Prupe.8G223800_v2.0.a1

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001496
Physical Location & Seq
Reverse (-)
20197570 .. 20200954
3385 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G223800.1

Sequence Viewer

Length: 1617 bp
ATGGCGACTGCTTCAGTGGCGTTCAAATCTAGGGAAGACCATCGCAAACAAATCGAATTGGAAGAAGCGCGTAAAGCTGGGCTTGCACCAGCTGAACTGGATGAGGATGGAAAGGAGATTAATCCTCATATACCCCAATATATGTCATCTGCACCTTGGTATCTAAACTCTGAGAGGCCTAGCTTAAAACATCAAAGGAAATGGAAATCAGATCCTAATTATACAACATCATGGTATGACAGAGGTGCAAAGATACACCAGGCTGATAAATATAGGAAGGGTGCATGTGAGAACTGTGGTGCCATGACACACGACTCAAAGTCATGTATGGAAAGACCCAGGAGAGTGGGAGCAAAGTTCACAAACAAACACATCGCTCCTGATGAAAAGATAGAGACTTTTGAGCTTGACTATGATGGAAAACGGGACCGCTGGAATGGATATGATGCATCAACCTATGCCCGTGTCATTGAGAGATATGAATCAAGAGATGAAGCTCGAAGGAAATATGTAAAGACACAACAGCTCAAGAAATTAGAGGAGAAAAATAATAACCCAAATGGAGAGGACGGGGTTAGTGAGGAGGATGAGGATGATAATGAGGATGATTTGAGGGTGGATGAAGCCAAGGTTGATGAGAGCAAACAAATGGACTTTGCAAAGGTTGAGAAGCGTGTGCGTACAACGGGTGGTGGGAGCACAGGAACTGTCAGGAATTTGCGTATTCGTGAGGATACAGCAAAGTATCTTTTGAATCTCGATGTCGACTCTGCACACTATGACCCAAAAACCCGGTCTATGCGTGAGGACCCGCTTCCTGATGCGGATCCGAATGAGAAGTTCTATGGGGGTGATAATCGACATAGAAATAGTGGTCAAGCTATGGAGTTCAAGCAGCTCAACATCCATGCTTGGGAATCATTTGAGAAGGGACAAGATATTCACATGCAAGCAGCGCCGTCCCAAGCTGAGTTGCTTTATAAGAATTTTAAGGTGATCAAGGAGAATTTGAAGTCACAAACAAAGGACACCATTCTGGAGAAGTATGGCAATGCAGCTAATGAAGAAGAATTTCCTAGGGAGCTTCTACTGGGACAGAGTGAACGAGAAGTTGAATATGATCGTGCTGGGAGGATCATCAAGGGGCAAGAAACAACACTTCCCAGAAGCAAGTATGAAGAAGATGTATATGCCAACAACCACACCAGTGTATGGGGTTCATGGTGGAAGGATCATCAGTGGGGCTACAGATGCTGTAAGCAGTTGACTCGCAATAGCTATTGCACTGGCGCTGCTGGAATTGAGGCTGCTGAGGCTGCAGGTGAACTTATGAAGGCTAACATTGCTCGTAAAGAGGCCATTGGAGATATGCCTCCACCAGTGGAGGAGAAAAGACTTGCCACTTGGGGAACTGATATACCAGACGATTTGGTTTTAGATGAGAAGTTACTTGCTGATGCTCTTAAAAAGGAGGACGCAAGAAAGAGAGAAGTGAGAGATGAAAGAAAGCGCAAATACAATGTAAAATGGAATGATGAGGTTACTGCTGAGGATATGGAGGCTTATAGGATGAAGAAAGTTCATCATGATGATCCAATGAAGGACTTTCTTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Slu7

Protein Analysis

539

Amino Acids

62.38

Weight (kDa)

5.78

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AarI CACCTGC 1 cut(s) 1310
Acc36I ACCTGC 1 cut(s) 1310
AccB1I GGYRCC 1 cut(s) 299
AccB7I CCANNNNNTGG 1 cut(s) 1212
AccI GTMKAC 1 cut(s) 765
AccII CGCG 1 cut(s) 70
