FvH4_5g13320

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001496
Physical Location & Seq
Forward (+)
7515776 .. 7517253
1478 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13320.t1

Sequence Viewer

Length: 1311 bp
ATGGTGTCGCAAAACCTGGCTTCCTTTAAATCCAGGCAAGAGTATCGCAGGAACGTCGAATTGGAAGAAGCGCGTAAGGCTGGGCTTGTACCTGCTGAAGTCGATAAGAATGGAAAGGAGATCAATCCTCATATACCCAAATATGTGTCTGATACACCTTGGTATATCAAAACCGACGAGGGACCAAGTCTAGAGCATCAAAGAAAGCCAGAATCAAACGTGAATGGCAAGAAGAAATCTCAGTATGACAGTGGTGCCAAGGTAATCCAGGCCCTTAAATATAGGGAGGGTGCATGTACAAACTGTGGATCAATGAAACATGATGCCAAGTCTTGCATTGAAAGACCGAGGAAAGTTGGAGCAAAATGGAACGACAAGCACATTGCCCCGGATGAGATTGTAGAGACCCTTGGACATCTCACCTATGATGAAAAGCGTGACCGTTGGGATGGATACAATCCATCAAGCTATGCTCATGTTCTTAAGAGATATGAATTGCAACAGGAAGCTCGAAAAAAGTACTTGAAGGAAGAAAGAATCAAGAAATTAGAAGATCAAGATGTTCATCATGATGATGATATGAGGGTGGATGAAGCCAAGGTTGATGAGAGCAAGCAAACGGACTTCGCAAAGGTTGAGAAGCGTGTGCGTACAGCGGGTGGTGGAAGCACTGGAACTGTTAGGAACTTACGTATCCGTGAAGACACGGCAAAATATCTTCGGAATCTTGATGTCAACTCTGCGCATTATGATCCGAAAAGCCGATCCATGCGTGAGAATCCTAATCCGGACGCAGATCCTAACGAGCAGTTCTATGCAGGTGATAATGTATGGAGAAACACTGGTCAGGCTCTGGATTTTAACCAGATGAACATGCATGCTTTGGAAGCATTTGAGAGGGGACAAGATGTACACATGCAAGCAGCTCCATCGCAAGCTGAGTTTCTATACAAAAATTACAAGAAAATCAAGGAGAGATTTACTACGCAGACCAATTGCACCGTTCTAGAAAAATATGGCAATGCAGCCAGTGGAGGAGAACTTCCTAAGGAGCTTCTACTGGGACAGAGTGAACGAGTTGTCGAATACGATCGAGCTGGGAGGATCATTAAGGGCGAGGAAGAAGCGGTTCCTAGGAGCAAGTATGCAGAAGATGTCATTATCAACAACCACACTTGTGTGTGGGGTTCGTGGTGGAAAGGTCACCGATGGGGGTACAAGTGTTGTAAGCAATTTATGCGAAACAGTTATTGCACAGGTGCCGCTGGAGTTGAGGCTGCCGAGGCTGCAATACATCTCATGGAGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Slu7

Protein Analysis

437

Amino Acids

50.05

Weight (kDa)

8.22

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Slu7 PF11708 140 - 397 2.4e-89 Pre-mRNA splicing Prp18-interacting factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 809
Acc16I TGCGCA 1 cut(s) 744
Acc36I ACCTGC 2 cut(s) 100, 809
AccB1I GGYRCC 2 cut(s) 254, 1259
AccII CGCG 1 cut(s) 73
AccIII TCCGGA 1 cut(s) 787
AciI CCGC 3 cut(s) 656, 1127, 1263
AclWI GGATC 5 cut(s) 316, 746, 759, 791, 1112
AcuI CTGAAG 1 cut(s) 117
AfaI GTAC 6 cut(s) 90, 298, 521, 652, 912, 1217
AflII CTTAAG 1 cut(s) 482
AgsI TTSAA 2 cut(s) 341, 526
AjnI CCWGG 3 cut(s) 15, 32, 267
