Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0000809

Physical Location & Seq

Genomic Coordinates Reverse (-)

45906308 .. 45908726

Gene Structure 2419 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG247000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1782 bp

ATGGGCGTCCCTGGTGGTAATAAGAGGATCATAATCATTACGGTCTCTTCGGCGCTTCTGGTGGCCATGGTGGTCGGTGCGGTGGTTGGTGTGATCCAATACAAAAAACAGGAGCAACGCGGAGGAGGAAGCGGAGACGGAGACGGTGACGGAGTCGGAGACTCTAAACCCGGAGATAAAAACGCCGTCCGTACTTCAAAGCCAATTGTAGCCATTTGCCAGCCCACCAACTACAAACAAGAATGTGAGAATAGTCTGCAAAAGGAGGCCGGAGACAAAAGAGACCCGAAAGATCTCGCAAGAGTGGGGATACAGGTGGCCATTAACAAACTGGATGAGGCTATAAGGAACTCCAAAACCTTGCCAGATCAGGCCAAAGATCCCATGACCAAACAAGCTCTCCATATCTGCAACCAGCTATTGAATGCTTCTATGTACGATTTGAAAATTTCTTTCAATAAAATGGGTGCATTAGATCTCGAAAAGGTTGATGAATACGAGAAAGATGTTGAGGTTTGGCTGAGTGGGGCTGTCAGTCACCAAGCAACTTGCATTGATGAATCTCAGAACACAACCGAAGATGTCGGCAAGAAAATGAAGGAAATTTTGAAGTCCGCTCATGAAAGCACTAGCAATGCACTTGCCATGGTCACCGAAATCACTAACGCCTTGAACTCTCTTCATTTTAAATCGAACCGACGCCGGCTCCTGGCCTCATCGCCGCCATCCAAGAATAACAACAATAATAAGAATAATCAACACAAGCAGAATGGCAACAAAAGTAATCAGGGTCTCCCTTCTTGGCTCACTGGTCGTAAGTTGGATCTTCTGACAGCTACGCCTGAAACCCTTAAGCCCAACGTGACGGTGGCTCAAGATGGAAACAGTAAGTATAAAACCATCAACGAAGCTTTGAAACTGGTCCCAAAGAAGAGCAACGACTTGTTTGTGATTTACGTGAAGGAGGGAGTGTACAAAGAACGTGTGACGGTTGCAGGTATGATGGAAAATGTTGTGATGATTGGTGATGGCCCTACTAAGTCCAGGATCTCCGGTGACATTAACTTTGCTAGCGGAACCAGAACTATGTACACTGCAACAGTTGCTGTAGTTGGAAATTTTTTTGTTGCCAAGGACATAGGGTTTGAGAACACAGCCGGAGCGAAAGGGCGCCAAGCGGTGGCACTGCGTGTTCAATCGGACTTCTCCATCTTCTACAACTGCCACATTGACGGGTACCAAAACACCCTATACGTCCAAGCCTACAGGAATTACTTCCGCGACTGCAACATCACTGGCACCATTGACTTCATCTTCGGTGACGCTGTCTCTCTCTTCCAGAACTGCGAGATTGTGGTGAGGAAGCCAATGGACAATCAGACCTGCATCGTCACTGCCCAAGGAAAGACAGACAAGAATGAGGTCACCGGCATCGTTCTCCAAAACTGCACCATCAGAGGCGACCAAGAGTACGAGAACAAGGGATACCTAGGTCGGCCATGGAAGAATTTCGCAAAAACCGTCGTCATGCAGTCACAGATTGATGATGTGATTCAACCTGAAGGGTACATGCCTTGGGACGGCGCCAAGTTCCACATGACGTCATTTTTCGGGGAGTACGACAACAGGGGACCTGGCGCTGACATGTCCAGCAGAGCCGGATGGCCTAGCATCAAAAAGCTTGATGATAAACAAGCTAAAGCCTACAGCGGTAAAAAGTGGTTTGAGGGTGATAGGTGGGTTAAGCCCAGCGGGGTCCCTTTTGTTTCTGGCATCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

593

