MD13G1025300.v1.1

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002177
Physical Location & Seq
Forward (+)
1786086 .. 1792173
6088 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1025300.v1.1.491

Sequence Viewer

Length: 1983 bp
ATGATTTCGTGGCACGACCTTTACAAAGTCTTGACGGCGGTGGTGCCGCTGTACGTGGCCATGATCTTAGCCTACGGCTCCGTCCGGTGGTGGAAGATCTTCTCCCCCGACCAGTGCTCCGGCATCAACCGCTTCGTCGCGATATTTGCCGTCCCCTTGCTCTCCTTCCACTTCATCTCCACCAACGACCCCTACACCATGAACTTCCGGTTCATCGCCGCCGATACCCTCCAGAAAATCATCATGCTCTTTGCCCTCGGCCTCTGGACCAACTTCACCCGCAACGGCAGCCTCGAGTGGATGATTACAATTTTCTCCCTCTCTACTCTCCCCAACACTCTCGTCATGGGGATTCCGCTGTTAATCGCCATGTACGGCCATAAAATCCCGGAATCCGGCAGCCTCATGGTGCAGGTGGTGGTACTGCAGTGCATCATCTGGTACACTCTCCTCCTCTTCCTCTTCGAGTACCGTGGCGCCAAGCTTCTAATCATGGAGCAGTTCCCGGAGACCGCCGCCTCCATCGTCTCCTTCAAAGTCGACTCCGACGTCGTATCGCTCGACGGTCGCGATTTTCTCGAGACCGACGCCGAAATCGGCGACGACGGAAAGCTCCACGTCAAGGTCCGGAAGTCCAACGCATCAAGGCGGTCACTCTCCGCAATGACCCCTCGGCCGTCGAACCTCACCGGCGCCGAGATTTACAGCCTGAGCTCCTCCCGAAACCCGACGCCGAGAGGCTCCAATTTCAACCCCTCCGATTTTTACTCCATGATGGGAGTCCAAGGGTTCAACACCCGGCACTCCAATTTCGGACCCGCCGACTTGTATTCGGTTCAGTCCTCCCGCGGTCCGACTCCCCGACCCTCCAATTTCGAAGAGAATTGCGCCGCTCAGACCGTTACGTCTCCCCGGTTTGGGTTTTATCCCGCGCAGACGGTACCCGCGTCGTACCCGGCTCCTAATCCGGAGTTCTCGGCACCCATAGCTAAAAGTAACAGCAAAAATCAGCAGCCGCCGCCGCAGCCCCAAGTGGAGGCGCCACCGGTTTCGAATAGTGCTATTGCTAAAACGAATAGTCATCACGATGCTAAAGAGCTCCACATGTTTGTATGGAGCTCCAGCGCGTCCCCGGTTTCCGATGGCGGTGGGCTTCATGTTTTTGGCGGGAACGATTCCAGTGCGGCCGAGCAATCTGGACGGTCCGATCAGGGCGCCAAGGAGATAAGAATGATTGTTGCTGATCACCCCCAAAACGGAGAAAACAAAGCGGCAGTTCCGGAGAGTGCGCCGACGGCGGAGGGGTACGGCAGGGAAGCGTTTAGCTTCGGCGGGAGGGGCGGCGAGGGAGAGGAGGCTGAGAGGGAAAGAGCGGGACCCACAGGTCTTACTAAGCTGGGGTCAAACTCCACTACGGAGCTGCACCCAAAGGGCGGCGCACTTGGTGATCCTCATGAGTCAAGTGTCGGAAAGCAAATGCCTCCCGCGAGCGTTATGACCCGTCTGATCTTGATCATGGTGTGGCGGAAGCTTATCCGCAATCCCAACACGTATTCGAGCCTCATTGGTCTCATCTGGTCTCTCATCGCTTTCCGGTGGCATGTGGAGATGCCGAGAATAGTAGAAAAGTCCATTTCCATACTATCTGATGCTGGCCTTGGAATGGCTATGTTCAGCTTAGGTCTTTTTATGGCACTTCAACCCAAGATAATAGCATGTGGGAACTCAGTTGCCGGATTTGCAATGGCAGTAAGGTTCCTCACTGGCCCCGCCGTCATGGCTGCCGCCTCCATTGCTGTTGGCCTCCGTGGCACCCTCCTCCACTTAGCTATTGTCCAGGCTGCTCTTCCTCAAGGAATTGTTCCCTTTGTATTTGCCAAGGAGTACAATGTTCATCCAGCCATTCTTAGCACTGGGGTTATTTTCGGAATGTTGATAGCTTTACCAATTACTTTGGTCTACTACATTCTTCTGGGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000578 