Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Reverse (-)

5860033 .. 5861956

Gene Structure 1924 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG076200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1791 bp

ATGGCGTACGAGGAATCAAGTAGCTCAAAAAAAAGGCTTGCCATTATTGGCATCTCTTCGACACTATTAATAGCACTAGTGGTGGCCGCGACTGTTAGTGTTGGCCTAAAAACCACTACCCATGACAACGGCAGCAGGACTTCTGTCAAGTCACATGAGATATCTGCCTCCATGAAAGCCATAATTTCCATTTGCCAGCCCACCAATTACAAACAAGAATGTGTGAATAGCCTCCTATCCGCATCTGGAAACACCACCGACCCAAAAAAGCTCATTCAGGTTGGATTCCAAGTTGCAATGAAACAAATCTCCGTGGCCGCGAAGAACTCCACTCTCTTGCAACAGCTTGAGAAGGACCCTAGAGCTAGCAAGGCCCTTGACACATGCAAAGAACTCTTGGGATTGGCTGTTACCGAGCTCAAGCAAGCTTTCGACAGATTGGGGAGTGACTTTGACATCACTGAAATTGGTACAATGATCATGGATTTGAAAGTCTGGATAAGCGCTACTGTCACATACCAAGAGACATGCTTGGACGCATTTGAGAATAGTACTAGTGATGTCGGTGAGAAGATGAAGATGATATTGAAGAGCTCCATGCATTTGAGCAGCAATTCCCTTGCCATGGTCTCCACAACCTCTTCATTCCTCAGTGGCTTAGACCACAAAGGCCTAAGCCGCCAGCTCCTTGCGGATGATGGCCTCCCTGTTCTTGGTCACGAGGATTTTGAACTTCCTGATGATTTTAATAATCTTAATGATCTTAATAATACATGGTATTGGCCTGGATTTAGGAAACTCCTTACTGCTGCTAGTAGAGCTGACGTATACAAAGTTAATAAGGCCGCGAACATTATAGTGGCCAAGGATGGAAGTGGAAAGTACAAGACCATCAATGAGGCCCTACATCATGTACCTAAGCATAGCAACATGACCTTTGTCATCTACATCAAGGAAGGTGTCTACAATGAGCTTGTCCACATTACCAAGGCCATGACCAATCTGATGCTGATCGGAGACGGTGCTAATAAGACCAGAATCACCGGAAACTTGAACTTCGTAGACGGCACCCCTACCTACAAAACCGCTACAGTCGCGGTCCATGGAGCTCACTTCATGGCTAAGGATATTGGGTTTGAGAACTCAGCCGGTCCAGACAAACATCAAGCTGTGGCAATTAGGGTGAGTGCCGATGAGGCAATCTTCTATAGGTGTGCATTTGATGGCTACCAAGACACACTTTACACACATGTCCAACGCCAATTCTACCGCGACTGCACAATCTCCGGTACCATTGACTTTGTTTTCGGTGATGCTGCCGCCATTTTCCAAAACTGCACTTTTGTGGTGCGCAAGCCAATGCCGAACCAGTCTTGCATTGTCACAGCTCAAGGCCGGAAAGAGAGGCGCCAGCCAACTGGAATCATCATTATAAACAGCATATTTACCTCTGATGCCGCATACTACCATCTGAGGCATGAAAACAAGGCATATCTTGGCCGTCCATGGAAGGAGTACTCGAGGACCATCATCATGGAGTCATATATTGATGACTTCATTCGGCCACAAGGTTGGTTACCCTGGGAGGCCGATTTCGGGCTCAAGACATGCTTCTATGCTGAGTACAACAATCGTGGCCCTGGTGCTAGCAAGGCGAAGCGTGTGACATGGCATTGCATCAAGAACATTACGCCGCAGCATGCATTGGATTTCACACCTGGCAGGTTCTTTAAGGGTGATCGATGGGTTAGGCTATCTGGAGTGCCTTATTTTCCTGGCCTCACTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

596

Amino Acids

66.22

Weight (kDa)

8.68

Isoelectric Point (pI)

