Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Forward (+)

28579275 .. 28581504

Gene Structure 2230 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1198100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2097 bp

ATGGCGTACGGAGACGACGCAGATAGGAGATCGAGAAAGCGAGTCGTCATTATCAGCGTCTCGGCGTTTTTATTGGTGGCAATGGTGGTGGCGGTGACGGTTGGTTTTGCCATGGAAAAGAGTTTCTACAATAGTCCATATAGTGGCAACAAAGGCAGGTCCCATGACAAGCCAGCCTCAATGAAAGCAATCAAAGCCATTTGCCAACCAACCGATTACAAGCAAGAATGTCTCGACAGCCTCAGCTACGCCTCCGGAAACACCACCACGGATCCAGTACAGCTCATTGGATACGGCTTCAAGGTTGCAATGAAGTACGTTTACGATGCTGCCAAAAAATCCGACTTCCTGCGAAAGCTCGAGAAGGATCCCCGAACCTCCAATGCCCTCGACACATGCGAAGAGCTCATGGAATTGGCGCTTGGTGAGCTCAGGCAATCGCTTGTGCGATTGGGGTTCATCGATGATCTTACGAAGTTTAATGACATGTTGATGGATTTGAAGGTTTGGCTGAGCGCTACAATCACTTACCAAGAAAGCTGCTTGGATGCTTTTGAGAACACAACTAGTTCTACCGATGCAGCGGAGGAGATGAAGAATGTGTTGAAAACCTCCATGCATTTGAGCAGCAATGCCCTTGCCATGGTTTCTCAAGTCTTTTCAGCCTTCACTGACTTGAACAACCCTGACCCTAGCCACCGCCGCCTCCTCCAATTTGATGCCTTCCCTGTTATTGGTCACAGTGAATTCGAAGACCCCAAGTGGTATGGAGGTGAAGGCAAGAAACTCCGCCGCCTCCTCCAATTTGACACCTTCCCTGTTATTGGTCATGGGGAATTCGAAGATCCCAAGTGGTATGGAGGTGAAGGCAGGAAACTCCGCCGCCTCCTCCAATTTGATGCCTTCCCAGTTATGGGTCACGGGGAATTTGACGATCCCAAGTGGTATGGAGGTGAAGGCAGGAAACTCCGCCGGCTCCTCCAATTTGATGCCTTCCCAGTTATTGGTCATGGGGAATTCGAAGATCCAAAATGGTATGGAGGTGAAGGCAGGAAACTTCTCACAACCAGTACCACTTCTGCTCCGAAAATTAATAAGCCCGATATCATTGTGGCAAAGGATGGAAGTGGAAGCTACAAGACCATCACTGAGGCACTAAAACATGTCCCCAAGTATGGCAATGAGACCTTTATCATCTACATCAAGGAAGGTATATATAATGAGCATATCCTGGTTACTAGGAGCATGACGAATGTGATGATGATCGGAGATGGTGCGAATAAGACCAGGATAACCGGAAACAAGAACTTTGTAGATGGCACGACCACCTACCATACTGCCACTGTTGCTATTCAGGGAGACCATTTCATGGTAATGGACATAGGGTTTGAGAACTCTGCTGGTCCGGAGAAACATCAGGCTGTTGCATTGAGGGTAAGCGCTGACAAGGCAATCTTCTACAGGTGCTCGATGGATGGTTACCAAGACACCCTCTACACTCATGCTCAACGCCAATTCTACCGCGACTGCACCATCTCCGGCACCATAGATTTTGTGTTCGGAGATGCTACTGCAATCTTCCAAAACTGCACTTTCTTGATCCGCAAGCCATTGCCAAACCAGTCTTGCATTGTGACAGCTCAAGGCAGGAAAGAACGGCGCCAACCATCTGCAATCATCATTCAAAACAGCACAATCACCGCCGATCCTGATTACTTCCCTGTCAAGGATGAGAACAAGGCATATCTTGGCCGTCCATGGAAGGAATACTCGAGGACAATCATCATGGACTCGTACATTGATGACGTGATTCAACCGGAAGGTTGGTTGCCATGGGAGGGTGAGTGGGGGCTCAAGACATGCTTCTACGCAGAGTTAGGCAACACAGGCCCTGCTGCTAACAAGGCAAGGCGCGTGACATGGCGCGGGATCAAGAAGATTACTGAAAATCATGCTGCAGATTTCACACCCGGTAGGTTCTTCAGGGGCGATAAGTGGATTAGGCTCTCCGGTGTGCCCTATATCCCCGGCCTGACCACTAGGAACCAACCGAAAACTGCAACTATAAGTACTCAATCCGTCTTGAAAGGCGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