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009914 GO:0009921 GO:0009926 GO:0009942 GO:0009958 GO:0009966 GO:0009986 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0012506 GO:0015562 GO:0016020 GO:0016043 GO:0016049 GO:0016328 GO:0021700 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0030054 GO:0030154 GO:0031090 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048583 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0080161 GO:0090558 GO:0090627 GO:0098796 GO:0098797 GO:0099402 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

661

Amino Acids

71.61

Weight (kDa)

8.2

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 10 - 655 1.7e-126 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 403
AasI GACNNNNNNGTC 3 cut(s) 548, 904, 1383
AatII GACGTC 1 cut(s) 552
Acc36I ACCTGC 1 cut(s) 403
Acc65I GGTACC 1 cut(s) 940
AccB1I GGYRCC 8 cut(s) 43, 476, 692, 940, 979, 1039, 1214, 1811
AccBSI CCGCTC 2 cut(s) 893, 1373
AccI GTMKAC 2 cut(s) 540, 1959
AccII CGCG 7 cut(s) 140, 570, 849, 932, 947, 1127, 1487
AccIII TCCGGA 3 cut(s) 627, 967, 1279
AclWI GGATC 1 cut(s) 1442
AcoI YGGCCR 4 cut(s) 57, 376, 674, 1185
AcyI GRCGYC 7 cut(s) 477, 549, 588, 693, 731, 1040, 1215
AdeI CACNNNGTG 1 cut(s) 1445
AfaI GTAC 9 cut(s) 53, 374, 423, 443, 470, 942, 953, 1307, 1886
AfiI CCNNNNNNNGG 9 cut(s) 87, 395, 622, 917, 918, 1036, 1256, 1433, 1663
AflIII ACRYGT 2 cut(s) 1104, 1548
AgeI ACCGGT 1 cut(s) 1045
AgsI TTSAA 4 cut(s) 535, 751, 793, 1700
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 1 cut(s) 1836
Alw21I GWGCWC 4 cut(s) 119, 716, 1101, 1121
Alw26I GTCTC 6 cut(s) 503, 532, 575, 912, 1574, 1584
AlwI GGATC 1 cut(s) 1442
Ama87I CYCGRG 2 cut(s) 293, 578
Aor13HI TCCGGA 3 cut(s) 627, 967, 1279
AoxI GGCC 8 cut(s) 57, 259, 376, 674, 1185, 1654, 1765, 1801
ApeKI GCWGC 7 cut(s) 288, 399, 1012, 1024, 1420, 1781, 1841
AsiGI ACCGGT 1 cut(s) 1045
Asp700I GAANNNNTTC 1 cut(s) 98
Asp718I GGTACC 1 cut(s) 940
AspLEI GCGC 9 cut(s) 479, 695, 890, 934, 1042, 1127, 1217, 1291, 1439
AspS9I GGNCC 7 cut(s) 267, 625, 815, 851, 1203, 1376, 1766
AsuC2I CCSGG 6 cut(s) 389, 506, 799, 913, 956, 1133
AsuHPI GGTGA 4 cut(s) 268, 679, 1238, 1457
AsuII TTCGAA 2 cut(s) 876, 1052
AvaI CYCGRG 2 cut(s) 293, 578
