Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001496

Physical Location & Seq

Genomic Coordinates Reverse (-)

32448362 .. 32452056

Gene Structure 3695 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1197300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGGCGACCGCTTCAGGGTCATTCAAATCTAGGGAAGACCATCGTAAGCAAATAGAATTGGAAGAAGCGCGTAAAGCTGGGCTTGCACCAGCTGAACTGGACGAGGATGGAAAAGAGATTAATCCTCATATTCCCCAATATATGTCATCTGCTCCTTGGTATCTCAAACACGAGAGGCCTAGCTTGAAACATCAAAGGAAATGGAAATCAGATCCAAATTATACAACATCATGGTATGACAGAGGTGCAAAGATATTCCAGGCTGATAAATATAGAAAGGGTGCATGTGAGAACTGTGGTGCCAGTACACACGACTCCAAGTCATGCATGGATAGGCCCAGGAAAGTGGGAGCAAAGTGGACAAACACACACATTGCTCCTGATGAGAAGATAGAGACTTTTGAGCTTGACTATGATGGAAAGCGGGACCGCTGGAATGGATATGATGCAACGAGCTATGCTCTTGTAGTAGAGAGATATGAATCAAGAGATGAAGCTCGAAAGAAATTCTTAAAGGAACAGCAACTCAAGAAATTAGAGGAGAAAAATAATAATCCAAATGGCGAGGACAAGGTTAGTGATGTGGATGAAGATGAAGACGAGGATGAGGATGATTTGAGGGTAGATGAAGCCAAGGTTGATGAGAGTAAACAAATGGACTTTGCAAAGGTTGAGAAGCGTGTACGTACAACTGGTGGTGGAAGCACAGGAACTGTCAGGAATTTGCGTATTCGTGAGGATACAGCGAAATATCTTTTGAACCTCGATGTCAACTCTGCACATTATGACCCCAAAACCCGATCTATGCGGGAAGACCCTCTTCCAGATGCTGATCCGAATGAGAAGTTTTATGGGGGTGATAATCGATATAGAAATAGTGGTCAAGCCATGGAGTTCAAGGAGCTCAACATCCATGCTTGGGAAGCATTTGAGAAGGGACAAGATATCCACATGCAAGCAGCGCCTTCCCAGGCGGAGTTGCTTTATAAGAATTATCAGGTGATCAAGGAGAATTTGAAGTCAAAAACAAAAGACACTGTTCTGGAGAAGTACGGCAATGCAGCTAATGAAGAAGAATTTCCTAGGGAGCTTCTACTGGGACAAAGTGAAAGGGAAGTTGAATATGATCGTGCTGGGAGAATCATCAAGGGCCAGGATATAGCACTTCCCAGAAGCAAGTATGAAGAAGATGTCTATGTCAATAACCACACCACTGTATGGGGTTCATGGTGGAAGGATCATCAATGGGGCTACAGGTGTTGTAAGCAGGTTACTCGCAATAGCTATTGCACGGGCGCTGCTGGAATTGAGGCTGCTGAGGCTGCAACTGATCTTATGAAGGCTAACATTGCGCGTAAAGAGGCCAATGGAGATGTGCCTGCACCAGTGGAGGAGAAAAGACCTGCTACTTGGGGAACTGATATCCCAGATGATTTGGTTTTAGATGAGAAATTACTTGCTGAAGCTCTTAAGAAGGAGGATCAGAGAAAGAGAGAAGAGAGAGACGAGAGAAAGCGCAAATACAATGTTAAATGGAACGATGATGTTACTGCTGAGGATATGGAAGCCTATAGGATGAAGAAGGTCCACCATGATGATCCAATGAAGGACTTTCTGAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

Slu7

Protein Analysis

541

Amino Acids

62.63

Weight (kDa)

5.51

Isoelectric Point (pI)

