Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0002289

Physical Location & Seq

Genomic Coordinates Reverse (-)

9547689 .. 9550920

Gene Structure 3232 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g16720.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1287 bp

ATGGCGGCCTTCTCTCACCTCTCCTTCACTCCTTCACCCCCACCCACCAAACTCGGCACCTTGAACTTCAAGGTCACCTCTTGCCTCTCCCTAAAGCAATCAAATGACCAGACTCTCGCCTCCCAAAACGACGCCGTATTATTCGACAGCCTCCGTGTCGTCTTCGCCGCCGGTGGTACCGGAGGCCACATATACCCGGCCGTCGCCATAGCCGACGAGCTCAAAACCACCAACCCCAACGCCCAAATTCTGTTCTTCGGCATACCCAAAAGCATGGAAAGCAAGGCCATCCCCAACGCCGGCCACGACTTCGCCGCCGTCCCCGCCGCCAAGTTGCACCGCCCGGTTCTGTCACCCAGAAACGTCTTCCTACCCTATCACTTGATCAAGTCGATCATCTGGAGCTACTACAAATTACGGGAGTTTGATCCCCATATTGTGATCGGCACCGGCGGGTACGTATCGTTTCCGGTGGGCATTGCGGCGGCGCTGAGGGGAGTCAAGCTTGTGATTCAAGAACAGAATGCTGTTCCGGGGATAGCCAATTGGTTGCTCTCGTTTTTGGCTGAGGTTGTGTTTGTGGCGTTTAATTCTACTATTGATTGTTTCCCAAGTGCGAGTGGGAAGAAGTGTGTGGTGTGTGGGAACCCTGTGAGGTTGTCTTTGAGAAAGAATGTGTCTAAGGAGGCGGCGAGGGGGCGGTTTTTTCCAAAGTGCGGCAAGGAGGTGGAGGATGTGAAGGTGCTTTTGGTGCTTGGAGGGTCTTTAGGTGCTAATGCCATCAATATTGCTTTGTTGAATTTGTATTATCAGATGTTGTTGGAGAAGGAAGACTTGTTCATCATATGGCAGACTGGAGTGGAGGCCTATAATGAGATGGAGAGCCTTGTTAAGAACCATCCGCGGCTGCTTTTGAAGCCGTTCATGCATAAAATGGATCTGGCATATGCAGCTGCAGACCTAGTCGTTTCACGAGCTGGTGCAATGACTTGTTATGAGATCTTGGCAACTGGGAAGCCTTCCATTCTGATACCATCACCAGATGCTGATGAAGGACATCAATTCAAAAATGCCTCTTTGATGGCAGACTTGGCGGGTGCAAGGGTTATAACTGAAGATGAACTTGATTCGACAACACTTGGAAATGCAATAGAAGAGATAGTAGGGGATGAGAGTAAAATGGCAGATATGTCCGAGAGGGCTCTGAAAGCTGCAAACGTTAATGCCTCTGAAGAGATTGCACATCACGTTCTTTCTCTAGTCAGCTTATCAAGAGCAAAGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00550 ko01100 ko01502 ko04112 map00550 map01100 map01502 map04112

Pfam Domains

Protein Families

Glyco_tran_28_C Glyco_transf_28

Protein Analysis

429

Amino Acids

46.28

Weight (kDa)

7.16

Isoelectric Point (pI)

