RchiOBHm_Chr3g0456331

Acts in the modification of cell walls via demethylesterification of cell wall pectin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000809
Physical Location & Seq
Reverse (-)
5891950 .. 5893827
1878 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42317

Sequence Viewer

Length: 1803 bp
ATGGCGTACGAGGAATCAAGTAGCTCAAAAAAAAGGTTTGCCATTATTGGCATCTCTTCGACACTATTAATAGCACTAGTGGTGGCCGCGACTGTTAGTGTTGGCCTAAAAACCACTACCCATGACAACGGCAGCAGGACTTCTGTCAAGTCACATGAGATATCTGCCTCCATGAAAGCCATAATTTCCATTTGCCAGCCCACCAACTACAAACAAGAATGTGTGAATAGCCTCCTATCCGCATCTGGAAACACCACCGACCCAAAAAAGCTCATTCAGGTTGGATTCCAAGTTGCAATGAAACAAATCTCCGTGGCCGCGAAGAACTCCACTCTCTTGCAACAGCTTGAGAAGGACCCTAGAGCTACCAAGGCCCTTGACACATGCAAAGAACTCTTGGGATTGGCTGTTACCGAGCTCAAGCAAGCTTTCGACAGATTGGGGGGTGACTTTGACATCACTGAAATTGATACAATGATCATGGATTTGAAAGTCTGGATAAGCGCTACTGTCACATACCAAGAGACATGCTTGGACGCATTTGAGAATAGTACTAGTGATGCCGGTGAGAAGATGAAGATGATATTGAAGAGCTCCATGCATTTGAGCAGCAACTCCCTTGCCATGGTCTCCACAACCTCTTCATTCCTCAGTGGCTTAGACCACAAAGGCCTAAGCCGCCAGCTCCTTGCGGATGATGGCCTCCCTGTTCTTGGTCACGAGGATTTTGAACTTCCTGATGATTTTAATAATCTTAATGATCTTAATAATACATGGTATTGGCCTGGATTCAGGAAACTCCTTACTGCTGCTAGTAGAGCTGATGTATACAAAGTTAAAAAGGCCGCGAACATTATAGTGGCCAAGGATGGAAGTGGAAAGTACAAGACCATCAATGAGGCCCTACATCATGTACCTAAGCATAGCAACATGACCTTTGTCATCTACATCAAGGAAGGTGTCTACAATGAGCTTGTCCACATTACCAAGGCCATGACCAATCTGATGCTGATCGGAGACGGTGCTAATAAGACCAGAATCACCGGAAACTTGAACTTCGTAGACGGCACCCCTACCTACAAAACCGCTACAGTTGGTATGTTTGTCTCGGTCCATGGAGCTCACTTCATGGCTAAGGATATTGGGTTTGAGAACTCAGCCGGTCCAGACAAACATCAAGCTGTGGCAATTAGGGTGAGTGCCGATGAGGCAATCTTCTATAGGTGTGCATTTGATGGCTACCAAGACACACTTTACACACATGTCCAACGCCAATTCTACCGTGACTGCACAATCTCCGGTACCATTGACTTTGTTTTCGGTGATGCTGCCGCCATTTTCCAAAACTGCACTTTTGTGGTGCGCAAGCCAATGCCGAACCAGTCTTGCATTGTCACAGCTCAAGGCCGGAAAGAGAGGCGCCAGCCAACTGGAATCATTATTATAAACAGCAGATTTACCTCTGATGCCGCATACTACCCTCTGAGGCATGAAAACAAGGCATATCTTGGCCGTCCATGGAAGGAGTACTCGAGGACCATCATCATGGAGTCATACATTGATGACTTCATTCGGCCACAAGGTTGGTTACCCTGGGAGGCCGATTTCGGGCTCAAGACATGCTTCTATGCTGAGTACAACAATCGTGGCCCTGGTGCTAGCAAGGCGAAGCGTGTGACATGGCACTGCATCAAGAACATTACGCCGCAGCATGCTTTGGATTTCACACCCGGCAGGTTCTTTAAGGGTGATCGATGGGTTAGGCTATCTGGAGTGCCTTATTTTCCTGGCCTCACTGCTTGA

Protein Analysis

600

Amino Acids

66.73

Weight (kDa)

8.76

