RchiOBHm_Chr4g0423371

Acts in the modification of cell walls via demethylesterification of cell wall pectin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000809
Physical Location & Seq
Reverse (-)
48966336 .. 48968565
2230 bp
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UTR
Exon/CDS
Intron
PRQ39275

Sequence Viewer

Length: 1707 bp
ATGAGCGGAGATGACCGTCACGCCAAGAAGAAGAAGTTCGCCATCCTCGGCGTGTCGGCGTTGATTCTGGTGGCCATGGTGGTTGCTGTGGCTGTTGGGGTCACCAAAAGCCAGAAAGGCGGAAAGGACGAGAAGAAACCAGCCACAACATCCAAGGCAATTGAAGCCGTCTGCCAACCAACAGACTACAAGGAAACTTGCCAAAACAGCCTCTCCTCAAAAGCCAGCAACGTCACTGACCCAAAAGAGCTCATCAAGATTGGGTTCCAGATCGCCATAGACAAAATCCAAGAGGTGATCAACAACTCCCCCACCTTGAAAGAGCTTGCCAAGGATCCGAGGAGCAACCAGGCCCTTCAGAACTGCAAAGAACTCTTAGAGTTCTCCATCAATGATCTGAAGAAGTCGGTCGAGCATATCGGCCCCTTCGAAGTTAGCAAGCTCAAAGCATATGTCGACGACCTTAAGGTTTGGCTTAGTGCTTCCATCAGCTACGAACAGACTTGCTTGGATGGATTCGAGGACACCAAGGGTGAGGCTGGGACCAAAATGCAGGGCTTCTTAAAGCAATCACAAGAACTCACCAAGAACGGACTTGCCATGGTCACTGAAATAGGCAACCTCTTGGTAAATGGCAACCTTAAAACCCAACGTCGTCTCTTAGCTGAGGCTGAGGCTCCTATAGACCAAAAGAAGGCAAATAAGATCATCCCCTCATGGATTGATGGACGCAAGCTCGACCTCGCTACCGCCAATCCCAGCACCCTTAAACCCGACGTTGTAGTCGCTGCGGATGGAAGTGAAAATTACAAGACCATCAACGAGGCTCTCCAGGATATGCCCAAGAACGGTGGTGAAAAAACGTTTGTGATCTACATCAAAGAGGGAACGTATGATGAGAATGTGTTGGTTGACAAGCATATGACCAATGTCATGTTCATCGGTGATGGCCCTACCAAGACCAAGATCACGGGTAACAAGAACTATGCCGATGGTGTCCAAACATACAAAACCGCAACTGTCGCTGTGATCGGAGACTACTTCATTGCTAAGGACATAGGATTCGAGAACACAGCCGGACCTAAAGGACACCAAGCCGTGGCACTGCGTGTTCAATCTGATCTGTCCATCTTCTACAACTGCCAGATAGACGGTTACCAAAACACACTCTTCACCCAAACACACAGGCAATTCTACCGAGACTGCACCATCTCTGGAACCATCGACTTCATCTTCGGTGATGCAGTCGCCGTGTTCCAAAACTGCAAGTTGATCATCAAGAAGCCCGACTCAGATCAGAGCTGCTTTGTCACCTCCCAAGGAAGGAGTGACAAGCGGCAGCCCACTGCCATCGCCCTCCAAAATTGCACCATTAGCGGCGAGAAGGACTATGTGAACAACAAAGGTGCCTACTTGGGCAGGCCACAACACGCCTACTCTAGGACCATCGTGATGCAGTCCGAGATCGATGACGTTATCAGCCCTGAGGGTTGGATGGAGTGGACTGGTAAGACCAACCACGAAACATGCTGGTTCGGCGAGTTCTCAAACAGGGGACGTGGTGCGGCCACCAACAAGAGGGTGAATTGGTACAAGTTGGTCACCCGTGAACAAGCCGCCGAATTCACCCCCGGCGGAATTCTTTCTGGTGATAGGTGGATCAAGCCCAGCGGTGTGCCGTATGAGGCTGGAATGATGCGTGTCTAA

