Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Forward (+)

18206965 .. 18209372

Gene Structure 2408 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000007564

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1794 bp

ATGGGCATCCCTGGTGGTAAGAAGAGGATCATAATCGTTACGGTCTCTTCAGCGCTTCTGGTGGCCATGGTTGTCGGTGCGGTGGTTGGTGTGATCCAATACAAAAAACAGGAGCAACGTGGAGGCGGAGGAGGAGGCGGAGGCGGAGACGGAGACGGAGACGGAGACGGAGACTCTAAACCTGGAGATAAAAACGCCGTCCGTACATCAAAGCCAATTGTAGCTATTTGCCAACCCACCAACTACAAACAAGAATGTGAGAATAGTCTGCAAAAGGAGGCCGGAGATAAAAGAGACCCGAAAGATCTCGCAAGAGCGGGGATACAGGTGGCCATTAACAAACTGGATGAGGCTATAAGGAACTCCAAAACCTTGCCAGATCAGGCCAAAGATCCCATGACCAAACAAGCTCTCCATATCTGCAACCAGCTGTTGAATGCTTCTATGTACGATTTGAAAATTTCTTTCAATAAAATGGGTGCATTAGATCTCGAAAAGGTTGATGAATACGCGAAAGATGTTGAGGTTTGGCTGAGTGGGGCTGTCAGCCACCAAGCGACTTGCATTGATGAATCTCAGAACACAACCGAAGATGTCGGCAAGAAAATGAAGGAAATTTTGAAGTCAGCTCATGAAAGCACTAGCAATGCACTTGCCATGGTCACCGAAATCACTAACGCCTTGAACTCTCTTCATTTTAAATCGAACCGACGCCGGCTCCTGGCCTCATCGCCGCCATCCAAGAATAACAACAATAATAAGAATAATCAACACAAGCAGAATGGCAACAAAAGTAATCAGGGTCTCCCTTGTTGGCTTACTGGTCGTAAGTTGGATCTTCTGACAGCTACGCCTGAAACCCTTAAGCCCAACGTGACGGTCGCTCAAGATGGCAACACTAAGTATAAAACCATCAACGAAGCTTTGAAACTGGTCCCAAAGAAGAGCAACGACTTGTTTGTGATTTACGTGAAGGAGGGAGTGTACAAAGAAAGTGTGACGGTTGCAGGTATGATGGAAAATGTTGTGATGATTGGTGATGGCCCTACTAAGTCCAGGATCTCCGGTGACAATAACTTTGCTAGCGGAACCAGAACTATGTACACTGCAACAGTTGCTGTAGTTGGAAATTTTTTTGTCGCCAAGGACATAGGGTTTGAGAACACAGCCGGAGCGAAAGGGCACCAAGCGGTGGCACTGCGCGTTCAATCGGACTTCTCCATCTTCTACAACTGCCACATTGACGGGTACCAAAACACCCTATACGTCCAAGCCTACAGGAATTACTTCCGCGACTGCAACATCACTGGCACCATTGACTTCATCTTCGGTGACGCTGTCTCTCTCTTCCAGAACTGCGAGATTGTGGTGAGGAAGCCAATGGACAACCAGACCTGCATCGTCACTGCCCAAGGAAAGACAGACAAGAATGAGGTCACCGGCATCGTGCTTCAAAACTGCACCATCACAGGCGACCCAGAGTACGAGAACAAGGGATACCTAGGTCGGCCATGGAAGAACTTCGCAAAAACCGTTGTCATGCAGTCACAGATTGATGATGTGATTCAACCTGAAGGGTACATGCCTTGGGACGGCGCCAAGTTCCACATGACGTCATTTTTCGGGGAGTACGACAACAGGGGACCTGGCGCTGACATGTCCAGCAGAGCCGGATGGCCTGGCATCAAAAAGCTTGATGATAAACAAGCTAAAGCCTACAGCGGTAAAAAGTGGTTTGAGGGTGATAGGTGGGTTAAGCCCAGCGGGGTCCCTTTTGTTGCTGGCATCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

