Rh4CG413400

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002177
Physical Location & Seq
Reverse (-)
65818232 .. 65822252
4021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG413400.1

Sequence Viewer

Length: 1977 bp
ATGATTTCCTGGAACGACCTCTACAAGGTCTTGACGGCGGTGGTGCCTCTCTACGTGGCCATGATCTTAGCCTACGGCTCCGTCCGGTGGTGGAAGATCTTCTCCCCGGACCAATGCTCCGGCATCAACCGCTTCGTCGCAATCTTCGCAGTTCCTCTTCTCTCCTTCAAGTTCATCTCCACCAACAACCCTTACACCATGAACTTCCGCTTCATCGCCGCCGACACTCTCCAGAAGATCATCATGCTCTTCGCCCTGGCCCTCTGGACCAACTTCACCCGAAACGGAAGCCTCGAGTGGATGATCACGATTTTCTCGCTCTCCACGCTTCCCAATACTCTGGTCATGGGAATCCCGCTGCTGATCGCCATGTACGGCCACCGAATCCCCGAGTCCGGCAGCCTGATGGTCCAGGTCGTCGTGCTCCAGTGCATCATTTGGTACACTCTGTTACTCTTCCTCTTCGAGTACCGCGGCGCCAAGCTGCTGATAATGGAGCAGTTCCCGGAGACCGCCGCCTCCATCGTCTCCTTCAAGGTCGACTCCGACGTCATGTCCCTCGACGGCCGCGACTTCCTCGAAACCGACGCCGAGATCGGCGACGACGGCAAGCTCCACGTCAAGGTGAGGAAGTCCAACGCGTCCAGACGGTCGCTCTCGGCAATGACTCCTCGGCCGTCCAACCTCACCGGAGCCGAGATTTACAGCCTCAGCTCTTCCCGGAATCCCACTCCCCGAGGCTCCAATTTCAACCCCTCCGATTTTTACTCCATGATGGGAGTCCAGGGCTTCAATACCCGCCACTCCAATTTCGGACCCGCCGACTTGTACTCGGTTCAGTCCTCCCGCGGTCCGACTCCCAGACCCTCCAATTTCGAAGAGAATTCTGCTCCAATATTGGCGCAGACGGTTACTTCTCCCCGGTTCGGGTTTTACCCGGCCCAGACTGTCCCCTCGTCGTACCCGGCTCCGAATCCGGAATTCTCCGCCCCCGTCACTAAAACAACCTCCAAAAATAACCAACCGCCGCCGCAGCAGCAGCAGCAGCAGGTTAACTCCAACAGTGGCAAAGCGAATCACGACGCGAAAGAGCTCCACATGTTCGTTTGGAGCTCCAGCAATTCCCCTGTTTCCGAAGGCGGAGGGCTCCACGTTTTCGGCGGACCTGACTTCGCCGCGTCGGAGCAATCGGGGCGGTCCGATCAGGGCGCCAAGGAGATCAGGATGTTGGTTGCTGACCACCCCCAGAATGGAGAAACCAAAGTGGCGGTTGCGGAGAGTGACGGCGATCACTACCACCACGGCGGAGAAGCCTTCACGTTCACCGGAAAGGCTGATCAGGGAGAGGAGGAGAGAGAAAAGGAGGGGCCAACCGGACTTAACAAGCAGGGGTCAAGCTCCACTGCCGAGCTACACGGCGGGAAAGGTGGAGGAGTGGGGGTTGATCACGAGTCAAGCGGCGGAAAACAAATGCCTCCGGCGAGCGTTATGACGCGTCTGATCTTGATCATGGTTTGGCGGAAGCTTATCCGCAATCCCAACACGTACTCTAGCCTCATCGGTCTCATCTGGTCGCTCATCGCTGTCCGGTGGGACGTTGCTATGCCAAAAATAATAGAGAAGTCGATCTCCATACTGTCCGATGCTGGGCTAGGAATGGCTATGTTCAGTTTAGGTCTTTTTATGGCACTTCAACCTAAGATAATAGCATGTGGAAACTCGGTGGCCGGGTTTGCAATGGCCGTGAGATTCCTCACTGGCCCGGCTGTGATGGCTGCAGCCTCCATTGCTGTTGGGCTACGTGGCACCCTCCTCCACTTAGCTATTGTCCAGGCTGCGCTTCCACAAGGAATTGTTCCCTTTGTCTTTGCCAAGGAGTACAATGTTCATCCATCCATTCTTAGCACTGCGGTTATATTCGGGATGTTGATAGCATTACCGATTACGCTGGTTTACTATATCGTTCTCGGACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000578 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009914 GO:0009921 GO:0009926 GO:0009942 GO:0009958 GO:0009966 GO:0009986 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0012506 GO:0015562 GO:0016020 GO:0016043 GO:0016049 GO:0016328 GO:0021700 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0030054 GO:0030154 GO:0031090 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048583 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0080161 GO:0090558 GO:0090627 GO:0098796 GO:0098797 GO:0099402 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

