FvH4_4g32450

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002177
Physical Location & Seq
Reverse (-)
31082647 .. 31087027
4381 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32450.t3

Sequence Viewer

Length: 1989 bp
ATGATATCCTGGAATGACCTCTACAAGGTCTTGACGGCGGTGGTGCCTCTCTACGTGGCCATGATCTTAGCCTACGGCTCCGTCCGGTGGTGGAAGATCTTCTCCACTGACCAATGCTCCGGCATCAACCGCTTCGTCGCAATCTTCGCCGTTCCTCTTCTCTCCTTCCACTTCATCTCCACCAACGACCCTTACACCATGAACTTCCGCTTCATAGCCGCCGATACGCTCCAGAAGATCATCATGCTCTTTGCGCTGGCTCTTTGGACCAACTTCACCCGCAACGGAAGCTTAGAATGGATGATCACAATTTTCTCGCTCTCGACGCTTCCCAATACTTTAGTCATGGGAATCCCACTGCTTATCGCCATGTACGGCCACCAAATCCCGGAGGCCGGCAGCCTGATGGTCCAGGTCGTCGTGCTCCAGTGCATCATTTGGTACACACTCCTGCTCTTCCTCTTCGAGTACCGCGGCGCAAAGTTGCTTATCATGGAGCAGTTTCCAGAGACCGCCGCCTCCATCGTCTCCTTCAAAGTCGACTCCGACGTCATGTCCCTTGACGGCCGGGACTTTCTCGAGACCGACGCCGAGATCGGCGACGACGGCAAGCTCCACGTGAAGGTGAGGAAGTCCAACGCGTCGAGACGGTCTCTCTCGGCAATGACGCCTCGACCGTCGAACCTCACCGGAGCCGAGATTTACAGTCTCAGCTCTTCCCGGAACCCAACTCCCCGTGGCTCGAATTTTAATCCTTCCGATTTTTACTCCATGATGGGAGTTCAGGGGTTCAATACCCGCCACTCCAACTTTGGCGCCGGCGACATGTACTCGGTTCAGTCCTCTAGAGGCCCGACTCCGAGACCTTCCAATTTCGAAGAGAATTGTGCTCCAATAATGGCGCAAACTGTTACTTCTCCCCGGTTCGGGTTTTACCCGGCGCAGACTGTCCCGTCGTCGTACCCGGCTCCGAATCCGGAATTCTCCGCTCCGGTCACTAAAACCGCCGCTAAAACTAACCAACAGCCACTCCAACCGCAACCGCCGCCGCAGCAGGTTAACTCCAACTCAAGCAAAGCAAACCACGACGCGAAAGAGCTCCACATGTTTGTTTGGAGCTCCAGCAATTCCCCTGTTTCCGAAGCCGGAGGGCTCCACGTTTTCGGGGGACCTGACTTCGCCGCGTCGGAGCAATCCGGGCGGTCAGATCAGGGCGCCAAGGAGATCAGGATGTTGGTTGCTGATCACCCCCAGAATGGAGAAACCAAAGTGGCAGTTACGGACAATGACGGCGAACACCACCACCATCAGGCGGTAGCAGAAGCCTTTAAATTCGCCGGAAAAGCTGATCAGGGAGAGGAAGATAGAGAAAAGGAGGGGCCAATGGGATTTAATAAGCTGGGGTCAAGCTCCACTGCCGAGCTACACCCAAAAGGTGTAGGAGTTGGTGCTGATCATCACGAGTCTAGCGGAGGGAAACAAATGCCGCCTGCGAGCGTGATGACCCGTCTGATCTTGATCATGGTTTGGCGGAAGCTTATCCGCAACCCCAACACATACTCCAGCCTCATCGGTCTCATTTGGTCACTCGTCTCTTTCCGGTGGCACGTGGCTATGCCGAGCATTATAAAGGGGTCGATCTCCATACTGTCCGATGCCGGCCTTGGAATGGCTATGTTCAGCTTAGGTCTCTTTATGGCACTTCAACCTAAGATAATAGCATGTGGAAACTCGGTGGCCGGGTTTGCAATGGCCGTGAGATTCCTCACCGGCCCGGCTGTGATGGCTGCAGCCTCCATTGCTGTTGGGCTACGAGGCACCCTCCTCCACTTAGCAATTGTCCAGGCTGCACTTCCTCAAGGAATCGTTCCCTTTGTCTTTGCCAAAGAGTACAATGTTCATCCAGCCATTCTCAGCACTGGGGTAATATTCGGAATGCTGATAGCATTACCGATTACGCTAGTGTACTATATCGTTCTCGGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000578 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009914 