Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Forward (+)

13019403 .. 13021896

Gene Structure 2494 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1164800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1737 bp

ATGAACGCTGAACATAATAAGAAGAAGAAGTTCGCCATCATCGGCGTTTCATCTGTGATTCTAGTAGCCATGGTGGTTGCCGTAACGGTTGGGGTAACCACAAAAAACAAAGTAGGGGGAGGGCACAAAAACTCGGACGGCGGGCATGTCACCACGTCGACCAAAGCAATAAAGGCTATATGCCAGCCGACGGATTATAAGGAAACCTGCGAGAGGAGTCTGGAATCGGCTGCTGGCAACAGCACGGAGCCGAAAGACCTCATCAAGGCAGGTTTCAACGTTACCATGGACAAACTTCGGGCCATCATAAAAAACTCCACCACATTGCAAGAACTGGCCAAGGACGAAAGTACAAACCAAGCTCTAGAAAATTGCAAGGAGCTCTTGGAGTATGCCATCGACGATCTCACGACATCTTTCCAAAAATTGGGTCCTTTCGACATCAGCAAGATCGATGATTACGCGGAAGATCTCAAGGTCTGGCTCAGCGCGGCTATTACTTACCAACAAACATGCTTGGACGGGTTTCAGAACACCAAAGGCGACGCCGGCGACAAGATGAAGCGTTTCTTGAACACCACGCAACAGCTCACCAGCAACGGACTTGCCATGGTGACCGAAATTACTTCCGTGCTTGGGTCTCTCAATTTGAAAGCAAACCGGCGTCGTTTGCTGGCAGGGGGAGGGGGTGGAGGTAATGCGAAGAATGCTCCAAAGGTTATTCCTACGTGGATCAACAATAAGCGTAGCCTCGACGTGTCTACCGTGACTCCACAGTCGATTAAACCGGACGCAGTGGTGGCTAAGGATGGGAGTGGACAGTACGAGACTATTGGTGAAGCTGTGAAGATGATCCCGAAGAACAATGCGGACAAGACGTTTGTGATTTATGTGAAGGAGGGAGTGTACAGTGAGTATGTCATGATTGACAAGTATATGACCAACGTCATGTTGATTGGGGATGGCGCTACTAAGACCAAGGTCACCGGTAGCAAAAACTTCGCTGCGGGAGTCCAAACTTTTCAGACTGCAACAGTCGTTGTAGTTGGGGACTACTTCATTGCTAAGGACATAGGGATTGAGAACTCAGCAGGAGCCGAAGGGCACCAAGCCGTGGCTCTGCGAGTGCAGTCGGACTTGTCCATCTTCTACCGCTGCCAAATGGACGGGTACCAAGACACTCTCTACACTCAAACCCACCGGCAATTCTACCGCGACTGCACCATCAGCGGCACAATCGACTTTATCTTTGGAGACGCTGCCGTAGTGTTCCAGAACTGCAAGATGATCGTAAGAAAACCAATGGACAACCAAGCTTGCATGGTCACAGCCCAAGGGAGAATTGACCGGCGTCAGCCCTCGGGTATCATCCTCCAAAACTGCACCATCTCCGGGGACCCGGAGTACATTCCCGTGAAGGACAAGAACAAGTCGTACCTGGGGAGGCCGTGGAAGAACCTAGCTAGGACCGTCGTGATGCAGTCGCAGATTGATGGCATCATTGCTCCGGAAGGGTGGATGGAGTGGACGGGTTCGAACAATCATCAAAGTTGCTGGTTTGGCGAATACAGAAACAGAGGGCCTGGCTCCCACATGAGCAAGAGGGTGAAGTGGAGCGGCATCAAGAATCTTAGTGCCGACCAGGCTGTCGCTTTCACCTCCGGTAAGTTTGTTGAGGGTGATAAGTGGATCAAGCCCAGCGGTGTGCCTTACGTTGCTGGCATGGTTTCCGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

579

Amino Acids

