Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Forward (+)

28932908 .. 28935278

Gene Structure 2371 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1210700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2196 bp

ATGGCGAACAGGGAAGATGTAGATAGGAGATCGAAGAAGCGAATCGTCATCATCAGCCTATCTGCATTTTTATTGATGGCAATGGTGATGGCGGTGGCAGCTGGTTTCATCATGGAAAAGGGTTTCTACAATAGTCCAGTCAGTGGCAGCAAGGGTAGCTCCCATGACAAGTCAGCCTCAATGAAAGCAATCAAAACCATTTGCCAGCCAACCGATTACAAGCAAGAATGTCTCGACAGCCTCAGCTACGCCTCCGGAAACACCACCACCGATCCAATAGAGCTCATTGAATACGGCTTCAAGGTTGCAATGAAGTACGTAACCGATGCTGCCAAAAAATCCGACTTCTTGCGAAAGCTCGAGAAGGATCCCCGAACCTCCAATGCCCTCGACGCGTGCGAAGAGCTTATGGATTTGGCGCTTAGTGAGCTCAGGCAGTCGCTTGTGCGACTAGGGTACATCGATGATTTTACAAAGTTTAATGAAATGTTGGTGGATTTGAAGGTTTGGCTTAGTGCTACAATCACTTATCAAGAAAGCTGCTTGGATGCTTTTGAGAATACTACTAGCTCTACTGATGCAGCTGAGGAGATGAAGAGTGTGTTGAATACCTCTATGCATTTGAGTAGCAATGCCCTTGCCATGGTATCTCAAGTATTTTCAGCCTTCACTGACTTGAACAACCCTGATTCTAGCCACCGCCGCCTCCTCCAATTTGATGCATTCCCTGTTATTGGTCACAGTGAATTTGAAGACCCCAAGTGGTATGAAGGTGGAGTCAGGAAACTCCGACGCCTCCTCCAATTTGATGCCTTCCCTGTTATTGGTCACAGTGAATTCGAAGACCCTAAGTGGTATGAAGGTGGAATCAAGAAACTCCGACGCCTCCTCCAATTTGATGCCTTCCCAGTTATTGGTCACGGGGAATTCGAAGATCCCAAGTGGTATGGAGGTGAAGGCAAGAAACTCCGCCGCCTCCTCCAATTTGATGCCTTCCCTGTTATTGGTCACGGGGAATTCGAAGATCCGAAGTGGTACGGAGGTGAAGGCAAGAAACTCCGCCGCCTCCTCCAATTCGATGTCTTCCCCGTCTTCTCCGTTATTGGTCATGGGGAATTCGAAGATCCCAAGTGGTATGGAGGTGAAGGCAGAGAACTTCTCACCACCAGTACCAGTTCTGCTCCGAAAATTAATAAGCCCGACATCATTGTGGCAAAGGATGGAAGTGGAAGCTACAAGACCATCACTGAGGCGCTAGACCATGTCCCCAAGTATGGCAATGAAACCTTTATCATCTACATCAAGGAAGGAGTATATAATGAGCATATCCTGGTTACCAGGAGCATGACGAGCGTGATGATGATCGGAGATGGCGCAAATAAGACTAGGATCACCGGAAACAAGAACTTTGCAGATGGCACGCCCACCTACCATACTGCCACCGTTGCTATTCAGGGAGATCATTTCATGGCAAGGGATATAGGGTTTGACAACTCTGCTGGTCCGGAGAAACATCAGGCTGTTGCATTGAGGGTGAGTGCTGACGAGGCAATCTTCTACAGGTGTTCAATGGACGGGTACCAAGACACCCTTTACACACACGCACAACGCCAATTCTACTGCGACTGCACCATCTCCGGCACCATAGATTTTGTTTTTGGTGATGCAACTGCAGTCTTCCAAAACTGCACTTTCTTGATCCGCAAGCCATTGCCAAACCAGTCTTGCATTGTGACAGCTCAAGGCAGGAAAGAACGGCGCCAGCCATCTGCAATCATCATTCAAAACAGCACAATCACTGCCGAACCTGATTACTTCCCTGTCAAGGATGAGAACAAGGCATATCTTGGTCGTCCATGGAAGGAGTATTCAAGAACAATCATCATGGACTCATACATTGATGACGTGATCCAACCGGAAGGTTGGTTACCTTGGGAGGGTGAGTGGGGGCTAAAGACATGCTTCTACGCAGAGTCAGGCAACACAGGCCCTGCTGCTAACAAGGCAAGGCGCGTGACATGGCGCGGGATCAAGCAGATTACTGAAAATCATACTGCAGATTTCACACCCGGTAGGTTCTTCAGGGGTGATAAGTGGATTAGGCTCTCAGGTGTTCCTTATATCCCCGGCCTGACCACTAGCAACAAATCGAAAACTGCAACTACAAGGACTCCATCCTTCTCGAAAGGCACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