GO:0009921 GO:0009926 GO:0009942 GO:0009958 GO:0009966 GO:0009986 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0012506 GO:0015562 GO:0016020 GO:0016043 GO:0016049 GO:0016328 GO:0021700 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0030054 GO:0030154 GO:0031090 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048583 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0080161 GO:0090558 GO:0090627 GO:0098796 GO:0098797 GO:0099402 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

663

Amino Acids

72.11

Weight (kDa)

7.77

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 10 - 570 1.3e-90 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1628
AasI GACNNNNNNGTC 2 cut(s) 548, 952
AatII GACGTC 1 cut(s) 552
Acc36I ACCTGC 1 cut(s) 1045
AccB1I GGYRCC 4 cut(s) 43, 815, 1214, 1817
AccBSI CCGCTC 1 cut(s) 989
AccI GTMKAC 1 cut(s) 540
AccII CGCG 4 cut(s) 474, 641, 1091, 1184
AccIII TCCGGA 1 cut(s) 976
AcoI YGGCCR 5 cut(s) 57, 376, 565, 1737, 1752
AcsI RAATTY 3 cut(s) 745, 980, 1331
AcvI CACGTG 2 cut(s) 619, 1609
AcyI GRCGYC 5 cut(s) 549, 588, 668, 816, 1215
AfaI GTAC 7 cut(s) 374, 443, 470, 830, 962, 1892, 1967
AflIII ACRYGT 3 cut(s) 639, 825, 1104
AgsI TTSAA 3 cut(s) 535, 793, 1706
AhdI GACNNNNNGTC 1 cut(s) 553
AjnI CCWGG 3 cut(s) 8, 411, 1842
Alw21I GWGCWC 4 cut(s) 426, 892, 1101, 1121
Ama87I CYCGRG 1 cut(s) 578
Aor13HI TCCGGA 1 cut(s) 976
ApeKI GCWGC 5 cut(s) 399, 1051, 1787, 1790, 1847
ApoI RAATTY 3 cut(s) 745, 980, 1331
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 6 cut(s) 256, 479, 818, 904, 943, 1217
AspS9I GGNCC 6 cut(s) 267, 409, 851, 1169, 1379, 1772
AsuC2I CCSGG 9 cut(s) 389, 569, 721, 922, 938, 965, 1198, 1741, 1775
AsuHPI GGTGA 5 cut(s) 268, 637, 679, 1238, 1759
AsuII TTCGAA 1 cut(s) 876
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 3 cut(s) 267, 409, 1169
BalI TGGCCA 1 cut(s) 59
BanI GGYRCC 4 cut(s) 43, 815, 1214, 1817
BanII GRGCYC 3 cut(s) 1101, 1121, 1155
BauI CACGAG 1 cut(s) 1460
BbrPI CACGTG 2 cut(s) 619, 1609
Bbv12I GWGCWC 4 cut(s) 426, 892, 1101, 1121
BbvI GCAGC 5 cut(s) 411, 1063, 1774, 1802, 1834
BccI CCATC 5 cut(s) 400, 530, 769, 1314, 1777
BceAI ACGGC 8 cut(s) 51, 91, 134, 391, 580, 622, 1306, 1739
BciT130I CCWGG 3 cut(s) 10, 413, 1844
BclI TGATCA 5 cut(s) 303, 1243, 1348, 1453, 1518
