Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Forward (+)

12991852 .. 12993423

Gene Structure 1572 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1164600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGGGCCAAGCAGATGGATCTAACAAGAAGAAAAAGCTCGCCATCATCGGCGCCTCTGCCCTGATTCTGGTGGCAATGGTAGTTGCTGTAACTGTTGGGGTCACAGTATCACGCCGCGGTGGAGGGTCATCCGACGGCCACACCTCCACGTCCACAAAGGCGATCCAGGCCATTTGCCAGCCCACCGACTATAAGAAGACATGCGAGGAAAGCCTGTCAGGCGCGGCCGGTAATGTCACCGATCCCAAAGAGTTGATCAAAGTAGGGTTCCAAGTCACCATGGACATGCTCCGCGGCGTCATTGAGAAGTCCACCACGTTGAAGGATTTGGCTAAGGACCCCAGCACCGGAGATGCACTCCAAAACTGCAAGGAGCTCCTGGAGTACGCCATCGATGATTTAGGTGACTCGTTTGACAGGATTGGGGCCTTCGACATGAGCAAGCTCGATCTTTACGTGGAGGATCTCAAAGTGTGGCTCAGCGCTGCCATGACATACGAGCAGACTTGCTTGGATGGGTTCGAGAATACCACTGGTGACGCCGGTGAGAAGATGAGGGAGTTCTTGAAGACATCGCAGGAGCTCACCAACAACGGCCTCGCCATGGTCGACCAAGTTTCCACGATGTTCGGGGCTCTTAATGTCAAATCCGGCCGCAGACTCCTTGAGGCTACAGCTGGAACTGGTGCGAAGAAGTTCCAGAAGGCTAAGGTCATCCCTGCTTGGATTGATAATCGAAGGCTTGACCTCGCTACCGCCACTCCTCAGACGCTGAAACCCGATGTGGTGGTGGCCAAAAAAGGGGGTGGTAAGTACACGACTGTCAACGAAGCCTTGAAGGATATCCCCAAGGACAACGCAGTGAAGGTCTTTGTGATTTATGTTAAGGAGGGAGTGTATGAGGAGCACGTCCTGTTTGATAAGCACATGACCAATGTCATGCTTATAGGAGATGGCCCCACTAAGACCATTATTACCGGTAGAAAGAACTTTGCAGAGGGTACCAAGACAATGCAGACTGCCACAGTCGGTGTTGTGGGAGACTACTTCATTGCCAAGGACATTGGATTTGAGAACACAGCCGGAGCTGTCGGACACCAAGCCGTGGCTCTACGTGTACAGTCAGACTTGTCCATCTTCTACAACTGCAAGATGGATGGGTACCAAGATACCCTATACACCCAAACTCACAGGCAATTCTACCGCGACTGCACCATCAGTGGAACCATCGACTTCATCTTTGGTGATGCTGCCGCTGTGTTCCAAAACTGCAAGATGATCGTCAGGAAGCCACTCGAGAACCAGGCCTGCATGGTGACTGCCCACGGTAGACTCGACAGACGTTCTCCCTCGGGTATCATCCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

456

Amino Acids

49.36

Weight (kDa)

7.52

Isoelectric Point (pI)

23.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	53 - 202	3.5e-33	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	261 - 455	6.9e-88	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	1001, 1161
AccB1I	GGYRCC	3 cut(s)	50, 1001, 1161
AccB7I	CCANNNNNTGG	1 cut(s)	1105
AccI	GTMKAC	2 cut(s)	609, 1330
AccII	CGCG	4 cut(s)	117, 224, 294, 1206
AciI	CCGC	9 cut(s)	115, 117, 224, 292, 294, 655, 756, 1204, 1254
AclWI	GGATC	4 cut(s)	25, 157, 236, 471