732

Amino Acids

82.23

Weight (kDa)

6.36

Isoelectric Point (pI)

42.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	62 - 215	7.1e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	401 - 697	3.8e-136	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1578
AccB1I	GGYRCC	4 cut(s)	1578, 1640, 1758, 2189
AccB7I	CCANNNNNTGG	2 cut(s)	143, 914
AccII	CGCG	3 cut(s)	395, 2013, 2025
AccIII	TCCGGA	2 cut(s)	254, 1504
AciI	CCGC	9 cut(s)	92, 700, 703, 970, 973, 1060, 1063, 1702, 2025
AcsI	RAATTY	5 cut(s)	746, 836, 926, 1016, 1115
AcuI	CTGAAG	1 cut(s)	2065
AcyI	GRCGYC	3 cut(s)	793, 883, 1759
AfaI	GTAC	5 cut(s)	317, 458, 1037, 1171, 1580
AfiI	CCNNNNNNNGG	9 cut(s)	143, 643, 734, 824, 914, 1004, 1274, 1825, 1937
AflIII	ACRYGT	1 cut(s)	393
AgsI	TTSAA	9 cut(s)	290, 301, 502, 607, 679, 752, 1569, 1784, 1872
AjiI	CACGTC	1 cut(s)	1906
AjnI	CCWGG	2 cut(s)	1329, 1337
Alw21I	GWGCWC	2 cut(s)	285, 432
Alw26I	GTCTC	1 cut(s)	236
AlwNI	CAGNNNCTG	1 cut(s)	1991
Ama87I	CYCGRG	1 cut(s)	359
Aor13HI	TCCGGA	2 cut(s)	254, 1504
AoxI	GGCC	2 cut(s)	1987, 2128
ApeKI	GCWGC	6 cut(s)	98, 147, 329, 540, 581, 1994
ApoI	RAATTY	5 cut(s)	746, 836, 926, 1016, 1115
AseI	ATTAAT	1 cut(s)	1191
Asp718I	GGTACC	1 cut(s)	1578
AspLEI	GCGC	6 cut(s)	421, 1255, 1376, 1761, 2013, 2025
AspS9I	GGNCC	2 cut(s)	1502, 1988
AsuC2I	CCSGG	2 cut(s)	2070, 2127
AsuII	TTCGAA	4 cut(s)	840, 930, 1020, 1119
AvaI	CYCGRG	1 cut(s)	359
AvaII	GGWCC	1 cut(s)	1502
BaeI	ACNNNNGTAYC	2 cut(s)	1027, 1060
BamHI	GGATCC	1 cut(s)	367
BanI	GGYRCC	4 cut(s)	1578, 1640, 1758, 2189
BanII	GRGCYC	2 cut(s)	285, 432
BarI	GAAGNNNNNNTAC	2 cut(s)	1911, 1943
BbsI	GAAGAC	5 cut(s)	759, 849, 1075, 1084, 1669
Bbv12I	GWGCWC	2 cut(s)	285, 432
BbvCI	CCTCAGC	2 cut(s)	242, 585
BbvI	GCAGC	6 cut(s)	110, 159, 316, 527, 593, 1981
BccI	CCATC	9 cut(s)	70, 82, 1214, 1250, 1364, 1409, 1640, 1774, 2182
BceAI	ACGGC	2 cut(s)	310, 1772
BciT130I	CCWGG	2 cut(s)	1331, 1339
BcnI	CCSGG	2 cut(s)	2070, 2127
BcoDI	GTCTC	1 cut(s)	236