BcnI CCSGG 9 cut(s) 389, 569, 721, 922, 938, 965, 1198, 1741, 1775
BfaI CTAG 3 cut(s) 846, 1467, 1961
BfmI CTRYAG 1 cut(s) 1788
BfoI RGCGCY 2 cut(s) 819, 1218
BfuAI ACCTGC 1 cut(s) 1045
BglII AGATCT 1 cut(s) 96
Bme18I GGWCC 3 cut(s) 267, 409, 1169
BmeRI GACNNNNNGTC 1 cut(s) 553
BmeT110I CYCGRG 1 cut(s) 578
BmgT120I GGNCC 6 cut(s) 267, 409, 851, 1169, 1379, 1772
BmrI ACTGGG 1 cut(s) 1929
BmsI GCATC 3 cut(s) 132, 441, 1645
BmuI ACTGGG 1 cut(s) 1929
BplI GAGNNNNNCTC 4 cut(s) 637, 669, 1806, 1838
BpmI CTGGAG 4 cut(s) 215, 410, 1105, 1546
Bpu10I CCTNAGC 1 cut(s) 1684
Bpu14I TTCGAA 1 cut(s) 876
BpuEI CTTGAG 2 cut(s) 1054, 1842
BpuMI CCSGG 9 cut(s) 389, 569, 721, 922, 938, 965, 1198, 1741, 1775
BsaAI YACGTR 3 cut(s) 55, 619, 1609
BsaBI GATNNNNATC 1 cut(s) 1517
BsaHI GRCGYC 5 cut(s) 549, 588, 668, 816, 1215
BsaI GGTCTC 6 cut(s) 503, 575, 657, 856, 1580, 1694
BsaJI CCNNGG 5 cut(s) 472, 736, 920, 1218, 1663
BsaWI WCCGGW 5 cut(s) 84, 689, 976, 991, 1599
BsaXI ACNNNNNCTCC 8 cut(s) 101, 131, 161, 191, 1014, 1044, 1544, 1574
Bse118I RCCGGY 4 cut(s) 395, 818, 1658, 1769
Bse1I ACTGG 2 cut(s) 427, 1924
Bse3DI GCAATG 3 cut(s) 669, 1755, 1797
Bse8I GATNNNNATC 1 cut(s) 1517
BseAI TCCGGA 1 cut(s) 976
BseBI CCWGG 3 cut(s) 10, 413, 1844
BseDI CCNNGG 5 cut(s) 472, 736, 920, 1218, 1663
BseGI GGATG 3 cut(s) 306, 1236, 1900
BseJI GATNNNNATC 1 cut(s) 1517
BseMI GCAATG 3 cut(s) 669, 1755, 1797
BseMII CTCAG 2 cut(s) 724, 1927
BseNI ACTGG 2 cut(s) 427, 1924
BseRI GAGGAG 1 cut(s) 1814
BseX3I CGGCCG 1 cut(s) 565
BseXI GCAGC 5 cut(s) 411, 1063, 1774, 1802, 1834
BseYI CCCAGC 1 cut(s) 1399
BsgI GTGCAG 1 cut(s) 1833
Bsh1236I CGCG 4 cut(s) 474, 641, 1091, 1184
Bsh1285I CGRYCG 2 cut(s) 568, 677
BshNI GGYRCC 4 cut(s) 43, 815, 1214, 1817
BsiEI CGRYCG 2 cut(s) 568, 677
BsiHKAI GWGCWC 4 cut(s) 426, 892, 1101, 1121
BsiHKCI CYCGRG 1 cut(s) 578
BslFI GGGAC 4 cut(s) 541, 584, 935, 1182
BsmBI CGTCTC 3 cut(s) 532, 640, 1597
BsmFI GGGAC 4 cut(s) 541, 584, 935, 1182
BsmI GAATGC 1 cut(s) 1941
Bso31I GGTCTC 6 cut(s) 503, 575, 657, 856, 1580, 1694
BsoBI CYCGRG 1 cut(s) 578
Bsp119I TTCGAA 1 cut(s) 876
Bsp1286I GDGCHC 5 cut(s) 426, 892, 1101, 1121, 1155
Bsp13I TCCGGA 1 cut(s) 976
BspCNI CTCAG 2 cut(s) 723, 1926
BspEI TCCGGA 1 cut(s) 976
BspFNI CGCG 4 cut(s) 474, 641, 1091, 1184
BspMAI CTGCAG 1 cut(s) 1792
BspMI ACCTGC 1 cut(s) 1045
