Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Forward (+)

12980937 .. 12983173

Gene Structure 2237 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1164500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGCAGCTAGTGAATCAAAGAAGAAGAAGATAGCCATCATCGCCGTATTGGTTGTACTTCTGGTGGCGATGATAGTGGCGGTGACGGTGGGTGTCAAAATGGCCAAAAAGGGCAACAAGAAGCCTGCCAAGGTTGGTGCGAAGAAAAAGGCCATCGAGACCATATGCCAGGCCACCACCTACAAAGAAACCTGCCAAAGCAGCCTCGAAAAGGAGACGGGTGACACCACCGACCCGAAGGACCTGATCAAGGCCGAGTTCCAGGTGGCGGCGGACAAAGTTAATGAGGTGATCAAGAACTCGAACACCTTGAAAGAGTATGCCAAGGATCCAATGGCCAGCCAGGCTCTGGGCGTGTGCAAGGAATTGTTGGAAACCGCAGTTGAAGATCTCCTCGGGGCGGTTGATAAGATGGGGCCATTCGATGTTAGCAAGATCAGTGACTACCTTGCAGATCTCAAGGTGTGGCTGAGCGCCGCCGTCAATCACCAGGCAACCTGCCTTGACGCGTTTGAGGACACCAAAGGCGAGGCCGGCGAGAAAATGAGGGGCTTTTTGAAGACAGCGCAAGAACTCACCAGCAATGGACTTGCCATGGTCTCGGAAATCTCTCGTCTCGTCGATTCTCCTAAATTGAACTCTCACCGTCGCCTCATGTCCTCGTCAAAAGGCGGGCAGACAAAGAGGAATTATAAGCCAGTGCCAGGGTGGATGAGTGATCGCCATCTGGATCTCTTGACTGCCACTCCGGAGAGCTTGAAGCCCAACGTGGTTGTGGCTAAGGATGGGACTGGCAAGTATAAGACCATCAACGAGGCCTTGAAGGAGGTCCCACAAAAGAGCAACGAGGCCTTTGTGATTTATATTAAGAAGGGAATGTACGAAGAGACTGTCATCGTTGACAAGGAAATGACCAATGTTGTGATGATTGGTGACGGTCCAACCATGACCAGGATCAGCGGAAGCAAGAGCCACGCGGATGGTATCACAACCATGAACACTGCAACCTTTGCCGCAGTTGGAAACTATTTCGTTGCCAAGGGAATTGGGTTCGAGAACACAGCAGGACACGTAAAGCACCAAGCCGTGGCACTGCGTGTGCAATCCGACTTCTCCATCTTCTTCAACTGCCACATGGATGCATACCAAGACACACTCTACACCCAAACCTACCGCCAATTCTACCGCGACTGCACCATCACTGGTACAGTAGACTTCATCTTTGGCGATGGGGTGGCTTTGTTCCAAAACTGCAAGATGGTGGTGAGAAAGCCAGGGGAAGCACAGGCTTGCATGGTGACAGCACAAGGCAGGACTGACCGCAACGAGATCACCGGCATTTTCCTCCAAAACTGCACCATCACCGGTGAGCCAGATTACATGGCTGTGAAGGATAAGAACAAGGCATACTTGGGGAGGCCATGGAAGAAATTTGCAAGGGTAGTTGTGATGCAGTCATCGATTGACGAAGCCATTGCACCTGAAGGGTGGACAGAATGGACTGGTGAAAAATACCACCAAAACTGCTTCTTTGGTGAGTACGGAAACAGGGGTCCTAGGAAGGACATGACCCAAAGGGTCAAATGGTCAACCGTCAAGAGACTCACTCCTGATCAAGCTGCGGCTTTCACCCCTGCTAGGGTGTTTGAGGGTGATAAGTGGGTAAAGCCCAGTGGGGTTCCATATGTTCCAGGCATGATGCCCGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

570

Amino Acids

62.71

Weight (kDa)

8.96

Isoelectric Point (pI)

26.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	50 - 199	2.9e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	256 - 552	1.5e-128	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	693
Acc36I	ACCTGC	2 cut(s)	200, 506