BfaI	CTAG	6 cut(s)	452, 567, 693, 1256, 1386, 2139
BfmI	CTRYAG	3 cut(s)	1558, 1671, 2055
BfoI	RGCGCY	3 cut(s)	422, 1256, 1762
BisI	GCNGC	9 cut(s)	99, 148, 330, 541, 582, 703, 973, 1063, 1995
BlsI	GCNGC	9 cut(s)	100, 149, 331, 542, 583, 704, 974, 1064, 1996
Bme1390I	CCNGG	4 cut(s)	1331, 1339, 2070, 2127
Bme18I	GGWCC	1 cut(s)	1502
BmeT110I	CYCGRG	1 cut(s)	359
BmgBI	CACGTC	1 cut(s)	1906
BmgT120I	GGNCC	2 cut(s)	1502, 1988
BmiI	GGNNCC	5 cut(s)	369, 1580, 1642, 1760, 2191
BmrFI	CCNGG	4 cut(s)	1331, 1339, 2070, 2127
BmrI	ACTGGG	1 cut(s)	902
BmsI	GCATC	8 cut(s)	316, 538, 568, 709, 799, 889, 979, 1654
BmuI	ACTGGG	1 cut(s)	902
BpiI	GAAGAC	5 cut(s)	759, 849, 1075, 1084, 1669
BplI	GAGNNNNNCTC	2 cut(s)	1143, 1175
Bpu10I	CCTNAGC	3 cut(s)	242, 431, 585
Bpu14I	TTCGAA	4 cut(s)	840, 930, 1020, 1119
BpuEI	CTTGAG	2 cut(s)	636, 1725
BpuMI	CCSGG	2 cut(s)	2070, 2127
Bsa29I	ATCGAT	1 cut(s)	462
BsaAI	YACGTR	1 cut(s)	319
BsaBI	GATNNNNATC	1 cut(s)	1361
BsaHI	GRCGYC	3 cut(s)	793, 883, 1759
BsaJI	CCNNGG	4 cut(s)	642, 1856, 1931, 2125
BsaWI	WCCGGW	4 cut(s)	254, 1394, 1504, 1915
BsaXI	ACNNNNNCTCC	8 cut(s)	783, 813, 873, 903, 1928, 1958, 2155, 2185
Bsc4I	CCNNNNNNNGG	9 cut(s)	143, 643, 734, 824, 914, 1004, 1274, 1825, 1937
Bse1I	ACTGG	5 cut(s)	137, 908, 1167, 1173, 1720
Bse3DI	GCAATG	5 cut(s)	87, 315, 637, 1285, 1709
Bse8I	GATNNNNATC	1 cut(s)	1361
BseAI	TCCGGA	2 cut(s)	254, 1504
BseBI	CCWGG	2 cut(s)	1331, 1339
BseCI	ATCGAT	1 cut(s)	462
BseDI	CCNNGG	4 cut(s)	642, 1856, 1931, 2125
BseGI	GGATG	4 cut(s)	553, 1225, 1834, 2174
BseJI	GATNNNNATC	1 cut(s)	1361
BseLI	CCNNNNNNNGG	9 cut(s)	143, 643, 734, 824, 914, 1004, 1274, 1825, 1937
BseMI	GCAATG	5 cut(s)	87, 315, 637, 1285, 1709
BseMII	CTCAG	5 cut(s)	256, 445, 576, 1239, 2121
BseNI	ACTGG	5 cut(s)	137, 908, 1167, 1173, 1720
BseRI	GAGGAG	6 cut(s)	602, 698, 788, 878, 968, 1058
BseXI	GCAGC	6 cut(s)	110, 159, 316, 527, 593, 1981
BsgI	GTGCAG	2 cut(s)	1612, 1672
Bsh1236I	CGCG	3 cut(s)	395, 2013, 2025
BshFI	GGCC	2 cut(s)	1989, 2130
BshNI	GGYRCC	4 cut(s)	1578, 1640, 1758, 2189
BshVI	ATCGAT	1 cut(s)	462
BsiHKAI	GWGCWC	2 cut(s)	285, 432
BsiHKCI	CYCGRG	1 cut(s)	359
BsiSI	CCGG	7 cut(s)	255, 1395, 1505, 1638, 1916, 2070, 2127
BslFI	GGGAC	1 cut(s)	1250
