Rw4G033160

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002177
Physical Location & Seq
Reverse (-)
60257436 .. 60261615
4180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033160.1

Sequence Viewer

Length: 1878 bp
ATGCTCCGGCATCAACCGTTTCGTCGCAATCTTCGCAGTTCCTCTTCTCTCCTTCCACTTCATCTCCACCAACGACCCTTACACCATGAACTTCCGCTTCATCGCCGCCGACACTCTCCAGAAGATCATCATGCTCTTCGCCCTGGCCCTTGGACCAACTTCACCCGAAACGGAAGCCTCGAGTGGATGATCACGATTTTCTCGCTCTCCACGCTTCCCAATACTCTGGTCATGGGAATCCCGCTGCTGATCGCCATGTACGGCCACCGAATCCCGGAGTCCGGCAGCCTCATGGTCCAGGTCGTCGTGCTCCAGTGCATCATCTGGTACACTCTGTTACTCTTCCTCTTCGAGTACCGCGGCGCCAAGCTGCTGATAATGGAGCAGTTCCCGGAGACCGCCGCCTCCATCGTCTCCTTCAAGGTCGACTCCGACGTCATGTCCCTCGACGGCCGCGACTTCCTCGAAACCGACGCCGAGATCGGCGACGACGGCAAGCTCCACGTCAAGGTGAGGAAGTCCAACGCGTCCAGAAGGTCCCTCTCCGCAATGACGCCTCGGCCGTCCAACCTCACCGGAGCCGAGATTTACAGCCTCAGCTCCTCCCGGAATCCCACTCCCCGTGGCTCCAATTTCAACCCCTCCGATTTTTACTCCATGATGGGAGTCCAGGGCTTCAATACCCGCCACTCCAATTTCGGACCCGCCGACTTGTACTCGGTTCAGTCCTCCCGAGGTCCGACTCCCAGACCCTCCAATTTCGAAGAGAATTCTGCTCCAATGGCGCAGACCGTTACTTCTCCCCGGTTCGGGTTTTACCCGGCCCAGACTGTCCCCTCCTCGTACCCGGCTCCGAATCCGGAATTCTCCGCCCCCCTCACTAAAACGACCTCCAAAAATAACCAACCGCAACCCCAACCCCAACCGCAACCGCCGCCGCAGCAGGTTAACTCCAACAACAGTAGCAAAGCGAATCACGACGCGAAAGAGCTCCACATGTTCGTTTGGAGCTCCAGCAATTCCCCTGTTTCCGAAGGCGGAGGGCTCCACGTCTTCGGCGGACCTGACTTCGCCGCGTCGGAGCAATCGGGGCGGTCCGATCAGGGCGCCAAGGAGATCAGGATGTTGGTTGCTGACCACCCCCAGAATGGAGAAACCAAAGTGGCGGTTGCGGAGAGTGACGGCGATCACTACCACCACGGCGGAGAAGCCTTCACGTTCACCGGAAAGGTTGATCAGGGAGAGGAGGAGAGAGAAAAGGAGGGGCCAACGGGACTTAACAAGCAGGGGTCAAGCTCGACTGCCGAGCTACACGGGAAAGGAGGAGCAGGAGTACTGGGGGTTGATCACGAGTCAAGCGGCGGAAAACAAATGCCTCCGGCGAGCGTTATGACGCGTCTGATCTTGATCATGGTTTGGCGGAAGCTTATCCGCAATCCCAACACGTACTCTAGCCTCATCGGTCTCATCTGGTCGCTCATCGCTTTCCGGTGGGGCGTTGCTATGCCAAAAATAATAGAGAAGTCGATCTCCATTCTGTCCGATGCTGGGCTAGGAATGGCTATGTTCAGTTTAGGTCTTTTTATGGCACTTCAACCTAAGATAATAGCATGTGGAAACTCGGTGGCCGGGTTTGCAATGGCCGTGAGATTCCTCACTGGCCCGGCTGTGATGGCTGCAGCCTCCATTGCTGTTGGGCTACGTGGCACCCTCCTCCACTTAGCTATTGTCCAGGCTGCACTTCCTCAAGGAATTGTTCCCTTTGTCTTTGCCAAGGAGTACAATGTTCATCCATCCATTCTTAGCACTGCGGTTATATTCGGGATGTTGATAGCATTACCGATTACGCTGGTTTACTATATCGTTCTCGGACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000578 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009914 