Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Reverse (-)

47151960 .. 47154382

Gene Structure 2423 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00365590.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGGGCGTCCCTGGTGGTAATAAGAGGATCATAATCATTACGGTCTCTTCGGCGCTTCTGGTGGCCATGGTGGTCGGTGCGGTGGTTGGTGTGATCCAATACAAAAAACAGGAGCAACGCGGAGGCGGAGGCGGAGGAGGAGGCGGAGGGGGAGACGGAGACGGAGACGGAGACTCTAAACCCGGAGATAAAAACGCCGTCCGTACATCAAAGCCAATTGTAGCCATTTGCCAGCCCACCAACTACAAACAAGAATGTGAGAATAGTCTGCAAAAGGAGGCCGGAGACGAAAGAGACCCGAAAGACCTCGCAAGAGTGGGGATACAGGTGGCCATTAACAAGCTGGATGAGGCTATAAGGAACTCCAAAACCTTGCCAGATCAGGCCAAAGATCCCATGACCAAACAAGCTCTACATATCTGCAACCAGCTGTTGAATGCTTCTATGTACGATTTGAAAATTTCTTTCAATAAAATGGGTGCATTAGATCTCGAAAAGGTTGATGAATACGCGAAAGATGTTGAGGTTTGGCTGAGTGGGGCTGTCAGTCACCAAGCGACTTGCATTGATGAATCTCAGAACACAACCGAAGATGTCGGCAAGAAAATGAAGGAAATCTTGAAGTCAGCTCATGAAAGCACTAGCAATGCACTTGCCATGGTCACCGAAATCACTAACGCTTTGAACTCTCTTCATTTTAAATCGAACCGACGCCGTCTCCTGGCCTCATCGCCGCCATCCAAGAATAACAACAATAATAAGAATAATCAACACAAGCAGAATGGCAACAAAAGTAATCAGGGTCTCCCTTCTTGGCTCACTGATCGTAAGTTGGATCTTCTGACAGCTACGCCTGAAACCCTTAAGCCCAACGTGACGGTGGCTCAAGATGGGAACAGTAAGTATAAAACCATCAACGAAGCTTTGAAACTGGTCCCAAAGAAGAGCAACGACTTGTTTGTGATTTACGTGAAGGAGGGAGTGTACAAAGAAAGTGTGACGGTTGCAGGTATGATGGAAAATGTTGTGATGATTGGTGATGGCCCTACTAAGTCCAGGATCTCCGGTGACAATAACTTTGCTAGCGGAACCAGAACTATGTACACTGCAACAGTTGCTGTAGTTGGAAATTTTTTTGTCGCCAAGGACATAGGGTTTGAGAACACAGCCGGAGCGAAAGGGCACCAAGCGGTGGCACTGCGTGTTCAATCGGACTTCTCCATATTCTACAACTGCCACATTGACGGGTACCAAAACACCCTATACGTCCAAGCCTACAGGAATTACTTCCGCGACTGCAACATCACTGGCACCATTGACTTCATCTTCGGTGACGCTGTCTCGCTCTTCCAGAACTGCGAGATTGTGGTGAGGAAGCCAATGGACAACCAGACCTGCATCGTCACTGCCCAAGGAAAGACAGACAAGAATGAGGTCACCGGCATCGTGCTCCAAAACTGCACCATCAGAGGCGACCCAGAGTACGAGAACAAGGGATACCTAGGTCGGCCATGGAAGAATTTCGCAAAAACTATCGTCATGCAGTCACAGATTGATGATGTGATTCAACCTGAAGGGTACATGCCTTGGGACGGCGCCAAGTTCCACATGACGTCATTTTTCGGGGAGTACGACAACAGGGGACCTGGCGCTGACATGTCCAGCAGAGCCGGATGGCCTAGCATCAAAAAGCTTGATGATAAACAAGCTAAAGCCTACAGCGGTAAAAAGTGGTTTGAGGGTGATAGGTGGGTTAGGCCCAGCGGGGTTGCATTGCTTGGGAATAGTCTAGATGTAGAGTACTTCCTGCCTAGAGTACAAAGTAAGAAAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