598

Amino Acids

65.27

Weight (kDa)

8.72

Isoelectric Point (pI)

30.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	73 - 220	7.2e-25	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	291 - 582	7.7e-123	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1616
Acc36I	ACCTGC	2 cut(s)	998, 1403
Acc65I	GGTACC	1 cut(s)	1248
AccB1I	GGYRCC	4 cut(s)	1182, 1248, 1310, 1595
AccB7I	CCANNNNNTGG	1 cut(s)	1192
AccBSI	CCGCTC	1 cut(s)	317
AccII	CGCG	3 cut(s)	512, 1203, 1293
AclWI	GGATC	5 cut(s)	35, 88, 386, 843, 1067
AcoI	YGGCCR	3 cut(s)	63, 330, 1508
AcsI	RAATTY	3 cut(s)	459, 615, 1129
AcuI	CTGAAG	2 cut(s)	33, 1593
AcyI	GRCGYC	3 cut(s)	712, 1596, 1613
AfaI	GTAC	8 cut(s)	205, 449, 986, 1103, 1250, 1484, 1580, 1631
AfeI	AGCGCT	1 cut(s)	54
AfiI	CCNNNNNNNGG	3 cut(s)	721, 1192, 1592
AflII	CTTAAG	1 cut(s)	863
AflIII	ACRYGT	1 cut(s)	1656
AgsI	TTSAA	9 cut(s)	436, 457, 469, 622, 685, 928, 1208, 1454, 1568
AjnI	CCWGG	6 cut(s)	10, 181, 720, 1055, 1645, 1678
AluBI	AGCT	8 cut(s)	224, 410, 430, 629, 848, 923, 1693, 1709
AluI	AGCT	8 cut(s)	224, 410, 430, 629, 848, 923, 1693, 1709
Alw26I	GTCTC	9 cut(s)	49, 141, 147, 153, 159, 165, 288, 809, 1345
AlwI	GGATC	5 cut(s)	35, 88, 386, 843, 1067
AlwNI	CAGNNNCTG	1 cut(s)	1118
Aor51HI	AGCGCT	1 cut(s)	54
AoxI	GGCC	8 cut(s)	63, 279, 330, 384, 723, 1042, 1508, 1676
ApoI	RAATTY	3 cut(s)	459, 615, 1129
ArsI	GACNNNNNNTTYG	6 cut(s)	1061, 1093, 1139, 1171, 1310, 1342
Asp700I	GAANNNNTTC	2 cut(s)	1286, 1520
Asp718I	GGTACC	1 cut(s)	1248
AspA2I	CCTAGG	1 cut(s)	1501
AspLEI	GCGC	4 cut(s)	55, 1203, 1598, 1652
AspS9I	GGNCC	4 cut(s)	934, 1043, 1643, 1768
AsuHPI	GGTGA	7 cut(s)	655, 1049, 1079, 1343, 1381, 1429, 1754
AsuNHI	GCTAGC	1 cut(s)	1082
AvaII	GGWCC	3 cut(s)	934, 1643, 1768
AvrII	CCTAGG	1 cut(s)	1501
BaeGI	GKGCMC	1 cut(s)	1185
BaeI	ACNNNNGTAYC	2 cut(s)	1489, 1522
BalI	TGGCCA	2 cut(s)	65, 332
BanI	GGYRCC	4 cut(s)	1182, 1248, 1310, 1595
BccI	CCATC	8 cut(s)	745, 884, 920, 1009, 1034, 1229, 1472, 1668