38.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_28	PF03033	53 - 193	1.7e-30	Glycosyltransferase family 28 N-terminal domain
Glyco_tran_28_C	PF04101	248 - 408	7e-36	Glycosyltransferase family 28 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002289)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73740
fragaria_vesca	FvH4_5g16720 FvH4_5g16720 FvH4_5g16720
malus_domestica	MD06G1220900.v1.1 MD14G1230300.v1.1
prunus_persica	Prupe.5G225300_v2.0.a1 Prupe.5G225300_v2.0.a1
pyrus_communis	pycom06g19680 pycom14g19160
rosa_chinensis	RchiOBHm_Chr2g0147091 RchiOBHm_Chr4g0425761 RchiOBHm_Chr5g0055191 RchiOBHm_Chr7g0179381
rosa_laevigata	RLG00000005361 RLG00000020332
rosa_multiflora	Rmu_co8392303.1_g000001 Rmu_sc0001986.1_g000009 Rmu_sc0005157.1_g000016 Rmu_sc0016328.1_g000001 Rmu_sc0021620.1_g000001
rosa_roxburghii	Rroxscaffold_180G00433370 Rroxscaffold_1G00009080 Rroxscaffold_2G00138060 Rroxscaffold_3G00274080 Rroxscaffold_4G00307130 Rroxscaffold_6G00394940 Rroxscaffold_6G00425500
rosa_rugosa	Rorug02G0167700 Rorug04G0276300 Rorug06G0423900
rosa_samantha	Rh4BG339500 Rh4CG354700 Rh4DG205700 Rh5CG449000 Rh6DG030500 Rh7AG023300 Rh7BG022900 Rh7CG024200 Rh7DG023700 Rh7DG284700
rosa_wichuraiana	Rw7G001880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1109
Acc65I	GGTACC	1 cut(s)	176
AccB1I	GGYRCC	3 cut(s)	56, 176, 446
AccII	CGCG	1 cut(s)	904
AclI	AACGTT	1 cut(s)	1218
AclWI	GGATC	2 cut(s)	422, 945
AcoI	YGGCCR	2 cut(s)	198, 301
AcsI	RAATTY	2 cut(s)	246, 799
AcuI	CTGAAG	2 cut(s)	1134, 1251
AcyI	GRCGYC	1 cut(s)	132
AfaI	GTAC	2 cut(s)	178, 458
AfiI	CCNNNNNNNGG	2 cut(s)	299, 716
AgsI	TTSAA	6 cut(s)	64, 70, 515, 799, 916, 1066
AjuI	GAANNNNNNNTTGG	2 cut(s)	731, 763
AloI	GAACNNNNNNTCC	2 cut(s)	821, 853
AluBI	AGCT	7 cut(s)	220, 405, 505, 953, 977, 1211, 1266
AluI	AGCT	7 cut(s)	220, 405, 505, 953, 977, 1211, 1266
Alw21I	GWGCWC	1 cut(s)	222
AlwI	GGATC	2 cut(s)	422, 945
AlwNI	CAGNNNCTG	1 cut(s)	1046
AoxI	GGCC	6 cut(s)	6, 184, 198, 285, 301, 864
ApeKI	GCWGC	4 cut(s)	907, 950, 953, 1211
ApoI	RAATTY	2 cut(s)	246, 799
Asp700I	GAANNNNTTC	1 cut(s)	920
Asp718I	GGTACC	1 cut(s)	176
AspLEI	GCGC	1 cut(s)	490
AsuC2I	CCSGG	3 cut(s)	197, 344, 534
AsuHPI	GGTGA	5 cut(s)	8, 27, 67, 345, 1029