Isoelectric Point (pI)

29.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 60 - 209 6.5e-29 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 284 - 584 3.6e-137 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1445
Acc16I TGCGCA 1 cut(s) 1364
Acc36I ACCTGC 1 cut(s) 1725
Acc65I GGTACC 1 cut(s) 1301
AccB1I GGYRCC 3 cut(s) 1067, 1301, 1419
AccI GTMKAC 3 cut(s) 828, 963, 1062
AccII CGCG 3 cut(s) 89, 320, 848
AcoI YGGCCR 5 cut(s) 84, 315, 861, 1510, 1574
AcyI GRCGYC 1 cut(s) 1420
AfaI GTAC 7 cut(s) 8, 553, 884, 915, 1303, 1529, 1637
AfeI AGCGCT 1 cut(s) 505
AfiI CCNNNNNNNGG 3 cut(s) 625, 713, 1608
AflIII ACRYGT 1 cut(s) 1261
AgsI TTSAA 4 cut(s) 490, 589, 731, 1054
AhlI ACTAGT 2 cut(s) 76, 554
AjnI CCWGG 4 cut(s) 784, 1592, 1651, 1786
AleI CACNNNNGTG 1 cut(s) 1355
Alw21I GWGCWC 3 cut(s) 420, 596, 1123
Alw26I GTCTC 4 cut(s) 518, 634, 1011, 1111
Ama87I CYCGRG 1 cut(s) 1531
Aor51HI AGCGCT 1 cut(s) 505
ApeKI GCWGC 5 cut(s) 132, 609, 809, 1328, 1708
ArsI GACNNNNNNTTYG 2 cut(s) 1301, 1333
AseI ATTAAT 1 cut(s) 68
Asp718I GGTACC 1 cut(s) 1301
AspLEI GCGC 3 cut(s) 506, 1365, 1422
AspS9I GGNCC 7 cut(s) 355, 373, 901, 1111, 1163, 1536, 1649
AsuC2I CCSGG 1 cut(s) 1731
AsuHPI GGTGA 6 cut(s) 458, 578, 1033, 1207, 1334, 1760
AsuNHI GCTAGC 1 cut(s) 1658
AvaI CYCGRG 1 cut(s) 1531
AvaII GGWCC 4 cut(s) 355, 1111, 1163, 1536
BalI TGGCCA 1 cut(s) 863
BanI GGYRCC 3 cut(s) 1067, 1301, 1419
BanII GRGCYC 4 cut(s) 420, 596, 1123, 1614
BauI CACGAG 1 cut(s) 719
Bbv12I GWGCWC 3 cut(s) 420, 596, 1123
BbvI GCAGC 5 cut(s) 144, 621, 796, 1315, 1720
BccI CCATC 6 cut(s) 692, 863, 899, 1229, 1547, 1749
BceAI ACGGC 3 cut(s) 145, 1081, 1497
BciT130I CCWGG 4 cut(s) 786, 1594, 1653, 1788
BclI TGATCA 1 cut(s) 477
BcnI CCSGG 1 cut(s) 1731
BcoDI GTCTC 4 cut(s) 518, 634, 1011, 1111
BcuI ACTAGT 2 cut(s) 76, 554
BfaI CTAG 5 cut(s) 77, 360, 555, 813, 1659
BfmI CTRYAG 2 cut(s) 1089, 1219
BfoI RGCGCY 2 cut(s) 507, 1423
BfuAI ACCTGC 1 cut(s) 1725
BglI GCCNNNNNGGC 1 cut(s) 1208
BmcAI AGTACT 2 cut(s) 553, 1529
Bme1390I CCNGG 5 cut(s) 786, 1594, 1653, 1731, 1788
Bme18I GGWCC 4 cut(s) 355, 1111, 1163, 1536
BmeT110I CYCGRG 1 cut(s) 1531
BmgT120I GGNCC 7 cut(s) 355, 373, 901, 1111, 1163, 1536, 1649