BspQI GCTCTTC 2 cut(s) 461, 721
BspT104I TTCGAA 1 cut(s) 876
BspT107I GGYRCC 4 cut(s) 43, 815, 1214, 1817
BspTNI GGTCTC 6 cut(s) 503, 575, 657, 856, 1580, 1694
BsrBI CCGCTC 1 cut(s) 989
BsrDI GCAATG 3 cut(s) 669, 1755, 1797
BsrFI RCCGGY 4 cut(s) 395, 818, 1658, 1769
BsrI ACTGG 2 cut(s) 427, 1924
BssAI RCCGGY 4 cut(s) 395, 818, 1658, 1769
BssECI CCNNGG 5 cut(s) 472, 736, 920, 1218, 1663
BssNI GRCGYC 5 cut(s) 549, 588, 668, 816, 1215
BssSI CACGAG 1 cut(s) 1460
BssT1I CCWWGG 2 cut(s) 1218, 1663
Bst2BI CACGAG 1 cut(s) 1460
Bst2UI CCWGG 3 cut(s) 10, 413, 1844
Bst4CI ACNGT 6 cut(s) 651, 678, 707, 910, 949, 1650
Bst6I CTCTTC 5 cut(s) 162, 461, 467, 721, 873
BstACI GRCGYC 5 cut(s) 549, 588, 668, 816, 1215
BstBAI YACGTR 3 cut(s) 55, 619, 1609
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 7 cut(s) 258, 397, 611, 820, 1491, 1495, 1660
BstDEI CTNAG 7 cut(s) 67, 292, 710, 1684, 1710, 1831, 1913
BstDSI CCRYGG 2 cut(s) 472, 736
BstENI CCTNNNNNAGG 1 cut(s) 23
BstF5I GGATG 3 cut(s) 306, 1236, 1900
BstFNI CGCG 4 cut(s) 474, 641, 1091, 1184
BstH2I RGCGCY 2 cut(s) 819, 1218
BstHHI GCGC 6 cut(s) 256, 479, 818, 904, 943, 1217
BstMCI CGRYCG 2 cut(s) 568, 677
BstNI CCWGG 3 cut(s) 10, 413, 1844
BstNSI RCATGY 3 cut(s) 829, 1108, 1725
BstSFI CTRYAG 1 cut(s) 1788
BstUI CGCG 4 cut(s) 474, 641, 1091, 1184
BstV1I GCAGC 5 cut(s) 411, 1063, 1774, 1802, 1834
BstX2I RGATCY 1 cut(s) 96
BstYI RGATCY 1 cut(s) 96
BstZI CGGCCG 1 cut(s) 565
BtgI CCRYGG 2 cut(s) 472, 736
BtsCI GGATG 3 cut(s) 306, 1236, 1900
BtsI GCAGTG 2 cut(s) 356, 1413
BtsIMutI CAGTG 5 cut(s) 105, 356, 434, 1413, 1917
BveI ACCTGC 1 cut(s) 1045
Cac8I GCNNGC 7 cut(s) 258, 397, 611, 820, 1491, 1495, 1660
CfoI GCGC 6 cut(s) 256, 479, 818, 904, 943, 1217
Cfr10I RCCGGY 4 cut(s) 395, 818, 1658, 1769
Cfr13I GGNCC 6 cut(s) 267, 409, 851, 1169, 1379, 1772
Cfr42I CCGCGG 1 cut(s) 475
CseI GACGC 6 cut(s) 334, 596, 630, 676, 1097, 1173
Csp6I GTAC 7 cut(s) 373, 442, 469, 829, 961, 1891, 1966
CviQI GTAC 7 cut(s) 373, 442, 469, 829, 961, 1891, 1966
DdeI CTNAG 7 cut(s) 67, 292, 710, 1684, 1710, 1831, 1913
DinI GGCGCC 2 cut(s) 817, 1216
DraI TTTAAA 1 cut(s) 1330
DrdI GACNNNNNNGTC 2 cut(s) 548, 952
DriI GACNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 2 cut(s) 548, 952
EaeI YGGCCR 5 cut(s) 57, 376, 565, 1737, 1752
EagI CGGCCG 1 cut(s) 565