AccB7I	CCANNNNNTGG	2 cut(s)	349, 1087
AccI	GTMKAC	1 cut(s)	1212
AccII	CGCG	3 cut(s)	509, 977, 1188
AccIII	TCCGGA	1 cut(s)	748
AclWI	GGATC	4 cut(s)	323, 336, 738, 962
AcoI	YGGCCR	2 cut(s)	102, 336
AcsI	RAATTY	1 cut(s)	1430
AcuI	CTGAAG	1 cut(s)	1503
AdeI	CACNNNGTG	1 cut(s)	1097
AfaI	GTAC	4 cut(s)	57, 881, 1207, 1541
AflIII	ACRYGT	2 cut(s)	507, 1069
AgeI	ACCGGT	1 cut(s)	1364
AgsI	TTSAA	7 cut(s)	313, 386, 559, 637, 760, 823, 1126
AjnI	CCWGG	8 cut(s)	168, 261, 342, 489, 703, 950, 1273, 1690
AluBI	AGCT	3 cut(s)	8, 756, 1619
AluI	AGCT	3 cut(s)	8, 756, 1619
Alw26I	GTCTC	6 cut(s)	152, 209, 604, 620, 881, 1594
AlwI	GGATC	4 cut(s)	323, 336, 738, 962
AlwNI	CAGNNNCTG	1 cut(s)	349
Ama87I	CYCGRG	1 cut(s)	395
Aor13HI	TCCGGA	1 cut(s)	748
ApeKI	GCWGC	3 cut(s)	5, 201, 1619
ApoI	RAATTY	1 cut(s)	1430
ArsI	GACNNNNNNTTYG	4 cut(s)	876, 908, 1205, 1237
AsiGI	ACCGGT	1 cut(s)	1364
AspA2I	CCTAGG	1 cut(s)	1556
AspLEI	GCGC	2 cut(s)	476, 568
AspS9I	GGNCC	5 cut(s)	241, 416, 829, 938, 1553
AvaI	CYCGRG	1 cut(s)	395
AvaII	GGWCC	4 cut(s)	241, 829, 938, 1553
AvrII	CCTAGG	1 cut(s)	1556
BalI	TGGCCA	2 cut(s)	104, 338
BamHI	GGATCC	1 cut(s)	328
BarI	GAAGNNNNNNTAC	2 cut(s)	863, 895
BbsI	GAAGAC	1 cut(s)	566
BbvI	GCAGC	3 cut(s)	17, 213, 1606
BceAI	ACGGC	3 cut(s)	29, 464, 1070
BcgI	CGANNNNNNTGC	2 cut(s)	1457, 1491
BciT130I	CCWGG	8 cut(s)	170, 263, 344, 491, 705, 952, 1275, 1692
BclI	TGATCA	3 cut(s)	246, 291, 1612
BcoDI	GTCTC	6 cut(s)	152, 209, 604, 620, 881, 1594
BfaI	CTAG	3 cut(s)	9, 1557, 1638
BfoI	RGCGCY	1 cut(s)	477
BfuAI	ACCTGC	2 cut(s)	200, 506
BglI	GCCNNNNNGGC	1 cut(s)	344
BglII	AGATCT	2 cut(s)	388, 454
BisI	GCNGC	7 cut(s)	6, 202, 270, 477, 1014, 1620, 1623
BlnI	CCTAGG	1 cut(s)	1556
BlpI	GCTNAGC	1 cut(s)	470
BlsI	GCNGC	7 cut(s)	7, 203, 271, 478, 1015, 1621, 1624
Bme1390I	CCNGG	8 cut(s)	170, 263, 344, 491, 705, 952, 1275, 1692
Bme18I	GGWCC	4 cut(s)	241, 829, 938, 1553
BmeT110I	CYCGRG	1 cut(s)	395
BmgT120I	GGNCC	5 cut(s)	241, 416, 829, 938, 1553
BmiI	GGNNCC	5 cut(s)	330, 417, 831, 1554, 1680
BmrFI	CCNGG	8 cut(s)	170, 263, 344, 491, 705, 952, 1275, 1692
BmrI	ACTGGG	1 cut(s)	1665
BmsI	GCATC	3 cut(s)	1129, 1440, 1689
BmuI	ACTGGG	1 cut(s)	1665
BpiI	GAAGAC	1 cut(s)	566
BplI	GAGNNNNNCTC	2 cut(s)	1591, 1623
Bpu10I	CCTNAGC	1 cut(s)	780
Bpu1102I	GCTNAGC	1 cut(s)	470