63.15

Weight (kDa)

8.94

Isoelectric Point (pI)

32.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	55 - 204	3.8e-36	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	264 - 561	1.5e-134	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	198
AasI	GACNNNNNNGTC	2 cut(s)	775, 1646
Acc36I	ACCTGC	2 cut(s)	215, 260
Acc65I	GGTACC	1 cut(s)	1170
AccB1I	GGYRCC	2 cut(s)	1104, 1170
AccB7I	CCANNNNNTGG	2 cut(s)	427, 1114
AccBSI	CCGCTC	1 cut(s)	1617
AccI	GTMKAC	2 cut(s)	158, 761
AccII	CGCG	3 cut(s)	464, 491, 1215
AccIII	TCCGGA	1 cut(s)	1507
AclI	AACGTT	1 cut(s)	279
AclWI	GGATC	3 cut(s)	740, 847, 1697
AcoI	YGGCCR	1 cut(s)	336
AcyI	GRCGYC	3 cut(s)	546, 664, 1351
AfaI	GTAC	6 cut(s)	352, 824, 908, 1172, 1406, 1436
AfiI	CCNNNNNNNGG	7 cut(s)	190, 213, 265, 427, 636, 1114, 1392
AflIII	ACRYGT	1 cut(s)	756
AgeI	ACCGGT	1 cut(s)	986
AgsI	TTSAA	3 cut(s)	277, 574, 652
AjiI	CACGTC	2 cut(s)	156, 757
AjnI	CCWGG	3 cut(s)	1437, 1582, 1641
AluBI	AGCT	6 cut(s)	362, 382, 589, 842, 1316, 1463
AluI	AGCT	6 cut(s)	362, 382, 589, 842, 1316, 1463
Alw21I	GWGCWC	1 cut(s)	384
Alw26I	GTCTC	3 cut(s)	645, 821, 1248
AlwI	GGATC	3 cut(s)	740, 847, 1697
Ama87I	CYCGRG	1 cut(s)	1360
Aor13HI	TCCGGA	1 cut(s)	1507
AoxI	GGCC	4 cut(s)	300, 336, 1445, 1580
ApeKI	GCWGC	4 cut(s)	230, 1004, 1155, 1259
ArsI	GACNNNNNNTTYG	6 cut(s)	415, 447, 863, 895, 1232, 1264
AsiGI	ACCGGT	1 cut(s)	986
Asp700I	GAANNNNTTC	2 cut(s)	29, 566
Asp718I	GGTACC	1 cut(s)	1170
AspLEI	GCGC	2 cut(s)	491, 968
AspS9I	GGNCC	5 cut(s)	300, 431, 1396, 1467, 1580
AsuC2I	CCSGG	2 cut(s)	1393, 1400
AsuHPI	GGTGA	8 cut(s)	142, 583, 625, 848, 976, 1618, 1648, 1691
AsuII	TTCGAA	1 cut(s)	1535
AvaI	CYCGRG	1 cut(s)	1360
AvaII	GGWCC	3 cut(s)	431, 1396, 1467
BaeGI	GKGCMC	2 cut(s)	126, 1107
BalI	TGGCCA	1 cut(s)	338
BanI	GGYRCC	2 cut(s)	1104, 1170
BanII	GRGCYC	1 cut(s)	384
Bbv12I	GWGCWC	1 cut(s)	384
BbvI	GCAGC	4 cut(s)	217, 991, 1142, 1246
BceAI	ACGGC	5 cut(s)	65, 154, 1097, 1247, 1432
BcgI	CGANNNNNNTGC	4 cut(s)	982, 1016, 1270, 1304
BciT130I	CCWGG	3 cut(s)	1439, 1584, 1643
BcnI	CCSGG	2 cut(s)	1393, 1400
