Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Forward (+)

13577393 .. 13579788

Gene Structure 2396 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1164300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1722 bp

ATGGGCGGAGCAGACGGATCTAACAAAAAAAAAGTCGCCATCATTGGCGCCTCCGCACTGATTCTGGTGGCAATGGTAGTTGCTGTAACTGTTGGGGTCACAGTGTCACGCCGCGACAGAGGGTCGTCTGACGGCCACACCTCCACGTCCACAAAGGCGGTCCAGGCCATTTGCCAGCCCACAGACTACAAAAAGACCTGCGAGGAAAGCCTTGCAGGCGCGGCCGGCAACGTCACCGATCCCAAAGAGCTTATCAAAGTAGGGTTCCAAGTCGCCATGGACAAGCTCCGCGGTGTCATCGAGAAGTCCGTCACGTTGAAGGAGCTGGCCAAGGACCCGAGCACAGGCGACGCACTCCAGAACTGCAAGGAGCTCCTGGAGTACGCCATCGATGATTTGAGTGACTCGTCCGAAAGGCTCGGGGCCTTTGACATGAGCAAGCTCGATCTTTACGTGGAGGATCTCAAGGTGTGGCTCAGCGCCGCCATGACATACGAGCAGACTTGCTTAGATGGGTTTGAGAACACCACCGGTGACGCCGGTGAGAAGATGAGACAGTTCTTGAAGACATCGCAAGAGCTCACCAATAACGCCCTTGCCATGGCCGATGGAGTTTCCACGATGTTCGGGGCTCTTAATGTTAAATCCGGCCGCAGACTTCTTGAGGCTGCAGCTGGAACTGCTGTGAAGAAGTTCCAAAAGGCTAAGGTCATTCCCGCTTGGATTGATAGCCGAAGGCTTGAATTGGCTACCGCCACTCCTCAGACGCTGAAACCCGATGTGGTGGTGGCCAAGAACGGGGGTGGTAAGTACAAAACTGTCAACGAAGCCTTGAAGGATATCCCAAAGAACAACGCAGTGAAGGTCTTTGTGATTTATGTCAAGGAGGGAGTGTATGAGGAGCAGGTCATTTTTGATAAGCACATGACCAATGTCATGCTTATCGGAGATGGCCCCACTAAGACCGTGATCACCGGTAGAAAGAACTTTGCAGAGGGTACCATGACCATGCAGACTGCCACAGTTGGTGTTGTGGGAGACTACTTCATTGCCAAGGACATTGGATTTGAGAACACAGCCGGAGCCATTGGACACCAAGCCGTGGCTCTACGTGTCCAGTCAGACTTGTCCATCTTCTACAACTGCAAGATGGACGGGTACCAAGACACCCTGTACACCCAAACCTACAGGCAATTCTACCGCGACTGCACCATTAGTGGAACCATCGACTTCATCTTTGGTGATGCTGCCGCCGTGTTCCAAAACTGCAAGATGATCGTCAGGAAGCCACTCGAGAACCAGGCCTGCATGGTGACTGCCCACGGGAGACTCGACAGGCGTTCTCCCTCGGGTATCACCATCCAAAACTGCACCATCTCTGGTGAACTAGGCTACGAGAAAGACCTCAATAAGGCATACTTGGGCAGACCATGGAAGAAGTACGCAAGGACCATCGTCATACAGTCCCAGATCGATGACGTGATTGCTCCAGAAGGGTGGATGGATTGGATTGGATCAAACAATCACCAATCTTGCTGGTTCGGTGAGTTTGGCAATAGAGGACTCGGTGCCGACCAGACTAGAAGGGCGACATGGCGTGGAATCAAGAAGCTTAACGCTCAGCACGCCGCGGACTTCACCGCAGGGAGGTTCGTGTTCGGTGATAGATGGATTCAGCCTAGCGGTGTGCCCTACGTCGCTGGCATGATGCCCGGAGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

574

