pycom05g18440

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001496
Physical Location & Seq
Reverse (-)
21360432 .. 21362208
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18440.5

Sequence Viewer

Length: 1125 bp
ATGTCATCTGCTCCTTGGTATCTCAAACACGAGAGGCCTAGCTTGAAACATCAAAGGAAATGGAAATCAGATCCAAATTACACAACATCATGGTATGACAGAGGTGCAAAGATATTCCAGGCTGATAAATATAGAAAGGGTGCATGTGAGAATTGTGGTGCCAGTACACACGACTCAAAGTCATGCATGGATAGGCCCAGGAAAGTGGGAGCAAAGTGGACAAACACACACATTGCTCCTGATGAGAAGATAGAGACTTTTGAGCTTGACTATGATGGAAAGCGGGACCGCTGGAATGGATATGATGCAACGAGCTATGCTCTTGTAGTAGAGAGATATGAATCAAGAGATGAAGCTCGAAAGAAATTCTTAAAGGAACAGCAACTCAAGAAGTTAGAGGAGAAAAATAATAATCCAAATGGCGAGGACAAGGTTAGTGATGTGGATGAAGATGAAGACGAGGATGAGGATGATTTGAGGGTAGATGAAGCTAAGGTTGATGAGAGTAAACAAATGGACTTTGCAAAGGTTGAGAAGCGTGTACGTACAACTGGTGGTGGAAGCACAGGAACGGTCAGGAATTTGCGTATTCGTGAGGATACAGCGAAATATCTTTTGAACCTCGATGTCAACTCTGCACATTATGACCCCAAAACCCGTTCTATGCGTGAAGACCCTCTTCCAGATGCTGATCCGAATGAGAAGTTTTACGGGGGTGATAATCGATATAGAAATAGTGGTCAAGCCATGGAGTTCAAGGAGCTCAACATCCATGCTTGGGAAGCATTTGATAAGGGACAAGATATCCACATGCAAGCAGCGCCTTCCCAGGCCGAGTTGCTTTATAAGAATTATCAGGTGATCAAGGAGAATTTGAAGTCAAAAACAAAAGACACTGTTCTGGAGAAGTACGGCAATGCAGCTAATGAAGAAGAATTTCCTAGGGAGCTTCTACTGGGACAAAGTGAAAGGGAAGTTGAATATGATCGTGCTGGGAGAATCATCAAGGGCCAGGACATAGCACTTCCCAGAAGCAAGTATGAAGAAGATGTCTATGTTAATAACCACACCACTGTATGGGGTTCATGGTGGAAGGATCGATCAATGGGGCTACAGGTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Slu7

Protein Analysis

375

Amino Acids

43.58

Weight (kDa)