BcoDI	GTCTC	3 cut(s)	645, 821, 1248
BfaI	CTAG	4 cut(s)	62, 365, 1460, 1464
BfoI	RGCGCY	1 cut(s)	969
BfuAI	ACCTGC	2 cut(s)	215, 260
BglI	GCCNNNNNGGC	1 cut(s)	1643
BglII	AGATCT	1 cut(s)	469
BisI	GCNGC	7 cut(s)	231, 492, 1005, 1156, 1231, 1260, 1618
BlpI	GCTNAGC	1 cut(s)	485
BlsI	GCNGC	7 cut(s)	232, 493, 1006, 1157, 1232, 1261, 1619
Bme1390I	CCNGG	5 cut(s)	1393, 1400, 1439, 1584, 1643
Bme18I	GGWCC	3 cut(s)	431, 1396, 1467
BmeT110I	CYCGRG	1 cut(s)	1360
BmgBI	CACGTC	2 cut(s)	156, 757
BmgT120I	GGNCC	5 cut(s)	300, 431, 1396, 1467, 1580
BmiI	GGNNCC	8 cut(s)	249, 432, 1096, 1106, 1172, 1397, 1398, 1588
BmrFI	CCNGG	5 cut(s)	1393, 1400, 1439, 1584, 1643
BmsI	GCATC	3 cut(s)	1467, 1506, 1629
BoxI	GACNNNNGTC	3 cut(s)	944, 980, 1350
Bpu10I	CCTNAGC	2 cut(s)	804, 1065
Bpu1102I	GCTNAGC	1 cut(s)	485
Bpu14I	TTCGAA	1 cut(s)	1535
BpuEI	CTTGAG	1 cut(s)	458
BpuMI	CCSGG	2 cut(s)	1393, 1400
Bsa29I	ATCGAT	1 cut(s)	453
BsaAI	YACGTR	1 cut(s)	729
BsaHI	GRCGYC	3 cut(s)	546, 664, 1351
BsaI	GGTCTC	1 cut(s)	645
BsaWI	WCCGGW	4 cut(s)	787, 986, 1507, 1661
BsaXI	ACNNNNNCTCC	4 cut(s)	754, 784, 890, 920
Bsc4I	CCNNNNNNNGG	7 cut(s)	190, 213, 265, 427, 636, 1114, 1392
Bse118I	RCCGGY	5 cut(s)	548, 660, 986, 1200, 1347
Bse1I	ACTGG	1 cut(s)	339
Bse3DI	GCAATG	3 cut(s)	323, 1059, 1500
BseAI	TCCGGA	1 cut(s)	1507
BseBI	CCWGG	3 cut(s)	1439, 1584, 1643
BseCI	ATCGAT	1 cut(s)	453
BseGI	GGATG	4 cut(s)	814, 967, 1368, 1524
BseLI	CCNNNNNNNGG	7 cut(s)	190, 213, 265, 427, 636, 1114, 1392
BseMI	GCAATG	3 cut(s)	323, 1059, 1500
BseMII	CTCAG	2 cut(s)	499, 1101
BseNI	ACTGG	1 cut(s)	339
BseRI	GAGGAG	1 cut(s)	229
BseSI	GKGCMC	2 cut(s)	126, 1107
BseXI	GCAGC	4 cut(s)	217, 991, 1142, 1246
BseYI	CCCAGC	1 cut(s)	1697
BsgI	GTGCAG	3 cut(s)	1148, 1204, 1366
Bsh1236I	CGCG	3 cut(s)	464, 491, 1215
BshFI	GGCC	4 cut(s)	302, 338, 1447, 1582
BshNI	GGYRCC	2 cut(s)	1104, 1170
BshTI	ACCGGT	1 cut(s)	986
BshVI	ATCGAT	1 cut(s)	453
BsiHKAI	GWGCWC	1 cut(s)	384
BsiHKCI	CYCGRG	1 cut(s)	1360
BslFI	GGGAC	2 cut(s)	1064, 1409
BslI	CCNNNNNNNGG	7 cut(s)	190, 213, 265, 427, 636, 1114, 1392