BslI	CCNNNNNNNGG	9 cut(s)	143, 643, 734, 824, 914, 1004, 1274, 1825, 1937
BsmAI	GTCTC	1 cut(s)	236
BsmFI	GGGAC	1 cut(s)	1250
BsmI	GAATGC	1 cut(s)	722
BsnI	GGCC	2 cut(s)	1989, 2130
BsoBI	CYCGRG	1 cut(s)	359
Bsp119I	TTCGAA	4 cut(s)	840, 930, 1020, 1119
Bsp1286I	GDGCHC	2 cut(s)	285, 432
Bsp13I	TCCGGA	2 cut(s)	254, 1504
Bsp19I	CCATGG	2 cut(s)	642, 1856
BspACI	CCGC	9 cut(s)	92, 700, 703, 970, 973, 1060, 1063, 1702, 2025
BspANI	GGCC	2 cut(s)	1989, 2130
BspCNI	CTCAG	5 cut(s)	255, 444, 577, 1240, 2120
BspDI	ATCGAT	1 cut(s)	462
BspEI	TCCGGA	2 cut(s)	254, 1504
BspFNI	CGCG	3 cut(s)	395, 2013, 2025
BspLI	GGNNCC	5 cut(s)	369, 1580, 1642, 1760, 2191
BspMAI	CTGCAG	2 cut(s)	1675, 2059
BspQI	GCTCTTC	1 cut(s)	396
BspT104I	TTCGAA	4 cut(s)	840, 930, 1020, 1119
BspT107I	GGYRCC	4 cut(s)	1578, 1640, 1758, 2189
BsrDI	GCAATG	5 cut(s)	87, 315, 637, 1285, 1709
BsrI	ACTGG	5 cut(s)	137, 908, 1167, 1173, 1720
BssECI	CCNNGG	4 cut(s)	642, 1856, 1931, 2125
BssNI	GRCGYC	3 cut(s)	793, 883, 1759
BssT1I	CCWWGG	3 cut(s)	642, 1856, 1931
Bst2UI	CCWGG	2 cut(s)	1331, 1339
Bst4CI	ACNGT	3 cut(s)	743, 833, 1444
Bst6I	CTCTTC	2 cut(s)	396, 590
BstACI	GRCGYC	3 cut(s)	793, 883, 1759
BstBAI	YACGTR	1 cut(s)	319
BstBI	TTCGAA	4 cut(s)	840, 930, 1020, 1119
BstC8I	GCNNGC	5 cut(s)	206, 397, 1421, 1706, 1763
BstDEI	CTNAG	8 cut(s)	242, 422, 431, 512, 585, 849, 1248, 2107
BstDSI	CCRYGG	2 cut(s)	642, 1856
BstEII	GGTNACC	2 cut(s)	1333, 1926
BstF5I	GGATG	4 cut(s)	553, 1225, 1834, 2174
BstFNI	CGCG	3 cut(s)	395, 2013, 2025
BstH2I	RGCGCY	3 cut(s)	422, 1256, 1762
BstHHI	GCGC	6 cut(s)	421, 1255, 1376, 1761, 2013, 2025
BstMAI	GTCTC	1 cut(s)	236
BstNI	CCWGG	2 cut(s)	1331, 1339
BstNSI	RCATGY	1 cut(s)	1962
BstPI	GGTNACC	2 cut(s)	1333, 1926
BstSCI	CCNGG	4 cut(s)	1329, 1337, 2068, 2125
BstSFI	CTRYAG	3 cut(s)	1558, 1671, 2055
BstSNI	TACGTA	1 cut(s)	319
BstUI	CGCG	3 cut(s)	395, 2013, 2025
BstV1I	GCAGC	6 cut(s)	110, 159, 316, 527, 593, 1981
BstV2I	GAAGAC	5 cut(s)	759, 849, 1075, 1084, 1669
BstX2I	RGATCY	4 cut(s)	367, 934, 1024, 1123
BstYI	RGATCY	4 cut(s)	367, 934, 1024, 1123
Bsu15I	ATCGAT	1 cut(s)	462
BsuRI	GGCC	2 cut(s)	1989, 2130
BsuTUI	ATCGAT	1 cut(s)	462
BtgI	CCRYGG	2 cut(s)	642, 1856
BtrI	CACGTC	1 cut(s)	1906
BtsCI	GGATG	4 cut(s)	553, 1225, 1834, 2174
