RLG00000014076

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002177
Physical Location & Seq
Forward (+)
46692813 .. 46693190
378 bp
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UTR
Exon/CDS
Intron
RLM00000014076

Sequence Viewer

Length: 378 bp
ATGATCACGATTTTCTCGCTCTCCACGCTTCCCAATACTCTGGTCATGGGAATCCTGTTGCTGATCACCATGTACGACCACCGAATCCCGAAGTCCGACAACCTGATGGTCCAGGTCGTCGTGCTCCAGTGCATCATTTGGTACACTCTGTTACTCTTCCTCTTCGAGTACCGCAGCGCCAAGCTGCTGGTAATGGAGCAGTTCCCAGAGACCGCCGCCTCTATCGTCTCCTTCAAGGTCGACTCCGATGTCATGTCCCTCGATGGCCGTGACTTCCTCAAAACCGATGCCGAGATCGGCGACGACGGCAAGCTCCACGTCAAGGTGAGGAAGTCCAACGCGTCCAGACGGTCGCTCTCGGCAATGACGCTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000578 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009914 GO:0009921 GO:0009926 GO:0009942 GO:0009958 GO:0009966 GO:0009986 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0012506 GO:0015562 GO:0016020 GO:0016043 GO:0016049 GO:0016328 GO:0021700 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0030054 GO:0030154 GO:0031090 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048583 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0080161 GO:0090558 GO:0090627 GO:0098796 GO:0098797 GO:0099402 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

126

Amino Acids

14.18

Weight (kDa)

6.82

Isoelectric Point (pI)

