Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Reverse (-)

22550591 .. 22553677

Gene Structure 3087 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g18690.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGGGCGTCCCCGGTGGTAAGAAGAGGATCACTATCATTGCGGTCTCGTCGGTGCTCCTGGTGGCCATGGTTATCTGTGGGGTGGTTGGTGTTATCCAATACAAAAAACAAGAGCAACGCGGAGGTGGAGGCGGAGGCGGAGATGAAGACGGAGACACTAACTCCGGAGATAAAAATGCCTCCCATACATCAAAGACAATTGTAGCCATTTGTGAGCCCACCAACTACAAACAAGAATGTGAGAAGAGTCTGGAAAATGAGGCCACCGGAGACAAGAAAGACCCGCAAGACCTTGCAAGAGTCGGGATTCAGGTGGCCATAAGCAAACTTGATGAGGTTTTAAAGAACTCCACACTCTTGAAAAATAAAGGCAAAGATCCCATGACCCAAGAAGCTCTCAGTATCTGCAGGCAGTTGTTGAATGCTTCTATGTATGATTTGAAAATTTCTTACAATAAAATGTGTGCATTAAATATGGAAAAGGTTGATCAATACGCAGAAGATGTGAGGGTTTGGTTGAGTGGGGCTGTTAATCACCAAGAGACTTGCGTTGATGTATGTCAGAACACAACCGCTGATCTCGGCAAGCAAATGAATGAAACTTTGGAGTTACCTCAAGAACGCGCTAGCAATGCACTTGCCATGGTCAGCGAACTCACTGCCGCCTTGAACTCTCTTAATTTCCAGGCCAGCAGACGACGGCTCCTGGCCTCATCGCCCCCATCCAAGAACAAGAATAATGATCAACATATGCAGCAAGGCAGCCGAACCCAACAGATCCCTGCTTGGCTCACTGGTCGTAAGTTGGATCTTCTGAAAGCCACGCCAGAGACCCTTAAGCCGAACGTGACGGTGGCCCAAGATGGGAGCGGTAAGTACAAAACCATCAACGAAGCTCTGAAGCTGGTCCCGGAGAAGAGCGACGACTTGTTTGTGATTTACGTGAAGGAGGGAGTGTACAAAGAATATGTGACGATTGAAGGTATGATGGAAAATGTTGTGATTATTGGTGATGGCCCTGCTAAGACCAAGATCTCTGGTAATAAAAACTCCACTAGCGGAACCAAAACAATTAACACTGCAACATTTGCTGTAGTTGGAACATTTTTTGTTGCCAAGGACATAGGGTTTGAGAACACAGCCGGAGCTGAAGGGCACCAAGCGGTGGCGCTGCGTGTTCAATCGGATTTCTCCATCTTCTACAATTGCCGCATTGACGGGTACCAAAACACCCTATACGTCCAAGCCTACAGGAATTACTTCCGGGACTGCAACATCACTGGCGCAGTTGACTTCATCTTCGGCGACGCAGTCTCTTTCTTCCAGAACTGCAGTATGCTGGTGAGGAAGCCAAAGGACGGCCAGGCCGCCGTGGTCACAGCCCAAGGAAGGACAGACAAGAATGAGGTCACCGGGATCGTGCTCCATAACTGCACCATCAGCGGCGACCCGGAGAACAAGGATAACAAGTACAAGGCATACCTAGGTCGACCATTGAAGAGCTTCGCAAGAACCATCGTCATGCAGTCACAGATCGGTGATGTGATTCAGCCTGAAGGGTACCTGCCTACGGATGGCGAAAGGTTCCACATGACGTCATTATTCGGAGAGTACAACAATACGGGATCTGGTGCTGACATGTCCGGCAGAGCCAAATGGCCTAGCATCAAGAAGCTTGATGCTAAACAAGCTAAAGCCTACAGCGGTGGAAAGTGGTCTGAGGGTGATAGGTGGGTTAAGCCCAGCGGAGTCCCATTTGTTTCTGACTCTCTTCTTGCACCGTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

596

Amino Acids

65.0

Weight (kDa)

8.29

Isoelectric Point (pI)

29.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	66 - 215	7e-22	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	282 - 575	2.5e-118	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1598
