RLG00000023177

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002177
Physical Location & Seq
Reverse (-)
19489981 .. 19490688
708 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023177

Sequence Viewer

Length: 390 bp
ATGCTTCCCAATACTCTGGTCATGGGAATCCCGCTGTTGATCGCCATGTACGGCCACCGAATCCCGGAGTTCGGCAGCCTGATGGTCCAGGTCGTCGTGCTCCAGTGCATCATTTGGTGCACTCTGTTACTCTTCCTCTTCAAGTACAGCGGAGCCAAGCTGCTGATAATGGAGCAGTTCCCAGAGACCGCCGCCTCCATCGCCTCCTTCAAGGTTGACTCCGACGTCATGTCCCTCGACGACCGCTACTTCCTCAAAATTGACTCCGAGATCAGCGGCGACAGCAAGCTCCACGTCAAGGTGAAGAAGTCTAACGCGTCCAGACGCTTTTTAGCTGAATATTTAACGACTTCATGTTGCAATGTTAATCTCGGTTATGTTGAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000578 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009914 GO:0009921 GO:0009926 GO:0009942 GO:0009958 GO:0009966 GO:0009986 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0012506 GO:0015562 GO:0016020 GO:0016043 GO:0016049 GO:0016328 GO:0021700 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0030054 GO:0030154 GO:0031090 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048583 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0080161 GO:0090558 GO:0090627 GO:0098796 GO:0098797 GO:0099402 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

130

Amino Acids

14.52

Weight (kDa)

6.71

Isoelectric Point (pI)