5.6

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 846
AccB1I GGYRCC 1 cut(s) 158
AccB7I CCANNNNNTGG 1 cut(s) 1077
AciI CCGC 2 cut(s) 283, 289
AclWI GGATC 3 cut(s) 65, 686, 1104
AcsI RAATTY 4 cut(s) 365, 580, 871, 935
AfaI GTAC 4 cut(s) 166, 543, 547, 911
AfiI CCNNNNNNNGG 2 cut(s) 778, 1077
AgsI TTSAA 5 cut(s) 46, 619, 757, 877, 980
AhdI GACNNNNNGTC 1 cut(s) 178
AjnI CCWGG 4 cut(s) 117, 197, 828, 1011
AluBI AGCT 8 cut(s) 42, 265, 315, 356, 491, 763, 923, 949
AluI AGCT 8 cut(s) 42, 265, 315, 356, 491, 763, 923, 949
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 1 cut(s) 248
AlwI GGATC 3 cut(s) 65, 686, 1104
AlwNI CAGNNNCTG 1 cut(s) 689
AoxI GGCC 4 cut(s) 35, 194, 831, 1009
ApeKI GCWGC 2 cut(s) 818, 920
ApoI RAATTY 4 cut(s) 365, 580, 871, 935
Asp700I GAANNNNTTC 1 cut(s) 936
AspA2I CCTAGG 1 cut(s) 941
AspLEI GCGC 1 cut(s) 823
AspS9I GGNCC 3 cut(s) 195, 286, 1009
AsuHPI GGTGA 2 cut(s) 728, 871
AvaII GGWCC 1 cut(s) 286
AvrII CCTAGG 1 cut(s) 941
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 765
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 2 cut(s) 462, 678
Bbv12I GWGCWC 1 cut(s) 765
BbvI GCAGC 2 cut(s) 830, 932
BccI CCATC 1 cut(s) 269
BceAI ACGGC 1 cut(s) 928
BciT130I CCWGG 4 cut(s) 119, 199, 830, 1013
BciVI GTATCC 1 cut(s) 592
BclI TGATCA 1 cut(s) 861
BcoDI GTCTC 1 cut(s) 248
BfaI CTAG 2 cut(s) 39, 942
BfmI CTRYAG 1 cut(s) 1112
BfoI RGCGCY 1 cut(s) 824
BfuI GTATCC 1 cut(s) 592
BisI GCNGC 2 cut(s) 819, 921
BlnI CCTAGG 1 cut(s) 941
BlsI GCNGC 2 cut(s) 820, 922
Bme1390I CCNGG 4 cut(s) 119, 199, 830, 1013
Bme18I GGWCC 1 cut(s) 286
BmeRI GACNNNNNGTC 1 cut(s) 178
BmgT120I GGNCC 3 cut(s) 195, 286, 1009
BmiI GGNNCC 2 cut(s) 160, 287
BmrFI CCNGG 4 cut(s) 119, 199, 830, 1013
BmrI ACTGGG 1 cut(s) 965
BmsI GCATC 2 cut(s) 295, 676
BmuI ACTGGG 1 cut(s) 965
BpiI GAAGAC 2 cut(s) 462, 678
BplI GAGNNNNNCTC 2 cut(s) 304, 336
BpmI CTGGAG 1 cut(s) 923
Bpu10I CCTNAGC 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 371
Bsa29I ATCGAT 2 cut(s) 724, 1099
BsaAI YACGTR 1 cut(s) 545
BsaBI GATNNNNATC 2 cut(s) 340, 690
BsaJI CCNNGG 5 cut(s) 14, 197, 747, 828, 941
Bsc4I CCNNNNNNNGG 2 cut(s) 778, 1077
Bse1I ACTGG 3 cut(s) 162, 556, 960
Bse3DI GCAATG 2 cut(s) 231, 922
Bse8I GATNNNNATC 2 cut(s) 340, 690
BseBI CCWGG 4 cut(s) 119, 199, 830, 1013
BseCI ATCGAT 2 cut(s) 724, 1099
BseDI CCNNGG 5 cut(s) 14, 197, 747, 828, 941
BseGI GGATG 4 cut(s) 451, 469, 475, 768
BseJI GATNNNNATC 2 cut(s) 340, 690
BseLI CCNNNNNNNGG 2 cut(s) 778, 1077
BseMI GCAATG 2 cut(s) 231, 922