658

Amino Acids

71.58

Weight (kDa)

8.23

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 10 - 653 2.9e-127 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 548
AatII GACGTC 1 cut(s) 552
Acc36I ACCTGC 1 cut(s) 1039
AccB1I GGYRCC 4 cut(s) 43, 476, 1208, 1805
AccI GTMKAC 1 cut(s) 540
AccII CGCG 7 cut(s) 474, 570, 641, 849, 1085, 1178, 1495
AccIII TCCGGA 1 cut(s) 976
AcoI YGGCCR 6 cut(s) 57, 376, 565, 674, 1725, 1740
AcsI RAATTY 2 cut(s) 883, 980
AcyI GRCGYC 4 cut(s) 477, 549, 588, 1209
AfaI GTAC 7 cut(s) 374, 443, 470, 830, 962, 1547, 1880
AfiI CCNNNNNNNGG 8 cut(s) 25, 87, 395, 622, 926, 927, 1250, 1647
AflIII ACRYGT 4 cut(s) 639, 1098, 1493, 1542
AgsI TTSAA 5 cut(s) 169, 535, 751, 793, 1694
AhdI GACNNNNNGTC 1 cut(s) 553
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 5 cut(s) 8, 255, 411, 783, 1830
AluBI AGCT 9 cut(s) 484, 613, 714, 1093, 1113, 1398, 1411, 1525, 1823
AluI AGCT 9 cut(s) 484, 613, 714, 1093, 1113, 1398, 1411, 1525, 1823
Alw21I GWGCWC 3 cut(s) 426, 1095, 1115
Alw26I GTCTC 3 cut(s) 503, 532, 1568
Ama87I CYCGRG 3 cut(s) 293, 389, 735
Aor13HI TCCGGA 1 cut(s) 976
ApoI RAATTY 2 cut(s) 883, 980
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 4 cut(s) 479, 904, 1211, 1840
AsuC2I CCSGG 8 cut(s) 107, 506, 721, 922, 938, 965, 1729, 1763
AsuHPI GGTGA 4 cut(s) 268, 637, 679, 1315
AsuII TTCGAA 1 cut(s) 876
AvaI CYCGRG 3 cut(s) 293, 389, 735
AvaII GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1163, 1197
BalI TGGCCA 1 cut(s) 59
BanI GGYRCC 4 cut(s) 43, 476, 1208, 1805
BanII GRGCYC 3 cut(s) 1095, 1115, 1149
BauI CACGAG 1 cut(s) 1448
Bbv12I GWGCWC 3 cut(s) 426, 1095, 1115
BbvCI CCTCAGC 1 cut(s) 710
BccI CCATC 5 cut(s) 400, 530, 769, 1765, 1900
BciT130I CCWGG 5 cut(s) 10, 257, 413, 785, 1832
BclI TGATCA 4 cut(s) 303, 1336, 1444, 1506
BcnI CCSGG 8 cut(s) 107, 506, 721, 922, 938, 965, 1729, 1763
BcoDI GTCTC 3 cut(s) 503, 532, 1568
BfaI CTAG 2 cut(s) 1551, 1652
BfmI CTRYAG 1 cut(s) 1776
BfoI RGCGCY 2 cut(s) 480, 1212
BfuAI ACCTGC 1 cut(s) 1039
BglII AGATCT 1 cut(s) 96
Bme18I GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1163, 1197
BmeRI GACNNNNNGTC 1 cut(s) 553
BmeT110I CYCGRG 3 cut(s) 293, 389, 735
BmgBI CACGTC 1 cut(s) 619
BmsI GCATC 3 cut(s) 132, 441, 1633