Eam1104I CTCTTC 5 cut(s) 162, 461, 467, 721, 873
Eam1105I GACNNNNNGTC 1 cut(s) 553
EarI CTCTTC 5 cut(s) 162, 461, 467, 721, 873
EciI GGCGGA 1 cut(s) 1546
Ecl136II GAGCTC 2 cut(s) 1099, 1119
EclXI CGGCCG 1 cut(s) 565
Eco130I CCWWGG 2 cut(s) 1218, 1663
Eco24I GRGCYC 3 cut(s) 1101, 1121, 1155
Eco31I GGTCTC 6 cut(s) 503, 575, 657, 856, 1580, 1694
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 3 cut(s) 267, 409, 1169
Eco52I CGGCCG 1 cut(s) 565
Eco53kI GAGCTC 2 cut(s) 1099, 1119
Eco72I CACGTG 2 cut(s) 619, 1609
Eco88I CYCGRG 1 cut(s) 578
EcoICRI GAGCTC 2 cut(s) 1099, 1119
EcoNI CCTNNNNNAGG 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 1169
EcoRI GAATTC 1 cut(s) 980
EcoRII CCWGG 3 cut(s) 8, 411, 1842
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 2 cut(s) 1218, 1663
EcoT38I GRGCYC 3 cut(s) 1101, 1121, 1155
EgeI GGCGCC 2 cut(s) 817, 1216
EheI GGCGCC 2 cut(s) 817, 1216
ErhI CCWWGG 2 cut(s) 1218, 1663
Esp3I CGTCTC 3 cut(s) 532, 640, 1597
FaqI GGGAC 4 cut(s) 541, 584, 935, 1182
FauI CCCGC 2 cut(s) 287, 806
FbaI TGATCA 5 cut(s) 303, 1243, 1348, 1453, 1518
FblI GTMKAC 1 cut(s) 540
FokI GGATG 3 cut(s) 313, 1243, 1887
FriOI GRGCYC 3 cut(s) 1101, 1121, 1155
FspBI CTAG 3 cut(s) 846, 1467, 1961
GlaI GCGC 6 cut(s) 255, 478, 817, 903, 942, 1216
GsaI CCCAGC 1 cut(s) 1403
GsuI CTGGAG 4 cut(s) 215, 410, 1105, 1546
HaeII RGCGCY 2 cut(s) 819, 1218
HgaI GACGC 6 cut(s) 334, 596, 630, 676, 1097, 1173
HhaI GCGC 6 cut(s) 256, 479, 818, 904, 943, 1217
Hin1I GRCGYC 5 cut(s) 549, 588, 668, 816, 1215
Hin6I GCGC 6 cut(s) 254, 477, 816, 902, 941, 1215
HinP1I GCGC 6 cut(s) 254, 477, 816, 902, 941, 1215
HincII GTYRAC 2 cut(s) 541, 1060
HindII GTYRAC 2 cut(s) 541, 1060
HindIII AAGCTT 2 cut(s) 289, 1535
HinfI GANTC 7 cut(s) 351, 542, 856, 973, 1463, 1761, 1863
HpaI GTTAAC 1 cut(s) 1060
HphI GGTGA 5 cut(s) 268, 637, 679, 1238, 1759
Hpy166II GTNNAC 4 cut(s) 444, 541, 1060, 1966
Hpy8I GTNNAC 4 cut(s) 444, 541, 1060, 1966
HpyAV CCTTC 5 cut(s) 175, 541, 616, 765, 876
HpyCH4III ACNGT 6 cut(s) 651, 678, 707, 910, 949, 1650
HpyCH4IV ACGT 5 cut(s) 54, 549, 618, 1158, 1608
HpyCH4V TGCA 4 cut(s) 432, 1748, 1790, 1850
HpyF3I CTNAG 7 cut(s) 67, 292, 710, 1684, 1710, 1831, 1913
HpySE526I ACGT 5 cut(s) 54, 549, 618, 1158, 1608
Hsp92I GRCGYC 5 cut(s) 549, 588, 668, 816, 1215
HspAI GCGC 6 cut(s) 254, 477, 816, 902, 941, 1215