AvaII GGWCC 6 cut(s) 267, 625, 815, 851, 1203, 1376
BaeI ACNNNNGTAYC 4 cut(s) 413, 446, 932, 965
BalI TGGCCA 1 cut(s) 59
BanI GGYRCC 8 cut(s) 43, 476, 692, 940, 979, 1039, 1214, 1811
BanII GRGCYC 3 cut(s) 716, 1101, 1121
Bbv12I GWGCWC 4 cut(s) 119, 716, 1101, 1121
BbvI GCAGC 7 cut(s) 300, 411, 1024, 1036, 1407, 1768, 1828
BccI CCATC 3 cut(s) 530, 769, 1136
BceAI ACGGC 9 cut(s) 51, 91, 134, 301, 391, 661, 1311, 1324, 1757
BcgI CGANNNNNNTGC 2 cut(s) 1163, 1197
BciT130I CCWGG 1 cut(s) 1838
BclI TGATCA 2 cut(s) 1243, 1512
BcnI CCSGG 6 cut(s) 389, 506, 799, 913, 956, 1133
BcoDI GTCTC 6 cut(s) 503, 532, 575, 912, 1574, 1584
BfmI CTRYAG 1 cut(s) 425
BfoI RGCGCY 4 cut(s) 480, 696, 1043, 1218
BfuAI ACCTGC 1 cut(s) 403
BglI GCCNNNNNGGC 2 cut(s) 1778, 1809
BglII AGATCT 1 cut(s) 96
Bme1390I CCNGG 7 cut(s) 389, 506, 799, 913, 956, 1133, 1838
Bme18I GGWCC 6 cut(s) 267, 625, 815, 851, 1203, 1376
BmeT110I CYCGRG 2 cut(s) 293, 578
BmgBI CACGTC 1 cut(s) 619
BmgT120I GGNCC 7 cut(s) 267, 625, 815, 851, 1203, 1376, 1766
BmrFI CCNGG 7 cut(s) 389, 506, 799, 913, 956, 1133, 1838
BmrI ACTGGG 1 cut(s) 1923
BmsI GCATC 6 cut(s) 132, 441, 650, 1078, 1599, 1639
BmuI ACTGGG 1 cut(s) 1923
BpmI CTGGAG 2 cut(s) 215, 1105
Bpu10I CCTNAGC 2 cut(s) 710, 1678
Bpu14I TTCGAA 2 cut(s) 876, 1052
BpuEI CTTGAG 1 cut(s) 1836
BpuMI CCSGG 6 cut(s) 389, 506, 799, 913, 956, 1133
BsaAI YACGTR 2 cut(s) 55, 1551
BsaBI GATNNNNATC 1 cut(s) 1511
BsaHI GRCGYC 7 cut(s) 477, 549, 588, 693, 731, 1040, 1215
BsaI GGTCTC 4 cut(s) 503, 575, 1574, 1584
BsaWI WCCGGW 7 cut(s) 84, 207, 627, 967, 1045, 1279, 1593
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 161, 191
Bsc4I CCNNNNNNNGG 9 cut(s) 87, 395, 622, 917, 918, 1036, 1256, 1433, 1663
Bse118I RCCGGY 2 cut(s) 689, 1045
Bse1I ACTGG 4 cut(s) 112, 1179, 1768, 1918
Bse3DI GCAATG 3 cut(s) 669, 1749, 1791
Bse8I GATNNNNATC 1 cut(s) 1511
BseAI TCCGGA 3 cut(s) 627, 967, 1279
BseBI CCWGG 1 cut(s) 1838
BseGI GGATG 2 cut(s) 306, 1894
BseJI GATNNNNATC 1 cut(s) 1511
BseLI CCNNNNNNNGG 9 cut(s) 87, 395, 622, 917, 918, 1036, 1256, 1433, 1663
BseMI GCAATG 3 cut(s) 669, 1749, 1791
BseMII CTCAG 4 cut(s) 701, 908, 1350, 1740
BseNI ACTGG 4 cut(s) 112, 1179, 1768, 1918
BseRI GAGGAG 5 cut(s) 440, 443, 706, 1367, 1808
BseX3I CGGCCG 2 cut(s) 674, 1185