38.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Slu7	PF11708	136 - 409	3.6e-93	Pre-mRNA splicing Prp18-interacting factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001496)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65660 AT3G45950 AT4G37120
fragaria_vesca	FvH4_2g21480 FvH4_4g03640 FvH4_5g13320
malus_domestica	MD05G1197300.v1.1 MD10G1184000.v1.1 MD16G1219100.v1.1
prunus_persica	Prupe.1G039500_v2.0.a1 Prupe.1G039500_v2.0.a1 Prupe.1G039500_v2.0.a1 Prupe.1G039500_v2.0.a1 Prupe.1G039500_v2.0.a1 Prupe.1G039500_v2.0.a1 Prupe.1G039500_v2.0.a1 Prupe.8G223800_v2.0.a1
pyrus_communis	pycom05g18440 pycom10g15930 pycom16g18260
rosa_chinensis	RchiOBHm_Chr4g0393021 RchiOBHm_Chr6g0288131 RchiOBHm_Chr7g0183821
rosa_laevigata	RLG00000005020 RLG00000009774
rosa_multiflora	Rmu_sc0003610.1_g000043 Rmu_sc0010766.1_g000006 Rmu_sc0034700.1_g000001
rosa_roxburghii	Rroxscaffold_3G00270180 Rroxscaffold_5G00338120 Rroxscaffold_7G00180470
rosa_rugosa	Rorug04G0002700 Rorug04G0002800 Rorug06G0194300 Rorug06G0460700 Rorug06G0460800
rosa_samantha	Rh4AG045400 Rh4BG041400 Rh4CG049000 Rh4DG043300 Rh6AG304700 Rh6BG310700 Rh6CG318800 Rh6DG303700 Rh7AG061400 Rh7BG061500 Rh7CG063100 Rh7DG061500
rosa_wichuraiana	Rw4G003580 Rw6G026360 Rw7G005100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	987
Acc36I	ACCTGC	2 cut(s)	1258, 1411
AccB1I	GGYRCC	1 cut(s)	299
AccB7I	CCANNNNNTGG	1 cut(s)	1218
AccII	CGCG	2 cut(s)	70, 1354
AciI	CCGC	5 cut(s)	9, 424, 430, 808, 974
AclWI	GGATC	5 cut(s)	206, 827, 1245, 1488, 1592
AcsI	RAATTY	4 cut(s)	506, 721, 1012, 1076
AcuI	CTGAAG	1 cut(s)	1482
AfaI	GTAC	4 cut(s)	307, 684, 688, 1052
AfiI	CCNNNNNNNGG	3 cut(s)	15, 919, 1218
AflII	CTTAAG	1 cut(s)	1469
AgsI	TTSAA	6 cut(s)	25, 187, 760, 898, 1018, 1121
AhdI	GACNNNNNGTC	1 cut(s)	319
AjnI	CCWGG	4 cut(s)	258, 338, 969, 1152
Alw21I	GWGCWC	1 cut(s)	906
Alw26I	GTCTC	2 cut(s)	389, 1497
AlwI	GGATC	5 cut(s)	206, 827, 1245, 1488, 1592
AlwNI	CAGNNNCTG	2 cut(s)	713, 830
AoxI	GGCC	4 cut(s)	176, 335, 1150, 1362
ApeKI	GCWGC	5 cut(s)	959, 1061, 1298, 1313, 1322
ApoI	RAATTY	4 cut(s)	506, 721, 1012, 1076
AseI	ATTAAT	1 cut(s)	120
Asp700I	GAANNNNTTC	1 cut(s)	1077
AspA2I	CCTAGG	1 cut(s)	1082