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AleI CACNNNNGTG 2 cut(s) 1176, 1178
AluBI AGCT 6 cut(s) 468, 509, 926, 938, 1054, 1097
AluI AGCT 6 cut(s) 468, 509, 926, 938, 1054, 1097
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 5 cut(s) 316, 746, 759, 791, 1112
AlwNI CAGNNNCTG 1 cut(s) 853
Aor13HI TCCGGA 1 cut(s) 787
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 4 cut(s) 923, 1025, 1277, 1286
Asp700I GAANNNNTTC 1 cut(s) 1128
AspA2I CCTAGG 1 cut(s) 1133
AspLEI GCGC 2 cut(s) 73, 745
AspS9I GGNCC 2 cut(s) 182, 271
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 3 cut(s) 412, 833, 1196
AvaII GGWCC 1 cut(s) 182
AvrII CCTAGG 1 cut(s) 1133
AxyI CCTNAGG 1 cut(s) 1047
BaeI ACNNNNGTAYC 4 cut(s) 676, 709, 1207, 1240
BanI GGYRCC 2 cut(s) 254, 1259
BbsI GAAGAC 1 cut(s) 708
BbvI GCAGC 4 cut(s) 935, 1037, 1264, 1273
BccI CCATC 4 cut(s) 443, 469, 937, 1203
BceAI ACGGC 1 cut(s) 723
BcgI CGANNNNNNTGC 6 cut(s) 26, 37, 60, 71, 912, 946
BciT130I CCWGG 3 cut(s) 17, 34, 269
BciVI GTATCC 2 cut(s) 446, 704
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 3 cut(s) 191, 1007, 1134
BfrI CTTAAG 1 cut(s) 482
BfuAI ACCTGC 2 cut(s) 100, 809
BfuI GTATCC 2 cut(s) 446, 704
BisI GCNGC 5 cut(s) 924, 1026, 1263, 1278, 1287
BlnI CCTAGG 1 cut(s) 1133
BlsI GCNGC 5 cut(s) 925, 1027, 1264, 1279, 1288
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 4 cut(s) 17, 34, 269, 389
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 2 cut(s) 182, 271
BmiI GGNNCC 5 cut(s) 183, 256, 1131, 1261, 1305
BmrFI CCNGG 4 cut(s) 17, 34, 269, 389
BmrI ACTGGG 1 cut(s) 1070
BmsI GCATC 2 cut(s) 205, 313
BmuI ACTGGG 1 cut(s) 1070
BpiI GAAGAC 1 cut(s) 708
BpmI CTGGAG 1 cut(s) 1287
BpuMI CCSGG 1 cut(s) 389
BsaAI YACGTR 1 cut(s) 692
BsaBI GATNNNNATC 1 cut(s) 564
BsaI GGTCTC 1 cut(s) 398
BsaJI CCNNGG 8 cut(s) 158, 258, 347, 387, 409, 597, 1133, 1281
BsaWI WCCGGW 1 cut(s) 787
Bse1I ACTGG 4 cut(s) 676, 847, 1029, 1065
Bse21I CCTNAGG 1 cut(s) 1047
Bse3DI GCAATG 2 cut(s) 381, 1027
Bse8I GATNNNNATC 1 cut(s) 564
BseAI TCCGGA 1 cut(s) 787
BseBI CCWGG 3 cut(s) 17, 34, 269
BseDI CCNNGG 8 cut(s) 158, 258, 347, 387, 409, 597, 1133, 1281
BseGI GGATG 3 cut(s) 397, 454, 595
BseJI GATNNNNATC 1 cut(s) 564