BseNI ACTGG 3 cut(s) 162, 556, 960
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 2 cut(s) 830, 932
BseYI CCCAGC 1 cut(s) 992
BsgI GTGCAG 1 cut(s) 621
BshFI GGCC 4 cut(s) 37, 196, 833, 1011
BshNI GGYRCC 1 cut(s) 158
BshVI ATCGAT 2 cut(s) 724, 1099
BsiHKAI GWGCWC 1 cut(s) 765
BslFI GGGAC 3 cut(s) 299, 810, 972
BslI CCNNNNNNNGG 2 cut(s) 778, 1077
BsmAI GTCTC 1 cut(s) 248
BsmFI GGGAC 3 cut(s) 299, 810, 972
BsnI GGCC 4 cut(s) 37, 196, 833, 1011
Bsp1286I GDGCHC 1 cut(s) 765
Bsp143I GATC 6 cut(s) 70, 691, 861, 985, 1096, 1100
Bsp19I CCATGG 1 cut(s) 747
BspACI CCGC 2 cut(s) 283, 289
BspANI GGCC 4 cut(s) 37, 196, 833, 1011
BspDI ATCGAT 2 cut(s) 724, 1099
BspLI GGNNCC 2 cut(s) 160, 287
BspPI GGATC 3 cut(s) 65, 686, 1104
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 2 cut(s) 231, 922
BsrI ACTGG 3 cut(s) 162, 556, 960
BssECI CCNNGG 5 cut(s) 14, 197, 747, 828, 941
BssMI GATC 6 cut(s) 70, 691, 861, 985, 1096, 1100
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 3 cut(s) 14, 747, 941
Bst2BI CACGAG 1 cut(s) 29
Bst2UI CCWGG 4 cut(s) 119, 199, 830, 1013
Bst4CI ACNGT 3 cut(s) 574, 898, 1075
Bst6I CTCTTC 1 cut(s) 684
BstBAI YACGTR 1 cut(s) 545
BstC8I GCNNGC 1 cut(s) 816
BstDEI CTNAG 1 cut(s) 492
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 4 cut(s) 451, 469, 475, 768
BstH2I RGCGCY 1 cut(s) 824
BstHHI GCGC 1 cut(s) 823
BstKTI GATC 6 cut(s) 73, 694, 864, 988, 1099, 1103
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 6 cut(s) 70, 691, 861, 985, 1096, 1100
BstMWI GCNNNNNNNGC 2 cut(s) 782, 820
BstNI CCWGG 4 cut(s) 119, 199, 830, 1013
BstNSI RCATGY 2 cut(s) 147, 814
BstSCI CCNGG 4 cut(s) 117, 197, 828, 1011
BstSFI CTRYAG 1 cut(s) 1112
BstSNI TACGTA 1 cut(s) 545
BstV1I GCAGC 2 cut(s) 830, 932
BstV2I GAAGAC 2 cut(s) 462, 678
BstX2I RGATCY 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 1 cut(s) 70
Bsu15I ATCGAT 2 cut(s) 724, 1099
BsuI GTATCC 1 cut(s) 592
BsuRI GGCC 4 cut(s) 37, 196, 833, 1011
BsuTUI ATCGAT 2 cut(s) 724, 1099
BtgI CCRYGG 1 cut(s) 747
BtsCI GGATG 4 cut(s) 451, 469, 475, 768
BtsIMutI CAGTG 2 cut(s) 894, 1071
Cac8I GCNNGC 1 cut(s) 816
CaiI CAGNNNCTG 1 cut(s) 689
CfoI GCGC 1 cut(s) 823
Cfr13I GGNCC 3 cut(s) 195, 286, 1009
ClaI ATCGAT 2 cut(s) 724, 1099
Csp6I GTAC 4 cut(s) 165, 542, 546, 910
CviAII CATG 8 cut(s) 90, 144, 183, 187, 748, 773, 811, 1086
CviQI GTAC 4 cut(s) 165, 542, 546, 910
DdeI CTNAG 1 cut(s) 492
DpnI GATC 6 cut(s) 72, 693, 863, 987, 1098, 1102
DpnII GATC 6 cut(s) 70, 691, 861, 985, 1096, 1100