AcoI	YGGCCR	4 cut(s)	136, 225, 652, 792
AcyI	GRCGYC	3 cut(s)	51, 297, 540
AfaI	GTAC	5 cut(s)	386, 815, 1003, 1119, 1163
AfeI	AGCGCT	1 cut(s)	484
AfiI	CCNNNNNNNGG	5 cut(s)	67, 347, 604, 801, 1105
AflIII	ACRYGT	1 cut(s)	1114
AgeI	ACCGGT	1 cut(s)	977
AgsI	TTSAA	3 cut(s)	322, 568, 838
AjiI	CACGTC	2 cut(s)	150, 910
AjnI	CCWGG	3 cut(s)	165, 378, 1302
AluBI	AGCT	6 cut(s)	37, 376, 445, 583, 677, 1088
AluI	AGCT	6 cut(s)	37, 376, 445, 583, 677, 1088
Alw21I	GWGCWC	3 cut(s)	378, 585, 909
Alw26I	GTCTC	1 cut(s)	1035
AlwI	GGATC	4 cut(s)	25, 157, 236, 471
AlwNI	CAGNNNCTG	1 cut(s)	772
Ama87I	CYCGRG	2 cut(s)	1295, 1351
Aor51HI	AGCGCT	1 cut(s)	484
ApeKI	GCWGC	2 cut(s)	485, 1250
ArsI	GACNNNNNNTTYG	4 cut(s)	1052, 1084, 1223, 1255
AsiGI	ACCGGT	1 cut(s)	977
Asp700I	GAANNNNTTC	1 cut(s)	695
Asp718I	GGTACC	2 cut(s)	1001, 1161
AspLEI	GCGC	3 cut(s)	53, 224, 485
AspS9I	GGNCC	4 cut(s)	4, 337, 426, 956
AsuHPI	GGTGA	8 cut(s)	229, 268, 416, 548, 557, 577, 1256, 1327
AvaI	CYCGRG	2 cut(s)	1295, 1351
AvaII	GGWCC	1 cut(s)	337
BalI	TGGCCA	1 cut(s)	794
BanI	GGYRCC	3 cut(s)	50, 1001, 1161
BanII	GRGCYC	3 cut(s)	378, 585, 637
BbsI	GAAGAC	2 cut(s)	203, 575
Bbv12I	GWGCWC	3 cut(s)	378, 585, 909
BbvI	GCAGC	2 cut(s)	472, 1237
BceAI	ACGGC	3 cut(s)	151, 610, 1088
BcgI	CGANNNNNNTGC	2 cut(s)	1261, 1295
BciT130I	CCWGG	3 cut(s)	167, 380, 1304
BclI	TGATCA	1 cut(s)	255
BcoDI	GTCTC	1 cut(s)	1035
BfmI	CTRYAG	1 cut(s)	672
BfoI	RGCGCY	2 cut(s)	54, 486
BglI	GCCNNNNNGGC	1 cut(s)	219
BisI	GCNGC	7 cut(s)	115, 225, 295, 486, 655, 1251, 1254
BlpI	GCTNAGC	1 cut(s)	479
BlsI	GCNGC	7 cut(s)	116, 226, 296, 487, 656, 1252, 1255
Bme1390I	CCNGG	3 cut(s)	167, 380, 1304
Bme18I	GGWCC	1 cut(s)	337
BmeT110I	CYCGRG	2 cut(s)	1295, 1351
BmgBI	CACGTC	2 cut(s)	150, 910
BmgT120I	GGNCC	4 cut(s)	4, 337, 426, 956
BmiI	GGNNCC	8 cut(s)	52, 269, 339, 427, 958, 1003, 1163, 1225
BmrFI	CCNGG	3 cut(s)	167, 380, 1304
BmsI	GCATC	2 cut(s)	343, 1237
BoxI	GACNNNNGTC	1 cut(s)	935
BpiI	GAAGAC	2 cut(s)	203, 575
BplI	GAGNNNNNCTC	2 cut(s)	342, 374
BpmI	CTGGAG	1 cut(s)	401
Bpu10I	CCTNAGC	2 cut(s)	333, 708
Bpu1102I	GCTNAGC	1 cut(s)	479
BpuEI	CTTGAG	1 cut(s)	686
Bsa29I	ATCGAT	1 cut(s)	393
BsaAI	YACGTR	2 cut(s)	457, 1115
BsaHI	GRCGYC	3 cut(s)	51, 297, 540