Amino Acids

65.32

Weight (kDa)

8.89

Isoelectric Point (pI)

30.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	69 - 216	9.5e-25	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	287 - 578	3.7e-121	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05610 AT3G06830 AT4G15980 AT4G15980 AT5G27870 AT5G49180
fragaria_vesca	FvH4_4g18670 FvH4_4g18670 FvH4_4g18670 FvH4_4g18690 FvH4_4g18690
malus_domestica	MD04G1198100.v1.1 MD12G1210700.v1.1 MD13G1164500.v1.1 MD13G1164600.v1.1 MD13G1164700.v1.1 MD13G1164800.v1.1 MD16G1164200.v1.1 MD16G1164300.v1.1
prunus_persica	Prupe.1G123500_v2.0.a1 Prupe.1G123700_v2.0.a1 Prupe.1G123800_v2.0.a1 Prupe.1G131900_v2.0.a1 Prupe.1G132000_v2.0.a1 Prupe.1G132300_v2.0.a1 Prupe.6G318600_v2.0.a1
pyrus_communis	pycom13g14040
rosa_chinensis	RchiOBHm_Chr2g0115181 RchiOBHm_Chr3g0456331 RchiOBHm_Chr4g0423371 RchiOBHm_Chr4g0423391
rosa_laevigata	RLG00000007564 RLG00000007566 RLG00000018164 RLG00000025312
rosa_multiflora	Rmu_co8436939.1_g000001 Rmu_sc0002607.1_g000023 Rmu_sc0002784.1_g000024 Rmu_sc0005307.1_g000019 Rmu_sc0018054.1_g000005 Rmu_sc0031272.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130660 Rroxscaffold_5G00365560 Rroxscaffold_5G00365590
rosa_rugosa	Rorug02G0194200 Rorug04G0186900 Rorug04G0187000 Rorug04G0187100 Rorug04G0187200
rosa_samantha	Rh2AG252700 Rh2BG262300 Rh2CG257300 Rh2DG260900 Rh3BG074700 Rh3CG074300 Rh3DG076200 Rh4AG246000 Rh4AG246100 Rh4BG251500 Rh4BG251600 Rh4CG259800 Rh4CG260100 Rh4DG246400 Rh4DG247000
rosa_wichuraiana	Rw2G019710 Rw3G006230 Rw4G021140 Rw4G021150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1604
Acc36I	ACCTGC	2 cut(s)	986, 1391
Acc65I	GGTACC	1 cut(s)	1236
AccB1I	GGYRCC	4 cut(s)	1170, 1236, 1298, 1583
AccB7I	CCANNNNNTGG	1 cut(s)	1180
AccBSI	CCGCTC	1 cut(s)	617
AccII	CGCG	2 cut(s)	120, 1281
AclWI	GGATC	5 cut(s)	35, 88, 374, 831, 1055
AcoI	YGGCCR	3 cut(s)	63, 318, 1496
AcsI	RAATTY	4 cut(s)	447, 603, 1117, 1507
AcuI	CTGAAG	1 cut(s)	1581
AcyI	GRCGYC	5 cut(s)	6, 700, 1171, 1584, 1601
AdeI	CACNNNGTG	1 cut(s)	1190
AfaI	GTAC	8 cut(s)	193, 437, 974, 1091, 1238, 1472, 1568, 1619
AfiI	CCNNNNNNNGG	3 cut(s)	709, 1180, 1580