Acc36I	ACCTGC	1 cut(s)	1572
Acc65I	GGTACC	2 cut(s)	1221, 1560
AccB1I	GGYRCC	3 cut(s)	1155, 1221, 1560
AccB7I	CCANNNNNTGG	1 cut(s)	1165
AccBSI	CCGCTC	1 cut(s)	870
AccI	GTMKAC	1 cut(s)	1489
AccII	CGCG	2 cut(s)	120, 624
AccIII	TCCGGA	1 cut(s)	164
AclWI	GGATC	6 cut(s)	35, 371, 772, 816, 1424, 1633
AcoI	YGGCCR	3 cut(s)	63, 315, 1360
AcsI	RAATTY	1 cut(s)	444
AcuI	CTGAAG	3 cut(s)	920, 1170, 1575
AcyI	GRCGYC	2 cut(s)	6, 1595
AfaI	GTAC	6 cut(s)	878, 959, 1223, 1472, 1562, 1613
AfiI	CCNNNNNNNGG	6 cut(s)	864, 1165, 1358, 1389, 1570, 1574
AflII	CTTAAG	1 cut(s)	836
AflIII	ACRYGT	1 cut(s)	1638
AgsI	TTSAA	7 cut(s)	361, 421, 442, 670, 980, 1181, 1498
AjnI	CCWGG	4 cut(s)	57, 684, 705, 1362
AjuI	GAANNNNNNNTTGG	2 cut(s)	587, 619
AluBI	AGCT	7 cut(s)	395, 896, 904, 1148, 1503, 1675, 1691
AluI	AGCT	7 cut(s)	395, 896, 904, 1148, 1503, 1675, 1691
Alw21I	GWGCWC	2 cut(s)	57, 1425
Alw26I	GTCTC	6 cut(s)	49, 147, 264, 536, 824, 1318
AlwI	GGATC	6 cut(s)	35, 371, 772, 816, 1424, 1633
AlwNI	CAGNNNCTG	1 cut(s)	405
Aor13HI	TCCGGA	1 cut(s)	164
ApeKI	GCWGC	3 cut(s)	754, 762, 1171
ApoI	RAATTY	1 cut(s)	444
ArsI	GACNNNNNNTTYG	6 cut(s)	914, 946, 1112, 1144, 1283, 1315
Asp700I	GAANNNNTTC	2 cut(s)	1259, 1502
Asp718I	GGTACC	2 cut(s)	1221, 1560
AspA2I	CCTAGG	1 cut(s)	1483
AspLEI	GCGC	3 cut(s)	626, 1171, 1286
AspS9I	GGNCC	3 cut(s)	856, 907, 1016
AsuC2I	CCSGG	5 cut(s)	12, 911, 1265, 1414, 1451
AsuHPI	GGTGA	6 cut(s)	527, 1022, 1354, 1402, 1550, 1736
AsuNHI	GCTAGC	1 cut(s)	626
AvaII	GGWCC	1 cut(s)	907
AvrII	CCTAGG	1 cut(s)	1483
BaeGI	GKGCMC	1 cut(s)	1158
BalI	TGGCCA	2 cut(s)	65, 317
BanI	GGYRCC	3 cut(s)	1155, 1221, 1560
BanII	GRGCYC	1 cut(s)	219
BbsI	GAAGAC	1 cut(s)	153
Bbv12I	GWGCWC	2 cut(s)	57, 1425
BbvI	GCAGC	3 cut(s)	766, 774, 1158
BccI	CCATC	9 cut(s)	730, 857, 893, 982, 1007, 1202, 1445, 1523, 1568
BceAI	ACGGC	3 cut(s)	716, 1355, 1375
BcgI	CGANNNNNNTGC	2 cut(s)	641, 675
BciT130I	CCWGG	4 cut(s)	59, 686, 707, 1364
BclI	TGATCA	2 cut(s)	487, 742
BcnI	CCSGG	5 cut(s)	12, 911, 1265, 1414, 1451
BcoDI	GTCTC	6 cut(s)	49, 147, 264, 536, 824, 1318
BfaI	CTAG	4 cut(s)	627, 1056, 1484, 1662
BfmI	CTRYAG	5 cut(s)	406, 1092, 1249, 1330, 1699
BfoI	RGCGCY	1 cut(s)	1172