BpmI CTGGAG 3 cut(s) 215, 410, 1099
Bpu10I CCTNAGC 1 cut(s) 710
Bpu14I TTCGAA 1 cut(s) 876
BpuMI CCSGG 8 cut(s) 107, 506, 721, 922, 938, 965, 1729, 1763
BsaAI YACGTR 3 cut(s) 55, 1545, 1802
BsaBI GATNNNNATC 1 cut(s) 1505
BsaHI GRCGYC 4 cut(s) 477, 549, 588, 1209
BsaI GGTCTC 2 cut(s) 503, 1568
BsaWI WCCGGW 6 cut(s) 84, 689, 976, 1325, 1373, 1587
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 1425, 1455
Bsc4I CCNNNNNNNGG 8 cut(s) 25, 87, 395, 622, 926, 927, 1250, 1647
Bse1I ACTGG 2 cut(s) 427, 1762
Bse3DI GCAATG 3 cut(s) 669, 1743, 1785
Bse8I GATNNNNATC 1 cut(s) 1505
BseAI TCCGGA 1 cut(s) 976
BseBI CCWGG 5 cut(s) 10, 257, 413, 785, 1832
BseGI GGATG 5 cut(s) 306, 1230, 1888, 1892, 1929
BseJI GATNNNNATC 1 cut(s) 1505
BseLI CCNNNNNNNGG 8 cut(s) 25, 87, 395, 622, 926, 927, 1250, 1647
BseMI GCAATG 3 cut(s) 669, 1743, 1785
BseMII CTCAG 1 cut(s) 724
BseNI ACTGG 2 cut(s) 427, 1762
BseRI GAGGAG 5 cut(s) 660, 1361, 1364, 1446, 1802
BseX3I CGGCCG 2 cut(s) 565, 674
BseYI CCCAGC 1 cut(s) 1646
Bsh1236I CGCG 7 cut(s) 474, 570, 641, 849, 1085, 1178, 1495
Bsh1285I CGRYCG 3 cut(s) 568, 653, 677
BshNI GGYRCC 4 cut(s) 43, 476, 1208, 1805
BsiEI CGRYCG 3 cut(s) 568, 653, 677
BsiHKAI GWGCWC 3 cut(s) 426, 1095, 1115
BsiHKCI CYCGRG 3 cut(s) 293, 389, 735
BslFI GGGAC 3 cut(s) 541, 935, 1607
BslI CCNNNNNNNGG 8 cut(s) 25, 87, 395, 622, 926, 927, 1250, 1647
BsmAI GTCTC 3 cut(s) 503, 532, 1568
BsmBI CGTCTC 1 cut(s) 532
BsmFI GGGAC 3 cut(s) 541, 935, 1607
Bso31I GGTCTC 2 cut(s) 503, 1568
BsoBI CYCGRG 3 cut(s) 293, 389, 735
Bsp119I TTCGAA 1 cut(s) 876
Bsp1286I GDGCHC 4 cut(s) 426, 1095, 1115, 1149
Bsp13I TCCGGA 1 cut(s) 976
BspCNI CTCAG 1 cut(s) 723
BspEI TCCGGA 1 cut(s) 976
BspFNI CGCG 7 cut(s) 474, 570, 641, 849, 1085, 1178, 1495
BspMAI CTGCAG 1 cut(s) 1780
BspMI ACCTGC 1 cut(s) 1039
BspQI GCTCTTC 2 cut(s) 254, 721
BspT104I TTCGAA 1 cut(s) 876
BspT107I GGYRCC 4 cut(s) 43, 476, 1208, 1805
BspTNI GGTCTC 2 cut(s) 503, 1568
BsrDI GCAATG 3 cut(s) 669, 1743, 1785
BsrI ACTGG 2 cut(s) 427, 1762
BssNI GRCGYC 4 cut(s) 477, 549, 588, 1209
BssSI CACGAG 1 cut(s) 1448
BssT1I CCWWGG 2 cut(s) 1212, 1872
Bst2BI CACGAG 1 cut(s) 1448
Bst2UI CCWGG 5 cut(s) 10, 257, 413, 785, 1832
Bst4CI ACNGT 6 cut(s) 651, 910, 949, 1064, 1638, 1974
Bst6I CTCTTC 6 cut(s) 162, 254, 461, 467, 721, 873
BstACI GRCGYC 4 cut(s) 477, 549, 588, 1209
BstBAI YACGTR 3 cut(s) 55, 1545, 1802
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 2 cut(s) 611, 1483
BstDEI CTNAG 5 cut(s) 67, 710, 1698, 1819, 1901
BstDSI CCRYGG 3 cut(s) 472, 847, 1300