BsaJI	CCNNGG	9 cut(s)	115, 279, 292, 603, 849, 1056, 1104, 1324, 1350
BsaWI	WCCGGW	2 cut(s)	347, 977
BsaXI	ACNNNNNCTCC	4 cut(s)	745, 775, 881, 911
Bsc4I	CCNNNNNNNGG	5 cut(s)	67, 347, 604, 801, 1105
Bse118I	RCCGGY	3 cut(s)	227, 542, 977
Bse1I	ACTGG	2 cut(s)	538, 688
Bse3DI	GCAATG	2 cut(s)	81, 1050
BseBI	CCWGG	3 cut(s)	167, 380, 1304
BseCI	ATCGAT	1 cut(s)	393
BseDI	CCNNGG	9 cut(s)	115, 279, 292, 603, 849, 1056, 1104, 1324, 1350
BseGI	GGATG	5 cut(s)	128, 520, 714, 1162, 1359
BseLI	CCNNNNNNNGG	5 cut(s)	67, 347, 604, 801, 1105
BseMI	GCAATG	2 cut(s)	81, 1050
BseMII	CTCAG	2 cut(s)	493, 779
BseNI	ACTGG	2 cut(s)	538, 688
BseRI	GAGGAG	2 cut(s)	753, 917
BseX3I	CGGCCG	2 cut(s)	225, 652
BseXI	GCAGC	2 cut(s)	472, 1237
BseYI	CCCAGC	1 cut(s)	341
BsgI	GTGCAG	1 cut(s)	1195
Bsh1236I	CGCG	4 cut(s)	117, 224, 294, 1206
Bsh1285I	CGRYCG	2 cut(s)	228, 655
BshNI	GGYRCC	3 cut(s)	50, 1001, 1161
BshTI	ACCGGT	1 cut(s)	977
BshVI	ATCGAT	1 cut(s)	393
BsiEI	CGRYCG	2 cut(s)	228, 655
BsiHKAI	GWGCWC	3 cut(s)	378, 585, 909
BsiHKCI	CYCGRG	2 cut(s)	1295, 1351
BsiSI	CCGG	6 cut(s)	228, 348, 543, 651, 978, 1083
BslI	CCNNNNNNNGG	5 cut(s)	67, 347, 604, 801, 1105
BsmAI	GTCTC	1 cut(s)	1035
BsoBI	CYCGRG	2 cut(s)	1295, 1351
Bsp1286I	GDGCHC	4 cut(s)	378, 585, 637, 909
Bsp1407I	TGTACA	1 cut(s)	1117
Bsp143I	GATC	7 cut(s)	17, 162, 241, 255, 448, 463, 1278
Bsp1720I	GCTNAGC	1 cut(s)	479
Bsp19I	CCATGG	2 cut(s)	279, 603
BspACI	CCGC	9 cut(s)	115, 117, 224, 292, 294, 655, 756, 1204, 1254
BspCNI	CTCAG	2 cut(s)	492, 778
BspDI	ATCGAT	1 cut(s)	393
BspFNI	CGCG	4 cut(s)	117, 224, 294, 1206
BspLI	GGNNCC	8 cut(s)	52, 269, 339, 427, 958, 1003, 1163, 1225
BspPI	GGATC	4 cut(s)	25, 157, 236, 471
BspT107I	GGYRCC	3 cut(s)	50, 1001, 1161
BsrDI	GCAATG	2 cut(s)	81, 1050
BsrFI	RCCGGY	3 cut(s)	227, 542, 977
BsrGI	TGTACA	1 cut(s)	1117
BsrI	ACTGG	2 cut(s)	538, 688
BssAI	RCCGGY	3 cut(s)	227, 542, 977
BssECI	CCNNGG	9 cut(s)	115, 279, 292, 603, 849, 1056, 1104, 1324, 1350
BssMI	GATC	7 cut(s)	17, 162, 241, 255, 448, 463, 1278
BssNI	GRCGYC	3 cut(s)	51, 297, 540
BssT1I	CCWWGG	4 cut(s)	279, 603, 849, 1056
Bst2UI	CCWGG	3 cut(s)	167, 380, 1304
Bst4CI	ACNGT	6 cut(s)	94, 106, 823, 1027, 1122, 1328
BstACI	GRCGYC	3 cut(s)	51, 297, 540