BfrI	CTTAAG	1 cut(s)	836
BfuAI	ACCTGC	1 cut(s)	1572
BglII	AGATCT	1 cut(s)	1032
BisI	GCNGC	7 cut(s)	663, 755, 763, 1172, 1210, 1368, 1444
BlnI	CCTAGG	1 cut(s)	1483
BlsI	GCNGC	7 cut(s)	664, 756, 764, 1173, 1211, 1369, 1445
Bme1390I	CCNGG	9 cut(s)	12, 59, 686, 707, 911, 1265, 1364, 1414, 1451
Bme18I	GGWCC	1 cut(s)	907
BmgT120I	GGNCC	3 cut(s)	856, 907, 1016
BmiI	GGNNCC	7 cut(s)	704, 909, 1063, 1157, 1223, 1562, 1586
BmrFI	CCNGG	9 cut(s)	12, 59, 686, 707, 911, 1265, 1364, 1414, 1451
BmsI	GCATC	2 cut(s)	1669, 1674
BmtI	GCTAGC	1 cut(s)	630
BpiI	GAAGAC	1 cut(s)	153
BpuEI	CTTGAG	1 cut(s)	600
BpuMI	CCSGG	5 cut(s)	12, 911, 1265, 1414, 1451
BsaAI	YACGTR	1 cut(s)	943
BsaBI	GATNNNNATC	1 cut(s)	32
BsaHI	GRCGYC	2 cut(s)	6, 1595
BsaI	GGTCTC	2 cut(s)	49, 824
BsaJI	CCNNGG	8 cut(s)	10, 66, 642, 1116, 1371, 1384, 1483, 1781
BsaWI	WCCGGW	2 cut(s)	164, 266
BsaXI	ACNNNNNCTCC	6 cut(s)	146, 176, 687, 717, 941, 971
Bsc4I	CCNNNNNNNGG	6 cut(s)	864, 1165, 1358, 1389, 1570, 1574
Bse1I	ACTGG	2 cut(s)	799, 1285
Bse3DI	GCAATG	2 cut(s)	36, 637
Bse8I	GATNNNNATC	1 cut(s)	32
BseAI	TCCGGA	1 cut(s)	164
BseBI	CCWGG	4 cut(s)	59, 686, 707, 1364
BseDI	CCNNGG	8 cut(s)	10, 66, 642, 1116, 1371, 1384, 1483, 1781
BseGI	GGATG	2 cut(s)	722, 1579
BseJI	GATNNNNATC	1 cut(s)	32
BseLI	CCNNNNNNNGG	6 cut(s)	864, 1165, 1358, 1389, 1570, 1574
BseMI	GCAATG	2 cut(s)	36, 637
BseMII	CTCAG	2 cut(s)	412, 1710
BseNI	ACTGG	2 cut(s)	799, 1285
BseSI	GKGCMC	1 cut(s)	1158
BseXI	GCAGC	3 cut(s)	766, 774, 1158
BseYI	CCCAGC	1 cut(s)	1742
BsgI	GTGCAG	1 cut(s)	1417
Bsh1236I	CGCG	2 cut(s)	120, 624
BshNI	GGYRCC	3 cut(s)	1155, 1221, 1560
BsiHKAI	GWGCWC	2 cut(s)	57, 1425
BsiSI	CCGG	9 cut(s)	12, 165, 267, 911, 1143, 1264, 1413, 1451, 1644
BslFI	GGGAC	3 cut(s)	893, 1280, 1736
BslI	CCNNNNNNNGG	6 cut(s)	864, 1165, 1358, 1389, 1570, 1574
BsmAI	GTCTC	6 cut(s)	49, 147, 264, 536, 824, 1318
BsmFI	GGGAC	3 cut(s)	893, 1280, 1736
BsmI	GAATGC	1 cut(s)	427
Bso31I	GGTCTC	2 cut(s)	49, 824
Bsp1286I	GDGCHC	4 cut(s)	57, 219, 1158, 1425
Bsp13I	TCCGGA	1 cut(s)	164
Bsp1407I	TGTACA	1 cut(s)	957
Bsp19I	CCATGG	2 cut(s)	66, 642
BspCNI	CTCAG	2 cut(s)	411, 1711
BspEI	TCCGGA	1 cut(s)	164
BspFNI	CGCG	2 cut(s)	120, 624
BspLI	GGNNCC	7 cut(s)	704, 909, 1063, 1157, 1223, 1562, 1586