BpuEI	CTTGAG	1 cut(s)	443
Bsa29I	ATCGAT	1 cut(s)	1460
BsaAI	YACGTR	1 cut(s)	1072
BsaBI	GATNNNNATC	2 cut(s)	35, 723
BsaI	GGTCTC	2 cut(s)	152, 604
BsaWI	WCCGGW	2 cut(s)	748, 1364
BsaXI	ACNNNNNCTCC	2 cut(s)	730, 760
Bse118I	RCCGGY	3 cut(s)	533, 1334, 1364
Bse1I	ACTGG	5 cut(s)	698, 796, 1207, 1507, 1671
Bse3DI	GCAATG	2 cut(s)	589, 1473
Bse8I	GATNNNNATC	2 cut(s)	35, 723
BseAI	TCCGGA	1 cut(s)	748
BseBI	CCWGG	8 cut(s)	170, 263, 344, 491, 705, 952, 1275, 1692
BseCI	ATCGAT	1 cut(s)	1460
BseGI	GGATG	4 cut(s)	717, 790, 985, 1144
BseJI	GATNNNNATC	2 cut(s)	35, 723
BseMI	GCAATG	2 cut(s)	589, 1473
BseMII	CTCAG	1 cut(s)	461
BseNI	ACTGG	5 cut(s)	698, 796, 1207, 1507, 1671
BseRI	GAGGAG	1 cut(s)	383
BseXI	GCAGC	3 cut(s)	17, 213, 1606
BsgI	GTGCAG	2 cut(s)	1177, 1339
Bsh1236I	CGCG	3 cut(s)	509, 977, 1188
BshTI	ACCGGT	1 cut(s)	1364
BshVI	ATCGAT	1 cut(s)	1460
BsiHKCI	CYCGRG	1 cut(s)	395
BsiSI	CCGG	4 cut(s)	534, 749, 1335, 1365
BslFI	GGGAC	2 cut(s)	802, 815
BsmAI	GTCTC	6 cut(s)	152, 209, 604, 620, 881, 1594
BsmBI	CGTCTC	2 cut(s)	209, 620
BsmFI	GGGAC	2 cut(s)	802, 815
Bso31I	GGTCTC	2 cut(s)	152, 604
BsoBI	CYCGRG	1 cut(s)	395
Bsp13I	TCCGGA	1 cut(s)	748
Bsp1720I	GCTNAGC	1 cut(s)	470
Bsp19I	CCATGG	2 cut(s)	594, 1421
BspCNI	CTCAG	1 cut(s)	462
BspDI	ATCGAT	1 cut(s)	1460
BspEI	TCCGGA	1 cut(s)	748
BspFNI	CGCG	3 cut(s)	509, 977, 1188
BspLI	GGNNCC	5 cut(s)	330, 417, 831, 1554, 1680
BspMI	ACCTGC	2 cut(s)	200, 506
BspPI	GGATC	4 cut(s)	323, 336, 738, 962
BspTNI	GGTCTC	2 cut(s)	152, 604
BsrDI	GCAATG	2 cut(s)	589, 1473
BsrFI	RCCGGY	3 cut(s)	533, 1334, 1364
BsrI	ACTGG	5 cut(s)	698, 796, 1207, 1507, 1671
BssAI	RCCGGY	3 cut(s)	533, 1334, 1364
BssT1I	CCWWGG	6 cut(s)	129, 324, 594, 1038, 1421, 1556
Bst2UI	CCWGG	8 cut(s)	170, 263, 344, 491, 705, 952, 1275, 1692
Bst4CI	ACNGT	6 cut(s)	88, 647, 892, 938, 1210, 1594
Bst6I	CTCTTC	1 cut(s)	879
BstAPI	GCANNNNNTGC	1 cut(s)	1010
BstBAI	YACGTR	1 cut(s)	1072
BstC8I	GCNNGC	5 cut(s)	126, 340, 535, 674, 1291
BstDEI	CTNAG	2 cut(s)	470, 780
BstDSI	CCRYGG	3 cut(s)	594, 1086, 1421
BstENI	CCTNNNNNAGG	2 cut(s)	209, 248
BstF5I	GGATG	4 cut(s)	717, 790, 985, 1144
BstFNI	CGCG	3 cut(s)	509, 977, 1188
BstH2I	RGCGCY	1 cut(s)	477
BstHHI	GCGC	2 cut(s)	476, 568
BstMAI	GTCTC	6 cut(s)	152, 209, 604, 620, 881, 1594
BstMWI	GCNNNNNNNGC	5 cut(s)	41, 201, 344, 534, 1010