AflII	CTTAAG	1 cut(s)	851
AflIII	ACRYGT	2 cut(s)	982, 1644
AgsI	TTSAA	9 cut(s)	198, 424, 445, 457, 610, 673, 916, 1196, 1556
AjnI	CCWGG	4 cut(s)	10, 708, 1043, 1633
AluBI	AGCT	6 cut(s)	398, 418, 836, 911, 1681, 1697
AluI	AGCT	6 cut(s)	398, 418, 836, 911, 1681, 1697
Alw26I	GTCTC	8 cut(s)	49, 129, 135, 153, 267, 276, 797, 1333
AlwI	GGATC	5 cut(s)	35, 88, 374, 831, 1055
AlwNI	CAGNNNCTG	1 cut(s)	1106
AoxI	GGCC	8 cut(s)	63, 267, 318, 372, 711, 1030, 1496, 1664
ApoI	RAATTY	4 cut(s)	447, 603, 1117, 1507
ArsI	GACNNNNNNTTYG	6 cut(s)	1049, 1081, 1127, 1159, 1298, 1330
Asp700I	GAANNNNTTC	2 cut(s)	1274, 1508
Asp718I	GGTACC	1 cut(s)	1236
AspA2I	CCTAGG	1 cut(s)	1489
AspLEI	GCGC	4 cut(s)	55, 1173, 1586, 1640
AspS9I	GGNCC	4 cut(s)	922, 1031, 1631, 1756
AsuC2I	CCSGG	1 cut(s)	171
AsuHPI	GGTGA	9 cut(s)	158, 530, 643, 1037, 1067, 1331, 1369, 1417, 1742
AsuNHI	GCTAGC	1 cut(s)	1070
AvaII	GGWCC	3 cut(s)	922, 1631, 1756
AvrII	CCTAGG	1 cut(s)	1489
BaeI	ACNNNNGTAYC	2 cut(s)	1477, 1510
BalI	TGGCCA	2 cut(s)	65, 320
BanI	GGYRCC	4 cut(s)	1170, 1236, 1298, 1583
BccI	CCATC	8 cut(s)	733, 872, 908, 997, 1022, 1217, 1460, 1656
BceAI	ACGGC	2 cut(s)	170, 1597
BciT130I	CCWGG	4 cut(s)	12, 710, 1045, 1635
BciVI	GTATCC	2 cut(s)	303, 1478
BcnI	CCSGG	1 cut(s)	171
BcoDI	GTCTC	8 cut(s)	49, 129, 135, 153, 267, 276, 797, 1333
BfaI	CTAG	4 cut(s)	630, 1071, 1490, 1668
BfmI	CTRYAG	3 cut(s)	1107, 1264, 1705
BfoI	RGCGCY	4 cut(s)	56, 1174, 1587, 1641
BfrI	CTTAAG	1 cut(s)	851
BfuAI	ACCTGC	2 cut(s)	986, 1391
BfuI	GTATCC	2 cut(s)	303, 1478
BglII	AGATCT	2 cut(s)	292, 475
BisI	GCNGC	1 cut(s)	722
BlnI	CCTAGG	1 cut(s)	1489
BlsI	GCNGC	1 cut(s)	723
Bme1390I	CCNGG	5 cut(s)	12, 171, 710, 1045, 1635
Bme18I	GGWCC	3 cut(s)	922, 1631, 1756
BmgT120I	GGNCC	4 cut(s)	922, 1031, 1631, 1756
BmrFI	CCNGG	5 cut(s)	12, 171, 710, 1045, 1635
BmsI	GCATC	3 cut(s)	1395, 1440, 1680
BmtI	GCTAGC	1 cut(s)	1074
BpuEI	CTTGAG	1 cut(s)	858
BpuMI	CCSGG	1 cut(s)	171
BsaAI	YACGTR	1 cut(s)	958
BsaBI	GATNNNNATC	1 cut(s)	32
BsaHI	GRCGYC	5 cut(s)	6, 700, 1171, 1584, 1601
BsaI	GGTCTC	3 cut(s)	49, 276, 797
BsaJI	CCNNGG	8 cut(s)	10, 66, 645, 1131, 1399, 1489, 1499, 1574
BsaWI	WCCGGW	1 cut(s)	1052