BceAI	ACGGC	2 cut(s)	182, 1609
BciT130I	CCWGG	6 cut(s)	12, 183, 722, 1057, 1647, 1680
BciVI	GTATCC	2 cut(s)	315, 1490
BcoDI	GTCTC	9 cut(s)	49, 141, 147, 153, 159, 165, 288, 809, 1345
BfaI	CTAG	3 cut(s)	642, 1083, 1502
BfmI	CTRYAG	3 cut(s)	1119, 1276, 1717
BfoI	RGCGCY	3 cut(s)	56, 1599, 1653
BfrI	CTTAAG	1 cut(s)	863
BfuAI	ACCTGC	2 cut(s)	998, 1403
BfuI	GTATCC	2 cut(s)	315, 1490
BglII	AGATCT	2 cut(s)	304, 487
BisI	GCNGC	1 cut(s)	734
BlnI	CCTAGG	1 cut(s)	1501
BlsI	GCNGC	1 cut(s)	735
Bme1390I	CCNGG	6 cut(s)	12, 183, 722, 1057, 1647, 1680
Bme18I	GGWCC	3 cut(s)	934, 1643, 1768
BmgT120I	GGNCC	4 cut(s)	934, 1043, 1643, 1768
BmrFI	CCNGG	6 cut(s)	12, 183, 722, 1057, 1647, 1680
BmsI	GCATC	4 cut(s)	15, 1407, 1452, 1692
BmtI	GCTAGC	1 cut(s)	1086
BpmI	CTGGAG	1 cut(s)	204
BpuEI	CTTGAG	1 cut(s)	870
BsaAI	YACGTR	1 cut(s)	970
BsaBI	GATNNNNATC	1 cut(s)	32
BsaHI	GRCGYC	3 cut(s)	712, 1596, 1613
BsaI	GGTCTC	3 cut(s)	49, 288, 809
BsaJI	CCNNGG	8 cut(s)	10, 66, 657, 1143, 1411, 1501, 1511, 1586
BsaWI	WCCGGW	1 cut(s)	1064
BsaXI	ACNNNNNCTCC	6 cut(s)	396, 426, 702, 732, 968, 998
Bsc4I	CCNNNNNNNGG	3 cut(s)	721, 1192, 1592
Bse118I	RCCGGY	2 cut(s)	714, 1439
Bse1I	ACTGG	4 cut(s)	348, 826, 936, 1312
Bse3DI	GCAATG	1 cut(s)	652
Bse8I	GATNNNNATC	1 cut(s)	32
BseBI	CCWGG	6 cut(s)	12, 183, 722, 1057, 1647, 1680
BseDI	CCNNGG	8 cut(s)	10, 66, 657, 1143, 1411, 1501, 1511, 1586
BseGI	GGATG	4 cut(s)	6, 352, 737, 1679
BseJI	GATNNNNATC	1 cut(s)	32
BseLI	CCNNNNNNNGG	3 cut(s)	721, 1192, 1592
BseMI	GCAATG	1 cut(s)	652
BseMII	CTCAG	2 cut(s)	524, 590
BseNI	ACTGG	4 cut(s)	348, 826, 936, 1312
BseRI	GAGGAG	2 cut(s)	144, 147
BseSI	GKGCMC	1 cut(s)	1185
BseYI	CCCAGC	1 cut(s)	1760
BsgI	GTGCAG	1 cut(s)	1444
Bsh1236I	CGCG	3 cut(s)	512, 1203, 1293
Bsh1285I	CGRYCG	1 cut(s)	882
BshFI	GGCC	8 cut(s)	65, 281, 332, 386, 725, 1044, 1510, 1678
BshNI	GGYRCC	4 cut(s)	1182, 1248, 1310, 1595
BsiEI	CGRYCG	1 cut(s)	882
BsiSI	CCGG	6 cut(s)	282, 715, 1065, 1170, 1440, 1671
BslFI	GGGAC	4 cut(s)	920, 1604, 1656, 1754
BslI	CCNNNNNNNGG	3 cut(s)	721, 1192, 1592