BstAUI	TGTACA	1 cut(s)	1117
BstBAI	YACGTR	2 cut(s)	457, 1115
BstC8I	GCNNGC	4 cut(s)	39, 179, 443, 1309
BstDEI	CTNAG	5 cut(s)	333, 479, 708, 765, 963
BstDSI	CCRYGG	6 cut(s)	115, 279, 292, 603, 1104, 1324
BstF5I	GGATG	5 cut(s)	128, 520, 714, 1162, 1359
BstFNI	CGCG	4 cut(s)	117, 224, 294, 1206
BstH2I	RGCGCY	2 cut(s)	54, 486
BstHHI	GCGC	3 cut(s)	53, 224, 485
BstKTI	GATC	7 cut(s)	20, 165, 244, 258, 451, 466, 1281
BstMAI	GTCTC	1 cut(s)	1035
BstMBI	GATC	7 cut(s)	17, 162, 241, 255, 448, 463, 1278
BstMCI	CGRYCG	2 cut(s)	228, 655
BstMWI	GCNNNNNNNGC	3 cut(s)	167, 210, 219
BstNI	CCWGG	3 cut(s)	167, 380, 1304
BstNSI	RCATGY	2 cut(s)	204, 289
BstPAI	GACNNNNGTC	1 cut(s)	935
BstSCI	CCNGG	3 cut(s)	165, 378, 1302
BstSFI	CTRYAG	1 cut(s)	672
BstUI	CGCG	4 cut(s)	117, 224, 294, 1206
BstV1I	GCAGC	2 cut(s)	472, 1237
BstV2I	GAAGAC	2 cut(s)	203, 575
BstX2I	RGATCY	2 cut(s)	17, 463
BstXI	CCANNNNNNTGG	1 cut(s)	14
BstYI	RGATCY	2 cut(s)	17, 463
BstZI	CGGCCG	2 cut(s)	225, 652
Bsu15I	ATCGAT	1 cut(s)	393
BsuTUI	ATCGAT	1 cut(s)	393
BtgI	CCRYGG	6 cut(s)	115, 279, 292, 603, 1104, 1324
BtgZI	GCGATG	1 cut(s)	558
BtrI	CACGTC	2 cut(s)	150, 910
BtsCI	GGATG	5 cut(s)	128, 520, 714, 1162, 1359
BtsI	GCAGTG	1 cut(s)	867
BtsIMutI	CAGTG	3 cut(s)	531, 867, 1225
Cac8I	GCNNGC	4 cut(s)	39, 179, 443, 1309
CaiI	CAGNNNCTG	1 cut(s)	772
CfoI	GCGC	3 cut(s)	53, 224, 485
Cfr10I	RCCGGY	3 cut(s)	227, 542, 977
Cfr13I	GGNCC	4 cut(s)	4, 337, 426, 956
Cfr42I	CCGCGG	2 cut(s)	118, 295
ClaI	ATCGAT	1 cut(s)	393
CseI	GACGC	3 cut(s)	286, 548, 778
Csp6I	GTAC	5 cut(s)	385, 814, 1002, 1118, 1162
CspAI	ACCGGT	1 cut(s)	977
CviAII	CATG	9 cut(s)	201, 280, 286, 436, 490, 604, 928, 940, 1312
CviQI	GTAC	5 cut(s)	385, 814, 1002, 1118, 1162
DdeI	CTNAG	5 cut(s)	333, 479, 708, 765, 963
DinI	GGCGCC	1 cut(s)	52
DpnI	GATC	7 cut(s)	19, 164, 243, 257, 450, 465, 1280
DpnII	GATC	7 cut(s)	17, 162, 241, 255, 448, 463, 1278
EaeI	YGGCCR	4 cut(s)	136, 225, 652, 792
EagI	CGGCCG	2 cut(s)	225, 652
Ecl136II	GAGCTC	2 cut(s)	376, 583
EclXI	CGGCCG	2 cut(s)	225, 652
Eco130I	CCWWGG	4 cut(s)	279, 603, 849, 1056
Eco147I	AGGCCT	1 cut(s)	1307
Eco24I	GRGCYC	3 cut(s)	378, 585, 637
Eco32I	GATATC	1 cut(s)	844
Eco47I	GGWCC	1 cut(s)	337