BsaXI	ACNNNNNCTCC	6 cut(s)	384, 414, 690, 720, 956, 986
Bsc4I	CCNNNNNNNGG	3 cut(s)	709, 1180, 1580
Bse118I	RCCGGY	2 cut(s)	702, 1427
Bse1I	ACTGG	4 cut(s)	336, 814, 924, 1300
Bse3DI	GCAATG	1 cut(s)	640
Bse8I	GATNNNNATC	1 cut(s)	32
BseBI	CCWGG	4 cut(s)	12, 710, 1045, 1635
BseDI	CCNNGG	8 cut(s)	10, 66, 645, 1131, 1399, 1489, 1499, 1574
BseGI	GGATG	3 cut(s)	340, 725, 1667
BseJI	GATNNNNATC	1 cut(s)	32
BseLI	CCNNNNNNNGG	3 cut(s)	709, 1180, 1580
BseMI	GCAATG	1 cut(s)	640
BseMII	CTCAG	2 cut(s)	512, 578
BseNI	ACTGG	4 cut(s)	336, 814, 924, 1300
BseRI	GAGGAG	1 cut(s)	138
BseYI	CCCAGC	1 cut(s)	1748
BsgI	GTGCAG	1 cut(s)	1432
Bsh1236I	CGCG	2 cut(s)	120, 1281
BshFI	GGCC	8 cut(s)	65, 269, 320, 374, 713, 1032, 1498, 1666
BshNI	GGYRCC	4 cut(s)	1170, 1236, 1298, 1583
BsiSI	CCGG	7 cut(s)	171, 270, 703, 1053, 1158, 1428, 1659
BslFI	GGGAC	4 cut(s)	908, 1592, 1644, 1742
BslI	CCNNNNNNNGG	3 cut(s)	709, 1180, 1580
BsmAI	GTCTC	8 cut(s)	49, 129, 135, 153, 267, 276, 797, 1333
BsmBI	CGTCTC	2 cut(s)	129, 135
BsmFI	GGGAC	4 cut(s)	908, 1592, 1644, 1742
BsmI	GAATGC	1 cut(s)	430
BsnI	GGCC	8 cut(s)	65, 269, 320, 374, 713, 1032, 1498, 1666
Bso31I	GGTCTC	3 cut(s)	49, 276, 797
Bsp1407I	TGTACA	2 cut(s)	972, 1089
Bsp143I	GATC	8 cut(s)	27, 93, 292, 367, 379, 475, 823, 1047
Bsp19I	CCATGG	3 cut(s)	66, 645, 1499
BspANI	GGCC	8 cut(s)	65, 269, 320, 374, 713, 1032, 1498, 1666
BspCNI	CTCAG	2 cut(s)	513, 577
BspFNI	CGCG	2 cut(s)	120, 1281
BspHI	TCATGA	1 cut(s)	619
BspMI	ACCTGC	2 cut(s)	986, 1391
BspOI	GCTAGC	1 cut(s)	1074
BspPI	GGATC	5 cut(s)	35, 88, 374, 831, 1055
BspQI	GCTCTTC	1 cut(s)	926
BspT107I	GGYRCC	4 cut(s)	1170, 1236, 1298, 1583
BspTI	CTTAAG	1 cut(s)	851
BspTNI	GGTCTC	3 cut(s)	49, 276, 797
BsrBI	CCGCTC	1 cut(s)	617
BsrDI	GCAATG	1 cut(s)	640
BsrFI	RCCGGY	2 cut(s)	702, 1427
BsrGI	TGTACA	2 cut(s)	972, 1089
BsrI	ACTGG	4 cut(s)	336, 814, 924, 1300
BssAI	RCCGGY	2 cut(s)	702, 1427
BssECI	CCNNGG	8 cut(s)	10, 66, 645, 1131, 1399, 1489, 1499, 1574
BssMI	GATC	8 cut(s)	27, 93, 292, 367, 379, 475, 823, 1047
BssNI	GRCGYC	5 cut(s)	6, 700, 1171, 1584, 1601
BssT1I	CCWWGG	7 cut(s)	66, 645, 1131, 1399, 1489, 1499, 1574
Bst2UI	CCWGG	4 cut(s)	12, 710, 1045, 1635