BsmAI	GTCTC	9 cut(s)	49, 141, 147, 153, 159, 165, 288, 809, 1345
BsmBI	CGTCTC	4 cut(s)	141, 147, 153, 159
BsmFI	GGGAC	4 cut(s)	920, 1604, 1656, 1754
BsmI	GAATGC	1 cut(s)	442
BsnI	GGCC	8 cut(s)	65, 281, 332, 386, 725, 1044, 1510, 1678
Bso31I	GGTCTC	3 cut(s)	49, 288, 809
Bsp1286I	GDGCHC	1 cut(s)	1185
Bsp1407I	TGTACA	2 cut(s)	984, 1101
Bsp143I	GATC	8 cut(s)	27, 93, 304, 379, 391, 487, 835, 1059
Bsp19I	CCATGG	3 cut(s)	66, 657, 1511
BspANI	GGCC	8 cut(s)	65, 281, 332, 386, 725, 1044, 1510, 1678
BspCNI	CTCAG	2 cut(s)	525, 589
BspFNI	CGCG	3 cut(s)	512, 1203, 1293
BspHI	TCATGA	1 cut(s)	631
BspMI	ACCTGC	2 cut(s)	998, 1403
BspOI	GCTAGC	1 cut(s)	1086
BspPI	GGATC	5 cut(s)	35, 88, 386, 843, 1067
BspQI	GCTCTTC	1 cut(s)	938
BspT107I	GGYRCC	4 cut(s)	1182, 1248, 1310, 1595
BspTI	CTTAAG	1 cut(s)	863
BspTNI	GGTCTC	3 cut(s)	49, 288, 809
BsrBI	CCGCTC	1 cut(s)	317
BsrDI	GCAATG	1 cut(s)	652
BsrFI	RCCGGY	2 cut(s)	714, 1439
BsrGI	TGTACA	2 cut(s)	984, 1101
BsrI	ACTGG	4 cut(s)	348, 826, 936, 1312
BssAI	RCCGGY	2 cut(s)	714, 1439
BssECI	CCNNGG	8 cut(s)	10, 66, 657, 1143, 1411, 1501, 1511, 1586
BssMI	GATC	8 cut(s)	27, 93, 304, 379, 391, 487, 835, 1059
BssNI	GRCGYC	3 cut(s)	712, 1596, 1613
BssT1I	CCWWGG	7 cut(s)	66, 657, 1143, 1411, 1501, 1511, 1586
Bst2UI	CCWGG	6 cut(s)	12, 183, 722, 1057, 1647, 1680
Bst4CI	ACNGT	5 cut(s)	43, 880, 1003, 1114, 1534
Bst6I	CTCTTC	5 cut(s)	17, 52, 696, 938, 1352
BstACI	GRCGYC	3 cut(s)	712, 1596, 1613
BstAFI	CTTAAG	1 cut(s)	863
BstAPI	GCANNNNNTGC	1 cut(s)	1115
BstAUI	TGTACA	2 cut(s)	984, 1101
BstBAI	YACGTR	1 cut(s)	970
BstC8I	GCNNGC	3 cut(s)	716, 1084, 1783
BstDEI	CTNAG	4 cut(s)	533, 576, 900, 1050
BstDSI	CCRYGG	3 cut(s)	66, 657, 1511
BstEII	GGTNACC	2 cut(s)	661, 1435
BstF5I	GGATG	4 cut(s)	6, 352, 737, 1679
BstFNI	CGCG	3 cut(s)	512, 1203, 1293
BstH2I	RGCGCY	3 cut(s)	56, 1599, 1653
BstHHI	GCGC	4 cut(s)	55, 1203, 1598, 1652
BstKTI	GATC	8 cut(s)	30, 96, 307, 382, 394, 490, 838, 1062
BstMAI	GTCTC	9 cut(s)	49, 141, 147, 153, 159, 165, 288, 809, 1345
