MD16G1027900.v1.1

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002177
Physical Location & Seq
Forward (+)
1969784 .. 1974751
4968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1027900.v1.1.491

Sequence Viewer

Length: 1911 bp
ATGATTTCGTGGCACGACCTTTACAACGTGTTGACGGCGGTGGTGCCGCTGTACGTGGCGATGATCTTAGCCTATGGCTCCGTCCGGTGGTGGAAGATCTTCTCCCCTGACCAGTGCTCCGGCATCAACAGCTTCGTGGCGATATTCGCTGTCCCCTTGCTCTCCTTCCACTTCATCTCCACCAACGACCCCTACACCATGAACTTCCGGTTCATCGCCGCCGACACCCTCCAGAAAATCATCATGCTCTTCGCCCTCGGCCTATGGACCAACTTCACCCGCAACGGCAGCCTAGAGTGGCTGATTACGATTTTCTCCCTCTCCACCCTCCCCAACACTCTCGTCATGGGGATTCCGCTGTTAATCGCCATGTACGGCAAAAAAATCCCGGAATCCGGCAGCCTCATGGTGCAGCTGGTGGTGGTCCAGTGCATCATCTGGTACACTCTCCTACTCTTCCTGTTCGAGTACCGCGGTGCCAAGCTCTTAATCATGGAGCAGTTTCCGGAGACCGCTGCCTCCATCGTCTCCTTTAAACTCGACTCCGACGTCGTTTCGCTCGACGGCAGGGATTTTTTGGAAACCGATGCCGAAATCGGTAACGACGGAAAGCTCCACGTCAAGGTCCGGAAGTCCAACGCATCGAGGCGGTCGCTATCTGCAATGACGCCCCGGCCGTCTAACCTCAGCAGCGCGGAGATTTACAGCCTGAGCTCCTCCCGAAATCCCACCCCGCGAGGCTCCAACTTTAACCCCTCCGATTTGTACTCCGTGATGGGGGTTCATGGGTTCAACAGCCGCCACTCCAACTTCGGACCCGCCGACTTGTATTCGGTTCAGTCCTCCCGCGGTCCGACTCCACGGCCCTCCAATTTCGAGGAGAATTGCGCGGCTCAGACCGTTATGACGGTACCCGCATCGTACCCGGCCCCGAATCCTGAATTCTCGGCGCCCACAGCTAAAAATAGCAACACAATTCAACAGCAGCAGCAGCCGCAAGTGGCAGCGCAACCGGTTTCGGACAGTGCTGCTGCTAAAACGAATAGTCATCTCGATGCTAAAGAGCTCCACATGTTCGTTTGGAGCTCCAGCGAGTCCCCTGTTTCTGAAGGCAGTGGGCTCCATGTTTTTGGTGGGACCGATTTCGGTGCTGCCGAGGAATCTGGACGGTCCGATCATGGCGCCAAGGAGATCAGAATGCTTGTTGCTGACCACCTCCAAAATGGAGAAAACAAAGTTCCGGAGAGTGCGCCAACGGCGGAGGGGTACGGCGGGGAAGCGTTGAGATGTGGCTGGAGGGGTGGCGAGGGAGAGGAGGCTGAAGGGGAAAAAGAGGGAACCACAGGGCTTAATAAGCTGGCGTCCAGCTCCACTGCAGAGCTGCACGCAAGGGGCGGAAGGCAAATGCCGCCGGCGAGCGTTATCACCCGTCTGATCTTGATCATGGTTTGGCGGAAGCTTATCCGTAACCCCAACACGTATTCGAGCCTCATTGGTCTCATCTGGGCTCTCATCGCTTTCCGGTGGCATGTGGGGATGCCAAAAATAGTAGAAAAGTCAATTTCCATACTGTCTGATGCTGGACTTGGAATGGCTATGTTCAGCTTAGGTCTTTTCATGGCACTTCAACCCAGGATAATAGCATGTGGGAACTCAGTTGGCGGATATGCAATGGCAGTAAGGTTCCTCACTGGCCCCGCCGTTATGGCTGCCGCCTCCATTGCTGTTGGCCTCCGGGGCACCCTCCTCCACTTAGCGATTGTCCAGGCTGCTCTTCCTCAAGGACTTGTTCCCTTTGTCTTTGCCAAGGAGTACAATGTTCATCCAGCCATTCTTAGCACTGCGGTTATTTTCGGAATGTTGATAGCGTTACCTATTACATTGGTCTACTATATTCTTCTGGGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000578 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009914 