RchiOBHm_Chr4g0441311

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002177
Physical Location & Seq
Reverse (-)
63179411 .. 63183670
4260 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40916

Sequence Viewer

Length: 1989 bp
ATGATTTCCTGGAACGACCTCTACAAGGTCTTGACGGCGGTGGTGCCTCTCTACGTGGCCATGATCTTAGCCTACGGCTCCGTGCGGTGGTGGAAGATCTTCTCCCCGGACCAATGCTCCGGCATCAACCGTTTCGTCGCAATCTTCGCAGTTCCTCTTCTCTCCTTCCACTTCATCTCCACCAACGACCCTTACAACATGAACTTCCGCTTCATCGCCGCCGACACTCTCCAGAAGATCATCATGCTCTTCGCCCTGGCCCTCTGGACCAACTTCACCCGAAACGGAAGCCTCGAGTGGATGATCACGATTTTCTCGCTCTCCACGCTTCCCAATACTCTGGTCATGGGAATCCCGCTGCTGATCGCCATGTACGGCCACCGAATCCCGGAGTCCGGCAGCCTCATGGTCCAGGTCGTCGTGCTCCAGTGCATCATCTGGTACACTCTGCTACTCTTCCTCTTCGAGTACCGCGGCGCCAAGCTGCTGATAATGGAGCAGTTCCCGGAGACCGCCGCCTCCATCGTCTCCTTCAAGGTCGACTCCGACGTCATGTCCCTCGACGGCCGCGACTTCCTCGAAACCGACGCCGAGATCGGCGACGACGGCAAGCTCCACGTCAAGGTGAGGAAGTCCAACGCGTCCAGACGGTCGCTCTCCGCAATGACGCCTCGGCCGTCCAACCTCACCGGAGCCGAGATTTACAGCCTCAGCTCTTCCCGGAATCCCACTCCCCGTGGCTCCAATTTCAACCCCTCCGATTTTTACTCCATGATGGGAGTCCAGGGCTTCAATACCCGCCACTCCAATTTCGGACCCGCCGACTTGTACTCGGTTCAGTCCTCCCGAGGTCCGACTCCCAGACCCTCCAATTTCGAAGAGAATTCTGCCCCAATGGCGCAGACGGTTACTTCTCCCCGGTTCGGGTTTTACCCGGCCCAGACTGTCCCCTCGTCGTACCCGGCTCCGAATCCGGAATTCTCCGCCCCCCTCACTAAAACAACCTCCAAAAATAACCAACCGCAACCCCAACCCCAACCGCCGCCGCAGCAGGTTAACTCCAACAACAGTAGCAAAGCGAATCACGACGCGAAAGAGCTCCACATGTTCGTTTGGAGCTCCAGCAATTCCCCTGTTTCCGAAGGCGGAGGGCTCCACGTCTTCGGCGGACCTGACTTCGCCGCGTCGGAGCAATCGGGGCGGTCCGATCAGGGCGCCAAGGAGATCAGGATGTTGGTTGCTGACCACCCCCAGAATGGAGAAACCAAAGTGGCGGTTGCGGAGAGTGACGGCGATCACTACCACCACGGCGGAGAAGCCTTCACGTTCACCGGAAAGGTTGATCAGGGAGAGGAGGAGAGAGAAAAGGAGGGGCCAACCGGACTTAACAAGCAGGGGTCGAGCTCCACTGCCGAGCTACACGGGAAAGGTGGAGGAGGAGGAGGAGTGGGGGTTGATCACGAGTCCAGCGGCGGAAAACAAATGCCTCCGGCGAGCGTTATGACGCGTCTGATCTTGATCATGGTTTGGCGGAAGCTTATCCGCAATCCCAACACGTACTCTAGCCTCATCGGTCTCATCTGGTCGCTCATCGCTTTCCGGTGGGGCGTTGCTATGCCAAAAATAATAGAGAAGTCGATCTCCATTCTGTCCGATGCTGGGCTAGGAATGGCTATGTTCAGTTTAGGTCTTTTTATGGCACTTCAACCAAAGATAATAGCATGTGGAAACTCGGTGGCCGGGTTTGCAATGGCCGTGAGATTCCTCACTGGCCCGGCTGTGATGGCTGCAGCCTCCATTGCTGTTGGGCTACGTGGCACCCTCCTCCACTTAGCTATTGTCCAGGCTGCACTTCCTCAAGGAATTGTTCCCTTTGTCTTTGCCAAGGAGTACAATGTTCATCCATCCATTCTGAGCACTGCGGTTATATTCGGGATGTTGATAGCATTACCGATTACGCTGGTTTACTATATCGTTCTCGGACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000578 