699

Amino Acids

78.53

Weight (kDa)

8.32

Isoelectric Point (pI)

36.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	62 - 215	3.3e-28	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	368 - 664	3.5e-138	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	147
AccB1I	GGYRCC	3 cut(s)	1541, 1659, 2090
AccB7I	CCANNNNNTGG	3 cut(s)	143, 1004, 1369
AccII	CGCG	3 cut(s)	1524, 1914, 1926
AccIII	TCCGGA	2 cut(s)	254, 1405
AcoI	YGGCCR	1 cut(s)	1750
AcsI	RAATTY	4 cut(s)	746, 836, 926, 1016
AcuI	CTGAAG	1 cut(s)	1966
AcyI	GRCGYC	2 cut(s)	1660, 2091
AfaI	GTAC	6 cut(s)	8, 279, 317, 1072, 1796, 2071
AfeI	AGCGCT	2 cut(s)	517, 1441
AflIII	ACRYGT	2 cut(s)	486, 1162
AgsI	TTSAA	7 cut(s)	301, 502, 607, 679, 1685, 1814, 2086
AhlI	ACTAGT	1 cut(s)	566
AjiI	CACGTC	1 cut(s)	1807
AjnI	CCWGG	2 cut(s)	1230, 1286
AjuI	GAANNNNNNNTTGG	2 cut(s)	405, 437
AluBI	AGCT	8 cut(s)	246, 283, 358, 406, 430, 540, 1134, 1640
AluI	AGCT	8 cut(s)	246, 283, 358, 406, 430, 540, 1134, 1640
Alw21I	GWGCWC	3 cut(s)	408, 432, 1469
Alw26I	GTCTC	5 cut(s)	6, 64, 236, 1178, 1353
AlwNI	CAGNNNCTG	1 cut(s)	1892
Ama87I	CYCGRG	2 cut(s)	359, 1771
Aor13HI	TCCGGA	2 cut(s)	254, 1405
Aor51HI	AGCGCT	2 cut(s)	517, 1441
AoxI	GGCC	3 cut(s)	1750, 1888, 2029
ApeKI	GCWGC	6 cut(s)	329, 540, 581, 627, 1895, 1955
ApoI	RAATTY	4 cut(s)	746, 836, 926, 1016
ArsI	GACNNNNNNTTYG	2 cut(s)	1370, 1402
AseI	ATTAAT	1 cut(s)	1092
AspLEI	GCGC	7 cut(s)	421, 518, 1442, 1662, 1914, 1926, 2093
AspS9I	GGNCC	3 cut(s)	159, 1403, 1889
AsuC2I	CCSGG	2 cut(s)	1971, 2028
AsuHPI	GGTGA	8 cut(s)	106, 437, 785, 875, 965, 1055, 1690, 1853
AsuII	TTCGAA	3 cut(s)	750, 840, 1020
AvaI	CYCGRG	2 cut(s)	359, 1771
AvaII	GGWCC	2 cut(s)	159, 1403
BaeGI	GKGCMC	1 cut(s)	2019
BamHI	GGATCC	2 cut(s)	271, 367
BanI	GGYRCC	3 cut(s)	1541, 1659, 2090