DriI GACNNNNNGTC 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 684
Eam1105I GACNNNNNGTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 684
Ecl136II GAGCTC 1 cut(s) 763
Eco105I TACGTA 1 cut(s) 545
Eco130I CCWWGG 3 cut(s) 14, 747, 941
Eco147I AGGCCT 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 765
Eco32I GATATC 1 cut(s) 805
Eco47I GGWCC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 763
EcoICRI GAGCTC 1 cut(s) 763
EcoRII CCWGG 4 cut(s) 117, 197, 828, 1011
EcoRV GATATC 1 cut(s) 805
EcoT14I CCWWGG 3 cut(s) 14, 747, 941
EcoT22I ATGCAT 1 cut(s) 188
EcoT38I GRGCYC 1 cut(s) 765
ErhI CCWWGG 3 cut(s) 14, 747, 941
FaeI CATG 8 cut(s) 93, 147, 186, 190, 751, 776, 814, 1089
FalI AAGNNNNNCTT 4 cut(s) 353, 385, 663, 695
FaqI GGGAC 3 cut(s) 299, 810, 972
FatI CATG 8 cut(s) 89, 143, 182, 186, 747, 772, 810, 1085
FauI CCCGC 1 cut(s) 276
FbaI TGATCA 1 cut(s) 861
Fnu4HI GCNGC 2 cut(s) 819, 921
FokI GGATG 4 cut(s) 458, 476, 482, 755
FriOI GRGCYC 1 cut(s) 765
Fsp4HI GCNGC 2 cut(s) 819, 921
FspBI CTAG 2 cut(s) 39, 942
GlaI GCGC 1 cut(s) 822
GluI GCNGC 2 cut(s) 819, 921
GsaI CCCAGC 1 cut(s) 996
GsuI CTGGAG 1 cut(s) 923
HaeII RGCGCY 1 cut(s) 824
HaeIII GGCC 4 cut(s) 37, 196, 833, 1011
HhaI GCGC 1 cut(s) 823
Hin1II CATG 8 cut(s) 93, 147, 186, 190, 751, 776, 814, 1089
Hin6I GCGC 1 cut(s) 821
HinP1I GCGC 1 cut(s) 821
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 3 cut(s) 173, 341, 999
HphI GGTGA 2 cut(s) 728, 871
Hpy166II GTNNAC 5 cut(s) 167, 219, 509, 542, 631
Hpy188I TCNGA 2 cut(s) 70, 696
Hpy188III TCNNGA 7 cut(s) 239, 345, 388, 577, 593, 683, 902
Hpy8I GTNNAC 5 cut(s) 167, 219, 509, 542, 631
HpyAV CCTTC 2 cut(s) 834, 1087
HpyCH4III ACNGT 3 cut(s) 574, 898, 1075
HpyCH4IV ACGT 1 cut(s) 544
HpyCH4V TGCA 8 cut(s) 107, 143, 186, 308, 524, 638, 814, 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 782, 820
HpyF3I CTNAG 1 cut(s) 492
HpySE526I ACGT 1 cut(s) 544
Hsp92II CATG 8 cut(s) 93, 147, 186, 190, 751, 776, 814, 1089
HspAI GCGC 1 cut(s) 821
Ksp22I TGATCA 1 cut(s) 861
Kzo9I GATC 6 cut(s) 70, 691, 861, 985, 1096, 1100
LmnI GCTCC 5 cut(s) 16, 209, 241, 760, 946
Lsp1109I GCAGC 2 cut(s) 830, 932
LweI GCATC 2 cut(s) 295, 676
MaeI CTAG 2 cut(s) 39, 942
MaeII ACGT 1 cut(s) 544
MalI GATC 6 cut(s) 72, 693, 863, 987, 1098, 1102
MboI GATC 6 cut(s) 70, 691, 861, 985, 1096, 1100
MboII GAAGA 9 cut(s) 259, 461, 467, 671, 683, 941, 944, 1055, 1058
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 1 cut(s) 765
MluCI AATT 7 cut(s) 76, 151, 365, 580, 850, 871, 935
