Rorug06G0423900

UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002289
Physical Location & Seq
Forward (+)
56187139 .. 56190708
3570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0423900.1

Sequence Viewer

Length: 3405 bp
ATGGCAACGGCTAGTTTCTTCTTTCTGATCATCCCAATGCTGTACTTCACCGCCGCATTGACCGCCGGTCAACTTTTACTCTCCGAGATCCAAGCCTTAACCTCCTTCAAGCTCAACCTCCACGACCCGCTCGGCGCCCTCAACGGCTGGGACTCCTCCACGCCTTCCGCCCCCTGCGACTGGCGCGGCGTCGTTTGCTTCAACAACCAAGTCCGGGAACTCAGCCTGCCTCGCCTCCACCTCGCGGGTCGACTCGGCGACCAACTCGCCAGCCTCCGTGACCTCCGCAAGCTCAACCTCCACACCAACAACTTCAACGGCTCCATTCCTCCCTCCCTCTCCCAATGCGCCTTGCTACGCACCGTTTACTTGCACGGCAACTCACTCTCCGGCGACCTCCCGCCGGCGCTTCTCAACCTCACCAACCTCCAAATCCTCAACCTCGCTCACAACTCCCTCTCCGGCAGAATCTCCGGCGACATTCCGGCCAGCCTCCGGTACCTTGATCTTTCCTCCAATGCATTCACCGGGGACGTTCCGGTGAACTTCTCCGCCAACTCGCAGCTCCAGCTCGTCAATTTTTCCTTCAATCAGTTCACCGGCGGCGTTCCGCCGACCATCGGCCGGCTCCAGATGCTTGAGTATCTGTGGCTGGACTCGAACCAGCTGACCGGCACGCTTCCTTCGGCTATTTCCAACTGCTCCTCCCTTGTACATTTCAGCGCCGACGACAACGCGCTCCGAGGACTAATTCCGGCGACCATCGGCGCCATTCCGAAGCTTCAGGTGGTTTCTTTAGCTCGGAACAGCTTATCAGGCTCAGTCCCGATAGCTTTATTGTGCAATGGGAATGTTGACTCTTCTTCACTGAGGATAGTAGATCTAGGGTTCAATGCGCTCACGGGAATTGTGCAGCCGCAAAATGGAAGGTGTTGGAGTATTCTGGAGGTTCTGGTTCTTAAAAGCAATCGCATTGGCGGCGCGTTCCCAAATTGGTTGAGTAATGTGACTAGGCTGAGGGTTTTCGATGTTGCTGGAAACTCGTTTTCGGGGGGATTGCCGGCTGAAATCGGCAATCTTTTCCGGTTGGAAGAACTCCGGGTGGGTAATAATTCCCTGAGCGGTGAAATTCCTACGGAGATTGCAAAATGCAGTTTGTTACAGGCTCTTGATCTTGAAGGTAACCGCTTTGAGGGCCAGTTTCCTTTGTTTCTGGGTGGAATGAGGAGTTTAAAGGTGTTGTCTTTGGGTGGGAATTTAATCTCGGGTACCATCCCGTCGAGTTTGGGAGCACTCTCGGGGCTAGAATCCTTGAATCTGAGCGGTAATAATCTGACAGGGGAAGTTCCAGTTGAGCTCATGCAGCTAAGCAACTTGACTAGCTTGAACCTGAGTAATAACAAGTTTTCCGGTGAGGTTCTTGGGAATGTAGGAGCATTGAGGAGCTTGCAGGGTTTGAATTTAAGCAATTGTGGGCTTTCAGGTAGCGTCCCAGCGAGTATTGGAAGTCTGATGAGGCTCGCTACACTGGATTTGAGTAAACAAAAGCTCTCTGGTGAGTTGCCTACTGAGATTTTTGGGCTGCCAAATTTACAAGTGGTTGCTTTGCAAGACAATCAGTTGTCTGGGGCTGTCCCTCAAGGTTTTAGTAGCTTGACTGGTTTAGTGTACCTAAATCTCTCCTCCAATGCCTTTACTGGTGGTATTCCAGCCACGTATGGATATCTACGGTCACTAACCGTTCTATCTCTGTCAAAGAATCGCATTTCCGGTGTGATCCCACCAGAGCTTGGAGATTGTTTGAACCTTCAAGTACTTGAGCTTAGTTCTAATCAACTGGGGGACAAAATTCCAGTTGATATCTCTCGGCTATCTAGTTTGGAAGAGCTGGATTTAGGTCATAACAAATTGACAGGTGAAATACCAGAGGAAATTTCCAAATGCTCTGCTCTGACTTCATTATTGTTAGAGGAAAATCAACTTTCAGGGCCAATACCAAACTCCTTGCCTTTGTTAGTGAACCTTGTGGTGTTAGATCTTTCCTCAAACCAGTTGAGTGGAGCAATCCCGGCAAATATTTCACTCATTCCTCACTTGAAGTCCTTGAATTTGTCTAACAACAACCTATCTGGTAAGATTCCAGAGCTACTGGGTTCTCAATTCAATGGTTCTGTCTTTGCAATGAACCCGAAATTATGTGGGAAACCATTGGACAGAGAATGTGGAGATGCGAAAAGGAGAAGGAGAAACAGGTTGCTTTTATTAATTGGGGTGGCTGCTGGTGGAGCTTTGCTTCTGTTAATGTGTTGTTGTGGCTATGTCTACAGCCTCTTCCGCTGGCGTAAGAAGCTTAGAGAAATGGTAACTGGGGAGAAGAAGCGTCCCAGTCCAGGTCGTGCAAGCTCAGGCGGAGAGAGATCTGGTCGCGGAAGTGGAGACAATGGAGGGCCAAAGCTTGTTATGTTCAATACTAAGATTACATATGCAGAAGCATTAGAAGCAACAAGACAATTTGATGAGGAAAATGTGTTAAGCAGGGGAAAGTACGGACTGGTATTCAAGGCTTCATTCCCAGATGGAATGGTGCTCTCAATTCGACGCCTTCCTGCTTCTTCTCTTGGTGTGCTCGATGAGGAGACGTTCCACAAAGAAGCCGAAGCTCTTGGCAAGGTGAAGCATCGGAACTTAACAGTTCTGCGAGGCTACTATGCCGGTCCTGATGTGAGGCTACTTGTATACGACTACATGCCTAATGGAAACTTAGCCACTCTGCTCCAAGAGGCTTCTCACCAAGATGGTCATGTACTAAATTGGCCAATGCGGCATCTCATTGCACTTGGCATTGCTAGAGGCATAGCTTTCCTGCACTCAGTCTCAATGGTTCATGGTGATGTGAAGCCGCAAAATGTTCTCTTTGACGCTGATTTTGAAGCCCACTTATCGGAATTTGGATTAGACCGGTTAACCATAGTCTCACCAAAAGCCTCCTCATCATCATCTGCCGCAATTGGTACATTAGGCTATGTGTCACCCGAAGCAGCACTGACCGGGCAGGCAACAAAAGAAGCCGATGTGTATAGTTTCGGAATTGTCCTGTTGGAGATCCTAACCGGAAGGAAGCCAGTGATGTTTACTGAAGAGGAAGACATAGTGAAATGGGTGAAGAAGCAACTACAAAGAGGTCAAATCACAGAGCTGCTAGAGCCTGGACTACTTGAGCTAGACCCGGAGTCATCAGAATCAGAAGAGTTCTTATTGGGAGTAAAAGTTGGACTACTCTGCACCGCACCTGATCCACTTGATAGACCCCTCATGGCTGACATTGTGTTCATGCTTGAAGGTTGCCGCGTCGGACCGGACATACCATCGTCGGCTGATCCAACTTCACTACCTTCACCGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00550 ko01100 ko01502 ko04112 map00550 map01100 map01502 map04112
Pfam Domains
Protein Families
Glyco_tran_28_C Glyco_transf_28

Protein Analysis

1134

Amino Acids

121.5

Weight (kDa)

5.98

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 66 4e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 333 - 486 2.5e-11 Leucine-rich repeat region
LRR_8 PF13855 409 - 469 4.1e-09 Leucine rich repeat
LRR_14 PF23598 482 - 610 1e-10 Leucine-rich repeat region
LRR_8 PF13855 506 - 564 9.3e-08 Leucine rich repeat
LRR_4 PF12799 578 - 615 9.3e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 622 - 661 6.2e-06 Leucine rich repeat
Pkinase PF00069 840 - 1060 4.2e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 842 - 1059 5.3e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 498, 1268