BanII	GRGCYC	3 cut(s)	408, 432, 1854
BarI	GAAGNNNNNNTAC	2 cut(s)	305, 337
BbsI	GAAGAC	1 cut(s)	759
Bbv12I	GWGCWC	3 cut(s)	408, 432, 1469
BbvCI	CCTCAGC	1 cut(s)	242
BbvI	GCAGC	6 cut(s)	316, 527, 593, 639, 1882, 1942
BccI	CCATC	9 cut(s)	487, 1115, 1151, 1265, 1310, 1465, 1469, 1541, 1675
BceAI	ACGGC	3 cut(s)	310, 1673, 1737
BciT130I	CCWGG	2 cut(s)	1232, 1288
BciVI	GTATCC	1 cut(s)	284
BcnI	CCSGG	2 cut(s)	1971, 2028
BcoDI	GTCTC	5 cut(s)	6, 64, 236, 1178, 1353
BcuI	ACTAGT	1 cut(s)	566
BfaI	CTAG	4 cut(s)	567, 693, 1239, 2040
BfmI	CTRYAG	2 cut(s)	1459, 1956
BfoI	RGCGCY	5 cut(s)	422, 519, 1443, 1663, 2094
BfuAI	ACCTGC	1 cut(s)	147
BfuI	GTATCC	1 cut(s)	284
BisI	GCNGC	9 cut(s)	330, 541, 582, 628, 703, 793, 883, 1896, 1956
BlpI	GCTNAGC	1 cut(s)	512
BlsI	GCNGC	9 cut(s)	331, 542, 583, 629, 704, 794, 884, 1897, 1957
BmcAI	AGTACT	1 cut(s)	2071
Bme1390I	CCNGG	4 cut(s)	1232, 1288, 1971, 2028
Bme18I	GGWCC	2 cut(s)	159, 1403
BmeT110I	CYCGRG	2 cut(s)	359, 1771
BmgBI	CACGTC	1 cut(s)	1807
BmgT120I	GGNCC	3 cut(s)	159, 1403, 1889
BmiI	GGNNCC	8 cut(s)	161, 273, 369, 977, 1543, 1661, 2045, 2092
BmrFI	CCNGG	4 cut(s)	1232, 1288, 1971, 2028
BmrI	ACTGGG	2 cut(s)	902, 992
BmsI	GCATC	7 cut(s)	316, 538, 568, 709, 889, 979, 1555
BmuI	ACTGGG	2 cut(s)	902, 992
BpiI	GAAGAC	1 cut(s)	759
Bpu10I	CCTNAGC	2 cut(s)	242, 431
Bpu1102I	GCTNAGC	1 cut(s)	512
Bpu14I	TTCGAA	3 cut(s)	750, 840, 1020
BpuEI	CTTGAG	3 cut(s)	636, 1626, 1838
BpuMI	CCSGG	2 cut(s)	1971, 2028
Bsa29I	ATCGAT	1 cut(s)	462
BsaBI	GATNNNNATC	1 cut(s)	1262
BsaHI	GRCGYC	2 cut(s)	1660, 2091
BsaI	GGTCTC	2 cut(s)	1178, 1353
BsaJI	CCNNGG	6 cut(s)	111, 267, 642, 1757, 1832, 2026
BsaWI	WCCGGW	5 cut(s)	254, 1295, 1405, 1816, 2009
BsaXI	ACNNNNNCTCC	4 cut(s)	1066, 1096, 1829, 1859
Bse118I	RCCGGY	1 cut(s)	972
Bse1I	ACTGG	5 cut(s)	275, 908, 998, 1068, 1621
Bse3DI	GCAATG	5 cut(s)	87, 315, 637, 1186, 1610
Bse8I	GATNNNNATC	1 cut(s)	1262
BseAI	TCCGGA	2 cut(s)	254, 1405
BseBI	CCWGG	2 cut(s)	1232, 1288
BseCI	ATCGAT	1 cut(s)	462
BseDI	CCNNGG	6 cut(s)	111, 267, 642, 1757, 1832, 2026
BseGI	GGATG	4 cut(s)	553, 1126, 1480, 1735
