Rroxscaffold_2G00138060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002289
Physical Location & Seq
Forward (+)
76101813 .. 76113980
12168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00138060.1

Sequence Viewer

Length: 552 bp
ATGCTCAACCCAGGCCTCCGCGACCTAGGCTTCGACGAATCAGATCGTCGAACTTCCGGTGCCACCGGAGGATCTCCACCGGTTGTGTACATGTCCGGTCCATCGGGTTGTCGGAGACCCGAACCGTCGGACAAACGCTCCTCCTCTCCGGCACGTCGGGTGCTATCGTCGTTCTCCTCCTCCTCGCCGTGGACGACGTTGATCCGCATCGAGCTTTCTCCAATGACCGTCGAAATAGGTCGTCAAGCTTCGGAGAAGCGGTCGACGTGCATCGCCGATGAGCTCCACCATCAACCTCGTCCCAAATCCAATCGATTCGGAGCTTCAAGGTCCGGCCATTTGCACCGCAATTTCACAGTTCCGCCGGCCGATCGGACAACAAATAGTGCGCGGTGCCTCCGTCCCTTGTCCTTCGCATCCGGTGCCCGAGCAATTTCTGGTTTACCAAACACCTCGAGTGCTTTTTTTCTCACAGCAAGTTTGGAAGTGTTTCCTCTCACAGCAAGTTCGGAACGAAACCTTAATGTAAATCGACTACTTAACTCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

19.81

Weight (kDa)

11.09

Isoelectric Point (pI)