BanI	GGYRCC	3 cut(s)	56, 176, 446
BanII	GRGCYC	2 cut(s)	222, 1204
BauI	CACGAG	1 cut(s)	972
BbsI	GAAGAC	3 cut(s)	154, 358, 837
Bbv12I	GWGCWC	1 cut(s)	222
BbvCI	CCTCAGC	2 cut(s)	491, 567
BbvI	GCAGC	4 cut(s)	894, 940, 962, 1198
BccI	CCATC	6 cut(s)	296, 788, 871, 906, 1042, 1075
BceAI	ACGGC	4 cut(s)	119, 185, 302, 904
BclI	TGATCA	1 cut(s)	384
BcnI	CCSGG	3 cut(s)	197, 344, 534
BfaI	CTAG	2 cut(s)	962, 1259
BfmI	CTRYAG	1 cut(s)	954
BfoI	RGCGCY	1 cut(s)	491
BglII	AGATCT	1 cut(s)	999
Bme1390I	CCNGG	3 cut(s)	197, 344, 534
BmiI	GGNNCC	4 cut(s)	58, 178, 448, 647
BmrFI	CCNGG	3 cut(s)	197, 344, 534
BmrI	ACTGGG	1 cut(s)	1020
BmsI	GCATC	1 cut(s)	1033
BmuI	ACTGGG	1 cut(s)	1020
BpiI	GAAGAC	3 cut(s)	154, 358, 837
BpmI	CTGGAG	2 cut(s)	421, 876
Bpu10I	CCTNAGC	2 cut(s)	491, 567
BpuMI	CCSGG	3 cut(s)	197, 344, 534
BsaAI	YACGTR	1 cut(s)	460
BsaHI	GRCGYC	1 cut(s)	132
BsaJI	CCNNGG	2 cut(s)	533, 902
BsaWI	WCCGGW	2 cut(s)	179, 469
Bsc4I	CCNNNNNNNGG	2 cut(s)	299, 716
Bse118I	RCCGGY	3 cut(s)	170, 299, 449
Bse1I	ACTGG	2 cut(s)	859, 1015
Bse3DI	GCAATG	2 cut(s)	477, 990
BseDI	CCNNGG	2 cut(s)	533, 902
BseGI	GGATG	4 cut(s)	288, 739, 898, 1174
BseLI	CCNNNNNNNGG	2 cut(s)	299, 716
BseMI	GCAATG	2 cut(s)	477, 990
BseMII	CTCAG	2 cut(s)	482, 558
BseNI	ACTGG	2 cut(s)	859, 1015
BseX3I	CGGCCG	1 cut(s)	198
BseXI	GCAGC	4 cut(s)	894, 940, 962, 1198
Bsh1236I	CGCG	1 cut(s)	904
Bsh1285I	CGRYCG	1 cut(s)	201
BshFI	GGCC	6 cut(s)	8, 186, 200, 287, 303, 866
BshNI	GGYRCC	3 cut(s)	56, 176, 446
BsiEI	CGRYCG	1 cut(s)	201
BsiHKAI	GWGCWC	1 cut(s)	222
BsiSI	CCGG	8 cut(s)	171, 180, 197, 300, 344, 450, 470, 533
BslFI	GGGAC	1 cut(s)	305
BslI	CCNNNNNNNGG	2 cut(s)	299, 716
BsmFI	GGGAC	1 cut(s)	305
BsmI	GAATGC	1 cut(s)	529
BsnI	GGCC	6 cut(s)	8, 186, 200, 287, 303, 866
Bsp1286I	GDGCHC	2 cut(s)	222, 1204
Bsp143I	GATC	6 cut(s)	384, 393, 427, 441, 937, 999
BspANI	GGCC	6 cut(s)	8, 186, 200, 287, 303, 866
BspCNI	CTCAG	2 cut(s)	483, 559
BspFNI	CGCG	1 cut(s)	904
BspLI	GGNNCC	4 cut(s)	58, 178, 448, 647
BspMAI	CTGCAG	1 cut(s)	958
BspPI	GGATC	2 cut(s)	422, 945
BspT107I	GGYRCC	3 cut(s)	56, 176, 446
BsrDI	GCAATG	2 cut(s)	477, 990
BsrFI	RCCGGY	3 cut(s)	170, 299, 449
BsrI	ACTGG	2 cut(s)	859, 1015
BssAI	RCCGGY	3 cut(s)	170, 299, 449
BssECI	CCNNGG	2 cut(s)	533, 902