Bst4CI	ACNGT	7 cut(s)	43, 146, 868, 887, 991, 1102, 1522
Bst6I	CTCTTC	4 cut(s)	52, 684, 926, 1340
BstACI	GRCGYC	5 cut(s)	6, 700, 1171, 1584, 1601
BstAFI	CTTAAG	1 cut(s)	851
BstAPI	GCANNNNNTGC	1 cut(s)	1103
BstAUI	TGTACA	2 cut(s)	972, 1089
BstBAI	YACGTR	1 cut(s)	958
BstC8I	GCNNGC	3 cut(s)	221, 704, 1072
BstDEI	CTNAG	3 cut(s)	521, 564, 1038
BstDSI	CCRYGG	3 cut(s)	66, 645, 1499
BstEII	GGTNACC	2 cut(s)	649, 1423
BstF5I	GGATG	3 cut(s)	340, 725, 1667
BstFNI	CGCG	2 cut(s)	120, 1281
BstH2I	RGCGCY	4 cut(s)	56, 1174, 1587, 1641
BstHHI	GCGC	4 cut(s)	55, 1173, 1586, 1640
BstKTI	GATC	8 cut(s)	30, 96, 295, 370, 382, 478, 826, 1050
BstMAI	GTCTC	8 cut(s)	49, 129, 135, 153, 267, 276, 797, 1333
BstMBI	GATC	8 cut(s)	27, 93, 292, 367, 379, 475, 823, 1047
BstMWI	GCNNNNNNNGC	1 cut(s)	1103
BstNI	CCWGG	4 cut(s)	12, 710, 1045, 1635
BstNSI	RCATGY	2 cut(s)	1573, 1648
BstPI	GGTNACC	2 cut(s)	649, 1423
BstSCI	CCNGG	5 cut(s)	10, 169, 708, 1043, 1633
BstSFI	CTRYAG	3 cut(s)	1107, 1264, 1705
BstUI	CGCG	2 cut(s)	120, 1281
BstX2I	RGATCY	5 cut(s)	292, 379, 475, 823, 1047
BstYI	RGATCY	5 cut(s)	292, 379, 475, 823, 1047
BsuI	GTATCC	2 cut(s)	303, 1478
BsuRI	GGCC	8 cut(s)	65, 269, 320, 374, 713, 1032, 1498, 1666
BtgI	CCRYGG	3 cut(s)	66, 645, 1499
BtgZI	GCGATG	1 cut(s)	702
BtsCI	GGATG	3 cut(s)	340, 725, 1667
BtsI	GCAGTG	3 cut(s)	1092, 1184, 1392
BtsIMutI	CAGTG	5 cut(s)	807, 1092, 1184, 1293, 1392
BveI	ACCTGC	2 cut(s)	986, 1391
Cac8I	GCNNGC	3 cut(s)	221, 704, 1072
CaiI	CAGNNNCTG	1 cut(s)	1106
CciI	TCATGA	1 cut(s)	619
CfoI	GCGC	4 cut(s)	55, 1173, 1586, 1640
Cfr10I	RCCGGY	2 cut(s)	702, 1427
Cfr13I	GGNCC	4 cut(s)	922, 1031, 1631, 1756
CseI	GACGC	2 cut(s)	708, 1331
Csp6I	GTAC	8 cut(s)	192, 436, 973, 1090, 1237, 1471, 1567, 1618
CviQI	GTAC	8 cut(s)	192, 436, 973, 1090, 1237, 1471, 1567, 1618
DdeI	CTNAG	3 cut(s)	521, 564, 1038
DinI	GGCGCC	2 cut(s)	1172, 1585
DpnI	GATC	8 cut(s)	29, 95, 294, 369, 381, 477, 825, 1049
DpnII	GATC	8 cut(s)	27, 93, 292, 367, 379, 475, 823, 1047
DraI	TTTAAA	1 cut(s)	688
DraIII	CACNNNGTG	1 cut(s)	1190
EaeI	YGGCCR	3 cut(s)	63, 318, 1496
Eam1104I	CTCTTC	4 cut(s)	52, 684, 926, 1340