Amino Acids

62.53

Weight (kDa)

8.48

Isoelectric Point (pI)

29.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	53 - 201	1.8e-33	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	260 - 554	3.4e-125	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	206, 895
Acc65I	GGTACC	2 cut(s)	998, 1158
AccB1I	GGYRCC	4 cut(s)	47, 998, 1158, 1568
AccB7I	CCANNNNNTGG	1 cut(s)	1102
AccII	CGCG	5 cut(s)	114, 221, 291, 1203, 1631
AclWI	GGATC	4 cut(s)	25, 233, 468, 1522
AcoI	YGGCCR	6 cut(s)	133, 222, 327, 603, 649, 789
AcyI	GRCGYC	2 cut(s)	48, 537
AfaI	GTAC	6 cut(s)	383, 812, 1000, 1160, 1175, 1442
AfiI	CCNNNNNNNGG	6 cut(s)	344, 601, 798, 1102, 1411, 1647
AflIII	ACRYGT	1 cut(s)	1111
AgeI	ACCGGT	2 cut(s)	530, 974
AgsI	TTSAA	4 cut(s)	319, 565, 743, 835
AhdI	GACNNNNNGTC	1 cut(s)	121
AjiI	CACGTC	2 cut(s)	147, 1480
AjnI	CCWGG	3 cut(s)	162, 375, 1299
AluBI	AGCT	8 cut(s)	250, 286, 325, 373, 442, 580, 674, 1612
AluI	AGCT	8 cut(s)	250, 286, 325, 373, 442, 580, 674, 1612
Alw21I	GWGCWC	3 cut(s)	344, 375, 582
Alw26I	GTCTC	3 cut(s)	547, 1032, 1321
AlwI	GGATC	4 cut(s)	25, 233, 468, 1522
AlwNI	CAGNNNCTG	1 cut(s)	769
Ama87I	CYCGRG	4 cut(s)	337, 419, 1292, 1348
ApeKI	GCWGC	3 cut(s)	668, 671, 1247
ArsI	GACNNNNNNTTYG	6 cut(s)	18, 50, 1049, 1081, 1220, 1252
AsiGI	ACCGGT	2 cut(s)	530, 974
Asp700I	GAANNNNTTC	1 cut(s)	692
Asp718I	GGTACC	2 cut(s)	998, 1158
AspLEI	GCGC	3 cut(s)	50, 221, 482
AspS9I	GGNCC	5 cut(s)	160, 334, 423, 953, 1449
AsuC2I	CCSGG	1 cut(s)	1713
AvaI	CYCGRG	4 cut(s)	337, 419, 1292, 1348
AvaII	GGWCC	3 cut(s)	160, 334, 1449
BaeGI	GKGCMC	1 cut(s)	1692
BalI	TGGCCA	2 cut(s)	329, 791
BanI	GGYRCC	4 cut(s)	47, 998, 1158, 1568
BanII	GRGCYC	3 cut(s)	375, 582, 634
BbsI	GAAGAC	1 cut(s)	572
Bbv12I	GWGCWC	3 cut(s)	344, 375, 582
BbvI	GCAGC	3 cut(s)	655, 683, 1234
BceAI	ACGGC	3 cut(s)	148, 1085, 1238
BcgI	CGANNNNNNTGC	2 cut(s)	1258, 1292
BciT130I	CCWGG	3 cut(s)	164, 377, 1301
BclI	TGATCA	1 cut(s)	969
BcnI	CCSGG	1 cut(s)	1713
BcoDI	GTCTC	3 cut(s)	547, 1032, 1321
BfaI	CTAG	3 cut(s)	1388, 1581, 1680