AccB1I GGYRCC 4 cut(s) 134, 498, 767, 1268
AccB7I CCANNNNNTGG 1 cut(s) 1790
AccBSI CCGCTC 3 cut(s) 130, 1122, 1323
AccI GTMKAC 3 cut(s) 250, 2322, 2736
AccII CGCG 6 cut(s) 186, 245, 737, 983, 2427, 3348
AclWI GGATC 5 cut(s) 82, 1771, 3097, 3287, 3371
AcoI YGGCCR 3 cut(s) 486, 622, 2813
AcsI RAATTY 8 cut(s) 1128, 1255, 1459, 1588, 1848, 1932, 2107, 2945
AcuI CTGAAG 2 cut(s) 767, 3156
AcyI GRCGYC 4 cut(s) 135, 189, 768, 2599
AfaI GTAC 9 cut(s) 44, 500, 714, 1270, 1670, 1815, 2546, 2805, 3013
AgeI ACCGGT 1 cut(s) 2958
AhdI GACNNNNNGTC 2 cut(s) 66, 3229
AjnI CCWGG 2 cut(s) 2389, 3205
Alw21I GWGCWC 4 cut(s) 1294, 1359, 2589, 2628
Alw26I GTCTC 4 cut(s) 2430, 2630, 2878, 2977
AlwI GGATC 5 cut(s) 82, 1771, 3097, 3287, 3371
Ama87I CYCGRG 2 cut(s) 1264, 1297
AoxI GGCC 7 cut(s) 486, 622, 1195, 1988, 2447, 2813, 3399
ApeKI GCWGC 7 cut(s) 562, 913, 1363, 1582, 2276, 3038, 3196
ApoI RAATTY 8 cut(s) 1128, 1255, 1459, 1588, 1848, 1932, 2107, 2945
ArsI GACNNNNNNTTYG 2 cut(s) 2909, 2941
AseI ATTAAT 1 cut(s) 2264
AsiGI ACCGGT 1 cut(s) 2958
Asp718I GGTACC 2 cut(s) 498, 1268
AspLEI GCGC 9 cut(s) 137, 186, 350, 409, 725, 739, 770, 898, 983
AspS9I GGNCC 5 cut(s) 1195, 1988, 2447, 2714, 3353
AsuC2I CCSGG 6 cut(s) 215, 529, 1100, 2069, 3049, 3227
AvaI CYCGRG 2 cut(s) 1264, 1297
AvaII GGWCC 2 cut(s) 2714, 3353
BalI TGGCCA 1 cut(s) 2815
BanI GGYRCC 4 cut(s) 134, 498, 767, 1268
BanII GRGCYC 1 cut(s) 1359
BbsI GAAGAC 1 cut(s) 3150
Bbv12I GWGCWC 4 cut(s) 1294, 1359, 2589, 2628
BbvCI CCTCAGC 1 cut(s) 1016
BbvI GCAGC 7 cut(s) 574, 925, 1375, 1569, 2263, 3050, 3183
BccI CCATC 6 cut(s) 626, 770, 1280, 2570, 2789, 3373
BceAI ACGGC 4 cut(s) 24, 160, 334, 391
BciT130I CCWGG 2 cut(s) 2391, 3207
BclI TGATCA 1 cut(s) 27
BcnI CCSGG 6 cut(s) 215, 529, 1100, 2069, 3049, 3227
BcoDI GTCTC 4 cut(s) 2430, 2630, 2878, 2977
BfaI CTAG 9 cut(s) 12, 884, 1011, 1304, 1380, 1875, 2847, 3200, 3221
BfmI CTRYAG 1 cut(s) 2323
BfoI RGCGCY 4 cut(s) 138, 410, 726, 771
BglI GCCNNNNNGGC 1 cut(s) 2821
BglII AGATCT 3 cut(s) 880, 2035, 2417
BlpI GCTNAGC 1 cut(s) 1367
BmcAI AGTACT 1 cut(s) 1815
Bme1390I CCNGG 8 cut(s) 215, 529, 1100, 2069, 2391, 3049, 3207, 3227
Bme18I GGWCC 2 cut(s) 2714, 3353
BmeRI GACNNNNNGTC 2 cut(s) 66, 3229
BmeT110I CYCGRG 2 cut(s) 1264, 1297
BmgT120I GGNCC 5 cut(s) 1195, 1988, 2447, 2714, 3353