BssMI	GATC	6 cut(s)	384, 393, 427, 441, 937, 999
BssNI	GRCGYC	1 cut(s)	132
BssSI	CACGAG	1 cut(s)	972
Bst2BI	CACGAG	1 cut(s)	972
Bst6I	CTCTTC	2 cut(s)	1149, 1227
BstACI	GRCGYC	1 cut(s)	132
BstBAI	YACGTR	1 cut(s)	460
BstC8I	GCNNGC	1 cut(s)	301
BstDEI	CTNAG	3 cut(s)	491, 567, 681
BstDSI	CCRYGG	1 cut(s)	902
BstEII	GGTNACC	1 cut(s)	73
BstF5I	GGATG	4 cut(s)	288, 739, 898, 1174
BstFNI	CGCG	1 cut(s)	904
BstH2I	RGCGCY	1 cut(s)	491
BstHHI	GCGC	1 cut(s)	490
BstKTI	GATC	6 cut(s)	387, 396, 430, 444, 940, 1002
BstMBI	GATC	6 cut(s)	384, 393, 427, 441, 937, 999
BstMCI	CGRYCG	1 cut(s)	201
BstMWI	GCNNNNNNNGC	8 cut(s)	279, 323, 751, 916, 925, 950, 1091, 1208
BstPI	GGTNACC	1 cut(s)	73
BstSCI	CCNGG	3 cut(s)	195, 342, 532
BstSFI	CTRYAG	1 cut(s)	954
BstSNI	TACGTA	1 cut(s)	460
BstUI	CGCG	1 cut(s)	904
BstV1I	GCAGC	4 cut(s)	894, 940, 962, 1198
BstV2I	GAAGAC	3 cut(s)	154, 358, 837
BstX2I	RGATCY	2 cut(s)	937, 999
BstXI	CCANNNNNNTGG	1 cut(s)	274
BstYI	RGATCY	2 cut(s)	937, 999
BstZI	CGGCCG	1 cut(s)	198
BsuRI	GGCC	6 cut(s)	8, 186, 200, 287, 303, 866
BtgI	CCRYGG	1 cut(s)	902
BtsCI	GGATG	4 cut(s)	288, 739, 898, 1174
Cac8I	GCNNGC	1 cut(s)	301
CaiI	CAGNNNCTG	1 cut(s)	1046
CfoI	GCGC	1 cut(s)	490
Cfr10I	RCCGGY	3 cut(s)	170, 299, 449
Cfr42I	CCGCGG	1 cut(s)	905
CseI	GACGC	1 cut(s)	140
Csp6I	GTAC	2 cut(s)	177, 457
CviAII	CATG	2 cut(s)	274, 925
CviQI	GTAC	2 cut(s)	177, 457
DdeI	CTNAG	3 cut(s)	491, 567, 681
DpnI	GATC	6 cut(s)	386, 395, 429, 443, 939, 1001
DpnII	GATC	6 cut(s)	384, 393, 427, 441, 937, 999
EaeI	YGGCCR	2 cut(s)	198, 301
EagI	CGGCCG	1 cut(s)	198
Eam1104I	CTCTTC	2 cut(s)	1149, 1227
EarI	CTCTTC	2 cut(s)	1149, 1227
Ecl136II	GAGCTC	1 cut(s)	220
EclXI	CGGCCG	1 cut(s)	198
Eco105I	TACGTA	1 cut(s)	460
Eco147I	AGGCCT	1 cut(s)	866
Eco24I	GRGCYC	2 cut(s)	222, 1204
Eco52I	CGGCCG	1 cut(s)	198
Eco53kI	GAGCTC	1 cut(s)	220
Eco57I	CTGAAG	2 cut(s)	1134, 1251
Eco91I	GGTNACC	1 cut(s)	73
EcoICRI	GAGCTC	1 cut(s)	220
EcoO65I	GGTNACC	1 cut(s)	73
EcoT22I	ATGCAT	1 cut(s)	930
EcoT38I	GRGCYC	2 cut(s)	222, 1204
FaeI	CATG	2 cut(s)	277, 928
FalI	AAGNNNNNCTT	4 cut(s)	818, 850, 1107, 1139
FaqI	GGGAC	1 cut(s)	305
FatI	CATG	2 cut(s)	273, 924
FauI	CCCGC	3 cut(s)	331, 446, 1087
FauNDI	CATATG	2 cut(s)	845, 946
FbaI	TGATCA	1 cut(s)	384
FokI	GGATG	4 cut(s)	275, 746, 885, 1181