BsmAI	GTCTC	3 cut(s)	645, 821, 1248
BsmBI	CGTCTC	1 cut(s)	1248
BsmFI	GGGAC	2 cut(s)	1064, 1409
BsmI	GAATGC	1 cut(s)	712
BsnI	GGCC	4 cut(s)	302, 338, 1447, 1582
Bso31I	GGTCTC	1 cut(s)	645
BsoBI	CYCGRG	1 cut(s)	1360
Bsp119I	TTCGAA	1 cut(s)	1535
Bsp1286I	GDGCHC	3 cut(s)	126, 384, 1107
Bsp13I	TCCGGA	1 cut(s)	1507
Bsp1407I	TGTACA	1 cut(s)	906
Bsp143I	GATC	7 cut(s)	403, 450, 469, 732, 852, 1287, 1689
Bsp1720I	GCTNAGC	1 cut(s)	485
Bsp19I	CCATGG	3 cut(s)	69, 285, 609
BspANI	GGCC	4 cut(s)	302, 338, 1447, 1582
BspCNI	CTCAG	2 cut(s)	498, 1100
BspDI	ATCGAT	1 cut(s)	453
BspEI	TCCGGA	1 cut(s)	1507
BspFNI	CGCG	3 cut(s)	464, 491, 1215
BspHI	TCATGA	1 cut(s)	921
BspLI	GGNNCC	8 cut(s)	249, 432, 1096, 1106, 1172, 1397, 1398, 1588
BspMI	ACCTGC	2 cut(s)	215, 260
BspPI	GGATC	3 cut(s)	740, 847, 1697
BspT104I	TTCGAA	1 cut(s)	1535
BspT107I	GGYRCC	2 cut(s)	1104, 1170
BspTNI	GGTCTC	1 cut(s)	645
BsrBI	CCGCTC	1 cut(s)	1617
BsrDI	GCAATG	3 cut(s)	323, 1059, 1500
BsrFI	RCCGGY	5 cut(s)	548, 660, 986, 1200, 1347
BsrGI	TGTACA	1 cut(s)	906
BsrI	ACTGG	1 cut(s)	339
BssAI	RCCGGY	5 cut(s)	548, 660, 986, 1200, 1347
BssMI	GATC	7 cut(s)	403, 450, 469, 732, 852, 1287, 1689
BssNI	GRCGYC	3 cut(s)	546, 664, 1351
BssT1I	CCWWGG	6 cut(s)	69, 285, 339, 609, 978, 1333
Bst2UI	CCWGG	3 cut(s)	1439, 1584, 1643
Bst4CI	ACNGT	7 cut(s)	88, 766, 777, 822, 911, 1036, 1471
BstACI	GRCGYC	3 cut(s)	546, 664, 1351
BstAUI	TGTACA	1 cut(s)	906
BstBAI	YACGTR	1 cut(s)	729
BstBI	TTCGAA	1 cut(s)	1535
BstC8I	GCNNGC	7 cut(s)	143, 185, 235, 550, 675, 1318, 1720
BstDEI	CTNAG	6 cut(s)	485, 804, 972, 1065, 1087, 1631
BstDSI	CCRYGG	5 cut(s)	69, 285, 609, 1113, 1448
BstEII	GGTNACC	3 cut(s)	94, 613, 982
BstENI	CCTNNNNNAGG	2 cut(s)	211, 263
BstF5I	GGATG	4 cut(s)	814, 967, 1368, 1524
BstFNI	CGCG	3 cut(s)	464, 491, 1215
BstH2I	RGCGCY	1 cut(s)	969
BstHHI	GCGC	2 cut(s)	491, 968
BstKTI	GATC	7 cut(s)	406, 453, 472, 735, 855, 1290, 1692
BstMAI	GTCTC	3 cut(s)	645, 821, 1248
BstMBI	GATC	7 cut(s)	403, 450, 469, 732, 852, 1287, 1689
BstMWI	GCNNNNNNNGC	8 cut(s)	173, 549, 670, 707, 800, 1227, 1560, 1643
