Rh4BG167500

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002177
Physical Location & Seq
Forward (+)
30085572 .. 30085925
354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG167500.1

Sequence Viewer

Length: 354 bp
ATGGGAATCCCACTGCTAATCGCCATGTACGGCCACCGAATCCCGGAGTCCGGCAGCCTGATGGTCCAGGTCGTCGTGCTCCAGTGCATCATTTGGTACACTCTGTTACTCTTCCTCTTCGAGTACCGCGGCGCCAAGCTGCTAATAATGGAGCAATTCCCGGAGACCGCCGCCTCCATTGTCTCCTTCAAGGTTGACTCCGACGTCATGTTCCTCGACAACCGCGACTTCCTCAAAACTGACGCCGAGATCGGCGACGATGGCAAGCTCCACGTCAAGGTGAGGAAGTCCAACGCGTCTAGACGGTCGCTCTCGGCAATGACACCTCTCCCGTCCAACCTCACTGGAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000578 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009914 GO:0009921 GO:0009926 GO:0009942 GO:0009958 GO:0009966 GO:0009986 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0012506 GO:0015562 GO:0016020 GO:0016043 GO:0016049 GO:0016328 GO:0021700 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0030054 GO:0030154 GO:0031090 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048583 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0080161 GO:0090558 GO:0090627 GO:0098796 GO:0098797 GO:0099402 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

117

Amino Acids

13.06

Weight (kDa)

6.72

Isoelectric Point (pI)