29.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	60 - 209	3.1e-29	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	284 - 580	3.2e-139	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1433
Acc16I	TGCGCA	1 cut(s)	1352
Acc36I	ACCTGC	1 cut(s)	1713
Acc65I	GGTACC	1 cut(s)	1289
AccB1I	GGYRCC	3 cut(s)	1067, 1289, 1407
AccI	GTMKAC	3 cut(s)	828, 963, 1062
AccII	CGCG	5 cut(s)	89, 320, 848, 1097, 1272
AcoI	YGGCCR	5 cut(s)	84, 315, 861, 1498, 1562
AcyI	GRCGYC	1 cut(s)	1408
AfaI	GTAC	8 cut(s)	8, 472, 553, 884, 915, 1291, 1517, 1625
AfeI	AGCGCT	1 cut(s)	505
AfiI	CCNNNNNNNGG	3 cut(s)	625, 713, 1596
AflIII	ACRYGT	1 cut(s)	1249
AgsI	TTSAA	4 cut(s)	490, 589, 731, 1054
AhlI	ACTAGT	2 cut(s)	76, 554
AjnI	CCWGG	5 cut(s)	784, 1580, 1639, 1717, 1774
AleI	CACNNNNGTG	1 cut(s)	1343
Alw21I	GWGCWC	3 cut(s)	420, 596, 1111
Alw26I	GTCTC	3 cut(s)	518, 634, 1011
Ama87I	CYCGRG	1 cut(s)	1519
Aor51HI	AGCGCT	1 cut(s)	505
ApeKI	GCWGC	5 cut(s)	132, 609, 809, 1316, 1696
ArsI	GACNNNNNNTTYG	2 cut(s)	1289, 1321
AseI	ATTAAT	1 cut(s)	68
Asp718I	GGTACC	1 cut(s)	1289
AspLEI	GCGC	3 cut(s)	506, 1353, 1410
AspS9I	GGNCC	7 cut(s)	355, 373, 901, 1099, 1151, 1524, 1637
AsuHPI	GGTGA	5 cut(s)	578, 1033, 1195, 1322, 1748
AsuNHI	GCTAGC	2 cut(s)	365, 1646
AvaI	CYCGRG	1 cut(s)	1519
AvaII	GGWCC	4 cut(s)	355, 1099, 1151, 1524
BalI	TGGCCA	1 cut(s)	863
BanI	GGYRCC	3 cut(s)	1067, 1289, 1407
BanII	GRGCYC	4 cut(s)	420, 596, 1111, 1602
BauI	CACGAG	1 cut(s)	719
Bbv12I	GWGCWC	3 cut(s)	420, 596, 1111
BbvI	GCAGC	5 cut(s)	144, 621, 796, 1303, 1708
BccI	CCATC	7 cut(s)	692, 863, 899, 1217, 1476, 1535, 1737
BceAI	ACGGC	3 cut(s)	145, 1081, 1485
BciT130I	CCWGG	5 cut(s)	786, 1582, 1641, 1719, 1776
BclI	TGATCA	1 cut(s)	477
BcoDI	GTCTC	3 cut(s)	518, 634, 1011
BcuI	ACTAGT	2 cut(s)	76, 554
BfaI	CTAG	6 cut(s)	77, 360, 366, 555, 813, 1647
BfmI	CTRYAG	2 cut(s)	1089, 1207
BfoI	RGCGCY	2 cut(s)	507, 1411
BfuAI	ACCTGC	1 cut(s)	1713
BglI	GCCNNNNNGGC	1 cut(s)	1196
BmcAI	AGTACT	2 cut(s)	553, 1517
Bme1390I	CCNGG	5 cut(s)	786, 1582, 1641, 1719, 1776
Bme18I	GGWCC	4 cut(s)	355, 1099, 1151, 1524
BmeT110I	CYCGRG	1 cut(s)	1519
BmgT120I	GGNCC	7 cut(s)	355, 373, 901, 1099, 1151, 1524, 1637
BmiI	GGNNCC	4 cut(s)	357, 1069, 1291, 1409
BmrFI	CCNGG	5 cut(s)	786, 1582, 1641, 1719, 1776
BmsI	GCATC	6 cut(s)	60, 251, 996, 1303, 1444, 1686