BstNI	CCWGG	3 cut(s)	1439, 1584, 1643
BstNSI	RCATGY	2 cut(s)	149, 516
BstPAI	GACNNNNGTC	3 cut(s)	944, 980, 1350
BstPI	GGTNACC	3 cut(s)	94, 613, 982
BstSCI	CCNGG	5 cut(s)	1391, 1398, 1437, 1582, 1641
BstSLI	GKGCMC	2 cut(s)	126, 1107
BstUI	CGCG	3 cut(s)	464, 491, 1215
BstV1I	GCAGC	4 cut(s)	217, 991, 1142, 1246
BstX2I	RGATCY	1 cut(s)	469
BstYI	RGATCY	1 cut(s)	469
Bsu15I	ATCGAT	1 cut(s)	453
BsuRI	GGCC	4 cut(s)	302, 338, 1447, 1582
BsuTUI	ATCGAT	1 cut(s)	453
BtgI	CCRYGG	5 cut(s)	69, 285, 609, 1113, 1448
BtrI	CACGTC	2 cut(s)	156, 757
BtsCI	GGATG	4 cut(s)	814, 967, 1368, 1524
BtsI	GCAGTG	1 cut(s)	801
BtsIMutI	CAGTG	2 cut(s)	801, 916
BveI	ACCTGC	2 cut(s)	215, 260
Cac8I	GCNNGC	7 cut(s)	143, 185, 235, 550, 675, 1318, 1720
CciI	TCATGA	1 cut(s)	921
CfoI	GCGC	2 cut(s)	491, 968
Cfr10I	RCCGGY	5 cut(s)	548, 660, 986, 1200, 1347
Cfr13I	GGNCC	5 cut(s)	300, 431, 1396, 1467, 1580
ClaI	ATCGAT	1 cut(s)	453
CseI	GACGC	5 cut(s)	554, 653, 800, 1265, 1340
Csp6I	GTAC	6 cut(s)	351, 823, 907, 1171, 1405, 1435
CspAI	ACCGGT	1 cut(s)	986
CviQI	GTAC	6 cut(s)	351, 823, 907, 1171, 1405, 1435
DdeI	CTNAG	6 cut(s)	485, 804, 972, 1065, 1087, 1631
DpnI	GATC	7 cut(s)	405, 452, 471, 734, 854, 1289, 1691
DpnII	GATC	7 cut(s)	403, 450, 469, 732, 852, 1287, 1689
DrdI	GACNNNNNNGTC	2 cut(s)	775, 1646
DseDI	GACNNNNNNGTC	2 cut(s)	775, 1646
EaeI	YGGCCR	1 cut(s)	336
Ecl136II	GAGCTC	1 cut(s)	382
Eco130I	CCWWGG	6 cut(s)	69, 285, 339, 609, 978, 1333
Eco24I	GRGCYC	1 cut(s)	384
Eco31I	GGTCTC	1 cut(s)	645
Eco47I	GGWCC	3 cut(s)	431, 1396, 1467
Eco53kI	GAGCTC	1 cut(s)	382
Eco88I	CYCGRG	1 cut(s)	1360
Eco91I	GGTNACC	3 cut(s)	94, 613, 982
EcoICRI	GAGCTC	1 cut(s)	382
EcoNI	CCTNNNNNAGG	2 cut(s)	211, 263
EcoO109I	RGGNCCY	3 cut(s)	431, 1396, 1580
EcoO65I	GGTNACC	3 cut(s)	94, 613, 982
EcoRII	CCWGG	3 cut(s)	1437, 1582, 1641
EcoT14I	CCWWGG	6 cut(s)	69, 285, 339, 609, 978, 1333
EcoT38I	GRGCYC	1 cut(s)	384
ErhI	CCWWGG	6 cut(s)	69, 285, 339, 609, 978, 1333
Esp3I	CGTCTC	1 cut(s)	1248
FalI	AAGNNNNNCTT	4 cut(s)	368, 400, 554, 586
FaqI	GGGAC	2 cut(s)	1064, 1409
FauI	CCCGC	2 cut(s)	134, 1000