MlyI GAGTC 1 cut(s) 167
Mph1103I ATGCAT 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 936
MseI TTAA 2 cut(s) 371, 1059
MspA1I CMGCKG 1 cut(s) 291
MspR9I CCNGG 4 cut(s) 119, 199, 830, 1013
MvaI CCWGG 4 cut(s) 119, 199, 830, 1013
MwoI GCNNNNNNNGC 2 cut(s) 782, 820
NcoI CCATGG 1 cut(s) 747
NdeII GATC 6 cut(s) 70, 691, 861, 985, 1096, 1100
NlaIII CATG 8 cut(s) 93, 147, 186, 190, 751, 776, 814, 1089
NlaIV GGNNCC 2 cut(s) 160, 287
NmeAIII GCCGAG 1 cut(s) 859
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 2 cut(s) 147, 814
PceI AGGCCT 1 cut(s) 37
PdmI GAANNNNTTC 1 cut(s) 936
PfeI GAWTC 2 cut(s) 341, 999
PflMI CCANNNNNTGG 1 cut(s) 1077
PkrI GCNGC 2 cut(s) 820, 922
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
Ppu21I YACGTR 1 cut(s) 545
PsiI TTATAA 1 cut(s) 846
Psp124BI GAGCTC 1 cut(s) 765
Psp6I CCWGG 4 cut(s) 117, 197, 828, 1011
PspFI CCCAGC 1 cut(s) 992
PspGI CCWGG 4 cut(s) 117, 197, 828, 1011
PspN4I GGNNCC 2 cut(s) 160, 287
PspPI GGNCC 3 cut(s) 195, 286, 1009
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 4 cut(s) 166, 543, 547, 911
RsaNI GTAC 4 cut(s) 165, 542, 546, 910
SacI GAGCTC 1 cut(s) 765
SaqAI TTAA 2 cut(s) 371, 1059
SatI GCNGC 2 cut(s) 819, 921
Sau3AI GATC 6 cut(s) 70, 691, 861, 985, 1096, 1100
Sau96I GGNCC 3 cut(s) 195, 286, 1009
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 4 cut(s) 119, 199, 830, 1013
SduI GDGCHC 1 cut(s) 765
SfaNI GCATC 2 cut(s) 295, 676
SfcI CTRYAG 1 cut(s) 1112
SinI GGWCC 1 cut(s) 286
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
SnaBI TACGTA 1 cut(s) 545
Sse9I AATT 7 cut(s) 76, 151, 365, 580, 850, 871, 935
SseBI AGGCCT 1 cut(s) 37
SsiI CCGC 2 cut(s) 283, 289
SspMI CTAG 2 cut(s) 39, 942
SstI GAGCTC 1 cut(s) 765
StuI AGGCCT 1 cut(s) 37
StyD4I CCNGG 4 cut(s) 117, 197, 828, 1011
StyI CCWWGG 3 cut(s) 14, 747, 941
TaaI ACNGT 3 cut(s) 574, 898, 1075
TaiI ACGT 1 cut(s) 547
TaqI TCGA 4 cut(s) 358, 624, 724, 1099
TasI AATT 7 cut(s) 76, 151, 365, 580, 850, 871, 935
TatI WGTACW 1 cut(s) 164
TfiI GAWTC 2 cut(s) 341, 999
Tru1I TTAA 2 cut(s) 371, 1059
Tru9I TTAA 2 cut(s) 371, 1059
TscAI CASTG 2 cut(s) 901, 1078
TseI GCWGC 2 cut(s) 818, 920
TspDTI ATGAA 8 cut(s) 354, 366, 462, 468, 501, 942, 1056, 1074
TspRI CASTG 2 cut(s) 901, 1078
Van91I CCANNNNNTGG 1 cut(s) 1077
VpaK11BI GGWCC 1 cut(s) 286
XapI RAATTY 4 cut(s) 365, 580, 871, 935
XceI RCATGY 2 cut(s) 147, 814
XmaJI CCTAGG 1 cut(s) 941
XmnI GAANNNNTTC 1 cut(s) 936
XspI CTAG 2 cut(s) 39, 942
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.