BstMBI	GATC	8 cut(s)	27, 93, 304, 379, 391, 487, 835, 1059
BstMCI	CGRYCG	1 cut(s)	882
BstMWI	GCNNNNNNNGC	1 cut(s)	1115
BstNI	CCWGG	6 cut(s)	12, 183, 722, 1057, 1647, 1680
BstNSI	RCATGY	2 cut(s)	1585, 1660
BstPI	GGTNACC	2 cut(s)	661, 1435
BstSCI	CCNGG	6 cut(s)	10, 181, 720, 1055, 1645, 1678
BstSFI	CTRYAG	3 cut(s)	1119, 1276, 1717
BstSLI	GKGCMC	1 cut(s)	1185
BstUI	CGCG	3 cut(s)	512, 1203, 1293
BstX2I	RGATCY	5 cut(s)	304, 391, 487, 835, 1059
BstYI	RGATCY	5 cut(s)	304, 391, 487, 835, 1059
BsuI	GTATCC	2 cut(s)	315, 1490
BsuRI	GGCC	8 cut(s)	65, 281, 332, 386, 725, 1044, 1510, 1678
BtgI	CCRYGG	3 cut(s)	66, 657, 1511
BtgZI	GCGATG	1 cut(s)	714
BtsCI	GGATG	4 cut(s)	6, 352, 737, 1679
BtsI	GCAGTG	3 cut(s)	1104, 1196, 1404
BtsIMutI	CAGTG	4 cut(s)	1104, 1196, 1305, 1404
BveI	ACCTGC	2 cut(s)	998, 1403
Cac8I	GCNNGC	3 cut(s)	716, 1084, 1783
CaiI	CAGNNNCTG	1 cut(s)	1118
CciI	TCATGA	1 cut(s)	631
CfoI	GCGC	4 cut(s)	55, 1203, 1598, 1652
Cfr10I	RCCGGY	2 cut(s)	714, 1439
Cfr13I	GGNCC	4 cut(s)	934, 1043, 1643, 1768
CseI	GACGC	2 cut(s)	720, 1343
Csp6I	GTAC	8 cut(s)	204, 448, 985, 1102, 1249, 1483, 1579, 1630
CviQI	GTAC	8 cut(s)	204, 448, 985, 1102, 1249, 1483, 1579, 1630
DdeI	CTNAG	4 cut(s)	533, 576, 900, 1050
DinI	GGCGCC	1 cut(s)	1597
DpnI	GATC	8 cut(s)	29, 95, 306, 381, 393, 489, 837, 1061
DpnII	GATC	8 cut(s)	27, 93, 304, 379, 391, 487, 835, 1059
DraI	TTTAAA	1 cut(s)	700
EaeI	YGGCCR	3 cut(s)	63, 330, 1508
Eam1104I	CTCTTC	5 cut(s)	17, 52, 696, 938, 1352
EarI	CTCTTC	5 cut(s)	17, 52, 696, 938, 1352
EciI	GGCGGA	3 cut(s)	141, 153, 159
Eco130I	CCWWGG	7 cut(s)	66, 657, 1143, 1411, 1501, 1511, 1586
Eco31I	GGTCTC	3 cut(s)	49, 288, 809
Eco47I	GGWCC	3 cut(s)	934, 1643, 1768
Eco47III	AGCGCT	1 cut(s)	54
Eco57I	CTGAAG	2 cut(s)	33, 1593
Eco91I	GGTNACC	2 cut(s)	661, 1435
EcoO109I	RGGNCCY	2 cut(s)	1643, 1768
EcoO65I	GGTNACC	2 cut(s)	661, 1435
EcoRII	CCWGG	6 cut(s)	10, 181, 720, 1055, 1645, 1678
EcoT14I	CCWWGG	7 cut(s)	66, 657, 1143, 1411, 1501, 1511, 1586
EgeI	GGCGCC	1 cut(s)	1597
EheI	GGCGCC	1 cut(s)	1597