BseXI GCAGC 7 cut(s) 300, 411, 1024, 1036, 1407, 1768, 1828
BseYI CCCAGC 1 cut(s) 1396
BsgI GTGCAG 2 cut(s) 431, 1406
Bsh1236I CGCG 7 cut(s) 140, 570, 849, 932, 947, 1127, 1487
Bsh1285I CGRYCG 3 cut(s) 568, 677, 1188
BshFI GGCC 8 cut(s) 59, 261, 378, 676, 1187, 1656, 1767, 1803
BshNI GGYRCC 8 cut(s) 43, 476, 692, 940, 979, 1039, 1214, 1811
BshTI ACCGGT 1 cut(s) 1045
BsiEI CGRYCG 3 cut(s) 568, 677, 1188
BsiHKAI GWGCWC 4 cut(s) 119, 716, 1101, 1121
BsiHKCI CYCGRG 2 cut(s) 293, 578
BslFI GGGAC 3 cut(s) 137, 1114, 1389
BslI CCNNNNNNNGG 9 cut(s) 87, 395, 622, 917, 918, 1036, 1256, 1433, 1663
BsmAI GTCTC 6 cut(s) 503, 532, 575, 912, 1574, 1584
BsmBI CGTCTC 2 cut(s) 532, 912
BsmFI GGGAC 3 cut(s) 137, 1114, 1389
BsnI GGCC 8 cut(s) 59, 261, 378, 676, 1187, 1656, 1767, 1803
Bso31I GGTCTC 4 cut(s) 503, 575, 1574, 1584
BsoBI CYCGRG 2 cut(s) 293, 578
Bsp119I TTCGAA 2 cut(s) 876, 1052
Bsp1286I GDGCHC 4 cut(s) 119, 716, 1101, 1121
Bsp13I TCCGGA 3 cut(s) 627, 967, 1279
Bsp143I GATC 7 cut(s) 63, 96, 1207, 1243, 1447, 1506, 1512
Bsp68I TCGCGA 2 cut(s) 140, 570
BspANI GGCC 8 cut(s) 59, 261, 378, 676, 1187, 1656, 1767, 1803
BspCNI CTCAG 4 cut(s) 702, 907, 1351, 1739
BspEI TCCGGA 3 cut(s) 627, 967, 1279
BspFNI CGCG 7 cut(s) 140, 570, 849, 932, 947, 1127, 1487
BspHI TCATGA 1 cut(s) 1453
BspMAI CTGCAG 1 cut(s) 429
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 1 cut(s) 1442
BspQI GCTCTTC 1 cut(s) 1851
BspT104I TTCGAA 2 cut(s) 876, 1052
BspT107I GGYRCC 8 cut(s) 43, 476, 692, 940, 979, 1039, 1214, 1811
BspTNI GGTCTC 4 cut(s) 503, 575, 1574, 1584
BsrBI CCGCTC 2 cut(s) 893, 1373
BsrDI GCAATG 3 cut(s) 669, 1749, 1791
BsrFI RCCGGY 2 cut(s) 689, 1045
BsrI ACTGG 4 cut(s) 112, 1179, 1768, 1918
BssAI RCCGGY 2 cut(s) 689, 1045
BssMI GATC 7 cut(s) 63, 96, 1207, 1243, 1447, 1506, 1512
BssNI GRCGYC 7 cut(s) 477, 549, 588, 693, 731, 1040, 1215
BssT1I CCWWGG 4 cut(s) 784, 1218, 1657, 1878
Bst2UI CCWGG 1 cut(s) 1838
Bst4CI ACNGT 5 cut(s) 473, 566, 901, 940, 1203
Bst6I CTCTTC 4 cut(s) 461, 467, 873, 1851
BstACI GRCGYC 7 cut(s) 477, 549, 588, 693, 731, 1040, 1215
BstBAI YACGTR 2 cut(s) 55, 1551
BstBI TTCGAA 2 cut(s) 876, 1052
BstC8I GCNNGC 2 cut(s) 1489, 1654
BstDEI CTNAG 9 cut(s) 67, 710, 894, 1359, 1392, 1678, 1726, 1825, 1907
BstDSI CCRYGG 3 cut(s) 472, 847, 1807