BmiI GGNNCC 4 cut(s) 357, 1069, 1303, 1421
BmrFI CCNGG 5 cut(s) 786, 1594, 1653, 1731, 1788
BmsI GCATC 7 cut(s) 60, 251, 550, 996, 1315, 1456, 1698
BmtI GCTAGC 1 cut(s) 1662
BoxI GACNNNNGTC 2 cut(s) 143, 938
BpmI CTGGAG 1 cut(s) 1791
Bpu10I CCTNAGC 3 cut(s) 674, 918, 1134
BpuEI CTTGAG 4 cut(s) 368, 404, 1386, 1598
BpuMI CCSGG 1 cut(s) 1731
Bsa29I ATCGAT 1 cut(s) 1753
BsaBI GATNNNNATC 1 cut(s) 1010
BsaHI GRCGYC 1 cut(s) 1420
BsaI GGTCTC 1 cut(s) 634
BsaWI WCCGGW 2 cut(s) 1043, 1298
Bsc4I CCNNNNNNNGG 3 cut(s) 625, 713, 1608
Bse118I RCCGGY 2 cut(s) 563, 1160
Bse1I ACTGG 2 cut(s) 1381, 1435
Bse3DI GCAATG 1 cut(s) 303
Bse8I GATNNNNATC 1 cut(s) 1010
BseBI CCWGG 4 cut(s) 786, 1594, 1653, 1788
BseCI ATCGAT 1 cut(s) 1753
BseGI GGATG 2 cut(s) 700, 874
BseJI GATNNNNATC 1 cut(s) 1010
BseLI CCNNNNNNNGG 3 cut(s) 625, 713, 1608
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 4 cut(s) 664, 1170, 1475, 1623
BseNI ACTGG 2 cut(s) 1381, 1435
BseXI GCAGC 5 cut(s) 144, 621, 796, 1315, 1720
BsgI GTGCAG 2 cut(s) 1273, 1333
Bsh1236I CGCG 3 cut(s) 89, 320, 848
BshNI GGYRCC 3 cut(s) 1067, 1301, 1419
BshVI ATCGAT 1 cut(s) 1753
BsiHKAI GWGCWC 3 cut(s) 420, 596, 1123
BsiHKCI CYCGRG 1 cut(s) 1531
BsiSI CCGG 6 cut(s) 564, 1044, 1161, 1299, 1408, 1731
BsiWI CGTACG 1 cut(s) 6
BslI CCNNNNNNNGG 3 cut(s) 625, 713, 1608
BsmAI GTCTC 4 cut(s) 518, 634, 1011, 1111
BsmBI CGTCTC 1 cut(s) 1011
Bso31I GGTCTC 1 cut(s) 634
BsoBI CYCGRG 1 cut(s) 1531
Bsp1286I GDGCHC 4 cut(s) 420, 596, 1123, 1614
Bsp143I GATC 4 cut(s) 477, 760, 1011, 1750
Bsp19I CCATGG 3 cut(s) 624, 1114, 1517
BspCNI CTCAG 4 cut(s) 663, 1169, 1476, 1624
BspDI ATCGAT 1 cut(s) 1753
BspFNI CGCG 3 cut(s) 89, 320, 848
BspLI GGNNCC 4 cut(s) 357, 1069, 1303, 1421
BspMI ACCTGC 1 cut(s) 1725
BspOI GCTAGC 1 cut(s) 1662
BspQI GCTCTTC 1 cut(s) 584
BspT107I GGYRCC 3 cut(s) 1067, 1301, 1419
BspTNI GGTCTC 1 cut(s) 634
BsrDI GCAATG 1 cut(s) 303
BsrFI RCCGGY 2 cut(s) 563, 1160
BsrI ACTGG 2 cut(s) 1381, 1435
BssAI RCCGGY 2 cut(s) 563, 1160
BssMI GATC 4 cut(s) 477, 760, 1011, 1750
BssNAI GTATAC 1 cut(s) 829
BssNI GRCGYC 1 cut(s) 1420
BssSI CACGAG 1 cut(s) 719
BssT1I CCWWGG 6 cut(s) 369, 624, 864, 987, 1114, 1517
Bst1107I GTATAC 1 cut(s) 829
Bst2BI CACGAG 1 cut(s) 719