BmtI	GCTAGC	2 cut(s)	369, 1650
BoxI	GACNNNNGTC	2 cut(s)	143, 938
BpmI	CTGGAG	1 cut(s)	1779
Bpu10I	CCTNAGC	3 cut(s)	674, 918, 1122
BpuEI	CTTGAG	4 cut(s)	368, 404, 1374, 1586
Bsa29I	ATCGAT	1 cut(s)	1741
BsaBI	GATNNNNATC	1 cut(s)	1010
BsaHI	GRCGYC	1 cut(s)	1408
BsaI	GGTCTC	1 cut(s)	634
BsaJI	CCNNGG	9 cut(s)	312, 624, 864, 987, 1102, 1505, 1580, 1581, 1639
BsaWI	WCCGGW	2 cut(s)	1043, 1286
Bsc4I	CCNNNNNNNGG	3 cut(s)	625, 713, 1596
Bse118I	RCCGGY	1 cut(s)	1148
Bse1I	ACTGG	2 cut(s)	1369, 1423
Bse3DI	GCAATG	2 cut(s)	303, 1672
Bse8I	GATNNNNATC	1 cut(s)	1010
BseBI	CCWGG	5 cut(s)	786, 1582, 1641, 1719, 1776
BseCI	ATCGAT	1 cut(s)	1741
BseDI	CCNNGG	9 cut(s)	312, 624, 864, 987, 1102, 1505, 1580, 1581, 1639
BseGI	GGATG	2 cut(s)	700, 874
BseJI	GATNNNNATC	1 cut(s)	1010
BseLI	CCNNNNNNNGG	3 cut(s)	625, 713, 1596
BseMI	GCAATG	2 cut(s)	303, 1672
BseMII	CTCAG	4 cut(s)	664, 1158, 1463, 1611
BseNI	ACTGG	2 cut(s)	1369, 1423
BseXI	GCAGC	5 cut(s)	144, 621, 796, 1303, 1708
BsgI	GTGCAG	2 cut(s)	1261, 1321
Bsh1236I	CGCG	5 cut(s)	89, 320, 848, 1097, 1272
BshNI	GGYRCC	3 cut(s)	1067, 1289, 1407
BshVI	ATCGAT	1 cut(s)	1741
BsiHKAI	GWGCWC	3 cut(s)	420, 596, 1111
BsiHKCI	CYCGRG	1 cut(s)	1519
BsiSI	CCGG	4 cut(s)	1044, 1149, 1287, 1396
BsiWI	CGTACG	1 cut(s)	6
BslI	CCNNNNNNNGG	3 cut(s)	625, 713, 1596
BsmAI	GTCTC	3 cut(s)	518, 634, 1011
BsmBI	CGTCTC	1 cut(s)	1011
Bso31I	GGTCTC	1 cut(s)	634
BsoBI	CYCGRG	1 cut(s)	1519
Bsp1286I	GDGCHC	4 cut(s)	420, 596, 1111, 1602
Bsp143I	GATC	4 cut(s)	477, 760, 1011, 1738
Bsp19I	CCATGG	3 cut(s)	624, 1102, 1505
BspCNI	CTCAG	4 cut(s)	663, 1157, 1464, 1612
BspDI	ATCGAT	1 cut(s)	1741
BspFNI	CGCG	5 cut(s)	89, 320, 848, 1097, 1272
BspLI	GGNNCC	4 cut(s)	357, 1069, 1291, 1409
BspMI	ACCTGC	1 cut(s)	1713
BspOI	GCTAGC	2 cut(s)	369, 1650
BspQI	GCTCTTC	1 cut(s)	584
BspT107I	GGYRCC	3 cut(s)	1067, 1289, 1407
BspTNI	GGTCTC	1 cut(s)	634
BsrDI	GCAATG	2 cut(s)	303, 1672
BsrFI	RCCGGY	1 cut(s)	1148
BsrI	ACTGG	2 cut(s)	1369, 1423
BssAI	RCCGGY	1 cut(s)	1148
BssECI	CCNNGG	9 cut(s)	312, 624, 864, 987, 1102, 1505, 1580, 1581, 1639
BssMI	GATC	4 cut(s)	477, 760, 1011, 1738
BssNAI	GTATAC	1 cut(s)	829
BssNI	GRCGYC	1 cut(s)	1408
BssSI	CACGAG	1 cut(s)	719
BssT1I	CCWWGG	5 cut(s)	624, 864, 987, 1102, 1505
