut(s) 311, 545
BstNI CCWGG 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 52
BstSFI CTRYAG 1 cut(s) 383
BstUI CGCG 2 cut(s) 301, 303
BsuI GTATCC 1 cut(s) 501
BsuRI GGCC 1 cut(s) 525
BtgI CCRYGG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 141
Cac8I GCNNGC 2 cut(s) 285, 303
CfoI GCGC 2 cut(s) 303, 305
Csp6I GTAC 1 cut(s) 202
CviAII CATG 2 cut(s) 17, 196
CviJI RGCY 8 cut(s) 157, 283, 287, 353, 525, 576, 595, 601
CviKI_1 RGCY 8 cut(s) 157, 283, 287, 353, 525, 576, 595, 601
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 1 cut(s) 133
DpnI GATC 3 cut(s) 9, 119, 237
DpnII GATC 3 cut(s) 7, 117, 235
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
Eco130I CCWWGG 2 cut(s) 276, 512
Eco147I AGGCCT 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 52
EcoT14I CCWWGG 2 cut(s) 276, 512
ErhI CCWWGG 2 cut(s) 276, 512
FaeI CATG 2 cut(s) 20, 199
FatI CATG 2 cut(s) 16, 195
FspBI CTAG 2 cut(s) 39, 527
GlaI GCGC 2 cut(s) 302, 304
HaeIII GGCC 1 cut(s) 525
HhaI GCGC 2 cut(s) 303, 305
Hin1II CATG 2 cut(s) 20, 199
Hin6I GCGC 2 cut(s) 301, 303
HinP1I GCGC 2 cut(s) 301, 303
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 1 cut(s) 295
Hpy166II GTNNAC 2 cut(s) 44, 463
Hpy188I TCNGA 4 cut(s) 170, 251, 294, 534
Hpy188III TCNNGA 5 cut(s) 74, 239, 347, 393, 571
Hpy8I GTNNAC 2 cut(s) 44, 463
HpyAV CCTTC 2 cut(s) 440, 574
HpyCH4III ACNGT 3 cut(s) 187, 372, 476
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 3 cut(s) 50, 314, 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 545
HpyF3I CTNAG 1 cut(s) 133
HpySE526I ACGT 1 cut(s) 423
Hsp92II CATG 2 cut(s) 20, 199
HspAI GCGC 2 cut(s) 301, 303
KpnI GGTACC 1 cut(s) 205
Kzo9I GATC 3 cut(s) 7, 117, 235
LguI GCTCTTC 1 cut(s) 294
LmnI GCTCC 1 cut(s) 592
MaeI CTAG 2 cut(s) 39, 527
MaeII ACGT 1 cut(s) 423
MalI GATC 3 cut(s) 9, 119, 237
MboI GATC 3 cut(s) 7, 117, 235
MboII GAAGA 2 cut(s) 281, 472
MfeI CAATTG 1 cut(s) 315
MluCI AATT 3 cut(s) 57, 315, 418
MnlI CCTC 5 cut(s) 31, 192, 381, 500, 583
MseI TTAA 3 cut(s) 27, 410, 597
MspCI CTTAAG 2 cut(s) 26, 596
MspR9I CCNGG 1 cut(s) 54
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 54
MvnI CGCG 2 cut(s) 301, 303
MwoI GCNNNNNNNGC 2 cut(s) 311, 545
NdeII GATC 3 cut(s) 7, 117, 235
NlaIII CATG 2 cut(s) 20, 199
NlaIV GGNNCC 1 cut(s) 203
PauI GCGCGC 1 cut(s) 301
PceI AGGCCT 1 cut(s) 525
PciSI GCTCTTC 1 cut(s) 294
PfeI GAWTC 1 cut(s) 295
PflMI CCANNNNNTGG 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 203
PstI CTGCAG 1 cut(s) 387
PteI GCGCGC 1 cut(s) 301
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SapI GCTCTTC 1 cut(s) 294
SaqAI TTAA 3 cut(s) 27, 410, 597
Sau3AI GATC 3 cut(s) 7, 117, 235
ScrFI CCNGG 1 cut(s) 54
SfcI CTRYAG 1 cut(s) 383
SmlI CTYRAG 3 cut(s) 26, 72, 596
SmoI CTYRAG 3 cut(s) 26, 72, 596
SpeI ACTAGT 1 cut(s) 38
Sse9I AATT 3 cut(s) 57, 315, 418
SseBI AGGCCT 1 cut(s) 525
SsiI CCGC 2 cut(s) 299, 606
SspMI CTAG 2 cut(s) 39, 527
StuI AGGCCT 1 cut(s) 525
StyD4I CCNGG 1 cut(s) 52
StyI CCWWGG 2 cut(s) 276, 512
TaaI ACNGT 3 cut(s) 187, 372, 476
TaiI ACGT 1 cut(s) 426
TaqI TCGA 2 cut(s) 6, 103
TasI AATT 3 cut(s) 57, 315, 418
TfiI GAWTC 1 cut(s) 295
Tru1I TTAA 3 cut(s) 27, 410, 597
Tru9I TTAA 3 cut(s) 27, 410, 597
TscAI CASTG 1 cut(s) 141
TspRI CASTG 1 cut(s) 141
Van91I CCANNNNNTGG 1 cut(s) 189
Vha464I CTTAAG 2 cut(s) 26, 596
XapI RAATTY 1 cut(s) 57
XspI CTAG 2 cut(s) 39, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.