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009914 GO:0009921 GO:0009926 GO:0009942 GO:0009958 GO:0009966 GO:0009986 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0012506 GO:0015562 GO:0016020 GO:0016043 GO:0016049 GO:0016328 GO:0021700 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0030054 GO:0030154 GO:0031090 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048583 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0080161 GO:0090558 GO:0090627 GO:0098796 GO:0098797 GO:0099402 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

662

Amino Acids

71.96

Weight (kDa)

7.77

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 10 - 657 1.6e-127 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 548
AatII GACGTC 1 cut(s) 552
Acc36I ACCTGC 1 cut(s) 1042
AccB1I GGYRCC 4 cut(s) 43, 476, 1214, 1817
AccI GTMKAC 1 cut(s) 540
AccII CGCG 6 cut(s) 474, 570, 641, 1091, 1184, 1507
AccIII TCCGGA 1 cut(s) 973
AcoI YGGCCR 6 cut(s) 57, 376, 565, 674, 1737, 1752
AcsI RAATTY 2 cut(s) 883, 977
AcyI GRCGYC 5 cut(s) 477, 549, 588, 668, 1215
AfaI GTAC 7 cut(s) 374, 443, 470, 830, 959, 1559, 1892
AfiI CCNNNNNNNGG 9 cut(s) 25, 87, 388, 395, 622, 923, 924, 1256, 1659
AflIII ACRYGT 4 cut(s) 639, 1104, 1505, 1554
AgsI TTSAA 4 cut(s) 535, 751, 793, 1706
AhdI GACNNNNNGTC 1 cut(s) 553
AjiI CACGTC 2 cut(s) 619, 1159
AjnI CCWGG 5 cut(s) 8, 255, 411, 783, 1842
AluBI AGCT 9 cut(s) 484, 613, 714, 1099, 1119, 1404, 1417, 1537, 1835
AluI AGCT 9 cut(s) 484, 613, 714, 1099, 1119, 1404, 1417, 1537, 1835
Alw21I GWGCWC 5 cut(s) 426, 1101, 1121, 1406, 1919
Alw26I GTCTC 3 cut(s) 503, 532, 1580
Ama87I CYCGRG 2 cut(s) 293, 846
Aor13HI TCCGGA 1 cut(s) 973
ApeKI GCWGC 7 cut(s) 358, 399, 484, 1048, 1787, 1790, 1847
ApoI RAATTY 2 cut(s) 883, 977
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 3 cut(s) 479, 901, 1217
AsuC2I CCSGG 9 cut(s) 107, 389, 506, 721, 919, 935, 962, 1741, 1775
AsuHPI GGTGA 4 cut(s) 268, 637, 679, 1321
AsuII TTCGAA 1 cut(s) 876
AvaI CYCGRG 2 cut(s) 293, 846
AvaII GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1169, 1203
BalI TGGCCA 1 cut(s) 59
BanI GGYRCC 4 cut(s) 43, 476, 1214, 1817
BanII GRGCYC 4 cut(s) 1101, 1121, 1155, 1406
BauI CACGAG 1 cut(s) 1460
BbsI GAAGAC 1 cut(s) 1153
Bbv12I GWGCWC 5 cut(s) 426, 1101, 1121, 1406, 1919