AciI CCGC 3 cut(s) 430, 812, 824
AclWI GGATC 6 cut(s) 206, 821, 834, 1142, 1239, 1586
AcsI RAATTY 4 cut(s) 715, 985, 1006, 1070
AfaI GTAC 1 cut(s) 682
AfiI CCNNNNNNNGG 2 cut(s) 913, 1212
AgsI TTSAA 5 cut(s) 25, 754, 892, 1012, 1115
AhdI GACNNNNNGTC 1 cut(s) 319
AjnI CCWGG 2 cut(s) 258, 338
AleI CACNNNNGTG 1 cut(s) 1206
Alw21I GWGCWC 1 cut(s) 701
Alw26I GTCTC 1 cut(s) 389
AlwI GGATC 6 cut(s) 206, 821, 834, 1142, 1239, 1586
AlwNI CAGNNNCTG 2 cut(s) 707, 1254
AoxI GGCC 2 cut(s) 176, 1356
ApeKI GCWGC 6 cut(s) 895, 953, 1055, 1292, 1307, 1316
ApoI RAATTY 4 cut(s) 715, 985, 1006, 1070
ArsI GACNNNNNNTTYG 2 cut(s) 779, 811
AseI ATTAAT 1 cut(s) 120
Asp700I GAANNNNTTC 1 cut(s) 1071
AspA2I CCTAGG 1 cut(s) 1076
AspLEI GCGC 4 cut(s) 70, 958, 1292, 1512
AspS9I GGNCC 2 cut(s) 427, 808
AsuC2I CCSGG 1 cut(s) 793
AsuHPI GGTGA 3 cut(s) 863, 1006, 1334
AvaII GGWCC 2 cut(s) 427, 808
AvrII CCTAGG 1 cut(s) 1076
BaeI ACNNNNGTAYC 2 cut(s) 143, 176
BamHI GGATCC 1 cut(s) 826
BanI GGYRCC 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 701
BbvCI CCTCAGC 2 cut(s) 1311, 1548
BbvI GCAGC 6 cut(s) 907, 965, 1067, 1279, 1294, 1303
BccI CCATC 3 cut(s) 48, 101, 410
BceAI ACGGC 1 cut(s) 943
BcgI CGANNNNNNTGC 4 cut(s) 34, 68, 1250, 1284
BciT130I CCWGG 2 cut(s) 260, 340
BciVI GTATCC 1 cut(s) 727
BclI TGATCA 1 cut(s) 996
BcnI CCSGG 1 cut(s) 793
BcoDI GTCTC 1 cut(s) 389
BfaI CTAG 3 cut(s) 30, 180, 1077
BfmI CTRYAG 2 cut(s) 1246, 1317
BfoI RGCGCY 2 cut(s) 959, 1293
BfuAI ACCTGC 1 cut(s) 1310
BfuI GTATCC 1 cut(s) 727
BisI GCNGC 6 cut(s) 896, 954, 1056, 1293, 1308, 1317
BlnI CCTAGG 1 cut(s) 1076
BlsI GCNGC 6 cut(s) 897, 955, 1057, 1294, 1309, 1318
Bme1390I CCNGG 3 cut(s) 260, 340, 793
Bme18I GGWCC 2 cut(s) 427, 808
BmeRI GACNNNNNGTC 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 427, 808
BmiI GGNNCC 4 cut(s) 301, 428, 810, 828
BmrFI CCNGG 3 cut(s) 260, 340, 793
BmrI ACTGGG 1 cut(s) 1100
BmsI GCATC 5 cut(s) 436, 458, 811, 1241, 1447
BmuI ACTGGG 1 cut(s) 1100
BpiI GAAGAC 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 1058
Bpu10I CCTNAGC 2 cut(s) 1311, 1548
BpuEI CTTGAG 1 cut(s) 512
BpuMI CCSGG 1 cut(s) 793
BsaBI GATNNNNATC 2 cut(s) 481, 825
BsaJI CCNNGG 4 cut(s) 155, 338, 627, 1076