AspLEI	GCGC	5 cut(s)	70, 964, 1298, 1354, 1518
AspS9I	GGNCC	4 cut(s)	336, 427, 1150, 1585
AsuHPI	GGTGA	2 cut(s)	869, 1012
AvaII	GGWCC	2 cut(s)	427, 1585
AvrII	CCTAGG	1 cut(s)	1082
BanI	GGYRCC	1 cut(s)	299
BanII	GRGCYC	1 cut(s)	906
BauI	CACGAG	1 cut(s)	170
BbsI	GAAGAC	3 cut(s)	42, 603, 819
Bbv12I	GWGCWC	1 cut(s)	906
BbvCI	CCTCAGC	2 cut(s)	1317, 1554
BbvI	GCAGC	5 cut(s)	971, 1073, 1285, 1300, 1309
BccI	CCATC	3 cut(s)	48, 101, 410
BceAI	ACGGC	1 cut(s)	1069
BcgI	CGANNNNNNTGC	2 cut(s)	1256, 1290
BciT130I	CCWGG	4 cut(s)	260, 340, 971, 1154
BciVI	GTATCC	1 cut(s)	733
BclI	TGATCA	1 cut(s)	1002
BcoDI	GTCTC	2 cut(s)	389, 1497
BfaI	CTAG	3 cut(s)	30, 180, 1083
BfmI	CTRYAG	2 cut(s)	1252, 1570
BfoI	RGCGCY	2 cut(s)	965, 1299
BfrI	CTTAAG	1 cut(s)	1469
BfuAI	ACCTGC	2 cut(s)	1258, 1411
BfuI	GTATCC	1 cut(s)	733
BisI	GCNGC	5 cut(s)	960, 1062, 1299, 1314, 1323
BlnI	CCTAGG	1 cut(s)	1082
BlsI	GCNGC	5 cut(s)	961, 1063, 1300, 1315, 1324
Bme1390I	CCNGG	4 cut(s)	260, 340, 971, 1154
Bme18I	GGWCC	2 cut(s)	427, 1585
BmeRI	GACNNNNNGTC	1 cut(s)	319
BmgT120I	GGNCC	4 cut(s)	336, 427, 1150, 1585
BmiI	GGNNCC	2 cut(s)	301, 428
BmrFI	CCNGG	4 cut(s)	260, 340, 971, 1154
BmrI	ACTGGG	1 cut(s)	1106
BmsI	GCATC	2 cut(s)	436, 817
BmuI	ACTGGG	1 cut(s)	1106
BpiI	GAAGAC	3 cut(s)	42, 603, 819
BplI	GAGNNNNNCTC	2 cut(s)	445, 477
BpmI	CTGGAG	1 cut(s)	1064
Bpu10I	CCTNAGC	2 cut(s)	1317, 1554
BpuEI	CTTGAG	1 cut(s)	512
Bsa29I	ATCGAT	1 cut(s)	865
BsaAI	YACGTR	1 cut(s)	686
BsaBI	GATNNNNATC	2 cut(s)	481, 831
BsaJI	CCNNGG	6 cut(s)	155, 338, 633, 888, 969, 1082
Bsc4I	CCNNNNNNNGG	3 cut(s)	15, 919, 1218
Bse1I	ACTGG	5 cut(s)	102, 303, 697, 1101, 1385
Bse3DI	GCAATG	3 cut(s)	372, 1063, 1347
Bse8I	GATNNNNATC	2 cut(s)	481, 831
BseBI	CCWGG	4 cut(s)	260, 340, 971, 1154
BseCI	ATCGAT	1 cut(s)	865
BseDI	CCNNGG	6 cut(s)	155, 338, 633, 888, 969, 1082
BseGI	GGATG	6 cut(s)	112, 592, 610, 616, 909, 1581
BseJI	GATNNNNATC	2 cut(s)	481, 831
BseLI	CCNNNNNNNGG	3 cut(s)	15, 919, 1218
BseMI	GCAATG	3 cut(s)	372, 1063, 1347
BseMII	CTCAG	2 cut(s)	1308, 1545
BseNI	ACTGG	5 cut(s)	102, 303, 697, 1101, 1385
BseRI	GAGGAG	2 cut(s)	554, 1406
BseXI	GCAGC	5 cut(s)	971, 1073, 1285, 1300, 1309
BseYI	CCCAGC	2 cut(s)	77, 1133
BsgI	GTGCAG	2 cut(s)	762, 1365