BbvCI CCTCAGC 1 cut(s) 710
BbvI GCAGC 7 cut(s) 345, 411, 471, 1060, 1774, 1802, 1834
BccI CCATC 4 cut(s) 530, 769, 1777, 1912
BceAI ACGGC 9 cut(s) 51, 91, 391, 580, 622, 661, 1306, 1324, 1739
BciT130I CCWGG 5 cut(s) 10, 257, 413, 785, 1844
BclI TGATCA 4 cut(s) 303, 1342, 1456, 1518
BcnI CCSGG 9 cut(s) 107, 389, 506, 721, 919, 935, 962, 1741, 1775
BcoDI GTCTC 3 cut(s) 503, 532, 1580
BfaI CTAG 2 cut(s) 1563, 1664
BfmI CTRYAG 1 cut(s) 1788
BfoI RGCGCY 2 cut(s) 480, 1218
BfuAI ACCTGC 1 cut(s) 1042
BglI GCCNNNNNGGC 1 cut(s) 896
BglII AGATCT 1 cut(s) 96
Bme18I GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1169, 1203
BmeRI GACNNNNNGTC 1 cut(s) 553
BmeT110I CYCGRG 2 cut(s) 293, 846
BmgBI CACGTC 2 cut(s) 619, 1159
BmsI GCATC 3 cut(s) 132, 441, 1645
BpiI GAAGAC 1 cut(s) 1153
BpmI CTGGAG 3 cut(s) 215, 410, 1105
Bpu10I CCTNAGC 1 cut(s) 710
Bpu14I TTCGAA 1 cut(s) 876
BpuEI CTTGAG 1 cut(s) 1842
BpuMI CCSGG 9 cut(s) 107, 389, 506, 721, 919, 935, 962, 1741, 1775
BsaAI YACGTR 3 cut(s) 55, 1557, 1814
BsaBI GATNNNNATC 1 cut(s) 1517
BsaHI GRCGYC 5 cut(s) 477, 549, 588, 668, 1215
BsaI GGTCTC 2 cut(s) 503, 1580
BsaWI WCCGGW 5 cut(s) 689, 973, 1331, 1379, 1599
BsaXI ACNNNNNCTCC 8 cut(s) 101, 131, 161, 191, 1431, 1437, 1461, 1467
Bsc4I CCNNNNNNNGG 9 cut(s) 25, 87, 388, 395, 622, 923, 924, 1256, 1659
Bse1I ACTGG 2 cut(s) 427, 1774
Bse3DI GCAATG 3 cut(s) 669, 1755, 1797
Bse8I GATNNNNATC 1 cut(s) 1517
BseAI TCCGGA 1 cut(s) 973
BseBI CCWGG 5 cut(s) 10, 257, 413, 785, 1844
BseGI GGATG 5 cut(s) 306, 1236, 1900, 1904, 1941
BseJI GATNNNNATC 1 cut(s) 1517
BseLI CCNNNNNNNGG 9 cut(s) 25, 87, 388, 395, 622, 923, 924, 1256, 1659
BseMI GCAATG 3 cut(s) 669, 1755, 1797
BseMII CTCAG 2 cut(s) 724, 1904
BseNI ACTGG 2 cut(s) 427, 1774
BseRI GAGGAG 7 cut(s) 1367, 1370, 1449, 1452, 1455, 1458, 1814
BseX3I CGGCCG 2 cut(s) 565, 674
BseXI GCAGC 7 cut(s) 345, 411, 471, 1060, 1774, 1802, 1834
BseYI CCCAGC 1 cut(s) 1658
BsgI GTGCAG 1 cut(s) 1833
Bsh1236I CGCG 6 cut(s) 474, 570, 641, 1091, 1184, 1507
Bsh1285I CGRYCG 3 cut(s) 568, 653, 677
BshNI GGYRCC 4 cut(s) 43, 476, 1214, 1817
BsiEI CGRYCG 3 cut(s) 568, 653, 677
BsiHKAI GWGCWC 5 cut(s) 426, 1101, 1121, 1406, 1919
BsiHKCI CYCGRG 2 cut(s) 293, 846
BslFI GGGAC 2 cut(s) 541, 932
BslI CCNNNNNNNGG 9 cut(s) 25, 87, 388, 395, 622, 923, 924, 1256, 1659