GO:0009921 GO:0009926 GO:0009942 GO:0009958 GO:0009966 GO:0009986 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0012506 GO:0015562 GO:0016020 GO:0016043 GO:0016049 GO:0016328 GO:0021700 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0030054 GO:0030154 GO:0031090 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048583 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0080161 GO:0090558 GO:0090627 GO:0098796 GO:0098797 GO:0099402 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

625

Amino Acids

68.13

Weight (kDa)

8.99

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 51 - 620 5.2e-94 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 434
AatII GACGTC 1 cut(s) 438
Acc36I ACCTGC 1 cut(s) 934
AccB1I GGYRCC 3 cut(s) 362, 1106, 1706
AccI GTMKAC 1 cut(s) 426
AccII CGCG 6 cut(s) 360, 456, 527, 983, 1076, 1396
AccIII TCCGGA 1 cut(s) 859
AcoI YGGCCR 5 cut(s) 262, 451, 560, 1626, 1641
AcsI RAATTY 2 cut(s) 769, 863
AcyI GRCGYC 5 cut(s) 363, 435, 474, 554, 1107
AfaI GTAC 8 cut(s) 260, 329, 356, 716, 845, 1335, 1448, 1781
AfiI CCNNNNNNNGG 7 cut(s) 274, 281, 508, 809, 810, 1148, 1548
AflIII ACRYGT 4 cut(s) 525, 996, 1394, 1443
AgsI TTSAA 4 cut(s) 421, 637, 679, 1595
AhdI GACNNNNNGTC 1 cut(s) 439
AjiI CACGTC 2 cut(s) 505, 1051
AjnI CCWGG 4 cut(s) 142, 297, 669, 1731
AluBI AGCT 9 cut(s) 370, 499, 600, 991, 1011, 1296, 1309, 1426, 1724
AluI AGCT 9 cut(s) 370, 499, 600, 991, 1011, 1296, 1309, 1426, 1724
Alw21I GWGCWC 3 cut(s) 312, 993, 1013
Alw26I GTCTC 3 cut(s) 389, 418, 1469
Ama87I CYCGRG 2 cut(s) 179, 732
Aor13HI TCCGGA 1 cut(s) 859
AoxI GGCC 9 cut(s) 145, 262, 451, 560, 822, 1265, 1626, 1641, 1660
ApeKI GCWGC 7 cut(s) 244, 285, 370, 940, 1676, 1679, 1736
ApoI RAATTY 2 cut(s) 769, 863
AspLEI GCGC 3 cut(s) 365, 787, 1109
AsuC2I CCSGG 8 cut(s) 275, 392, 607, 805, 821, 848, 1630, 1664
AsuHPI GGTGA 4 cut(s) 154, 523, 565, 1213
AsuII TTCGAA 1 cut(s) 762
AvaI CYCGRG 2 cut(s) 179, 732
AvaII GGWCC 7 cut(s) 153, 295, 537, 701, 737, 1061, 1095
BanI GGYRCC 3 cut(s) 362, 1106, 1706
BanII GRGCYC 3 cut(s) 993, 1013, 1047
BauI CACGAG 1 cut(s) 1349
BbsI GAAGAC 1 cut(s) 1045
Bbv12I GWGCWC 3 cut(s) 312, 993, 1013
BbvCI CCTCAGC 1 cut(s) 596