Protein Analysis

568

Amino Acids

62.67

Weight (kDa)

8.3

Isoelectric Point (pI)

29.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 53 - 201 5.1e-32 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 259 - 551 3e-123 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 782
AccB1I GGYRCC 1 cut(s) 1406
AccB7I CCANNNNNTGG 1 cut(s) 1099
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 456
AclI AACGTT 1 cut(s) 863
AclWI GGATC 3 cut(s) 329, 342, 1667
AcoI YGGCCR 2 cut(s) 72, 1566
AcsI RAATTY 2 cut(s) 1622, 1638
AcuI CTGAAG 2 cut(s) 341, 419
AdeI CACNNNGTG 1 cut(s) 1109
AfaI GTAC 1 cut(s) 1592
AfiI CCNNNNNNNGG 6 cut(s) 694, 848, 1099, 1323, 1440, 1578
AflII CTTAAG 1 cut(s) 464
AgsI TTSAA 3 cut(s) 164, 319, 1115
AjiI CACGTC 1 cut(s) 1559
AjnI CCWGG 2 cut(s) 348, 831
AjuI GAANNNNNNNTTGG 2 cut(s) 920, 952
AluBI AGCT 7 cut(s) 250, 325, 442, 492, 665, 736, 1302
AluI AGCT 7 cut(s) 250, 325, 442, 492, 665, 736, 1302
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 3 cut(s) 662, 1029, 1194
AlwI GGATC 3 cut(s) 329, 342, 1667
AoxI GGCC 6 cut(s) 72, 351, 421, 949, 1421, 1566
ApeKI GCWGC 3 cut(s) 788, 1302, 1339
ApoI RAATTY 2 cut(s) 1622, 1638
ArsI GACNNNNNNTTYG 6 cut(s) 438, 470, 1046, 1078, 1217, 1249
Asp700I GAANNNNTTC 2 cut(s) 35, 1642
AspS9I GGNCC 6 cut(s) 352, 422, 543, 950, 1079, 1443
AsuC2I CCSGG 1 cut(s) 1632
AsuII TTCGAA 1 cut(s) 429
AvaII GGWCC 3 cut(s) 543, 1079, 1443
AxyI CCTNAGG 1 cut(s) 1485
BalI TGGCCA 1 cut(s) 74
BamHI GGATCC 1 cut(s) 334
BanI GGYRCC 1 cut(s) 1406
BanII GRGCYC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 252
BbvCI CCTCAGC 2 cut(s) 666, 672
BbvI GCAGC 3 cut(s) 775, 1289, 1351
BceAI ACGGC 4 cut(s) 152, 1082, 1235, 1663
BciT130I CCWGG 2 cut(s) 350, 833
BclI TGATCA 2 cut(s) 297, 1272
BcnI CCSGG 1 cut(s) 1632
BcoDI GTCTC 3 cut(s) 662, 1029, 1194
BfaI CTAG 1 cut(s) 1440
BfmI CTRYAG 1 cut(s) 681
BfrI CTTAAG 1 cut(s) 464
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 7 cut(s) 789, 1303, 1337, 1340, 1378, 1566, 1617
BlsI GCNGC 7 cut(s) 790, 1304, 1338, 1341, 1379, 1567, 1618
Bme1390I CCNGG 3 cut(s) 350, 833, 1632
Bme18I GGWCC 3 cut(s) 543, 1079, 1443
BmgBI CACGTC 1 cut(s) 1559
BmgT120I GGNCC 6 cut(s) 352, 422, 543, 950, 1079, 1443
BmiI GGNNCC 7 cut(s) 266, 336, 424, 544, 678, 1219, 1408
BmrFI CCNGG 3 cut(s) 350, 833, 1632
BmsI GCATC 3 cut(s) 1231, 1443, 1686
BoxI GACNNNNGTC 1 cut(s) 929
BpmI CTGGAG 1 cut(s) 815