Bsh1236I	CGCG	2 cut(s)	70, 1354
Bsh1285I	CGRYCG	1 cut(s)	9
BshFI	GGCC	4 cut(s)	178, 337, 1152, 1364
BshNI	GGYRCC	1 cut(s)	299
BshVI	ATCGAT	1 cut(s)	865
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	1 cut(s)	906
BslFI	GGGAC	3 cut(s)	440, 951, 1113
BslI	CCNNNNNNNGG	3 cut(s)	15, 919, 1218
BsmAI	GTCTC	2 cut(s)	389, 1497
BsmBI	CGTCTC	1 cut(s)	1497
BsmFI	GGGAC	3 cut(s)	440, 951, 1113
BsnI	GGCC	4 cut(s)	178, 337, 1152, 1364
Bsp1286I	GDGCHC	1 cut(s)	906
Bsp143I	GATC	9 cut(s)	211, 800, 832, 1002, 1126, 1237, 1330, 1480, 1597
Bsp19I	CCATGG	1 cut(s)	888
BspACI	CCGC	5 cut(s)	9, 424, 430, 808, 974
BspANI	GGCC	4 cut(s)	178, 337, 1152, 1364
BspCNI	CTCAG	2 cut(s)	1309, 1546
BspDI	ATCGAT	1 cut(s)	865
BspFNI	CGCG	2 cut(s)	70, 1354
BspLI	GGNNCC	2 cut(s)	301, 428
BspMI	ACCTGC	2 cut(s)	1258, 1411
BspPI	GGATC	5 cut(s)	206, 827, 1245, 1488, 1592
BspT107I	GGYRCC	1 cut(s)	299
BspTI	CTTAAG	1 cut(s)	1469
BsrDI	GCAATG	3 cut(s)	372, 1063, 1347
BsrI	ACTGG	5 cut(s)	102, 303, 697, 1101, 1385
BssECI	CCNNGG	6 cut(s)	155, 338, 633, 888, 969, 1082
BssMI	GATC	9 cut(s)	211, 800, 832, 1002, 1126, 1237, 1330, 1480, 1597
BssSI	CACGAG	1 cut(s)	170
BssT1I	CCWWGG	4 cut(s)	155, 633, 888, 1082
Bst2BI	CACGAG	1 cut(s)	170
Bst2UI	CCWGG	4 cut(s)	260, 340, 971, 1154
Bst4CI	ACNGT	4 cut(s)	296, 715, 1039, 1216
Bst6I	CTCTTC	2 cut(s)	825, 1491
BstAFI	CTTAAG	1 cut(s)	1469
BstBAI	YACGTR	1 cut(s)	686
BstC8I	GCNNGC	3 cut(s)	84, 957, 1380
BstDEI	CTNAG	2 cut(s)	1317, 1554
BstDSI	CCRYGG	1 cut(s)	888
BstF5I	GGATG	6 cut(s)	112, 592, 610, 616, 909, 1581
BstFNI	CGCG	2 cut(s)	70, 1354
BstH2I	RGCGCY	2 cut(s)	965, 1299
BstHHI	GCGC	5 cut(s)	70, 964, 1298, 1354, 1518
BstKTI	GATC	9 cut(s)	214, 803, 835, 1005, 1129, 1240, 1333, 1483, 1600
BstMAI	GTCTC	2 cut(s)	389, 1497
BstMBI	GATC	9 cut(s)	211, 800, 832, 1002, 1126, 1237, 1330, 1480, 1597
BstMCI	CGRYCG	1 cut(s)	9
BstMWI	GCNNNNNNNGC	7 cut(s)	74, 83, 923, 961, 1319, 1322, 1349
BstNI	CCWGG	4 cut(s)	260, 340, 971, 1154
BstNSI	RCATGY	2 cut(s)	288, 955
BstSCI	CCNGG	4 cut(s)	258, 338, 969, 1152
BstSFI	CTRYAG	2 cut(s)	1252, 1570
BstSNI	TACGTA	1 cut(s)	686
BstUI	CGCG	2 cut(s)	70, 1354
BstV1I	GCAGC	5 cut(s)	971, 1073, 1285, 1300, 1309
BstV2I	GAAGAC	3 cut(s)	42, 603, 819