GO:0009921 GO:0009926 GO:0009942 GO:0009958 GO:0009966 GO:0009986 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0012506 GO:0015562 GO:0016020 GO:0016043 GO:0016049 GO:0016328 GO:0021700 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0030054 GO:0030154 GO:0031090 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048583 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0080161 GO:0090558 GO:0090627 GO:0098796 GO:0098797 GO:0099402 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

637

Amino Acids

69.02

Weight (kDa)

6.93

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 10 - 631 4.7e-123 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 548
AatII GACGTC 1 cut(s) 552
Acc65I GGTACC 1 cut(s) 910
AccB1I GGYRCC 6 cut(s) 43, 476, 910, 949, 1181, 1739
AccI GTMKAC 1 cut(s) 1887
AccII CGCG 5 cut(s) 474, 695, 736, 849, 890
AccIII TCCGGA 3 cut(s) 505, 627, 1240
AcoI YGGCCR 1 cut(s) 674
AcsI RAATTY 1 cut(s) 941
AcuI CTGAAG 2 cut(s) 1128, 1341
AcyI GRCGYC 5 cut(s) 549, 668, 950, 1182, 1361
AfaI GTAC 9 cut(s) 53, 374, 443, 470, 767, 912, 923, 1268, 1814
AfiI CCNNNNNNNGG 4 cut(s) 87, 395, 622, 777
AflIII ACRYGT 3 cut(s) 27, 1071, 1476
AgeI ACCGGT 1 cut(s) 1012
AgsI TTSAA 3 cut(s) 793, 980, 1628
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 2 cut(s) 1631, 1764
Alw21I GWGCWC 4 cut(s) 119, 716, 1068, 1088
Alw26I GTCTC 3 cut(s) 503, 532, 1502
Aor13HI TCCGGA 3 cut(s) 505, 627, 1240
AoxI GGCC 6 cut(s) 259, 674, 863, 927, 1693, 1729
ApoI RAATTY 1 cut(s) 941
AsiGI ACCGGT 1 cut(s) 1012
Asp700I GAANNNNTTC 1 cut(s) 98
Asp718I GGTACC 1 cut(s) 910
AspLEI GCGC 6 cut(s) 695, 890, 952, 1009, 1184, 1252
AsuC2I CCSGG 4 cut(s) 389, 673, 926, 1736
AsuHPI GGTGA 2 cut(s) 268, 1417
AvaII GGWCC 7 cut(s) 267, 424, 625, 815, 851, 1137, 1170
BaeGI GKGCMC 1 cut(s) 1742
BanI GGYRCC 6 cut(s) 43, 476, 910, 949, 1181, 1739
BanII GRGCYC 5 cut(s) 716, 1068, 1088, 1122, 1510
Bbv12I GWGCWC 4 cut(s) 119, 716, 1068, 1088
BbvCI CCTCAGC 1 cut(s) 686
BccI CCATC 2 cut(s) 530, 769
BceAI ACGGC 9 cut(s) 51, 301, 391, 580, 661, 878, 1272, 1285, 1685
BcgI CGANNNNNNTGC 2 cut(s) 1130, 1164
BciT130I CCWGG 2 cut(s) 1633, 1766
BclI TGATCA 1 cut(s) 1440
BcnI CCSGG 4 cut(s) 389, 673, 926, 1736
BcoDI GTCTC 3 cut(s) 503, 532, 1502
BfaI CTAG 1 cut(s) 293