Bpu10I CCTNAGC 3 cut(s) 666, 672, 1050
Bpu14I TTCGAA 1 cut(s) 429
BpuMI CCSGG 1 cut(s) 1632
Bsa29I ATCGAT 1 cut(s) 1467
BsaBI GATNNNNATC 1 cut(s) 875
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bsc4I CCNNNNNNNGG 6 cut(s) 694, 848, 1099, 1323, 1440, 1578
Bse1I ACTGG 1 cut(s) 1510
Bse21I CCTNAGG 1 cut(s) 1485
Bse3DI GCAATG 1 cut(s) 1044
Bse8I GATNNNNATC 1 cut(s) 875
BseBI CCWGG 2 cut(s) 350, 833
BseCI ATCGAT 1 cut(s) 1467
BseGI GGATG 6 cut(s) 42, 149, 517, 708, 799, 1500
BseJI GATNNNNATC 1 cut(s) 875
BseLI CCNNNNNNNGG 6 cut(s) 694, 848, 1099, 1323, 1440, 1578
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 4 cut(s) 657, 663, 1305, 1476
BseNI ACTGG 1 cut(s) 1510
BseRI GAGGAG 2 cut(s) 205, 355
BseXI GCAGC 3 cut(s) 775, 1289, 1351
BseYI CCCAGC 3 cut(s) 539, 758, 1667
BsgI GTGCAG 1 cut(s) 1189
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 6 cut(s) 74, 353, 423, 951, 1423, 1568
BshNI GGYRCC 1 cut(s) 1406
BshVI ATCGAT 1 cut(s) 1467
BsiEI CGRYCG 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 252
BsiSI CCGG 2 cut(s) 1077, 1632
BslFI GGGAC 2 cut(s) 556, 1569
BslI CCNNNNNNNGG 6 cut(s) 694, 848, 1099, 1323, 1440, 1578
BsmAI GTCTC 3 cut(s) 662, 1029, 1194
BsmBI CGTCTC 1 cut(s) 662
BsmFI GGGAC 2 cut(s) 556, 1569
BsnI GGCC 6 cut(s) 74, 353, 423, 951, 1423, 1568
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 252
Bsp19I CCATGG 2 cut(s) 75, 600
BspANI GGCC 6 cut(s) 74, 353, 423, 951, 1423, 1568
BspCNI CTCAG 4 cut(s) 658, 664, 1304, 1477
BspDI ATCGAT 1 cut(s) 1467
BspLI GGNNCC 7 cut(s) 266, 336, 424, 544, 678, 1219, 1408
BspPI GGATC 3 cut(s) 329, 342, 1667
BspT104I TTCGAA 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 1406
BspTI CTTAAG 1 cut(s) 464
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 1044
BsrI ACTGG 1 cut(s) 1510
BssT1I CCWWGG 6 cut(s) 75, 153, 330, 528, 600, 1318
Bst2UI CCWGG 2 cut(s) 350, 833
Bst4CI ACNGT 4 cut(s) 17, 851, 1021, 1154
Bst6I CTCTTC 1 cut(s) 1175
BstAFI CTTAAG 1 cut(s) 464
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 5 cut(s) 226, 327, 440, 734, 1421
BstDEI CTNAG 8 cut(s) 376, 476, 661, 666, 672, 1050, 1291, 1485
BstDSI CCRYGG 3 cut(s) 75, 600, 1098
BstEII GGTNACC 3 cut(s) 100, 1154, 1600
BstENI CCTNNNNNAGG 1 cut(s) 1438
BstF5I GGATG 6 cut(s) 42, 149, 517, 708, 799, 1500
BstMAI GTCTC 3 cut(s) 662, 1029, 1194
BstMCI CGRYCG 1 cut(s) 411
BstMWI GCNNNNNNNGC 6 cut(s) 117, 164, 207, 1022, 1374, 1536