BstENI CCTNNNNNAGG 1 cut(s) 23
BstF5I GGATG 5 cut(s) 306, 1230, 1888, 1892, 1929
BstFNI CGCG 7 cut(s) 474, 570, 641, 849, 1085, 1178, 1495
BstH2I RGCGCY 2 cut(s) 480, 1212
BstHHI GCGC 4 cut(s) 479, 904, 1211, 1840
BstMAI GTCTC 3 cut(s) 503, 532, 1568
BstMCI CGRYCG 3 cut(s) 568, 653, 677
BstNI CCWGG 5 cut(s) 10, 257, 413, 785, 1832
BstNSI RCATGY 2 cut(s) 1102, 1713
BstSFI CTRYAG 1 cut(s) 1776
BstUI CGCG 7 cut(s) 474, 570, 641, 849, 1085, 1178, 1495
BstX2I RGATCY 1 cut(s) 96
BstXI CCANNNNNNTGG 1 cut(s) 340
BstYI RGATCY 1 cut(s) 96
BstZI CGGCCG 2 cut(s) 565, 674
BtgI CCRYGG 3 cut(s) 472, 847, 1300
BtgZI GCGATG 2 cut(s) 199, 1564
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 5 cut(s) 306, 1230, 1888, 1892, 1929
BtsI GCAGTG 2 cut(s) 1401, 1905
BtsIMutI CAGTG 5 cut(s) 434, 1069, 1401, 1755, 1905
BveI ACCTGC 1 cut(s) 1039
Cac8I GCNNGC 2 cut(s) 611, 1483
CfoI GCGC 4 cut(s) 479, 904, 1211, 1840
Cfr42I CCGCGG 2 cut(s) 475, 850
CpoI CGGWCCG 2 cut(s) 851, 1197
CseI GACGC 6 cut(s) 596, 630, 1091, 1167, 1484, 1501
Csp6I GTAC 7 cut(s) 373, 442, 469, 829, 961, 1546, 1879
CspI CGGWCCG 2 cut(s) 851, 1197
CviQI GTAC 7 cut(s) 373, 442, 469, 829, 961, 1546, 1879
DdeI CTNAG 5 cut(s) 67, 710, 1698, 1819, 1901
DinI GGCGCC 2 cut(s) 478, 1210
DrdI GACNNNNNNGTC 1 cut(s) 548
DriI GACNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 1 cut(s) 548
EaeI YGGCCR 6 cut(s) 57, 376, 565, 674, 1725, 1740
EagI CGGCCG 2 cut(s) 565, 674
Eam1104I CTCTTC 6 cut(s) 162, 254, 461, 467, 721, 873
Eam1105I GACNNNNNGTC 1 cut(s) 553
EarI CTCTTC 6 cut(s) 162, 254, 461, 467, 721, 873
EciI GGCGGA 6 cut(s) 976, 1155, 1176, 1320, 1476, 1534
Ecl136II GAGCTC 2 cut(s) 1093, 1113
EclXI CGGCCG 2 cut(s) 565, 674
Eco130I CCWWGG 2 cut(s) 1212, 1872
Eco24I GRGCYC 3 cut(s) 1095, 1115, 1149
Eco31I GGTCTC 2 cut(s) 503, 1568
Eco47I GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1163, 1197
Eco52I CGGCCG 2 cut(s) 565, 674
Eco53kI GAGCTC 2 cut(s) 1093, 1113
Eco88I CYCGRG 3 cut(s) 293, 389, 735
EcoICRI GAGCTC 2 cut(s) 1093, 1113
EcoNI CCTNNNNNAGG 1 cut(s) 23
EcoRI GAATTC 2 cut(s) 883, 980
EcoRII CCWGG 5 cut(s) 8, 255, 411, 783, 1830
EcoT14I CCWWGG 2 cut(s) 1212, 1872
EcoT38I GRGCYC 3 cut(s) 1095, 1115, 1149
EgeI GGCGCC 2 cut(s) 478, 1210
EheI GGCGCC 2 cut(s) 478, 1210
ErhI CCWWGG 2 cut(s) 1212, 1872
Esp3I CGTCTC 1 cut(s) 532
FaqI GGGAC 3 cut(s) 541, 935, 1607
FauI CCCGC 5 cut(s) 363, 806, 826, 854, 1412
FbaI TGATCA 4 cut(s) 303, 1336, 1444, 1506
FblI GTMKAC 1 cut(s) 540
FokI GGATG 5 cut(s) 313, 1237, 1875, 1879, 1936
FriOI GRGCYC 3 cut(s) 1095, 1115, 1149