BfmI CTRYAG 1 cut(s) 1374
BfoI RGCGCY 2 cut(s) 953, 1185
BglI GCCNNNNNGGC 2 cut(s) 1706, 1737
BglII AGATCT 1 cut(s) 96
Bme1390I CCNGG 6 cut(s) 389, 673, 926, 1633, 1736, 1766
Bme18I GGWCC 7 cut(s) 267, 424, 625, 815, 851, 1137, 1170
BmgBI CACGTC 1 cut(s) 619
BmrFI CCNGG 6 cut(s) 389, 673, 926, 1633, 1736, 1766
BmsI GCATC 8 cut(s) 132, 441, 577, 650, 926, 1045, 1527, 1567
BpmI CTGGAG 3 cut(s) 215, 1072, 1315
Bpu10I CCTNAGC 3 cut(s) 686, 710, 1606
BpuEI CTTGAG 1 cut(s) 1764
BpuMI CCSGG 4 cut(s) 389, 673, 926, 1736
BsaAI YACGTR 2 cut(s) 55, 1479
BsaBI GATNNNNATC 1 cut(s) 1439
BsaHI GRCGYC 5 cut(s) 549, 668, 950, 1182, 1361
BsaI GGTCTC 2 cut(s) 503, 1502
BsaWI WCCGGW 7 cut(s) 84, 207, 505, 627, 1012, 1240, 1521
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 161, 191
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 395, 622, 777
Bse118I RCCGGY 2 cut(s) 1012, 1411
Bse1I ACTGG 3 cut(s) 112, 427, 1696
Bse3DI GCAATG 3 cut(s) 669, 1677, 1719
Bse8I GATNNNNATC 1 cut(s) 1439
BseAI TCCGGA 3 cut(s) 505, 627, 1240
BseBI CCWGG 2 cut(s) 1633, 1766
BseGI GGATG 2 cut(s) 1542, 1822
BseJI GATNNNNATC 1 cut(s) 1439
BseLI CCNNNNNNNGG 4 cut(s) 87, 395, 622, 777
BseMI GCAATG 3 cut(s) 669, 1677, 1719
BseMII CTCAG 4 cut(s) 700, 701, 908, 1668
BseNI ACTGG 3 cut(s) 112, 427, 1696
BseRI GAGGAG 4 cut(s) 706, 893, 1328, 1736
BseSI GKGCMC 1 cut(s) 1742
BseX3I CGGCCG 1 cut(s) 674
BsgI GTGCAG 2 cut(s) 431, 1367
Bsh1236I CGCG 5 cut(s) 474, 695, 736, 849, 890
Bsh1285I CGRYCG 2 cut(s) 653, 677
BshFI GGCC 6 cut(s) 261, 676, 865, 929, 1695, 1731
BshNI GGYRCC 6 cut(s) 43, 476, 910, 949, 1181, 1739
BshTI ACCGGT 1 cut(s) 1012
BsiEI CGRYCG 2 cut(s) 653, 677
BsiHKAI GWGCWC 4 cut(s) 119, 716, 1068, 1088
BslFI GGGAC 3 cut(s) 137, 1081, 1150
BslI CCNNNNNNNGG 4 cut(s) 87, 395, 622, 777
BsmAI GTCTC 3 cut(s) 503, 532, 1502
BsmBI CGTCTC 1 cut(s) 532
BsmFI GGGAC 3 cut(s) 137, 1081, 1150
BsmI GAATGC 1 cut(s) 1203
BsnI GGCC 6 cut(s) 261, 676, 865, 929, 1695, 1731
Bso31I GGTCTC 2 cut(s) 503, 1502
Bsp1286I GDGCHC 7 cut(s) 119, 716, 1068, 1088, 1122, 1510, 1742
Bsp13I TCCGGA 3 cut(s) 505, 627, 1240
Bsp143I GATC 6 cut(s) 63, 96, 1174, 1191, 1434, 1440
BspANI GGCC 6 cut(s) 261, 676, 865, 929, 1695, 1731
BspCNI CTCAG 4 cut(s) 699, 702, 907, 1667