BsmAI GTCTC 3 cut(s) 503, 532, 1580
BsmBI CGTCTC 1 cut(s) 532
BsmFI GGGAC 2 cut(s) 541, 932
Bso31I GGTCTC 2 cut(s) 503, 1580
BsoBI CYCGRG 2 cut(s) 293, 846
Bsp119I TTCGAA 1 cut(s) 876
Bsp1286I GDGCHC 6 cut(s) 426, 1101, 1121, 1155, 1406, 1919
Bsp13I TCCGGA 1 cut(s) 973
BspCNI CTCAG 2 cut(s) 723, 1905
BspEI TCCGGA 1 cut(s) 973
BspFNI CGCG 6 cut(s) 474, 570, 641, 1091, 1184, 1507
BspMAI CTGCAG 1 cut(s) 1792
BspMI ACCTGC 1 cut(s) 1042
BspQI GCTCTTC 2 cut(s) 254, 721
BspT104I TTCGAA 1 cut(s) 876
BspT107I GGYRCC 4 cut(s) 43, 476, 1214, 1817
BspTNI GGTCTC 2 cut(s) 503, 1580
BsrDI GCAATG 3 cut(s) 669, 1755, 1797
BsrI ACTGG 2 cut(s) 427, 1774
BssNI GRCGYC 5 cut(s) 477, 549, 588, 668, 1215
BssSI CACGAG 1 cut(s) 1460
BssT1I CCWWGG 2 cut(s) 1218, 1884
Bst2BI CACGAG 1 cut(s) 1460
Bst2UI CCWGG 5 cut(s) 10, 257, 413, 785, 1844
Bst4CI ACNGT 6 cut(s) 131, 651, 907, 946, 1070, 1986
Bst6I CTCTTC 6 cut(s) 162, 254, 461, 467, 721, 873
BstACI GRCGYC 5 cut(s) 477, 549, 588, 668, 1215
BstBAI YACGTR 3 cut(s) 55, 1557, 1814
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 2 cut(s) 611, 1495
BstDEI CTNAG 4 cut(s) 67, 710, 1831, 1913
BstDSI CCRYGG 3 cut(s) 472, 736, 1306
BstENI CCTNNNNNAGG 1 cut(s) 23
BstF5I GGATG 5 cut(s) 306, 1236, 1900, 1904, 1941
BstFNI CGCG 6 cut(s) 474, 570, 641, 1091, 1184, 1507
BstH2I RGCGCY 2 cut(s) 480, 1218
BstHHI GCGC 3 cut(s) 479, 901, 1217
BstMAI GTCTC 3 cut(s) 503, 532, 1580
BstMCI CGRYCG 3 cut(s) 568, 653, 677
BstMWI GCNNNNNNNGC 9 cut(s) 146, 325, 606, 896, 1048, 1198, 1745, 1784, 1799
BstNI CCWGG 5 cut(s) 10, 257, 413, 785, 1844
BstNSI RCATGY 2 cut(s) 1108, 1725
BstSFI CTRYAG 1 cut(s) 1788
BstUI CGCG 6 cut(s) 474, 570, 641, 1091, 1184, 1507
BstV1I GCAGC 7 cut(s) 345, 411, 471, 1060, 1774, 1802, 1834
BstV2I GAAGAC 1 cut(s) 1153
BstX2I RGATCY 1 cut(s) 96
BstXI CCANNNNNNTGG 1 cut(s) 340
BstYI RGATCY 1 cut(s) 96
BstZI CGGCCG 2 cut(s) 565, 674
BtgI CCRYGG 3 cut(s) 472, 736, 1306
BtgZI GCGATG 2 cut(s) 199, 1576
BtrI CACGTC 2 cut(s) 619, 1159
BtsCI GGATG 5 cut(s) 306, 1236, 1900, 1904, 1941
BtsI GCAGTG 2 cut(s) 1407, 1917
BtsIMutI CAGTG 4 cut(s) 434, 1407, 1767, 1917
BveI ACCTGC 1 cut(s) 1042
Cac8I GCNNGC 2 cut(s) 611, 1495
CfoI GCGC 3 cut(s) 479, 901, 1217
Cfr42I CCGCGG 1 cut(s) 475
CpoI CGGWCCG 1 cut(s) 1203