Bst1107I	GTATAC	1 cut(s)	829
Bst2BI	CACGAG	1 cut(s)	719
Bst2UI	CCWGG	5 cut(s)	786, 1582, 1641, 1719, 1776
Bst4CI	ACNGT	4 cut(s)	94, 511, 1022, 1093
Bst6I	CTCTTC	3 cut(s)	61, 584, 646
BstACI	GRCGYC	1 cut(s)	1408
BstC8I	GCNNGC	9 cut(s)	39, 197, 367, 426, 683, 1355, 1412, 1648, 1701
BstDEI	CTNAG	8 cut(s)	650, 658, 674, 918, 1122, 1144, 1472, 1620
BstDSI	CCRYGG	4 cut(s)	312, 624, 1102, 1505
BstEII	GGTNACC	1 cut(s)	1575
BstF5I	GGATG	2 cut(s)	700, 874
BstFNI	CGCG	5 cut(s)	89, 320, 848, 1097, 1272
BstH2I	RGCGCY	2 cut(s)	507, 1411
BstHHI	GCGC	3 cut(s)	506, 1353, 1410
BstKTI	GATC	4 cut(s)	480, 763, 1014, 1741
BstMAI	GTCTC	3 cut(s)	518, 634, 1011
BstMBI	GATC	4 cut(s)	477, 760, 1011, 1738
BstMWI	GCNNNNNNNGC	6 cut(s)	371, 678, 818, 1094, 1196, 1652
BstNI	CCWGG	5 cut(s)	786, 1582, 1641, 1719, 1776
BstNSI	RCATGY	5 cut(s)	387, 531, 1253, 1611, 1703
BstPAI	GACNNNNGTC	2 cut(s)	143, 938
BstPI	GGTNACC	1 cut(s)	1575
BstSCI	CCNGG	5 cut(s)	784, 1580, 1639, 1717, 1774
BstSFI	CTRYAG	2 cut(s)	1089, 1207
BstUI	CGCG	5 cut(s)	89, 320, 848, 1097, 1272
BstV1I	GCAGC	5 cut(s)	144, 621, 796, 1303, 1708
BstXI	CCANNNNNNTGG	3 cut(s)	1418, 1534, 1572
BstZ17I	GTATAC	1 cut(s)	829
Bsu15I	ATCGAT	1 cut(s)	1741
BsuTUI	ATCGAT	1 cut(s)	1741
BtgI	CCRYGG	4 cut(s)	312, 624, 1102, 1505
BtsCI	GGATG	2 cut(s)	700, 874
BtsI	GCAGTG	1 cut(s)	1782
BtsIMutI	CAGTG	3 cut(s)	459, 658, 1782
BveI	ACCTGC	1 cut(s)	1713
Cac8I	GCNNGC	9 cut(s)	39, 197, 367, 426, 683, 1355, 1412, 1648, 1701
CfoI	GCGC	3 cut(s)	506, 1353, 1410
Cfr10I	RCCGGY	1 cut(s)	1148
Cfr13I	GGNCC	7 cut(s)	355, 373, 901, 1099, 1151, 1524, 1637
ClaI	ATCGAT	1 cut(s)	1741
CseI	GACGC	1 cut(s)	545
Csp6I	GTAC	8 cut(s)	7, 471, 552, 883, 914, 1290, 1516, 1624
CspCI	CAANNNNNGTGG	2 cut(s)	1615, 1650
CviQI	GTAC	8 cut(s)	7, 471, 552, 883, 914, 1290, 1516, 1624
DdeI	CTNAG	8 cut(s)	650, 658, 674, 918, 1122, 1144, 1472, 1620
DinI	GGCGCC	1 cut(s)	1409
DpnI	GATC	4 cut(s)	479, 762, 1013, 1740
DpnII	GATC	4 cut(s)	477, 760, 1011, 1738
EaeI	YGGCCR	5 cut(s)	84, 315, 861, 1498, 1562
Eam1104I	CTCTTC	3 cut(s)	61, 584, 646
EarI	CTCTTC	3 cut(s)	61, 584, 646
Ecl136II	GAGCTC	3 cut(s)	418, 594, 1109
Eco130I	CCWWGG	5 cut(s)	624, 864, 987, 1102, 1505
Eco147I	AGGCCT	1 cut(s)	672