Bst2UI CCWGG 4 cut(s) 786, 1594, 1653, 1788
Bst4CI ACNGT 5 cut(s) 94, 511, 1022, 1093, 1283
Bst6I CTCTTC 3 cut(s) 61, 584, 646
BstACI GRCGYC 1 cut(s) 1420
BstC8I GCNNGC 7 cut(s) 197, 426, 683, 1367, 1424, 1660, 1713
BstDEI CTNAG 8 cut(s) 650, 658, 674, 918, 1134, 1156, 1484, 1632
BstDSI CCRYGG 4 cut(s) 312, 624, 1114, 1517
BstEII GGTNACC 1 cut(s) 1587
BstF5I GGATG 2 cut(s) 700, 874
BstFNI CGCG 3 cut(s) 89, 320, 848
BstH2I RGCGCY 2 cut(s) 507, 1423
BstHHI GCGC 3 cut(s) 506, 1365, 1422
BstKTI GATC 4 cut(s) 480, 763, 1014, 1753
BstMAI GTCTC 4 cut(s) 518, 634, 1011, 1111
BstMBI GATC 4 cut(s) 477, 760, 1011, 1750
BstMWI GCNNNNNNNGC 5 cut(s) 371, 678, 818, 1208, 1664
BstNI CCWGG 4 cut(s) 786, 1594, 1653, 1788
BstNSI RCATGY 5 cut(s) 387, 531, 1265, 1623, 1715
BstPAI GACNNNNGTC 2 cut(s) 143, 938
BstPI GGTNACC 1 cut(s) 1587
BstSCI CCNGG 5 cut(s) 784, 1592, 1651, 1729, 1786
BstSFI CTRYAG 2 cut(s) 1089, 1219
BstUI CGCG 3 cut(s) 89, 320, 848
BstV1I GCAGC 5 cut(s) 144, 621, 796, 1315, 1720
BstXI CCANNNNNNTGG 3 cut(s) 1430, 1546, 1584
BstZ17I GTATAC 1 cut(s) 829
Bsu15I ATCGAT 1 cut(s) 1753
BsuTUI ATCGAT 1 cut(s) 1753
BtgI CCRYGG 4 cut(s) 312, 624, 1114, 1517
BtsCI GGATG 2 cut(s) 700, 874
BtsI GCAGTG 2 cut(s) 1684, 1794
BtsIMutI CAGTG 4 cut(s) 459, 658, 1684, 1794
BveI ACCTGC 1 cut(s) 1725
Cac8I GCNNGC 7 cut(s) 197, 426, 683, 1367, 1424, 1660, 1713
CfoI GCGC 3 cut(s) 506, 1365, 1422
Cfr10I RCCGGY 2 cut(s) 563, 1160
Cfr13I GGNCC 7 cut(s) 355, 373, 901, 1111, 1163, 1536, 1649
ClaI ATCGAT 1 cut(s) 1753
CseI GACGC 1 cut(s) 545
Csp6I GTAC 7 cut(s) 7, 552, 883, 914, 1302, 1528, 1636
CspCI CAANNNNNGTGG 2 cut(s) 1627, 1662
CviQI GTAC 7 cut(s) 7, 552, 883, 914, 1302, 1528, 1636
DdeI CTNAG 8 cut(s) 650, 658, 674, 918, 1134, 1156, 1484, 1632
DinI GGCGCC 1 cut(s) 1421
DpnI GATC 4 cut(s) 479, 762, 1013, 1752
DpnII GATC 4 cut(s) 477, 760, 1011, 1750
EaeI YGGCCR 5 cut(s) 84, 315, 861, 1510, 1574
Eam1104I CTCTTC 3 cut(s) 61, 584, 646
EarI CTCTTC 3 cut(s) 61, 584, 646
Ecl136II GAGCTC 3 cut(s) 418, 594, 1121
Eco130I CCWWGG 6 cut(s) 369, 624, 864, 987, 1114, 1517
Eco147I AGGCCT 1 cut(s) 672
Eco24I GRGCYC 4 cut(s) 420, 596, 1123, 1614
Eco31I GGTCTC 1 cut(s) 634
Eco32I GATATC 1 cut(s) 162