EarI	CTCTTC	4 cut(s)	52, 684, 926, 1340
Eco130I	CCWWGG	7 cut(s)	66, 645, 1131, 1399, 1489, 1499, 1574
Eco31I	GGTCTC	3 cut(s)	49, 276, 797
Eco47I	GGWCC	3 cut(s)	922, 1631, 1756
Eco57I	CTGAAG	1 cut(s)	1581
Eco91I	GGTNACC	2 cut(s)	649, 1423
EcoO109I	RGGNCCY	2 cut(s)	1631, 1756
EcoO65I	GGTNACC	2 cut(s)	649, 1423
EcoRII	CCWGG	4 cut(s)	10, 708, 1043, 1633
EcoT14I	CCWWGG	7 cut(s)	66, 645, 1131, 1399, 1489, 1499, 1574
EgeI	GGCGCC	2 cut(s)	1172, 1585
EheI	GGCGCC	2 cut(s)	1172, 1585
ErhI	CCWWGG	7 cut(s)	66, 645, 1131, 1399, 1489, 1499, 1574
Esp3I	CGTCTC	2 cut(s)	129, 135
FaqI	GGGAC	4 cut(s)	908, 1592, 1644, 1742
FauI	CCCGC	1 cut(s)	1745
Fnu4HI	GCNGC	1 cut(s)	722
FokI	GGATG	3 cut(s)	347, 712, 1674
Fsp4HI	GCNGC	1 cut(s)	722
FspBI	CTAG	4 cut(s)	630, 1071, 1490, 1668
GlaI	GCGC	4 cut(s)	54, 1172, 1585, 1639
GluI	GCNGC	1 cut(s)	722
GsaI	CCCAGC	1 cut(s)	1752
HaeII	RGCGCY	4 cut(s)	56, 1174, 1587, 1641
HaeIII	GGCC	8 cut(s)	65, 269, 320, 374, 713, 1032, 1498, 1666
HapII	CCGG	7 cut(s)	171, 270, 703, 1053, 1158, 1428, 1659
HgaI	GACGC	2 cut(s)	708, 1331
HhaI	GCGC	4 cut(s)	55, 1173, 1586, 1640
Hin1I	GRCGYC	5 cut(s)	6, 700, 1171, 1584, 1601
Hin6I	GCGC	4 cut(s)	53, 1171, 1584, 1638
HinP1I	GCGC	4 cut(s)	53, 1171, 1584, 1638
HindIII	AAGCTT	2 cut(s)	909, 1679
HinfI	GANTC	4 cut(s)	153, 161, 560, 1552
HpaII	CCGG	7 cut(s)	171, 270, 703, 1053, 1158, 1428, 1659
HphI	GGTGA	9 cut(s)	158, 530, 643, 1037, 1067, 1331, 1369, 1417, 1742
Hpy166II	GTNNAC	2 cut(s)	973, 1092
Hpy188I	TCNGA	6 cut(s)	158, 567, 831, 1201, 1380, 1457
Hpy188III	TCNNGA	4 cut(s)	479, 620, 875, 1339
Hpy8I	GTNNAC	2 cut(s)	973, 1092
Hpy99I	CGWCG	2 cut(s)	702, 1526
HpyAV	CCTTC	4 cut(s)	592, 807, 955, 1556
HpyCH4III	ACNGT	7 cut(s)	43, 146, 868, 887, 991, 1102, 1522
HpyCH4IV	ACGT	5 cut(s)	861, 957, 982, 1254, 1601
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1103
HpyF3I	CTNAG	3 cut(s)	521, 564, 1038
HpySE526I	ACGT	5 cut(s)	861, 957, 982, 1254, 1601
Hsp92I	GRCGYC	5 cut(s)	6, 700, 1171, 1584, 1601
HspAI	GCGC	4 cut(s)	53, 1171, 1584, 1638
KasI	GGCGCC	2 cut(s)	1170, 1583
KflI	GGGWCCC	1 cut(s)	1756
KpnI	GGTACC	1 cut(s)	1240
KroI	GCCGGC	1 cut(s)	702
KroNI	GCCGGC	1 cut(s)	704