Eco24I	GRGCYC	4 cut(s)	420, 596, 1111, 1602
Eco31I	GGTCTC	1 cut(s)	634
Eco32I	GATATC	1 cut(s)	162
Eco47I	GGWCC	4 cut(s)	355, 1099, 1151, 1524
Eco47III	AGCGCT	1 cut(s)	505
Eco53kI	GAGCTC	3 cut(s)	418, 594, 1109
Eco88I	CYCGRG	1 cut(s)	1519
Eco91I	GGTNACC	1 cut(s)	1575
EcoICRI	GAGCTC	3 cut(s)	418, 594, 1109
EcoO109I	RGGNCCY	3 cut(s)	355, 373, 901
EcoO65I	GGTNACC	1 cut(s)	1575
EcoRII	CCWGG	5 cut(s)	784, 1580, 1639, 1717, 1774
EcoRV	GATATC	1 cut(s)	162
EcoT14I	CCWWGG	5 cut(s)	624, 864, 987, 1102, 1505
EcoT22I	ATGCAT	2 cut(s)	603, 1705
EcoT38I	GRGCYC	4 cut(s)	420, 596, 1111, 1602
EgeI	GGCGCC	1 cut(s)	1409
EheI	GGCGCC	1 cut(s)	1409
ErhI	CCWWGG	5 cut(s)	624, 864, 987, 1102, 1505
Esp3I	CGTCTC	1 cut(s)	1011
FalI	AAGNNNNNCTT	4 cut(s)	1224, 1256, 1595, 1627
FbaI	TGATCA	1 cut(s)	477
FblI	GTMKAC	3 cut(s)	828, 963, 1062
FokI	GGATG	2 cut(s)	707, 881
FriOI	GRGCYC	4 cut(s)	420, 596, 1111, 1602
FspBI	CTAG	6 cut(s)	77, 360, 366, 555, 813, 1647
FspI	TGCGCA	1 cut(s)	1352
GlaI	GCGC	3 cut(s)	505, 1352, 1409
GsuI	CTGGAG	1 cut(s)	1779
HaeII	RGCGCY	2 cut(s)	507, 1411
HapII	CCGG	4 cut(s)	1044, 1149, 1287, 1396
HgaI	GACGC	1 cut(s)	545
HhaI	GCGC	3 cut(s)	506, 1353, 1410
Hin1I	GRCGYC	1 cut(s)	1408
Hin6I	GCGC	3 cut(s)	504, 1351, 1408
HinP1I	GCGC	3 cut(s)	504, 1351, 1408
HindIII	AAGCTT	1 cut(s)	426
HinfI	GANTC	5 cut(s)	14, 285, 1038, 1422, 1538
HpaII	CCGG	4 cut(s)	1044, 1149, 1287, 1396
HphI	GGTGA	5 cut(s)	578, 1033, 1195, 1322, 1748
Hpy166II	GTNNAC	4 cut(s)	829, 964, 979, 1063
Hpy188I	TCNGA	4 cut(s)	1005, 1016, 1453, 1473
Hpy188III	TCNNGA	8 cut(s)	246, 496, 719, 737, 1154, 1603, 1681, 1758
Hpy8I	GTNNAC	4 cut(s)	829, 964, 979, 1063
HpyAV	CCTTC	3 cut(s)	346, 950, 1504
HpyCH4III	ACNGT	4 cut(s)	94, 511, 1022, 1093
HpyCH4IV	ACGT	1 cut(s)	825
HpyF10VI	GCNNNNNNNGC	6 cut(s)	371, 678, 818, 1094, 1196, 1652
HpyF3I	CTNAG	8 cut(s)	650, 658, 674, 918, 1122, 1144, 1472, 1620
HpySE526I	ACGT	1 cut(s)	825
Hsp92I	GRCGYC	1 cut(s)	1408
HspAI	GCGC	3 cut(s)	504, 1351, 1408
KasI	GGCGCC	1 cut(s)	1407
KpnI	GGTACC	1 cut(s)	1293
Ksp22I	TGATCA	1 cut(s)	477
Kzo9I	GATC	4 cut(s)	477, 760, 1011, 1738
LguI	GCTCTTC	1 cut(s)	584
LmnI	GCTCC	3 cut(s)	599, 690, 1106
Lsp1109I	GCAGC	5 cut(s)	144, 621, 796, 1303, 1708
LweI	GCATC	6 cut(s)	60, 251, 996, 1303, 1444, 1686