BseMI GCAATG 2 cut(s) 381, 1027
BseMII CTCAG 2 cut(s) 254, 930
BseNI ACTGG 4 cut(s) 676, 847, 1029, 1065
BseRI GAGGAG 1 cut(s) 1050
BseXI GCAGC 4 cut(s) 935, 1037, 1264, 1273
BseYI CCCAGC 2 cut(s) 80, 1097
Bsh1236I CGCG 1 cut(s) 73
Bsh1285I CGRYCG 1 cut(s) 1093
BshFI GGCC 1 cut(s) 272
BshNI GGYRCC 2 cut(s) 254, 1259
BsiEI CGRYCG 1 cut(s) 1093
BsiSI CCGG 2 cut(s) 389, 788
BslFI GGGAC 3 cut(s) 195, 915, 1077
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 3 cut(s) 195, 915, 1077
BsnI GGCC 1 cut(s) 272
Bso31I GGTCTC 1 cut(s) 398
Bsp13I TCCGGA 1 cut(s) 787
Bsp1407I TGTACA 2 cut(s) 296, 910
Bsp143I GATC 8 cut(s) 120, 308, 553, 751, 764, 796, 1090, 1104
BspACI CCGC 3 cut(s) 656, 1127, 1263
BspANI GGCC 1 cut(s) 272
BspCNI CTCAG 2 cut(s) 253, 931
BspEI TCCGGA 1 cut(s) 787
BspFNI CGCG 1 cut(s) 73
BspHI TCATGA 1 cut(s) 568
BspLI GGNNCC 5 cut(s) 183, 256, 1131, 1261, 1305
BspMI ACCTGC 2 cut(s) 100, 809
BspPI GGATC 5 cut(s) 316, 746, 759, 791, 1112
BspT107I GGYRCC 2 cut(s) 254, 1259
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 398
BsrDI GCAATG 2 cut(s) 381, 1027
BsrGI TGTACA 2 cut(s) 296, 910
BsrI ACTGG 4 cut(s) 676, 847, 1029, 1065
BssECI CCNNGG 8 cut(s) 158, 258, 347, 387, 409, 597, 1133, 1281
BssMI GATC 8 cut(s) 120, 308, 553, 751, 764, 796, 1090, 1104
BssT1I CCWWGG 5 cut(s) 158, 258, 409, 597, 1133
Bst2UI CCWGG 3 cut(s) 17, 34, 269
Bst4CI ACNGT 6 cut(s) 251, 305, 443, 679, 1003, 1247
BstAFI CTTAAG 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 1237
BstAUI TGTACA 2 cut(s) 296, 910
BstBAI YACGTR 1 cut(s) 692
BstC8I GCNNGC 4 cut(s) 614, 879, 921, 936
BstDEI CTNAG 3 cut(s) 240, 939, 1047
BstEII GGTNACC 1 cut(s) 1202
BstF5I GGATG 3 cut(s) 397, 454, 595
BstFNI CGCG 1 cut(s) 73
BstHHI GCGC 2 cut(s) 73, 745
BstKTI GATC 8 cut(s) 123, 311, 556, 754, 767, 799, 1093, 1107
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 8 cut(s) 120, 308, 553, 751, 764, 796, 1090, 1104
BstMCI CGRYCG 1 cut(s) 1093
BstMWI GCNNNNNNNGC 5 cut(s) 77, 887, 1237, 1283, 1286
BstNI CCWGG 3 cut(s) 17, 34, 269
BstNSI RCATGY 4 cut(s) 297, 877, 881, 919
BstPI GGTNACC 1 cut(s) 1202
BstSCI CCNGG 4 cut(s) 15, 32, 267, 387
BstSNI TACGTA 1 cut(s) 692
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 4 cut(s) 935, 1037, 1264, 1273