BtsI	GCAGTG	1 cut(s)	1797
BtsIMutI	CAGTG	6 cut(s)	148, 669, 748, 838, 1245, 1797
Cac8I	GCNNGC	5 cut(s)	206, 397, 1421, 1706, 1763
CaiI	CAGNNNCTG	1 cut(s)	1991
CfoI	GCGC	6 cut(s)	421, 1255, 1376, 1761, 2013, 2025
Cfr13I	GGNCC	2 cut(s)	1502, 1988
ClaI	ATCGAT	1 cut(s)	462
CseI	GACGC	3 cut(s)	401, 801, 891
Csp6I	GTAC	5 cut(s)	316, 457, 1036, 1170, 1579
CviQI	GTAC	5 cut(s)	316, 457, 1036, 1170, 1579
DdeI	CTNAG	8 cut(s)	242, 422, 431, 512, 585, 849, 1248, 2107
DinI	GGCGCC	1 cut(s)	1760
Eam1104I	CTCTTC	2 cut(s)	396, 590
EarI	CTCTTC	2 cut(s)	396, 590
EciI	GGCGGA	2 cut(s)	959, 1049
Ecl136II	GAGCTC	2 cut(s)	283, 430
Eco105I	TACGTA	1 cut(s)	319
Eco130I	CCWWGG	3 cut(s)	642, 1856, 1931
Eco24I	GRGCYC	2 cut(s)	285, 432
Eco47I	GGWCC	1 cut(s)	1502
Eco53kI	GAGCTC	2 cut(s)	283, 430
Eco57I	CTGAAG	1 cut(s)	2065
Eco88I	CYCGRG	1 cut(s)	359
Eco91I	GGTNACC	2 cut(s)	1333, 1926
EcoICRI	GAGCTC	2 cut(s)	283, 430
EcoO109I	RGGNCCY	1 cut(s)	1988
EcoO65I	GGTNACC	2 cut(s)	1333, 1926
EcoRI	GAATTC	4 cut(s)	836, 926, 1016, 1115
EcoRII	CCWGG	2 cut(s)	1329, 1337
EcoT14I	CCWWGG	3 cut(s)	642, 1856, 1931
EcoT22I	ATGCAT	2 cut(s)	621, 724
EcoT38I	GRGCYC	2 cut(s)	285, 432
EgeI	GGCGCC	1 cut(s)	1760
EheI	GGCGCC	1 cut(s)	1760
ErhI	CCWWGG	3 cut(s)	642, 1856, 1931
FalI	AAGNNNNNCTT	4 cut(s)	1575, 1607, 1946, 1978
FaqI	GGGAC	1 cut(s)	1250
FauI	CCCGC	1 cut(s)	2018
Fnu4HI	GCNGC	9 cut(s)	99, 148, 330, 541, 582, 703, 973, 1063, 1995
FokI	GGATG	4 cut(s)	560, 1232, 1841, 2161
FriOI	GRGCYC	2 cut(s)	285, 432
Fsp4HI	GCNGC	9 cut(s)	99, 148, 330, 541, 582, 703, 973, 1063, 1995
FspBI	CTAG	6 cut(s)	452, 567, 693, 1256, 1386, 2139
GlaI	GCGC	6 cut(s)	420, 1254, 1375, 1760, 2012, 2024
GluI	GCNGC	9 cut(s)	99, 148, 330, 541, 582, 703, 973, 1063, 1995
HaeII	RGCGCY	3 cut(s)	422, 1256, 1762
HaeIII	GGCC	2 cut(s)	1989, 2130
HapII	CCGG	7 cut(s)	255, 1395, 1505, 1638, 1916, 2070, 2127
HgaI	GACGC	3 cut(s)	401, 801, 891
HhaI	GCGC	6 cut(s)	421, 1255, 1376, 1761, 2013, 2025
Hin1I	GRCGYC	3 cut(s)	793, 883, 1759
Hin6I	GCGC	6 cut(s)	419, 1253, 1374, 1759, 2011, 2023
HinP1I	GCGC	6 cut(s)	419, 1253, 1374, 1759, 2011, 2023
HinfI	GANTC	7 cut(s)	42, 689, 777, 867, 1889, 1973, 2170
HpaII	CCGG	7 cut(s)	255, 1395, 1505, 1638, 1916, 2070, 2127