CseI GACGC 7 cut(s) 596, 630, 676, 1097, 1173, 1496, 1513
Csp6I GTAC 7 cut(s) 373, 442, 469, 829, 958, 1558, 1891
CspI CGGWCCG 1 cut(s) 1203
CviQI GTAC 7 cut(s) 373, 442, 469, 829, 958, 1558, 1891
DdeI CTNAG 4 cut(s) 67, 710, 1831, 1913
DinI GGCGCC 2 cut(s) 478, 1216
DrdI GACNNNNNNGTC 1 cut(s) 548
DriI GACNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 1 cut(s) 548
EaeI YGGCCR 6 cut(s) 57, 376, 565, 674, 1737, 1752
EagI CGGCCG 2 cut(s) 565, 674
Eam1104I CTCTTC 6 cut(s) 162, 254, 461, 467, 721, 873
Eam1105I GACNNNNNGTC 1 cut(s) 553
EarI CTCTTC 6 cut(s) 162, 254, 461, 467, 721, 873
EciI GGCGGA 6 cut(s) 973, 1161, 1182, 1326, 1488, 1546
Ecl136II GAGCTC 3 cut(s) 1099, 1119, 1404
EclXI CGGCCG 2 cut(s) 565, 674
Eco130I CCWWGG 2 cut(s) 1218, 1884
Eco24I GRGCYC 4 cut(s) 1101, 1121, 1155, 1406
Eco31I GGTCTC 2 cut(s) 503, 1580
Eco47I GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1169, 1203
Eco52I CGGCCG 2 cut(s) 565, 674
Eco53kI GAGCTC 3 cut(s) 1099, 1119, 1404
Eco88I CYCGRG 2 cut(s) 293, 846
EcoICRI GAGCTC 3 cut(s) 1099, 1119, 1404
EcoNI CCTNNNNNAGG 1 cut(s) 23
EcoRI GAATTC 2 cut(s) 883, 977
EcoRII CCWGG 5 cut(s) 8, 255, 411, 783, 1842
EcoT14I CCWWGG 2 cut(s) 1218, 1884
EcoT38I GRGCYC 4 cut(s) 1101, 1121, 1155, 1406
EgeI GGCGCC 2 cut(s) 478, 1216
EheI GGCGCC 2 cut(s) 478, 1216
ErhI CCWWGG 2 cut(s) 1218, 1884
Esp3I CGTCTC 1 cut(s) 532
FaqI GGGAC 2 cut(s) 541, 932
FauI CCCGC 3 cut(s) 363, 806, 826
FbaI TGATCA 4 cut(s) 303, 1342, 1456, 1518
FblI GTMKAC 1 cut(s) 540
FokI GGATG 5 cut(s) 313, 1243, 1887, 1891, 1948
FriOI GRGCYC 4 cut(s) 1101, 1121, 1155, 1406
FspBI CTAG 2 cut(s) 1563, 1664
GlaI GCGC 3 cut(s) 478, 900, 1216
GsaI CCCAGC 1 cut(s) 1662
GsuI CTGGAG 3 cut(s) 215, 410, 1105
HaeII RGCGCY 2 cut(s) 480, 1218
HgaI GACGC 7 cut(s) 596, 630, 676, 1097, 1173, 1496, 1513
HhaI GCGC 3 cut(s) 479, 901, 1217
Hin1I GRCGYC 5 cut(s) 477, 549, 588, 668, 1215
Hin6I GCGC 3 cut(s) 477, 899, 1215
HinP1I GCGC 3 cut(s) 477, 899, 1215
HincII GTYRAC 2 cut(s) 541, 1057
HindII GTYRAC 2 cut(s) 541, 1057
HindIII AAGCTT 1 cut(s) 1535
HpaI GTTAAC 1 cut(s) 1057
HphI GGTGA 4 cut(s) 268, 637, 679, 1321
Hpy166II GTNNAC 5 cut(s) 444, 541, 1057, 1329, 1966
Hpy8I GTNNAC 5 cut(s) 444, 541, 1057, 1329, 1966
HpyAV CCTTC 4 cut(s) 175, 541, 1136, 1330