BspMAI	CTGCAG	2 cut(s)	410, 1334
BspMI	ACCTGC	1 cut(s)	1572
BspOI	GCTAGC	1 cut(s)	630
BspPI	GGATC	6 cut(s)	35, 371, 772, 816, 1424, 1633
BspQI	GCTCTTC	2 cut(s)	911, 1493
BspT107I	GGYRCC	3 cut(s)	1155, 1221, 1560
BspTI	CTTAAG	1 cut(s)	836
BspTNI	GGTCTC	2 cut(s)	49, 824
BsrBI	CCGCTC	1 cut(s)	870
BsrDI	GCAATG	2 cut(s)	36, 637
BsrGI	TGTACA	1 cut(s)	957
BsrI	ACTGG	2 cut(s)	799, 1285
BssECI	CCNNGG	8 cut(s)	10, 66, 642, 1116, 1371, 1384, 1483, 1781
BssNI	GRCGYC	2 cut(s)	6, 1595
BssT1I	CCWWGG	5 cut(s)	66, 642, 1116, 1384, 1483
Bst2UI	CCWGG	4 cut(s)	59, 686, 707, 1364
Bst4CI	ACNGT	2 cut(s)	853, 1782
Bst6I	CTCTTC	5 cut(s)	17, 239, 911, 1493, 1776
BstACI	GRCGYC	2 cut(s)	6, 1595
BstAFI	CTTAAG	1 cut(s)	836
BstAPI	GCANNNNNTGC	1 cut(s)	1088
BstAUI	TGTACA	1 cut(s)	957
BstBAI	YACGTR	1 cut(s)	943
BstC8I	GCNNGC	4 cut(s)	410, 587, 628, 691
BstDEI	CTNAG	3 cut(s)	398, 1023, 1719
BstDSI	CCRYGG	4 cut(s)	66, 642, 1371, 1781
BstEII	GGTNACC	1 cut(s)	1408
BstF5I	GGATG	2 cut(s)	722, 1579
BstFNI	CGCG	2 cut(s)	120, 624
BstH2I	RGCGCY	1 cut(s)	1172
BstHHI	GCGC	3 cut(s)	626, 1171, 1286
BstMAI	GTCTC	6 cut(s)	49, 147, 264, 536, 824, 1318
BstMWI	GCNNNNNNNGC	4 cut(s)	632, 1088, 1440, 1688
BstNI	CCWGG	4 cut(s)	59, 686, 707, 1364
BstNSI	RCATGY	1 cut(s)	1642
BstPI	GGTNACC	1 cut(s)	1408
BstSCI	CCNGG	9 cut(s)	10, 57, 684, 705, 909, 1263, 1362, 1412, 1449
BstSFI	CTRYAG	5 cut(s)	406, 1092, 1249, 1330, 1699
BstSLI	GKGCMC	1 cut(s)	1158
BstUI	CGCG	2 cut(s)	120, 624
BstV1I	GCAGC	3 cut(s)	766, 774, 1158
BstV2I	GAAGAC	1 cut(s)	153
BstX2I	RGATCY	5 cut(s)	376, 777, 808, 1032, 1625
BstYI	RGATCY	5 cut(s)	376, 777, 808, 1032, 1625
BtgI	CCRYGG	4 cut(s)	66, 642, 1371, 1781
BtgZI	GCGATG	1 cut(s)	699
BtsCI	GGATG	2 cut(s)	722, 1579
BtsI	GCAGTG	2 cut(s)	657, 1077
BtsIMutI	CAGTG	4 cut(s)	657, 792, 1077, 1278
BveI	ACCTGC	1 cut(s)	1572
Cac8I	GCNNGC	4 cut(s)	410, 587, 628, 691
CaiI	CAGNNNCTG	1 cut(s)	405
CfoI	GCGC	3 cut(s)	626, 1171, 1286
Cfr13I	GGNCC	3 cut(s)	856, 907, 1016
CseI	GACGC	1 cut(s)	1316
Csp6I	GTAC	6 cut(s)	877, 958, 1222, 1471, 1561, 1612
CviAII	CATG	6 cut(s)	67, 382, 643, 1522, 1591, 1639
CviQI	GTAC	6 cut(s)	877, 958, 1222, 1471, 1561, 1612
DdeI	CTNAG	3 cut(s)	398, 1023, 1719
DraI	TTTAAA	1 cut(s)	342
EaeI	YGGCCR	3 cut(s)	63, 315, 1360