BstNI CCWGG 2 cut(s) 350, 833
BstNSI RCATGY 1 cut(s) 1530
BstPAI GACNNNNGTC 1 cut(s) 929
BstPI GGTNACC 3 cut(s) 100, 1154, 1600
BstSCI CCNGG 3 cut(s) 348, 831, 1630
BstSFI CTRYAG 1 cut(s) 681
BstV1I GCAGC 3 cut(s) 775, 1289, 1351
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
Bsu15I ATCGAT 1 cut(s) 1467
Bsu36I CCTNAGG 1 cut(s) 1485
BsuRI GGCC 6 cut(s) 74, 353, 423, 951, 1423, 1568
BsuTUI ATCGAT 1 cut(s) 1467
BtgI CCRYGG 3 cut(s) 75, 600, 1098
BtgZI GCGATG 1 cut(s) 1336
BtrI CACGTC 1 cut(s) 1559
BtsCI GGATG 6 cut(s) 42, 149, 517, 708, 799, 1500
BtsI GCAGTG 2 cut(s) 1103, 1344
BtsIMutI CAGTG 4 cut(s) 234, 606, 1103, 1344
Cac8I GCNNGC 5 cut(s) 226, 327, 440, 734, 1421
Cfr13I GGNCC 6 cut(s) 352, 422, 543, 950, 1079, 1443
ClaI ATCGAT 1 cut(s) 1467
CseI GACGC 1 cut(s) 738
Csp6I GTAC 1 cut(s) 1591
CspCI CAANNNNNGTGG 2 cut(s) 832, 867
CviAII CATG 5 cut(s) 76, 601, 717, 934, 1527
CviQI GTAC 1 cut(s) 1591
DdeI CTNAG 8 cut(s) 376, 476, 661, 666, 672, 1050, 1291, 1485
DraIII CACNNNGTG 1 cut(s) 1109
DrdI GACNNNNNNGTC 1 cut(s) 782
DseDI GACNNNNNNGTC 1 cut(s) 782
EaeI YGGCCR 2 cut(s) 72, 1566
Eam1104I CTCTTC 1 cut(s) 1175
EarI CTCTTC 1 cut(s) 1175
EciI GGCGGA 2 cut(s) 135, 1650
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 6 cut(s) 75, 153, 330, 528, 600, 1318
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 3 cut(s) 543, 1079, 1443
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 2 cut(s) 341, 419
Eco81I CCTNAGG 1 cut(s) 1485
Eco91I GGTNACC 3 cut(s) 100, 1154, 1600
EcoICRI GAGCTC 1 cut(s) 250
EcoNI CCTNNNNNAGG 1 cut(s) 1438
EcoO109I RGGNCCY 1 cut(s) 352
EcoO65I GGTNACC 3 cut(s) 100, 1154, 1600
EcoRI GAATTC 2 cut(s) 1622, 1638
EcoRII CCWGG 2 cut(s) 348, 831
EcoT14I CCWWGG 6 cut(s) 75, 153, 330, 528, 600, 1318
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 6 cut(s) 75, 153, 330, 528, 600, 1318
Esp3I CGTCTC 1 cut(s) 662
FaeI CATG 5 cut(s) 79, 604, 720, 937, 1530
FaqI GGGAC 2 cut(s) 556, 1569
FatI CATG 5 cut(s) 75, 600, 716, 933, 1526
FauNDI CATATG 2 cut(s) 451, 921
FbaI TGATCA 2 cut(s) 297, 1272
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 7 cut(s) 789, 1303, 1337, 1340, 1378, 1566, 1617
FokI GGATG 6 cut(s) 29, 136, 524, 695, 806, 1507
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 7 cut(s) 789, 1303, 1337, 1340, 1378, 1566, 1617
FspBI CTAG 1 cut(s) 1440
GluI GCNGC 7 cut(s) 789, 1303, 1337, 1340, 1378, 1566, 1617
GsaI CCCAGC 3 cut(s) 543, 762, 1671