BmiI GGNNCC 6 cut(s) 136, 322, 500, 629, 769, 1270
BmrFI CCNGG 8 cut(s) 215, 529, 1100, 2069, 2391, 3049, 3207, 3227
BmrI ACTGGG 4 cut(s) 1847, 2159, 2376, 2379
BmsI GCATC 4 cut(s) 624, 2218, 2686, 2833
BmuI ACTGGG 4 cut(s) 1847, 2159, 2376, 2379
BpiI GAAGAC 1 cut(s) 3150
BpmI CTGGAG 3 cut(s) 551, 614, 965
Bpu10I CCTNAGC 3 cut(s) 1016, 1118, 2404
Bpu1102I GCTNAGC 1 cut(s) 1367
BpuEI CTTGAG 4 cut(s) 659, 1623, 1838, 3236
BpuMI CCSGG 6 cut(s) 215, 529, 1100, 2069, 3049, 3227
BsaAI YACGTR 1 cut(s) 1716
BsaHI GRCGYC 4 cut(s) 135, 189, 768, 2599
BsaJI CCNNGG 2 cut(s) 528, 742
BsaWI WCCGGW 8 cut(s) 495, 538, 1083, 1409, 1769, 2958, 3110, 3355
BsaXI ACNNNNNCTCC 8 cut(s) 371, 401, 443, 473, 689, 719, 3252, 3282
Bse118I RCCGGY 9 cut(s) 65, 403, 599, 624, 671, 1060, 2711, 2958, 3397
Bse3DI GCAATG 4 cut(s) 850, 2187, 2829, 2841
BseBI CCWGG 2 cut(s) 2391, 3207
BseDI CCNNGG 2 cut(s) 528, 742
BseGI GGATG 2 cut(s) 30, 1272
BseMI GCAATG 4 cut(s) 850, 2187, 2829, 2841
BseRI GAGGAG 7 cut(s) 145, 694, 1240, 1456, 1672, 2648, 2977
BseX3I CGGCCG 1 cut(s) 622
BseXI GCAGC 7 cut(s) 574, 925, 1375, 1569, 2263, 3050, 3183
BseYI CCCAGC 2 cut(s) 147, 1492
BsgI GTGCAG 3 cut(s) 932, 2849, 3265
Bsh1236I CGCG 6 cut(s) 186, 245, 737, 983, 2427, 3348
Bsh1285I CGRYCG 1 cut(s) 625
BshFI GGCC 7 cut(s) 488, 624, 1197, 1990, 2449, 2815, 3401
BshNI GGYRCC 4 cut(s) 134, 498, 767, 1268
BshTI ACCGGT 1 cut(s) 2958
BsiEI CGRYCG 1 cut(s) 625
BsiHKAI GWGCWC 4 cut(s) 1294, 1359, 2589, 2628
BsiHKCI CYCGRG 2 cut(s) 1264, 1297
BslFI GGGAC 7 cut(s) 164, 545, 809, 1475, 1619, 1856, 2367
BsmAI GTCTC 4 cut(s) 2430, 2630, 2878, 2977
BsmBI CGTCTC 1 cut(s) 2630
BsmFI GGGAC 7 cut(s) 164, 545, 809, 1475, 1619, 1856, 2367
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 7 cut(s) 488, 624, 1197, 1990, 2449, 2815, 3401
BsoBI CYCGRG 2 cut(s) 1264, 1297
Bsp1286I GDGCHC 4 cut(s) 1294, 1359, 2589, 2628
Bsp1407I TGTACA 1 cut(s) 712
Bsp1720I GCTNAGC 1 cut(s) 1367
BspANI GGCC 7 cut(s) 488, 624, 1197, 1990, 2449, 2815, 3401
BspFNI CGCG 6 cut(s) 186, 245, 737, 983, 2427, 3348
BspLI GGNNCC 6 cut(s) 136, 322, 500, 629, 769, 1270
BspPI GGATC 5 cut(s) 82, 1771, 3097, 3287, 3371
BspQI GCTCTTC 1 cut(s) 1878
BspT107I GGYRCC 4 cut(s) 134, 498, 767, 1268
