Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Reverse (-)

5850366 .. 5851296

Gene Structure 931 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG074300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 798 bp

ATGACCAATCTGATGCTGATCGGAGACGGTGCTAATAAGACCAGAATCACCGGAAACTTGAACTTCGTAGACGGCACCCCTACCTACAAAACCGCTACAGTTGCGGTCCATGGAGCTCACTTCATGGCTAGGGATATTGGGTTTGAGAACTCAGCCGGTCCAGACAAACATCAAGCTGTGGCAATTAGGGTGAGTGCCGATGAGGCAATCTTCTATAGGTGTGCATTTGATGGCTACCAAGACACACTTTACACACATGTCCAACGCCAATTCTACCGCGACTGCACAATCTCCGGTACCATTGACTTTGTTTTCGGTGATGCTGCCGCCATTTTCCAAAACTGCACTTTTGTGGTGCGCAAGCCAATGCCGAACCAGTCTTGCATTGTCACAGCTCAAGGCCGGAAAGAGAGGCGCCAGCCAACTGGAATCATCATTATAAACAGCAGATTTACCTCTGATGCCGCATACTACCCTCTGAGGCATGAAAACAAGGCATATCTTGGCCGTCCATGGAAGGAGTACTCGAGGACCATCATCATGGAGTCATACATTGATGACTTCATTCGGCCACAAGGTTGGTTACCGTGGGAGGCCGATTTCGGGCTCAAGACATGCTTCTATGCTGAGTACAACAATCGTGGCCCTGGTGCTAGCAAGGCGAAGCGTGTGACATGGCACTGCATCAAGAACATTACGCCGCAGCATGCTTTGGATTTCACACCCGGCAGGTTCTTTAAGGGTGATCGATGGGTTAGGCTATCTGGAGTGCCTTATTTTCCTGGCCTCACTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

265

Amino Acids

30.08

Weight (kDa)

9.19

Isoelectric Point (pI)