GsuI CTGGAG 1 cut(s) 815
HaeIII GGCC 6 cut(s) 74, 353, 423, 951, 1423, 1568
HapII CCGG 2 cut(s) 1077, 1632
HgaI GACGC 1 cut(s) 738
Hin1II CATG 5 cut(s) 79, 604, 720, 937, 1530
HincII GTYRAC 2 cut(s) 457, 913
HindII GTYRAC 2 cut(s) 457, 913
HinfI GANTC 4 cut(s) 64, 516, 1062, 1289
HpaII CCGG 2 cut(s) 1077, 1632
Hpy166II GTNNAC 5 cut(s) 457, 913, 1396, 1503, 1610
Hpy188I TCNGA 8 cut(s) 339, 360, 399, 1034, 1120, 1294, 1299, 1462
Hpy188III TCNNGA 6 cut(s) 256, 268, 1066, 1215, 1279, 1450
Hpy8I GTNNAC 5 cut(s) 457, 913, 1396, 1503, 1610
Hpy99I CGWCG 3 cut(s) 461, 657, 779
HpyAV CCTTC 5 cut(s) 365, 436, 688, 1317, 1378
HpyCH4III ACNGT 4 cut(s) 17, 851, 1021, 1154
HpyCH4IV ACGT 7 cut(s) 231, 652, 777, 863, 890, 1473, 1558
HpyCH4V TGCA 7 cut(s) 366, 553, 1206, 1244, 1266, 1368, 1456
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 164, 207, 1022, 1374, 1536
HpyF3I CTNAG 8 cut(s) 376, 476, 661, 666, 672, 1050, 1291, 1485
HpySE526I ACGT 7 cut(s) 231, 652, 777, 863, 890, 1473, 1558
Hsp92II CATG 5 cut(s) 79, 604, 720, 937, 1530
Ksp22I TGATCA 2 cut(s) 297, 1272
LmnI GCTCC 2 cut(s) 342, 682
Lsp1109I GCAGC 3 cut(s) 775, 1289, 1351
LweI GCATC 3 cut(s) 1231, 1443, 1686
MaeI CTAG 1 cut(s) 1440
MaeII ACGT 7 cut(s) 231, 652, 777, 863, 890, 1473, 1558
MaeIII GTNAC 9 cut(s) 17, 100, 232, 604, 974, 1154, 1309, 1328, 1600
MbiI CCGCTC 1 cut(s) 6
MboII GAAGA 7 cut(s) 40, 43, 145, 412, 1123, 1162, 1225
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 252
MlsI TGGCCA 1 cut(s) 74
MluCI AATT 7 cut(s) 159, 805, 1190, 1363, 1585, 1622, 1638
MluNI TGGCCA 1 cut(s) 74
MlyI GAGTC 1 cut(s) 1283
MmeI TCCRAC 1 cut(s) 1472
Mox20I TGGCCA 1 cut(s) 74
MroXI GAANNNNTTC 2 cut(s) 35, 1642
MscI TGGCCA 1 cut(s) 74
MseI TTAA 4 cut(s) 465, 563, 642, 768
MslI CAYNNNNRTG 1 cut(s) 1451
Msp20I TGGCCA 1 cut(s) 74
MspA1I CMGCKG 1 cut(s) 1671
MspCI CTTAAG 1 cut(s) 464
MspI CCGG 2 cut(s) 1077, 1632
MspR9I CCNGG 3 cut(s) 350, 833, 1632
MunI CAATTG 1 cut(s) 159
MvaI CCWGG 2 cut(s) 350, 833
MwoI GCNNNNNNNGC 6 cut(s) 117, 164, 207, 1022, 1374, 1536
NciI CCSGG 1 cut(s) 1632
NcoI CCATGG 2 cut(s) 75, 600
NdeI CATATG 2 cut(s) 451, 921
NlaIII CATG 5 cut(s) 79, 604, 720, 937, 1530
NlaIV GGNNCC 7 cut(s) 266, 336, 424, 544, 678, 1219, 1408
NmeAIII GCCGAG 1 cut(s) 27
NmuCI GTSAC 7 cut(s) 17, 100, 232, 604, 1309, 1328, 1600
NspI RCATGY 1 cut(s) 1530
NspV TTCGAA 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 426