BsrBI CCGCTC 3 cut(s) 130, 1122, 1323
BsrDI GCAATG 4 cut(s) 850, 2187, 2829, 2841
BsrFI RCCGGY 9 cut(s) 65, 403, 599, 624, 671, 1060, 2711, 2958, 3397
BsrGI TGTACA 1 cut(s) 712
BssAI RCCGGY 9 cut(s) 65, 403, 599, 624, 671, 1060, 2711, 2958, 3397
BssECI CCNNGG 2 cut(s) 528, 742
BssNAI GTATAC 1 cut(s) 2737
BssNI GRCGYC 4 cut(s) 135, 189, 768, 2599
Bst1107I GTATAC 1 cut(s) 2737
Bst2UI CCWGG 2 cut(s) 2391, 3207
Bst4CI ACNGT 4 cut(s) 364, 1731, 1741, 2692
Bst6I CTCTTC 5 cut(s) 865, 1878, 2336, 3132, 3240
BstACI GRCGYC 4 cut(s) 135, 189, 768, 2599
BstAUI TGTACA 1 cut(s) 712
BstBAI YACGTR 1 cut(s) 1716
BstEII GGTNACC 1 cut(s) 1181
BstF5I GGATG 2 cut(s) 30, 1272
BstFNI CGCG 6 cut(s) 186, 245, 737, 983, 2427, 3348
BstH2I RGCGCY 4 cut(s) 138, 410, 726, 771
BstHHI GCGC 9 cut(s) 137, 186, 350, 409, 725, 739, 770, 898, 983
BstMAI GTCTC 4 cut(s) 2430, 2630, 2878, 2977
BstMCI CGRYCG 1 cut(s) 625
BstNI CCWGG 2 cut(s) 2391, 3207
BstNSI RCATGY 1 cut(s) 2749
BstPI GGTNACC 1 cut(s) 1181
BstSCI CCNGG 8 cut(s) 213, 527, 1098, 2067, 2389, 3047, 3205, 3225
BstSFI CTRYAG 1 cut(s) 2323
BstUI CGCG 6 cut(s) 186, 245, 737, 983, 2427, 3348
BstV1I GCAGC 7 cut(s) 574, 925, 1375, 1569, 2263, 3050, 3183
BstV2I GAAGAC 1 cut(s) 3150
BstX2I RGATCY 5 cut(s) 87, 880, 2035, 2417, 3102
BstXI CCANNNNNNTGG 1 cut(s) 2057
BstYI RGATCY 5 cut(s) 87, 880, 2035, 2417, 3102
BstZ17I GTATAC 1 cut(s) 2737
BstZI CGGCCG 1 cut(s) 622
BsuRI GGCC 7 cut(s) 488, 624, 1197, 1990, 2449, 2815, 3401
BtsCI GGATG 2 cut(s) 30, 1272
BtsIMutI CAGTG 4 cut(s) 866, 1526, 3041, 3129
CfoI GCGC 9 cut(s) 137, 186, 350, 409, 725, 739, 770, 898, 983
Cfr10I RCCGGY 9 cut(s) 65, 403, 599, 624, 671, 1060, 2711, 2958, 3397
Cfr13I GGNCC 5 cut(s) 1195, 1988, 2447, 2714, 3353
CpoI CGGWCCG 1 cut(s) 3353
CseI GACGC 6 cut(s) 178, 1477, 2369, 2607, 2927, 3337
Csp6I GTAC 9 cut(s) 43, 499, 713, 1269, 1669, 1814, 2545, 2804, 3012
CspAI ACCGGT 1 cut(s) 2958
CspI CGGWCCG 1 cut(s) 3353
CviAII CATG 6 cut(s) 1360, 2746, 2801, 2885, 3313, 3331
CviQI GTAC 9 cut(s) 43, 499, 713, 1269, 1669, 1814, 2545, 2804, 3012
DinI GGCGCC 2 cut(s) 136, 769
DraI TTTAAA 1 cut(s) 1233
DriI GACNNNNNGTC 2 cut(s) 66, 3229
EaeI YGGCCR 3 cut(s) 486, 622, 2813
EagI CGGCCG 1 cut(s) 622
Eam1104I CTCTTC 5 cut(s) 865, 1878, 2336, 3132, 3240