BbvI GCAGC 7 cut(s) 231, 297, 357, 952, 1663, 1691, 1723
BccI CCATC 4 cut(s) 416, 655, 1666, 1801
BceAI ACGGC 7 cut(s) 277, 466, 508, 547, 1198, 1216, 1628
BciT130I CCWGG 4 cut(s) 144, 299, 671, 1733
BclI TGATCA 4 cut(s) 189, 1234, 1345, 1407
BcnI CCSGG 8 cut(s) 275, 392, 607, 805, 821, 848, 1630, 1664
BcoDI GTCTC 3 cut(s) 389, 418, 1469
BfaI CTAG 2 cut(s) 1452, 1553
BfmI CTRYAG 1 cut(s) 1677
BfoI RGCGCY 2 cut(s) 366, 1110
BfuAI ACCTGC 1 cut(s) 934
BmcAI AGTACT 1 cut(s) 1335
Bme18I GGWCC 7 cut(s) 153, 295, 537, 701, 737, 1061, 1095
BmeRI GACNNNNNGTC 1 cut(s) 439
BmeT110I CYCGRG 2 cut(s) 179, 732
BmgBI CACGTC 2 cut(s) 505, 1051
BmrI ACTGGG 1 cut(s) 1346
BmsI GCATC 3 cut(s) 19, 327, 1534
BmuI ACTGGG 1 cut(s) 1346
BpiI GAAGAC 1 cut(s) 1045
BpmI CTGGAG 3 cut(s) 102, 296, 997
Bpu10I CCTNAGC 1 cut(s) 596
Bpu14I TTCGAA 1 cut(s) 762
BpuEI CTTGAG 1 cut(s) 1731
BpuMI CCSGG 8 cut(s) 275, 392, 607, 805, 821, 848, 1630, 1664
BsaAI YACGTR 2 cut(s) 1446, 1703
BsaBI GATNNNNATC 1 cut(s) 1406
BsaHI GRCGYC 5 cut(s) 363, 435, 474, 554, 1107
BsaI GGTCTC 2 cut(s) 389, 1469
BsaWI WCCGGW 4 cut(s) 575, 859, 1223, 1488
BsaXI ACNNNNNCTCC 4 cut(s) 48, 78, 1317, 1347
Bsc4I CCNNNNNNNGG 7 cut(s) 274, 281, 508, 809, 810, 1148, 1548
Bse1I ACTGG 3 cut(s) 313, 1341, 1663
Bse3DI GCAATG 3 cut(s) 555, 1644, 1686
Bse8I GATNNNNATC 1 cut(s) 1406
BseAI TCCGGA 1 cut(s) 859
BseBI CCWGG 4 cut(s) 144, 299, 671, 1733
BseGI GGATG 5 cut(s) 192, 1128, 1789, 1793, 1830
BseJI GATNNNNATC 1 cut(s) 1406
BseLI CCNNNNNNNGG 7 cut(s) 274, 281, 508, 809, 810, 1148, 1548
BseMI GCAATG 3 cut(s) 555, 1644, 1686
BseMII CTCAG 1 cut(s) 610
BseNI ACTGG 3 cut(s) 313, 1341, 1663
BseRI GAGGAG 6 cut(s) 592, 829, 1259, 1262, 1338, 1703
BseX3I CGGCCG 2 cut(s) 451, 560
BseXI GCAGC 7 cut(s) 231, 297, 357, 952, 1663, 1691, 1723
BseYI CCCAGC 1 cut(s) 1547
BsgI GTGCAG 1 cut(s) 1722
Bsh1236I CGCG 6 cut(s) 360, 456, 527, 983, 1076, 1396
Bsh1285I CGRYCG 2 cut(s) 454, 563
BshFI GGCC 9 cut(s) 147, 264, 453, 562, 824, 1267, 1628, 1643, 1662
BshNI GGYRCC 3 cut(s) 362, 1106, 1706
BsiEI CGRYCG 2 cut(s) 454, 563
BsiHKAI GWGCWC 3 cut(s) 312, 993, 1013
BsiHKCI CYCGRG 2 cut(s) 179, 732
BslFI GGGAC 4 cut(s) 427, 523, 818, 1287
