MD13G1164700.v1.1

Acts in the modification of cell walls via demethylesterification of cell wall pectin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000809
Physical Location & Seq
Forward (+)
12993425 .. 12993908
484 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1164700.v1.1.491

Sequence Viewer

Length: 258 bp
ATGCAGTCCCAGATCGATGACGTGATCGCTCCAGAAGGGTGGATGGAATGGATTGGATCAAACAATCACCAATCTTGCTGGCTCGGTGAGTTTGGCAACAGGGGACTCGGTGCCGATCAGAGCAAGAGGGCGACATGGCGTGGAATTAAGAAGCTTACCGCTCAGCACGCCGCGGACTTCACCGCTGGGAGGTTCGTGTTCGGTGATAGATGGATTCAGCCTAGTGGTGTGCCCTATGTCGCTGGCATGATGCCGTAA

Protein Analysis

86

Amino Acids

9.47

Weight (kDa)

6.71

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 1 - 69 7.1e-17 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 161
AccII CGCG 1 cut(s) 173
AciI CCGC 4 cut(s) 159, 171, 173, 183
AclWI GGATC 1 cut(s) 64
AfiI CCNNNNNNNGG 1 cut(s) 189
AjiI CACGTC 1 cut(s) 22
AluBI AGCT 1 cut(s) 154
AluI AGCT 1 cut(s) 154
AlwI GGATC 1 cut(s) 64
AsuHPI GGTGA 4 cut(s) 59, 98, 172, 215
BaeGI GKGCMC 1 cut(s) 234
BanI GGYRCC 1 cut(s) 110
BccI CCATC 2 cut(s) 37, 204
BceAI ACGGC 1 cut(s) 238
BfaI CTAG 1 cut(s) 222
BisI GCNGC 1 cut(s) 171
BlpI GCTNAGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 172
BmgBI CACGTC 1 cut(s) 22
BmiI GGNNCC 1 cut(s) 112
BmsI GCATC 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 15
Bpu1102I GCTNAGC 1 cut(s) 162
Bsa29I ATCGAT 1 cut(s) 15
BsaJI CCNNGG 1 cut(s) 171
Bsc4I CCNNNNNNNGG 1 cut(s) 189
BseCI ATCGAT 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 1 cut(s) 48
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 176
BseSI GKGCMC 1 cut(s) 234
BseYI CCCAGC 1 cut(s) 185
Bsh1236I CGCG 1 cut(s) 173
BshNI GGYRCC 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 15
BslFI GGGAC 1 cut(s) 117
BslI CCNNNNNNNGG 1 cut(s) 189
BsmFI GGGAC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 4 cut(s) 12, 24, 56, 115
Bsp1720I GCTNAGC 1 cut(s) 162
BspACI CCGC 4 cut(s) 159, 171, 173, 183
BspCNI CTCAG 1 cut(s) 175
BspDI ATCGAT 1 cut(s) 15
BspFNI CGCG 1 cut(s) 173
BspLI GGNNCC 1 cut(s) 112
BspPI GGATC 1 cut(s) 64
BspT107I GGYRCC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 161
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 4 cut(s) 12, 24, 56, 115
BstC8I GCNNGC 3 cut(s) 80, 168, 244
BstDEI CTNAG 1 cut(s) 162
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 1 cut(s) 48
BstFNI CGCG 1 cut(s) 173
BstKTI GATC 4 cut(s) 15, 27, 59, 118
BstMBI GATC 4 cut(s) 12, 24, 56, 115
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstSLI GKGCMC 1 cut(s) 234
BstUI CGCG 1 cut(s) 173
BstXI CCANNNNNNTGG 1 cut(s) 39
Bsu15I ATCGAT 1 cut(s) 15
BsuTUI ATCGAT 1 cut(s) 15
BtgI CCRYGG 1 cut(s) 171
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 1 cut(s) 48
Cac8I GCNNGC 3 cut(s) 80, 168, 244
Cfr42I CCGCGG 1 cut(s) 174
ClaI ATCGAT 1 cut(s) 15
CviAII CATG 2 cut(s) 135, 247
CviJI RGCY 3 cut(s) 82, 154, 220
CviKI_1 RGCY 3 cut(s) 82, 154, 220
DdeI CTNAG 1 cut(s) 162
DpnI GATC 4 cut(s) 14, 26, 58, 117
DpnII GATC 4 cut(s) 12, 24, 56, 115
FaeI CATG 2 cut(s) 138, 250
FaiI YATR 3 cut(s) 136, 237, 248
FaqI GGGAC 1 cut(s) 117
FatI CATG 2 cut(s) 134, 246
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 1 cut(s) 55
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 1 cut(s) 222
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 189
GsuI CTGGAG 1 cut(s) 15
Hin1II CATG 2 cut(s) 138, 250
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 2 cut(s) 105, 214
HphI GGTGA 4 cut(s) 59, 98, 172, 215
Hpy188I TCNGA 1 cut(s) 120
Hpy188III TCNNGA 1 cut(s) 32
HpyAV CCTTC 1 cut(s) 29
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 2 cut(s) 138, 250
KspI CCGCGG 1 cut(s) 174
Kzo9I GATC 4 cut(s) 12, 24, 56, 115
LmnI GCTCC 1 cut(s) 34
LpnPI CCDG 6 cut(s) 23, 45, 64, 85, 171, 228
LweI GCATC 1 cut(s) 240
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 21
MalI GATC 4 cut(s) 14, 26, 58, 117
MbiI CCGCTC 1 cut(s) 161
MboI GATC 4 cut(s) 12, 24, 56, 115
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 1 cut(s) 144
MlyI GAGTC 1 cut(s) 99
MnlI CCTC 2 cut(s) 120, 183
MseI TTAA 1 cut(s) 147
MspA1I CMGCKG 2 cut(s) 173, 185
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 1 cut(s) 167
NdeII GATC 4 cut(s) 12, 24, 56, 115
NlaIII CATG 2 cut(s) 138, 250
NlaIV GGNNCC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 214
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 99
PspFI CCCAGC 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 112
SacII CCGCGG 1 cut(s) 174
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 4 cut(s) 12, 24, 56, 115
SchI GAGTC 1 cut(s) 99
SduI GDGCHC 1 cut(s) 234
SetI ASST 3 cut(s) 24, 156, 194
SfaNI GCATC 1 cut(s) 240
Sfr303I CCGCGG 1 cut(s) 174
SgrBI CCGCGG 1 cut(s) 174
Sse9I AATT 1 cut(s) 144
SsiI CCGC 4 cut(s) 159, 171, 173, 183
SspMI CTAG 1 cut(s) 222
TaiI ACGT 1 cut(s) 24
TaqI TCGA 1 cut(s) 15
TasI AATT 1 cut(s) 144
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 214
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.