BspEI TCCGGA 3 cut(s) 505, 627, 1240
BspFNI CGCG 5 cut(s) 474, 695, 736, 849, 890
BspMAI CTGCAG 1 cut(s) 1378
BspQI GCTCTTC 2 cut(s) 254, 1779
BspT107I GGYRCC 6 cut(s) 43, 476, 910, 949, 1181, 1739
BspTNI GGTCTC 2 cut(s) 503, 1502
BsrDI GCAATG 3 cut(s) 669, 1677, 1719
BsrFI RCCGGY 2 cut(s) 1012, 1411
BsrI ACTGG 3 cut(s) 112, 427, 1696
BssAI RCCGGY 2 cut(s) 1012, 1411
BssMI GATC 6 cut(s) 63, 96, 1174, 1191, 1434, 1440
BssNI GRCGYC 5 cut(s) 549, 668, 950, 1182, 1361
BssT1I CCWWGG 2 cut(s) 1185, 1806
Bst2UI CCWGG 2 cut(s) 1633, 1766
Bst4CI ACNGT 5 cut(s) 901, 910, 1025, 1170, 1572
Bst6I CTCTTC 3 cut(s) 254, 461, 1779
BstACI GRCGYC 5 cut(s) 549, 668, 950, 1182, 1361
BstBAI YACGTR 2 cut(s) 55, 1479
BstC8I GCNNGC 4 cut(s) 1359, 1386, 1413, 1417
BstDEI CTNAG 8 cut(s) 67, 686, 710, 894, 1606, 1654, 1753, 1835
BstDSI CCRYGG 3 cut(s) 472, 847, 860
BstF5I GGATG 2 cut(s) 1542, 1822
BstFNI CGCG 5 cut(s) 474, 695, 736, 849, 890
BstH2I RGCGCY 2 cut(s) 953, 1185
BstHHI GCGC 6 cut(s) 695, 890, 952, 1009, 1184, 1252
BstKTI GATC 6 cut(s) 66, 99, 1177, 1194, 1437, 1443
BstMAI GTCTC 3 cut(s) 503, 532, 1502
BstMBI GATC 6 cut(s) 63, 96, 1174, 1191, 1434, 1440
BstMCI CGRYCG 2 cut(s) 653, 677
BstNI CCWGG 2 cut(s) 1633, 1766
BstNSI RCATGY 3 cut(s) 1075, 1532, 1647
BstSCI CCNGG 6 cut(s) 387, 671, 924, 1631, 1734, 1764
BstSFI CTRYAG 1 cut(s) 1374
BstSLI GKGCMC 1 cut(s) 1742
BstUI CGCG 5 cut(s) 474, 695, 736, 849, 890
BstX2I RGATCY 1 cut(s) 96
BstXI CCANNNNNNTGG 1 cut(s) 1130
BstYI RGATCY 1 cut(s) 96
BstZI CGGCCG 1 cut(s) 674
BsuRI GGCC 6 cut(s) 261, 676, 865, 929, 1695, 1731
BtgI CCRYGG 3 cut(s) 472, 847, 860
BtgZI GCGATG 3 cut(s) 74, 199, 1498
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 2 cut(s) 1542, 1822
BtsI GCAGTG 3 cut(s) 1120, 1371, 1839
BtsIMutI CAGTG 7 cut(s) 119, 434, 1030, 1120, 1371, 1689, 1839
Cac8I GCNNGC 4 cut(s) 1359, 1386, 1413, 1417
CfoI GCGC 6 cut(s) 695, 890, 952, 1009, 1184, 1252
Cfr10I RCCGGY 2 cut(s) 1012, 1411
Cfr42I CCGCGG 2 cut(s) 475, 850
CpoI CGGWCCG 2 cut(s) 851, 1170
CseI GACGC 2 cut(s) 676, 1350
Csp6I GTAC 9 cut(s) 52, 373, 442, 469, 766, 911, 922, 1267, 1813
CspAI ACCGGT 1 cut(s) 1012
CspI CGGWCCG 2 cut(s) 851, 1170
CviQI GTAC 9 cut(s) 52, 373, 442, 469, 766, 911, 922, 1267, 1813
DdeI CTNAG 8 cut(s) 67, 686, 710, 894, 1606, 1654, 1753, 1835