FblI	GTMKAC	2 cut(s)	158, 761
Fnu4HI	GCNGC	7 cut(s)	231, 492, 1005, 1156, 1231, 1260, 1618
FokI	GGATG	4 cut(s)	821, 974, 1355, 1531
FriOI	GRGCYC	1 cut(s)	384
Fsp4HI	GCNGC	7 cut(s)	231, 492, 1005, 1156, 1231, 1260, 1618
FspBI	CTAG	4 cut(s)	62, 365, 1460, 1464
GlaI	GCGC	2 cut(s)	490, 967
GluI	GCNGC	7 cut(s)	231, 492, 1005, 1156, 1231, 1260, 1618
GsaI	CCCAGC	1 cut(s)	1701
HaeII	RGCGCY	1 cut(s)	969
HaeIII	GGCC	4 cut(s)	302, 338, 1447, 1582
HgaI	GACGC	5 cut(s)	554, 653, 800, 1265, 1340
HhaI	GCGC	2 cut(s)	491, 968
Hin1I	GRCGYC	3 cut(s)	546, 664, 1351
Hin6I	GCGC	2 cut(s)	489, 966
HinP1I	GCGC	2 cut(s)	489, 966
HincII	GTYRAC	1 cut(s)	159
HindII	GTYRAC	1 cut(s)	159
HindIII	AAGCTT	1 cut(s)	1314
HinfI	GANTC	6 cut(s)	58, 217, 224, 769, 1011, 1627
HphI	GGTGA	8 cut(s)	142, 583, 625, 848, 976, 1618, 1648, 1691
Hpy166II	GTNNAC	5 cut(s)	159, 762, 818, 907, 1527
Hpy188I	TCNGA	4 cut(s)	136, 531, 1026, 1135
Hpy8I	GTNNAC	5 cut(s)	159, 762, 818, 907, 1527
Hpy99I	CGWCG	7 cut(s)	160, 193, 404, 548, 669, 758, 1475
HpyAV	CCTTC	4 cut(s)	889, 1094, 1411, 1505
HpyCH4III	ACNGT	7 cut(s)	88, 766, 777, 822, 911, 1036, 1471
HpyCH4IV	ACGT	7 cut(s)	155, 279, 728, 756, 878, 945, 1713
HpyCH4V	TGCA	9 cut(s)	328, 375, 1031, 1129, 1221, 1281, 1320, 1383, 1480
HpyF10VI	GCNNNNNNNGC	8 cut(s)	173, 549, 670, 707, 800, 1227, 1560, 1643
HpyF3I	CTNAG	6 cut(s)	485, 804, 972, 1065, 1087, 1631
HpySE526I	ACGT	7 cut(s)	155, 279, 728, 756, 878, 945, 1713
Hsp92I	GRCGYC	3 cut(s)	546, 664, 1351
HspAI	GCGC	2 cut(s)	489, 966
KflI	GGGWCCC	1 cut(s)	1396
Kpn2I	TCCGGA	1 cut(s)	1507
KpnI	GGTACC	1 cut(s)	1174
KroI	GCCGGC	1 cut(s)	548
KroNI	GCCGGC	1 cut(s)	550
Kzo9I	GATC	7 cut(s)	403, 450, 469, 732, 852, 1287, 1689
LmnI	GCTCC	7 cut(s)	247, 379, 715, 1094, 1510, 1592, 1614
Lsp1109I	GCAGC	4 cut(s)	217, 991, 1142, 1246
LweI	GCATC	3 cut(s)	1467, 1506, 1629
MaeI	CTAG	4 cut(s)	62, 365, 1460, 1464
MaeII	ACGT	7 cut(s)	155, 279, 728, 756, 878, 945, 1713
MaeIII	GTNAC	8 cut(s)	82, 94, 148, 280, 613, 766, 982, 1324
MalI	GATC	7 cut(s)	405, 452, 471, 734, 854, 1289, 1691