BstF5I GGATG 2 cut(s) 306, 1894
BstFNI CGCG 7 cut(s) 140, 570, 849, 932, 947, 1127, 1487
BstH2I RGCGCY 4 cut(s) 480, 696, 1043, 1218
BstHHI GCGC 9 cut(s) 479, 695, 890, 934, 1042, 1127, 1217, 1291, 1439
BstKTI GATC 7 cut(s) 66, 99, 1210, 1246, 1450, 1509, 1515
BstMAI GTCTC 6 cut(s) 503, 532, 575, 912, 1574, 1584
BstMBI GATC 7 cut(s) 63, 96, 1207, 1243, 1447, 1506, 1512
BstMCI CGRYCG 3 cut(s) 568, 677, 1188
BstNI CCWGG 1 cut(s) 1838
BstNSI RCATGY 3 cut(s) 1108, 1604, 1719
BstSCI CCNGG 7 cut(s) 387, 504, 797, 911, 954, 1131, 1836
BstSFI CTRYAG 1 cut(s) 425
BstUI CGCG 7 cut(s) 140, 570, 849, 932, 947, 1127, 1487
BstV1I GCAGC 7 cut(s) 300, 411, 1024, 1036, 1407, 1768, 1828
BstX2I RGATCY 1 cut(s) 96
BstYI RGATCY 1 cut(s) 96
BstZI CGGCCG 2 cut(s) 674, 1185
BsuRI GGCC 8 cut(s) 59, 261, 378, 676, 1187, 1656, 1767, 1803
BtgI CCRYGG 3 cut(s) 472, 847, 1807
BtgZI GCGATG 2 cut(s) 199, 1570
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 2 cut(s) 306, 1894
BtsI GCAGTG 1 cut(s) 434
BtsIMutI CAGTG 5 cut(s) 119, 434, 1186, 1761, 1911
BtuMI TCGCGA 2 cut(s) 140, 570
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 2 cut(s) 1489, 1654
CciI TCATGA 1 cut(s) 1453
CfoI GCGC 9 cut(s) 479, 695, 890, 934, 1042, 1127, 1217, 1291, 1439
Cfr10I RCCGGY 2 cut(s) 689, 1045
Cfr13I GGNCC 7 cut(s) 267, 625, 815, 851, 1203, 1376, 1766
Cfr42I CCGCGG 1 cut(s) 850
CpoI CGGWCCG 2 cut(s) 851, 1203
CseI GACGC 4 cut(s) 596, 739, 936, 1116
Csp6I GTAC 9 cut(s) 52, 373, 422, 442, 469, 941, 952, 1306, 1885
CspAI ACCGGT 1 cut(s) 1045
CspI CGGWCCG 2 cut(s) 851, 1203
CviQI GTAC 9 cut(s) 52, 373, 422, 442, 469, 941, 952, 1306, 1885
DdeI CTNAG 9 cut(s) 67, 710, 894, 1359, 1392, 1678, 1726, 1825, 1907
DinI GGCGCC 4 cut(s) 478, 694, 1041, 1216
DpnI GATC 7 cut(s) 65, 98, 1209, 1245, 1449, 1508, 1514
DpnII GATC 7 cut(s) 63, 96, 1207, 1243, 1447, 1506, 1512
DraIII CACNNNGTG 1 cut(s) 1445
DrdI GACNNNNNNGTC 3 cut(s) 548, 904, 1383
DseDI GACNNNNNNGTC 3 cut(s) 548, 904, 1383
EaeI YGGCCR 4 cut(s) 57, 376, 674, 1185
EagI CGGCCG 2 cut(s) 674, 1185
Eam1104I CTCTTC 4 cut(s) 461, 467, 873, 1851
EarI CTCTTC 4 cut(s) 461, 467, 873, 1851
EciI GGCGGA 2 cut(s) 1313, 1540
Ecl136II GAGCTC 3 cut(s) 714, 1099, 1119
EclXI CGGCCG 2 cut(s) 674, 1185
Eco130I CCWWGG 4 cut(s) 784, 1218, 1657, 1878