BslI CCNNNNNNNGG 7 cut(s) 274, 281, 508, 809, 810, 1148, 1548
BsmAI GTCTC 3 cut(s) 389, 418, 1469
BsmBI CGTCTC 1 cut(s) 418
BsmFI GGGAC 4 cut(s) 427, 523, 818, 1287
BsnI GGCC 9 cut(s) 147, 264, 453, 562, 824, 1267, 1628, 1643, 1662
Bso31I GGTCTC 2 cut(s) 389, 1469
BsoBI CYCGRG 2 cut(s) 179, 732
Bsp119I TTCGAA 1 cut(s) 762
Bsp1286I GDGCHC 4 cut(s) 312, 993, 1013, 1047
Bsp13I TCCGGA 1 cut(s) 859
BspANI GGCC 9 cut(s) 147, 264, 453, 562, 824, 1267, 1628, 1643, 1662
BspCNI CTCAG 1 cut(s) 609
BspEI TCCGGA 1 cut(s) 859
BspFNI CGCG 6 cut(s) 360, 456, 527, 983, 1076, 1396
BspMAI CTGCAG 1 cut(s) 1681
BspMI ACCTGC 1 cut(s) 934
BspQI GCTCTTC 1 cut(s) 141
BspT104I TTCGAA 1 cut(s) 762
BspT107I GGYRCC 3 cut(s) 362, 1106, 1706
BspTNI GGTCTC 2 cut(s) 389, 1469
BsrDI GCAATG 3 cut(s) 555, 1644, 1686
BsrI ACTGG 3 cut(s) 313, 1341, 1663
BssNI GRCGYC 5 cut(s) 363, 435, 474, 554, 1107
BssSI CACGAG 1 cut(s) 1349
BssT1I CCWWGG 3 cut(s) 149, 1110, 1773
Bst2BI CACGAG 1 cut(s) 1349
Bst2UI CCWGG 4 cut(s) 144, 299, 671, 1733
Bst4CI ACNGT 5 cut(s) 18, 793, 832, 962, 1875
Bst6I CTCTTC 5 cut(s) 49, 141, 347, 353, 759
BstACI GRCGYC 5 cut(s) 363, 435, 474, 554, 1107
BstBAI YACGTR 2 cut(s) 1446, 1703
BstBI TTCGAA 1 cut(s) 762
BstC8I GCNNGC 2 cut(s) 497, 1384
BstDEI CTNAG 4 cut(s) 596, 1599, 1720, 1802
BstDSI CCRYGG 3 cut(s) 358, 622, 1198
BstF5I GGATG 5 cut(s) 192, 1128, 1789, 1793, 1830
BstFNI CGCG 6 cut(s) 360, 456, 527, 983, 1076, 1396
BstH2I RGCGCY 2 cut(s) 366, 1110
BstHHI GCGC 3 cut(s) 365, 787, 1109
BstMAI GTCTC 3 cut(s) 389, 418, 1469
BstMCI CGRYCG 2 cut(s) 454, 563
BstNI CCWGG 4 cut(s) 144, 299, 671, 1733
BstNSI RCATGY 2 cut(s) 1000, 1614
BstSFI CTRYAG 1 cut(s) 1677
BstUI CGCG 6 cut(s) 360, 456, 527, 983, 1076, 1396
BstV1I GCAGC 7 cut(s) 231, 297, 357, 952, 1663, 1691, 1723
BstV2I GAAGAC 1 cut(s) 1045
BstXI CCANNNNNNTGG 1 cut(s) 226
BstZI CGGCCG 2 cut(s) 451, 560
BsuRI GGCC 9 cut(s) 147, 264, 453, 562, 824, 1267, 1628, 1643, 1662
BtgI CCRYGG 3 cut(s) 358, 622, 1198
BtgZI GCGATG 2 cut(s) 86, 1465
BtrI CACGTC 2 cut(s) 505, 1051
BtsCI GGATG 5 cut(s) 192, 1128, 1789, 1793, 1830
BtsI GCAGTG 1 cut(s) 1806
BtsIMutI CAGTG 3 cut(s) 320, 1656, 1806
BveI ACCTGC 1 cut(s) 934
Cac8I GCNNGC 2 cut(s) 497, 1384
