Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Forward (+)

13569818 .. 13572425

Gene Structure 2608 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1164200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1713 bp

ATGGCAGCTAGTCAAGCAAAGAAGAAGAAGATAGCCGTCATCATCGTATCGGTTGTACTTCTGGTGGCGATGCTAGTGGCGGTGATAGTGGGTGTCAAAATGGCCAAAAAAGGCGGCAACAAGAAGGCTCCAAAGGTTGGCAAGTCGAAAAAGGCCATCGAGACCATATGCCAACCCACAACCTACAAAGAAACCTGCCAGAGCAGCCTCGAGAAGGTGGCGGGTGACAGCACCGAGGCCAAGGACCTGATCAAGGCCGGGATCCAGGTGGCGGAAGACAAAGTTAACGAGGCGATCAAGAACTCGGACACCTTGAAAGAGCTTGCCAAGGATCCAATGGCCAGCCAGGCTTTGGACGTGTGCAAGCAACTGTTGGACACCGCAGTTGAGGATCTCCACGGTGCGGTGGATAAGATGGGGTCATTCGATCCTAACAAGACCGATGACTACCTTGAAGATCTCAAGGTGTGGCTGAGCGCCGCCGTCAATCACCAGGCAACCTGCCTTGACGCGTTTAAGGACACCAAGGGCGAGGCTGGTGAGAAAATGAAGGGCTTTTTGAAGACAGCGCAAGAACTCACCAGCAATGGACTTGCCATGGTCTCCGAAATCTCTGGTCTCGTCGGATCTCTTCACTTGAACTCTCACCGCCGCCTCCTGTCCTCGTCAAATGGCGGTCCGAAAAAGTGGCATGATAAGACAGTGCCAGGGTGGATGAGTGATCGCAAGCTTGATCTCTTGACTGCCACTCCGCAGAGCCTGAAGCCCGACGTGGTTGTGGCTCAAGATGGGACAGGCAAGTACAAGACCATCAACGAGGCCTTGAAGGAGGTCCCACCGAATAGCAAGAAGACCTTTGTGATTTATATTAAGGGGGGAGTGTACAAAGAGATTGTCGTCGTTAACAAGCAAATGACCAATGTTGTGATGATTGGTGACGGCCCAACCAAGACCAGGATCAGCGGAGGCTTGAACAAGGCGGATGGTGTCTCTACCGAGAACACCGCAACCTTTGCCGCACTTGGAAACTATTTCATTGCCAAGGGAATAGGGTTCGAGAACACAGCAGGATACATAAAGCACCAAGCTGTCGCATTGCGTGTGCAATCCGACTTCTCCATCTTCTACAACTGCCACATGGATGGATTCCAAGACACCCTCTACACCGAAGCCTACCGCCAATTCTACCGCGACTGCACCATCACTGGCACCATAGACTTCATCTTCGGCGATGGGGTGACTTTGTTCCAAAACTGCAAGATGATGGTGAGAAAACCAGCGGAAGGACAGTCTTGCATGGTGACAGCACAAGGCAGGACCGACCACAACGAGATCACCGGCATGTTCCTCCAAAACTGCACTATCACCGGTGAGCCGGACTATATGAATGTGAAGGAAAAGAGCAAGGCATATTTGGGGAGGCCATGGAAAGACTTTGCAAGGGTGATTGTGATGCAGTCATCCATTGACGAAGCCATTGTACCTGAAGGGTGGTCAGAATGGGTTGGTAAAAAAACCAACCAAACCTGCTTCTTTGGTGAGTACGGCAACCGGGGTCCTAGGGCTGACATGACCAAAAGGGTCAGATGGCCGACCGTCAAGGCGCTCACTTCTGATAAAGTCGCGGCTTTCACCCCTGCTAAGGTGTTTCTAGGTGTTGATTGGCTGAAGCCCAGTGGGGTTCCATATGTTCCTGGCATGATGTCCGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

571

Amino Acids

62.29

Weight (kDa)

9.01

Isoelectric Point (pI)