21.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 1 - 96 7.7e-20 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
AatII GACGTC 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 131
AccII CGCG 3 cut(s) 129, 225, 296
AciI CCGC 5 cut(s) 127, 129, 168, 171, 223
AcoI YGGCCR 1 cut(s) 31
AcyI GRCGYC 3 cut(s) 132, 204, 243
AfaI GTAC 3 cut(s) 29, 98, 125
AfiI CCNNNNNNNGG 3 cut(s) 43, 50, 277
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 1 cut(s) 190
AjiI CACGTC 1 cut(s) 274
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 2 cut(s) 139, 268
AluI AGCT 2 cut(s) 139, 268
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 2 cut(s) 158, 187
AoxI GGCC 1 cut(s) 31
ApeKI GCWGC 2 cut(s) 54, 139
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 64
AsuC2I CCSGG 2 cut(s) 44, 161
AsuHPI GGTGA 1 cut(s) 292
AvaII GGWCC 1 cut(s) 64
BanI GGYRCC 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 66, 126
BccI CCATC 2 cut(s) 55, 254
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 68
BcnI CCSGG 2 cut(s) 44, 161
BcoDI GTCTC 2 cut(s) 158, 187
BfaI CTAG 1 cut(s) 300
BfoI RGCGCY 1 cut(s) 135
BisI GCNGC 4 cut(s) 55, 130, 140, 171
BlsI GCNGC 4 cut(s) 56, 131, 141, 172
Bme1390I CCNGG 3 cut(s) 44, 68, 161
Bme18I GGWCC 1 cut(s) 64
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 1 cut(s) 133
BmrFI CCNGG 3 cut(s) 44, 68, 161
BmsI GCATC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 65
BpuMI CCSGG 2 cut(s) 44, 161
BsaHI GRCGYC 3 cut(s) 132, 204, 243
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 127
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 50, 277
Bse1I ACTGG 2 cut(s) 82, 349
Bse3DI GCAATG 1 cut(s) 324
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 127
BseLI CCNNNNNNNGG 3 cut(s) 43, 50, 277
BseMI GCAATG 1 cut(s) 324
BseNI ACTGG 2 cut(s) 82, 349
BseXI GCAGC 2 cut(s) 66, 126
Bsh1236I CGCG 3 cut(s) 129, 225, 296
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 1 cut(s) 33
BshNI GGYRCC 1 cut(s) 131
BsiEI CGRYCG 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 3 cut(s) 44, 51, 161
BslI CCNNNNNNNGG 3 cut(s) 43, 50, 277
BsmAI GTCTC 2 cut(s) 158, 187
BsnI GGCC 1 cut(s) 33
Bso31I GGTCTC 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 1 cut(s) 249
BspACI CCGC 5 cut(s) 127, 129, 168, 171, 223
BspANI GGCC 1 cut(s) 33
BspFNI CGCG 3 cut(s) 129, 225, 296
BspLI GGNNCC 1 cut(s) 133
BspT107I GGYRCC 1 cut(s) 131
BspTNI GGTCTC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 324
BsrI ACTGG 2 cut(s) 82, 349
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 1 cut(s) 249
BssNI GRCGYC 3 cut(s) 132, 204, 243
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 1 cut(s) 306
Bst6I CTCTTC 2 cut(s) 116, 122
BstACI GRCGYC 3 cut(s) 132, 204, 243
BstC8I GCNNGC 1 cut(s) 266
BstDSI CCRYGG 1 cut(s) 127
BstFNI CGCG 3 cut(s) 129, 225, 296
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 1 cut(s) 252
BstMAI GTCTC 2 cut(s) 158, 187
BstMBI GATC 1 cut(s) 249
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstNI CCWGG 1 cut(s) 68
BstSCI CCNGG 3 cut(s) 42, 66, 159
BstUI CGCG 3 cut(s) 129, 225, 296
BstV1I GCAGC 2 cut(s) 66, 126
BsuRI GGCC 1 cut(s) 33
BtgI CCRYGG 1 cut(s) 127
BtrI CACGTC 1 cut(s) 274
BtsI GCAGTG 1 cut(s) 11
BtsIMutI CAGTG 3 cut(s) 11, 89, 342
Cac8I GCNNGC 1 cut(s) 266
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 64
Cfr42I CCGCGG 1 cut(s) 130
CseI GACGC 2 cut(s) 251, 285
Csp6I GTAC 3 cut(s) 28, 97, 124
CviAII CATG 2 cut(s) 25, 208
CviJI RGCY 4 cut(s) 33, 57, 139, 268
CviKI_1 RGCY 4 cut(s) 33, 57, 139, 268
CviQI GTAC 3 cut(s) 28, 97, 124
DinI GGCGCC 1 cut(s) 133
DpnI GATC 1 cut(s) 251
DpnII GATC 1 cut(s) 249