Eam1104I	CTCTTC	5 cut(s)	17, 239, 911, 1493, 1776
EarI	CTCTTC	5 cut(s)	17, 239, 911, 1493, 1776
EciI	GGCGGA	2 cut(s)	147, 153
Eco130I	CCWWGG	5 cut(s)	66, 642, 1116, 1384, 1483
Eco24I	GRGCYC	1 cut(s)	219
Eco31I	GGTCTC	2 cut(s)	49, 824
Eco47I	GGWCC	1 cut(s)	907
Eco57I	CTGAAG	3 cut(s)	920, 1170, 1575
Eco91I	GGTNACC	1 cut(s)	1408
EcoO65I	GGTNACC	1 cut(s)	1408
EcoRII	CCWGG	4 cut(s)	57, 684, 705, 1362
EcoT14I	CCWWGG	5 cut(s)	66, 642, 1116, 1384, 1483
EcoT38I	GRGCYC	1 cut(s)	219
ErhI	CCWWGG	5 cut(s)	66, 642, 1116, 1384, 1483
FaeI	CATG	6 cut(s)	70, 385, 646, 1525, 1594, 1642
FaqI	GGGAC	3 cut(s)	893, 1280, 1736
FatI	CATG	6 cut(s)	66, 381, 642, 1521, 1590, 1638
FauI	CCCGC	1 cut(s)	291
FauNDI	CATATG	1 cut(s)	750
FbaI	TGATCA	2 cut(s)	487, 742
FblI	GTMKAC	1 cut(s)	1489
Fnu4HI	GCNGC	7 cut(s)	663, 755, 763, 1172, 1210, 1368, 1444
FokI	GGATG	2 cut(s)	709, 1586
FriOI	GRGCYC	1 cut(s)	219
Fsp4HI	GCNGC	7 cut(s)	663, 755, 763, 1172, 1210, 1368, 1444
FspBI	CTAG	4 cut(s)	627, 1056, 1484, 1662
GlaI	GCGC	3 cut(s)	625, 1170, 1285
GluI	GCNGC	7 cut(s)	663, 755, 763, 1172, 1210, 1368, 1444
GsaI	CCCAGC	1 cut(s)	1746
HaeII	RGCGCY	1 cut(s)	1172
HapII	CCGG	9 cut(s)	12, 165, 267, 911, 1143, 1264, 1413, 1451, 1644
HgaI	GACGC	1 cut(s)	1316
HhaI	GCGC	3 cut(s)	626, 1171, 1286
Hin1I	GRCGYC	2 cut(s)	6, 1595
Hin1II	CATG	6 cut(s)	70, 385, 646, 1525, 1594, 1642
Hin6I	GCGC	3 cut(s)	624, 1169, 1284
HinP1I	GCGC	3 cut(s)	624, 1169, 1284
HincII	GTYRAC	2 cut(s)	1291, 1490
HindII	GTYRAC	2 cut(s)	1291, 1490
HindIII	AAGCTT	1 cut(s)	1673
HinfI	GANTC	6 cut(s)	247, 300, 307, 1546, 1749, 1766
HpaII	CCGG	9 cut(s)	12, 165, 267, 911, 1143, 1264, 1413, 1451, 1644
HphI	GGTGA	6 cut(s)	527, 1022, 1354, 1402, 1550, 1736
Hpy166II	GTNNAC	3 cut(s)	958, 1291, 1490
Hpy188I	TCNGA	7 cut(s)	564, 816, 900, 1186, 1607, 1720, 1765
Hpy188III	TCNNGA	7 cut(s)	165, 251, 304, 358, 617, 1324, 1669
Hpy8I	GTNNAC	3 cut(s)	958, 1291, 1490
Hpy99I	CGWCG	4 cut(s)	52, 702, 926, 1310
HpyAV	CCTTC	5 cut(s)	940, 974, 1145, 1383, 1550
HpyCH4III	ACNGT	2 cut(s)	853, 1782
HpyCH4IV	ACGT	4 cut(s)	846, 942, 1239, 1595
HpyF10VI	GCNNNNNNNGC	4 cut(s)	632, 1088, 1440, 1688
HpyF3I	CTNAG	3 cut(s)	398, 1023, 1719
HpySE526I	ACGT	4 cut(s)	846, 942, 1239, 1595
Hsp92I	GRCGYC	2 cut(s)	6, 1595
Hsp92II	CATG	6 cut(s)	70, 385, 646, 1525, 1594, 1642