Bsc4I CCNNNNNNNGG 2 cut(s) 913, 1212
Bse1I ACTGG 5 cut(s) 102, 1095, 1206, 1291, 1379
Bse3DI GCAATG 2 cut(s) 1057, 1341
Bse8I GATNNNNATC 2 cut(s) 481, 825
BseBI CCWGG 2 cut(s) 260, 340
BseDI CCNNGG 4 cut(s) 155, 338, 627, 1076
BseGI GGATG 8 cut(s) 106, 112, 592, 598, 610, 625, 903, 1575
BseJI GATNNNNATC 2 cut(s) 481, 825
BseLI CCNNNNNNNGG 2 cut(s) 913, 1212
BseMI GCAATG 2 cut(s) 1057, 1341
BseMII CTCAG 4 cut(s) 162, 960, 1302, 1539
BseNI ACTGG 5 cut(s) 102, 1095, 1206, 1291, 1379
BseRI GAGGAG 3 cut(s) 554, 596, 1400
BseXI GCAGC 6 cut(s) 907, 965, 1067, 1279, 1294, 1303
BseYI CCCAGC 2 cut(s) 77, 1127
BsgI GTGCAG 2 cut(s) 135, 756
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 2 cut(s) 178, 1358
BshNI GGYRCC 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 701
BsiSI CCGG 1 cut(s) 793
BslFI GGGAC 4 cut(s) 440, 945, 946, 1107
BslI CCNNNNNNNGG 2 cut(s) 913, 1212
BsmAI GTCTC 1 cut(s) 389
BsmFI GGGAC 4 cut(s) 440, 945, 946, 1107
BsnI GGCC 2 cut(s) 178, 1358
Bsp1286I GDGCHC 1 cut(s) 701
Bsp143I GATC 7 cut(s) 211, 826, 996, 1120, 1134, 1231, 1591
BspACI CCGC 3 cut(s) 430, 812, 824
BspANI GGCC 2 cut(s) 178, 1358
BspCNI CTCAG 4 cut(s) 163, 961, 1303, 1540
BspFNI CGCG 1 cut(s) 70
BspHI TCATGA 1 cut(s) 1585
BspLI GGNNCC 4 cut(s) 301, 428, 810, 828
BspMAI CTGCAG 1 cut(s) 1321
BspMI ACCTGC 1 cut(s) 1310
BspPI GGATC 6 cut(s) 206, 821, 834, 1142, 1239, 1586
BspT107I GGYRCC 1 cut(s) 299
BsrDI GCAATG 2 cut(s) 1057, 1341
BsrI ACTGG 5 cut(s) 102, 1095, 1206, 1291, 1379
BssECI CCNNGG 4 cut(s) 155, 338, 627, 1076
BssMI GATC 7 cut(s) 211, 826, 996, 1120, 1134, 1231, 1591
BssT1I CCWWGG 3 cut(s) 155, 627, 1076
Bst2UI CCWGG 2 cut(s) 260, 340
Bst4CI ACNGT 2 cut(s) 296, 709
BstC8I GCNNGC 2 cut(s) 84, 951
BstDEI CTNAG 4 cut(s) 171, 969, 1311, 1548
BstF5I GGATG 8 cut(s) 106, 112, 592, 598, 610, 625, 903, 1575
BstFNI CGCG 1 cut(s) 70
BstH2I RGCGCY 2 cut(s) 959, 1293
BstHHI GCGC 4 cut(s) 70, 958, 1292, 1512
BstKTI GATC 7 cut(s) 214, 829, 999, 1123, 1137, 1234, 1594
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 7 cut(s) 211, 826, 996, 1120, 1134, 1231, 1591
BstMWI GCNNNNNNNGC 8 cut(s) 17, 74, 83, 955, 1251, 1313, 1316, 1343
BstNI CCWGG 2 cut(s) 260, 340
BstNSI RCATGY 2 cut(s) 288, 949