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
EaeI YGGCCR 1 cut(s) 31
Eam1104I CTCTTC 2 cut(s) 116, 122
EarI CTCTTC 2 cut(s) 116, 122
Eco31I GGTCTC 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 66
EgeI GGCGCC 1 cut(s) 133
EheI GGCGCC 1 cut(s) 133
FaeI CATG 2 cut(s) 28, 211
FaiI YATR 2 cut(s) 26, 209
FatI CATG 2 cut(s) 24, 207
Fnu4HI GCNGC 4 cut(s) 55, 130, 140, 171
Fsp4HI GCNGC 4 cut(s) 55, 130, 140, 171
FspBI CTAG 1 cut(s) 300
GlaI GCGC 1 cut(s) 133
GluI GCNGC 4 cut(s) 55, 130, 140, 171
GsuI CTGGAG 1 cut(s) 65
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 1 cut(s) 33
HapII CCGG 3 cut(s) 44, 51, 161
HgaI GACGC 2 cut(s) 251, 285
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 3 cut(s) 132, 204, 243
Hin1II CATG 2 cut(s) 28, 211
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 4 cut(s) 6, 39, 47, 197
HpaII CCGG 3 cut(s) 44, 51, 161
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 2 cut(s) 99, 196
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 1 cut(s) 300
Hpy8I GTNNAC 2 cut(s) 99, 196
Hpy99I CGWCG 3 cut(s) 77, 206, 260
HpyAV CCTTC 1 cut(s) 196
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4IV ACGT 2 cut(s) 204, 273
HpyCH4V TGCA 1 cut(s) 87
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpySE526I ACGT 2 cut(s) 204, 273
Hsp92I GRCGYC 3 cut(s) 132, 204, 243
Hsp92II CATG 2 cut(s) 28, 211
HspAI GCGC 1 cut(s) 132
KasI GGCGCC 1 cut(s) 131
KspI CCGCGG 1 cut(s) 130
Kzo9I GATC 1 cut(s) 249
LmnI GCTCC 4 cut(s) 84, 151, 273, 347
LpnPI CCDG 8 cut(s) 53, 57, 64, 71, 80, 95, 174, 330
Lsp1109I GCAGC 2 cut(s) 66, 126
LweI GCATC 1 cut(s) 96
MaeI CTAG 1 cut(s) 300
MaeII ACGT 2 cut(s) 204, 273
MaeIII GTNAC 1 cut(s) 105
MalI GATC 1 cut(s) 251
MboI GATC 1 cut(s) 249
MboII GAAGA 2 cut(s) 103, 109
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 1 cut(s) 155
MluI ACGCGT 1 cut(s) 294
Mly113I GGCGCC 1 cut(s) 132
MlyI GAGTC 2 cut(s) 56, 191
MmeI TCCRAC 2 cut(s) 225, 315
MnlI CCTC 7 cut(s) 125, 184, 224, 242, 276, 336, 350
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 3 cut(s) 44, 51, 161
MspR9I CCNGG 3 cut(s) 44, 68, 161
MvaI CCWGG 1 cut(s) 68
MvnI CGCG 3 cut(s) 129, 225, 296
MwoI GCNNNNNNNGC 1 cut(s) 261
NarI GGCGCC 1 cut(s) 132
NciI CCSGG 2 cut(s) 44, 161
NdeII GATC 1 cut(s) 249
NlaIII CATG 2 cut(s) 28, 211
NlaIV GGNNCC 1 cut(s) 133
NmeAIII GCCGAG 2 cut(s) 271, 293
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 2 cut(s) 6, 39
PfoI TCCNGGA 2 cut(s) 42, 159
PkrI GCNGC 4 cut(s) 56, 131, 141, 172
PleI GAGTC 2 cut(s) 55, 191
PluTI GGCGCC 1 cut(s) 135
PpsI GAGTC 2 cut(s) 55, 191
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 133
PspPI GGNCC 1 cut(s) 64
RsaI GTAC 3 cut(s) 29, 98, 125
RsaNI GTAC 3 cut(s) 28, 97, 124
SacII CCGCGG 1 cut(s) 130
SatI GCNGC 4 cut(s) 55, 130, 140, 171
Sau3AI GATC 1 cut(s) 249
Sau96I GGNCC 1 cut(s) 64
SchI GAGTC 2 cut(s) 56, 191
ScrFI CCNGG 3 cut(s) 44, 68, 161
SduI GDGCHC 1 cut(s) 81
SetI ASST 9 cut(s) 72, 141, 195, 207, 270, 276, 282, 328, 342
SfaNI GCATC 1 cut(s) 96
SfoI GGCGCC 1 cut(s) 133
Sfr303I CCGCGG 1 cut(s) 130
SgrBI CCGCGG 1 cut(s) 130
SinI GGWCC 1 cut(s) 64
Sse9I AATT 1 cut(s) 155
SsiI CCGC 5 cut(s) 127, 129, 168, 171, 223
SspDI GGCGCC 1 cut(s) 131
SspMI CTAG 1 cut(s) 300
StyD4I CCNGG 3 cut(s) 42, 66, 159
TaaI ACNGT 1 cut(s) 306
TaiI ACGT 2 cut(s) 207, 276
TaqI TCGA 2 cut(s) 120, 216
TasI AATT 1 cut(s) 155
TauI GCSGC 2 cut(s) 132, 173
TfiI GAWTC 2 cut(s) 6, 39
TscAI CASTG 3 cut(s) 18, 89, 349
TseI GCWGC 2 cut(s) 54, 139
TspRI CASTG 3 cut(s) 18, 89, 349
VpaK11BI GGWCC 1 cut(s) 64
XbaI TCTAGA 1 cut(s) 299
XspI CTAG 1 cut(s) 300
ZraI GACGTC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.