BstV2I GAAGAC 1 cut(s) 708
BstX2I RGATCY 1 cut(s) 796
BstYI RGATCY 1 cut(s) 796
Bsu36I CCTNAGG 1 cut(s) 1047
BsuI GTATCC 2 cut(s) 446, 704
BsuRI GGCC 1 cut(s) 272
BtgZI GCGATG 1 cut(s) 915
BtsCI GGATG 3 cut(s) 397, 454, 595
BtsIMutI CAGTG 4 cut(s) 256, 669, 840, 1036
BveI ACCTGC 2 cut(s) 100, 809
Cac8I GCNNGC 4 cut(s) 614, 879, 921, 936
CaiI CAGNNNCTG 1 cut(s) 853
CciI TCATGA 1 cut(s) 568
CfoI GCGC 2 cut(s) 73, 745
Cfr13I GGNCC 2 cut(s) 182, 271
CseI GACGC 1 cut(s) 800
Csp6I GTAC 6 cut(s) 89, 297, 520, 651, 911, 1216
CviAII CATG 9 cut(s) 294, 320, 476, 569, 769, 874, 878, 916, 1300
CviQI GTAC 6 cut(s) 89, 297, 520, 651, 911, 1216
DdeI CTNAG 3 cut(s) 240, 939, 1047
DpnI GATC 8 cut(s) 122, 310, 555, 753, 766, 798, 1092, 1106
DpnII GATC 8 cut(s) 120, 308, 553, 751, 764, 796, 1090, 1104
DraI TTTAAA 1 cut(s) 28
Eco105I TACGTA 1 cut(s) 692
Eco130I CCWWGG 5 cut(s) 158, 258, 409, 597, 1133
Eco31I GGTCTC 1 cut(s) 398
Eco47I GGWCC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 117
Eco81I CCTNAGG 1 cut(s) 1047
Eco91I GGTNACC 1 cut(s) 1202
EcoO109I RGGNCCY 1 cut(s) 271
EcoO65I GGTNACC 1 cut(s) 1202
EcoRII CCWGG 3 cut(s) 15, 32, 267
EcoT14I CCWWGG 5 cut(s) 158, 258, 409, 597, 1133
EcoT22I ATGCAT 1 cut(s) 879
ErhI CCWWGG 5 cut(s) 158, 258, 409, 597, 1133
FaeI CATG 9 cut(s) 297, 323, 479, 572, 772, 877, 881, 919, 1303
FaqI GGGAC 3 cut(s) 195, 915, 1077
FatI CATG 9 cut(s) 293, 319, 475, 568, 768, 873, 877, 915, 1299
FauI CCCGC 1 cut(s) 649
Fnu4HI GCNGC 5 cut(s) 924, 1026, 1263, 1278, 1287
FokI GGATG 3 cut(s) 404, 461, 602
Fsp4HI GCNGC 5 cut(s) 924, 1026, 1263, 1278, 1287
FspBI CTAG 3 cut(s) 191, 1007, 1134
FspI TGCGCA 1 cut(s) 744
GlaI GCGC 2 cut(s) 72, 744
GluI GCNGC 5 cut(s) 924, 1026, 1263, 1278, 1287
GsaI CCCAGC 2 cut(s) 84, 1101
GsuI CTGGAG 1 cut(s) 1287
HaeIII GGCC 1 cut(s) 272
HapII CCGG 2 cut(s) 389, 788
HgaI GACGC 1 cut(s) 800
HhaI GCGC 2 cut(s) 73, 745
Hin1II CATG 9 cut(s) 297, 323, 479, 572, 772, 877, 881, 919, 1303
Hin6I GCGC 2 cut(s) 71, 743
HinP1I GCGC 2 cut(s) 71, 743
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HinfI GANTC 4 cut(s) 212, 537, 724, 778
HpaII CCGG 2 cut(s) 389, 788
HphI GGTGA 3 cut(s) 412, 833, 1196
Hpy166II GTNNAC 3 cut(s) 736, 913, 1073