76.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 59, 393, 422
AccI GTMKAC 1 cut(s) 263
AccII CGCG 2 cut(s) 21, 391
AciI CCGC 6 cut(s) 19, 205, 259, 346, 362, 391
AclWI GGATC 2 cut(s) 79, 196
AcoI YGGCCR 2 cut(s) 334, 366
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 1 cut(s) 189
AflIII ACRYGT 1 cut(s) 90
AgeI ACCGGT 1 cut(s) 79
AgsI TTSAA 1 cut(s) 327
AjiI CACGTC 2 cut(s) 155, 267
AjnI CCWGG 1 cut(s) 10
AluBI AGCT 4 cut(s) 214, 248, 283, 323
AluI AGCT 4 cut(s) 214, 248, 283, 323
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 1 cut(s) 109
AlwI GGATC 2 cut(s) 79, 196
Ama87I CYCGRG 2 cut(s) 426, 454
AoxI GGCC 3 cut(s) 13, 334, 366
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AsiGI ACCGGT 1 cut(s) 79
Asp700I GAANNNNTTC 1 cut(s) 489
AspA2I CCTAGG 1 cut(s) 25
AspLEI GCGC 1 cut(s) 391
AspS9I GGNCC 2 cut(s) 98, 330
AvaI CYCGRG 2 cut(s) 426, 454
AvaII GGWCC 2 cut(s) 98, 330
AvrII CCTAGG 1 cut(s) 25
BaeGI GKGCMC 1 cut(s) 427
BanI GGYRCC 3 cut(s) 59, 393, 422
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 285
BccI CCATC 2 cut(s) 109, 297
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 1 cut(s) 12
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 1 cut(s) 26
BlnI CCTAGG 1 cut(s) 25
Bme1390I CCNGG 1 cut(s) 12
Bme18I GGWCC 2 cut(s) 98, 330
BmeT110I CYCGRG 2 cut(s) 426, 454
BmgBI CACGTC 2 cut(s) 155, 267
BmgT120I GGNCC 2 cut(s) 98, 330
BmiI GGNNCC 3 cut(s) 61, 395, 424
BmrFI CCNGG 1 cut(s) 12
BmsI GCATC 3 cut(s) 216, 279, 425
Bsa29I ATCGAT 1 cut(s) 313
BsaBI GATNNNNATC 1 cut(s) 206
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 3 cut(s) 10, 25, 188
BsaWI WCCGGW 5 cut(s) 56, 65, 79, 95, 419
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 245, 275
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse118I RCCGGY 2 cut(s) 79, 364
Bse8I GATNNNNATC 1 cut(s) 206
BseBI CCWGG 1 cut(s) 12
BseCI ATCGAT 1 cut(s) 313
BseDI CCNNGG 3 cut(s) 10, 25, 188
BseGI GGATG 1 cut(s) 416
BseJI GATNNNNATC 1 cut(s) 206
BseLI CCNNNNNNNGG 1 cut(s) 189
BseRI GAGGAG 5 cut(s) 130, 133, 166, 169, 172
BseSI GKGCMC 1 cut(s) 427
BseX3I CGGCCG 1 cut(s) 366
Bsh1236I CGCG 2 cut(s) 21, 391
Bsh1285I CGRYCG 3 cut(s) 263, 369, 373
BshFI GGCC 3 cut(s) 15, 336, 368
BshNI GGYRCC 3 cut(s) 59, 393, 422
BshTI ACCGGT 1 cut(s) 79
BshVI ATCGAT 1 cut(s) 313
BsiEI CGRYCG 3 cut(s) 263, 369, 373
BsiHKAI GWGCWC 1 cut(s) 285
BsiHKCI CYCGRG 2 cut(s) 426, 454
BsiSI CCGG 8 cut(s) 57, 66, 80, 96, 149, 333, 365, 420
BslFI GGGAC 2 cut(s) 285, 387
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 1 cut(s) 109
BsmFI GGGAC 2 cut(s) 285, 387
BsnI GGCC 3 cut(s) 15, 336, 368
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 2 cut(s) 426, 454
Bsp1286I GDGCHC 2 cut(s) 285, 427
Bsp1407I TGTACA 1 cut(s) 87
Bsp143I GATC 4 cut(s) 43, 71, 201, 370
BspACI CCGC 6 cut(s) 19, 205, 259, 346, 362, 391
BspANI GGCC 3 cut(s) 15, 336, 368
BspDI ATCGAT 1 cut(s) 313
BspFNI CGCG 2 cut(s) 21, 391
BspLI GGNNCC 3 cut(s) 61, 395, 424
BspPI GGATC 2 cut(s) 79, 196
BspT107I GGYRCC 3 cut(s) 59, 393, 422
BspTNI GGTCTC 1 cut(s) 109
BsrFI RCCGGY 2 cut(s) 79, 364
BsrGI TGTACA 1 cut(s) 87
BssAI RCCGGY 2 cut(s) 79, 364
BssECI CCNNGG 3 cut(s) 10, 25, 188
BssMI GATC 4 cut(s) 43, 71, 201, 370
BssT1I CCWWGG 1 cut(s) 25
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 3 cut(s) 126, 229, 358
BstAPI GCANNNNNTGC 1 cut(s) 422
BstAUI TGTACA 1 cut(s) 87
BstC8I GCNNGC 1 cut(s) 366
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 416
BstFNI CGCG 2 cut(s) 21, 391
BstHHI GCGC 1 cut(s) 391
BstKTI GATC 4 cut(s) 46, 74, 204, 373
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 4 cut(s) 43, 71, 201, 370
BstMCI CGRYCG 3 cut(s) 263, 369, 373
BstMWI GCNNNNNNNGC 2 cut(s) 27, 422
BstNI CCWGG 1 cut(s) 12
BstNSI RCATGY 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 10
BstSLI GKGCMC 1 cut(s) 427