Eco47I GGWCC 4 cut(s) 355, 1111, 1163, 1536
Eco47III AGCGCT 1 cut(s) 505
Eco53kI GAGCTC 3 cut(s) 418, 594, 1121
Eco88I CYCGRG 1 cut(s) 1531
Eco91I GGTNACC 1 cut(s) 1587
EcoICRI GAGCTC 3 cut(s) 418, 594, 1121
EcoO109I RGGNCCY 3 cut(s) 355, 373, 901
EcoO65I GGTNACC 1 cut(s) 1587
EcoRII CCWGG 4 cut(s) 784, 1592, 1651, 1786
EcoRV GATATC 1 cut(s) 162
EcoT14I CCWWGG 6 cut(s) 369, 624, 864, 987, 1114, 1517
EcoT22I ATGCAT 1 cut(s) 603
EcoT38I GRGCYC 4 cut(s) 420, 596, 1123, 1614
EgeI GGCGCC 1 cut(s) 1421
EheI GGCGCC 1 cut(s) 1421
ErhI CCWWGG 6 cut(s) 369, 624, 864, 987, 1114, 1517
Esp3I CGTCTC 1 cut(s) 1011
FalI AAGNNNNNCTT 4 cut(s) 1236, 1268, 1607, 1639
FbaI TGATCA 1 cut(s) 477
FblI GTMKAC 3 cut(s) 828, 963, 1062
FokI GGATG 2 cut(s) 707, 881
FriOI GRGCYC 4 cut(s) 420, 596, 1123, 1614
FspBI CTAG 5 cut(s) 77, 360, 555, 813, 1659
FspI TGCGCA 1 cut(s) 1364
GlaI GCGC 3 cut(s) 505, 1364, 1421
GsuI CTGGAG 1 cut(s) 1791
HaeII RGCGCY 2 cut(s) 507, 1423
HapII CCGG 6 cut(s) 564, 1044, 1161, 1299, 1408, 1731
HgaI GACGC 1 cut(s) 545
HhaI GCGC 3 cut(s) 506, 1365, 1422
Hin1I GRCGYC 1 cut(s) 1420
Hin6I GCGC 3 cut(s) 504, 1363, 1420
HinP1I GCGC 3 cut(s) 504, 1363, 1420
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 6 cut(s) 14, 285, 789, 1038, 1434, 1550
HpaII CCGG 6 cut(s) 564, 1044, 1161, 1299, 1408, 1731
HphI GGTGA 6 cut(s) 458, 578, 1033, 1207, 1334, 1760
Hpy166II GTNNAC 4 cut(s) 829, 964, 979, 1063
Hpy188I TCNGA 4 cut(s) 1005, 1016, 1465, 1485
Hpy188III TCNNGA 9 cut(s) 246, 496, 719, 737, 793, 1166, 1615, 1693, 1770
Hpy8I GTNNAC 4 cut(s) 829, 964, 979, 1063
HpyAV CCTTC 3 cut(s) 346, 950, 1516
HpyCH4III ACNGT 5 cut(s) 94, 511, 1022, 1093, 1283
HpyCH4V TGCA 9 cut(s) 296, 340, 387, 601, 1229, 1290, 1350, 1389, 1689
HpyF10VI GCNNNNNNNGC 5 cut(s) 371, 678, 818, 1208, 1664
HpyF3I CTNAG 8 cut(s) 650, 658, 674, 918, 1134, 1156, 1484, 1632
Hsp92I GRCGYC 1 cut(s) 1420
HspAI GCGC 3 cut(s) 504, 1363, 1420
KasI GGCGCC 1 cut(s) 1419
KpnI GGTACC 1 cut(s) 1305
Ksp22I TGATCA 1 cut(s) 477
Kzo9I GATC 4 cut(s) 477, 760, 1011, 1750
LguI GCTCTTC 1 cut(s) 584
LmnI GCTCC 3 cut(s) 599, 690, 1118
Lsp1109I GCAGC 5 cut(s) 144, 621, 796, 1315, 1720
LweI GCATC 7 cut(s) 60, 251, 550, 996, 1315, 1456, 1698