BfmI	CTRYAG	2 cut(s)	669, 1186
BfoI	RGCGCY	2 cut(s)	51, 483
BfuAI	ACCTGC	2 cut(s)	206, 895
BglI	GCCNNNNNGGC	1 cut(s)	216
BisI	GCNGC	9 cut(s)	112, 222, 483, 652, 669, 672, 1248, 1251, 1629
BlpI	GCTNAGC	2 cut(s)	476, 1620
BlsI	GCNGC	9 cut(s)	113, 223, 484, 653, 670, 673, 1249, 1252, 1630
Bme1390I	CCNGG	4 cut(s)	164, 377, 1301, 1713
Bme18I	GGWCC	3 cut(s)	160, 334, 1449
BmeRI	GACNNNNNGTC	1 cut(s)	121
BmeT110I	CYCGRG	4 cut(s)	337, 419, 1292, 1348
BmgBI	CACGTC	2 cut(s)	147, 1480
BmgT120I	GGNCC	5 cut(s)	160, 334, 423, 953, 1449
BmrFI	CCNGG	4 cut(s)	164, 377, 1301, 1713
BmsI	GCATC	2 cut(s)	1234, 1698
BoxI	GACNNNNGTC	2 cut(s)	932, 1454
BpiI	GAAGAC	1 cut(s)	572
BpmI	CTGGAG	3 cut(s)	341, 398, 1473
Bpu10I	CCTNAGC	1 cut(s)	705
Bpu1102I	GCTNAGC	2 cut(s)	476, 1620
BpuEI	CTTGAG	2 cut(s)	449, 683
BpuMI	CCSGG	1 cut(s)	1713
Bsa29I	ATCGAT	2 cut(s)	390, 1473
BsaAI	YACGTR	2 cut(s)	454, 1112
BsaHI	GRCGYC	2 cut(s)	48, 537
BsaWI	WCCGGW	2 cut(s)	530, 974
BsaXI	ACNNNNNCTCC	4 cut(s)	742, 772, 878, 908
Bsc4I	CCNNNNNNNGG	6 cut(s)	344, 601, 798, 1102, 1411, 1647
Bse118I	RCCGGY	4 cut(s)	224, 530, 539, 974
Bse1I	ACTGG	1 cut(s)	1117
Bse3DI	GCAATG	2 cut(s)	78, 1047
BseBI	CCWGG	3 cut(s)	164, 377, 1301
BseCI	ATCGAT	2 cut(s)	390, 1473
BseGI	GGATG	2 cut(s)	1359, 1506
BseLI	CCNNNNNNNGG	6 cut(s)	344, 601, 798, 1102, 1411, 1647
BseMI	GCAATG	2 cut(s)	78, 1047
BseMII	CTCAG	3 cut(s)	490, 776, 1634
BseNI	ACTGG	1 cut(s)	1117
BseRI	GAGGAG	2 cut(s)	750, 914
BseSI	GKGCMC	1 cut(s)	1692
BseX3I	CGGCCG	2 cut(s)	222, 649
BseXI	GCAGC	3 cut(s)	655, 683, 1234
BsgI	GTGCAG	2 cut(s)	1192, 1354
Bsh1236I	CGCG	5 cut(s)	114, 221, 291, 1203, 1631
Bsh1285I	CGRYCG	2 cut(s)	225, 652
BshNI	GGYRCC	4 cut(s)	47, 998, 1158, 1568
BshTI	ACCGGT	2 cut(s)	530, 974
BshVI	ATCGAT	2 cut(s)	390, 1473
BsiEI	CGRYCG	2 cut(s)	225, 652
BsiHKAI	GWGCWC	3 cut(s)	344, 375, 582
BsiHKCI	CYCGRG	4 cut(s)	337, 419, 1292, 1348
BsiSI	CCGG	7 cut(s)	225, 531, 540, 648, 975, 1080, 1713
BslFI	GGGAC	1 cut(s)	1450
BslI	CCNNNNNNNGG	6 cut(s)	344, 601, 798, 1102, 1411, 1647
BsmAI	GTCTC	3 cut(s)	547, 1032, 1321