HspAI	GCGC	3 cut(s)	624, 1169, 1284
Kpn2I	TCCGGA	1 cut(s)	164
KpnI	GGTACC	2 cut(s)	1225, 1564
Ksp22I	TGATCA	2 cut(s)	487, 742
LguI	GCTCTTC	2 cut(s)	911, 1493
LmnI	GCTCC	5 cut(s)	60, 708, 867, 1145, 1428
Lsp1109I	GCAGC	3 cut(s)	766, 774, 1158
LweI	GCATC	2 cut(s)	1669, 1674
MaeI	CTAG	4 cut(s)	627, 1056, 1484, 1662
MaeII	ACGT	4 cut(s)	846, 942, 1239, 1595
MaeIII	GTNAC	6 cut(s)	609, 847, 970, 1375, 1408, 1527
MbiI	CCGCTC	1 cut(s)	870
MfeI	CAATTG	2 cut(s)	198, 1204
MflI	RGATCY	5 cut(s)	376, 777, 808, 1032, 1625
MhlI	GDGCHC	4 cut(s)	57, 219, 1158, 1425
MlsI	TGGCCA	2 cut(s)	65, 317
MluCI	AATT	6 cut(s)	198, 444, 679, 1071, 1204, 1255
MluNI	TGGCCA	2 cut(s)	65, 317
MlyI	GAGTC	4 cut(s)	256, 309, 1758, 1760
MmeI	TCCRAC	2 cut(s)	786, 1078
Mox20I	TGGCCA	2 cut(s)	65, 317
MroI	TCCGGA	1 cut(s)	164
MroXI	GAANNNNTTC	2 cut(s)	1259, 1502
MscI	TGGCCA	2 cut(s)	65, 317
MseI	TTAA	7 cut(s)	341, 470, 531, 678, 837, 1074, 1737
MslI	CAYNNNNRTG	1 cut(s)	1520
Msp20I	TGGCCA	2 cut(s)	65, 317
MspA1I	CMGCKG	4 cut(s)	575, 1443, 1704, 1746
MspCI	CTTAAG	1 cut(s)	836
MspI	CCGG	9 cut(s)	12, 165, 267, 911, 1143, 1264, 1413, 1451, 1644
MspR9I	CCNGG	9 cut(s)	12, 59, 686, 707, 911, 1265, 1364, 1414, 1451
MunI	CAATTG	2 cut(s)	198, 1204
Mva1269I	GAATGC	1 cut(s)	427
MvaI	CCWGG	4 cut(s)	59, 686, 707, 1364
MvnI	CGCG	2 cut(s)	120, 624
MwoI	GCNNNNNNNGC	4 cut(s)	632, 1088, 1440, 1688
NciI	CCSGG	5 cut(s)	12, 911, 1265, 1414, 1451
NcoI	CCATGG	2 cut(s)	66, 642
NdeI	CATATG	1 cut(s)	750
NheI	GCTAGC	1 cut(s)	626
NlaIII	CATG	6 cut(s)	70, 385, 646, 1525, 1594, 1642
NlaIV	GGNNCC	7 cut(s)	704, 909, 1063, 1157, 1223, 1562, 1586
NmeAIII	GCCGAG	1 cut(s)	561
NmuCI	GTSAC	5 cut(s)	847, 970, 1375, 1408, 1527
NspI	RCATGY	1 cut(s)	1642
PciI	ACATGT	1 cut(s)	1638
PciSI	GCTCTTC	2 cut(s)	911, 1493
PctI	GAATGC	1 cut(s)	427
PdmI	GAANNNNTTC	2 cut(s)	1259, 1502
PfeI	GAWTC	2 cut(s)	307, 1546
PflFI	GACNNNGTC	1 cut(s)	1310
PflMI	CCANNNNNTGG	1 cut(s)	1165
PfoI	TCCNGGA	2 cut(s)	909, 1263
PkrI	GCNGC	7 cut(s)	664, 756, 764, 1173, 1211, 1369, 1445
PleI	GAGTC	4 cut(s)	255, 308, 1757, 1760
PpsI	GAGTC	4 cut(s)	255, 308, 1757, 1760
Ppu21I	YACGTR	1 cut(s)	943
PscI	ACATGT	1 cut(s)	1638
Psp6I	CCWGG	4 cut(s)	57, 684, 705, 1362
PspEI	GGTNACC	1 cut(s)	1408