KasI GGCGCC 2 cut(s) 815, 1214
Kpn2I TCCGGA 1 cut(s) 976
KroI GCCGGC 3 cut(s) 395, 818, 1658
KroNI GCCGGC 3 cut(s) 397, 820, 1660
Ksp22I TGATCA 5 cut(s) 303, 1243, 1348, 1453, 1518
KspAI GTTAAC 1 cut(s) 1060
KspI CCGCGG 1 cut(s) 475
LguI GCTCTTC 2 cut(s) 461, 721
Lsp1109I GCAGC 5 cut(s) 411, 1063, 1774, 1802, 1834
LweI GCATC 3 cut(s) 132, 441, 1645
MaeI CTAG 3 cut(s) 846, 1467, 1961
MaeII ACGT 5 cut(s) 54, 549, 618, 1158, 1608
MaeIII GTNAC 4 cut(s) 910, 994, 1276, 1584
MbiI CCGCTC 1 cut(s) 989
MfeI CAATTG 1 cut(s) 1836
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 5 cut(s) 426, 892, 1101, 1121, 1155
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 8 cut(s) 309, 745, 871, 883, 980, 1126, 1331, 1836
MluI ACGCGT 1 cut(s) 639
MluNI TGGCCA 1 cut(s) 59
Mly113I GGCGCC 2 cut(s) 816, 1215
MlyI GAGTC 3 cut(s) 536, 850, 1472
MmeI TCCRAC 6 cut(s) 570, 660, 831, 1057, 1089, 1167
Mox20I TGGCCA 1 cut(s) 59
MreI CGCCGGCG 1 cut(s) 818
MroI TCCGGA 1 cut(s) 976
MroNI GCCGGC 3 cut(s) 395, 818, 1658
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 1 cut(s) 59
MseI TTAA 4 cut(s) 750, 1059, 1329, 1392
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 474
MunI CAATTG 1 cut(s) 1836
Mva1269I GAATGC 1 cut(s) 1941
MvaI CCWGG 3 cut(s) 10, 413, 1844
MvnI CGCG 4 cut(s) 474, 641, 1091, 1184
NaeI GCCGGC 3 cut(s) 397, 820, 1660
NarI GGCGCC 2 cut(s) 816, 1215
NciI CCSGG 9 cut(s) 389, 569, 721, 922, 938, 965, 1198, 1741, 1775
NgoMIV GCCGGC 3 cut(s) 395, 818, 1658
NmeAIII GCCGAG 5 cut(s) 616, 638, 721, 1444, 1644
NmuCI GTSAC 2 cut(s) 994, 1584
NspI RCATGY 3 cut(s) 829, 1108, 1725
NspV TTCGAA 1 cut(s) 876
PaeR7I CTCGAG 1 cut(s) 578
PciI ACATGT 2 cut(s) 825, 1104
PciSI GCTCTTC 2 cut(s) 461, 721
PcsI WCGNNNNNNNCGW 2 cut(s) 546, 674
PctI GAATGC 1 cut(s) 1941
PdiI GCCGGC 3 cut(s) 397, 820, 1660
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 4 cut(s) 351, 973, 1761, 1863
PfoI TCCNGGA 3 cut(s) 8, 387, 719
PleI GAGTC 3 cut(s) 536, 850, 1471
PluTI GGCGCC 2 cut(s) 819, 1218
PmaCI CACGTG 2 cut(s) 619, 1609
PmlI CACGTG 2 cut(s) 619, 1609
PpsI GAGTC 3 cut(s) 536, 850, 1471
Ppu21I YACGTR 3 cut(s) 55, 619, 1609
PpuMI RGGWCCY 1 cut(s) 1169
PscI ACATGT 2 cut(s) 825, 1104
PsiI TTATAA 1 cut(s) 1628
Psp124BI GAGCTC 2 cut(s) 1101, 1121
Psp5II RGGWCCY 1 cut(s) 1169