Eco47III	AGCGCT	1 cut(s)	484
Eco52I	CGGCCG	2 cut(s)	225, 652
Eco53kI	GAGCTC	2 cut(s)	376, 583
Eco88I	CYCGRG	2 cut(s)	1295, 1351
EcoICRI	GAGCTC	2 cut(s)	376, 583
EcoO109I	RGGNCCY	2 cut(s)	337, 426
EcoRII	CCWGG	3 cut(s)	165, 378, 1302
EcoRV	GATATC	1 cut(s)	844
EcoT14I	CCWWGG	4 cut(s)	279, 603, 849, 1056
EcoT38I	GRGCYC	3 cut(s)	378, 585, 637
EgeI	GGCGCC	1 cut(s)	52
EheI	GGCGCC	1 cut(s)	52
ErhI	CCWWGG	4 cut(s)	279, 603, 849, 1056
FaeI	CATG	9 cut(s)	204, 283, 289, 439, 493, 607, 931, 943, 1315
FatI	CATG	9 cut(s)	200, 279, 285, 435, 489, 603, 927, 939, 1311
FbaI	TGATCA	1 cut(s)	255
FblI	GTMKAC	2 cut(s)	609, 1330
Fnu4HI	GCNGC	7 cut(s)	115, 225, 295, 486, 655, 1251, 1254
FokI	GGATG	5 cut(s)	115, 527, 701, 1169, 1346
FriOI	GRGCYC	3 cut(s)	378, 585, 637
Fsp4HI	GCNGC	7 cut(s)	115, 225, 295, 486, 655, 1251, 1254
GlaI	GCGC	3 cut(s)	52, 223, 484
GluI	GCNGC	7 cut(s)	115, 225, 295, 486, 655, 1251, 1254
GsaI	CCCAGC	1 cut(s)	345
GsuI	CTGGAG	1 cut(s)	401
HaeII	RGCGCY	2 cut(s)	54, 486
HapII	CCGG	6 cut(s)	228, 348, 543, 651, 978, 1083
HgaI	GACGC	3 cut(s)	286, 548, 778
HhaI	GCGC	3 cut(s)	53, 224, 485
Hin1I	GRCGYC	3 cut(s)	51, 297, 540
Hin1II	CATG	9 cut(s)	204, 283, 289, 439, 493, 607, 931, 943, 1315
Hin6I	GCGC	3 cut(s)	51, 222, 483
HinP1I	GCGC	3 cut(s)	51, 222, 483
HincII	GTYRAC	2 cut(s)	610, 826
HindII	GTYRAC	2 cut(s)	610, 826
HinfI	GANTC	4 cut(s)	64, 407, 660, 1332
HpaII	CCGG	6 cut(s)	228, 348, 543, 651, 978, 1083
HphI	GGTGA	8 cut(s)	229, 268, 416, 548, 557, 577, 1256, 1327
Hpy166II	GTNNAC	7 cut(s)	153, 312, 610, 816, 826, 1118, 1331
Hpy188I	TCNGA	4 cut(s)	133, 768, 1094, 1126
Hpy188III	TCNNGA	5 cut(s)	523, 565, 700, 1285, 1297
Hpy8I	GTNNAC	7 cut(s)	153, 312, 610, 816, 826, 1118, 1331
Hpy99I	CGWCG	1 cut(s)	137
HpyAV	CCTTC	6 cut(s)	316, 439, 697, 732, 832, 859
HpyCH4III	ACNGT	6 cut(s)	94, 106, 823, 1027, 1122, 1328
HpyCH4IV	ACGT	6 cut(s)	149, 317, 456, 909, 1114, 1342
HpyCH4V	TGCA	8 cut(s)	356, 369, 995, 1015, 1149, 1212, 1272, 1311
HpyF10VI	GCNNNNNNNGC	3 cut(s)	167, 210, 219
HpyF3I	CTNAG	5 cut(s)	333, 479, 708, 765, 963
HpySE526I	ACGT	6 cut(s)	149, 317, 456, 909, 1114, 1342
Hsp92I	GRCGYC	3 cut(s)	51, 297, 540
Hsp92II	CATG	9 cut(s)	204, 283, 289, 439, 493, 607, 931, 943, 1315