CfoI GCGC 3 cut(s) 365, 787, 1109
Cfr42I CCGCGG 1 cut(s) 361
CpoI CGGWCCG 1 cut(s) 1095
CseI GACGC 7 cut(s) 482, 516, 562, 989, 1065, 1385, 1402
Csp6I GTAC 8 cut(s) 259, 328, 355, 715, 844, 1334, 1447, 1780
CspI CGGWCCG 1 cut(s) 1095
CviQI GTAC 8 cut(s) 259, 328, 355, 715, 844, 1334, 1447, 1780
DdeI CTNAG 4 cut(s) 596, 1599, 1720, 1802
DinI GGCGCC 2 cut(s) 364, 1108
DrdI GACNNNNNNGTC 1 cut(s) 434
DriI GACNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 434
EaeI YGGCCR 5 cut(s) 262, 451, 560, 1626, 1641
EagI CGGCCG 2 cut(s) 451, 560
Eam1104I CTCTTC 5 cut(s) 49, 141, 347, 353, 759
Eam1105I GACNNNNNGTC 1 cut(s) 439
EarI CTCTTC 5 cut(s) 49, 141, 347, 353, 759
EciI GGCGGA 6 cut(s) 859, 1053, 1074, 1218, 1377, 1435
Ecl136II GAGCTC 2 cut(s) 991, 1011
EclXI CGGCCG 2 cut(s) 451, 560
Eco130I CCWWGG 3 cut(s) 149, 1110, 1773
Eco24I GRGCYC 3 cut(s) 993, 1013, 1047
Eco31I GGTCTC 2 cut(s) 389, 1469
Eco47I GGWCC 7 cut(s) 153, 295, 537, 701, 737, 1061, 1095
Eco52I CGGCCG 2 cut(s) 451, 560
Eco53kI GAGCTC 2 cut(s) 991, 1011
Eco88I CYCGRG 2 cut(s) 179, 732
EcoICRI GAGCTC 2 cut(s) 991, 1011
EcoO109I RGGNCCY 1 cut(s) 537
EcoRI GAATTC 2 cut(s) 769, 863
EcoRII CCWGG 4 cut(s) 142, 297, 669, 1731
EcoT14I CCWWGG 3 cut(s) 149, 1110, 1773
EcoT38I GRGCYC 3 cut(s) 993, 1013, 1047
EgeI GGCGCC 2 cut(s) 364, 1108
EheI GGCGCC 2 cut(s) 364, 1108
ErhI CCWWGG 3 cut(s) 149, 1110, 1773
Esp3I CGTCTC 1 cut(s) 418
FaqI GGGAC 4 cut(s) 427, 523, 818, 1287
FauI CCCGC 3 cut(s) 249, 692, 712
FbaI TGATCA 4 cut(s) 189, 1234, 1345, 1407
FblI GTMKAC 1 cut(s) 426
FokI GGATG 5 cut(s) 199, 1135, 1776, 1780, 1837
FriOI GRGCYC 3 cut(s) 993, 1013, 1047
FspBI CTAG 2 cut(s) 1452, 1553
GlaI GCGC 3 cut(s) 364, 786, 1108
GsaI CCCAGC 1 cut(s) 1551
GsuI CTGGAG 3 cut(s) 102, 296, 997
HaeII RGCGCY 2 cut(s) 366, 1110
HaeIII GGCC 9 cut(s) 147, 264, 453, 562, 824, 1267, 1628, 1643, 1662
HgaI GACGC 7 cut(s) 482, 516, 562, 989, 1065, 1385, 1402
HhaI GCGC 3 cut(s) 365, 787, 1109
Hin1I GRCGYC 5 cut(s) 363, 435, 474, 554, 1107
Hin6I GCGC 3 cut(s) 363, 785, 1107
HinP1I GCGC 3 cut(s) 363, 785, 1107
HincII GTYRAC 2 cut(s) 427, 949
HindII GTYRAC 2 cut(s) 427, 949
HindIII AAGCTT 1 cut(s) 1424
HpaI GTTAAC 1 cut(s) 949
HphI GGTGA 4 cut(s) 154, 523, 565, 1213