FspBI CTAG 2 cut(s) 1551, 1652
GlaI GCGC 4 cut(s) 478, 903, 1210, 1839
GsaI CCCAGC 1 cut(s) 1650
GsuI CTGGAG 3 cut(s) 215, 410, 1099
HaeII RGCGCY 2 cut(s) 480, 1212
HgaI GACGC 6 cut(s) 596, 630, 1091, 1167, 1484, 1501
HhaI GCGC 4 cut(s) 479, 904, 1211, 1840
Hin1I GRCGYC 4 cut(s) 477, 549, 588, 1209
Hin6I GCGC 4 cut(s) 477, 902, 1209, 1838
HinP1I GCGC 4 cut(s) 477, 902, 1209, 1838
HincII GTYRAC 2 cut(s) 541, 1054
HindII GTYRAC 2 cut(s) 541, 1054
HindIII AAGCTT 1 cut(s) 1523
HpaI GTTAAC 1 cut(s) 1054
HphI GGTGA 4 cut(s) 268, 637, 679, 1315
Hpy166II GTNNAC 5 cut(s) 444, 541, 1054, 1323, 1954
Hpy8I GTNNAC 5 cut(s) 444, 541, 1054, 1323, 1954
HpyAV CCTTC 4 cut(s) 175, 541, 1130, 1324
HpyCH4III ACNGT 6 cut(s) 651, 910, 949, 1064, 1638, 1974
HpyCH4IV ACGT 8 cut(s) 54, 549, 618, 1152, 1319, 1544, 1596, 1801
HpyCH4V TGCA 3 cut(s) 432, 1736, 1778
HpyF3I CTNAG 5 cut(s) 67, 710, 1698, 1819, 1901
HpySE526I ACGT 8 cut(s) 54, 549, 618, 1152, 1319, 1544, 1596, 1801
Hsp92I GRCGYC 4 cut(s) 477, 549, 588, 1209
HspAI GCGC 4 cut(s) 477, 902, 1209, 1838
KasI GGCGCC 2 cut(s) 476, 1208
Kpn2I TCCGGA 1 cut(s) 976
Ksp22I TGATCA 4 cut(s) 303, 1336, 1444, 1506
KspAI GTTAAC 1 cut(s) 1054
KspI CCGCGG 2 cut(s) 475, 850
LguI GCTCTTC 2 cut(s) 254, 721
LweI GCATC 3 cut(s) 132, 441, 1633
MaeI CTAG 2 cut(s) 1551, 1652
MaeII ACGT 8 cut(s) 54, 549, 618, 1152, 1319, 1544, 1596, 1801
MaeIII GTNAC 4 cut(s) 450, 910, 994, 1280
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 4 cut(s) 426, 1095, 1115, 1149
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 7 cut(s) 745, 808, 871, 883, 980, 1120, 1851
MluI ACGCGT 2 cut(s) 639, 1493
MluNI TGGCCA 1 cut(s) 59
Mly113I GGCGCC 2 cut(s) 477, 1209
MlyI GAGTC 6 cut(s) 401, 536, 661, 789, 850, 1460
MmeI TCCRAC 6 cut(s) 570, 660, 705, 878, 1083, 1161
Mox20I TGGCCA 1 cut(s) 59
MroI TCCGGA 1 cut(s) 976
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 1 cut(s) 59
MseI TTAA 2 cut(s) 1053, 1380
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 3 cut(s) 358, 474, 849
MvaI CCWGG 5 cut(s) 10, 257, 413, 785, 1832
MvnI CGCG 7 cut(s) 474, 570, 641, 849, 1085, 1178, 1495
NarI GGCGCC 2 cut(s) 477, 1209
NciI CCSGG 8 cut(s) 107, 506, 721, 922, 938, 965, 1729, 1763
NmeAIII GCCGAG 5 cut(s) 616, 638, 652, 721, 1432
NmuCI GTSAC 2 cut(s) 994, 1280
NspI RCATGY 2 cut(s) 1102, 1713
NspV TTCGAA 1 cut(s) 876
PaeR7I CTCGAG 1 cut(s) 293
PciI ACATGT 1 cut(s) 1098
PciSI GCTCTTC 2 cut(s) 254, 721
PcsI WCGNNNNNNNCGW 4 cut(s) 291, 546, 567, 819
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 6 cut(s) 351, 384, 724, 973, 1075, 1749