29.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	51 - 200	4.5e-31	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	257 - 549	2.3e-126	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	203, 509, 1535
AccB1I	GGYRCC	1 cut(s)	1208
AccB7I	CCANNNNNTGG	2 cut(s)	137, 352
AccII	CGCG	3 cut(s)	512, 1191, 1625
AclWI	GGATC	8 cut(s)	256, 269, 326, 339, 399, 422, 634, 965
AcoI	YGGCCR	3 cut(s)	102, 339, 1589
AcuI	CTGAAG	3 cut(s)	782, 1506, 1688
AfaI	GTAC	5 cut(s)	57, 803, 884, 1482, 1544
AfiI	CCNNNNNNNGG	9 cut(s)	137, 214, 253, 271, 352, 403, 954, 1283, 1642
AflIII	ACRYGT	2 cut(s)	357, 510
AgeI	ACCGGT	1 cut(s)	1367
AgsI	TTSAA	6 cut(s)	316, 455, 562, 640, 826, 973
AjiI	CACGTC	2 cut(s)	358, 772
AjnI	CCWGG	6 cut(s)	264, 345, 492, 706, 953, 1693
AluBI	AGCT	4 cut(s)	8, 322, 730, 1088
AluI	AGCT	4 cut(s)	8, 322, 730, 1088
Alw26I	GTCTC	4 cut(s)	155, 607, 623, 994
AlwI	GGATC	8 cut(s)	256, 269, 326, 339, 399, 422, 634, 965
AlwNI	CAGNNNCTG	1 cut(s)	760
Ama87I	CYCGRG	1 cut(s)	209
AoxI	GGCC	9 cut(s)	102, 153, 237, 255, 339, 819, 940, 1421, 1589
ApeKI	GCWGC	2 cut(s)	5, 204
ArsI	GACNNNNNNTTYG	4 cut(s)	879, 911, 1208, 1240
AsiGI	ACCGGT	1 cut(s)	1367
AspA2I	CCTAGG	1 cut(s)	1559
AspLEI	GCGC	3 cut(s)	479, 571, 1606
AspS9I	GGNCC	6 cut(s)	244, 677, 832, 941, 1317, 1556
AsuC2I	CCSGG	2 cut(s)	259, 1553
AvaI	CYCGRG	1 cut(s)	209
AvaII	GGWCC	5 cut(s)	244, 677, 832, 1317, 1556
AvrII	CCTAGG	1 cut(s)	1559
BalI	TGGCCA	2 cut(s)	104, 341
BamHI	GGATCC	2 cut(s)	261, 331
BanI	GGYRCC	1 cut(s)	1208
BarI	GAAGNNNNNNTAC	2 cut(s)	1464, 1496
BbsI	GAAGAC	3 cut(s)	282, 569, 857
BbvI	GCAGC	2 cut(s)	17, 216
BceAI	ACGGC	4 cut(s)	20, 467, 955, 1561
BciT130I	CCWGG	6 cut(s)	266, 347, 494, 708, 955, 1695
BciVI	GTATCC	1 cut(s)	1064
BclI	TGATCA	1 cut(s)	249
BcnI	CCSGG	2 cut(s)	259, 1553
BcoDI	GTCTC	4 cut(s)	155, 607, 623, 994
BfaI	CTAG	4 cut(s)	9, 74, 1560, 1652
BfoI	RGCGCY	2 cut(s)	480, 1607
BfuAI	ACCTGC	3 cut(s)	203, 509, 1535
BfuI	GTATCC	1 cut(s)	1064
BglI	GCCNNNNNGGC	1 cut(s)	347
BglII	AGATCT	1 cut(s)	457
BisI	GCNGC	7 cut(s)	6, 115, 205, 480, 652, 1017, 1626