611

Amino Acids

67.05

Weight (kDa)

8.57

Isoelectric Point (pI)

32.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	73 - 220	7.6e-25	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	291 - 582	5.9e-122	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1616
Acc36I	ACCTGC	2 cut(s)	998, 1403
Acc65I	GGTACC	1 cut(s)	1248
AccB1I	GGYRCC	4 cut(s)	1182, 1248, 1310, 1595
AccB7I	CCANNNNNTGG	1 cut(s)	1192
AccII	CGCG	3 cut(s)	120, 512, 1293
AclWI	GGATC	5 cut(s)	35, 88, 386, 843, 1067
AcoI	YGGCCR	3 cut(s)	63, 330, 1508
AcsI	RAATTY	3 cut(s)	459, 1129, 1519
AcuI	CTGAAG	1 cut(s)	1593
AcyI	GRCGYC	4 cut(s)	6, 712, 1596, 1613
AdeI	CACNNNGTG	1 cut(s)	1202
AfiI	CCNNNNNNNGG	3 cut(s)	721, 1192, 1592
AflII	CTTAAG	1 cut(s)	863
AflIII	ACRYGT	1 cut(s)	1656
AgsI	TTSAA	8 cut(s)	436, 457, 469, 622, 685, 928, 1208, 1568
AjnI	CCWGG	4 cut(s)	10, 720, 1055, 1645
AluBI	AGCT	8 cut(s)	343, 410, 430, 629, 848, 923, 1693, 1709
AluI	AGCT	8 cut(s)	343, 410, 430, 629, 848, 923, 1693, 1709
Alw21I	GWGCWC	1 cut(s)	1452
AlwI	GGATC	5 cut(s)	35, 88, 386, 843, 1067
AlwNI	CAGNNNCTG	1 cut(s)	1118
AoxI	GGCC	9 cut(s)	63, 279, 330, 384, 723, 1042, 1508, 1676, 1757
ApoI	RAATTY	3 cut(s)	459, 1129, 1519
ArsI	GACNNNNNNTTYG	6 cut(s)	1061, 1093, 1139, 1171, 1310, 1342
Asp700I	GAANNNNTTC	2 cut(s)	1286, 1520
Asp718I	GGTACC	1 cut(s)	1248
AspA2I	CCTAGG	1 cut(s)	1501
AspLEI	GCGC	3 cut(s)	55, 1598, 1652
AspS9I	GGNCC	4 cut(s)	934, 1043, 1643, 1758
AsuC2I	CCSGG	1 cut(s)	183
AsuHPI	GGTGA	8 cut(s)	542, 655, 1049, 1079, 1343, 1381, 1429, 1754
AsuNHI	GCTAGC	1 cut(s)	1082
AvaII	GGWCC	2 cut(s)	934, 1643
AvrII	CCTAGG	1 cut(s)	1501
BaeGI	GKGCMC	1 cut(s)	1185
BaeI	ACNNNNGTAYC	2 cut(s)	1489, 1522
BalI	TGGCCA	2 cut(s)	65, 332
BanI	GGYRCC	4 cut(s)	1182, 1248, 1310, 1595
Bbv12I	GWGCWC	1 cut(s)	1452
BccI	CCATC	7 cut(s)	745, 884, 920, 1009, 1034, 1472, 1668
BceAI	ACGGC	3 cut(s)	182, 699, 1609
BciT130I	CCWGG	4 cut(s)	12, 722, 1057, 1647