BsmFI	GGGAC	1 cut(s)	1450
BsoBI	CYCGRG	4 cut(s)	337, 419, 1292, 1348
Bsp1286I	GDGCHC	5 cut(s)	344, 375, 582, 634, 1692
Bsp1407I	TGTACA	1 cut(s)	1173
Bsp143I	GATC	8 cut(s)	17, 238, 445, 460, 969, 1275, 1470, 1514
Bsp1720I	GCTNAGC	2 cut(s)	476, 1620
Bsp19I	CCATGG	3 cut(s)	276, 600, 1430
BspCNI	CTCAG	3 cut(s)	489, 775, 1633
BspDI	ATCGAT	2 cut(s)	390, 1473
BspFNI	CGCG	5 cut(s)	114, 221, 291, 1203, 1631
BspMAI	CTGCAG	1 cut(s)	673
BspMI	ACCTGC	2 cut(s)	206, 895
BspPI	GGATC	4 cut(s)	25, 233, 468, 1522
BspT107I	GGYRCC	4 cut(s)	47, 998, 1158, 1568
BsrDI	GCAATG	2 cut(s)	78, 1047
BsrFI	RCCGGY	4 cut(s)	224, 530, 539, 974
BsrGI	TGTACA	1 cut(s)	1173
BsrI	ACTGG	1 cut(s)	1117
BssAI	RCCGGY	4 cut(s)	224, 530, 539, 974
BssMI	GATC	8 cut(s)	17, 238, 445, 460, 969, 1275, 1470, 1514
BssNI	GRCGYC	2 cut(s)	48, 537
BssT1I	CCWWGG	5 cut(s)	276, 330, 600, 1053, 1430
Bst2UI	CCWGG	3 cut(s)	164, 377, 1301
Bst4CI	ACNGT	7 cut(s)	91, 103, 558, 820, 967, 1024, 1464
BstACI	GRCGYC	2 cut(s)	48, 537
BstAUI	TGTACA	1 cut(s)	1173
BstBAI	YACGTR	2 cut(s)	454, 1112
BstC8I	GCNNGC	8 cut(s)	176, 217, 226, 327, 440, 1306, 1626, 1702
BstDEI	CTNAG	6 cut(s)	476, 508, 705, 762, 960, 1620
BstDSI	CCRYGG	7 cut(s)	276, 289, 600, 1101, 1321, 1430, 1629
BstENI	CCTNNNNNAGG	1 cut(s)	1409
BstF5I	GGATG	2 cut(s)	1359, 1506
BstFNI	CGCG	5 cut(s)	114, 221, 291, 1203, 1631
BstH2I	RGCGCY	2 cut(s)	51, 483
BstHHI	GCGC	3 cut(s)	50, 221, 482
BstKTI	GATC	8 cut(s)	20, 241, 448, 463, 972, 1278, 1473, 1517
BstMAI	GTCTC	3 cut(s)	547, 1032, 1321
BstMBI	GATC	8 cut(s)	17, 238, 445, 460, 969, 1275, 1470, 1514
BstMCI	CGRYCG	2 cut(s)	225, 652
BstMWI	GCNNNNNNNGC	7 cut(s)	164, 207, 216, 221, 225, 680, 1625
BstNI	CCWGG	3 cut(s)	164, 377, 1301
BstPAI	GACNNNNGTC	2 cut(s)	932, 1454
BstSCI	CCNGG	4 cut(s)	162, 375, 1299, 1711
BstSFI	CTRYAG	2 cut(s)	669, 1186
BstSLI	GKGCMC	1 cut(s)	1692
BstUI	CGCG	5 cut(s)	114, 221, 291, 1203, 1631
BstV1I	GCAGC	3 cut(s)	655, 683, 1234
BstV2I	GAAGAC	1 cut(s)	572
BstX2I	RGATCY	2 cut(s)	17, 460
BstXI	CCANNNNNNTGG	1 cut(s)	1497