BlnI	CCTAGG	1 cut(s)	1559
BlpI	GCTNAGC	1 cut(s)	473
BlsI	GCNGC	7 cut(s)	7, 116, 206, 481, 653, 1018, 1627
Bme1390I	CCNGG	8 cut(s)	259, 266, 347, 494, 708, 955, 1553, 1695
Bme18I	GGWCC	5 cut(s)	244, 677, 832, 1317, 1556
BmeT110I	CYCGRG	1 cut(s)	209
BmgBI	CACGTC	2 cut(s)	358, 772
BmgT120I	GGNCC	6 cut(s)	244, 677, 832, 941, 1317, 1556
BmiI	GGNNCC	7 cut(s)	129, 263, 333, 834, 1210, 1557, 1683
BmrFI	CCNGG	8 cut(s)	259, 266, 347, 494, 708, 955, 1553, 1695
BmrI	ACTGGG	1 cut(s)	1668
BmsI	GCATC	2 cut(s)	60, 1443
BmuI	ACTGGG	1 cut(s)	1668
BpiI	GAAGAC	3 cut(s)	282, 569, 857
Bpu10I	CCTNAGC	1 cut(s)	1641
Bpu1102I	GCTNAGC	1 cut(s)	473
BpuEI	CTTGAG	2 cut(s)	446, 768
BpuMI	CCSGG	2 cut(s)	259, 1553
BsaI	GGTCTC	3 cut(s)	155, 607, 623
BsaWI	WCCGGW	1 cut(s)	1367
BsaXI	ACNNNNNCTCC	2 cut(s)	733, 763
Bsc4I	CCNNNNNNNGG	9 cut(s)	137, 214, 253, 271, 352, 403, 954, 1283, 1642
Bse118I	RCCGGY	2 cut(s)	1337, 1367
Bse1I	ACTGG	2 cut(s)	1210, 1674
Bse3DI	GCAATG	3 cut(s)	592, 1035, 1094
BseBI	CCWGG	6 cut(s)	266, 347, 494, 708, 955, 1695
BseGI	GGATG	4 cut(s)	720, 988, 1147, 1460
BseLI	CCNNNNNNNGG	9 cut(s)	137, 214, 253, 271, 352, 403, 954, 1283, 1642
BseMI	GCAATG	3 cut(s)	592, 1035, 1094
BseMII	CTCAG	1 cut(s)	464
BseNI	ACTGG	2 cut(s)	1210, 1674
BseXI	GCAGC	2 cut(s)	17, 216
BsgI	GTGCAG	2 cut(s)	1180, 1342
Bsh1236I	CGCG	3 cut(s)	512, 1191, 1625
Bsh1285I	CGRYCG	1 cut(s)	1596
BshFI	GGCC	9 cut(s)	104, 155, 239, 257, 341, 821, 942, 1423, 1591
BshNI	GGYRCC	1 cut(s)	1208
BshTI	ACCGGT	1 cut(s)	1367
BsiEI	CGRYCG	1 cut(s)	1596
BsiHKCI	CYCGRG	1 cut(s)	209
BsiSI	CCGG	5 cut(s)	258, 1338, 1368, 1376, 1552
BslFI	GGGAC	2 cut(s)	805, 818
BslI	CCNNNNNNNGG	9 cut(s)	137, 214, 253, 271, 352, 403, 954, 1283, 1642
BsmAI	GTCTC	4 cut(s)	155, 607, 623, 994
BsmFI	GGGAC	2 cut(s)	805, 818
BsnI	GGCC	9 cut(s)	104, 155, 239, 257, 341, 821, 942, 1423, 1591
Bso31I	GGTCTC	3 cut(s)	155, 607, 623
BsoBI	CYCGRG	1 cut(s)	209
Bsp1407I	TGTACA	1 cut(s)	882
Bsp1720I	GCTNAGC	1 cut(s)	473
Bsp19I	CCATGG	2 cut(s)	597, 1424
BspANI	GGCC	9 cut(s)	104, 155, 239, 257, 341, 821, 942, 1423, 1591