BstSCI CCNGG 3 cut(s) 258, 338, 791
BstSFI CTRYAG 2 cut(s) 1246, 1317
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 6 cut(s) 907, 965, 1067, 1279, 1294, 1303
BstV2I GAAGAC 1 cut(s) 42
BstX2I RGATCY 2 cut(s) 211, 826
BstXI CCANNNNNNTGG 1 cut(s) 346
BstYI RGATCY 2 cut(s) 211, 826
BsuI GTATCC 1 cut(s) 727
BsuRI GGCC 2 cut(s) 178, 1358
BtgZI GCGATG 2 cut(s) 26, 358
BtsCI GGATG 8 cut(s) 106, 112, 592, 598, 610, 625, 903, 1575
BtsIMutI CAGTG 5 cut(s) 21, 1213, 1244, 1284, 1386
BveI ACCTGC 1 cut(s) 1310
Cac8I GCNNGC 2 cut(s) 84, 951
CaiI CAGNNNCTG 2 cut(s) 707, 1254
CciI TCATGA 1 cut(s) 1585
CfoI GCGC 4 cut(s) 70, 958, 1292, 1512
Cfr13I GGNCC 2 cut(s) 427, 808
CseI GACGC 1 cut(s) 1484
Csp6I GTAC 1 cut(s) 681
CviAII CATG 8 cut(s) 231, 285, 304, 324, 908, 946, 1221, 1586
CviQI GTAC 1 cut(s) 681
DdeI CTNAG 4 cut(s) 171, 969, 1311, 1548
DpnI GATC 7 cut(s) 213, 828, 998, 1122, 1136, 1233, 1593
DpnII GATC 7 cut(s) 211, 826, 996, 1120, 1134, 1231, 1591
DriI GACNNNNNGTC 1 cut(s) 319
Eam1105I GACNNNNNGTC 1 cut(s) 319
Eco130I CCWWGG 3 cut(s) 155, 627, 1076
Eco147I AGGCCT 1 cut(s) 178
Eco47I GGWCC 2 cut(s) 427, 808
EcoO109I RGGNCCY 1 cut(s) 808
EcoRII CCWGG 2 cut(s) 258, 338
EcoT14I CCWWGG 3 cut(s) 155, 627, 1076
EcoT22I ATGCAT 1 cut(s) 451
ErhI CCWWGG 3 cut(s) 155, 627, 1076
FaeI CATG 8 cut(s) 234, 288, 307, 327, 911, 949, 1224, 1589
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FaqI GGGAC 4 cut(s) 440, 945, 946, 1107
FatI CATG 8 cut(s) 230, 284, 303, 323, 907, 945, 1220, 1585
FauI CCCGC 1 cut(s) 819
FbaI TGATCA 1 cut(s) 996
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 6 cut(s) 896, 954, 1056, 1293, 1308, 1317
FokI GGATG 8 cut(s) 113, 119, 599, 605, 617, 632, 890, 1582
Fsp4HI GCNGC 6 cut(s) 896, 954, 1056, 1293, 1308, 1317
FspBI CTAG 3 cut(s) 30, 180, 1077
GlaI GCGC 4 cut(s) 69, 957, 1291, 1511
GluI GCNGC 6 cut(s) 896, 954, 1056, 1293, 1308, 1317
GsaI CCCAGC 2 cut(s) 81, 1131
GsuI CTGGAG 1 cut(s) 1058
HaeII RGCGCY 2 cut(s) 959, 1293
HaeIII GGCC 2 cut(s) 178, 1358
HapII CCGG 1 cut(s) 793
HgaI GACGC 1 cut(s) 1484
HhaI GCGC 4 cut(s) 70, 958, 1292, 1512
Hin1II CATG 8 cut(s) 234, 288, 307, 327, 911, 949, 1224, 1589
Hin6I GCGC 4 cut(s) 68, 956, 1290, 1510