Hpy188I	TCNGA	6 cut(s)	343, 791, 881, 1029, 1185, 1367
Hpy99I	CGWCG	3 cut(s)	395, 795, 885
HpyCH4III	ACNGT	3 cut(s)	743, 833, 1444
HpyCH4IV	ACGT	2 cut(s)	318, 1905
HpyF3I	CTNAG	8 cut(s)	242, 422, 431, 512, 585, 849, 1248, 2107
HpySE526I	ACGT	2 cut(s)	318, 1905
Hsp92I	GRCGYC	3 cut(s)	793, 883, 1759
HspAI	GCGC	6 cut(s)	419, 1253, 1374, 1759, 2011, 2023
KasI	GGCGCC	1 cut(s)	1758
Kpn2I	TCCGGA	2 cut(s)	254, 1504
KpnI	GGTACC	1 cut(s)	1582
LguI	GCTCTTC	1 cut(s)	396
LmnI	GCTCC	3 cut(s)	164, 1186, 1341
Lsp1109I	GCAGC	6 cut(s)	110, 159, 316, 527, 593, 1981
LweI	GCATC	8 cut(s)	316, 538, 568, 709, 799, 889, 979, 1654
MaeI	CTAG	6 cut(s)	452, 567, 693, 1256, 1386, 2139
MaeII	ACGT	2 cut(s)	318, 1905
MaeIII	GTNAC	9 cut(s)	319, 737, 827, 917, 1007, 1333, 1732, 1926, 2014
MflI	RGATCY	4 cut(s)	367, 934, 1024, 1123
MhlI	GDGCHC	2 cut(s)	285, 432
MluI	ACGCGT	1 cut(s)	393
Mly113I	GGCGCC	1 cut(s)	1759
MlyI	GAGTC	4 cut(s)	786, 1883, 1982, 2164
MmeI	TCCRAC	4 cut(s)	366, 814, 904, 1936
Mph1103I	ATGCAT	2 cut(s)	621, 724
MroI	TCCGGA	2 cut(s)	254, 1504
MseI	TTAA	2 cut(s)	480, 1191
MslI	CAYNNNNRTG	1 cut(s)	1208
MspA1I	CMGCKG	2 cut(s)	101, 584
MspI	CCGG	7 cut(s)	255, 1395, 1505, 1638, 1916, 2070, 2127
MspR9I	CCNGG	4 cut(s)	1331, 1339, 2070, 2127
Mva1269I	GAATGC	1 cut(s)	722
MvaI	CCWGG	2 cut(s)	1331, 1339
MvnI	CGCG	3 cut(s)	395, 2013, 2025
NarI	GGCGCC	1 cut(s)	1759
NciI	CCSGG	2 cut(s)	2070, 2127
NcoI	CCATGG	2 cut(s)	642, 1856
NlaIV	GGNNCC	5 cut(s)	369, 1580, 1642, 1760, 2191
NmuCI	GTSAC	6 cut(s)	737, 827, 917, 1007, 1732, 2014
NsiI	ATGCAT	2 cut(s)	621, 724
NspI	RCATGY	1 cut(s)	1962
NspV	TTCGAA	4 cut(s)	840, 930, 1020, 1119
PaeR7I	CTCGAG	1 cut(s)	359
PciSI	GCTCTTC	1 cut(s)	396
PcsI	WCGNNNNNNNCGW	3 cut(s)	396, 927, 1017
PctI	GAATGC	1 cut(s)	722
PfeI	GAWTC	3 cut(s)	42, 689, 867
PflFI	GACNNNGTC	1 cut(s)	1262
PflMI	CCANNNNNTGG	2 cut(s)	143, 914
PkrI	GCNGC	9 cut(s)	100, 149, 331, 542, 583, 704, 974, 1064, 1996
PleI	GAGTC	4 cut(s)	785, 1883, 1981, 2164
PluTI	GGCGCC	1 cut(s)	1762
PpsI	GAGTC	4 cut(s)	785, 1883, 1981, 2164
Ppu21I	YACGTR	1 cut(s)	319
PshBI	ATTAAT	1 cut(s)	1191
Psp124BI	GAGCTC	2 cut(s)	285, 432
Psp6I	CCWGG	2 cut(s)	1329, 1337
PspEI	GGTNACC	2 cut(s)	1333, 1926
PspGI	CCWGG	2 cut(s)	1329, 1337