MaeI	CTAG	6 cut(s)	77, 360, 366, 555, 813, 1647
MaeII	ACGT	1 cut(s)	825
MaeIII	GTNAC	8 cut(s)	150, 409, 446, 511, 716, 1381, 1575, 1663
MalI	GATC	4 cut(s)	479, 762, 1013, 1740
MboI	GATC	4 cut(s)	477, 760, 1011, 1738
MboII	GAAGA	7 cut(s)	48, 334, 583, 589, 601, 633, 1195
MhlI	GDGCHC	4 cut(s)	420, 596, 1111, 1602
MlsI	TGGCCA	1 cut(s)	863
MluCI	AATT	6 cut(s)	183, 205, 465, 613, 1176, 1262
MluNI	TGGCCA	1 cut(s)	863
Mly113I	GGCGCC	1 cut(s)	1408
MlyI	GAGTC	1 cut(s)	1547
MmeI	TCCRAC	2 cut(s)	262, 1279
Mox20I	TGGCCA	1 cut(s)	863
Mph1103I	ATGCAT	2 cut(s)	603, 1705
MscI	TGGCCA	1 cut(s)	863
MseI	TTAA	6 cut(s)	68, 747, 756, 765, 837, 1731
MslI	CAYNNNNRTG	3 cut(s)	857, 1343, 1532
Msp20I	TGGCCA	1 cut(s)	863
MspI	CCGG	4 cut(s)	1044, 1149, 1287, 1396
MspR9I	CCNGG	5 cut(s)	786, 1582, 1641, 1719, 1776
MvaI	CCWGG	5 cut(s)	786, 1582, 1641, 1719, 1776
MvnI	CGCG	5 cut(s)	89, 320, 848, 1097, 1272
MwoI	GCNNNNNNNGC	6 cut(s)	371, 678, 818, 1094, 1196, 1652
NarI	GGCGCC	1 cut(s)	1408
NcoI	CCATGG	3 cut(s)	624, 1102, 1505
NdeII	GATC	4 cut(s)	477, 760, 1011, 1738
NheI	GCTAGC	2 cut(s)	365, 1646
NlaIV	GGNNCC	4 cut(s)	357, 1069, 1291, 1409
NmuCI	GTSAC	6 cut(s)	150, 446, 511, 716, 1381, 1663
NsbI	TGCGCA	1 cut(s)	1352
NsiI	ATGCAT	2 cut(s)	603, 1705
NspI	RCATGY	5 cut(s)	387, 531, 1253, 1611, 1703
OliI	CACNNNNGTG	1 cut(s)	1343
PaeI	GCATGC	1 cut(s)	1703
PaeR7I	CTCGAG	1 cut(s)	1519
PasI	CCCWGGG	1 cut(s)	1581
PceI	AGGCCT	1 cut(s)	672
PciI	ACATGT	1 cut(s)	1249
PciSI	GCTCTTC	1 cut(s)	584
PfeI	GAWTC	4 cut(s)	14, 285, 1038, 1422
Pfl23II	CGTACG	1 cut(s)	6
PleI	GAGTC	1 cut(s)	1546
PluTI	GGCGCC	1 cut(s)	1411
PpsI	GAGTC	1 cut(s)	1546
PpuMI	RGGWCCY	1 cut(s)	355
PscI	ACATGT	1 cut(s)	1249
PshAI	GACNNNNGTC	2 cut(s)	143, 938
PshBI	ATTAAT	1 cut(s)	68
PsiI	TTATAA	1 cut(s)	1433
Psp124BI	GAGCTC	3 cut(s)	420, 596, 1111
Psp5II	RGGWCCY	1 cut(s)	355
Psp6I	CCWGG	5 cut(s)	784, 1580, 1639, 1717, 1774
PspEI	GGTNACC	1 cut(s)	1575
PspGI	CCWGG	5 cut(s)	784, 1580, 1639, 1717, 1774
PspLI	CGTACG	1 cut(s)	6
PspN4I	GGNNCC	4 cut(s)	357, 1069, 1291, 1409
PspPI	GGNCC	7 cut(s)	355, 373, 901, 1099, 1151, 1524, 1637
PspPPI	RGGWCCY	1 cut(s)	355
PspXI	VCTCGAGB	1 cut(s)	1519
RsaI	GTAC	8 cut(s)	8, 472, 553, 884, 915, 1291, 1517, 1625