Hpy166II GTNNAC 5 cut(s) 330, 427, 949, 1221, 1855
Hpy8I GTNNAC 5 cut(s) 330, 427, 949, 1221, 1855
Hpy99I CGWCG 9 cut(s) 27, 308, 437, 452, 476, 491, 494, 983, 1081
HpyAV CCTTC 5 cut(s) 62, 427, 528, 1028, 1222
HpyCH4III ACNGT 5 cut(s) 18, 793, 832, 962, 1875
HpyCH4IV ACGT 6 cut(s) 435, 504, 1050, 1217, 1445, 1702
HpyCH4V TGCA 4 cut(s) 318, 1637, 1679, 1739
HpyF3I CTNAG 4 cut(s) 596, 1599, 1720, 1802
HpySE526I ACGT 6 cut(s) 435, 504, 1050, 1217, 1445, 1702
Hsp92I GRCGYC 5 cut(s) 363, 435, 474, 554, 1107
HspAI GCGC 3 cut(s) 363, 785, 1107
KasI GGCGCC 2 cut(s) 362, 1106
Kpn2I TCCGGA 1 cut(s) 859
Ksp22I TGATCA 4 cut(s) 189, 1234, 1345, 1407
KspAI GTTAAC 1 cut(s) 949
KspI CCGCGG 1 cut(s) 361
LguI GCTCTTC 1 cut(s) 141
Lsp1109I GCAGC 7 cut(s) 231, 297, 357, 952, 1663, 1691, 1723
LweI GCATC 3 cut(s) 19, 327, 1534
MaeI CTAG 2 cut(s) 1452, 1553
MaeII ACGT 6 cut(s) 435, 504, 1050, 1217, 1445, 1702
MaeIII GTNAC 3 cut(s) 336, 793, 1178
MboII GAAGA 8 cut(s) 23, 36, 128, 134, 334, 340, 776, 1045
MhlI GDGCHC 4 cut(s) 312, 993, 1013, 1047
MluCI AATT 7 cut(s) 631, 694, 757, 769, 863, 1018, 1752
MluI ACGCGT 2 cut(s) 525, 1394
Mly113I GGCGCC 2 cut(s) 363, 1107
MlyI GAGTC 5 cut(s) 287, 422, 675, 736, 1361
MmeI TCCRAC 6 cut(s) 456, 546, 591, 764, 978, 1059
MroI TCCGGA 1 cut(s) 859
MseI TTAA 2 cut(s) 948, 1278
MspA1I CMGCKG 2 cut(s) 244, 360
MvaI CCWGG 4 cut(s) 144, 299, 671, 1733
MvnI CGCG 6 cut(s) 360, 456, 527, 983, 1076, 1396
NarI GGCGCC 2 cut(s) 363, 1107
NciI CCSGG 8 cut(s) 275, 392, 607, 805, 821, 848, 1630, 1664
NmeAIII GCCGAG 4 cut(s) 502, 538, 607, 1330
NmuCI GTSAC 1 cut(s) 1178
NspI RCATGY 2 cut(s) 1000, 1614
NspV TTCGAA 1 cut(s) 762
PaeR7I CTCGAG 1 cut(s) 179
PciI ACATGT 1 cut(s) 996
PciSI GCTCTTC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 5 cut(s) 177, 432, 453, 560, 705
PfeI GAWTC 6 cut(s) 237, 270, 610, 856, 973, 1650
PfoI TCCNGGA 3 cut(s) 273, 390, 605
PleI GAGTC 5 cut(s) 286, 422, 674, 736, 1360
PluTI GGCGCC 2 cut(s) 366, 1110
PpsI GAGTC 5 cut(s) 286, 422, 674, 736, 1360
Ppu21I YACGTR 2 cut(s) 1446, 1703
PpuMI RGGWCCY 1 cut(s) 537
PscI ACATGT 1 cut(s) 996
Psp124BI GAGCTC 2 cut(s) 993, 1013
Psp5II RGGWCCY 1 cut(s) 537
Psp6I CCWGG 4 cut(s) 142, 297, 669, 1731