BspCNI	CTCAG	1 cut(s)	465
BspFNI	CGCG	3 cut(s)	512, 1191, 1625
BspLI	GGNNCC	7 cut(s)	129, 263, 333, 834, 1210, 1557, 1683
BspMI	ACCTGC	3 cut(s)	203, 509, 1535
BspPI	GGATC	8 cut(s)	256, 269, 326, 339, 399, 422, 634, 965
BspT107I	GGYRCC	1 cut(s)	1208
BspTNI	GGTCTC	3 cut(s)	155, 607, 623
BsrDI	GCAATG	3 cut(s)	592, 1035, 1094
BsrFI	RCCGGY	2 cut(s)	1337, 1367
BsrGI	TGTACA	1 cut(s)	882
BsrI	ACTGG	2 cut(s)	1210, 1674
BssAI	RCCGGY	2 cut(s)	1337, 1367
BssT1I	CCWWGG	7 cut(s)	240, 327, 525, 597, 1041, 1424, 1559
Bst2UI	CCWGG	6 cut(s)	266, 347, 494, 708, 955, 1695
Bst4CI	ACNGT	5 cut(s)	372, 401, 703, 1290, 1597
Bst6I	CTCTTC	1 cut(s)	636
BstAPI	GCANNNNNTGC	1 cut(s)	1013
BstAUI	TGTACA	1 cut(s)	882
BstC8I	GCNNGC	4 cut(s)	324, 343, 365, 728
BstDEI	CTNAG	2 cut(s)	473, 1641
BstDSI	CCRYGG	3 cut(s)	397, 597, 1424
BstENI	CCTNNNNNAGG	2 cut(s)	212, 251
BstF5I	GGATG	4 cut(s)	720, 988, 1147, 1460
BstFNI	CGCG	3 cut(s)	512, 1191, 1625
BstH2I	RGCGCY	2 cut(s)	480, 1607
BstHHI	GCGC	3 cut(s)	479, 571, 1606
BstMAI	GTCTC	4 cut(s)	155, 607, 623, 994
BstMCI	CGRYCG	1 cut(s)	1596
BstMWI	GCNNNNNNNGC	4 cut(s)	14, 204, 347, 1013
BstNI	CCWGG	6 cut(s)	266, 347, 494, 708, 955, 1695
BstNSI	RCATGY	1 cut(s)	1345
BstSCI	CCNGG	8 cut(s)	257, 264, 345, 492, 706, 953, 1551, 1693
BstUI	CGCG	3 cut(s)	512, 1191, 1625
BstV1I	GCAGC	2 cut(s)	17, 216
BstV2I	GAAGAC	3 cut(s)	282, 569, 857
BstX2I	RGATCY	5 cut(s)	261, 331, 391, 457, 626
BstXI	CCANNNNNNTGG	1 cut(s)	1142
BstYI	RGATCY	5 cut(s)	261, 331, 391, 457, 626
BsuI	GTATCC	1 cut(s)	1064
BsuRI	GGCC	9 cut(s)	104, 155, 239, 257, 341, 821, 942, 1423, 1591
BtgI	CCRYGG	3 cut(s)	397, 597, 1424
BtgZI	GCGATG	2 cut(s)	83, 1245
BtrI	CACGTC	2 cut(s)	358, 772
BtsCI	GGATG	4 cut(s)	720, 988, 1147, 1460
BtsIMutI	CAGTG	3 cut(s)	708, 1203, 1681
BveI	ACCTGC	3 cut(s)	203, 509, 1535
Cac8I	GCNNGC	4 cut(s)	324, 343, 365, 728
CaiI	CAGNNNCTG	1 cut(s)	760
CfoI	GCGC	3 cut(s)	479, 571, 1606
Cfr10I	RCCGGY	2 cut(s)	1337, 1367
Cfr13I	GGNCC	6 cut(s)	244, 677, 832, 941, 1317, 1556
CpoI	CGGWCCG	1 cut(s)	677
CseI	GACGC	1 cut(s)	518
Csp6I	GTAC	5 cut(s)	56, 802, 883, 1481, 1543