Eam1105I GACNNNNNGTC 2 cut(s) 66, 3229
EarI CTCTTC 5 cut(s) 865, 1878, 2336, 3132, 3240
EciI GGCGGA 4 cut(s) 157, 541, 600, 2424
Ecl136II GAGCTC 1 cut(s) 1357
EclXI CGGCCG 1 cut(s) 622
Eco24I GRGCYC 1 cut(s) 1359
Eco32I GATATC 2 cut(s) 1724, 1861
Eco47I GGWCC 2 cut(s) 2714, 3353
Eco52I CGGCCG 1 cut(s) 622
Eco53kI GAGCTC 1 cut(s) 1357
Eco57I CTGAAG 2 cut(s) 767, 3156
Eco88I CYCGRG 2 cut(s) 1264, 1297
Eco91I GGTNACC 1 cut(s) 1181
EcoICRI GAGCTC 1 cut(s) 1357
EcoO65I GGTNACC 1 cut(s) 1181
EcoRII CCWGG 2 cut(s) 2389, 3205
EcoRV GATATC 2 cut(s) 1724, 1861
EcoT22I ATGCAT 1 cut(s) 523
EcoT38I GRGCYC 1 cut(s) 1359
EgeI GGCGCC 2 cut(s) 136, 769
EheI GGCGCC 2 cut(s) 136, 769
Esp3I CGTCTC 1 cut(s) 2630
FaeI CATG 6 cut(s) 1363, 2749, 2804, 2888, 3316, 3334
FaqI GGGAC 7 cut(s) 164, 545, 809, 1475, 1619, 1856, 2367
FatI CATG 6 cut(s) 1359, 2745, 2800, 2884, 3312, 3330
FauI CCCGC 3 cut(s) 135, 238, 408
FauNDI CATATG 1 cut(s) 2482
FbaI TGATCA 1 cut(s) 27
FblI GTMKAC 3 cut(s) 250, 2322, 2736
FokI GGATG 2 cut(s) 17, 1259
FriOI GRGCYC 1 cut(s) 1359
FspBI CTAG 9 cut(s) 12, 884, 1011, 1304, 1380, 1875, 2847, 3200, 3221
GlaI GCGC 9 cut(s) 136, 185, 349, 408, 724, 738, 769, 897, 982
GsaI CCCAGC 2 cut(s) 151, 1496
GsuI CTGGAG 3 cut(s) 551, 614, 965
HaeII RGCGCY 4 cut(s) 138, 410, 726, 771
HaeIII GGCC 7 cut(s) 488, 624, 1197, 1990, 2449, 2815, 3401
HgaI GACGC 6 cut(s) 178, 1477, 2369, 2607, 2927, 3337
HhaI GCGC 9 cut(s) 137, 186, 350, 409, 725, 739, 770, 898, 983
Hin1I GRCGYC 4 cut(s) 135, 189, 768, 2599
Hin1II CATG 6 cut(s) 1363, 2749, 2804, 2888, 3316, 3334
Hin6I GCGC 9 cut(s) 135, 184, 348, 407, 723, 737, 768, 896, 981
HinP1I GCGC 9 cut(s) 135, 184, 348, 407, 723, 737, 768, 896, 981
HincII GTYRAC 4 cut(s) 71, 251, 856, 2964
HindII GTYRAC 4 cut(s) 71, 251, 856, 2964
HindIII AAGCTT 3 cut(s) 779, 2348, 2453
HpaI GTTAAC 1 cut(s) 2964
Hpy188III TCNNGA 7 cut(s) 631, 826, 944, 1169, 1175, 2141, 2717
Hpy99I CGWCG 6 cut(s) 194, 731, 1282, 2601, 3353, 3373
HpyCH4III ACNGT 4 cut(s) 364, 1731, 1741, 2692
HpyCH4IV ACGT 3 cut(s) 534, 1715, 2639
HpySE526I ACGT 3 cut(s) 534, 1715, 2639
Hsp92I GRCGYC 4 cut(s) 135, 189, 768, 2599
Hsp92II CATG 6 cut(s) 1363, 2749, 2804, 2888, 3316, 3334
HspAI GCGC 9 cut(s) 135, 184, 348, 407, 723, 737, 768, 896, 981
KasI GGCGCC 2 cut(s) 134, 767