HpyCH4III ACNGT 6 cut(s) 131, 651, 907, 946, 1070, 1986
HpyCH4IV ACGT 7 cut(s) 54, 549, 618, 1158, 1325, 1556, 1813
HpyCH4V TGCA 4 cut(s) 432, 1748, 1790, 1850
HpyF10VI GCNNNNNNNGC 9 cut(s) 146, 325, 606, 896, 1048, 1198, 1745, 1784, 1799
HpyF3I CTNAG 4 cut(s) 67, 710, 1831, 1913
HpySE526I ACGT 7 cut(s) 54, 549, 618, 1158, 1325, 1556, 1813
Hsp92I GRCGYC 5 cut(s) 477, 549, 588, 668, 1215
HspAI GCGC 3 cut(s) 477, 899, 1215
KasI GGCGCC 2 cut(s) 476, 1214
Kpn2I TCCGGA 1 cut(s) 973
Ksp22I TGATCA 4 cut(s) 303, 1342, 1456, 1518
KspAI GTTAAC 1 cut(s) 1057
KspI CCGCGG 1 cut(s) 475
LguI GCTCTTC 2 cut(s) 254, 721
Lsp1109I GCAGC 7 cut(s) 345, 411, 471, 1060, 1774, 1802, 1834
LweI GCATC 3 cut(s) 132, 441, 1645
MaeI CTAG 2 cut(s) 1563, 1664
MaeII ACGT 7 cut(s) 54, 549, 618, 1158, 1325, 1556, 1813
MaeIII GTNAC 2 cut(s) 907, 1286
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 6 cut(s) 426, 1101, 1121, 1155, 1406, 1919
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 7 cut(s) 745, 808, 871, 883, 977, 1126, 1863
MluI ACGCGT 2 cut(s) 639, 1505
MluNI TGGCCA 1 cut(s) 59
Mly113I GGCGCC 2 cut(s) 477, 1215
MlyI GAGTC 5 cut(s) 401, 536, 789, 850, 1472
MmeI TCCRAC 6 cut(s) 570, 660, 705, 878, 1086, 1167
Mox20I TGGCCA 1 cut(s) 59
MroI TCCGGA 1 cut(s) 973
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 1 cut(s) 59
MseI TTAA 2 cut(s) 1056, 1386
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 3 cut(s) 358, 474, 1470
MvaI CCWGG 5 cut(s) 10, 257, 413, 785, 1844
MvnI CGCG 6 cut(s) 474, 570, 641, 1091, 1184, 1507
MwoI GCNNNNNNNGC 9 cut(s) 146, 325, 606, 896, 1048, 1198, 1745, 1784, 1799
NarI GGCGCC 2 cut(s) 477, 1215
NciI CCSGG 9 cut(s) 107, 389, 506, 721, 919, 935, 962, 1741, 1775
NmeAIII GCCGAG 4 cut(s) 616, 652, 721, 1438
NmuCI GTSAC 1 cut(s) 1286
NspI RCATGY 2 cut(s) 1108, 1725
NspV TTCGAA 1 cut(s) 876
PaeR7I CTCGAG 1 cut(s) 293
PciI ACATGT 1 cut(s) 1104
PciSI GCTCTTC 2 cut(s) 254, 721
PcsI WCGNNNNNNNCGW 5 cut(s) 291, 546, 567, 674, 819
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 6 cut(s) 351, 384, 724, 970, 1081, 1761
PfoI TCCNGGA 4 cut(s) 8, 387, 504, 719
PleI GAGTC 5 cut(s) 400, 536, 788, 850, 1471
PluTI GGCGCC 2 cut(s) 480, 1218
PpsI GAGTC 5 cut(s) 400, 536, 788, 850, 1471
Ppu21I YACGTR 3 cut(s) 55, 1557, 1814
PscI ACATGT 1 cut(s) 1104