RsaNI	GTAC	8 cut(s)	7, 471, 552, 883, 914, 1290, 1516, 1624
RseI	CAYNNNNRTG	3 cut(s)	857, 1343, 1532
SacI	GAGCTC	3 cut(s)	420, 596, 1111
SapI	GCTCTTC	1 cut(s)	584
SaqAI	TTAA	6 cut(s)	68, 747, 756, 765, 837, 1731
Sau3AI	GATC	4 cut(s)	477, 760, 1011, 1738
Sau96I	GGNCC	7 cut(s)	355, 373, 901, 1099, 1151, 1524, 1637
ScaI	AGTACT	2 cut(s)	553, 1517
SchI	GAGTC	1 cut(s)	1547
ScrFI	CCNGG	5 cut(s)	786, 1582, 1641, 1719, 1776
SduI	GDGCHC	4 cut(s)	420, 596, 1111, 1602
SfaNI	GCATC	6 cut(s)	60, 251, 996, 1303, 1444, 1686
SfcI	CTRYAG	2 cut(s)	1089, 1207
SfoI	GGCGCC	1 cut(s)	1409
Sfr274I	CTCGAG	1 cut(s)	1519
SinI	GGWCC	4 cut(s)	355, 1099, 1151, 1524
SlaI	CTCGAG	1 cut(s)	1519
SmiMI	CAYNNNNRTG	3 cut(s)	857, 1343, 1532
SmlI	CTYRAG	5 cut(s)	347, 419, 1389, 1519, 1601
SmoI	CTYRAG	5 cut(s)	347, 419, 1389, 1519, 1601
SpeI	ACTAGT	2 cut(s)	76, 554
SphI	GCATGC	1 cut(s)	1703
Sse9I	AATT	6 cut(s)	183, 205, 465, 613, 1176, 1262
SseBI	AGGCCT	1 cut(s)	672
SspDI	GGCGCC	1 cut(s)	1407
SspMI	CTAG	6 cut(s)	77, 360, 366, 555, 813, 1647
SstI	GAGCTC	3 cut(s)	420, 596, 1111
StuI	AGGCCT	1 cut(s)	672
StyD4I	CCNGG	5 cut(s)	784, 1580, 1639, 1717, 1774
StyI	CCWWGG	5 cut(s)	624, 864, 987, 1102, 1505
TaaI	ACNGT	4 cut(s)	94, 511, 1022, 1093
TaiI	ACGT	1 cut(s)	828
TaqI	TCGA	4 cut(s)	59, 432, 1520, 1741
TasI	AATT	6 cut(s)	183, 205, 465, 613, 1176, 1262
TatI	WGTACW	4 cut(s)	551, 882, 1515, 1623
TauI	GCSGC	7 cut(s)	89, 320, 681, 848, 1322, 1460, 1696
TfiI	GAWTC	4 cut(s)	14, 285, 1038, 1422
Tru1I	TTAA	6 cut(s)	68, 747, 756, 765, 837, 1731
Tru9I	TTAA	6 cut(s)	68, 747, 756, 765, 837, 1731
TscAI	CASTG	3 cut(s)	466, 658, 1789
TseFI	GTSAC	6 cut(s)	150, 446, 511, 716, 1381, 1663
TseI	GCWGC	5 cut(s)	132, 609, 809, 1316, 1696
Tsp45I	GTSAC	6 cut(s)	150, 446, 511, 716, 1381, 1663
TspDTI	ATGAA	7 cut(s)	188, 314, 590, 633, 1105, 1494, 1546
TspGWI	ACGGA	1 cut(s)	301
TspRI	CASTG	3 cut(s)	466, 658, 1789
VpaK11BI	GGWCC	4 cut(s)	355, 1099, 1151, 1524
VspI	ATTAAT	1 cut(s)	68
XceI	RCATGY	5 cut(s)	387, 531, 1253, 1611, 1703
XhoI	CTCGAG	1 cut(s)	1519
XmiI	GTMKAC	3 cut(s)	828, 963, 1062
XspI	CTAG	6 cut(s)	77, 360, 366, 555, 813, 1647
ZrmI	AGTACT	2 cut(s)	553, 1517
Zsp2I	ATGCAT	2 cut(s)	603, 1705

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.