HspAI	GCGC	3 cut(s)	51, 222, 483
KasI	GGCGCC	1 cut(s)	50
KpnI	GGTACC	2 cut(s)	1005, 1165
Ksp22I	TGATCA	1 cut(s)	255
KspI	CCGCGG	2 cut(s)	118, 295
Kzo9I	GATC	7 cut(s)	17, 162, 241, 255, 448, 463, 1278
LmnI	GCTCC	6 cut(s)	294, 373, 381, 580, 904, 1085
Lsp1109I	GCAGC	2 cut(s)	472, 1237
LweI	GCATC	2 cut(s)	343, 1237
MaeII	ACGT	6 cut(s)	149, 317, 456, 909, 1114, 1342
MaeIII	GTNAC	7 cut(s)	88, 100, 235, 274, 404, 536, 1315
MalI	GATC	7 cut(s)	19, 164, 243, 257, 450, 465, 1280
MboI	GATC	7 cut(s)	17, 162, 241, 255, 448, 463, 1278
MboII	GAAGA	6 cut(s)	40, 208, 562, 580, 703, 1129
MflI	RGATCY	2 cut(s)	17, 463
MhlI	GDGCHC	4 cut(s)	378, 585, 637, 909
MlsI	TGGCCA	1 cut(s)	794
MluCI	AATT	1 cut(s)	1196
MluNI	TGGCCA	1 cut(s)	794
Mly113I	GGCGCC	1 cut(s)	51
MlyI	GAGTC	3 cut(s)	401, 654, 1326
MmeI	TCCRAC	2 cut(s)	156, 1072
Mox20I	TGGCCA	1 cut(s)	794
MroXI	GAANNNNTTC	1 cut(s)	695
MscI	TGGCCA	1 cut(s)	794
MseI	TTAA	2 cut(s)	639, 885
MslI	CAYNNNNRTG	1 cut(s)	284
Msp20I	TGGCCA	1 cut(s)	794
MspA1I	CMGCKG	4 cut(s)	117, 294, 677, 1256
MspI	CCGG	6 cut(s)	228, 348, 543, 651, 978, 1083
MspR9I	CCNGG	3 cut(s)	167, 380, 1304
MvaI	CCWGG	3 cut(s)	167, 380, 1304
MvnI	CGCG	4 cut(s)	117, 224, 294, 1206
MwoI	GCNNNNNNNGC	3 cut(s)	167, 210, 219
NarI	GGCGCC	1 cut(s)	51
NcoI	CCATGG	2 cut(s)	279, 603
NdeII	GATC	7 cut(s)	17, 162, 241, 255, 448, 463, 1278
NlaIII	CATG	9 cut(s)	204, 283, 289, 439, 493, 607, 931, 943, 1315
NlaIV	GGNNCC	8 cut(s)	52, 269, 339, 427, 958, 1003, 1163, 1225
NmuCI	GTSAC	6 cut(s)	100, 235, 274, 404, 536, 1315
NspI	RCATGY	2 cut(s)	204, 289
PaeR7I	CTCGAG	1 cut(s)	1295
PceI	AGGCCT	1 cut(s)	1307
PcsI	WCGNNNNNNNCGW	3 cut(s)	453, 606, 1332
PdmI	GAANNNNTTC	1 cut(s)	695
PfeI	GAWTC	1 cut(s)	64
PflMI	CCANNNNNTGG	1 cut(s)	1105
PfoI	TCCNGGA	1 cut(s)	378
PinAI	ACCGGT	1 cut(s)	977
PkrI	GCNGC	7 cut(s)	116, 226, 296, 487, 656, 1252, 1255
PleI	GAGTC	3 cut(s)	401, 654, 1326
PluTI	GGCGCC	1 cut(s)	54
PpsI	GAGTC	3 cut(s)	401, 654, 1326
Ppu21I	YACGTR	2 cut(s)	457, 1115
PpuMI	RGGWCCY	1 cut(s)	337
PshAI	GACNNNNGTC	1 cut(s)	935
Psp124BI	GAGCTC	2 cut(s)	378, 585
Psp5II	RGGWCCY	1 cut(s)	337
Psp6I	CCWGG	3 cut(s)	165, 378, 1302
PspFI	CCCAGC	1 cut(s)	341