ErhI	CCWWGG	7 cut(s)	66, 657, 1143, 1411, 1501, 1511, 1586
Esp3I	CGTCTC	4 cut(s)	141, 147, 153, 159
FaqI	GGGAC	4 cut(s)	920, 1604, 1656, 1754
FauI	CCCGC	2 cut(s)	310, 1757
Fnu4HI	GCNGC	1 cut(s)	734
FokI	GGATG	3 cut(s)	359, 724, 1686
Fsp4HI	GCNGC	1 cut(s)	734
FspBI	CTAG	3 cut(s)	642, 1083, 1502
GlaI	GCGC	4 cut(s)	54, 1202, 1597, 1651
GluI	GCNGC	1 cut(s)	734
GsaI	CCCAGC	1 cut(s)	1764
GsuI	CTGGAG	1 cut(s)	204
HaeII	RGCGCY	3 cut(s)	56, 1599, 1653
HaeIII	GGCC	8 cut(s)	65, 281, 332, 386, 725, 1044, 1510, 1678
HapII	CCGG	6 cut(s)	282, 715, 1065, 1170, 1440, 1671
HgaI	GACGC	2 cut(s)	720, 1343
HhaI	GCGC	4 cut(s)	55, 1203, 1598, 1652
Hin1I	GRCGYC	3 cut(s)	712, 1596, 1613
Hin6I	GCGC	4 cut(s)	53, 1201, 1596, 1650
HinP1I	GCGC	4 cut(s)	53, 1201, 1596, 1650
HindIII	AAGCTT	2 cut(s)	921, 1691
HinfI	GANTC	3 cut(s)	173, 572, 1564
HpaII	CCGG	6 cut(s)	282, 715, 1065, 1170, 1440, 1671
HphI	GGTGA	7 cut(s)	655, 1049, 1079, 1343, 1381, 1429, 1754
Hpy166II	GTNNAC	2 cut(s)	985, 1104
Hpy188I	TCNGA	3 cut(s)	579, 843, 1213
Hpy188III	TCNNGA	4 cut(s)	491, 632, 887, 1351
Hpy8I	GTNNAC	2 cut(s)	985, 1104
Hpy99I	CGWCG	1 cut(s)	714
HpyAV	CCTTC	3 cut(s)	604, 967, 1568
HpyCH4III	ACNGT	5 cut(s)	43, 880, 1003, 1114, 1534
HpyCH4IV	ACGT	5 cut(s)	118, 873, 969, 1266, 1613
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1115
HpyF3I	CTNAG	4 cut(s)	533, 576, 900, 1050
HpySE526I	ACGT	5 cut(s)	118, 873, 969, 1266, 1613
Hsp92I	GRCGYC	3 cut(s)	712, 1596, 1613
HspAI	GCGC	4 cut(s)	53, 1201, 1596, 1650
KasI	GGCGCC	1 cut(s)	1595
KflI	GGGWCCC	1 cut(s)	1768
KpnI	GGTACC	1 cut(s)	1252
KroI	GCCGGC	1 cut(s)	714
KroNI	GCCGGC	1 cut(s)	716
Kzo9I	GATC	8 cut(s)	27, 93, 304, 379, 391, 487, 835, 1059
LguI	GCTCTTC	1 cut(s)	938
LmnI	GCTCC	3 cut(s)	112, 723, 1172
LweI	GCATC	4 cut(s)	15, 1407, 1452, 1692
MaeI	CTAG	3 cut(s)	642, 1083, 1502
MaeII	ACGT	5 cut(s)	118, 873, 969, 1266, 1613
MaeIII	GTNAC	9 cut(s)	37, 661, 874, 997, 1067, 1331, 1402, 1435, 1545
MalI	GATC	8 cut(s)	29, 95, 306, 381, 393, 489, 837, 1061
MbiI	CCGCTC	1 cut(s)	317