MaeI CTAG 5 cut(s) 77, 360, 555, 813, 1659
MaeIII GTNAC 9 cut(s) 150, 409, 446, 511, 716, 1283, 1393, 1587, 1675
MalI GATC 4 cut(s) 479, 762, 1013, 1752
MboI GATC 4 cut(s) 477, 760, 1011, 1750
MboII GAAGA 7 cut(s) 48, 334, 583, 589, 601, 633, 1207
MhlI GDGCHC 4 cut(s) 420, 596, 1123, 1614
MlsI TGGCCA 1 cut(s) 863
MluCI AATT 4 cut(s) 183, 465, 1188, 1274
MluNI TGGCCA 1 cut(s) 863
Mly113I GGCGCC 1 cut(s) 1420
MlyI GAGTC 1 cut(s) 1559
MmeI TCCRAC 2 cut(s) 262, 1291
Mox20I TGGCCA 1 cut(s) 863
Mph1103I ATGCAT 1 cut(s) 603
MscI TGGCCA 1 cut(s) 863
MseI TTAA 6 cut(s) 68, 747, 756, 765, 837, 1743
MslI CAYNNNNRTG 3 cut(s) 857, 1355, 1544
Msp20I TGGCCA 1 cut(s) 863
MspI CCGG 6 cut(s) 564, 1044, 1161, 1299, 1408, 1731
MspR9I CCNGG 5 cut(s) 786, 1594, 1653, 1731, 1788
MvaI CCWGG 4 cut(s) 786, 1594, 1653, 1788
MvnI CGCG 3 cut(s) 89, 320, 848
MwoI GCNNNNNNNGC 5 cut(s) 371, 678, 818, 1208, 1664
NarI GGCGCC 1 cut(s) 1420
NciI CCSGG 1 cut(s) 1731
NcoI CCATGG 3 cut(s) 624, 1114, 1517
NdeII GATC 4 cut(s) 477, 760, 1011, 1750
NheI GCTAGC 1 cut(s) 1658
NlaIV GGNNCC 4 cut(s) 357, 1069, 1303, 1421
NmuCI GTSAC 7 cut(s) 150, 446, 511, 716, 1283, 1393, 1675
NsbI TGCGCA 1 cut(s) 1364
NsiI ATGCAT 1 cut(s) 603
NspI RCATGY 5 cut(s) 387, 531, 1265, 1623, 1715
OliI CACNNNNGTG 1 cut(s) 1355
PaeI GCATGC 1 cut(s) 1715
PaeR7I CTCGAG 1 cut(s) 1531
PasI CCCWGGG 1 cut(s) 1593
PceI AGGCCT 1 cut(s) 672
PciI ACATGT 1 cut(s) 1261
PciSI GCTCTTC 1 cut(s) 584
PfeI GAWTC 5 cut(s) 14, 285, 789, 1038, 1434
Pfl23II CGTACG 1 cut(s) 6
PleI GAGTC 1 cut(s) 1558
PluTI GGCGCC 1 cut(s) 1423
PpsI GAGTC 1 cut(s) 1558
PpuMI RGGWCCY 1 cut(s) 355
PscI ACATGT 1 cut(s) 1261
PshAI GACNNNNGTC 2 cut(s) 143, 938
PshBI ATTAAT 1 cut(s) 68
PsiI TTATAA 1 cut(s) 1445
Psp124BI GAGCTC 3 cut(s) 420, 596, 1123
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 4 cut(s) 784, 1592, 1651, 1786
PspEI GGTNACC 1 cut(s) 1587
PspGI CCWGG 4 cut(s) 784, 1592, 1651, 1786
PspLI CGTACG 1 cut(s) 6
PspN4I GGNNCC 4 cut(s) 357, 1069, 1303, 1421
PspPI GGNCC 7 cut(s) 355, 373, 901, 1111, 1163, 1536, 1649
PspPPI RGGWCCY 1 cut(s) 355
PspXI VCTCGAGB 1 cut(s) 1531
RsaI GTAC 7 cut(s) 8, 553, 884, 915, 1303, 1529, 1637
RsaNI GTAC 7 cut(s) 7, 552, 883, 914, 1302, 1528, 1636