PspGI	CCWGG	3 cut(s)	165, 378, 1302
PspN4I	GGNNCC	8 cut(s)	52, 269, 339, 427, 958, 1003, 1163, 1225
PspPI	GGNCC	4 cut(s)	4, 337, 426, 956
PspPPI	RGGWCCY	1 cut(s)	337
PstNI	CAGNNNCTG	1 cut(s)	772
PsuI	RGATCY	2 cut(s)	17, 463
PvuII	CAGCTG	1 cut(s)	677
RsaI	GTAC	5 cut(s)	386, 815, 1003, 1119, 1163
RsaNI	GTAC	5 cut(s)	385, 814, 1002, 1118, 1162
RseI	CAYNNNNRTG	1 cut(s)	284
SacI	GAGCTC	2 cut(s)	378, 585
SacII	CCGCGG	2 cut(s)	118, 295
SalI	GTCGAC	1 cut(s)	608
SaqAI	TTAA	2 cut(s)	639, 885
SatI	GCNGC	7 cut(s)	115, 225, 295, 486, 655, 1251, 1254
Sau3AI	GATC	7 cut(s)	17, 162, 241, 255, 448, 463, 1278
Sau96I	GGNCC	4 cut(s)	4, 337, 426, 956
SchI	GAGTC	3 cut(s)	401, 654, 1326
ScrFI	CCNGG	3 cut(s)	167, 380, 1304
SduI	GDGCHC	4 cut(s)	378, 585, 637, 909
SfaNI	GCATC	2 cut(s)	343, 1237
SfcI	CTRYAG	1 cut(s)	672
SfoI	GGCGCC	1 cut(s)	52
Sfr274I	CTCGAG	1 cut(s)	1295
Sfr303I	CCGCGG	2 cut(s)	118, 295
SgrAI	CRCCGGYG	1 cut(s)	542
SgrBI	CCGCGG	2 cut(s)	118, 295
SinI	GGWCC	1 cut(s)	337
SlaI	CTCGAG	1 cut(s)	1295
SmiMI	CAYNNNNRTG	1 cut(s)	284
SmlI	CTYRAG	2 cut(s)	665, 1295
SmoI	CTYRAG	2 cut(s)	665, 1295
Sse9I	AATT	1 cut(s)	1196
SseBI	AGGCCT	1 cut(s)	1307
SsiI	CCGC	9 cut(s)	115, 117, 224, 292, 294, 655, 756, 1204, 1254
SspDI	GGCGCC	1 cut(s)	50
SstI	GAGCTC	2 cut(s)	378, 585
StuI	AGGCCT	1 cut(s)	1307
StyD4I	CCNGG	3 cut(s)	165, 378, 1302
StyI	CCWWGG	4 cut(s)	279, 603, 849, 1056
TaaI	ACNGT	6 cut(s)	94, 106, 823, 1027, 1122, 1328
TaiI	ACGT	6 cut(s)	152, 320, 459, 912, 1117, 1345
TaqI	TCGA	9 cut(s)	393, 432, 447, 522, 609, 736, 1230, 1296, 1335
TasI	AATT	1 cut(s)	1196
TatI	WGTACW	2 cut(s)	813, 1117
TauI	GCSGC	5 cut(s)	117, 227, 297, 657, 1256
TfiI	GAWTC	1 cut(s)	64
Tru1I	TTAA	2 cut(s)	639, 885
Tru9I	TTAA	2 cut(s)	639, 885
TscAI	CASTG	3 cut(s)	538, 867, 1225
TseFI	GTSAC	6 cut(s)	100, 235, 274, 404, 536, 1315
TseI	GCWGC	2 cut(s)	485, 1250
Tsp45I	GTSAC	6 cut(s)	100, 235, 274, 404, 536, 1315
TspDTI	ATGAA	2 cut(s)	1039, 1225
TspRI	CASTG	3 cut(s)	538, 867, 1225
Van91I	CCANNNNNTGG	1 cut(s)	1105
VpaK11BI	GGWCC	1 cut(s)	337
XceI	RCATGY	2 cut(s)	204, 289
XhoI	CTCGAG	1 cut(s)	1295
XmiI	GTMKAC	2 cut(s)	609, 1330
XmnI	GAANNNNTTC	1 cut(s)	695

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.