BstX2I	RGATCY	1 cut(s)	211
BstXI	CCANNNNNNTGG	1 cut(s)	346
BstYI	RGATCY	1 cut(s)	211
Bsu15I	ATCGAT	1 cut(s)	865
BsuI	GTATCC	1 cut(s)	733
BsuRI	GGCC	4 cut(s)	178, 337, 1152, 1364
BsuTUI	ATCGAT	1 cut(s)	865
BtgI	CCRYGG	1 cut(s)	888
BtsCI	GGATG	6 cut(s)	112, 592, 610, 616, 909, 1581
BtsIMutI	CAGTG	3 cut(s)	1035, 1212, 1392
BveI	ACCTGC	2 cut(s)	1258, 1411
Cac8I	GCNNGC	3 cut(s)	84, 957, 1380
CaiI	CAGNNNCTG	2 cut(s)	713, 830
CfoI	GCGC	5 cut(s)	70, 964, 1298, 1354, 1518
Cfr13I	GGNCC	4 cut(s)	336, 427, 1150, 1585
ClaI	ATCGAT	1 cut(s)	865
Csp6I	GTAC	4 cut(s)	306, 683, 687, 1051
CviAII	CATG	9 cut(s)	231, 285, 324, 328, 889, 914, 952, 1227, 1592
CviQI	GTAC	4 cut(s)	306, 683, 687, 1051
DdeI	CTNAG	2 cut(s)	1317, 1554
DpnI	GATC	9 cut(s)	213, 802, 834, 1004, 1128, 1239, 1332, 1482, 1599
DpnII	GATC	9 cut(s)	211, 800, 832, 1002, 1126, 1237, 1330, 1480, 1597
DriI	GACNNNNNGTC	1 cut(s)	319
Eam1104I	CTCTTC	2 cut(s)	825, 1491
Eam1105I	GACNNNNNGTC	1 cut(s)	319
EarI	CTCTTC	2 cut(s)	825, 1491
EciI	GGCGGA	1 cut(s)	989
Ecl136II	GAGCTC	1 cut(s)	904
Eco105I	TACGTA	1 cut(s)	686
Eco130I	CCWWGG	4 cut(s)	155, 633, 888, 1082
Eco147I	AGGCCT	1 cut(s)	178
Eco24I	GRGCYC	1 cut(s)	906
Eco32I	GATATC	2 cut(s)	946, 1423
Eco47I	GGWCC	2 cut(s)	427, 1585
Eco53kI	GAGCTC	1 cut(s)	904
Eco57I	CTGAAG	1 cut(s)	1482
EcoICRI	GAGCTC	1 cut(s)	904
EcoRII	CCWGG	4 cut(s)	258, 338, 969, 1152
EcoRV	GATATC	2 cut(s)	946, 1423
EcoT14I	CCWWGG	4 cut(s)	155, 633, 888, 1082
EcoT22I	ATGCAT	1 cut(s)	329
EcoT38I	GRGCYC	1 cut(s)	906
ErhI	CCWWGG	4 cut(s)	155, 633, 888, 1082
Esp3I	CGTCTC	1 cut(s)	1497
FaeI	CATG	9 cut(s)	234, 288, 327, 331, 892, 917, 955, 1230, 1595
FalI	AAGNNNNNCTT	6 cut(s)	66, 98, 494, 526, 804, 836
FaqI	GGGAC	3 cut(s)	440, 951, 1113
FatI	CATG	9 cut(s)	230, 284, 323, 327, 888, 913, 951, 1226, 1591
FauI	CCCGC	2 cut(s)	417, 801
FbaI	TGATCA	1 cut(s)	1002
Fnu4HI	GCNGC	5 cut(s)	960, 1062, 1299, 1314, 1323
FokI	GGATG	6 cut(s)	119, 599, 617, 623, 896, 1588
FriOI	GRGCYC	1 cut(s)	906
Fsp4HI	GCNGC	5 cut(s)	960, 1062, 1299, 1314, 1323
FspBI	CTAG	3 cut(s)	30, 180, 1083
GlaI	GCGC	5 cut(s)	69, 963, 1297, 1353, 1517
GluI	GCNGC	5 cut(s)	960, 1062, 1299, 1314, 1323
GsaI	CCCAGC	2 cut(s)	81, 1137