BseJI	GATNNNNATC	1 cut(s)	1262
BseMI	GCAATG	5 cut(s)	87, 315, 637, 1186, 1610
BseMII	CTCAG	4 cut(s)	256, 445, 503, 1140
BseNI	ACTGG	5 cut(s)	275, 908, 998, 1068, 1621
BseRI	GAGGAG	5 cut(s)	602, 698, 788, 878, 968
BseSI	GKGCMC	1 cut(s)	2019
BseXI	GCAGC	6 cut(s)	316, 527, 593, 639, 1882, 1942
BsgI	GTGCAG	2 cut(s)	1513, 1573
Bsh1236I	CGCG	3 cut(s)	1524, 1914, 1926
BshFI	GGCC	3 cut(s)	1752, 1890, 2031
BshNI	GGYRCC	3 cut(s)	1541, 1659, 2090
BshVI	ATCGAT	1 cut(s)	462
BsiHKAI	GWGCWC	3 cut(s)	408, 432, 1469
BsiHKCI	CYCGRG	2 cut(s)	359, 1771
BsiSI	CCGG	9 cut(s)	255, 973, 1296, 1406, 1539, 1817, 1971, 2010, 2028
BsiWI	CGTACG	1 cut(s)	6
BslFI	GGGAC	2 cut(s)	145, 1151
BsmAI	GTCTC	5 cut(s)	6, 64, 236, 1178, 1353
BsmBI	CGTCTC	2 cut(s)	6, 64
BsmFI	GGGAC	2 cut(s)	145, 1151
BsnI	GGCC	3 cut(s)	1752, 1890, 2031
Bso31I	GGTCTC	2 cut(s)	1178, 1353
BsoBI	CYCGRG	2 cut(s)	359, 1771
Bsp119I	TTCGAA	3 cut(s)	750, 840, 1020
Bsp1286I	GDGCHC	5 cut(s)	408, 432, 1469, 1854, 2019
Bsp13I	TCCGGA	2 cut(s)	254, 1405
Bsp1720I	GCTNAGC	1 cut(s)	512
Bsp19I	CCATGG	4 cut(s)	111, 642, 1757, 1832
BspANI	GGCC	3 cut(s)	1752, 1890, 2031
BspCNI	CTCAG	4 cut(s)	255, 444, 504, 1141
BspDI	ATCGAT	1 cut(s)	462
BspEI	TCCGGA	2 cut(s)	254, 1405
BspFNI	CGCG	3 cut(s)	1524, 1914, 1926
BspLI	GGNNCC	8 cut(s)	161, 273, 369, 977, 1543, 1661, 2045, 2092
BspMAI	CTGCAG	1 cut(s)	1960
BspMI	ACCTGC	1 cut(s)	147
BspQI	GCTCTTC	1 cut(s)	396
BspT104I	TTCGAA	3 cut(s)	750, 840, 1020
BspT107I	GGYRCC	3 cut(s)	1541, 1659, 2090
BspTNI	GGTCTC	2 cut(s)	1178, 1353
BsrDI	GCAATG	5 cut(s)	87, 315, 637, 1186, 1610
BsrFI	RCCGGY	1 cut(s)	972
BsrI	ACTGG	5 cut(s)	275, 908, 998, 1068, 1621
BssAI	RCCGGY	1 cut(s)	972
BssECI	CCNNGG	6 cut(s)	111, 267, 642, 1757, 1832, 2026
BssNI	GRCGYC	2 cut(s)	1660, 2091
BssT1I	CCWWGG	4 cut(s)	111, 642, 1757, 1832
Bst2UI	CCWGG	2 cut(s)	1232, 1288
Bst4CI	ACNGT	3 cut(s)	100, 743, 1345
Bst6I	CTCTTC	1 cut(s)	396
BstACI	GRCGYC	2 cut(s)	1660, 2091
BstBI	TTCGAA	3 cut(s)	750, 840, 1020
BstC8I	GCNNGC	3 cut(s)	174, 974, 1607
BstDEI	CTNAG	4 cut(s)	242, 431, 512, 1149
BstDSI	CCRYGG	5 cut(s)	111, 267, 642, 1757, 1832