BciVI	GTATCC	2 cut(s)	315, 1490
BcnI	CCSGG	1 cut(s)	183
BfaI	CTAG	6 cut(s)	642, 1083, 1502, 1680, 1790, 1812
BfmI	CTRYAG	3 cut(s)	1119, 1276, 1717
BfoI	RGCGCY	3 cut(s)	56, 1599, 1653
BfrI	CTTAAG	1 cut(s)	863
BfuAI	ACCTGC	2 cut(s)	998, 1403
BfuI	GTATCC	2 cut(s)	315, 1490
BglII	AGATCT	1 cut(s)	487
BisI	GCNGC	1 cut(s)	734
BlnI	CCTAGG	1 cut(s)	1501
BlsI	GCNGC	1 cut(s)	735
BmcAI	AGTACT	1 cut(s)	1802
Bme1390I	CCNGG	5 cut(s)	12, 183, 722, 1057, 1647
Bme18I	GGWCC	2 cut(s)	934, 1643
BmgT120I	GGNCC	4 cut(s)	934, 1043, 1643, 1758
BmiI	GGNNCC	7 cut(s)	936, 1090, 1184, 1250, 1312, 1597, 1644
BmrFI	CCNGG	5 cut(s)	12, 183, 722, 1057, 1647
BmsI	GCATC	3 cut(s)	1407, 1452, 1692
BmtI	GCTAGC	1 cut(s)	1086
BpuEI	CTTGAG	1 cut(s)	870
BpuMI	CCSGG	1 cut(s)	183
BsaAI	YACGTR	1 cut(s)	970
BsaBI	GATNNNNATC	1 cut(s)	32
BsaHI	GRCGYC	4 cut(s)	6, 712, 1596, 1613
BsaI	GGTCTC	3 cut(s)	49, 288, 809
BsaJI	CCNNGG	8 cut(s)	10, 66, 657, 1143, 1411, 1501, 1511, 1586
BsaWI	WCCGGW	1 cut(s)	1064
BsaXI	ACNNNNNCTCC	4 cut(s)	702, 732, 968, 998
Bsc4I	CCNNNNNNNGG	3 cut(s)	721, 1192, 1592
Bse118I	RCCGGY	1 cut(s)	1439
Bse1I	ACTGG	2 cut(s)	936, 1312
Bse3DI	GCAATG	2 cut(s)	652, 1772
Bse8I	GATNNNNATC	1 cut(s)	32
BseBI	CCWGG	4 cut(s)	12, 722, 1057, 1647
BseDI	CCNNGG	8 cut(s)	10, 66, 657, 1143, 1411, 1501, 1511, 1586
BseGI	GGATG	3 cut(s)	352, 737, 1679
BseJI	GATNNNNATC	1 cut(s)	32
BseLI	CCNNNNNNNGG	3 cut(s)	721, 1192, 1592
BseMI	GCAATG	2 cut(s)	652, 1772
BseMII	CTCAG	2 cut(s)	524, 590
BseNI	ACTGG	2 cut(s)	936, 1312
BseRI	GAGGAG	2 cut(s)	150, 153
BseSI	GKGCMC	1 cut(s)	1185
BseYI	CCCAGC	1 cut(s)	1760
BsgI	GTGCAG	1 cut(s)	1444
Bsh1236I	CGCG	3 cut(s)	120, 512, 1293
BshFI	GGCC	9 cut(s)	65, 281, 332, 386, 725, 1044, 1510, 1678, 1759
BshNI	GGYRCC	4 cut(s)	1182, 1248, 1310, 1595
BsiHKAI	GWGCWC	1 cut(s)	1452
BsiSI	CCGG	6 cut(s)	183, 282, 1065, 1170, 1440, 1671
BslFI	GGGAC	3 cut(s)	920, 1604, 1656
BslI	CCNNNNNNNGG	3 cut(s)	721, 1192, 1592
BsmBI	CGTCTC	5 cut(s)	147, 153, 159, 279, 722
BsmFI	GGGAC	3 cut(s)	920, 1604, 1656