30.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pectinesterase	PF01095	1 - 249	1.4e-118	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	440
Acc16I	TGCGCA	1 cut(s)	359
Acc36I	ACCTGC	1 cut(s)	720
Acc65I	GGTACC	1 cut(s)	296
AccB1I	GGYRCC	3 cut(s)	74, 296, 414
AccI	GTMKAC	1 cut(s)	69
AccII	CGCG	1 cut(s)	279
AciI	CCGC	6 cut(s)	93, 104, 277, 327, 465, 701
AcoI	YGGCCR	2 cut(s)	505, 569
AcyI	GRCGYC	1 cut(s)	415
AfaI	GTAC	3 cut(s)	298, 524, 632
AfiI	CCNNNNNNNGG	1 cut(s)	603
AflIII	ACRYGT	1 cut(s)	256
AgsI	TTSAA	1 cut(s)	61
AjnI	CCWGG	2 cut(s)	646, 781
AleI	CACNNNNGTG	1 cut(s)	350
AluBI	AGCT	3 cut(s)	116, 176, 395
AluI	AGCT	3 cut(s)	116, 176, 395
Alw21I	GWGCWC	1 cut(s)	118
Alw26I	GTCTC	1 cut(s)	18
Ama87I	CYCGRG	1 cut(s)	526
AoxI	GGCC	6 cut(s)	400, 505, 569, 594, 643, 784
ApeKI	GCWGC	2 cut(s)	323, 703
ArsI	GACNNNNNNTTYG	2 cut(s)	296, 328
Asp718I	GGTACC	1 cut(s)	296
AspLEI	GCGC	2 cut(s)	360, 417
AspS9I	GGNCC	4 cut(s)	106, 158, 531, 644
AsuC2I	CCSGG	1 cut(s)	726
AsuHPI	GGTGA	4 cut(s)	40, 202, 329, 755
AsuNHI	GCTAGC	1 cut(s)	653
AvaI	CYCGRG	1 cut(s)	526
AvaII	GGWCC	3 cut(s)	106, 158, 531
BanI	GGYRCC	3 cut(s)	74, 296, 414
BanII	GRGCYC	2 cut(s)	118, 609
Bbv12I	GWGCWC	1 cut(s)	118
BbvI	GCAGC	2 cut(s)	310, 715
BccI	CCATC	3 cut(s)	224, 542, 744
BceAI	ACGGC	2 cut(s)	88, 492
BciT130I	CCWGG	2 cut(s)	648, 783
BcnI	CCSGG	1 cut(s)	726
BcoDI	GTCTC	1 cut(s)	18
BfaI	CTAG	2 cut(s)	129, 654
BfmI	CTRYAG	2 cut(s)	96, 214
BfoI	RGCGCY	1 cut(s)	418
BfuAI	ACCTGC	1 cut(s)	720
BglI	GCCNNNNNGGC	1 cut(s)	203
BisI	GCNGC	5 cut(s)	324, 327, 465, 701, 704
BlsI	GCNGC	5 cut(s)	325, 328, 466, 702, 705
BmcAI	AGTACT	1 cut(s)	524
Bme1390I	CCNGG	3 cut(s)	648, 726, 783
Bme18I	GGWCC	3 cut(s)	106, 158, 531
BmeT110I	CYCGRG	1 cut(s)	526
BmgT120I	GGNCC	4 cut(s)	106, 158, 531, 644
BmiI	GGNNCC	3 cut(s)	76, 298, 416
BmrFI	CCNGG	3 cut(s)	648, 726, 783
BmsI	GCATC	4 cut(s)	3, 310, 451, 693
BmtI	GCTAGC	1 cut(s)	657
BpmI	CTGGAG	1 cut(s)	786
BpuEI	CTTGAG	2 cut(s)	381, 593
BpuMI	CCSGG	1 cut(s)	726
Bsa29I	ATCGAT	1 cut(s)	748
BsaBI	GATNNNNATC	1 cut(s)	17
BsaHI	GRCGYC	1 cut(s)	415
BsaJI	CCNNGG	4 cut(s)	109, 512, 587, 646
BsaWI	WCCGGW	2 cut(s)	50, 293
Bsc4I	CCNNNNNNNGG	1 cut(s)	603
Bse118I	RCCGGY	1 cut(s)	155
Bse1I	ACTGG	2 cut(s)	376, 430
Bse8I	GATNNNNATC	1 cut(s)	17
BseBI	CCWGG	2 cut(s)	648, 783
BseCI	ATCGAT	1 cut(s)	748
BseDI	CCNNGG	4 cut(s)	109, 512, 587, 646
BseJI	GATNNNNATC	1 cut(s)	17
BseLI	CCNNNNNNNGG	1 cut(s)	603
BseMII	CTCAG	3 cut(s)	165, 470, 618
BseNI	ACTGG	2 cut(s)	376, 430