PfoI TCCNGGA 3 cut(s) 8, 504, 719
PleI GAGTC 6 cut(s) 400, 536, 661, 788, 850, 1459
PluTI GGCGCC 2 cut(s) 480, 1212
PpsI GAGTC 6 cut(s) 400, 536, 661, 788, 850, 1459
Ppu21I YACGTR 3 cut(s) 55, 1545, 1802
PscI ACATGT 1 cut(s) 1098
Psp124BI GAGCTC 2 cut(s) 1095, 1115
Psp6I CCWGG 5 cut(s) 8, 255, 411, 783, 1830
PspFI CCCAGC 1 cut(s) 1646
PspGI CCWGG 5 cut(s) 8, 255, 411, 783, 1830
PspXI VCTCGAGB 1 cut(s) 293
PsrI GAACNNNNNNTAC 1 cut(s) 37
PstI CTGCAG 1 cut(s) 1780
PsuI RGATCY 1 cut(s) 96
RsaI GTAC 7 cut(s) 374, 443, 470, 830, 962, 1547, 1880
RsaNI GTAC 7 cut(s) 373, 442, 469, 829, 961, 1546, 1879
Rsr2I CGGWCCG 2 cut(s) 851, 1197
RsrII CGGWCCG 2 cut(s) 851, 1197
SacI GAGCTC 2 cut(s) 1095, 1115
SacII CCGCGG 2 cut(s) 475, 850
SalI GTCGAC 1 cut(s) 539
SapI GCTCTTC 2 cut(s) 254, 721
SaqAI TTAA 2 cut(s) 1053, 1380
SchI GAGTC 6 cut(s) 401, 536, 661, 789, 850, 1460
SduI GDGCHC 4 cut(s) 426, 1095, 1115, 1149
SfaNI GCATC 3 cut(s) 132, 441, 1633
SfcI CTRYAG 1 cut(s) 1776
SfoI GGCGCC 2 cut(s) 478, 1210
Sfr274I CTCGAG 1 cut(s) 293
Sfr303I CCGCGG 2 cut(s) 475, 850
SfuI TTCGAA 1 cut(s) 876
SgrBI CCGCGG 2 cut(s) 475, 850
SinI GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1163, 1197
SlaI CTCGAG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 7 cut(s) 745, 808, 871, 883, 980, 1120, 1851
SspDI GGCGCC 2 cut(s) 476, 1208
SspI AATATT 1 cut(s) 897
SspMI CTAG 2 cut(s) 1551, 1652
SstI GAGCTC 2 cut(s) 1095, 1115
StyI CCWWGG 2 cut(s) 1212, 1872
TaaI ACNGT 6 cut(s) 651, 910, 949, 1064, 1638, 1974
TaiI ACGT 8 cut(s) 57, 552, 621, 1155, 1322, 1547, 1599, 1804
TaqI TCGA 7 cut(s) 294, 465, 540, 561, 579, 876, 1625
TaqII GACCGA 1 cut(s) 1550
TasI AATT 7 cut(s) 745, 808, 871, 883, 980, 1120, 1851
TatI WGTACW 2 cut(s) 828, 1878
TauI GCSGC 8 cut(s) 221, 477, 518, 570, 1030, 1033, 1178, 1461
TfiI GAWTC 6 cut(s) 351, 384, 724, 973, 1075, 1749
Tru1I TTAA 2 cut(s) 1053, 1380
Tru9I TTAA 2 cut(s) 1053, 1380
TscAI CASTG 5 cut(s) 434, 1069, 1408, 1762, 1912
TseFI GTSAC 2 cut(s) 994, 1280
Tsp45I GTSAC 2 cut(s) 994, 1280
TspDTI ATGAA 4 cut(s) 163, 202, 215, 1877
TspGWI ACGGA 2 cut(s) 70, 300
TspRI CASTG 5 cut(s) 434, 1069, 1408, 1762, 1912
VpaK11BI GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1163, 1197
XagI CCTNNNNNAGG 1 cut(s) 23
XapI RAATTY 2 cut(s) 883, 980
XceI RCATGY 2 cut(s) 1102, 1713
XcmI CCANNNNNNNNNTGG 1 cut(s) 1247
XhoI CTCGAG 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 1551, 1652
ZraI GACGTC 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.