HinP1I GCGC 4 cut(s) 68, 956, 1290, 1510
HincII GTYRAC 2 cut(s) 766, 1266
HindII GTYRAC 2 cut(s) 766, 1266
HinfI GANTC 6 cut(s) 314, 482, 754, 767, 917, 1267
HpaII CCGG 1 cut(s) 793
HphI GGTGA 3 cut(s) 863, 1006, 1334
Hpy166II GTNNAC 5 cut(s) 360, 766, 1103, 1266, 1325
Hpy188I TCNGA 3 cut(s) 172, 211, 831
Hpy188III TCNNGA 9 cut(s) 380, 486, 529, 712, 728, 758, 818, 1037, 1586
Hpy8I GTNNAC 5 cut(s) 360, 766, 1103, 1266, 1325
HpyAV CCTTC 6 cut(s) 271, 495, 922, 1222, 1327, 1594
HpyCH4III ACNGT 2 cut(s) 296, 709
HpyF10VI GCNNNNNNNGC 8 cut(s) 17, 74, 83, 955, 1251, 1313, 1316, 1343
HpyF3I CTNAG 4 cut(s) 171, 969, 1311, 1548
Hsp92II CATG 8 cut(s) 234, 288, 307, 327, 911, 949, 1224, 1589
HspAI GCGC 4 cut(s) 68, 956, 1290, 1510
Ksp22I TGATCA 1 cut(s) 996
Kzo9I GATC 7 cut(s) 211, 826, 996, 1120, 1134, 1231, 1591
LmnI GCTCC 4 cut(s) 350, 382, 696, 1081
Lsp1109I GCAGC 6 cut(s) 907, 965, 1067, 1279, 1294, 1303
LweI GCATC 5 cut(s) 436, 458, 811, 1241, 1447
MaeI CTAG 3 cut(s) 30, 180, 1077
MaeIII GTNAC 3 cut(s) 1014, 1446, 1540
MalI GATC 7 cut(s) 213, 828, 998, 1122, 1136, 1233, 1593
MboI GATC 7 cut(s) 211, 826, 996, 1120, 1134, 1231, 1591
MboII GAAGA 7 cut(s) 47, 74, 1076, 1079, 1190, 1193, 1585
MflI RGATCY 2 cut(s) 211, 826
MhlI GDGCHC 1 cut(s) 701
MluCI AATT 8 cut(s) 56, 217, 533, 715, 985, 1006, 1070, 1299
MlyI GAGTC 3 cut(s) 308, 761, 1261
Mph1103I ATGCAT 1 cut(s) 451
MroXI GAANNNNTTC 1 cut(s) 1071
MseI TTAA 5 cut(s) 120, 185, 990, 1464, 1611
MslI CAYNNNNRTG 2 cut(s) 1206, 1587
MspA1I CMGCKG 2 cut(s) 92, 432
MspI CCGG 1 cut(s) 793
MspR9I CCNGG 3 cut(s) 260, 340, 793
MvaI CCWGG 2 cut(s) 260, 340
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 8 cut(s) 17, 74, 83, 955, 1251, 1313, 1316, 1343
NciI CCSGG 1 cut(s) 793
NdeII GATC 7 cut(s) 211, 826, 996, 1120, 1134, 1231, 1591
NlaIII CATG 8 cut(s) 234, 288, 307, 327, 911, 949, 1224, 1589
NlaIV GGNNCC 4 cut(s) 301, 428, 810, 828
NmuCI GTSAC 1 cut(s) 1014
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 2 cut(s) 288, 949
OliI CACNNNNGTG 1 cut(s) 1206
PagI TCATGA 1 cut(s) 1585
PaqCI CACCTGC 1 cut(s) 1310
PceI AGGCCT 1 cut(s) 178
PdmI GAANNNNTTC 1 cut(s) 1071
PfeI GAWTC 3 cut(s) 482, 754, 917
PflMI CCANNNNNTGG 1 cut(s) 1212