KpnI GGTACC 2 cut(s) 502, 1272
KroI GCCGGC 3 cut(s) 403, 624, 1060
KroNI GCCGGC 3 cut(s) 405, 626, 1062
Ksp22I TGATCA 1 cut(s) 27
KspAI GTTAAC 1 cut(s) 2964
LguI GCTCTTC 1 cut(s) 1878
Lsp1109I GCAGC 7 cut(s) 574, 925, 1375, 1569, 2263, 3050, 3183
LweI GCATC 4 cut(s) 624, 2218, 2686, 2833
MaeI CTAG 9 cut(s) 12, 884, 1011, 1304, 1380, 1875, 2847, 3200, 3221
MaeII ACGT 3 cut(s) 534, 1715, 2639
MaeIII GTNAC 7 cut(s) 278, 1006, 1158, 1181, 1731, 2362, 3027
MbiI CCGCTC 3 cut(s) 130, 1122, 1323
MfeI CAATTG 2 cut(s) 1468, 3006
MflI RGATCY 5 cut(s) 87, 880, 2035, 2417, 3102
MhlI GDGCHC 4 cut(s) 1294, 1359, 2589, 2628
MlsI TGGCCA 1 cut(s) 2815
MluNI TGGCCA 1 cut(s) 2815
Mly113I GGCGCC 2 cut(s) 135, 768
MlyI GAGTC 5 cut(s) 146, 246, 650, 851, 3239
MmeI TCCRAC 7 cut(s) 720, 914, 1068, 3078, 3250, 3331, 3404
Mox20I TGGCCA 1 cut(s) 2815
Mph1103I ATGCAT 1 cut(s) 523
MreI CGCCGGCG 1 cut(s) 403
MroNI GCCGGC 3 cut(s) 403, 624, 1060
MscI TGGCCA 1 cut(s) 2815
MslI CAYNNNNRTG 3 cut(s) 35, 2793, 2889
Msp20I TGGCCA 1 cut(s) 2815
MspA1I CMGCKG 2 cut(s) 667, 2337
MspR9I CCNGG 8 cut(s) 215, 529, 1100, 2069, 2391, 3049, 3207, 3227
MunI CAATTG 2 cut(s) 1468, 3006
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 2 cut(s) 2391, 3207
MvnI CGCG 6 cut(s) 186, 245, 737, 983, 2427, 3348
NaeI GCCGGC 3 cut(s) 405, 626, 1062
NarI GGCGCC 2 cut(s) 135, 768
NciI CCSGG 6 cut(s) 215, 529, 1100, 2069, 3049, 3227
NdeI CATATG 1 cut(s) 2482
NgoMIV GCCGGC 3 cut(s) 403, 624, 1060
NlaIII CATG 6 cut(s) 1363, 2749, 2804, 2888, 3316, 3334
NlaIV GGNNCC 6 cut(s) 136, 322, 500, 629, 769, 1270
NmeAIII GCCGAG 3 cut(s) 111, 234, 1846
NmuCI GTSAC 4 cut(s) 278, 1006, 1731, 3027
NsiI ATGCAT 1 cut(s) 523
NspI RCATGY 1 cut(s) 2749
PciSI GCTCTTC 1 cut(s) 1878
PctI GAATGC 1 cut(s) 521
PdiI GCCGGC 3 cut(s) 405, 626, 1062
PfeI GAWTC 6 cut(s) 468, 1307, 1315, 1759, 2137, 3239
PflMI CCANNNNNTGG 1 cut(s) 1790
PfoI TCCNGGA 1 cut(s) 213
PinAI ACCGGT 1 cut(s) 2958
PleI GAGTC 5 cut(s) 146, 246, 650, 851, 3238
PluTI GGCGCC 2 cut(s) 138, 771
PpsI GAGTC 5 cut(s) 146, 246, 650, 851, 3238
Ppu21I YACGTR 1 cut(s) 1716
PshBI ATTAAT 1 cut(s) 2264
Psp124BI GAGCTC 1 cut(s) 1359
Psp6I CCWGG 2 cut(s) 2389, 3205
PspEI GGTNACC 1 cut(s) 1181
PspFI CCCAGC 2 cut(s) 147, 1492