30.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 2 - 96 6.5e-21 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
AatII GACGTC 1 cut(s) 228
AccII CGCG 1 cut(s) 317
AciI CCGC 6 cut(s) 32, 150, 189, 192, 244, 276
AcoI YGGCCR 1 cut(s) 52
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 2 cut(s) 50, 146
AfiI CCNNNNNNNGG 3 cut(s) 64, 71, 298
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 3 cut(s) 142, 211, 383
AhdI GACNNNNNGTC 1 cut(s) 229
AjiI CACGTC 1 cut(s) 295
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 3 cut(s) 160, 289, 335
AluI AGCT 3 cut(s) 160, 289, 335
Alw21I GWGCWC 2 cut(s) 102, 122
Alw26I GTCTC 1 cut(s) 179
Alw44I GTGCAC 1 cut(s) 118
AoxI GGCC 1 cut(s) 52
ApaLI GTGCAC 1 cut(s) 118
ApeKI GCWGC 2 cut(s) 75, 160
AspS9I GGNCC 1 cut(s) 85
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 313
AvaII GGWCC 1 cut(s) 85
BaeGI GKGCMC 1 cut(s) 122
Bbv12I GWGCWC 2 cut(s) 102, 122
BbvI GCAGC 2 cut(s) 87, 147
BccI CCATC 2 cut(s) 76, 206
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 1 cut(s) 89
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 179
BisI GCNGC 4 cut(s) 76, 161, 192, 277
BlsI GCNGC 4 cut(s) 77, 162, 193, 278
Bme1390I CCNGG 2 cut(s) 65, 89
Bme18I GGWCC 1 cut(s) 85
BmeRI GACNNNNNGTC 1 cut(s) 229
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 2 cut(s) 65, 89
BmsI GCATC 1 cut(s) 117
BpmI CTGGAG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 179
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 71, 298
Bse1I ACTGG 1 cut(s) 103
Bse3DI GCAATG 1 cut(s) 367
BseBI CCWGG 1 cut(s) 89
BseLI CCNNNNNNNGG 3 cut(s) 64, 71, 298
BseMI GCAATG 1 cut(s) 367
BseNI ACTGG 1 cut(s) 103
BseSI GKGCMC 1 cut(s) 122
BseXI GCAGC 2 cut(s) 87, 147
Bsh1236I CGCG 1 cut(s) 317
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 1 cut(s) 54
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 2 cut(s) 102, 122
BsiSI CCGG 1 cut(s) 65
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 3 cut(s) 64, 71, 298
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 1 cut(s) 54
Bso31I GGTCTC 1 cut(s) 179
Bsp1286I GDGCHC 2 cut(s) 102, 122
Bsp143I GATC 2 cut(s) 39, 270
BspACI CCGC 6 cut(s) 32, 150, 189, 192, 244, 276
BspANI GGCC 1 cut(s) 54
BspFNI CGCG 1 cut(s) 317
BspLI GGNNCC 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 367
BsrI ACTGG 1 cut(s) 103
BssMI GATC 2 cut(s) 39, 270
BssNI GRCGYC 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 89
Bst6I CTCTTC 2 cut(s) 137, 143
BstACI GRCGYC 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 287
BstFNI CGCG 1 cut(s) 317
BstKTI GATC 2 cut(s) 42, 273
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 2 cut(s) 39, 270
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 2 cut(s) 200, 282
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 2 cut(s) 63, 87
BstSLI GKGCMC 1 cut(s) 122
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 2 cut(s) 87, 147
BstXI CCANNNNNNTGG 1 cut(s) 16
BsuRI GGCC 1 cut(s) 54
BtgZI GCGATG 1 cut(s) 184
BtrI CACGTC 1 cut(s) 295
BtsIMutI CAGTG 1 cut(s) 110
Cac8I GCNNGC 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 85
CseI GACGC 2 cut(s) 306, 333
Csp6I GTAC 2 cut(s) 49, 145
CviAII CATG 4 cut(s) 22, 46, 229, 354
CviJI RGCY 6 cut(s) 54, 78, 155, 160, 289, 335
CviKI_1 RGCY 6 cut(s) 54, 78, 155, 160, 289, 335
CviQI GTAC 2 cut(s) 49, 145
DpnI GATC 2 cut(s) 41, 272
DpnII GATC 2 cut(s) 39, 270
DrdI GACNNNNNNGTC 1 cut(s) 224
DriI GACNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 52
Eam1104I CTCTTC 2 cut(s) 137, 143
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 2 cut(s) 137, 143
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 87
FaeI CATG 4 cut(s) 25, 49, 232, 357
FaiI YATR 5 cut(s) 23, 47, 230, 355, 378
FaqI GGGAC 1 cut(s) 217
FatI CATG 4 cut(s) 21, 45, 228, 353
FauI CCCGC 1 cut(s) 39
Fnu4HI GCNGC 4 cut(s) 76, 161, 192, 277
Fsp4HI GCNGC 4 cut(s) 76, 161, 192, 277
GluI GCNGC 4 cut(s) 76, 161, 192, 277
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 1 cut(s) 54
HapII CCGG 1 cut(s) 65
HgaI GACGC 2 cut(s) 306, 333
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 4 cut(s) 25, 49, 232, 357
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 4 cut(s) 27, 60, 218, 263
HpaII CCGG 1 cut(s) 65
HphI GGTGA 1 cut(s) 313
Hpy166II GTNNAC 2 cut(s) 120, 217
Hpy188I TCNGA 2 cut(s) 223, 268
Hpy188III TCNNGA 1 cut(s) 321
Hpy8I GTNNAC 2 cut(s) 120, 217
Hpy99I CGWCG 3 cut(s) 98, 227, 242
HpyAV CCTTC 1 cut(s) 217
HpyCH4IV ACGT 2 cut(s) 225, 294
HpyCH4V TGCA 3 cut(s) 108, 120, 360
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 282
HpySE526I ACGT 2 cut(s) 225, 294
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 4 cut(s) 25, 49, 232, 357
Kzo9I GATC 2 cut(s) 39, 270
LmnI GCTCC 4 cut(s) 105, 152, 172, 294
LpnPI CCDG 8 cut(s) 2, 74, 78, 92, 101, 116, 195, 334
Lsp1109I GCAGC 2 cut(s) 87, 147
LweI GCATC 1 cut(s) 117
MaeII ACGT 2 cut(s) 225, 294
MaeIII GTNAC 1 cut(s) 126
MalI GATC 2 cut(s) 41, 272
MboI GATC 2 cut(s) 39, 270
MboII GAAGA 3 cut(s) 124, 130, 316
MhlI GDGCHC 2 cut(s) 102, 122
MluCI AATT 1 cut(s) 258
MluI ACGCGT 1 cut(s) 315
MlyI GAGTC 2 cut(s) 212, 257
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 5 cut(s) 146, 205, 214, 245, 263
MseI TTAA 2 cut(s) 344, 366
MspA1I CMGCKG 3 cut(s) 34, 150, 276
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 65, 89
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 2 cut(s) 200, 282
NciI CCSGG 1 cut(s) 65
NdeII GATC 2 cut(s) 39, 270
NlaIII CATG 4 cut(s) 25, 49, 232, 357
NlaIV GGNNCC 1 cut(s) 154
PfeI GAWTC 2 cut(s) 27, 60
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 4 cut(s) 77, 162, 193, 278
PleI GAGTC 2 cut(s) 212, 257
PpsI GAGTC 2 cut(s) 212, 257
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 1 cut(s) 85
RsaI GTAC 2 cut(s) 50, 146
RsaNI GTAC 2 cut(s) 49, 145
SaqAI TTAA 2 cut(s) 344, 366
SatI GCNGC 4 cut(s) 76, 161, 192, 277
Sau3AI GATC 2 cut(s) 39, 270
Sau96I GGNCC 1 cut(s) 85
SchI GAGTC 2 cut(s) 212, 257
ScrFI CCNGG 2 cut(s) 65, 89
SduI GDGCHC 2 cut(s) 102, 122
SetI ASST 8 cut(s) 93, 162, 216, 228, 291, 297, 303, 337
SfaNI GCATC 1 cut(s) 117
SinI GGWCC 1 cut(s) 85
Sse9I AATT 1 cut(s) 258
SsiI CCGC 6 cut(s) 32, 150, 189, 192, 244, 276
SspI AATATT 1 cut(s) 341
StyD4I CCNGG 2 cut(s) 63, 87
TaiI ACGT 2 cut(s) 228, 297
TaqI TCGA 1 cut(s) 237
TasI AATT 1 cut(s) 258
TatI WGTACW 1 cut(s) 144
TauI GCSGC 2 cut(s) 194, 279
TfiI GAWTC 2 cut(s) 27, 60
Tru1I TTAA 2 cut(s) 344, 366
Tru9I TTAA 2 cut(s) 344, 366
TscAI CASTG 1 cut(s) 110
TseI GCWGC 2 cut(s) 75, 160
TspDTI ATGAA 1 cut(s) 342
TspRI CASTG 1 cut(s) 110
VneI GTGCAC 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 85
ZraI GACGTC 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.