BsmI	GAATGC	1 cut(s)	442
BsnI	GGCC	9 cut(s)	65, 281, 332, 386, 725, 1044, 1510, 1678, 1759
Bso31I	GGTCTC	3 cut(s)	49, 288, 809
Bsp1286I	GDGCHC	2 cut(s)	1185, 1452
Bsp1407I	TGTACA	2 cut(s)	984, 1101
Bsp143I	GATC	8 cut(s)	27, 93, 379, 391, 487, 823, 835, 1059
Bsp19I	CCATGG	3 cut(s)	66, 657, 1511
BspANI	GGCC	9 cut(s)	65, 281, 332, 386, 725, 1044, 1510, 1678, 1759
BspCNI	CTCAG	2 cut(s)	525, 589
BspFNI	CGCG	3 cut(s)	120, 512, 1293
BspHI	TCATGA	1 cut(s)	631
BspLI	GGNNCC	7 cut(s)	936, 1090, 1184, 1250, 1312, 1597, 1644
BspMI	ACCTGC	2 cut(s)	998, 1403
BspOI	GCTAGC	1 cut(s)	1086
BspPI	GGATC	5 cut(s)	35, 88, 386, 843, 1067
BspQI	GCTCTTC	2 cut(s)	938, 1352
BspT107I	GGYRCC	4 cut(s)	1182, 1248, 1310, 1595
BspTI	CTTAAG	1 cut(s)	863
BspTNI	GGTCTC	3 cut(s)	49, 288, 809
BsrDI	GCAATG	2 cut(s)	652, 1772
BsrFI	RCCGGY	1 cut(s)	1439
BsrGI	TGTACA	2 cut(s)	984, 1101
BsrI	ACTGG	2 cut(s)	936, 1312
BssAI	RCCGGY	1 cut(s)	1439
BssECI	CCNNGG	8 cut(s)	10, 66, 657, 1143, 1411, 1501, 1511, 1586
BssMI	GATC	8 cut(s)	27, 93, 379, 391, 487, 823, 835, 1059
BssNI	GRCGYC	4 cut(s)	6, 712, 1596, 1613
BssT1I	CCWWGG	7 cut(s)	66, 657, 1143, 1411, 1501, 1511, 1586
Bst2UI	CCWGG	4 cut(s)	12, 722, 1057, 1647
Bst4CI	ACNGT	5 cut(s)	43, 880, 899, 1003, 1114
Bst6I	CTCTTC	4 cut(s)	52, 696, 938, 1352
BstACI	GRCGYC	4 cut(s)	6, 712, 1596, 1613
BstAFI	CTTAAG	1 cut(s)	863
BstAPI	GCANNNNNTGC	1 cut(s)	1115
BstAUI	TGTACA	2 cut(s)	984, 1101
BstBAI	YACGTR	1 cut(s)	970
BstC8I	GCNNGC	2 cut(s)	233, 1084
BstDEI	CTNAG	3 cut(s)	533, 576, 1050
BstDSI	CCRYGG	3 cut(s)	66, 657, 1511
BstEII	GGTNACC	2 cut(s)	661, 1435
BstF5I	GGATG	3 cut(s)	352, 737, 1679
BstFNI	CGCG	3 cut(s)	120, 512, 1293
BstH2I	RGCGCY	3 cut(s)	56, 1599, 1653
BstHHI	GCGC	3 cut(s)	55, 1598, 1652
BstKTI	GATC	8 cut(s)	30, 96, 382, 394, 490, 826, 838, 1062
BstMBI	GATC	8 cut(s)	27, 93, 379, 391, 487, 823, 835, 1059
BstMWI	GCNNNNNNNGC	1 cut(s)	1115
BstNI	CCWGG	4 cut(s)	12, 722, 1057, 1647
BstNSI	RCATGY	2 cut(s)	1585, 1660
BstPI	GGTNACC	2 cut(s)	661, 1435