GsuI	CTGGAG	1 cut(s)	1064
HaeII	RGCGCY	2 cut(s)	965, 1299
HaeIII	GGCC	4 cut(s)	178, 337, 1152, 1364
HhaI	GCGC	5 cut(s)	70, 964, 1298, 1354, 1518
Hin1II	CATG	9 cut(s)	234, 288, 327, 331, 892, 917, 955, 1230, 1595
Hin6I	GCGC	5 cut(s)	68, 962, 1296, 1352, 1516
HinP1I	GCGC	5 cut(s)	68, 962, 1296, 1352, 1516
HincII	GTYRAC	1 cut(s)	772
HindII	GTYRAC	1 cut(s)	772
HinfI	GANTC	3 cut(s)	314, 482, 1140
HphI	GGTGA	2 cut(s)	869, 1012
Hpy166II	GTNNAC	6 cut(s)	308, 360, 650, 683, 772, 1588
Hpy188I	TCNGA	4 cut(s)	211, 837, 1485, 1617
Hpy188III	TCNNGA	7 cut(s)	380, 486, 529, 718, 734, 824, 1043
Hpy8I	GTNNAC	6 cut(s)	308, 360, 650, 683, 772, 1588
HpyAV	CCTTC	7 cut(s)	928, 975, 1228, 1333, 1468, 1576, 1600
HpyCH4III	ACNGT	4 cut(s)	296, 715, 1039, 1216
HpyCH4IV	ACGT	1 cut(s)	685
HpyF10VI	GCNNNNNNNGC	7 cut(s)	74, 83, 923, 961, 1319, 1322, 1349
HpyF3I	CTNAG	2 cut(s)	1317, 1554
HpySE526I	ACGT	1 cut(s)	685
Hsp92II	CATG	9 cut(s)	234, 288, 327, 331, 892, 917, 955, 1230, 1595
HspAI	GCGC	5 cut(s)	68, 962, 1296, 1352, 1516
Ksp22I	TGATCA	1 cut(s)	1002
Kzo9I	GATC	9 cut(s)	211, 800, 832, 1002, 1126, 1237, 1330, 1480, 1597
LmnI	GCTCC	5 cut(s)	157, 350, 382, 901, 1087
Lsp1109I	GCAGC	5 cut(s)	971, 1073, 1285, 1300, 1309
LweI	GCATC	2 cut(s)	436, 817
MaeI	CTAG	3 cut(s)	30, 180, 1083
MaeII	ACGT	1 cut(s)	685
MaeIII	GTNAC	2 cut(s)	1270, 1546
MalI	GATC	9 cut(s)	213, 802, 834, 1004, 1128, 1239, 1332, 1482, 1599
MboI	GATC	9 cut(s)	211, 800, 832, 1002, 1126, 1237, 1330, 1480, 1597
MflI	RGATCY	1 cut(s)	211
MhlI	GDGCHC	1 cut(s)	906
MlyI	GAGTC	1 cut(s)	308
Mph1103I	ATGCAT	1 cut(s)	329
MroXI	GAANNNNTTC	1 cut(s)	1077
MseI	TTAA	4 cut(s)	120, 512, 1470, 1530
MslI	CAYNNNNRTG	1 cut(s)	1593
MspA1I	CMGCKG	2 cut(s)	92, 432
MspCI	CTTAAG	1 cut(s)	1469
MspR9I	CCNGG	4 cut(s)	260, 340, 971, 1154
MvaI	CCWGG	4 cut(s)	260, 340, 971, 1154
MvnI	CGCG	2 cut(s)	70, 1354
MwoI	GCNNNNNNNGC	7 cut(s)	74, 83, 923, 961, 1319, 1322, 1349
NcoI	CCATGG	1 cut(s)	888
NdeII	GATC	9 cut(s)	211, 800, 832, 1002, 1126, 1237, 1330, 1480, 1597
NlaIII	CATG	9 cut(s)	234, 288, 327, 331, 892, 917, 955, 1230, 1595
NlaIV	GGNNCC	2 cut(s)	301, 428
NsiI	ATGCAT	1 cut(s)	329
NspI	RCATGY	2 cut(s)	288, 955
PceI	AGGCCT	1 cut(s)	178
PdmI	GAANNNNTTC	1 cut(s)	1077