Psp124BI GAGCTC 3 cut(s) 1101, 1121, 1406
Psp6I CCWGG 5 cut(s) 8, 255, 411, 783, 1842
PspFI CCCAGC 1 cut(s) 1658
PspGI CCWGG 5 cut(s) 8, 255, 411, 783, 1842
PspXI VCTCGAGB 1 cut(s) 293
PsrI GAACNNNNNNTAC 1 cut(s) 37
PstI CTGCAG 1 cut(s) 1792
PsuI RGATCY 1 cut(s) 96
RsaI GTAC 7 cut(s) 374, 443, 470, 830, 959, 1559, 1892
RsaNI GTAC 7 cut(s) 373, 442, 469, 829, 958, 1558, 1891
Rsr2I CGGWCCG 1 cut(s) 1203
RsrII CGGWCCG 1 cut(s) 1203
SacI GAGCTC 3 cut(s) 1101, 1121, 1406
SacII CCGCGG 1 cut(s) 475
SalI GTCGAC 1 cut(s) 539
SapI GCTCTTC 2 cut(s) 254, 721
SaqAI TTAA 2 cut(s) 1056, 1386
SchI GAGTC 5 cut(s) 401, 536, 789, 850, 1472
SduI GDGCHC 6 cut(s) 426, 1101, 1121, 1155, 1406, 1919
SfaNI GCATC 3 cut(s) 132, 441, 1645
SfcI CTRYAG 1 cut(s) 1788
SfoI GGCGCC 2 cut(s) 478, 1216
Sfr274I CTCGAG 1 cut(s) 293
Sfr303I CCGCGG 1 cut(s) 475
SfuI TTCGAA 1 cut(s) 876
SgrBI CCGCGG 1 cut(s) 475
SinI GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1169, 1203
SlaI CTCGAG 1 cut(s) 293
SmlI CTYRAG 2 cut(s) 293, 1857
SmoI CTYRAG 2 cut(s) 293, 1857
Sse9I AATT 7 cut(s) 745, 808, 871, 883, 977, 1126, 1863
SspDI GGCGCC 2 cut(s) 476, 1214
SspMI CTAG 2 cut(s) 1563, 1664
SstI GAGCTC 3 cut(s) 1101, 1121, 1406
StyI CCWWGG 2 cut(s) 1218, 1884
TaaI ACNGT 6 cut(s) 131, 651, 907, 946, 1070, 1986
TaiI ACGT 7 cut(s) 57, 552, 621, 1161, 1328, 1559, 1816
TaqI TCGA 8 cut(s) 294, 465, 540, 561, 579, 876, 1400, 1637
TaqII GACCGA 1 cut(s) 1562
TasI AATT 7 cut(s) 745, 808, 871, 883, 977, 1126, 1863
TatI WGTACW 2 cut(s) 828, 1890
TauI GCSGC 8 cut(s) 221, 477, 518, 570, 1045, 1048, 1184, 1473
TfiI GAWTC 6 cut(s) 351, 384, 724, 970, 1081, 1761
Tru1I TTAA 2 cut(s) 1056, 1386
Tru9I TTAA 2 cut(s) 1056, 1386
TscAI CASTG 4 cut(s) 434, 1414, 1774, 1924
TseFI GTSAC 1 cut(s) 1286
TseI GCWGC 7 cut(s) 358, 399, 484, 1048, 1787, 1790, 1847
Tsp45I GTSAC 1 cut(s) 1286
TspDTI ATGAA 4 cut(s) 163, 202, 215, 1889
TspGWI ACGGA 2 cut(s) 70, 300
TspRI CASTG 4 cut(s) 434, 1414, 1774, 1924
VpaK11BI GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1169, 1203
XagI CCTNNNNNAGG 1 cut(s) 23
XapI RAATTY 2 cut(s) 883, 977
XceI RCATGY 2 cut(s) 1108, 1725
XcmI CCANNNNNNNNNTGG 1 cut(s) 1253
XhoI CTCGAG 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 1563, 1664
ZraI GACGTC 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.