RseI CAYNNNNRTG 3 cut(s) 857, 1355, 1544
SacI GAGCTC 3 cut(s) 420, 596, 1123
SapI GCTCTTC 1 cut(s) 584
SaqAI TTAA 6 cut(s) 68, 747, 756, 765, 837, 1743
Sau3AI GATC 4 cut(s) 477, 760, 1011, 1750
Sau96I GGNCC 7 cut(s) 355, 373, 901, 1111, 1163, 1536, 1649
ScaI AGTACT 2 cut(s) 553, 1529
SchI GAGTC 1 cut(s) 1559
ScrFI CCNGG 5 cut(s) 786, 1594, 1653, 1731, 1788
SduI GDGCHC 4 cut(s) 420, 596, 1123, 1614
SfaNI GCATC 7 cut(s) 60, 251, 550, 996, 1315, 1456, 1698
SfcI CTRYAG 2 cut(s) 1089, 1219
SfoI GGCGCC 1 cut(s) 1421
Sfr274I CTCGAG 1 cut(s) 1531
SinI GGWCC 4 cut(s) 355, 1111, 1163, 1536
SlaI CTCGAG 1 cut(s) 1531
SmiMI CAYNNNNRTG 3 cut(s) 857, 1355, 1544
SmlI CTYRAG 5 cut(s) 347, 419, 1401, 1531, 1613
SmoI CTYRAG 5 cut(s) 347, 419, 1401, 1531, 1613
SpeI ACTAGT 2 cut(s) 76, 554
SphI GCATGC 1 cut(s) 1715
Sse9I AATT 4 cut(s) 183, 465, 1188, 1274
SseBI AGGCCT 1 cut(s) 672
SspDI GGCGCC 1 cut(s) 1419
SspMI CTAG 5 cut(s) 77, 360, 555, 813, 1659
SstI GAGCTC 3 cut(s) 420, 596, 1123
StuI AGGCCT 1 cut(s) 672
StyD4I CCNGG 5 cut(s) 784, 1592, 1651, 1729, 1786
StyI CCWWGG 6 cut(s) 369, 624, 864, 987, 1114, 1517
TaaI ACNGT 5 cut(s) 94, 511, 1022, 1093, 1283
TaqI TCGA 4 cut(s) 59, 432, 1532, 1753
TaqII GACCGA 1 cut(s) 1099
TasI AATT 4 cut(s) 183, 465, 1188, 1274
TatI WGTACW 4 cut(s) 551, 882, 1527, 1635
TauI GCSGC 7 cut(s) 89, 320, 681, 848, 1334, 1472, 1708
TfiI GAWTC 5 cut(s) 14, 285, 789, 1038, 1434
Tru1I TTAA 6 cut(s) 68, 747, 756, 765, 837, 1743
Tru9I TTAA 6 cut(s) 68, 747, 756, 765, 837, 1743
TscAI CASTG 4 cut(s) 466, 658, 1691, 1801
TseFI GTSAC 7 cut(s) 150, 446, 511, 716, 1283, 1393, 1675
TseI GCWGC 5 cut(s) 132, 609, 809, 1328, 1708
Tsp45I GTSAC 7 cut(s) 150, 446, 511, 716, 1283, 1393, 1675
TspDTI ATGAA 7 cut(s) 188, 314, 590, 633, 1117, 1506, 1558
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 4 cut(s) 466, 658, 1691, 1801
VpaK11BI GGWCC 4 cut(s) 355, 1111, 1163, 1536
VspI ATTAAT 1 cut(s) 68
XceI RCATGY 5 cut(s) 387, 531, 1265, 1623, 1715
XhoI CTCGAG 1 cut(s) 1531
XmiI GTMKAC 3 cut(s) 828, 963, 1062
XspI CTAG 5 cut(s) 77, 360, 555, 813, 1659
ZrmI AGTACT 2 cut(s) 553, 1529
Zsp2I ATGCAT 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.