PspFI CCCAGC 1 cut(s) 1547
PspGI CCWGG 4 cut(s) 142, 297, 669, 1731
PspPPI RGGWCCY 1 cut(s) 537
PspXI VCTCGAGB 1 cut(s) 179
PstI CTGCAG 1 cut(s) 1681
RsaI GTAC 8 cut(s) 260, 329, 356, 716, 845, 1335, 1448, 1781
RsaNI GTAC 8 cut(s) 259, 328, 355, 715, 844, 1334, 1447, 1780
Rsr2I CGGWCCG 1 cut(s) 1095
RsrII CGGWCCG 1 cut(s) 1095
SacI GAGCTC 2 cut(s) 993, 1013
SacII CCGCGG 1 cut(s) 361
SalI GTCGAC 1 cut(s) 425
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 2 cut(s) 948, 1278
ScaI AGTACT 1 cut(s) 1335
SchI GAGTC 5 cut(s) 287, 422, 675, 736, 1361
SduI GDGCHC 4 cut(s) 312, 993, 1013, 1047
SfaNI GCATC 3 cut(s) 19, 327, 1534
SfcI CTRYAG 1 cut(s) 1677
SfoI GGCGCC 2 cut(s) 364, 1108
Sfr274I CTCGAG 1 cut(s) 179
Sfr303I CCGCGG 1 cut(s) 361
SfuI TTCGAA 1 cut(s) 762
SgrBI CCGCGG 1 cut(s) 361
SinI GGWCC 7 cut(s) 153, 295, 537, 701, 737, 1061, 1095
SlaI CTCGAG 1 cut(s) 179
SmlI CTYRAG 2 cut(s) 179, 1746
SmoI CTYRAG 2 cut(s) 179, 1746
Sse9I AATT 7 cut(s) 631, 694, 757, 769, 863, 1018, 1752
SspDI GGCGCC 2 cut(s) 362, 1106
SspMI CTAG 2 cut(s) 1452, 1553
SstI GAGCTC 2 cut(s) 993, 1013
StyI CCWWGG 3 cut(s) 149, 1110, 1773
TaaI ACNGT 5 cut(s) 18, 793, 832, 962, 1875
TaiI ACGT 6 cut(s) 438, 507, 1053, 1220, 1448, 1705
TaqI TCGA 8 cut(s) 180, 351, 426, 447, 465, 762, 1298, 1526
TaqII GACCGA 1 cut(s) 1451
TasI AATT 7 cut(s) 631, 694, 757, 769, 863, 1018, 1752
TatI WGTACW 3 cut(s) 714, 1333, 1779
TauI GCSGC 8 cut(s) 108, 363, 404, 456, 937, 940, 1076, 1362
TfiI GAWTC 6 cut(s) 237, 270, 610, 856, 973, 1650
Tru1I TTAA 2 cut(s) 948, 1278
Tru9I TTAA 2 cut(s) 948, 1278
TscAI CASTG 3 cut(s) 320, 1663, 1813
TseFI GTSAC 1 cut(s) 1178
TseI GCWGC 7 cut(s) 244, 285, 370, 940, 1676, 1679, 1736
Tsp45I GTSAC 1 cut(s) 1178
TspDTI ATGAA 4 cut(s) 50, 89, 102, 1778
TspGWI ACGGA 1 cut(s) 186
TspRI CASTG 3 cut(s) 320, 1663, 1813
VpaK11BI GGWCC 7 cut(s) 153, 295, 537, 701, 737, 1061, 1095
XapI RAATTY 2 cut(s) 769, 863
XceI RCATGY 2 cut(s) 1000, 1614
XcmI CCANNNNNNNNNTGG 1 cut(s) 1145
XhoI CTCGAG 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 426
XspI CTAG 2 cut(s) 1452, 1553
ZraI GACGTC 1 cut(s) 436
ZrmI AGTACT 1 cut(s) 1335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.