PdmI GAANNNNTTC 2 cut(s) 35, 1642
PfeI GAWTC 3 cut(s) 64, 516, 1062
PflMI CCANNNNNTGG 1 cut(s) 1099
PfoI TCCNGGA 1 cut(s) 831
PkrI GCNGC 7 cut(s) 790, 1304, 1338, 1341, 1379, 1567, 1618
PleI GAGTC 1 cut(s) 1283
PpsI GAGTC 1 cut(s) 1283
PshAI GACNNNNGTC 1 cut(s) 929
Psp124BI GAGCTC 1 cut(s) 252
Psp1406I AACGTT 1 cut(s) 863
Psp6I CCWGG 2 cut(s) 348, 831
PspEI GGTNACC 3 cut(s) 100, 1154, 1600
PspFI CCCAGC 3 cut(s) 539, 758, 1667
PspGI CCWGG 2 cut(s) 348, 831
PspN4I GGNNCC 7 cut(s) 266, 336, 424, 544, 678, 1219, 1408
PspPI GGNCC 6 cut(s) 352, 422, 543, 950, 1079, 1443
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 1 cut(s) 1592
RsaNI GTAC 1 cut(s) 1591
RseI CAYNNNNRTG 1 cut(s) 1451
SacI GAGCTC 1 cut(s) 252
SalI GTCGAC 1 cut(s) 455
SaqAI TTAA 4 cut(s) 465, 563, 642, 768
SatI GCNGC 7 cut(s) 789, 1303, 1337, 1340, 1378, 1566, 1617
Sau96I GGNCC 6 cut(s) 352, 422, 543, 950, 1079, 1443
SchI GAGTC 1 cut(s) 1283
ScrFI CCNGG 3 cut(s) 350, 833, 1632
SduI GDGCHC 1 cut(s) 252
SfaNI GCATC 3 cut(s) 1231, 1443, 1686
SfcI CTRYAG 1 cut(s) 681
SfuI TTCGAA 1 cut(s) 429
SinI GGWCC 3 cut(s) 543, 1079, 1443
SmiMI CAYNNNNRTG 1 cut(s) 1451
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 7 cut(s) 159, 805, 1190, 1363, 1585, 1622, 1638
SspMI CTAG 1 cut(s) 1440
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 3 cut(s) 348, 831, 1630
StyI CCWWGG 6 cut(s) 75, 153, 330, 528, 600, 1318
TaaI ACNGT 4 cut(s) 17, 851, 1021, 1154
TaiI ACGT 7 cut(s) 234, 655, 780, 866, 893, 1476, 1561
TaqI TCGA 8 cut(s) 411, 429, 456, 519, 738, 1065, 1224, 1467
TaqII GACCGA 1 cut(s) 397
TasI AATT 7 cut(s) 159, 805, 1190, 1363, 1585, 1622, 1638
TauI GCSGC 4 cut(s) 1339, 1380, 1568, 1619
TfiI GAWTC 3 cut(s) 64, 516, 1062
Tru1I TTAA 4 cut(s) 465, 563, 642, 768
Tru9I TTAA 4 cut(s) 465, 563, 642, 768
TscAI CASTG 4 cut(s) 241, 613, 1110, 1351
TseFI GTSAC 7 cut(s) 17, 100, 232, 604, 1309, 1328, 1600
TseI GCWGC 3 cut(s) 788, 1302, 1339
Tsp45I GTSAC 7 cut(s) 17, 100, 232, 604, 1309, 1328, 1600
TspDTI ATGAA 3 cut(s) 928, 1033, 1219
TspGWI ACGGA 1 cut(s) 606
TspRI CASTG 4 cut(s) 241, 613, 1110, 1351
Van91I CCANNNNNTGG 1 cut(s) 1099
Vha464I CTTAAG 1 cut(s) 464
VpaK11BI GGWCC 3 cut(s) 543, 1079, 1443
XagI CCTNNNNNAGG 1 cut(s) 1438
XapI RAATTY 2 cut(s) 1622, 1638
XceI RCATGY 1 cut(s) 1530
XmiI GTMKAC 1 cut(s) 456
XmnI GAANNNNTTC 2 cut(s) 35, 1642
XspI CTAG 1 cut(s) 1440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.