BstYI	RGATCY	2 cut(s)	17, 460
BstZI	CGGCCG	2 cut(s)	222, 649
Bsu15I	ATCGAT	2 cut(s)	390, 1473
BsuTUI	ATCGAT	2 cut(s)	390, 1473
BtgI	CCRYGG	7 cut(s)	276, 289, 600, 1101, 1321, 1430, 1629
BtgZI	GCGATG	1 cut(s)	555
BtrI	CACGTC	2 cut(s)	147, 1480
BtsCI	GGATG	2 cut(s)	1359, 1506
BtsI	GCAGTG	1 cut(s)	864
BtsIMutI	CAGTG	3 cut(s)	56, 108, 864
BveI	ACCTGC	2 cut(s)	206, 895
Cac8I	GCNNGC	8 cut(s)	176, 217, 226, 327, 440, 1306, 1626, 1702
CaiI	CAGNNNCTG	1 cut(s)	769
CfoI	GCGC	3 cut(s)	50, 221, 482
Cfr10I	RCCGGY	4 cut(s)	224, 530, 539, 974
Cfr13I	GGNCC	5 cut(s)	160, 334, 423, 953, 1449
Cfr42I	CCGCGG	2 cut(s)	292, 1632
ClaI	ATCGAT	2 cut(s)	390, 1473
CseI	GACGC	3 cut(s)	359, 545, 775
Csp6I	GTAC	6 cut(s)	382, 811, 999, 1159, 1174, 1441
CspAI	ACCGGT	2 cut(s)	530, 974
CviQI	GTAC	6 cut(s)	382, 811, 999, 1159, 1174, 1441
DdeI	CTNAG	6 cut(s)	476, 508, 705, 762, 960, 1620
DinI	GGCGCC	1 cut(s)	49
DpnI	GATC	8 cut(s)	19, 240, 447, 462, 971, 1277, 1472, 1516
DpnII	GATC	8 cut(s)	17, 238, 445, 460, 969, 1275, 1470, 1514
DriI	GACNNNNNGTC	1 cut(s)	121
EaeI	YGGCCR	6 cut(s)	133, 222, 327, 603, 649, 789
EagI	CGGCCG	2 cut(s)	222, 649
Eam1105I	GACNNNNNGTC	1 cut(s)	121
EciI	GGCGGA	1 cut(s)	21
Ecl136II	GAGCTC	2 cut(s)	373, 580
EclXI	CGGCCG	2 cut(s)	222, 649
Eco130I	CCWWGG	5 cut(s)	276, 330, 600, 1053, 1430
Eco147I	AGGCCT	1 cut(s)	1304
Eco24I	GRGCYC	3 cut(s)	375, 582, 634
Eco32I	GATATC	1 cut(s)	841
Eco47I	GGWCC	3 cut(s)	160, 334, 1449
Eco52I	CGGCCG	2 cut(s)	222, 649
Eco53kI	GAGCTC	2 cut(s)	373, 580
Eco88I	CYCGRG	4 cut(s)	337, 419, 1292, 1348
EcoICRI	GAGCTC	2 cut(s)	373, 580
EcoNI	CCTNNNNNAGG	1 cut(s)	1409
EcoO109I	RGGNCCY	2 cut(s)	334, 423
EcoRII	CCWGG	3 cut(s)	162, 375, 1299
EcoRV	GATATC	1 cut(s)	841
EcoT14I	CCWWGG	5 cut(s)	276, 330, 600, 1053, 1430
EcoT38I	GRGCYC	3 cut(s)	375, 582, 634
EgeI	GGCGCC	1 cut(s)	49
EheI	GGCGCC	1 cut(s)	49
ErhI	CCWWGG	5 cut(s)	276, 330, 600, 1053, 1430
FalI	AAGNNNNNCTT	2 cut(s)	1403, 1435
FaqI	GGGAC	1 cut(s)	1450
FauI	CCCGC	1 cut(s)	724
FbaI	TGATCA	1 cut(s)	969