PspGI CCWGG 2 cut(s) 2389, 3205
PspN4I GGNNCC 6 cut(s) 136, 322, 500, 629, 769, 1270
PspPI GGNCC 5 cut(s) 1195, 1988, 2447, 2714, 3353
PsuI RGATCY 5 cut(s) 87, 880, 2035, 2417, 3102
PvuII CAGCTG 1 cut(s) 667
RsaI GTAC 9 cut(s) 44, 500, 714, 1270, 1670, 1815, 2546, 2805, 3013
RsaNI GTAC 9 cut(s) 43, 499, 713, 1269, 1669, 1814, 2545, 2804, 3012
RseI CAYNNNNRTG 3 cut(s) 35, 2793, 2889
Rsr2I CGGWCCG 1 cut(s) 3353
RsrII CGGWCCG 1 cut(s) 3353
SacI GAGCTC 1 cut(s) 1359
SalI GTCGAC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 1878
Sau96I GGNCC 5 cut(s) 1195, 1988, 2447, 2714, 3353
ScaI AGTACT 1 cut(s) 1815
SchI GAGTC 5 cut(s) 146, 246, 650, 851, 3239
ScrFI CCNGG 8 cut(s) 215, 529, 1100, 2069, 2391, 3049, 3207, 3227
SduI GDGCHC 4 cut(s) 1294, 1359, 2589, 2628
SfaNI GCATC 4 cut(s) 624, 2218, 2686, 2833
SfcI CTRYAG 1 cut(s) 2323
SfoI GGCGCC 2 cut(s) 136, 769
SgrAI CRCCGGYG 2 cut(s) 403, 599
SinI GGWCC 2 cut(s) 2714, 3353
SmiMI CAYNNNNRTG 3 cut(s) 35, 2793, 2889
SmlI CTYRAG 4 cut(s) 638, 1638, 1817, 3215
SmoI CTYRAG 4 cut(s) 638, 1638, 1817, 3215
SspDI GGCGCC 2 cut(s) 134, 767
SspI AATATT 1 cut(s) 2077
SspMI CTAG 9 cut(s) 12, 884, 1011, 1304, 1380, 1875, 2847, 3200, 3221
SstI GAGCTC 1 cut(s) 1359
StyD4I CCNGG 8 cut(s) 213, 527, 1098, 2067, 2389, 3047, 3205, 3225
TaaI ACNGT 4 cut(s) 364, 1731, 1741, 2692
TaiI ACGT 3 cut(s) 537, 1718, 2642
TaqI TCGA 6 cut(s) 250, 659, 1026, 1280, 2596, 2628
TatI WGTACW 4 cut(s) 42, 712, 1813, 2803
TauI GCSGC 9 cut(s) 56, 189, 606, 919, 981, 2824, 2902, 3005, 3348
TfiI GAWTC 6 cut(s) 468, 1307, 1315, 1759, 2137, 3239
TscAI CASTG 4 cut(s) 873, 1533, 3048, 3129
TseFI GTSAC 4 cut(s) 278, 1006, 1731, 3027
TseI GCWGC 7 cut(s) 562, 913, 1363, 1582, 2276, 3038, 3196
Tsp45I GTSAC 4 cut(s) 278, 1006, 1731, 3027
TspDTI ATGAA 5 cut(s) 1947, 2198, 2556, 2873, 3319
TspGWI ACGGA 3 cut(s) 266, 1151, 2562
TspRI CASTG 4 cut(s) 873, 1533, 3048, 3129
Van91I CCANNNNNTGG 1 cut(s) 1790
VpaK11BI GGWCC 2 cut(s) 2714, 3353
VspI ATTAAT 1 cut(s) 2264
XapI RAATTY 8 cut(s) 1128, 1255, 1459, 1588, 1848, 1932, 2107, 2945
XceI RCATGY 1 cut(s) 2749
XmiI GTMKAC 3 cut(s) 250, 2322, 2736
XspI CTAG 9 cut(s) 12, 884, 1011, 1304, 1380, 1875, 2847, 3200, 3221
ZrmI AGTACT 1 cut(s) 1815
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.