CspAI	ACCGGT	1 cut(s)	1367
CspI	CGGWCCG	1 cut(s)	677
CviAII	CATG	8 cut(s)	598, 692, 1138, 1297, 1342, 1425, 1570, 1699
CviQI	GTAC	5 cut(s)	56, 802, 883, 1481, 1543
DdeI	CTNAG	2 cut(s)	473, 1641
EaeI	YGGCCR	3 cut(s)	102, 339, 1589
Eam1104I	CTCTTC	1 cut(s)	636
EarI	CTCTTC	1 cut(s)	636
EciI	GGCGGA	2 cut(s)	287, 995
Eco130I	CCWWGG	7 cut(s)	240, 327, 525, 597, 1041, 1424, 1559
Eco147I	AGGCCT	1 cut(s)	821
Eco31I	GGTCTC	3 cut(s)	155, 607, 623
Eco47I	GGWCC	5 cut(s)	244, 677, 832, 1317, 1556
Eco57I	CTGAAG	3 cut(s)	782, 1506, 1688
Eco88I	CYCGRG	1 cut(s)	209
EcoNI	CCTNNNNNAGG	2 cut(s)	212, 251
EcoO109I	RGGNCCY	3 cut(s)	244, 832, 1556
EcoRII	CCWGG	6 cut(s)	264, 345, 492, 706, 953, 1693
EcoT14I	CCWWGG	7 cut(s)	240, 327, 525, 597, 1041, 1424, 1559
ErhI	CCWWGG	7 cut(s)	240, 327, 525, 597, 1041, 1424, 1559
FaeI	CATG	8 cut(s)	601, 695, 1141, 1300, 1345, 1428, 1573, 1702
FalI	AAGNNNNNCTT	2 cut(s)	839, 871
FaqI	GGGAC	2 cut(s)	805, 818
FatI	CATG	8 cut(s)	597, 691, 1137, 1296, 1341, 1424, 1569, 1698
FauI	CCCGC	1 cut(s)	214
FauNDI	CATATG	2 cut(s)	167, 1687
FbaI	TGATCA	1 cut(s)	249
Fnu4HI	GCNGC	7 cut(s)	6, 115, 205, 480, 652, 1017, 1626
FokI	GGATG	4 cut(s)	727, 995, 1154, 1447
Fsp4HI	GCNGC	7 cut(s)	6, 115, 205, 480, 652, 1017, 1626
FspBI	CTAG	4 cut(s)	9, 74, 1560, 1652
GlaI	GCGC	3 cut(s)	478, 570, 1605
GluI	GCNGC	7 cut(s)	6, 115, 205, 480, 652, 1017, 1626
HaeII	RGCGCY	2 cut(s)	480, 1607
HaeIII	GGCC	9 cut(s)	104, 155, 239, 257, 341, 821, 942, 1423, 1591
HapII	CCGG	5 cut(s)	258, 1338, 1368, 1376, 1552
HgaI	GACGC	1 cut(s)	518
HhaI	GCGC	3 cut(s)	479, 571, 1606
Hin1II	CATG	8 cut(s)	601, 695, 1141, 1300, 1345, 1428, 1573, 1702
Hin6I	GCGC	3 cut(s)	477, 569, 1604
HinP1I	GCGC	3 cut(s)	477, 569, 1604
HincII	GTYRAC	2 cut(s)	286, 904
HindII	GTYRAC	2 cut(s)	286, 904
HindIII	AAGCTT	1 cut(s)	728
HinfI	GANTC	1 cut(s)	1146
HpaI	GTTAAC	2 cut(s)	286, 904
HpaII	CCGG	5 cut(s)	258, 1338, 1368, 1376, 1552
Hpy166II	GTNNAC	3 cut(s)	286, 883, 904
Hpy188I	TCNGA	8 cut(s)	307, 607, 626, 681, 1111, 1498, 1586, 1615
Hpy188III	TCNNGA	6 cut(s)	160, 211, 298, 739, 785, 1057