BstNI	CCWGG	8 cut(s)	170, 263, 344, 491, 705, 952, 1275, 1692
BstSCI	CCNGG	8 cut(s)	168, 261, 342, 489, 703, 950, 1273, 1690
BstUI	CGCG	3 cut(s)	509, 977, 1188
BstV1I	GCAGC	3 cut(s)	17, 213, 1606
BstV2I	GAAGAC	1 cut(s)	566
BstX2I	RGATCY	4 cut(s)	328, 388, 454, 730
BstXI	CCANNNNNNTGG	1 cut(s)	980
BstYI	RGATCY	4 cut(s)	328, 388, 454, 730
Bsu15I	ATCGAT	1 cut(s)	1460
BsuTUI	ATCGAT	1 cut(s)	1460
BtgI	CCRYGG	3 cut(s)	594, 1086, 1421
BtgZI	GCGATG	3 cut(s)	25, 83, 1242
BtsCI	GGATG	4 cut(s)	717, 790, 985, 1144
BtsI	GCAGTG	2 cut(s)	999, 1091
BtsIMutI	CAGTG	6 cut(s)	445, 705, 999, 1091, 1200, 1678
BveI	ACCTGC	2 cut(s)	200, 506
Cac8I	GCNNGC	5 cut(s)	126, 340, 535, 674, 1291
CaiI	CAGNNNCTG	1 cut(s)	349
CfoI	GCGC	2 cut(s)	476, 568
Cfr10I	RCCGGY	3 cut(s)	533, 1334, 1364
Cfr13I	GGNCC	5 cut(s)	241, 416, 829, 938, 1553
ClaI	ATCGAT	1 cut(s)	1460
CseI	GACGC	1 cut(s)	515
Csp6I	GTAC	4 cut(s)	56, 880, 1206, 1540
CspAI	ACCGGT	1 cut(s)	1364
CviQI	GTAC	4 cut(s)	56, 880, 1206, 1540
DdeI	CTNAG	2 cut(s)	470, 780
DraIII	CACNNNGTG	1 cut(s)	1097
EaeI	YGGCCR	2 cut(s)	102, 336
Eam1104I	CTCTTC	1 cut(s)	879
EarI	CTCTTC	1 cut(s)	879
EciI	GGCGGA	1 cut(s)	287
Eco130I	CCWWGG	6 cut(s)	129, 324, 594, 1038, 1421, 1556
Eco147I	AGGCCT	2 cut(s)	818, 851
Eco31I	GGTCTC	2 cut(s)	152, 604
Eco47I	GGWCC	4 cut(s)	241, 829, 938, 1553
Eco57I	CTGAAG	1 cut(s)	1503
Eco88I	CYCGRG	1 cut(s)	395
EcoNI	CCTNNNNNAGG	2 cut(s)	209, 248
EcoO109I	RGGNCCY	3 cut(s)	241, 829, 1553
EcoRII	CCWGG	8 cut(s)	168, 261, 342, 489, 703, 950, 1273, 1690
EcoT14I	CCWWGG	6 cut(s)	129, 324, 594, 1038, 1421, 1556
EcoT22I	ATGCAT	1 cut(s)	1144
ErhI	CCWWGG	6 cut(s)	129, 324, 594, 1038, 1421, 1556
Esp3I	CGTCTC	2 cut(s)	209, 620
FalI	AAGNNNNNCTT	2 cut(s)	1394, 1426
FaqI	GGGAC	2 cut(s)	802, 815
FauI	CCCGC	1 cut(s)	665
FauNDI	CATATG	2 cut(s)	164, 1684
FbaI	TGATCA	3 cut(s)	246, 291, 1612
FblI	GTMKAC	1 cut(s)	1212
Fnu4HI	GCNGC	7 cut(s)	6, 202, 270, 477, 1014, 1620, 1623
FokI	GGATG	4 cut(s)	724, 797, 992, 1151
Fsp4HI	GCNGC	7 cut(s)	6, 202, 270, 477, 1014, 1620, 1623
FspBI	CTAG	3 cut(s)	9, 1557, 1638
GlaI	GCGC	2 cut(s)	475, 567
GluI	GCNGC	7 cut(s)	6, 202, 270, 477, 1014, 1620, 1623
HaeII	RGCGCY	1 cut(s)	477
HapII	CCGG	4 cut(s)	534, 749, 1335, 1365
HgaI	GACGC	1 cut(s)	515
HhaI	GCGC	2 cut(s)	476, 568
Hin6I	GCGC	2 cut(s)	474, 566