BstSCI	CCNGG	5 cut(s)	10, 181, 720, 1055, 1645
BstSFI	CTRYAG	3 cut(s)	1119, 1276, 1717
BstSLI	GKGCMC	1 cut(s)	1185
BstUI	CGCG	3 cut(s)	120, 512, 1293
BstX2I	RGATCY	4 cut(s)	391, 487, 835, 1059
BstYI	RGATCY	4 cut(s)	391, 487, 835, 1059
BsuI	GTATCC	2 cut(s)	315, 1490
BsuRI	GGCC	9 cut(s)	65, 281, 332, 386, 725, 1044, 1510, 1678, 1759
BtgI	CCRYGG	3 cut(s)	66, 657, 1511
BtgZI	GCGATG	1 cut(s)	714
BtsCI	GGATG	3 cut(s)	352, 737, 1679
BtsI	GCAGTG	3 cut(s)	1104, 1196, 1404
BtsIMutI	CAGTG	5 cut(s)	819, 1104, 1196, 1305, 1404
BveI	ACCTGC	2 cut(s)	998, 1403
Cac8I	GCNNGC	2 cut(s)	233, 1084
CaiI	CAGNNNCTG	1 cut(s)	1118
CciI	TCATGA	1 cut(s)	631
CfoI	GCGC	3 cut(s)	55, 1598, 1652
Cfr10I	RCCGGY	1 cut(s)	1439
Cfr13I	GGNCC	4 cut(s)	934, 1043, 1643, 1758
CseI	GACGC	2 cut(s)	720, 1343
CviAII	CATG	9 cut(s)	67, 397, 632, 658, 1512, 1540, 1582, 1609, 1657
DdeI	CTNAG	3 cut(s)	533, 576, 1050
DinI	GGCGCC	1 cut(s)	1597
DpnI	GATC	8 cut(s)	29, 95, 381, 393, 489, 825, 837, 1061
DpnII	GATC	8 cut(s)	27, 93, 379, 391, 487, 823, 835, 1059
DraI	TTTAAA	1 cut(s)	700
DraIII	CACNNNGTG	1 cut(s)	1202
EaeI	YGGCCR	3 cut(s)	63, 330, 1508
Eam1104I	CTCTTC	4 cut(s)	52, 696, 938, 1352
EarI	CTCTTC	4 cut(s)	52, 696, 938, 1352
EciI	GGCGGA	3 cut(s)	141, 147, 159
Eco130I	CCWWGG	7 cut(s)	66, 657, 1143, 1411, 1501, 1511, 1586
Eco31I	GGTCTC	3 cut(s)	49, 288, 809
Eco47I	GGWCC	2 cut(s)	934, 1643
Eco57I	CTGAAG	1 cut(s)	1593
Eco91I	GGTNACC	2 cut(s)	661, 1435
EcoO109I	RGGNCCY	1 cut(s)	1643
EcoO65I	GGTNACC	2 cut(s)	661, 1435
EcoRII	CCWGG	4 cut(s)	10, 720, 1055, 1645
EcoT14I	CCWWGG	7 cut(s)	66, 657, 1143, 1411, 1501, 1511, 1586
EgeI	GGCGCC	1 cut(s)	1597
EheI	GGCGCC	1 cut(s)	1597
ErhI	CCWWGG	7 cut(s)	66, 657, 1143, 1411, 1501, 1511, 1586
Esp3I	CGTCTC	5 cut(s)	147, 153, 159, 279, 722
FaeI	CATG	9 cut(s)	70, 400, 635, 661, 1515, 1543, 1585, 1612, 1660
FalI	AAGNNNNNCTT	2 cut(s)	602, 634
FaqI	GGGAC	3 cut(s)	920, 1604, 1656
FatI	CATG	9 cut(s)	66, 396, 631, 657, 1511, 1539, 1581, 1608, 1656
FauI	CCCGC	1 cut(s)	1757
Fnu4HI	GCNGC	1 cut(s)	734