BstEII	GGTNACC	1 cut(s)	1478
BstF5I	GGATG	4 cut(s)	553, 1126, 1480, 1735
BstFNI	CGCG	3 cut(s)	1524, 1914, 1926
BstH2I	RGCGCY	5 cut(s)	422, 519, 1443, 1663, 2094
BstHHI	GCGC	7 cut(s)	421, 518, 1442, 1662, 1914, 1926, 2093
BstMAI	GTCTC	5 cut(s)	6, 64, 236, 1178, 1353
BstMWI	GCNNNNNNNGC	8 cut(s)	153, 194, 427, 702, 1346, 1448, 1887, 1904
BstNI	CCWGG	2 cut(s)	1232, 1288
BstNSI	RCATGY	4 cut(s)	399, 490, 1166, 1863
BstPI	GGTNACC	1 cut(s)	1478
BstSCI	CCNGG	4 cut(s)	1230, 1286, 1969, 2026
BstSFI	CTRYAG	2 cut(s)	1459, 1956
BstSLI	GKGCMC	1 cut(s)	2019
BstUI	CGCG	3 cut(s)	1524, 1914, 1926
BstV1I	GCAGC	6 cut(s)	316, 527, 593, 639, 1882, 1942
BstV2I	GAAGAC	1 cut(s)	759
BstX2I	RGATCY	4 cut(s)	271, 367, 844, 1024
BstYI	RGATCY	4 cut(s)	271, 367, 844, 1024
Bsu15I	ATCGAT	1 cut(s)	462
BsuI	GTATCC	1 cut(s)	284
BsuRI	GGCC	3 cut(s)	1752, 1890, 2031
BsuTUI	ATCGAT	1 cut(s)	462
BtgI	CCRYGG	5 cut(s)	111, 267, 642, 1757, 1832
BtrI	CACGTC	1 cut(s)	1807
BtsCI	GGATG	4 cut(s)	553, 1126, 1480, 1735
BtsIMutI	CAGTG	4 cut(s)	669, 748, 1146, 1341
BveI	ACCTGC	1 cut(s)	147
Cac8I	GCNNGC	3 cut(s)	174, 974, 1607
CaiI	CAGNNNCTG	1 cut(s)	1892
CfoI	GCGC	7 cut(s)	421, 518, 1442, 1662, 1914, 1926, 2093
Cfr10I	RCCGGY	1 cut(s)	972
Cfr13I	GGNCC	3 cut(s)	159, 1403, 1889
ClaI	ATCGAT	1 cut(s)	462
CseI	GACGC	2 cut(s)	26, 46
Csp6I	GTAC	6 cut(s)	7, 278, 316, 1071, 1795, 2070
CspCI	CAANNNNNGTGG	2 cut(s)	69, 104
CviQI	GTAC	6 cut(s)	7, 278, 316, 1071, 1795, 2070
DdeI	CTNAG	4 cut(s)	242, 431, 512, 1149
DinI	GGCGCC	2 cut(s)	1661, 2092
EaeI	YGGCCR	1 cut(s)	1750
Eam1104I	CTCTTC	1 cut(s)	396
EarI	CTCTTC	1 cut(s)	396
EciI	GGCGGA	3 cut(s)	779, 869, 959
Ecl136II	GAGCTC	2 cut(s)	406, 430
Eco130I	CCWWGG	4 cut(s)	111, 642, 1757, 1832
Eco24I	GRGCYC	3 cut(s)	408, 432, 1854
Eco31I	GGTCTC	2 cut(s)	1178, 1353
Eco32I	GATATC	1 cut(s)	1105
Eco47I	GGWCC	2 cut(s)	159, 1403
Eco47III	AGCGCT	2 cut(s)	517, 1441
Eco53kI	GAGCTC	2 cut(s)	406, 430
Eco57I	CTGAAG	1 cut(s)	1966
Eco88I	CYCGRG	2 cut(s)	359, 1771
Eco91I	GGTNACC	1 cut(s)	1478
EcoICRI	GAGCTC	2 cut(s)	406, 430
EcoO109I	RGGNCCY	2 cut(s)	159, 1889
EcoO65I	GGTNACC	1 cut(s)	1478