Hpy188I TCNGA 3 cut(s) 151, 723, 756
Hpy188III TCNNGA 8 cut(s) 191, 541, 557, 569, 728, 788, 854, 1007
Hpy8I GTNNAC 3 cut(s) 736, 913, 1073
Hpy99I CGWCG 2 cut(s) 59, 179
HpyAV CCTTC 1 cut(s) 520
HpyCH4III ACNGT 6 cut(s) 251, 305, 443, 679, 1003, 1247
HpyCH4IV ACGT 3 cut(s) 54, 219, 691
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 887, 1237, 1283, 1286
HpyF3I CTNAG 3 cut(s) 240, 939, 1047
HpySE526I ACGT 3 cut(s) 54, 219, 691
Hsp92II CATG 9 cut(s) 297, 323, 479, 572, 772, 877, 881, 919, 1303
HspAI GCGC 2 cut(s) 71, 743
Kpn2I TCCGGA 1 cut(s) 787
Kzo9I GATC 8 cut(s) 120, 308, 553, 751, 764, 796, 1090, 1104
LmnI GCTCC 5 cut(s) 359, 931, 1051, 1137, 1303
Lsp1109I GCAGC 4 cut(s) 935, 1037, 1264, 1273
LweI GCATC 2 cut(s) 205, 313
MaeI CTAG 3 cut(s) 191, 1007, 1134
MaeII ACGT 3 cut(s) 54, 219, 691
MaeIII GTNAC 2 cut(s) 437, 1202
MalI GATC 8 cut(s) 122, 310, 555, 753, 766, 798, 1092, 1106
MboI GATC 8 cut(s) 120, 308, 553, 751, 764, 796, 1090, 1104
MboII GAAGA 8 cut(s) 77, 244, 542, 563, 710, 713, 1133, 1163
MfeI CAATTG 1 cut(s) 994
MflI RGATCY 1 cut(s) 796
MluCI AATT 6 cut(s) 59, 494, 545, 955, 994, 1232
MmeI TCCRAC 1 cut(s) 337
Mph1103I ATGCAT 1 cut(s) 879
MroI TCCGGA 1 cut(s) 787
MroXI GAANNNNTTC 1 cut(s) 1128
MseI TTAA 6 cut(s) 27, 276, 483, 861, 1110, 1309
MslI CAYNNNNRTG 4 cut(s) 570, 573, 1176, 1178
MspA1I CMGCKG 2 cut(s) 656, 1265
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 2 cut(s) 389, 788
MspR9I CCNGG 4 cut(s) 17, 34, 269, 389
MunI CAATTG 1 cut(s) 994
MvaI CCWGG 3 cut(s) 17, 34, 269
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 5 cut(s) 77, 887, 1237, 1283, 1286
NciI CCSGG 1 cut(s) 389
NdeII GATC 8 cut(s) 120, 308, 553, 751, 764, 796, 1090, 1104
NlaIII CATG 9 cut(s) 297, 323, 479, 572, 772, 877, 881, 919, 1303
NlaIV GGNNCC 5 cut(s) 183, 256, 1131, 1261, 1305
NmeAIII GCCGAG 1 cut(s) 1306
NmuCI GTSAC 2 cut(s) 437, 1202
NsbI TGCGCA 1 cut(s) 744
NsiI ATGCAT 1 cut(s) 879
NspI RCATGY 4 cut(s) 297, 877, 881, 919
OliI CACNNNNGTG 2 cut(s) 1176, 1178
PaeI GCATGC 1 cut(s) 881
PagI TCATGA 1 cut(s) 568
PaqCI CACCTGC 1 cut(s) 809
PdmI GAANNNNTTC 1 cut(s) 1128
PfeI GAWTC 4 cut(s) 212, 537, 724, 778
PflFI GACNNNGTC 1 cut(s) 186