MboI	GATC	8 cut(s)	27, 93, 304, 379, 391, 487, 835, 1059
MfeI	CAATTG	1 cut(s)	216
MflI	RGATCY	5 cut(s)	304, 391, 487, 835, 1059
MhlI	GDGCHC	1 cut(s)	1185
MlsI	TGGCCA	2 cut(s)	65, 332
MluCI	AATT	5 cut(s)	216, 459, 615, 1129, 1282
MluNI	TGGCCA	2 cut(s)	65, 332
Mly113I	GGCGCC	1 cut(s)	1596
MlyI	GAGTC	1 cut(s)	167
MmeI	TCCRAC	2 cut(s)	813, 1105
Mox20I	TGGCCA	2 cut(s)	65, 332
MroNI	GCCGGC	1 cut(s)	714
MroXI	GAANNNNTTC	2 cut(s)	1286, 1520
MscI	TGGCCA	2 cut(s)	65, 332
MseI	TTAA	4 cut(s)	336, 699, 864, 1755
Msp20I	TGGCCA	2 cut(s)	65, 332
MspA1I	CMGCKG	3 cut(s)	430, 1722, 1764
MspCI	CTTAAG	1 cut(s)	863
MspI	CCGG	6 cut(s)	282, 715, 1065, 1170, 1440, 1671
MspR9I	CCNGG	6 cut(s)	12, 183, 722, 1057, 1647, 1680
MunI	CAATTG	1 cut(s)	216
Mva1269I	GAATGC	1 cut(s)	442
MvaI	CCWGG	6 cut(s)	12, 183, 722, 1057, 1647, 1680
MvnI	CGCG	3 cut(s)	512, 1203, 1293
MwoI	GCNNNNNNNGC	1 cut(s)	1115
NaeI	GCCGGC	1 cut(s)	716
NarI	GGCGCC	1 cut(s)	1596
NcoI	CCATGG	3 cut(s)	66, 657, 1511
NdeII	GATC	8 cut(s)	27, 93, 304, 379, 391, 487, 835, 1059
NgoMIV	GCCGGC	1 cut(s)	714
NheI	GCTAGC	1 cut(s)	1082
NmuCI	GTSAC	8 cut(s)	661, 874, 997, 1067, 1331, 1402, 1435, 1545
NspI	RCATGY	2 cut(s)	1585, 1660
PagI	TCATGA	1 cut(s)	631
PciI	ACATGT	1 cut(s)	1656
PciSI	GCTCTTC	1 cut(s)	938
PcsI	WCGNNNNNNNCGW	2 cut(s)	1530, 1629
PctI	GAATGC	1 cut(s)	442
PdiI	GCCGGC	1 cut(s)	716
PdmI	GAANNNNTTC	2 cut(s)	1286, 1520
PfeI	GAWTC	2 cut(s)	572, 1564
PflFI	GACNNNGTC	1 cut(s)	1337
PflMI	CCANNNNNTGG	1 cut(s)	1192
PfoI	TCCNGGA	1 cut(s)	1055
PkrI	GCNGC	1 cut(s)	735
PleI	GAGTC	1 cut(s)	167
PluTI	GGCGCC	1 cut(s)	1599
PpsI	GAGTC	1 cut(s)	167
Ppu21I	YACGTR	1 cut(s)	970
PpuMI	RGGWCCY	2 cut(s)	1643, 1768
PscI	ACATGT	1 cut(s)	1656
Psp5II	RGGWCCY	2 cut(s)	1643, 1768
Psp6I	CCWGG	6 cut(s)	10, 181, 720, 1055, 1645, 1678
PspEI	GGTNACC	2 cut(s)	661, 1435
PspFI	CCCAGC	1 cut(s)	1760
PspGI	CCWGG	6 cut(s)	10, 181, 720, 1055, 1645, 1678
PspPI	GGNCC	4 cut(s)	934, 1043, 1643, 1768
PspPPI	RGGWCCY	2 cut(s)	1643, 1768
PsrI	GAACNNNNNNTAC	2 cut(s)	1481, 1513
PstNI	CAGNNNCTG	1 cut(s)	1118