Eco24I GRGCYC 3 cut(s) 716, 1101, 1121
Eco31I GGTCTC 4 cut(s) 503, 575, 1574, 1584
Eco47I GGWCC 6 cut(s) 267, 625, 815, 851, 1203, 1376
Eco52I CGGCCG 2 cut(s) 674, 1185
Eco53kI GAGCTC 3 cut(s) 714, 1099, 1119
Eco88I CYCGRG 2 cut(s) 293, 578
EcoICRI GAGCTC 3 cut(s) 714, 1099, 1119
EcoO109I RGGNCCY 1 cut(s) 1376
EcoRII CCWGG 1 cut(s) 1836
EcoT14I CCWWGG 4 cut(s) 784, 1218, 1657, 1878
EcoT38I GRGCYC 3 cut(s) 716, 1101, 1121
EgeI GGCGCC 4 cut(s) 478, 694, 1041, 1216
EheI GGCGCC 4 cut(s) 478, 694, 1041, 1216
ErhI CCWWGG 4 cut(s) 784, 1218, 1657, 1878
Esp3I CGTCTC 2 cut(s) 532, 912
FaqI GGGAC 3 cut(s) 137, 1114, 1389
FbaI TGATCA 2 cut(s) 1243, 1512
FblI GTMKAC 2 cut(s) 540, 1959
FokI GGATG 2 cut(s) 313, 1881
FriOI GRGCYC 3 cut(s) 716, 1101, 1121
GlaI GCGC 9 cut(s) 478, 694, 889, 933, 1041, 1126, 1216, 1290, 1438
GsaI CCCAGC 1 cut(s) 1400
GsuI CTGGAG 2 cut(s) 215, 1105
HaeII RGCGCY 4 cut(s) 480, 696, 1043, 1218
HaeIII GGCC 8 cut(s) 59, 261, 378, 676, 1187, 1656, 1767, 1803
HgaI GACGC 4 cut(s) 596, 739, 936, 1116
HhaI GCGC 9 cut(s) 479, 695, 890, 934, 1042, 1127, 1217, 1291, 1439
Hin1I GRCGYC 7 cut(s) 477, 549, 588, 693, 731, 1040, 1215
Hin6I GCGC 9 cut(s) 477, 693, 888, 932, 1040, 1125, 1215, 1289, 1437
HinP1I GCGC 9 cut(s) 477, 693, 888, 932, 1040, 1125, 1215, 1289, 1437
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HindIII AAGCTT 2 cut(s) 482, 1529
HinfI GANTC 7 cut(s) 352, 392, 542, 780, 856, 1175, 1457
HphI GGTGA 4 cut(s) 268, 679, 1238, 1457
Hpy166II GTNNAC 3 cut(s) 444, 541, 1960
Hpy8I GTNNAC 3 cut(s) 444, 541, 1960
HpyAV CCTTC 2 cut(s) 175, 541
HpyCH4III ACNGT 5 cut(s) 473, 566, 901, 940, 1203
HpyCH4IV ACGT 5 cut(s) 54, 549, 618, 905, 1550
HpyCH4V TGCA 5 cut(s) 412, 427, 432, 1423, 1742
HpyF3I CTNAG 9 cut(s) 67, 710, 894, 1359, 1392, 1678, 1726, 1825, 1907
HpySE526I ACGT 5 cut(s) 54, 549, 618, 905, 1550
Hsp92I GRCGYC 7 cut(s) 477, 549, 588, 693, 731, 1040, 1215
HspAI GCGC 9 cut(s) 477, 693, 888, 932, 1040, 1125, 1215, 1289, 1437
KasI GGCGCC 4 cut(s) 476, 692, 1039, 1214
KflI GGGWCCC 1 cut(s) 1376
Kpn2I TCCGGA 3 cut(s) 627, 967, 1279
KpnI GGTACC 1 cut(s) 944
Ksp22I TGATCA 2 cut(s) 1243, 1512
KspI CCGCGG 1 cut(s) 850
Kzo9I GATC 7 cut(s) 63, 96, 1207, 1243, 1447, 1506, 1512
LguI GCTCTTC 1 cut(s) 1851