Fnu4HI	GCNGC	9 cut(s)	112, 222, 483, 652, 669, 672, 1248, 1251, 1629
FokI	GGATG	2 cut(s)	1346, 1513
FriOI	GRGCYC	3 cut(s)	375, 582, 634
Fsp4HI	GCNGC	9 cut(s)	112, 222, 483, 652, 669, 672, 1248, 1251, 1629
FspBI	CTAG	3 cut(s)	1388, 1581, 1680
GlaI	GCGC	3 cut(s)	49, 220, 481
GluI	GCNGC	9 cut(s)	112, 222, 483, 652, 669, 672, 1248, 1251, 1629
GsuI	CTGGAG	3 cut(s)	341, 398, 1473
HaeII	RGCGCY	2 cut(s)	51, 483
HapII	CCGG	7 cut(s)	225, 531, 540, 648, 975, 1080, 1713
HgaI	GACGC	3 cut(s)	359, 545, 775
HhaI	GCGC	3 cut(s)	50, 221, 482
Hin1I	GRCGYC	2 cut(s)	48, 537
Hin6I	GCGC	3 cut(s)	48, 219, 480
HinP1I	GCGC	3 cut(s)	48, 219, 480
HincII	GTYRAC	1 cut(s)	823
HindII	GTYRAC	1 cut(s)	823
HindIII	AAGCTT	1 cut(s)	1610
HinfI	GANTC	6 cut(s)	61, 404, 1329, 1563, 1602, 1672
HpaII	CCGG	7 cut(s)	225, 531, 540, 648, 975, 1080, 1713
Hpy166II	GTNNAC	4 cut(s)	150, 823, 1176, 1385
Hpy188I	TCNGA	5 cut(s)	130, 412, 765, 947, 1123
Hpy188III	TCNNGA	8 cut(s)	301, 358, 562, 662, 1282, 1294, 1490, 1606
Hpy8I	GTNNAC	4 cut(s)	150, 823, 1176, 1385
Hpy99I	CGWCG	2 cut(s)	353, 1700
HpyAV	CCTTC	6 cut(s)	313, 729, 829, 856, 1487, 1578
HpyCH4III	ACNGT	7 cut(s)	91, 103, 558, 820, 967, 1024, 1464
HpyCH4IV	ACGT	7 cut(s)	146, 231, 314, 453, 1111, 1479, 1695
HpyF10VI	GCNNNNNNNGC	7 cut(s)	164, 207, 216, 221, 225, 680, 1625
HpyF3I	CTNAG	6 cut(s)	476, 508, 705, 762, 960, 1620
HpySE526I	ACGT	7 cut(s)	146, 231, 314, 453, 1111, 1479, 1695
Hsp92I	GRCGYC	2 cut(s)	48, 537
HspAI	GCGC	3 cut(s)	48, 219, 480
KasI	GGCGCC	1 cut(s)	47
KpnI	GGTACC	2 cut(s)	1002, 1162
KroI	GCCGGC	1 cut(s)	224
KroNI	GCCGGC	1 cut(s)	226
Ksp22I	TGATCA	1 cut(s)	969
KspI	CCGCGG	2 cut(s)	292, 1632
Kzo9I	GATC	8 cut(s)	17, 238, 445, 460, 969, 1275, 1470, 1514
LmnI	GCTCC	8 cut(s)	8, 291, 322, 370, 378, 901, 1082, 1492
Lsp1109I	GCAGC	3 cut(s)	655, 683, 1234
LweI	GCATC	2 cut(s)	1234, 1698
MaeI	CTAG	3 cut(s)	1388, 1581, 1680
MaeII	ACGT	7 cut(s)	146, 231, 314, 453, 1111, 1479, 1695
MaeIII	GTNAC	8 cut(s)	85, 97, 105, 232, 310, 401, 533, 1312
MalI	GATC	8 cut(s)	19, 240, 447, 462, 971, 1277, 1472, 1516