Hpy8I	GTNNAC	3 cut(s)	286, 883, 904
Hpy99I	CGWCG	3 cut(s)	626, 773, 902
HpyAV	CCTTC	7 cut(s)	118, 208, 544, 820, 1277, 1387, 1481
HpyCH4III	ACNGT	5 cut(s)	372, 401, 703, 1290, 1597
HpyCH4IV	ACGT	2 cut(s)	357, 771
HpyCH4V	TGCA	8 cut(s)	363, 1105, 1197, 1257, 1296, 1359, 1439, 1456
HpyF10VI	GCNNNNNNNGC	4 cut(s)	14, 204, 347, 1013
HpyF3I	CTNAG	2 cut(s)	473, 1641
HpySE526I	ACGT	2 cut(s)	357, 771
Hsp92II	CATG	8 cut(s)	601, 695, 1141, 1300, 1345, 1428, 1573, 1702
HspAI	GCGC	3 cut(s)	477, 569, 1604
Ksp22I	TGATCA	1 cut(s)	249
KspAI	GTTAAC	2 cut(s)	286, 904
LmnI	GCTCC	1 cut(s)	133
Lsp1109I	GCAGC	2 cut(s)	17, 216
LweI	GCATC	2 cut(s)	60, 1443
MaeI	CTAG	4 cut(s)	9, 74, 1560, 1652
MaeII	ACGT	2 cut(s)	357, 771
MaeIII	GTNAC	4 cut(s)	224, 935, 1237, 1300
MflI	RGATCY	5 cut(s)	261, 331, 391, 457, 626
MlsI	TGGCCA	2 cut(s)	104, 341
MluCI	AATT	1 cut(s)	1181
MluI	ACGCGT	1 cut(s)	510
MluNI	TGGCCA	2 cut(s)	104, 341
MmeI	TCCRAC	3 cut(s)	354, 604, 1134
Mox20I	TGGCCA	2 cut(s)	104, 341
MscI	TGGCCA	2 cut(s)	104, 341
MseI	TTAA	4 cut(s)	285, 516, 870, 903
MslI	CAYNNNNRTG	2 cut(s)	1140, 1340
Msp20I	TGGCCA	2 cut(s)	104, 341
MspA1I	CMGCKG	2 cut(s)	963, 1280
MspI	CCGG	5 cut(s)	258, 1338, 1368, 1376, 1552
MspR9I	CCNGG	8 cut(s)	259, 266, 347, 494, 708, 955, 1553, 1695
MvaI	CCWGG	6 cut(s)	266, 347, 494, 708, 955, 1695
MvnI	CGCG	3 cut(s)	512, 1191, 1625
MwoI	GCNNNNNNNGC	4 cut(s)	14, 204, 347, 1013
NciI	CCSGG	2 cut(s)	259, 1553
NcoI	CCATGG	2 cut(s)	597, 1424
NdeI	CATATG	2 cut(s)	167, 1687
NlaIII	CATG	8 cut(s)	601, 695, 1141, 1300, 1345, 1428, 1573, 1702
NlaIV	GGNNCC	7 cut(s)	129, 263, 333, 834, 1210, 1557, 1683
NmuCI	GTSAC	4 cut(s)	224, 935, 1237, 1300
NspI	RCATGY	1 cut(s)	1345
PaeR7I	CTCGAG	1 cut(s)	209
PceI	AGGCCT	1 cut(s)	821
PfeI	GAWTC	1 cut(s)	1146
PflMI	CCANNNNNTGG	2 cut(s)	137, 352
PinAI	ACCGGT	1 cut(s)	1367
PkrI	GCNGC	7 cut(s)	7, 116, 206, 481, 653, 1018, 1627
PpuMI	RGGWCCY	3 cut(s)	244, 832, 1556
Psp5II	RGGWCCY	3 cut(s)	244, 832, 1556
Psp6I	CCWGG	6 cut(s)	264, 345, 492, 706, 953, 1693
PspGI	CCWGG	6 cut(s)	264, 345, 492, 706, 953, 1693