PfeI	GAWTC	2 cut(s)	482, 1140
PflMI	CCANNNNNTGG	1 cut(s)	1218
PkrI	GCNGC	5 cut(s)	961, 1063, 1300, 1315, 1324
PleI	GAGTC	1 cut(s)	308
PpsI	GAGTC	1 cut(s)	308
Ppu21I	YACGTR	1 cut(s)	686
PshBI	ATTAAT	1 cut(s)	120
PsiI	TTATAA	1 cut(s)	987
Psp124BI	GAGCTC	1 cut(s)	906
Psp6I	CCWGG	4 cut(s)	258, 338, 969, 1152
PspFI	CCCAGC	2 cut(s)	77, 1133
PspGI	CCWGG	4 cut(s)	258, 338, 969, 1152
PspN4I	GGNNCC	2 cut(s)	301, 428
PspPI	GGNCC	4 cut(s)	336, 427, 1150, 1585
PstNI	CAGNNNCTG	2 cut(s)	713, 830
PsuI	RGATCY	1 cut(s)	211
PvuII	CAGCTG	1 cut(s)	92
RsaI	GTAC	4 cut(s)	307, 684, 688, 1052
RsaNI	GTAC	4 cut(s)	306, 683, 687, 1051
RseI	CAYNNNNRTG	1 cut(s)	1593
SacI	GAGCTC	1 cut(s)	906
SaqAI	TTAA	4 cut(s)	120, 512, 1470, 1530
SatI	GCNGC	5 cut(s)	960, 1062, 1299, 1314, 1323
Sau3AI	GATC	9 cut(s)	211, 800, 832, 1002, 1126, 1237, 1330, 1480, 1597
Sau96I	GGNCC	4 cut(s)	336, 427, 1150, 1585
SchI	GAGTC	1 cut(s)	308
ScrFI	CCNGG	4 cut(s)	260, 340, 971, 1154
SduI	GDGCHC	1 cut(s)	906
SfaNI	GCATC	2 cut(s)	436, 817
SfcI	CTRYAG	2 cut(s)	1252, 1570
SinI	GGWCC	2 cut(s)	427, 1585
SmiMI	CAYNNNNRTG	1 cut(s)	1593
SmlI	CTYRAG	2 cut(s)	527, 1469
SmoI	CTYRAG	2 cut(s)	527, 1469
SnaBI	TACGTA	1 cut(s)	686
SseBI	AGGCCT	1 cut(s)	178
SsiI	CCGC	5 cut(s)	9, 424, 430, 808, 974
SspMI	CTAG	3 cut(s)	30, 180, 1083
SstI	GAGCTC	1 cut(s)	906
StuI	AGGCCT	1 cut(s)	178
StyD4I	CCNGG	4 cut(s)	258, 338, 969, 1152
StyI	CCWWGG	4 cut(s)	155, 633, 888, 1082
TaaI	ACNGT	4 cut(s)	296, 715, 1039, 1216
TaiI	ACGT	1 cut(s)	688
TaqI	TCGA	3 cut(s)	499, 765, 865
TatI	WGTACW	1 cut(s)	305
TfiI	GAWTC	2 cut(s)	482, 1140
Tru1I	TTAA	4 cut(s)	120, 512, 1470, 1530
Tru9I	TTAA	4 cut(s)	120, 512, 1470, 1530
TscAI	CASTG	3 cut(s)	1042, 1219, 1392
TseI	GCWGC	5 cut(s)	959, 1061, 1298, 1313, 1322
TspRI	CASTG	3 cut(s)	1042, 1219, 1392
Van91I	CCANNNNNTGG	1 cut(s)	1218
Vha464I	CTTAAG	1 cut(s)	1469
VpaK11BI	GGWCC	2 cut(s)	427, 1585
VspI	ATTAAT	1 cut(s)	120
XapI	RAATTY	4 cut(s)	506, 721, 1012, 1076
XceI	RCATGY	2 cut(s)	288, 955
XcmI	CCANNNNNNNNNTGG	1 cut(s)	325
XmaJI	CCTAGG	1 cut(s)	1082
XmnI	GAANNNNTTC	1 cut(s)	1077
XspI	CTAG	3 cut(s)	30, 180, 1083
Zsp2I	ATGCAT	1 cut(s)	329

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.