BseXI	GCAGC	2 cut(s)	310, 715
BsgI	GTGCAG	2 cut(s)	268, 328
Bsh1236I	CGCG	1 cut(s)	279
BshFI	GGCC	6 cut(s)	402, 507, 571, 596, 645, 786
BshNI	GGYRCC	3 cut(s)	74, 296, 414
BshVI	ATCGAT	1 cut(s)	748
BsiHKAI	GWGCWC	1 cut(s)	118
BsiHKCI	CYCGRG	1 cut(s)	526
BsiSI	CCGG	5 cut(s)	51, 156, 294, 403, 726
BslI	CCNNNNNNNGG	1 cut(s)	603
BsmAI	GTCTC	1 cut(s)	18
BsmBI	CGTCTC	1 cut(s)	18
BsnI	GGCC	6 cut(s)	402, 507, 571, 596, 645, 786
BsoBI	CYCGRG	1 cut(s)	526
Bsp1286I	GDGCHC	2 cut(s)	118, 609
Bsp143I	GATC	2 cut(s)	18, 745
Bsp19I	CCATGG	2 cut(s)	109, 512
BspACI	CCGC	6 cut(s)	93, 104, 277, 327, 465, 701
BspANI	GGCC	6 cut(s)	402, 507, 571, 596, 645, 786
BspCNI	CTCAG	3 cut(s)	164, 471, 619
BspDI	ATCGAT	1 cut(s)	748
BspFNI	CGCG	1 cut(s)	279
BspLI	GGNNCC	3 cut(s)	76, 298, 416
BspMI	ACCTGC	1 cut(s)	720
BspOI	GCTAGC	1 cut(s)	657
BspT107I	GGYRCC	3 cut(s)	74, 296, 414
BsrFI	RCCGGY	1 cut(s)	155
BsrI	ACTGG	2 cut(s)	376, 430
BssAI	RCCGGY	1 cut(s)	155
BssECI	CCNNGG	4 cut(s)	109, 512, 587, 646
BssMI	GATC	2 cut(s)	18, 745
BssNI	GRCGYC	1 cut(s)	415
BssT1I	CCWWGG	2 cut(s)	109, 512
Bst2UI	CCWGG	2 cut(s)	648, 783
Bst4CI	ACNGT	3 cut(s)	29, 100, 588
BstACI	GRCGYC	1 cut(s)	415
BstC8I	GCNNGC	4 cut(s)	362, 419, 655, 708
BstDEI	CTNAG	3 cut(s)	151, 479, 627
BstDSI	CCRYGG	3 cut(s)	109, 512, 587
BstEII	GGTNACC	1 cut(s)	582
BstFNI	CGCG	1 cut(s)	279
BstH2I	RGCGCY	1 cut(s)	418
BstHHI	GCGC	2 cut(s)	360, 417
BstKTI	GATC	2 cut(s)	21, 748
BstMAI	GTCTC	1 cut(s)	18
BstMBI	GATC	2 cut(s)	18, 745
BstMWI	GCNNNNNNNGC	3 cut(s)	101, 203, 659
BstNI	CCWGG	2 cut(s)	648, 783
BstNSI	RCATGY	3 cut(s)	260, 618, 710
BstPI	GGTNACC	1 cut(s)	582
BstSCI	CCNGG	3 cut(s)	646, 724, 781
BstSFI	CTRYAG	2 cut(s)	96, 214
BstUI	CGCG	1 cut(s)	279
BstV1I	GCAGC	2 cut(s)	310, 715
BstXI	CCANNNNNNTGG	3 cut(s)	425, 541, 579
Bsu15I	ATCGAT	1 cut(s)	748
BsuRI	GGCC	6 cut(s)	402, 507, 571, 596, 645, 786
BsuTUI	ATCGAT	1 cut(s)	748
BtgI	CCRYGG	3 cut(s)	109, 512, 587
BtsI	GCAGTG	2 cut(s)	679, 789
BtsIMutI	CAGTG	2 cut(s)	679, 789
BveI	ACCTGC	1 cut(s)	720
Cac8I	GCNNGC	4 cut(s)	362, 419, 655, 708
CfoI	GCGC	2 cut(s)	360, 417
Cfr10I	RCCGGY	1 cut(s)	155
Cfr13I	GGNCC	4 cut(s)	106, 158, 531, 644
ClaI	ATCGAT	1 cut(s)	748
Csp6I	GTAC	3 cut(s)	297, 523, 631
CspCI	CAANNNNNGTGG	2 cut(s)	622, 657
CviAII	CATG	9 cut(s)	110, 124, 257, 485, 513, 541, 615, 675, 707
CviQI	GTAC	3 cut(s)	297, 523, 631
DdeI	CTNAG	3 cut(s)	151, 479, 627
DinI	GGCGCC	1 cut(s)	416
DpnI	GATC	2 cut(s)	20, 747
DpnII	GATC	2 cut(s)	18, 745
EaeI	YGGCCR	2 cut(s)	505, 569
Ecl136II	GAGCTC	1 cut(s)	116