HinP1I	GCGC	2 cut(s)	474, 566
HincII	GTYRAC	2 cut(s)	901, 1590
HindII	GTYRAC	2 cut(s)	901, 1590
HinfI	GANTC	3 cut(s)	14, 623, 1602
HpaII	CCGG	4 cut(s)	534, 749, 1335, 1365
Hpy166II	GTNNAC	4 cut(s)	901, 1213, 1491, 1590
Hpy188I	TCNGA	2 cut(s)	604, 1108
Hpy188III	TCNNGA	8 cut(s)	157, 295, 728, 736, 749, 1054, 1597, 1610
Hpy8I	GTNNAC	4 cut(s)	901, 1213, 1491, 1590
Hpy99I	CGWCG	2 cut(s)	623, 651
HpyAV	CCTTC	6 cut(s)	232, 817, 865, 1384, 1478, 1555
HpyCH4III	ACNGT	6 cut(s)	88, 647, 892, 938, 1210, 1594
HpyCH4IV	ACGT	2 cut(s)	768, 1071
HpyF10VI	GCNNNNNNNGC	5 cut(s)	41, 201, 344, 534, 1010
HpyF3I	CTNAG	2 cut(s)	470, 780
HpySE526I	ACGT	2 cut(s)	768, 1071
HspAI	GCGC	2 cut(s)	474, 566
Kpn2I	TCCGGA	1 cut(s)	748
KroI	GCCGGC	1 cut(s)	533
KroNI	GCCGGC	1 cut(s)	535
Ksp22I	TGATCA	3 cut(s)	246, 291, 1612
Lsp1109I	GCAGC	3 cut(s)	17, 213, 1606
LweI	GCATC	3 cut(s)	1129, 1440, 1689
MaeI	CTAG	3 cut(s)	9, 1557, 1638
MaeII	ACGT	2 cut(s)	768, 1071
MaeIII	GTNAC	5 cut(s)	82, 221, 440, 932, 1297
MflI	RGATCY	4 cut(s)	328, 388, 454, 730
MlsI	TGGCCA	2 cut(s)	104, 338
MluCI	AATT	6 cut(s)	365, 632, 688, 1044, 1178, 1430
MluI	ACGCGT	1 cut(s)	507
MluNI	TGGCCA	2 cut(s)	104, 338
MlyI	GAGTC	1 cut(s)	1596
MmeI	TCCRAC	4 cut(s)	351, 965, 1000, 1131
Mox20I	TGGCCA	2 cut(s)	104, 338
Mph1103I	ATGCAT	1 cut(s)	1144
MroI	TCCGGA	1 cut(s)	748
MroNI	GCCGGC	1 cut(s)	533
MscI	TGGCCA	2 cut(s)	104, 338
MseI	TTAA	2 cut(s)	282, 867
MslI	CAYNNNNRTG	4 cut(s)	978, 992, 1137, 1385
Msp20I	TGGCCA	2 cut(s)	104, 338
MspA1I	CMGCKG	1 cut(s)	960
MspI	CCGG	4 cut(s)	534, 749, 1335, 1365
MspR9I	CCNGG	8 cut(s)	170, 263, 344, 491, 705, 952, 1275, 1692
MvaI	CCWGG	8 cut(s)	170, 263, 344, 491, 705, 952, 1275, 1692
MvnI	CGCG	3 cut(s)	509, 977, 1188
MwoI	GCNNNNNNNGC	5 cut(s)	41, 201, 344, 534, 1010
NaeI	GCCGGC	1 cut(s)	535
NcoI	CCATGG	2 cut(s)	594, 1421
NdeI	CATATG	2 cut(s)	164, 1684
NgoMIV	GCCGGC	1 cut(s)	533
NlaIV	GGNNCC	5 cut(s)	330, 417, 831, 1554, 1680
NmeAIII	GCCGAG	1 cut(s)	280
NmuCI	GTSAC	5 cut(s)	82, 221, 440, 932, 1297
NsiI	ATGCAT	1 cut(s)	1144
PceI	AGGCCT	2 cut(s)	818, 851
PdiI	GCCGGC	1 cut(s)	535
PfeI	GAWTC	2 cut(s)	14, 623
PflMI	CCANNNNNTGG	2 cut(s)	349, 1087
PinAI	ACCGGT	1 cut(s)	1364
PkrI	GCNGC	7 cut(s)	7, 203, 271, 478, 1015, 1621, 1624
PleI	GAGTC	1 cut(s)	1596
PpsI	GAGTC	1 cut(s)	1596
Ppu21I	YACGTR	1 cut(s)	1072