FriOI	GRGCYC	2 cut(s)	222, 1204
FspBI	CTAG	2 cut(s)	962, 1259
GlaI	GCGC	1 cut(s)	489
GsuI	CTGGAG	2 cut(s)	421, 876
HaeII	RGCGCY	1 cut(s)	491
HaeIII	GGCC	6 cut(s)	8, 186, 200, 287, 303, 866
HapII	CCGG	8 cut(s)	171, 180, 197, 300, 344, 450, 470, 533
HgaI	GACGC	1 cut(s)	140
HhaI	GCGC	1 cut(s)	490
Hin1I	GRCGYC	1 cut(s)	132
Hin1II	CATG	2 cut(s)	277, 928
Hin6I	GCGC	1 cut(s)	488
HinP1I	GCGC	1 cut(s)	488
HindIII	AAGCTT	1 cut(s)	503
HinfI	GANTC	4 cut(s)	112, 498, 511, 1127
HpaII	CCGG	8 cut(s)	171, 180, 197, 300, 344, 450, 470, 533
HphI	GGTGA	5 cut(s)	8, 27, 67, 345, 1029
Hpy188I	TCNGA	5 cut(s)	813, 1029, 1195, 1206, 1231
Hpy188III	TCNNGA	4 cut(s)	400, 515, 972, 1272
Hpy99I	CGWCG	3 cut(s)	134, 206, 218
HpyAV	CCTTC	7 cut(s)	19, 34, 42, 733, 820, 1029, 1046
HpyCH4IV	ACGT	4 cut(s)	363, 459, 1218, 1248
HpyCH4V	TGCA	9 cut(s)	337, 928, 950, 956, 983, 1100, 1148, 1214, 1241
HpyF10VI	GCNNNNNNNGC	8 cut(s)	279, 323, 751, 916, 925, 950, 1091, 1208
HpyF3I	CTNAG	3 cut(s)	491, 567, 681
HpySE526I	ACGT	4 cut(s)	363, 459, 1218, 1248
Hsp92I	GRCGYC	1 cut(s)	132
Hsp92II	CATG	2 cut(s)	277, 928
HspAI	GCGC	1 cut(s)	488
KpnI	GGTACC	1 cut(s)	180
KroI	GCCGGC	1 cut(s)	299
KroNI	GCCGGC	1 cut(s)	301
Ksp22I	TGATCA	1 cut(s)	384
KspI	CCGCGG	1 cut(s)	905
Kzo9I	GATC	6 cut(s)	384, 393, 427, 441, 937, 999
LmnI	GCTCC	1 cut(s)	402
Lsp1109I	GCAGC	4 cut(s)	894, 940, 962, 1198
LweI	GCATC	1 cut(s)	1033
MaeI	CTAG	2 cut(s)	962, 1259
MaeII	ACGT	4 cut(s)	363, 459, 1218, 1248
MaeIII	GTNAC	2 cut(s)	73, 351
MalI	GATC	6 cut(s)	386, 395, 429, 443, 939, 1001
MboI	GATC	6 cut(s)	384, 393, 427, 441, 937, 999
MboII	GAAGA	8 cut(s)	154, 247, 358, 637, 842, 1127, 1166, 1244
MfeI	CAATTG	1 cut(s)	544
MflI	RGATCY	2 cut(s)	937, 999
MhlI	GDGCHC	2 cut(s)	222, 1204
MluCI	AATT	6 cut(s)	246, 413, 544, 589, 799, 1061
MlyI	GAGTC	2 cut(s)	106, 507
MmeI	TCCRAC	1 cut(s)	801
Mph1103I	ATGCAT	1 cut(s)	930
MroNI	GCCGGC	1 cut(s)	299
MroXI	GAANNNNTTC	1 cut(s)	920
MseI	TTAA	3 cut(s)	588, 891, 1221
MspA1I	CMGCKG	2 cut(s)	904, 953
MspI	CCGG	8 cut(s)	171, 180, 197, 300, 344, 450, 470, 533
MspR9I	CCNGG	3 cut(s)	197, 344, 534
MunI	CAATTG	1 cut(s)	544
Mva1269I	GAATGC	1 cut(s)	529
MvnI	CGCG	1 cut(s)	904
MwoI	GCNNNNNNNGC	8 cut(s)	279, 323, 751, 916, 925, 950, 1091, 1208