FokI	GGATG	3 cut(s)	359, 724, 1686
Fsp4HI	GCNGC	1 cut(s)	734
FspBI	CTAG	6 cut(s)	642, 1083, 1502, 1680, 1790, 1812
GlaI	GCGC	3 cut(s)	54, 1597, 1651
GluI	GCNGC	1 cut(s)	734
GsaI	CCCAGC	1 cut(s)	1764
HaeII	RGCGCY	3 cut(s)	56, 1599, 1653
HaeIII	GGCC	9 cut(s)	65, 281, 332, 386, 725, 1044, 1510, 1678, 1759
HapII	CCGG	6 cut(s)	183, 282, 1065, 1170, 1440, 1671
HgaI	GACGC	2 cut(s)	720, 1343
HhaI	GCGC	3 cut(s)	55, 1598, 1652
Hin1I	GRCGYC	4 cut(s)	6, 712, 1596, 1613
Hin1II	CATG	9 cut(s)	70, 400, 635, 661, 1515, 1543, 1585, 1612, 1660
Hin6I	GCGC	3 cut(s)	53, 1596, 1650
HinP1I	GCGC	3 cut(s)	53, 1596, 1650
HindIII	AAGCTT	2 cut(s)	921, 1691
HinfI	GANTC	3 cut(s)	173, 572, 1564
HpaII	CCGG	6 cut(s)	183, 282, 1065, 1170, 1440, 1671
HphI	GGTGA	8 cut(s)	542, 655, 1049, 1079, 1343, 1381, 1429, 1754
Hpy166II	GTNNAC	2 cut(s)	985, 1104
Hpy188I	TCNGA	4 cut(s)	579, 843, 1213, 1469
Hpy188III	TCNNGA	6 cut(s)	491, 619, 632, 887, 1351, 1790
Hpy8I	GTNNAC	2 cut(s)	985, 1104
Hpy99I	CGWCG	1 cut(s)	714
HpyAV	CCTTC	4 cut(s)	604, 819, 967, 1568
HpyCH4III	ACNGT	5 cut(s)	43, 880, 899, 1003, 1114
HpyCH4IV	ACGT	4 cut(s)	873, 969, 1266, 1613
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1115
HpyF3I	CTNAG	3 cut(s)	533, 576, 1050
HpySE526I	ACGT	4 cut(s)	873, 969, 1266, 1613
Hsp92I	GRCGYC	4 cut(s)	6, 712, 1596, 1613
Hsp92II	CATG	9 cut(s)	70, 400, 635, 661, 1515, 1543, 1585, 1612, 1660
HspAI	GCGC	3 cut(s)	53, 1596, 1650
KasI	GGCGCC	1 cut(s)	1595
KpnI	GGTACC	1 cut(s)	1252
Kzo9I	GATC	8 cut(s)	27, 93, 379, 391, 487, 823, 835, 1059
LguI	GCTCTTC	2 cut(s)	938, 1352
LmnI	GCTCC	3 cut(s)	112, 1172, 1455
LweI	GCATC	3 cut(s)	1407, 1452, 1692
MaeI	CTAG	6 cut(s)	642, 1083, 1502, 1680, 1790, 1812
MaeII	ACGT	4 cut(s)	873, 969, 1266, 1613
MaeIII	GTNAC	9 cut(s)	548, 661, 874, 997, 1067, 1331, 1402, 1435, 1545
MalI	GATC	8 cut(s)	29, 95, 381, 393, 489, 825, 837, 1061
MboI	GATC	8 cut(s)	27, 93, 379, 391, 487, 823, 835, 1059
MboII	GAAGA	8 cut(s)	39, 602, 683, 830, 955, 1318, 1339, 1528
MfeI	CAATTG	1 cut(s)	216