Lsp1109I GCAGC 7 cut(s) 300, 411, 1024, 1036, 1407, 1768, 1828
LweI GCATC 6 cut(s) 132, 441, 650, 1078, 1599, 1639
MaeII ACGT 5 cut(s) 54, 549, 618, 905, 1550
MaeIII GTNAC 3 cut(s) 651, 901, 995
MalI GATC 7 cut(s) 65, 98, 1209, 1245, 1449, 1508, 1514
MbiI CCGCTC 2 cut(s) 893, 1373
MboI GATC 7 cut(s) 63, 96, 1207, 1243, 1447, 1506, 1512
MboII GAAGA 7 cut(s) 91, 106, 448, 454, 890, 1838, 1961
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 4 cut(s) 119, 716, 1101, 1121
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 7 cut(s) 309, 745, 808, 871, 883, 1857, 1947
MluNI TGGCCA 1 cut(s) 59
Mly113I GGCGCC 4 cut(s) 477, 693, 1040, 1215
MlyI GAGTC 4 cut(s) 536, 789, 850, 1466
MmeI TCCRAC 4 cut(s) 570, 660, 878, 1447
Mox20I TGGCCA 1 cut(s) 59
MroI TCCGGA 3 cut(s) 627, 967, 1279
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 1 cut(s) 59
MseI TTAA 1 cut(s) 362
MslI CAYNNNNRTG 1 cut(s) 1086
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 3 cut(s) 49, 358, 849
MspR9I CCNGG 7 cut(s) 389, 506, 799, 913, 956, 1133, 1838
MvaI CCWGG 1 cut(s) 1838
MvnI CGCG 7 cut(s) 140, 570, 849, 932, 947, 1127, 1487
NarI GGCGCC 4 cut(s) 477, 693, 1040, 1215
NciI CCSGG 6 cut(s) 389, 506, 799, 913, 956, 1133
NdeII GATC 7 cut(s) 63, 96, 1207, 1243, 1447, 1506, 1512
NmeAIII GCCGAG 7 cut(s) 237, 652, 721, 759, 956, 1213, 1638
NmuCI GTSAC 1 cut(s) 651
NruI TCGCGA 2 cut(s) 140, 570
NspI RCATGY 3 cut(s) 1108, 1604, 1719
NspV TTCGAA 2 cut(s) 876, 1052
PaeR7I CTCGAG 2 cut(s) 293, 578
PagI TCATGA 1 cut(s) 1453
PaqCI CACCTGC 1 cut(s) 403
PciI ACATGT 1 cut(s) 1104
PciSI GCTCTTC 1 cut(s) 1851
PcsI WCGNNNNNNNCGW 6 cut(s) 291, 546, 558, 567, 819, 944
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 3 cut(s) 352, 392, 1175
PfoI TCCNGGA 2 cut(s) 387, 504
PinAI ACCGGT 1 cut(s) 1045
PleI GAGTC 4 cut(s) 536, 788, 850, 1465
PluTI GGCGCC 4 cut(s) 480, 696, 1043, 1218
PpsI GAGTC 4 cut(s) 536, 788, 850, 1465
Ppu21I YACGTR 2 cut(s) 55, 1551
PpuMI RGGWCCY 1 cut(s) 1376
PscI ACATGT 1 cut(s) 1104
Psp124BI GAGCTC 3 cut(s) 716, 1101, 1121
Psp5II RGGWCCY 1 cut(s) 1376
Psp6I CCWGG 1 cut(s) 1836
PspFI CCCAGC 1 cut(s) 1396
PspGI CCWGG 1 cut(s) 1836
PspPI GGNCC 7 cut(s) 267, 625, 815, 851, 1203, 1376, 1766
PspPPI RGGWCCY 1 cut(s) 1376
PspXI VCTCGAGB 1 cut(s) 293
PstI CTGCAG 1 cut(s) 429