NaeI	GCCGGC	1 cut(s)	301
NciI	CCSGG	3 cut(s)	197, 344, 534
NdeI	CATATG	2 cut(s)	845, 946
NdeII	GATC	6 cut(s)	384, 393, 427, 441, 937, 999
NgoMIV	GCCGGC	1 cut(s)	299
NlaIII	CATG	2 cut(s)	277, 928
NlaIV	GGNNCC	4 cut(s)	58, 178, 448, 647
NmeAIII	GCCGAG	1 cut(s)	33
NmuCI	GTSAC	2 cut(s)	73, 351
NsiI	ATGCAT	1 cut(s)	930
PceI	AGGCCT	1 cut(s)	866
PcsI	WCGNNNNNNNCGW	2 cut(s)	210, 303
PctI	GAATGC	1 cut(s)	529
PdiI	GCCGGC	1 cut(s)	301
PdmI	GAANNNNTTC	1 cut(s)	920
PfeI	GAWTC	2 cut(s)	511, 1127
PflFI	GACNNNGTC	1 cut(s)	962
PleI	GAGTC	2 cut(s)	106, 506
PpsI	GAGTC	2 cut(s)	106, 506
Ppu21I	YACGTR	1 cut(s)	460
PsiI	TTATAA	1 cut(s)	1109
Psp124BI	GAGCTC	1 cut(s)	222
Psp1406I	AACGTT	1 cut(s)	1218
PspEI	GGTNACC	1 cut(s)	73
PspN4I	GGNNCC	4 cut(s)	58, 178, 448, 647
PstI	CTGCAG	1 cut(s)	958
PstNI	CAGNNNCTG	1 cut(s)	1046
PsuI	RGATCY	2 cut(s)	937, 999
PsyI	GACNNNGTC	1 cut(s)	962
PvuII	CAGCTG	1 cut(s)	953
RsaI	GTAC	2 cut(s)	178, 458
RsaNI	GTAC	2 cut(s)	177, 457
SacI	GAGCTC	1 cut(s)	222
SacII	CCGCGG	1 cut(s)	905
SaqAI	TTAA	3 cut(s)	588, 891, 1221
Sau3AI	GATC	6 cut(s)	384, 393, 427, 441, 937, 999
SchI	GAGTC	2 cut(s)	106, 507
ScrFI	CCNGG	3 cut(s)	197, 344, 534
SduI	GDGCHC	2 cut(s)	222, 1204
SfaNI	GCATC	1 cut(s)	1033
SfcI	CTRYAG	1 cut(s)	954
Sfr303I	CCGCGG	1 cut(s)	905
SgrAI	CRCCGGYG	2 cut(s)	170, 449
SgrBI	CCGCGG	1 cut(s)	905
SnaBI	TACGTA	1 cut(s)	460
Sse9I	AATT	6 cut(s)	246, 413, 544, 589, 799, 1061
SseBI	AGGCCT	1 cut(s)	866
SspI	AATATT	1 cut(s)	787
SspMI	CTAG	2 cut(s)	962, 1259
SstI	GAGCTC	1 cut(s)	222
StuI	AGGCCT	1 cut(s)	866
StyD4I	CCNGG	3 cut(s)	195, 342, 532
TaiI	ACGT	4 cut(s)	366, 462, 1221, 1251
TaqI	TCGA	3 cut(s)	144, 392, 1130
TasI	AATT	6 cut(s)	246, 413, 544, 589, 799, 1061
TauI	GCSGC	9 cut(s)	8, 170, 317, 329, 485, 488, 692, 720, 907
TfiI	GAWTC	2 cut(s)	511, 1127
Tru1I	TTAA	3 cut(s)	588, 891, 1221
Tru9I	TTAA	3 cut(s)	588, 891, 1221
TseFI	GTSAC	2 cut(s)	73, 351
TseI	GCWGC	4 cut(s)	907, 950, 953, 1211
Tsp45I	GTSAC	2 cut(s)	73, 351
TspDTI	ATGAA	4 cut(s)	829, 913, 1065, 1134
TspGWI	ACGGA	1 cut(s)	143
Tth111I	GACNNNGTC	1 cut(s)	962
XapI	RAATTY	2 cut(s)	246, 799
XmnI	GAANNNNTTC	1 cut(s)	920
XspI	CTAG	2 cut(s)	962, 1259
Zsp2I	ATGCAT	1 cut(s)	930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.