Psp6I CCWGG 3 cut(s) 8, 411, 1842
PspCI CACGTG 2 cut(s) 619, 1609
PspFI CCCAGC 1 cut(s) 1399
PspGI CCWGG 3 cut(s) 8, 411, 1842
PspPI GGNCC 6 cut(s) 267, 409, 851, 1169, 1379, 1772
PspPPI RGGWCCY 1 cut(s) 1169
PstI CTGCAG 1 cut(s) 1792
PsuI RGATCY 1 cut(s) 96
RsaI GTAC 7 cut(s) 374, 443, 470, 830, 962, 1892, 1967
RsaNI GTAC 7 cut(s) 373, 442, 469, 829, 961, 1891, 1966
SacI GAGCTC 2 cut(s) 1101, 1121
SacII CCGCGG 1 cut(s) 475
SalI GTCGAC 1 cut(s) 539
SapI GCTCTTC 2 cut(s) 461, 721
SaqAI TTAA 4 cut(s) 750, 1059, 1329, 1392
Sau96I GGNCC 6 cut(s) 267, 409, 851, 1169, 1379, 1772
SchI GAGTC 3 cut(s) 536, 850, 1472
SduI GDGCHC 5 cut(s) 426, 892, 1101, 1121, 1155
SfaNI GCATC 3 cut(s) 132, 441, 1645
SfcI CTRYAG 1 cut(s) 1788
SfoI GGCGCC 2 cut(s) 817, 1216
Sfr274I CTCGAG 1 cut(s) 578
Sfr303I CCGCGG 1 cut(s) 475
SfuI TTCGAA 1 cut(s) 876
SgrAI CRCCGGYG 1 cut(s) 818
SgrBI CCGCGG 1 cut(s) 475
SinI GGWCC 3 cut(s) 267, 409, 1169
SlaI CTCGAG 1 cut(s) 578
SmlI CTYRAG 3 cut(s) 578, 1069, 1857
SmoI CTYRAG 3 cut(s) 578, 1069, 1857
Sse9I AATT 8 cut(s) 309, 745, 871, 883, 980, 1126, 1331, 1836
SspDI GGCGCC 2 cut(s) 815, 1214
SspI AATATT 1 cut(s) 1929
SspMI CTAG 3 cut(s) 846, 1467, 1961
SstI GAGCTC 2 cut(s) 1101, 1121
StyI CCWWGG 2 cut(s) 1218, 1663
TaaI ACNGT 6 cut(s) 651, 678, 707, 910, 949, 1650
TaiI ACGT 5 cut(s) 57, 552, 621, 1161, 1611
TaqII GACCGA 2 cut(s) 599, 1562
TasI AATT 8 cut(s) 309, 745, 871, 883, 980, 1126, 1331, 1836
TatI WGTACW 3 cut(s) 828, 1890, 1965
TauI GCSGC 8 cut(s) 221, 477, 518, 1010, 1048, 1051, 1184, 1489
TfiI GAWTC 4 cut(s) 351, 973, 1761, 1863
Tru1I TTAA 4 cut(s) 750, 1059, 1329, 1392
Tru9I TTAA 4 cut(s) 750, 1059, 1329, 1392
TscAI CASTG 5 cut(s) 112, 363, 434, 1420, 1924
TseFI GTSAC 2 cut(s) 994, 1584
TseI GCWGC 5 cut(s) 399, 1051, 1787, 1790, 1847
Tsp45I GTSAC 2 cut(s) 994, 1584
TspDTI ATGAA 4 cut(s) 163, 202, 215, 1889
TspGWI ACGGA 3 cut(s) 70, 300, 1295
TspRI CASTG 5 cut(s) 112, 363, 434, 1420, 1924
VpaK11BI GGWCC 3 cut(s) 267, 409, 1169
XagI CCTNNNNNAGG 1 cut(s) 23
XapI RAATTY 3 cut(s) 745, 980, 1331
XbaI TCTAGA 1 cut(s) 845
XceI RCATGY 3 cut(s) 829, 1108, 1725
XcmI CCANNNNNNNNNTGG 1 cut(s) 809
XhoI CTCGAG 1 cut(s) 578
XmiI GTMKAC 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 3 cut(s) 846, 1467, 1961
ZraI GACGTC 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.