EcoRI	GAATTC	3 cut(s)	746, 836, 1016
EcoRII	CCWGG	2 cut(s)	1230, 1286
EcoRV	GATATC	1 cut(s)	1105
EcoT14I	CCWWGG	4 cut(s)	111, 642, 1757, 1832
EcoT22I	ATGCAT	1 cut(s)	621
EcoT38I	GRGCYC	3 cut(s)	408, 432, 1854
EgeI	GGCGCC	2 cut(s)	1661, 2092
EheI	GGCGCC	2 cut(s)	1661, 2092
ErhI	CCWWGG	4 cut(s)	111, 642, 1757, 1832
Esp3I	CGTCTC	2 cut(s)	6, 64
FalI	AAGNNNNNCTT	4 cut(s)	1439, 1471, 1847, 1879
FaqI	GGGAC	2 cut(s)	145, 1151
FauI	CCCGC	1 cut(s)	1919
Fnu4HI	GCNGC	9 cut(s)	330, 541, 582, 628, 703, 793, 883, 1896, 1956
FokI	GGATG	4 cut(s)	560, 1133, 1487, 1742
FriOI	GRGCYC	3 cut(s)	408, 432, 1854
Fsp4HI	GCNGC	9 cut(s)	330, 541, 582, 628, 703, 793, 883, 1896, 1956
FspBI	CTAG	4 cut(s)	567, 693, 1239, 2040
GlaI	GCGC	7 cut(s)	420, 517, 1441, 1661, 1913, 1925, 2092
GluI	GCNGC	9 cut(s)	330, 541, 582, 628, 703, 793, 883, 1896, 1956
HaeII	RGCGCY	5 cut(s)	422, 519, 1443, 1663, 2094
HaeIII	GGCC	3 cut(s)	1752, 1890, 2031
HapII	CCGG	9 cut(s)	255, 973, 1296, 1406, 1539, 1817, 1971, 2010, 2028
HgaI	GACGC	2 cut(s)	26, 46
HhaI	GCGC	7 cut(s)	421, 518, 1442, 1662, 1914, 1926, 2093
Hin1I	GRCGYC	2 cut(s)	1660, 2091
Hin6I	GCGC	7 cut(s)	419, 516, 1440, 1660, 1912, 1924, 2091
HinP1I	GCGC	7 cut(s)	419, 516, 1440, 1660, 1912, 1924, 2091
HinfI	GANTC	3 cut(s)	42, 1790, 1810
HpaII	CCGG	9 cut(s)	255, 973, 1296, 1406, 1539, 1817, 1971, 2010, 2028
HphI	GGTGA	8 cut(s)	106, 437, 785, 875, 965, 1055, 1690, 1853
Hpy166II	GTNNAC	1 cut(s)	322
Hpy188I	TCNGA	4 cut(s)	343, 1086, 1268, 1562
Hpy8I	GTNNAC	1 cut(s)	322
Hpy99I	CGWCG	1 cut(s)	20
HpyCH4III	ACNGT	3 cut(s)	100, 743, 1345
HpyCH4IV	ACGT	2 cut(s)	318, 1806
HpyF10VI	GCNNNNNNNGC	8 cut(s)	153, 194, 427, 702, 1346, 1448, 1887, 1904
HpyF3I	CTNAG	4 cut(s)	242, 431, 512, 1149
HpySE526I	ACGT	2 cut(s)	318, 1806
Hsp92I	GRCGYC	2 cut(s)	1660, 2091
HspAI	GCGC	7 cut(s)	419, 516, 1440, 1660, 1912, 1924, 2091
KasI	GGCGCC	2 cut(s)	1659, 2090
Kpn2I	TCCGGA	2 cut(s)	254, 1405
KroI	GCCGGC	1 cut(s)	972
KroNI	GCCGGC	1 cut(s)	974
LguI	GCTCTTC	1 cut(s)	396
LmnI	GCTCC	3 cut(s)	981, 1087, 1242
Lsp1109I	GCAGC	6 cut(s)	316, 527, 593, 639, 1882, 1942