Eco130I	CCWWGG	2 cut(s)	109, 512
Eco24I	GRGCYC	2 cut(s)	118, 609
Eco47I	GGWCC	3 cut(s)	106, 158, 531
Eco53kI	GAGCTC	1 cut(s)	116
Eco88I	CYCGRG	1 cut(s)	526
Eco91I	GGTNACC	1 cut(s)	582
EcoICRI	GAGCTC	1 cut(s)	116
EcoO65I	GGTNACC	1 cut(s)	582
EcoRII	CCWGG	2 cut(s)	646, 781
EcoT14I	CCWWGG	2 cut(s)	109, 512
EcoT38I	GRGCYC	2 cut(s)	118, 609
EgeI	GGCGCC	1 cut(s)	416
EheI	GGCGCC	1 cut(s)	416
ErhI	CCWWGG	2 cut(s)	109, 512
Esp3I	CGTCTC	1 cut(s)	18
FaeI	CATG	9 cut(s)	113, 127, 260, 488, 516, 544, 618, 678, 710
FalI	AAGNNNNNCTT	4 cut(s)	231, 263, 602, 634
FatI	CATG	9 cut(s)	109, 123, 256, 484, 512, 540, 614, 674, 706
FblI	GTMKAC	1 cut(s)	69
Fnu4HI	GCNGC	5 cut(s)	324, 327, 465, 701, 704
FriOI	GRGCYC	2 cut(s)	118, 609
Fsp4HI	GCNGC	5 cut(s)	324, 327, 465, 701, 704
FspBI	CTAG	2 cut(s)	129, 654
FspI	TGCGCA	1 cut(s)	359
GlaI	GCGC	2 cut(s)	359, 416
GluI	GCNGC	5 cut(s)	324, 327, 465, 701, 704
GsuI	CTGGAG	1 cut(s)	786
HaeII	RGCGCY	1 cut(s)	418
HaeIII	GGCC	6 cut(s)	402, 507, 571, 596, 645, 786
HapII	CCGG	5 cut(s)	51, 156, 294, 403, 726
HhaI	GCGC	2 cut(s)	360, 417
Hin1I	GRCGYC	1 cut(s)	415
Hin1II	CATG	9 cut(s)	113, 127, 260, 488, 516, 544, 618, 678, 710
Hin6I	GCGC	2 cut(s)	358, 415
HinP1I	GCGC	2 cut(s)	358, 415
HinfI	GANTC	3 cut(s)	45, 429, 545
HpaII	CCGG	5 cut(s)	51, 156, 294, 403, 726
HphI	GGTGA	4 cut(s)	40, 202, 329, 755
Hpy166II	GTNNAC	1 cut(s)	70
Hpy188I	TCNGA	4 cut(s)	12, 23, 460, 480
Hpy188III	TCNNGA	4 cut(s)	161, 610, 688, 765
Hpy8I	GTNNAC	1 cut(s)	70
HpyAV	CCTTC	1 cut(s)	511
HpyCH4III	ACNGT	3 cut(s)	29, 100, 588
HpyCH4V	TGCA	5 cut(s)	224, 285, 345, 384, 684
HpyF10VI	GCNNNNNNNGC	3 cut(s)	101, 203, 659
HpyF3I	CTNAG	3 cut(s)	151, 479, 627
Hsp92I	GRCGYC	1 cut(s)	415
Hsp92II	CATG	9 cut(s)	113, 127, 260, 488, 516, 544, 618, 678, 710
HspAI	GCGC	2 cut(s)	358, 415
KasI	GGCGCC	1 cut(s)	414
KpnI	GGTACC	1 cut(s)	300
Kzo9I	GATC	2 cut(s)	18, 745
LmnI	GCTCC	1 cut(s)	113
Lsp1109I	GCAGC	2 cut(s)	310, 715
LweI	GCATC	4 cut(s)	3, 310, 451, 693
MaeI	CTAG	2 cut(s)	129, 654
MaeIII	GTNAC	3 cut(s)	388, 582, 670
MalI	GATC	2 cut(s)	20, 747
MboI	GATC	2 cut(s)	18, 745
MboII	GAAGA	1 cut(s)	202
MhlI	GDGCHC	2 cut(s)	118, 609
MluCI	AATT	2 cut(s)	183, 269
Mly113I	GGCGCC	1 cut(s)	415
MlyI	GAGTC	1 cut(s)	554
MmeI	TCCRAC	1 cut(s)	286
MnlI	CCTC	8 cut(s)	196, 405, 466, 474, 486, 522, 586, 797
MseI	TTAA	1 cut(s)	738
MslI	CAYNNNNRTG	2 cut(s)	350, 539
MspI	CCGG	5 cut(s)	51, 156, 294, 403, 726
MspR9I	CCNGG	3 cut(s)	648, 726, 783
MvaI	CCWGG	2 cut(s)	648, 783
MvnI	CGCG	1 cut(s)	279