DinI GGCGCC 2 cut(s) 951, 1183
DpnI GATC 6 cut(s) 65, 98, 1176, 1193, 1436, 1442
DpnII GATC 6 cut(s) 63, 96, 1174, 1191, 1434, 1440
DraI TTTAAA 1 cut(s) 535
DrdI GACNNNNNNGTC 1 cut(s) 548
DseDI GACNNNNNNGTC 1 cut(s) 548
EaeI YGGCCR 1 cut(s) 674
EagI CGGCCG 1 cut(s) 674
Eam1104I CTCTTC 3 cut(s) 254, 461, 1779
EarI CTCTTC 3 cut(s) 254, 461, 1779
EciI GGCGGA 4 cut(s) 1274, 1410, 1468, 1677
Ecl136II GAGCTC 3 cut(s) 714, 1066, 1086
EclXI CGGCCG 1 cut(s) 674
Eco130I CCWWGG 2 cut(s) 1185, 1806
Eco24I GRGCYC 5 cut(s) 716, 1068, 1088, 1122, 1510
Eco31I GGTCTC 2 cut(s) 503, 1502
Eco47I GGWCC 7 cut(s) 267, 424, 625, 815, 851, 1137, 1170
Eco52I CGGCCG 1 cut(s) 674
Eco53kI GAGCTC 3 cut(s) 714, 1066, 1086
Eco57I CTGAAG 2 cut(s) 1128, 1341
EcoICRI GAGCTC 3 cut(s) 714, 1066, 1086
EcoRI GAATTC 1 cut(s) 941
EcoRII CCWGG 2 cut(s) 1631, 1764
EcoT14I CCWWGG 2 cut(s) 1185, 1806
EcoT38I GRGCYC 5 cut(s) 716, 1068, 1088, 1122, 1510
EgeI GGCGCC 2 cut(s) 951, 1183
EheI GGCGCC 2 cut(s) 951, 1183
ErhI CCWWGG 2 cut(s) 1185, 1806
Esp3I CGTCTC 1 cut(s) 532
FaqI GGGAC 3 cut(s) 137, 1081, 1150
FauI CCCGC 7 cut(s) 287, 741, 826, 854, 922, 1265, 1705
FbaI TGATCA 1 cut(s) 1440
FblI GTMKAC 1 cut(s) 1887
FokI GGATG 2 cut(s) 1549, 1809
FriOI GRGCYC 5 cut(s) 716, 1068, 1088, 1122, 1510
FspBI CTAG 1 cut(s) 293
GlaI GCGC 6 cut(s) 694, 889, 951, 1008, 1183, 1251
GsuI CTGGAG 3 cut(s) 215, 1072, 1315
HaeII RGCGCY 2 cut(s) 953, 1185
HaeIII GGCC 6 cut(s) 261, 676, 865, 929, 1695, 1731
HgaI GACGC 2 cut(s) 676, 1350
HhaI GCGC 6 cut(s) 695, 890, 952, 1009, 1184, 1252
Hin1I GRCGYC 5 cut(s) 549, 668, 950, 1182, 1361
Hin6I GCGC 6 cut(s) 693, 888, 950, 1007, 1182, 1250
HinP1I GCGC 6 cut(s) 693, 888, 950, 1007, 1182, 1250
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HindIII AAGCTT 1 cut(s) 1457
HinfI GANTC 7 cut(s) 352, 392, 542, 856, 934, 1094, 1160
HphI GGTGA 2 cut(s) 268, 1417
Hpy166II GTNNAC 3 cut(s) 33, 444, 1888
Hpy188III TCNNGA 9 cut(s) 232, 506, 628, 720, 938, 1052, 1164, 1241, 1438
Hpy8I GTNNAC 3 cut(s) 33, 444, 1888
Hpy99I CGWCG 4 cut(s) 551, 554, 566, 608
HpyAV CCTTC 4 cut(s) 175, 1103, 1316, 1392
HpyCH4III ACNGT 5 cut(s) 901, 910, 1025, 1170, 1572
HpyCH4IV ACGT 5 cut(s) 27, 54, 549, 618, 1478
HpyCH4V TGCA 6 cut(s) 412, 432, 662, 1376, 1384, 1670