LweI	GCATC	7 cut(s)	316, 538, 568, 709, 889, 979, 1555
MaeI	CTAG	4 cut(s)	567, 693, 1239, 2040
MaeII	ACGT	2 cut(s)	318, 1806
MaeIII	GTNAC	7 cut(s)	94, 737, 917, 1234, 1478, 1633, 1915
MboII	GAAGA	9 cut(s)	413, 607, 764, 854, 1034, 1447, 1570, 1948, 1972
MflI	RGATCY	4 cut(s)	271, 367, 844, 1024
MhlI	GDGCHC	5 cut(s)	408, 432, 1469, 1854, 2019
Mly113I	GGCGCC	2 cut(s)	1660, 2091
MlyI	GAGTC	2 cut(s)	51, 1784
MmeI	TCCRAC	1 cut(s)	366
Mph1103I	ATGCAT	1 cut(s)	621
MroI	TCCGGA	2 cut(s)	254, 1405
MroNI	GCCGGC	1 cut(s)	972
MseI	TTAA	2 cut(s)	480, 1092
MslI	CAYNNNNRTG	2 cut(s)	491, 1373
MspA1I	CMGCKG	1 cut(s)	584
MspI	CCGG	9 cut(s)	255, 973, 1296, 1406, 1539, 1817, 1971, 2010, 2028
MspR9I	CCNGG	4 cut(s)	1232, 1288, 1971, 2028
MvaI	CCWGG	2 cut(s)	1232, 1288
MvnI	CGCG	3 cut(s)	1524, 1914, 1926
MwoI	GCNNNNNNNGC	8 cut(s)	153, 194, 427, 702, 1346, 1448, 1887, 1904
NaeI	GCCGGC	1 cut(s)	974
NarI	GGCGCC	2 cut(s)	1660, 2091
NciI	CCSGG	2 cut(s)	1971, 2028
NcoI	CCATGG	4 cut(s)	111, 642, 1757, 1832
NgoMIV	GCCGGC	1 cut(s)	972
NlaIV	GGNNCC	8 cut(s)	161, 273, 369, 977, 1543, 1661, 2045, 2092
NmeAIII	GCCGAG	1 cut(s)	41
NmuCI	GTSAC	5 cut(s)	94, 737, 917, 1633, 1915
NsiI	ATGCAT	1 cut(s)	621
NspI	RCATGY	4 cut(s)	399, 490, 1166, 1863
NspV	TTCGAA	3 cut(s)	750, 840, 1020
PaeR7I	CTCGAG	2 cut(s)	359, 1771
PciI	ACATGT	2 cut(s)	486, 1162
PciSI	GCTCTTC	1 cut(s)	396
PcsI	WCGNNNNNNNCGW	1 cut(s)	396
PdiI	GCCGGC	1 cut(s)	974
PfeI	GAWTC	1 cut(s)	1810
Pfl23II	CGTACG	1 cut(s)	6
PflMI	CCANNNNNTGG	3 cut(s)	143, 1004, 1369
PkrI	GCNGC	9 cut(s)	331, 542, 583, 629, 704, 794, 884, 1897, 1957
PleI	GAGTC	2 cut(s)	50, 1784
PluTI	GGCGCC	2 cut(s)	1663, 2094
PpsI	GAGTC	2 cut(s)	50, 1784
PpuMI	RGGWCCY	1 cut(s)	159
PscI	ACATGT	2 cut(s)	486, 1162
PshBI	ATTAAT	1 cut(s)	1092
Psp124BI	GAGCTC	2 cut(s)	408, 432
Psp5II	RGGWCCY	1 cut(s)	159
Psp6I	CCWGG	2 cut(s)	1230, 1286
PspEI	GGTNACC	1 cut(s)	1478
PspGI	CCWGG	2 cut(s)	1230, 1286
PspLI	CGTACG	1 cut(s)	6
PspN4I	GGNNCC	8 cut(s)	161, 273, 369, 977, 1543, 1661, 2045, 2092
PspPI	GGNCC	3 cut(s)	159, 1403, 1889
PspPPI	RGGWCCY	1 cut(s)	159
PspXI	VCTCGAGB	1 cut(s)	1771