MflI	RGATCY	4 cut(s)	391, 487, 835, 1059
MhlI	GDGCHC	2 cut(s)	1185, 1452
MlsI	TGGCCA	2 cut(s)	65, 332
MluCI	AATT	5 cut(s)	216, 459, 1129, 1282, 1519
MluNI	TGGCCA	2 cut(s)	65, 332
Mly113I	GGCGCC	1 cut(s)	1596
MlyI	GAGTC	1 cut(s)	167
MmeI	TCCRAC	2 cut(s)	813, 1105
Mox20I	TGGCCA	2 cut(s)	65, 332
MroXI	GAANNNNTTC	2 cut(s)	1286, 1520
MscI	TGGCCA	2 cut(s)	65, 332
MseI	TTAA	3 cut(s)	336, 699, 864
Msp20I	TGGCCA	2 cut(s)	65, 332
MspA1I	CMGCKG	3 cut(s)	430, 1722, 1764
MspCI	CTTAAG	1 cut(s)	863
MspI	CCGG	6 cut(s)	183, 282, 1065, 1170, 1440, 1671
MspR9I	CCNGG	5 cut(s)	12, 183, 722, 1057, 1647
MunI	CAATTG	1 cut(s)	216
Mva1269I	GAATGC	1 cut(s)	442
MvaI	CCWGG	4 cut(s)	12, 722, 1057, 1647
MvnI	CGCG	3 cut(s)	120, 512, 1293
MwoI	GCNNNNNNNGC	1 cut(s)	1115
NarI	GGCGCC	1 cut(s)	1596
NciI	CCSGG	1 cut(s)	183
NcoI	CCATGG	3 cut(s)	66, 657, 1511
NdeII	GATC	8 cut(s)	27, 93, 379, 391, 487, 823, 835, 1059
NheI	GCTAGC	1 cut(s)	1082
NlaIII	CATG	9 cut(s)	70, 400, 635, 661, 1515, 1543, 1585, 1612, 1660
NlaIV	GGNNCC	7 cut(s)	936, 1090, 1184, 1250, 1312, 1597, 1644
NmuCI	GTSAC	9 cut(s)	548, 661, 874, 997, 1067, 1331, 1402, 1435, 1545
NspI	RCATGY	2 cut(s)	1585, 1660
PagI	TCATGA	1 cut(s)	631
PciI	ACATGT	1 cut(s)	1656
PciSI	GCTCTTC	2 cut(s)	938, 1352
PcsI	WCGNNNNNNNCGW	1 cut(s)	1629
PctI	GAATGC	1 cut(s)	442
PdmI	GAANNNNTTC	2 cut(s)	1286, 1520
PfeI	GAWTC	2 cut(s)	572, 1564
PflFI	GACNNNGTC	2 cut(s)	714, 1337
PflMI	CCANNNNNTGG	1 cut(s)	1192
PfoI	TCCNGGA	1 cut(s)	1055
PkrI	GCNGC	1 cut(s)	735
PleI	GAGTC	1 cut(s)	167
PluTI	GGCGCC	1 cut(s)	1599
PpsI	GAGTC	1 cut(s)	167
Ppu21I	YACGTR	1 cut(s)	970
PpuMI	RGGWCCY	1 cut(s)	1643
PscI	ACATGT	1 cut(s)	1656
Psp5II	RGGWCCY	1 cut(s)	1643
Psp6I	CCWGG	4 cut(s)	10, 720, 1055, 1645
PspEI	GGTNACC	2 cut(s)	661, 1435
PspFI	CCCAGC	1 cut(s)	1760
PspGI	CCWGG	4 cut(s)	10, 720, 1055, 1645
PspN4I	GGNNCC	7 cut(s)	936, 1090, 1184, 1250, 1312, 1597, 1644
PspPI	GGNCC	4 cut(s)	934, 1043, 1643, 1758
PspPPI	RGGWCCY	1 cut(s)	1643
PsrI	GAACNNNNNNTAC	2 cut(s)	1481, 1513
PstNI	CAGNNNCTG	1 cut(s)	1118