PsuI RGATCY 1 cut(s) 96
RruI TCGCGA 2 cut(s) 140, 570
RsaI GTAC 9 cut(s) 53, 374, 423, 443, 470, 942, 953, 1307, 1886
RsaNI GTAC 9 cut(s) 52, 373, 422, 442, 469, 941, 952, 1306, 1885
RseI CAYNNNNRTG 1 cut(s) 1086
Rsr2I CGGWCCG 2 cut(s) 851, 1203
RsrII CGGWCCG 2 cut(s) 851, 1203
SacI GAGCTC 3 cut(s) 716, 1101, 1121
SacII CCGCGG 1 cut(s) 850
SalI GTCGAC 1 cut(s) 539
SapI GCTCTTC 1 cut(s) 1851
SaqAI TTAA 1 cut(s) 362
Sau3AI GATC 7 cut(s) 63, 96, 1207, 1243, 1447, 1506, 1512
Sau96I GGNCC 7 cut(s) 267, 625, 815, 851, 1203, 1376, 1766
SchI GAGTC 4 cut(s) 536, 789, 850, 1466
ScrFI CCNGG 7 cut(s) 389, 506, 799, 913, 956, 1133, 1838
SduI GDGCHC 4 cut(s) 119, 716, 1101, 1121
SfaNI GCATC 6 cut(s) 132, 441, 650, 1078, 1599, 1639
SfcI CTRYAG 1 cut(s) 425
SfoI GGCGCC 4 cut(s) 478, 694, 1041, 1216
Sfr274I CTCGAG 2 cut(s) 293, 578
Sfr303I CCGCGG 1 cut(s) 850
SfuI TTCGAA 2 cut(s) 876, 1052
SgrAI CRCCGGYG 1 cut(s) 689
SgrBI CCGCGG 1 cut(s) 850
SinI GGWCC 6 cut(s) 267, 625, 815, 851, 1203, 1376
SlaI CTCGAG 2 cut(s) 293, 578
SmiMI CAYNNNNRTG 1 cut(s) 1086
SmlI CTYRAG 3 cut(s) 293, 578, 1851
SmoI CTYRAG 3 cut(s) 293, 578, 1851
Sse9I AATT 7 cut(s) 309, 745, 808, 871, 883, 1857, 1947
SspDI GGCGCC 4 cut(s) 476, 692, 1039, 1214
SstI GAGCTC 3 cut(s) 716, 1101, 1121
StyD4I CCNGG 7 cut(s) 387, 504, 797, 911, 954, 1131, 1836
StyI CCWWGG 4 cut(s) 784, 1218, 1657, 1878
TaaI ACNGT 5 cut(s) 473, 566, 901, 940, 1203
TaiI ACGT 5 cut(s) 57, 552, 621, 908, 1553
TaqI TCGA 9 cut(s) 294, 465, 540, 561, 579, 680, 876, 1052, 1556
TaqII GACCGA 1 cut(s) 599
TasI AATT 7 cut(s) 309, 745, 808, 871, 883, 1857, 1947
TatI WGTACW 1 cut(s) 1884
TfiI GAWTC 3 cut(s) 352, 392, 1175
Tru1I TTAA 1 cut(s) 362
Tru9I TTAA 1 cut(s) 362
TscAI CASTG 5 cut(s) 119, 434, 1186, 1768, 1918
TseFI GTSAC 1 cut(s) 651
TseI GCWGC 7 cut(s) 288, 399, 1012, 1024, 1420, 1781, 1841
Tsp45I GTSAC 1 cut(s) 651
TspDTI ATGAA 5 cut(s) 163, 202, 215, 1145, 1883
TspGWI ACGGA 5 cut(s) 70, 621, 1272, 1430, 1796
TspRI CASTG 5 cut(s) 119, 434, 1186, 1768, 1918
VpaK11BI GGWCC 6 cut(s) 267, 625, 815, 851, 1203, 1376
XceI RCATGY 3 cut(s) 1108, 1604, 1719
XhoI CTCGAG 2 cut(s) 293, 578
XmiI GTMKAC 2 cut(s) 540, 1959
XmnI GAANNNNTTC 1 cut(s) 98
ZraI GACGTC 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.