PpuMI	RGGWCCY	3 cut(s)	241, 829, 1553
PsiI	TTATAA	1 cut(s)	693
Psp5II	RGGWCCY	3 cut(s)	241, 829, 1553
Psp6I	CCWGG	8 cut(s)	168, 261, 342, 489, 703, 950, 1273, 1690
PspGI	CCWGG	8 cut(s)	168, 261, 342, 489, 703, 950, 1273, 1690
PspN4I	GGNNCC	5 cut(s)	330, 417, 831, 1554, 1680
PspPI	GGNCC	5 cut(s)	241, 416, 829, 938, 1553
PspPPI	RGGWCCY	3 cut(s)	241, 829, 1553
PsrI	GAACNNNNNNTAC	2 cut(s)	1391, 1423
PstNI	CAGNNNCTG	1 cut(s)	349
PsuI	RGATCY	4 cut(s)	328, 388, 454, 730
RsaI	GTAC	4 cut(s)	57, 881, 1207, 1541
RsaNI	GTAC	4 cut(s)	56, 880, 1206, 1540
RseI	CAYNNNNRTG	4 cut(s)	978, 992, 1137, 1385
SaqAI	TTAA	2 cut(s)	282, 867
SatI	GCNGC	7 cut(s)	6, 202, 270, 477, 1014, 1620, 1623
Sau96I	GGNCC	5 cut(s)	241, 416, 829, 938, 1553
SchI	GAGTC	1 cut(s)	1596
ScrFI	CCNGG	8 cut(s)	170, 263, 344, 491, 705, 952, 1275, 1692
SfaNI	GCATC	3 cut(s)	1129, 1440, 1689
SgrAI	CRCCGGYG	1 cut(s)	1364
SinI	GGWCC	4 cut(s)	241, 829, 938, 1553
SmiMI	CAYNNNNRTG	4 cut(s)	978, 992, 1137, 1385
SmlI	CTYRAG	1 cut(s)	458
SmoI	CTYRAG	1 cut(s)	458
Sse9I	AATT	6 cut(s)	365, 632, 688, 1044, 1178, 1430
SseBI	AGGCCT	2 cut(s)	818, 851
SspMI	CTAG	3 cut(s)	9, 1557, 1638
StuI	AGGCCT	2 cut(s)	818, 851
StyD4I	CCNGG	8 cut(s)	168, 261, 342, 489, 703, 950, 1273, 1690
StyI	CCWWGG	6 cut(s)	129, 324, 594, 1038, 1421, 1556
TaaI	ACNGT	6 cut(s)	88, 647, 892, 938, 1210, 1594
TaiI	ACGT	2 cut(s)	771, 1074
TaqI	TCGA	7 cut(s)	156, 207, 302, 423, 621, 1053, 1460
TasI	AATT	6 cut(s)	365, 632, 688, 1044, 1178, 1430
TatI	WGTACW	1 cut(s)	55
TauI	GCSGC	4 cut(s)	272, 479, 1016, 1625
TfiI	GAWTC	2 cut(s)	14, 623
Tru1I	TTAA	2 cut(s)	282, 867
Tru9I	TTAA	2 cut(s)	282, 867
TscAI	CASTG	6 cut(s)	445, 705, 1006, 1098, 1207, 1678
TseFI	GTSAC	5 cut(s)	82, 221, 440, 932, 1297
TseI	GCWGC	3 cut(s)	5, 201, 1619
Tsp45I	GTSAC	5 cut(s)	82, 221, 440, 932, 1297
TspDTI	ATGAA	2 cut(s)	1010, 1207
TspGWI	ACGGA	1 cut(s)	1557
TspRI	CASTG	6 cut(s)	445, 705, 1006, 1098, 1207, 1678
Van91I	CCANNNNNTGG	2 cut(s)	349, 1087
VpaK11BI	GGWCC	4 cut(s)	241, 829, 938, 1553
XagI	CCTNNNNNAGG	2 cut(s)	209, 248
XapI	RAATTY	1 cut(s)	1430
XcmI	CCANNNNNNNNNTGG	4 cut(s)	331, 346, 705, 772
XmaJI	CCTAGG	1 cut(s)	1556
XmiI	GTMKAC	1 cut(s)	1212
XspI	CTAG	3 cut(s)	9, 1557, 1638
Zsp2I	ATGCAT	1 cut(s)	1144

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.