PspN4I	GGNNCC	5 cut(s)	369, 1580, 1642, 1760, 2191
PspPI	GGNCC	2 cut(s)	1502, 1988
PstI	CTGCAG	2 cut(s)	1675, 2059
PstNI	CAGNNNCTG	1 cut(s)	1991
PsuI	RGATCY	4 cut(s)	367, 934, 1024, 1123
PsyI	GACNNNGTC	1 cut(s)	1262
PvuII	CAGCTG	2 cut(s)	101, 584
RsaI	GTAC	5 cut(s)	317, 458, 1037, 1171, 1580
RsaNI	GTAC	5 cut(s)	316, 457, 1036, 1170, 1579
RseI	CAYNNNNRTG	1 cut(s)	1208
SacI	GAGCTC	2 cut(s)	285, 432
SapI	GCTCTTC	1 cut(s)	396
SaqAI	TTAA	2 cut(s)	480, 1191
SatI	GCNGC	9 cut(s)	99, 148, 330, 541, 582, 703, 973, 1063, 1995
Sau96I	GGNCC	2 cut(s)	1502, 1988
SchI	GAGTC	4 cut(s)	786, 1883, 1982, 2164
ScrFI	CCNGG	4 cut(s)	1331, 1339, 2070, 2127
SduI	GDGCHC	2 cut(s)	285, 432
SfaNI	GCATC	8 cut(s)	316, 538, 568, 709, 799, 889, 979, 1654
SfcI	CTRYAG	3 cut(s)	1558, 1671, 2055
SfoI	GGCGCC	1 cut(s)	1760
Sfr274I	CTCGAG	1 cut(s)	359
SfuI	TTCGAA	4 cut(s)	840, 930, 1020, 1119
SinI	GGWCC	1 cut(s)	1502
SlaI	CTCGAG	1 cut(s)	359
SmiMI	CAYNNNNRTG	1 cut(s)	1208
SmlI	CTYRAG	3 cut(s)	359, 651, 1740
SmoI	CTYRAG	3 cut(s)	359, 651, 1740
SnaBI	TACGTA	1 cut(s)	319
SsiI	CCGC	9 cut(s)	92, 700, 703, 970, 973, 1060, 1063, 1702, 2025
SspDI	GGCGCC	1 cut(s)	1758
SspMI	CTAG	6 cut(s)	452, 567, 693, 1256, 1386, 2139
SstI	GAGCTC	2 cut(s)	285, 432
StyD4I	CCNGG	4 cut(s)	1329, 1337, 2068, 2125
StyI	CCWWGG	3 cut(s)	642, 1856, 1931
TaaI	ACNGT	3 cut(s)	743, 833, 1444
TaiI	ACGT	2 cut(s)	321, 1908
TauI	GCSGC	3 cut(s)	705, 975, 1065
TfiI	GAWTC	3 cut(s)	42, 689, 867
Tru1I	TTAA	2 cut(s)	480, 1191
Tru9I	TTAA	2 cut(s)	480, 1191
TscAI	CASTG	6 cut(s)	148, 676, 748, 838, 1252, 1804
TseFI	GTSAC	6 cut(s)	737, 827, 917, 1007, 1732, 2014
TseI	GCWGC	6 cut(s)	98, 147, 329, 540, 581, 1994
Tsp45I	GTSAC	6 cut(s)	737, 827, 917, 1007, 1732, 2014
TspDTI	ATGAA	9 cut(s)	97, 197, 326, 498, 608, 783, 873, 1296, 1456
TspGWI	ACGGA	2 cut(s)	1053, 1087
TspRI	CASTG	6 cut(s)	148, 676, 748, 838, 1252, 1804
Tth111I	GACNNNGTC	1 cut(s)	1262
Van91I	CCANNNNNTGG	2 cut(s)	143, 914
VpaK11BI	GGWCC	1 cut(s)	1502
VspI	ATTAAT	1 cut(s)	1191
XapI	RAATTY	5 cut(s)	746, 836, 926, 1016, 1115
XceI	RCATGY	1 cut(s)	1962
XhoI	CTCGAG	1 cut(s)	359
XspI	CTAG	6 cut(s)	452, 567, 693, 1256, 1386, 2139
Zsp2I	ATGCAT	2 cut(s)	621, 724

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.