HpyF3I CTNAG 8 cut(s) 67, 686, 710, 894, 1606, 1654, 1753, 1835
HpySE526I ACGT 5 cut(s) 27, 54, 549, 618, 1478
Hsp92I GRCGYC 5 cut(s) 549, 668, 950, 1182, 1361
HspAI GCGC 6 cut(s) 693, 888, 950, 1007, 1182, 1250
KasI GGCGCC 2 cut(s) 949, 1181
Kpn2I TCCGGA 3 cut(s) 505, 627, 1240
KpnI GGTACC 1 cut(s) 914
KroI GCCGGC 1 cut(s) 1411
KroNI GCCGGC 1 cut(s) 1413
Ksp22I TGATCA 1 cut(s) 1440
KspI CCGCGG 2 cut(s) 475, 850
Kzo9I GATC 6 cut(s) 63, 96, 1174, 1191, 1434, 1440
LguI GCTCTTC 2 cut(s) 254, 1779
LweI GCATC 8 cut(s) 132, 441, 577, 650, 926, 1045, 1527, 1567
MaeI CTAG 1 cut(s) 293
MaeII ACGT 5 cut(s) 27, 54, 549, 618, 1478
MaeIII GTNAC 3 cut(s) 599, 1466, 1869
MalI GATC 6 cut(s) 65, 98, 1176, 1193, 1436, 1442
MboI GATC 6 cut(s) 63, 96, 1174, 1191, 1434, 1440
MboII GAAGA 6 cut(s) 91, 106, 241, 448, 1766, 1889
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 7 cut(s) 119, 716, 1068, 1088, 1122, 1510, 1742
MluCI AATT 5 cut(s) 871, 883, 941, 975, 1560
Mly113I GGCGCC 2 cut(s) 950, 1182
MlyI GAGTC 3 cut(s) 536, 850, 1103
MmeI TCCRAC 5 cut(s) 570, 660, 768, 831, 878
MreI CGCCGGCG 1 cut(s) 1411
MroI TCCGGA 3 cut(s) 505, 627, 1240
MroNI GCCGGC 1 cut(s) 1411
MroXI GAANNNNTTC 1 cut(s) 98
MseI TTAA 5 cut(s) 362, 488, 534, 750, 1350
MslI CAYNNNNRTG 1 cut(s) 1053
MspA1I CMGCKG 6 cut(s) 49, 358, 415, 474, 515, 849
MspR9I CCNGG 6 cut(s) 389, 673, 926, 1633, 1736, 1766
Mva1269I GAATGC 1 cut(s) 1203
MvaI CCWGG 2 cut(s) 1633, 1766
MvnI CGCG 5 cut(s) 474, 695, 736, 849, 890
NaeI GCCGGC 1 cut(s) 1413
NarI GGCGCC 2 cut(s) 950, 1182
NciI CCSGG 4 cut(s) 389, 673, 926, 1736
NdeII GATC 6 cut(s) 63, 96, 1174, 1191, 1434, 1440
NgoMIV GCCGGC 1 cut(s) 1411
NmeAIII GCCGAG 3 cut(s) 237, 926, 1180
NspI RCATGY 3 cut(s) 1075, 1532, 1647
PciI ACATGT 1 cut(s) 1071
PciSI GCTCTTC 2 cut(s) 254, 1779
PcsI WCGNNNNNNNCGW 5 cut(s) 546, 558, 674, 819, 1152
PctI GAATGC 1 cut(s) 1203
PdiI GCCGGC 1 cut(s) 1413
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 4 cut(s) 352, 392, 934, 1160
PfoI TCCNGGA 1 cut(s) 387
PinAI ACCGGT 1 cut(s) 1012
PleI GAGTC 3 cut(s) 536, 850, 1102
PluTI GGCGCC 2 cut(s) 953, 1185
PpsI GAGTC 3 cut(s) 536, 850, 1102
Ppu21I YACGTR 2 cut(s) 55, 1479
PscI ACATGT 1 cut(s) 1071
Psp124BI GAGCTC 3 cut(s) 716, 1068, 1088