MbiI	CCGCTC	1 cut(s)	1617
MboI	GATC	7 cut(s)	403, 450, 469, 732, 852, 1287, 1689
MboII	GAAGA	8 cut(s)	34, 37, 479, 715, 859, 871, 1138, 1465
MflI	RGATCY	1 cut(s)	469
MhlI	GDGCHC	3 cut(s)	126, 384, 1107
MlsI	TGGCCA	1 cut(s)	338
MluCI	AATT	6 cut(s)	370, 425, 621, 646, 1205, 1341
MluNI	TGGCCA	1 cut(s)	338
MlyI	GAGTC	3 cut(s)	226, 763, 1020
MmeI	TCCRAC	1 cut(s)	1113
Mox20I	TGGCCA	1 cut(s)	338
MreI	CGCCGGCG	1 cut(s)	548
MroI	TCCGGA	1 cut(s)	1507
MroNI	GCCGGC	1 cut(s)	548
MroXI	GAANNNNTTC	2 cut(s)	29, 566
MscI	TGGCCA	1 cut(s)	338
MseI	TTAA	1 cut(s)	783
MslI	CAYNNNNRTG	1 cut(s)	1412
Msp20I	TGGCCA	1 cut(s)	338
MspA1I	CMGCKG	3 cut(s)	1155, 1230, 1701
MspR9I	CCNGG	5 cut(s)	1393, 1400, 1439, 1584, 1643
Mva1269I	GAATGC	1 cut(s)	712
MvaI	CCWGG	3 cut(s)	1439, 1584, 1643
MvnI	CGCG	3 cut(s)	464, 491, 1215
MwoI	GCNNNNNNNGC	8 cut(s)	173, 549, 670, 707, 800, 1227, 1560, 1643
NaeI	GCCGGC	1 cut(s)	550
NciI	CCSGG	2 cut(s)	1393, 1400
NcoI	CCATGG	3 cut(s)	69, 285, 609
NdeII	GATC	7 cut(s)	403, 450, 469, 732, 852, 1287, 1689
NgoMIV	GCCGGC	1 cut(s)	548
NlaIV	GGNNCC	8 cut(s)	249, 432, 1096, 1106, 1172, 1397, 1398, 1588
NmuCI	GTSAC	5 cut(s)	148, 613, 766, 982, 1324
NspI	RCATGY	2 cut(s)	149, 516
NspV	TTCGAA	1 cut(s)	1535
PagI	TCATGA	1 cut(s)	921
PcsI	WCGNNNNNNNCGW	1 cut(s)	762
PctI	GAATGC	1 cut(s)	712
PdiI	GCCGGC	1 cut(s)	550
PdmI	GAANNNNTTC	2 cut(s)	29, 566
PfeI	GAWTC	3 cut(s)	58, 224, 1627
PflMI	CCANNNNNTGG	2 cut(s)	427, 1114
PinAI	ACCGGT	1 cut(s)	986
PkrI	GCNGC	7 cut(s)	232, 493, 1006, 1157, 1232, 1261, 1619
PleI	GAGTC	3 cut(s)	225, 763, 1019
PpsI	GAGTC	3 cut(s)	225, 763, 1019
Ppu21I	YACGTR	1 cut(s)	729
PpuMI	RGGWCCY	2 cut(s)	431, 1396
PshAI	GACNNNNGTC	3 cut(s)	944, 980, 1350
PsiI	TTATAA	1 cut(s)	198
Psp124BI	GAGCTC	1 cut(s)	384
Psp1406I	AACGTT	1 cut(s)	279
Psp5II	RGGWCCY	2 cut(s)	431, 1396
Psp6I	CCWGG	3 cut(s)	1437, 1582, 1641
PspEI	GGTNACC	3 cut(s)	94, 613, 982
PspFI	CCCAGC	1 cut(s)	1697
PspGI	CCWGG	3 cut(s)	1437, 1582, 1641
PspN4I	GGNNCC	8 cut(s)	249, 432, 1096, 1106, 1172, 1397, 1398, 1588
PspPI	GGNCC	5 cut(s)	300, 431, 1396, 1467, 1580