MboI	GATC	8 cut(s)	17, 238, 445, 460, 969, 1275, 1470, 1514
MboII	GAAGA	5 cut(s)	559, 577, 700, 1126, 1447
MflI	RGATCY	2 cut(s)	17, 460
MhlI	GDGCHC	5 cut(s)	344, 375, 582, 634, 1692
MlsI	TGGCCA	2 cut(s)	329, 791
MluCI	AATT	2 cut(s)	743, 1193
MluNI	TGGCCA	2 cut(s)	329, 791
Mly113I	GGCGCC	1 cut(s)	48
MlyI	GAGTC	3 cut(s)	398, 1323, 1557
Mox20I	TGGCCA	2 cut(s)	329, 791
MroNI	GCCGGC	1 cut(s)	224
MroXI	GAANNNNTTC	1 cut(s)	692
MscI	TGGCCA	2 cut(s)	329, 791
MseI	TTAA	3 cut(s)	636, 642, 1614
MslI	CAYNNNNRTG	1 cut(s)	1007
Msp20I	TGGCCA	2 cut(s)	329, 791
MspA1I	CMGCKG	3 cut(s)	291, 674, 1631
MspI	CCGG	7 cut(s)	225, 531, 540, 648, 975, 1080, 1713
MspR9I	CCNGG	4 cut(s)	164, 377, 1301, 1713
MvaI	CCWGG	3 cut(s)	164, 377, 1301
MvnI	CGCG	5 cut(s)	114, 221, 291, 1203, 1631
MwoI	GCNNNNNNNGC	7 cut(s)	164, 207, 216, 221, 225, 680, 1625
NaeI	GCCGGC	1 cut(s)	226
NarI	GGCGCC	1 cut(s)	48
NciI	CCSGG	1 cut(s)	1713
NcoI	CCATGG	3 cut(s)	276, 600, 1430
NdeII	GATC	8 cut(s)	17, 238, 445, 460, 969, 1275, 1470, 1514
NgoMIV	GCCGGC	1 cut(s)	224
NmuCI	GTSAC	7 cut(s)	97, 105, 232, 310, 401, 533, 1312
PaeR7I	CTCGAG	1 cut(s)	1292
PceI	AGGCCT	1 cut(s)	1304
PcsI	WCGNNNNNNNCGW	3 cut(s)	306, 450, 1329
PdiI	GCCGGC	1 cut(s)	226
PdmI	GAANNNNTTC	1 cut(s)	692
PfeI	GAWTC	3 cut(s)	61, 1602, 1672
PflMI	CCANNNNNTGG	1 cut(s)	1102
PfoI	TCCNGGA	1 cut(s)	375
PinAI	ACCGGT	2 cut(s)	530, 974
PkrI	GCNGC	9 cut(s)	113, 223, 484, 653, 670, 673, 1249, 1252, 1630
PleI	GAGTC	3 cut(s)	398, 1323, 1557
PluTI	GGCGCC	1 cut(s)	51
PpsI	GAGTC	3 cut(s)	398, 1323, 1557
Ppu21I	YACGTR	2 cut(s)	454, 1112
PpuMI	RGGWCCY	1 cut(s)	334
PshAI	GACNNNNGTC	2 cut(s)	932, 1454
Psp124BI	GAGCTC	2 cut(s)	375, 582
Psp5II	RGGWCCY	1 cut(s)	334
Psp6I	CCWGG	3 cut(s)	162, 375, 1299
PspGI	CCWGG	3 cut(s)	162, 375, 1299
PspPI	GGNCC	5 cut(s)	160, 334, 423, 953, 1449
PspPPI	RGGWCCY	1 cut(s)	334
PstI	CTGCAG	1 cut(s)	673
PstNI	CAGNNNCTG	1 cut(s)	769
PsuI	RGATCY	2 cut(s)	17, 460
PvuII	CAGCTG	1 cut(s)	674
RsaI	GTAC	6 cut(s)	383, 812, 1000, 1160, 1175, 1442