PstI	CTGCAG	1 cut(s)	1960
PstNI	CAGNNNCTG	1 cut(s)	1892
PsuI	RGATCY	4 cut(s)	271, 367, 844, 1024
RsaI	GTAC	6 cut(s)	8, 279, 317, 1072, 1796, 2071
RsaNI	GTAC	6 cut(s)	7, 278, 316, 1071, 1795, 2070
RseI	CAYNNNNRTG	2 cut(s)	491, 1373
SacI	GAGCTC	2 cut(s)	408, 432
SapI	GCTCTTC	1 cut(s)	396
SaqAI	TTAA	2 cut(s)	480, 1092
SatI	GCNGC	9 cut(s)	330, 541, 582, 628, 703, 793, 883, 1896, 1956
Sau96I	GGNCC	3 cut(s)	159, 1403, 1889
ScaI	AGTACT	1 cut(s)	2071
SchI	GAGTC	2 cut(s)	51, 1784
ScrFI	CCNGG	4 cut(s)	1232, 1288, 1971, 2028
SduI	GDGCHC	5 cut(s)	408, 432, 1469, 1854, 2019
SfaNI	GCATC	7 cut(s)	316, 538, 568, 709, 889, 979, 1555
SfcI	CTRYAG	2 cut(s)	1459, 1956
SfoI	GGCGCC	2 cut(s)	1661, 2092
Sfr274I	CTCGAG	2 cut(s)	359, 1771
SfuI	TTCGAA	3 cut(s)	750, 840, 1020
SinI	GGWCC	2 cut(s)	159, 1403
SlaI	CTCGAG	2 cut(s)	359, 1771
SmiMI	CAYNNNNRTG	2 cut(s)	491, 1373
SmlI	CTYRAG	5 cut(s)	359, 651, 1641, 1771, 1853
SmoI	CTYRAG	5 cut(s)	359, 651, 1641, 1771, 1853
SpeI	ACTAGT	1 cut(s)	566
SspDI	GGCGCC	2 cut(s)	1659, 2090
SspMI	CTAG	4 cut(s)	567, 693, 1239, 2040
SstI	GAGCTC	2 cut(s)	408, 432
StyD4I	CCNGG	4 cut(s)	1230, 1286, 1969, 2026
StyI	CCWWGG	4 cut(s)	111, 642, 1757, 1832
TaaI	ACNGT	3 cut(s)	100, 743, 1345
TaiI	ACGT	2 cut(s)	321, 1809
TatI	WGTACW	2 cut(s)	277, 2069
TauI	GCSGC	3 cut(s)	705, 795, 885
TfiI	GAWTC	1 cut(s)	1810
Tru1I	TTAA	2 cut(s)	480, 1092
Tru9I	TTAA	2 cut(s)	480, 1092
TscAI	CASTG	4 cut(s)	676, 748, 1153, 1348
TseFI	GTSAC	5 cut(s)	94, 737, 917, 1633, 1915
TseI	GCWGC	6 cut(s)	329, 540, 581, 627, 1895, 1955
Tsp45I	GTSAC	5 cut(s)	94, 737, 917, 1633, 1915
TspDTI	ATGAA	5 cut(s)	197, 326, 448, 608, 1357
TspGWI	ACGGA	3 cut(s)	24, 284, 2068
TspRI	CASTG	4 cut(s)	676, 748, 1153, 1348
Van91I	CCANNNNNTGG	3 cut(s)	143, 1004, 1369
VpaK11BI	GGWCC	2 cut(s)	159, 1403
VspI	ATTAAT	1 cut(s)	1092
XapI	RAATTY	4 cut(s)	746, 836, 926, 1016
XceI	RCATGY	4 cut(s)	399, 490, 1166, 1863
XhoI	CTCGAG	2 cut(s)	359, 1771
XspI	CTAG	4 cut(s)	567, 693, 1239, 2040
ZrmI	AGTACT	1 cut(s)	2071
Zsp2I	ATGCAT	1 cut(s)	621

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.