Kzo9I	GATC	8 cut(s)	27, 93, 292, 367, 379, 475, 823, 1047
LguI	GCTCTTC	1 cut(s)	926
LmnI	GCTCC	3 cut(s)	112, 711, 1160
LweI	GCATC	3 cut(s)	1395, 1440, 1680
MaeI	CTAG	4 cut(s)	630, 1071, 1490, 1668
MaeII	ACGT	5 cut(s)	861, 957, 982, 1254, 1601
MalI	GATC	8 cut(s)	29, 95, 294, 369, 381, 477, 825, 1049
MbiI	CCGCTC	1 cut(s)	617
MboI	GATC	8 cut(s)	27, 93, 292, 367, 379, 475, 823, 1047
MboII	GAAGA	9 cut(s)	39, 590, 671, 818, 943, 1204, 1306, 1327, 1516
MfeI	CAATTG	1 cut(s)	204
MflI	RGATCY	5 cut(s)	292, 379, 475, 823, 1047
MlsI	TGGCCA	2 cut(s)	65, 320
MluCI	AATT	6 cut(s)	204, 447, 603, 1117, 1270, 1507
MluNI	TGGCCA	2 cut(s)	65, 320
Mly113I	GGCGCC	2 cut(s)	1171, 1584
MlyI	GAGTC	2 cut(s)	155, 162
MmeI	TCCRAC	3 cut(s)	136, 801, 1093
Mox20I	TGGCCA	2 cut(s)	65, 320
MroNI	GCCGGC	1 cut(s)	702
MroXI	GAANNNNTTC	2 cut(s)	1274, 1508
MscI	TGGCCA	2 cut(s)	65, 320
MseI	TTAA	5 cut(s)	324, 687, 852, 1062, 1743
Msp20I	TGGCCA	2 cut(s)	65, 320
MspA1I	CMGCKG	2 cut(s)	1710, 1752
MspCI	CTTAAG	1 cut(s)	851
MspI	CCGG	7 cut(s)	171, 270, 703, 1053, 1158, 1428, 1659
MspR9I	CCNGG	5 cut(s)	12, 171, 710, 1045, 1635
MunI	CAATTG	1 cut(s)	204
Mva1269I	GAATGC	1 cut(s)	430
MvaI	CCWGG	4 cut(s)	12, 710, 1045, 1635
MvnI	CGCG	2 cut(s)	120, 1281
MwoI	GCNNNNNNNGC	1 cut(s)	1103
NaeI	GCCGGC	1 cut(s)	704
NarI	GGCGCC	2 cut(s)	1171, 1584
NciI	CCSGG	1 cut(s)	171
NcoI	CCATGG	3 cut(s)	66, 645, 1499
NdeII	GATC	8 cut(s)	27, 93, 292, 367, 379, 475, 823, 1047
NgoMIV	GCCGGC	1 cut(s)	702
NheI	GCTAGC	1 cut(s)	1070
NspI	RCATGY	2 cut(s)	1573, 1648
PagI	TCATGA	1 cut(s)	619
PciI	ACATGT	1 cut(s)	1644
PciSI	GCTCTTC	1 cut(s)	926
PcsI	WCGNNNNNNNCGW	2 cut(s)	1518, 1617
PctI	GAATGC	1 cut(s)	430
PdiI	GCCGGC	1 cut(s)	704
PdmI	GAANNNNTTC	2 cut(s)	1274, 1508
PfeI	GAWTC	2 cut(s)	560, 1552
PflFI	GACNNNGTC	2 cut(s)	152, 1325
PflMI	CCANNNNNTGG	1 cut(s)	1180
PfoI	TCCNGGA	1 cut(s)	1043
PkrI	GCNGC	1 cut(s)	723
PleI	GAGTC	2 cut(s)	155, 161
PluTI	GGCGCC	2 cut(s)	1174, 1587
PpsI	GAGTC	2 cut(s)	155, 161
Ppu21I	YACGTR	1 cut(s)	958
PpuMI	RGGWCCY	2 cut(s)	1631, 1756
PscI	ACATGT	1 cut(s)	1644
Psp5II	RGGWCCY	2 cut(s)	1631, 1756
Psp6I	CCWGG	4 cut(s)	10, 708, 1043, 1633
PspEI	GGTNACC	2 cut(s)	649, 1423