PspFI	CCCAGC	1 cut(s)	1742
PspGI	CCWGG	4 cut(s)	57, 684, 705, 1362
PspN4I	GGNNCC	7 cut(s)	704, 909, 1063, 1157, 1223, 1562, 1586
PspPI	GGNCC	3 cut(s)	856, 907, 1016
PstI	CTGCAG	2 cut(s)	410, 1334
PstNI	CAGNNNCTG	1 cut(s)	405
PsuI	RGATCY	5 cut(s)	376, 777, 808, 1032, 1625
PsyI	GACNNNGTC	1 cut(s)	1310
RsaI	GTAC	6 cut(s)	878, 959, 1223, 1472, 1562, 1613
RsaNI	GTAC	6 cut(s)	877, 958, 1222, 1471, 1561, 1612
RseI	CAYNNNNRTG	1 cut(s)	1520
SalI	GTCGAC	1 cut(s)	1488
SapI	GCTCTTC	2 cut(s)	911, 1493
SaqAI	TTAA	7 cut(s)	341, 470, 531, 678, 837, 1074, 1737
SatI	GCNGC	7 cut(s)	663, 755, 763, 1172, 1210, 1368, 1444
Sau96I	GGNCC	3 cut(s)	856, 907, 1016
SchI	GAGTC	4 cut(s)	256, 309, 1758, 1760
ScrFI	CCNGG	9 cut(s)	12, 59, 686, 707, 911, 1265, 1364, 1414, 1451
SduI	GDGCHC	4 cut(s)	57, 219, 1158, 1425
SfaNI	GCATC	2 cut(s)	1669, 1674
SfcI	CTRYAG	5 cut(s)	406, 1092, 1249, 1330, 1699
SinI	GGWCC	1 cut(s)	907
SmiMI	CAYNNNNRTG	1 cut(s)	1520
SmlI	CTYRAG	2 cut(s)	615, 836
SmoI	CTYRAG	2 cut(s)	615, 836
Sse9I	AATT	6 cut(s)	198, 444, 679, 1071, 1204, 1255
SspMI	CTAG	4 cut(s)	627, 1056, 1484, 1662
StyD4I	CCNGG	9 cut(s)	10, 57, 684, 705, 909, 1263, 1362, 1412, 1449
StyI	CCWWGG	5 cut(s)	66, 642, 1116, 1384, 1483
TaaI	ACNGT	2 cut(s)	853, 1782
TaiI	ACGT	4 cut(s)	849, 945, 1242, 1598
TaqI	TCGA	1 cut(s)	1489
TasI	AATT	6 cut(s)	198, 444, 679, 1071, 1204, 1255
TatI	WGTACW	4 cut(s)	876, 957, 1470, 1611
TauI	GCSGC	4 cut(s)	665, 1212, 1370, 1446
TfiI	GAWTC	2 cut(s)	307, 1546
Tru1I	TTAA	7 cut(s)	341, 470, 531, 678, 837, 1074, 1737
Tru9I	TTAA	7 cut(s)	341, 470, 531, 678, 837, 1074, 1737
TscAI	CASTG	4 cut(s)	664, 799, 1084, 1285
TseFI	GTSAC	5 cut(s)	847, 970, 1375, 1408, 1527
TseI	GCWGC	3 cut(s)	754, 762, 1171
Tsp45I	GTSAC	5 cut(s)	847, 970, 1375, 1408, 1527
TspDTI	ATGAA	4 cut(s)	159, 608, 612, 1285
TspGWI	ACGGA	2 cut(s)	165, 1586
TspRI	CASTG	4 cut(s)	664, 799, 1084, 1285
Tth111I	GACNNNGTC	1 cut(s)	1310
Van91I	CCANNNNNTGG	1 cut(s)	1165
Vha464I	CTTAAG	1 cut(s)	836
VpaK11BI	GGWCC	1 cut(s)	907
XapI	RAATTY	1 cut(s)	444
XceI	RCATGY	1 cut(s)	1642
XmaJI	CCTAGG	1 cut(s)	1483
XmiI	GTMKAC	1 cut(s)	1489
XmnI	GAANNNNTTC	2 cut(s)	1259, 1502
XspI	CTAG	4 cut(s)	627, 1056, 1484, 1662
ZraI	GACGTC	1 cut(s)	1596

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.