PspPPI	RGGWCCY	2 cut(s)	431, 1396
PsrI	GAACNNNNNNTAC	2 cut(s)	1418, 1450
PsuI	RGATCY	1 cut(s)	469
RsaI	GTAC	6 cut(s)	352, 824, 908, 1172, 1406, 1436
RsaNI	GTAC	6 cut(s)	351, 823, 907, 1171, 1405, 1435
RseI	CAYNNNNRTG	1 cut(s)	1412
SacI	GAGCTC	1 cut(s)	384
SalI	GTCGAC	1 cut(s)	157
SaqAI	TTAA	1 cut(s)	783
SatI	GCNGC	7 cut(s)	231, 492, 1005, 1156, 1231, 1260, 1618
Sau3AI	GATC	7 cut(s)	403, 450, 469, 732, 852, 1287, 1689
Sau96I	GGNCC	5 cut(s)	300, 431, 1396, 1467, 1580
SchI	GAGTC	3 cut(s)	226, 763, 1020
ScrFI	CCNGG	5 cut(s)	1393, 1400, 1439, 1584, 1643
SduI	GDGCHC	3 cut(s)	126, 384, 1107
SfaNI	GCATC	3 cut(s)	1467, 1506, 1629
SfuI	TTCGAA	1 cut(s)	1535
SgrAI	CRCCGGYG	1 cut(s)	548
SinI	GGWCC	3 cut(s)	431, 1396, 1467
SmiMI	CAYNNNNRTG	1 cut(s)	1412
SmlI	CTYRAG	1 cut(s)	473
SmoI	CTYRAG	1 cut(s)	473
Sse9I	AATT	6 cut(s)	370, 425, 621, 646, 1205, 1341
SspMI	CTAG	4 cut(s)	62, 365, 1460, 1464
SstI	GAGCTC	1 cut(s)	384
StyD4I	CCNGG	5 cut(s)	1391, 1398, 1437, 1582, 1641
StyI	CCWWGG	6 cut(s)	69, 285, 339, 609, 978, 1333
TaaI	ACNGT	7 cut(s)	88, 766, 777, 822, 911, 1036, 1471
TaiI	ACGT	7 cut(s)	158, 282, 731, 759, 881, 948, 1716
TaqI	TCGA	8 cut(s)	158, 399, 438, 453, 753, 779, 1239, 1535
TaqII	GACCGA	1 cut(s)	632
TasI	AATT	6 cut(s)	370, 425, 621, 646, 1205, 1341
TatI	WGTACW	3 cut(s)	350, 906, 1404
TauI	GCSGC	3 cut(s)	494, 1233, 1620
TfiI	GAWTC	3 cut(s)	58, 224, 1627
Tru1I	TTAA	1 cut(s)	783
Tru9I	TTAA	1 cut(s)	783
TscAI	CASTG	2 cut(s)	801, 916
TseFI	GTSAC	5 cut(s)	148, 613, 766, 982, 1324
TseI	GCWGC	4 cut(s)	230, 1004, 1155, 1259
Tsp45I	GTSAC	5 cut(s)	148, 613, 766, 982, 1324
TspDTI	ATGAA	4 cut(s)	17, 39, 575, 1048
TspGWI	ACGGA	5 cut(s)	206, 260, 615, 619, 1720
TspRI	CASTG	2 cut(s)	801, 916
Van91I	CCANNNNNTGG	2 cut(s)	427, 1114
VpaK11BI	GGWCC	3 cut(s)	431, 1396, 1467
XagI	CCTNNNNNAGG	2 cut(s)	211, 263
XbaI	TCTAGA	1 cut(s)	364
XceI	RCATGY	2 cut(s)	149, 516
XmiI	GTMKAC	2 cut(s)	158, 761
XmnI	GAANNNNTTC	2 cut(s)	29, 566
XspI	CTAG	4 cut(s)	62, 365, 1460, 1464

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.