MwoI	GCNNNNNNNGC	3 cut(s)	101, 203, 659
NarI	GGCGCC	1 cut(s)	415
NciI	CCSGG	1 cut(s)	726
NcoI	CCATGG	2 cut(s)	109, 512
NdeII	GATC	2 cut(s)	18, 745
NheI	GCTAGC	1 cut(s)	653
NlaIII	CATG	9 cut(s)	113, 127, 260, 488, 516, 544, 618, 678, 710
NlaIV	GGNNCC	3 cut(s)	76, 298, 416
NmuCI	GTSAC	2 cut(s)	388, 670
NsbI	TGCGCA	1 cut(s)	359
NspI	RCATGY	3 cut(s)	260, 618, 710
OliI	CACNNNNGTG	1 cut(s)	350
PaeI	GCATGC	1 cut(s)	710
PaeR7I	CTCGAG	1 cut(s)	526
PciI	ACATGT	1 cut(s)	256
PfeI	GAWTC	2 cut(s)	45, 429
PkrI	GCNGC	5 cut(s)	325, 328, 466, 702, 705
PleI	GAGTC	1 cut(s)	553
PluTI	GGCGCC	1 cut(s)	418
PpsI	GAGTC	1 cut(s)	553
PscI	ACATGT	1 cut(s)	256
PsiI	TTATAA	1 cut(s)	440
Psp124BI	GAGCTC	1 cut(s)	118
Psp6I	CCWGG	2 cut(s)	646, 781
PspEI	GGTNACC	1 cut(s)	582
PspGI	CCWGG	2 cut(s)	646, 781
PspN4I	GGNNCC	3 cut(s)	76, 298, 416
PspPI	GGNCC	4 cut(s)	106, 158, 531, 644
PspXI	VCTCGAGB	1 cut(s)	526
RsaI	GTAC	3 cut(s)	298, 524, 632
RsaNI	GTAC	3 cut(s)	297, 523, 631
RseI	CAYNNNNRTG	2 cut(s)	350, 539
SacI	GAGCTC	1 cut(s)	118
SaqAI	TTAA	1 cut(s)	738
SatI	GCNGC	5 cut(s)	324, 327, 465, 701, 704
Sau3AI	GATC	2 cut(s)	18, 745
Sau96I	GGNCC	4 cut(s)	106, 158, 531, 644
ScaI	AGTACT	1 cut(s)	524
SchI	GAGTC	1 cut(s)	554
ScrFI	CCNGG	3 cut(s)	648, 726, 783
SduI	GDGCHC	2 cut(s)	118, 609
SetI	ASST	8 cut(s)	86, 118, 178, 221, 397, 458, 580, 734
SfaNI	GCATC	4 cut(s)	3, 310, 451, 693
SfcI	CTRYAG	2 cut(s)	96, 214
SfoI	GGCGCC	1 cut(s)	416
Sfr274I	CTCGAG	1 cut(s)	526
SinI	GGWCC	3 cut(s)	106, 158, 531
SlaI	CTCGAG	1 cut(s)	526
SmiMI	CAYNNNNRTG	2 cut(s)	350, 539
SmlI	CTYRAG	3 cut(s)	396, 526, 608
SmoI	CTYRAG	3 cut(s)	396, 526, 608
SphI	GCATGC	1 cut(s)	710
Sse9I	AATT	2 cut(s)	183, 269
SsiI	CCGC	6 cut(s)	93, 104, 277, 327, 465, 701
SspDI	GGCGCC	1 cut(s)	414
SspMI	CTAG	2 cut(s)	129, 654
SstI	GAGCTC	1 cut(s)	118
StyD4I	CCNGG	3 cut(s)	646, 724, 781
StyI	CCWWGG	2 cut(s)	109, 512
TaaI	ACNGT	3 cut(s)	29, 100, 588
TaqI	TCGA	2 cut(s)	527, 748
TasI	AATT	2 cut(s)	183, 269
TatI	WGTACW	2 cut(s)	522, 630
TauI	GCSGC	3 cut(s)	329, 467, 703
TfiI	GAWTC	2 cut(s)	45, 429
Tru1I	TTAA	1 cut(s)	738
Tru9I	TTAA	1 cut(s)	738
TscAI	CASTG	2 cut(s)	686, 796
TseFI	GTSAC	2 cut(s)	388, 670
TseI	GCWGC	2 cut(s)	323, 703
Tsp45I	GTSAC	2 cut(s)	388, 670
TspDTI	ATGAA	3 cut(s)	112, 501, 553
TspRI	CASTG	2 cut(s)	686, 796
VpaK11BI	GGWCC	3 cut(s)	106, 158, 531
XceI	RCATGY	3 cut(s)	260, 618, 710
XhoI	CTCGAG	1 cut(s)	526
XmiI	GTMKAC	1 cut(s)	69
XspI	CTAG	2 cut(s)	129, 654
ZrmI	AGTACT	1 cut(s)	524

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.