29.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 2 - 111 2.5e-23 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AccI GTMKAC 1 cut(s) 240
AccII CGCG 1 cut(s) 341
AciI CCGC 3 cut(s) 172, 213, 216
AcoI YGGCCR 1 cut(s) 265
AfaI GTAC 3 cut(s) 74, 143, 170
AfiI CCNNNNNNNGG 1 cut(s) 322
AflIII ACRYGT 1 cut(s) 339
AgsI TTSAA 1 cut(s) 235
AjiI CACGTC 1 cut(s) 319
AjnI CCWGG 1 cut(s) 111
AluBI AGCT 2 cut(s) 184, 313
AluI AGCT 2 cut(s) 184, 313
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 2 cut(s) 203, 232
AoxI GGCC 1 cut(s) 265
ApeKI GCWGC 2 cut(s) 174, 184
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 58, 337
AvaII GGWCC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 171, 186
BccI CCATC 2 cut(s) 100, 257
BceAI ACGGC 2 cut(s) 252, 322
BciT130I CCWGG 1 cut(s) 113
BclI TGATCA 2 cut(s) 3, 63
BcoDI GTCTC 2 cut(s) 203, 232
BfaI CTAG 1 cut(s) 376
BfoI RGCGCY 1 cut(s) 180
BisI GCNGC 3 cut(s) 175, 185, 216
BlsI GCNGC 3 cut(s) 176, 186, 217
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 1 cut(s) 109
BmgBI CACGTC 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 109
BmrFI CCNGG 1 cut(s) 113
BmsI GCATC 2 cut(s) 141, 277
BpmI CTGGAG 1 cut(s) 110
BsaI GGTCTC 1 cut(s) 203
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse1I ACTGG 1 cut(s) 127
Bse3DI GCAATG 1 cut(s) 369
BseBI CCWGG 1 cut(s) 113
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMI GCAATG 1 cut(s) 369
BseNI ACTGG 1 cut(s) 127
BseXI GCAGC 2 cut(s) 171, 186
Bsh1236I CGCG 1 cut(s) 341
Bsh1285I CGRYCG 1 cut(s) 353
BshFI GGCC 1 cut(s) 267
BsiEI CGRYCG 1 cut(s) 353
BsiHKAI GWGCWC 1 cut(s) 126
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 2 cut(s) 203, 232
BsmBI CGTCTC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 241
BsnI GGCC 1 cut(s) 267
Bso31I GGTCTC 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 3 cut(s) 3, 63, 294
BspACI CCGC 3 cut(s) 172, 213, 216
BspANI GGCC 1 cut(s) 267
BspFNI CGCG 1 cut(s) 341
BspTNI GGTCTC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 1 cut(s) 127
BssMI GATC 3 cut(s) 3, 63, 294
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 351
Bst6I CTCTTC 2 cut(s) 161, 167
BstC8I GCNNGC 1 cut(s) 311
BstFNI CGCG 1 cut(s) 341
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 3 cut(s) 6, 66, 297
BstMAI GTCTC 2 cut(s) 203, 232
BstMBI GATC 3 cut(s) 3, 63, 294
BstMCI CGRYCG 1 cut(s) 353
BstMWI GCNNNNNNNGC 2 cut(s) 25, 306
BstNI CCWGG 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 111
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 2 cut(s) 171, 186
BstXI CCANNNNNNTGG 2 cut(s) 40, 187
BsuRI GGCC 1 cut(s) 267
BtrI CACGTC 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 134
Cac8I GCNNGC 1 cut(s) 311
CfoI GCGC 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 1 cut(s) 330
Csp6I GTAC 3 cut(s) 73, 142, 169
CviAII CATG 3 cut(s) 46, 70, 253
CviJI RGCY 3 cut(s) 184, 267, 313
CviKI_1 RGCY 3 cut(s) 184, 267, 313
CviQI GTAC 3 cut(s) 73, 142, 169
DpnI GATC 3 cut(s) 5, 65, 296
DpnII GATC 3 cut(s) 3, 63, 294
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 265
Eam1104I CTCTTC 2 cut(s) 161, 167
EarI CTCTTC 2 cut(s) 161, 167
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 111
Esp3I CGTCTC 1 cut(s) 232
FaeI CATG 3 cut(s) 49, 73, 256
FaiI YATR 3 cut(s) 47, 71, 254
FaqI GGGAC 1 cut(s) 241
FatI CATG 3 cut(s) 45, 69, 252
FbaI TGATCA 2 cut(s) 3, 63
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 3 cut(s) 175, 185, 216
Fsp4HI GCNGC 3 cut(s) 175, 185, 216
FspBI CTAG 1 cut(s) 376
GlaI GCGC 1 cut(s) 178
GluI GCNGC 3 cut(s) 175, 185, 216
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 1 cut(s) 267
HgaI GACGC 1 cut(s) 330
HhaI GCGC 1 cut(s) 179
Hin1II CATG 3 cut(s) 49, 73, 256
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 3 cut(s) 51, 84, 242
HphI GGTGA 2 cut(s) 58, 337
Hpy166II GTNNAC 2 cut(s) 144, 241
Hpy188I TCNGA 2 cut(s) 97, 247
Hpy188III TCNNGA 3 cut(s) 7, 88, 345
Hpy8I GTNNAC 2 cut(s) 144, 241
Hpy99I CGWCG 3 cut(s) 122, 305, 308
HpyAV CCTTC 1 cut(s) 241
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 1 cut(s) 132
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 306
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 3 cut(s) 49, 73, 256
HspAI GCGC 1 cut(s) 177
Ksp22I TGATCA 2 cut(s) 3, 63
Kzo9I GATC 3 cut(s) 3, 63, 294
LmnI GCTCC 3 cut(s) 129, 196, 318
LpnPI CCDG 9 cut(s) 26, 68, 98, 116, 125, 140, 173, 219, 358
Lsp1109I GCAGC 2 cut(s) 171, 186
LweI GCATC 2 cut(s) 141, 277
MaeI CTAG 1 cut(s) 376
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 2 cut(s) 150, 269
MalI GATC 3 cut(s) 5, 65, 296
MboI GATC 3 cut(s) 3, 63, 294
MboII GAAGA 2 cut(s) 148, 154
MhlI GDGCHC 1 cut(s) 126
MluI ACGCGT 1 cut(s) 339
MlyI GAGTC 1 cut(s) 236
MmeI TCCRAC 2 cut(s) 120, 360
MnlI CCTC 5 cut(s) 170, 229, 269, 287, 321
MspR9I CCNGG 1 cut(s) 113
MvaI CCWGG 1 cut(s) 113
MvnI CGCG 1 cut(s) 341
MwoI GCNNNNNNNGC 2 cut(s) 25, 306
NdeII GATC 3 cut(s) 3, 63, 294
NlaIII CATG 3 cut(s) 49, 73, 256
NmeAIII GCCGAG 2 cut(s) 316, 338
NmuCI GTSAC 1 cut(s) 269
PfeI GAWTC 2 cut(s) 51, 84
PkrI GCNGC 3 cut(s) 176, 186, 217
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 111
PspGI CCWGG 1 cut(s) 111
PspPI GGNCC 1 cut(s) 109
RsaI GTAC 3 cut(s) 74, 143, 170
RsaNI GTAC 3 cut(s) 73, 142, 169
SalI GTCGAC 1 cut(s) 239
SatI GCNGC 3 cut(s) 175, 185, 216
Sau3AI GATC 3 cut(s) 3, 63, 294
Sau96I GGNCC 1 cut(s) 109
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 1 cut(s) 113
SduI GDGCHC 1 cut(s) 126
SetI ASST 7 cut(s) 105, 117, 186, 240, 315, 321, 327
SfaNI GCATC 2 cut(s) 141, 277
SinI GGWCC 1 cut(s) 109
SsiI CCGC 3 cut(s) 172, 213, 216
SspMI CTAG 1 cut(s) 376
StyD4I CCNGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 351
TaiI ACGT 1 cut(s) 321
TaqI TCGA 3 cut(s) 165, 240, 261
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 2 cut(s) 51, 84
TscAI CASTG 1 cut(s) 134
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 2 cut(s) 174, 184
Tsp45I GTSAC 1 cut(s) 269
TspRI CASTG 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.