PsuI	RGATCY	5 cut(s)	304, 391, 487, 835, 1059
PsyI	GACNNNGTC	1 cut(s)	1337
PvuII	CAGCTG	1 cut(s)	430
RsaI	GTAC	8 cut(s)	205, 449, 986, 1103, 1250, 1484, 1580, 1631
RsaNI	GTAC	8 cut(s)	204, 448, 985, 1102, 1249, 1483, 1579, 1630
SapI	GCTCTTC	1 cut(s)	938
SaqAI	TTAA	4 cut(s)	336, 699, 864, 1755
SatI	GCNGC	1 cut(s)	734
Sau3AI	GATC	8 cut(s)	27, 93, 304, 379, 391, 487, 835, 1059
Sau96I	GGNCC	4 cut(s)	934, 1043, 1643, 1768
SchI	GAGTC	1 cut(s)	167
ScrFI	CCNGG	6 cut(s)	12, 183, 722, 1057, 1647, 1680
SduI	GDGCHC	1 cut(s)	1185
SfaNI	GCATC	4 cut(s)	15, 1407, 1452, 1692
SfcI	CTRYAG	3 cut(s)	1119, 1276, 1717
SfoI	GGCGCC	1 cut(s)	1597
SinI	GGWCC	3 cut(s)	934, 1643, 1768
SmlI	CTYRAG	2 cut(s)	863, 885
SmoI	CTYRAG	2 cut(s)	863, 885
Sse9I	AATT	5 cut(s)	216, 459, 615, 1129, 1282
SspDI	GGCGCC	1 cut(s)	1595
SspMI	CTAG	3 cut(s)	642, 1083, 1502
StyD4I	CCNGG	6 cut(s)	10, 181, 720, 1055, 1645, 1678
StyI	CCWWGG	7 cut(s)	66, 657, 1143, 1411, 1501, 1511, 1586
TaaI	ACNGT	5 cut(s)	43, 880, 1003, 1114, 1534
TaiI	ACGT	5 cut(s)	121, 876, 972, 1269, 1616
TaqI	TCGA	2 cut(s)	492, 704
TasI	AATT	5 cut(s)	216, 459, 615, 1129, 1282
TatI	WGTACW	2 cut(s)	984, 1101
TauI	GCSGC	1 cut(s)	736
TfiI	GAWTC	2 cut(s)	572, 1564
Tru1I	TTAA	4 cut(s)	336, 699, 864, 1755
Tru9I	TTAA	4 cut(s)	336, 699, 864, 1755
TscAI	CASTG	4 cut(s)	1111, 1203, 1312, 1411
TseFI	GTSAC	8 cut(s)	661, 874, 997, 1067, 1331, 1402, 1435, 1545
Tsp45I	GTSAC	8 cut(s)	661, 874, 997, 1067, 1331, 1402, 1435, 1545
TspDTI	ATGAA	6 cut(s)	519, 585, 623, 648, 683, 1312
TspGWI	ACGGA	5 cut(s)	165, 171, 177, 183, 191
TspRI	CASTG	4 cut(s)	1111, 1203, 1312, 1411
Tth111I	GACNNNGTC	1 cut(s)	1337
Van91I	CCANNNNNTGG	1 cut(s)	1192
Vha464I	CTTAAG	1 cut(s)	863
VpaK11BI	GGWCC	3 cut(s)	934, 1643, 1768
XapI	RAATTY	3 cut(s)	459, 615, 1129
XceI	RCATGY	2 cut(s)	1585, 1660
XcmI	CCANNNNNNNNNTGG	1 cut(s)	340
XmaJI	CCTAGG	1 cut(s)	1501
XmnI	GAANNNNTTC	2 cut(s)	1286, 1520
XspI	CTAG	3 cut(s)	642, 1083, 1502
ZraI	GACGTC	1 cut(s)	1614

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.