RsaNI	GTAC	6 cut(s)	382, 811, 999, 1159, 1174, 1441
RseI	CAYNNNNRTG	1 cut(s)	1007
SacI	GAGCTC	2 cut(s)	375, 582
SacII	CCGCGG	2 cut(s)	292, 1632
SaqAI	TTAA	3 cut(s)	636, 642, 1614
SatI	GCNGC	9 cut(s)	112, 222, 483, 652, 669, 672, 1248, 1251, 1629
Sau3AI	GATC	8 cut(s)	17, 238, 445, 460, 969, 1275, 1470, 1514
Sau96I	GGNCC	5 cut(s)	160, 334, 423, 953, 1449
SchI	GAGTC	3 cut(s)	398, 1323, 1557
ScrFI	CCNGG	4 cut(s)	164, 377, 1301, 1713
SduI	GDGCHC	5 cut(s)	344, 375, 582, 634, 1692
SfaNI	GCATC	2 cut(s)	1234, 1698
SfcI	CTRYAG	2 cut(s)	669, 1186
SfoI	GGCGCC	1 cut(s)	49
Sfr274I	CTCGAG	1 cut(s)	1292
Sfr303I	CCGCGG	2 cut(s)	292, 1632
SgrAI	CRCCGGYG	2 cut(s)	530, 539
SgrBI	CCGCGG	2 cut(s)	292, 1632
SinI	GGWCC	3 cut(s)	160, 334, 1449
SlaI	CTCGAG	1 cut(s)	1292
SmiMI	CAYNNNNRTG	1 cut(s)	1007
SmlI	CTYRAG	3 cut(s)	464, 662, 1292
SmoI	CTYRAG	3 cut(s)	464, 662, 1292
Sse9I	AATT	2 cut(s)	743, 1193
SseBI	AGGCCT	1 cut(s)	1304
SspDI	GGCGCC	1 cut(s)	47
SspMI	CTAG	3 cut(s)	1388, 1581, 1680
SstI	GAGCTC	2 cut(s)	375, 582
StuI	AGGCCT	1 cut(s)	1304
StyD4I	CCNGG	4 cut(s)	162, 375, 1299, 1711
StyI	CCWWGG	5 cut(s)	276, 330, 600, 1053, 1430
TaaI	ACNGT	7 cut(s)	91, 103, 558, 820, 967, 1024, 1464
TaiI	ACGT	7 cut(s)	149, 234, 317, 456, 1114, 1482, 1698
TaqI	TCGA	7 cut(s)	300, 390, 444, 1227, 1293, 1332, 1473
TasI	AATT	2 cut(s)	743, 1193
TatI	WGTACW	2 cut(s)	810, 1173
TauI	GCSGC	6 cut(s)	114, 224, 485, 654, 1253, 1631
TfiI	GAWTC	3 cut(s)	61, 1602, 1672
Tru1I	TTAA	3 cut(s)	636, 642, 1614
Tru9I	TTAA	3 cut(s)	636, 642, 1614
TscAI	CASTG	3 cut(s)	63, 108, 864
TseFI	GTSAC	7 cut(s)	97, 105, 232, 310, 401, 533, 1312
TseI	GCWGC	3 cut(s)	668, 671, 1247
Tsp45I	GTSAC	7 cut(s)	97, 105, 232, 310, 401, 533, 1312
TspDTI	ATGAA	2 cut(s)	1036, 1222
TspGWI	ACGGA	2 cut(s)	30, 298
TspRI	CASTG	3 cut(s)	63, 108, 864
Van91I	CCANNNNNTGG	1 cut(s)	1102
VpaK11BI	GGWCC	3 cut(s)	160, 334, 1449
XagI	CCTNNNNNAGG	1 cut(s)	1409
XhoI	CTCGAG	1 cut(s)	1292
XmnI	GAANNNNTTC	1 cut(s)	692
XspI	CTAG	3 cut(s)	1388, 1581, 1680

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.