PspN4I	GGNNCC	7 cut(s)	129, 263, 333, 834, 1210, 1557, 1683
PspPI	GGNCC	6 cut(s)	244, 677, 832, 941, 1317, 1556
PspPPI	RGGWCCY	3 cut(s)	244, 832, 1556
PstNI	CAGNNNCTG	1 cut(s)	760
PsuI	RGATCY	5 cut(s)	261, 331, 391, 457, 626
RsaI	GTAC	5 cut(s)	57, 803, 884, 1482, 1544
RsaNI	GTAC	5 cut(s)	56, 802, 883, 1481, 1543
RseI	CAYNNNNRTG	2 cut(s)	1140, 1340
Rsr2I	CGGWCCG	1 cut(s)	677
RsrII	CGGWCCG	1 cut(s)	677
SaqAI	TTAA	4 cut(s)	285, 516, 870, 903
SatI	GCNGC	7 cut(s)	6, 115, 205, 480, 652, 1017, 1626
Sau96I	GGNCC	6 cut(s)	244, 677, 832, 941, 1317, 1556
ScrFI	CCNGG	8 cut(s)	259, 266, 347, 494, 708, 955, 1553, 1695
SfaNI	GCATC	2 cut(s)	60, 1443
Sfr274I	CTCGAG	1 cut(s)	209
SgrAI	CRCCGGYG	1 cut(s)	1367
SinI	GGWCC	5 cut(s)	244, 677, 832, 1317, 1556
SlaI	CTCGAG	1 cut(s)	209
SmiMI	CAYNNNNRTG	2 cut(s)	1140, 1340
SmlI	CTYRAG	3 cut(s)	209, 461, 783
SmoI	CTYRAG	3 cut(s)	209, 461, 783
Sse9I	AATT	1 cut(s)	1181
SseBI	AGGCCT	1 cut(s)	821
SspMI	CTAG	4 cut(s)	9, 74, 1560, 1652
StuI	AGGCCT	1 cut(s)	821
StyD4I	CCNGG	8 cut(s)	257, 264, 345, 492, 706, 953, 1551, 1693
StyI	CCWWGG	7 cut(s)	240, 327, 525, 597, 1041, 1424, 1559
TaaI	ACNGT	5 cut(s)	372, 401, 703, 1290, 1597
TaiI	ACGT	2 cut(s)	360, 774
TaqI	TCGA	5 cut(s)	146, 159, 210, 426, 1056
TaqII	GACCGA	2 cut(s)	455, 1334
TasI	AATT	1 cut(s)	1181
TatI	WGTACW	3 cut(s)	55, 801, 882
TauI	GCSGC	5 cut(s)	117, 482, 654, 1019, 1628
TfiI	GAWTC	1 cut(s)	1146
Tru1I	TTAA	4 cut(s)	285, 516, 870, 903
Tru9I	TTAA	4 cut(s)	285, 516, 870, 903
TscAI	CASTG	3 cut(s)	708, 1210, 1681
TseFI	GTSAC	4 cut(s)	224, 935, 1237, 1300
TseI	GCWGC	2 cut(s)	5, 204
Tsp45I	GTSAC	4 cut(s)	224, 935, 1237, 1300
TspDTI	ATGAA	4 cut(s)	563, 1024, 1210, 1400
TspGWI	ACGGA	1 cut(s)	1696
TspRI	CASTG	3 cut(s)	708, 1210, 1681
Van91I	CCANNNNNTGG	2 cut(s)	137, 352
VpaK11BI	GGWCC	5 cut(s)	244, 677, 832, 1317, 1556
XagI	CCTNNNNNAGG	2 cut(s)	212, 251
XceI	RCATGY	1 cut(s)	1345
XcmI	CCANNNNNNNNNTGG	2 cut(s)	334, 349
XhoI	CTCGAG	1 cut(s)	209
XmaJI	CCTAGG	1 cut(s)	1559
XspI	CTAG	4 cut(s)	9, 74, 1560, 1652

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.