Psp6I CCWGG 2 cut(s) 1631, 1764
PspGI CCWGG 2 cut(s) 1631, 1764
PstI CTGCAG 1 cut(s) 1378
PsuI RGATCY 1 cut(s) 96
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 9 cut(s) 53, 374, 443, 470, 767, 912, 923, 1268, 1814
RsaNI GTAC 9 cut(s) 52, 373, 442, 469, 766, 911, 922, 1267, 1813
RseI CAYNNNNRTG 1 cut(s) 1053
Rsr2I CGGWCCG 2 cut(s) 851, 1170
RsrII CGGWCCG 2 cut(s) 851, 1170
SacI GAGCTC 3 cut(s) 716, 1068, 1088
SacII CCGCGG 2 cut(s) 475, 850
SapI GCTCTTC 2 cut(s) 254, 1779
SaqAI TTAA 5 cut(s) 362, 488, 534, 750, 1350
Sau3AI GATC 6 cut(s) 63, 96, 1174, 1191, 1434, 1440
SchI GAGTC 3 cut(s) 536, 850, 1103
ScrFI CCNGG 6 cut(s) 389, 673, 926, 1633, 1736, 1766
SduI GDGCHC 7 cut(s) 119, 716, 1068, 1088, 1122, 1510, 1742
SfaNI GCATC 8 cut(s) 132, 441, 577, 650, 926, 1045, 1527, 1567
SfcI CTRYAG 1 cut(s) 1374
SfoI GGCGCC 2 cut(s) 951, 1183
Sfr303I CCGCGG 2 cut(s) 475, 850
SgrAI CRCCGGYG 1 cut(s) 1411
SgrBI CCGCGG 2 cut(s) 475, 850
SinI GGWCC 7 cut(s) 267, 424, 625, 815, 851, 1137, 1170
SmiMI CAYNNNNRTG 1 cut(s) 1053
SmlI CTYRAG 1 cut(s) 1779
SmoI CTYRAG 1 cut(s) 1779
Sse9I AATT 5 cut(s) 871, 883, 941, 975, 1560
SspDI GGCGCC 2 cut(s) 949, 1181
SspMI CTAG 1 cut(s) 293
SstI GAGCTC 3 cut(s) 716, 1068, 1088
StyD4I CCNGG 6 cut(s) 387, 671, 924, 1631, 1734, 1764
StyI CCWWGG 2 cut(s) 1185, 1806
TaaI ACNGT 5 cut(s) 901, 910, 1025, 1170, 1572
TaiI ACGT 5 cut(s) 30, 57, 552, 621, 1481
TaqI TCGA 7 cut(s) 465, 540, 561, 644, 876, 1053, 1484
TaqII GACCGA 1 cut(s) 1154
TasI AATT 5 cut(s) 871, 883, 941, 975, 1560
TatI WGTACW 2 cut(s) 765, 1812
TauI GCSGC 7 cut(s) 49, 221, 801, 893, 997, 1411, 1715
TfiI GAWTC 4 cut(s) 352, 392, 934, 1160
Tru1I TTAA 5 cut(s) 362, 488, 534, 750, 1350
Tru9I TTAA 5 cut(s) 362, 488, 534, 750, 1350
TscAI CASTG 7 cut(s) 119, 434, 1030, 1120, 1378, 1696, 1846
TspDTI ATGAA 6 cut(s) 163, 202, 215, 773, 1606, 1811
TspGWI ACGGA 4 cut(s) 70, 621, 760, 1454
TspRI CASTG 7 cut(s) 119, 434, 1030, 1120, 1378, 1696, 1846
VpaK11BI GGWCC 7 cut(s) 267, 424, 625, 815, 851, 1137, 1170
XapI RAATTY 1 cut(s) 941
XceI RCATGY 3 cut(s) 1075, 1532, 1647
XcmI CCANNNNNNNNNTGG 2 cut(s) 1130, 1220
XmiI GTMKAC 1 cut(s) 1887
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 1 cut(s) 293
ZraI GACGTC 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.