PkrI GCNGC 5 cut(s) 925, 1027, 1264, 1279, 1288
Ple19I CGATCG 1 cut(s) 1093
Ppu21I YACGTR 1 cut(s) 692
Psp6I CCWGG 3 cut(s) 15, 32, 267
PspEI GGTNACC 1 cut(s) 1202
PspFI CCCAGC 2 cut(s) 80, 1097
PspGI CCWGG 3 cut(s) 15, 32, 267
PspN4I GGNNCC 5 cut(s) 183, 256, 1131, 1261, 1305
PspPI GGNCC 2 cut(s) 182, 271
PstNI CAGNNNCTG 1 cut(s) 853
PsuI RGATCY 1 cut(s) 796
PsyI GACNNNGTC 1 cut(s) 186
PvuI CGATCG 1 cut(s) 1093
RsaI GTAC 6 cut(s) 90, 298, 521, 652, 912, 1217
RsaNI GTAC 6 cut(s) 89, 297, 520, 651, 911, 1216
RseI CAYNNNNRTG 4 cut(s) 570, 573, 1176, 1178
SaqAI TTAA 6 cut(s) 27, 276, 483, 861, 1110, 1309
SatI GCNGC 5 cut(s) 924, 1026, 1263, 1278, 1287
Sau3AI GATC 8 cut(s) 120, 308, 553, 751, 764, 796, 1090, 1104
Sau96I GGNCC 2 cut(s) 182, 271
ScaI AGTACT 1 cut(s) 521
ScrFI CCNGG 4 cut(s) 17, 34, 269, 389
SfaNI GCATC 2 cut(s) 205, 313
SinI GGWCC 1 cut(s) 182
SmiMI CAYNNNNRTG 4 cut(s) 570, 573, 1176, 1178
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SnaBI TACGTA 1 cut(s) 692
SphI GCATGC 1 cut(s) 881
Sse9I AATT 6 cut(s) 59, 494, 545, 955, 994, 1232
SsiI CCGC 3 cut(s) 656, 1127, 1263
SspMI CTAG 3 cut(s) 191, 1007, 1134
StyD4I CCNGG 4 cut(s) 15, 32, 267, 387
StyI CCWWGG 5 cut(s) 158, 258, 409, 597, 1133
TaaI ACNGT 6 cut(s) 251, 305, 443, 679, 1003, 1247
TaiI ACGT 3 cut(s) 57, 222, 694
TaqI TCGA 5 cut(s) 57, 102, 511, 1083, 1093
TaqII GACCGA 1 cut(s) 361
TasI AATT 6 cut(s) 59, 494, 545, 955, 994, 1232
TatI WGTACW 3 cut(s) 296, 519, 910
TauI GCSGC 1 cut(s) 1265
TfiI GAWTC 4 cut(s) 212, 537, 724, 778
Tru1I TTAA 6 cut(s) 27, 276, 483, 861, 1110, 1309
Tru9I TTAA 6 cut(s) 27, 276, 483, 861, 1110, 1309
TscAI CASTG 4 cut(s) 256, 676, 847, 1036
TseFI GTSAC 2 cut(s) 437, 1202
TseI GCWGC 4 cut(s) 923, 1025, 1277, 1286
Tsp45I GTSAC 2 cut(s) 437, 1202
TspDTI ATGAA 6 cut(s) 329, 444, 507, 554, 606, 884
TspGWI ACGGA 2 cut(s) 635, 686
TspRI CASTG 4 cut(s) 256, 676, 847, 1036
Tth111I GACNNNGTC 1 cut(s) 186
Vha464I CTTAAG 1 cut(s) 482
VpaK11BI GGWCC 1 cut(s) 182
XbaI TCTAGA 2 cut(s) 190, 1006
XceI RCATGY 4 cut(s) 297, 877, 881, 919
XmaJI CCTAGG 1 cut(s) 1133
XmnI GAANNNNTTC 1 cut(s) 1128
XspI CTAG 3 cut(s) 191, 1007, 1134
ZrmI AGTACT 1 cut(s) 521
Zsp2I ATGCAT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.