PsuI	RGATCY	4 cut(s)	391, 487, 835, 1059
PsyI	GACNNNGTC	2 cut(s)	714, 1337
PvuII	CAGCTG	1 cut(s)	430
SapI	GCTCTTC	2 cut(s)	938, 1352
SaqAI	TTAA	3 cut(s)	336, 699, 864
SatI	GCNGC	1 cut(s)	734
Sau3AI	GATC	8 cut(s)	27, 93, 379, 391, 487, 823, 835, 1059
Sau96I	GGNCC	4 cut(s)	934, 1043, 1643, 1758
ScaI	AGTACT	1 cut(s)	1802
SchI	GAGTC	1 cut(s)	167
ScrFI	CCNGG	5 cut(s)	12, 183, 722, 1057, 1647
SduI	GDGCHC	2 cut(s)	1185, 1452
SfaNI	GCATC	3 cut(s)	1407, 1452, 1692
SfcI	CTRYAG	3 cut(s)	1119, 1276, 1717
SfoI	GGCGCC	1 cut(s)	1597
SinI	GGWCC	2 cut(s)	934, 1643
SmlI	CTYRAG	2 cut(s)	863, 885
SmoI	CTYRAG	2 cut(s)	863, 885
Sse9I	AATT	5 cut(s)	216, 459, 1129, 1282, 1519
SspDI	GGCGCC	1 cut(s)	1595
SspMI	CTAG	6 cut(s)	642, 1083, 1502, 1680, 1790, 1812
StyD4I	CCNGG	5 cut(s)	10, 181, 720, 1055, 1645
StyI	CCWWGG	7 cut(s)	66, 657, 1143, 1411, 1501, 1511, 1586
TaaI	ACNGT	5 cut(s)	43, 880, 899, 1003, 1114
TaiI	ACGT	4 cut(s)	876, 972, 1269, 1616
TaqI	TCGA	2 cut(s)	492, 704
TasI	AATT	5 cut(s)	216, 459, 1129, 1282, 1519
TatI	WGTACW	4 cut(s)	984, 1101, 1800, 1816
TauI	GCSGC	1 cut(s)	736
TfiI	GAWTC	2 cut(s)	572, 1564
Tru1I	TTAA	3 cut(s)	336, 699, 864
Tru9I	TTAA	3 cut(s)	336, 699, 864
TscAI	CASTG	5 cut(s)	826, 1111, 1203, 1312, 1411
TseFI	GTSAC	9 cut(s)	548, 661, 874, 997, 1067, 1331, 1402, 1435, 1545
Tsp45I	GTSAC	9 cut(s)	548, 661, 874, 997, 1067, 1331, 1402, 1435, 1545
TspDTI	ATGAA	6 cut(s)	519, 585, 623, 648, 683, 1312
TspGWI	ACGGA	4 cut(s)	171, 177, 183, 191
TspRI	CASTG	5 cut(s)	826, 1111, 1203, 1312, 1411
Tth111I	GACNNNGTC	2 cut(s)	714, 1337
Van91I	CCANNNNNTGG	1 cut(s)	1192
Vha464I	CTTAAG	1 cut(s)	863
VpaK11BI	GGWCC	2 cut(s)	934, 1643
XapI	RAATTY	3 cut(s)	459, 1129, 1519
XbaI	TCTAGA	1 cut(s)	1789
XceI	RCATGY	2 cut(s)	1585, 1660
XcmI	CCANNNNNNNNNTGG	2 cut(s)	340, 877
XmaJI	CCTAGG	1 cut(s)	1501
XmnI	GAANNNNTTC	2 cut(s)	1286, 1520
XspI	CTAG	6 cut(s)	642, 1083, 1502, 1680, 1790, 1812
ZraI	GACGTC	1 cut(s)	1614
ZrmI	AGTACT	1 cut(s)	1802

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.