PspFI	CCCAGC	1 cut(s)	1748
PspGI	CCWGG	4 cut(s)	10, 708, 1043, 1633
PspPI	GGNCC	4 cut(s)	922, 1031, 1631, 1756
PspPPI	RGGWCCY	2 cut(s)	1631, 1756
PsrI	GAACNNNNNNTAC	2 cut(s)	1469, 1501
PstNI	CAGNNNCTG	1 cut(s)	1106
PsuI	RGATCY	5 cut(s)	292, 379, 475, 823, 1047
PsyI	GACNNNGTC	2 cut(s)	152, 1325
RsaI	GTAC	8 cut(s)	193, 437, 974, 1091, 1238, 1472, 1568, 1619
RsaNI	GTAC	8 cut(s)	192, 436, 973, 1090, 1237, 1471, 1567, 1618
SapI	GCTCTTC	1 cut(s)	926
SaqAI	TTAA	5 cut(s)	324, 687, 852, 1062, 1743
SatI	GCNGC	1 cut(s)	722
Sau3AI	GATC	8 cut(s)	27, 93, 292, 367, 379, 475, 823, 1047
Sau96I	GGNCC	4 cut(s)	922, 1031, 1631, 1756
SchI	GAGTC	2 cut(s)	155, 162
ScrFI	CCNGG	5 cut(s)	12, 171, 710, 1045, 1635
SfaNI	GCATC	3 cut(s)	1395, 1440, 1680
SfcI	CTRYAG	3 cut(s)	1107, 1264, 1705
SfoI	GGCGCC	2 cut(s)	1172, 1585
SinI	GGWCC	3 cut(s)	922, 1631, 1756
SmlI	CTYRAG	2 cut(s)	851, 873
SmoI	CTYRAG	2 cut(s)	851, 873
Sse9I	AATT	6 cut(s)	204, 447, 603, 1117, 1270, 1507
SspDI	GGCGCC	2 cut(s)	1170, 1583
SspMI	CTAG	4 cut(s)	630, 1071, 1490, 1668
StyD4I	CCNGG	5 cut(s)	10, 169, 708, 1043, 1633
StyI	CCWWGG	7 cut(s)	66, 645, 1131, 1399, 1489, 1499, 1574
TaaI	ACNGT	7 cut(s)	43, 146, 868, 887, 991, 1102, 1522
TaiI	ACGT	5 cut(s)	864, 960, 985, 1257, 1604
TaqI	TCGA	2 cut(s)	480, 692
TasI	AATT	6 cut(s)	204, 447, 603, 1117, 1270, 1507
TatI	WGTACW	2 cut(s)	972, 1089
TauI	GCSGC	1 cut(s)	724
TfiI	GAWTC	2 cut(s)	560, 1552
Tru1I	TTAA	5 cut(s)	324, 687, 852, 1062, 1743
Tru9I	TTAA	5 cut(s)	324, 687, 852, 1062, 1743
TscAI	CASTG	5 cut(s)	814, 1099, 1191, 1300, 1399
TspDTI	ATGAA	6 cut(s)	507, 573, 611, 636, 671, 1300
TspGWI	ACGGA	3 cut(s)	153, 165, 179
TspRI	CASTG	5 cut(s)	814, 1099, 1191, 1300, 1399
Tth111I	GACNNNGTC	2 cut(s)	152, 1325
Van91I	CCANNNNNTGG	1 cut(s)	1180
Vha464I	CTTAAG	1 cut(s)	851
VpaK11BI	GGWCC	3 cut(s)	922, 1631, 1756
XapI	RAATTY	4 cut(s)	447, 603, 1117, 1507
XceI	RCATGY	2 cut(s)	1573, 1648
XcmI	CCANNNNNNNNNTGG	2 cut(s)	328, 865
XmaJI	CCTAGG	1 cut(s)	1489
XmnI	GAANNNNTTC	2 cut(s)	1274, 1508
XspI	CTAG	4 cut(s)	630, 1071, 1490, 1668
ZraI	GACGTC	1 cut(s)	1602

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.