PkrI GCNGC 6 cut(s) 897, 955, 1057, 1294, 1309, 1318
PleI GAGTC 3 cut(s) 308, 761, 1261
PpsI GAGTC 3 cut(s) 308, 761, 1261
PpuMI RGGWCCY 1 cut(s) 808
PshBI ATTAAT 1 cut(s) 120
PsiI TTATAA 1 cut(s) 981
Psp5II RGGWCCY 1 cut(s) 808
Psp6I CCWGG 2 cut(s) 258, 338
PspFI CCCAGC 2 cut(s) 77, 1127
PspGI CCWGG 2 cut(s) 258, 338
PspN4I GGNNCC 4 cut(s) 301, 428, 810, 828
PspPI GGNCC 2 cut(s) 427, 808
PspPPI RGGWCCY 1 cut(s) 808
PstI CTGCAG 1 cut(s) 1321
PstNI CAGNNNCTG 2 cut(s) 707, 1254
PsuI RGATCY 2 cut(s) 211, 826
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 1 cut(s) 682
RsaNI GTAC 1 cut(s) 681
RseI CAYNNNNRTG 2 cut(s) 1206, 1587
SalI GTCGAC 1 cut(s) 764
SaqAI TTAA 5 cut(s) 120, 185, 990, 1464, 1611
SatI GCNGC 6 cut(s) 896, 954, 1056, 1293, 1308, 1317
Sau3AI GATC 7 cut(s) 211, 826, 996, 1120, 1134, 1231, 1591
Sau96I GGNCC 2 cut(s) 427, 808
SchI GAGTC 3 cut(s) 308, 761, 1261
ScrFI CCNGG 3 cut(s) 260, 340, 793
SduI GDGCHC 1 cut(s) 701
SfaNI GCATC 5 cut(s) 436, 458, 811, 1241, 1447
SfcI CTRYAG 2 cut(s) 1246, 1317
SinI GGWCC 2 cut(s) 427, 808
SmiMI CAYNNNNRTG 2 cut(s) 1206, 1587
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
Sse9I AATT 8 cut(s) 56, 217, 533, 715, 985, 1006, 1070, 1299
SseBI AGGCCT 1 cut(s) 178
SsiI CCGC 3 cut(s) 430, 812, 824
SspMI CTAG 3 cut(s) 30, 180, 1077
StuI AGGCCT 1 cut(s) 178
StyD4I CCNGG 3 cut(s) 258, 338, 791
StyI CCWWGG 3 cut(s) 155, 627, 1076
TaaI ACNGT 2 cut(s) 296, 709
TaqI TCGA 5 cut(s) 54, 499, 759, 765, 859
TasI AATT 8 cut(s) 56, 217, 533, 715, 985, 1006, 1070, 1299
TfiI GAWTC 3 cut(s) 482, 754, 917
Tru1I TTAA 5 cut(s) 120, 185, 990, 1464, 1611
Tru9I TTAA 5 cut(s) 120, 185, 990, 1464, 1611
TscAI CASTG 5 cut(s) 21, 1213, 1244, 1291, 1386
TseFI GTSAC 1 cut(s) 1014
TseI GCWGC 6 cut(s) 895, 953, 1055, 1292, 1307, 1316
Tsp45I GTSAC 1 cut(s) 1014
TspRI CASTG 5 cut(s) 21, 1213, 1244, 1291, 1386
Van91I CCANNNNNTGG 1 cut(s) 1212
VpaK11BI GGWCC 2 cut(s) 427, 808
VspI ATTAAT 1 cut(s) 120
XapI RAATTY 4 cut(s) 715, 985, 1006, 1070
XceI RCATGY 2 cut(s) 288, 949
XmaJI CCTAGG 1 cut(s) 1076
XmiI GTMKAC 1 cut(s) 765
XmnI GAANNNNTTC 1 cut(s) 1071
XspI CTAG 3 cut(s) 30, 180, 1077
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.