BstUI CGCG 2 cut(s) 21, 391
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
BstZI CGGCCG 1 cut(s) 366
Bsu15I ATCGAT 1 cut(s) 313
BsuRI GGCC 3 cut(s) 15, 336, 368
BsuTUI ATCGAT 1 cut(s) 313
BtgI CCRYGG 1 cut(s) 188
BtgZI GCGATG 1 cut(s) 256
BtrI CACGTC 2 cut(s) 155, 267
BtsCI GGATG 1 cut(s) 416
Cac8I GCNNGC 1 cut(s) 366
CfoI GCGC 1 cut(s) 391
Cfr10I RCCGGY 2 cut(s) 79, 364
Cfr13I GGNCC 2 cut(s) 98, 330
ClaI ATCGAT 1 cut(s) 313
Csp6I GTAC 1 cut(s) 88
CspAI ACCGGT 1 cut(s) 79
CviAII CATG 1 cut(s) 91
CviJI RGCY 8 cut(s) 15, 30, 214, 248, 283, 323, 336, 368
CviKI_1 RGCY 8 cut(s) 15, 30, 214, 248, 283, 323, 336, 368
CviQI GTAC 1 cut(s) 88
DpnI GATC 4 cut(s) 45, 73, 203, 372
DpnII GATC 4 cut(s) 43, 71, 201, 370
EaeI YGGCCR 2 cut(s) 334, 366
EagI CGGCCG 1 cut(s) 366
EciI GGCGGA 1 cut(s) 351
Ecl136II GAGCTC 1 cut(s) 283
EclXI CGGCCG 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 25
Eco147I AGGCCT 1 cut(s) 15
Eco24I GRGCYC 1 cut(s) 285
Eco31I GGTCTC 1 cut(s) 109
Eco47I GGWCC 2 cut(s) 98, 330
Eco52I CGGCCG 1 cut(s) 366
Eco53kI GAGCTC 1 cut(s) 283
Eco88I CYCGRG 2 cut(s) 426, 454
EcoICRI GAGCTC 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 25
FaeI CATG 1 cut(s) 94
FaiI YATR 1 cut(s) 92
FaqI GGGAC 2 cut(s) 285, 387
FatI CATG 1 cut(s) 90
FblI GTMKAC 1 cut(s) 263
FokI GGATG 1 cut(s) 403
FriOI GRGCYC 1 cut(s) 285
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 390
HaeIII GGCC 3 cut(s) 15, 336, 368
HapII CCGG 8 cut(s) 57, 66, 80, 96, 149, 333, 365, 420
HhaI GCGC 1 cut(s) 391
Hin1II CATG 1 cut(s) 94
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 2 cut(s) 38, 315
HpaII CCGG 8 cut(s) 57, 66, 80, 96, 149, 333, 365, 420
Hpy166II GTNNAC 4 cut(s) 88, 192, 264, 443
Hpy188I TCNGA 7 cut(s) 43, 114, 130, 253, 320, 375, 511
Hpy8I GTNNAC 4 cut(s) 88, 192, 264, 443
Hpy99I CGWCG 8 cut(s) 38, 51, 130, 159, 172, 199, 233, 268
HpyAV CCTTC 1 cut(s) 421
HpyCH4III ACNGT 3 cut(s) 126, 229, 358
HpyCH4IV ACGT 3 cut(s) 154, 197, 266
HpyCH4V TGCA 2 cut(s) 270, 343
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 422
HpySE526I ACGT 3 cut(s) 154, 197, 266
Hsp92II CATG 1 cut(s) 94
HspAI GCGC 1 cut(s) 389
KroI GCCGGC 1 cut(s) 364
KroNI GCCGGC 1 cut(s) 366
Kzo9I GATC 4 cut(s) 43, 71, 201, 370
LmnI GCTCC 3 cut(s) 143, 288, 320
LweI GCATC 3 cut(s) 216, 279, 425
MaeI CTAG 1 cut(s) 26
MaeII ACGT 3 cut(s) 154, 197, 266
MalI GATC 4 cut(s) 45, 73, 203, 372
MboI GATC 4 cut(s) 43, 71, 201, 370
MboII GAAGA 1 cut(s) 537
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 2 cut(s) 285, 427
MluCI AATT 2 cut(s) 349, 432
MmeI TCCRAC 2 cut(s) 92, 108
MroNI GCCGGC 1 cut(s) 364
MroXI GAANNNNTTC 1 cut(s) 489
MseI TTAA 3 cut(s) 522, 540, 550
MspI CCGG 8 cut(s) 57, 66, 80, 96, 149, 333, 365, 420
MspR9I CCNGG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 12
MvnI CGCG 2 cut(s) 21, 391
MwoI GCNNNNNNNGC 2 cut(s) 27, 422
NaeI GCCGGC 1 cut(s) 366
NdeII GATC 4 cut(s) 43, 71, 201, 370
NgoMIV GCCGGC 1 cut(s) 364
NlaIII CATG 1 cut(s) 94
NlaIV GGNNCC 3 cut(s) 61, 395, 424
NspI RCATGY 1 cut(s) 94
PaeR7I CTCGAG 1 cut(s) 454
PceI AGGCCT 1 cut(s) 15
PciI ACATGT 1 cut(s) 90
PcsI WCGNNNNNNNCGW 1 cut(s) 191
PdiI GCCGGC 1 cut(s) 366
PdmI GAANNNNTTC 1 cut(s) 489
PfeI GAWTC 2 cut(s) 38, 315
PinAI ACCGGT 1 cut(s) 79
Ple19I CGATCG 1 cut(s) 373
PscI ACATGT 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 3 cut(s) 61, 395, 424
PspPI GGNCC 2 cut(s) 98, 330
PspXI VCTCGAGB 1 cut(s) 454
PsuI RGATCY 1 cut(s) 71
PvuI CGATCG 1 cut(s) 373
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SacI GAGCTC 1 cut(s) 285
SalI GTCGAC 1 cut(s) 262
SaqAI TTAA 3 cut(s) 522, 540, 550
Sau3AI GATC 4 cut(s) 43, 71, 201, 370
Sau96I GGNCC 2 cut(s) 98, 330
ScrFI CCNGG 1 cut(s) 12
SduI GDGCHC 2 cut(s) 285, 427
SfaNI GCATC 3 cut(s) 216, 279, 425
Sfr274I CTCGAG 1 cut(s) 454
SinI GGWCC 2 cut(s) 98, 330
SlaI CTCGAG 1 cut(s) 454
SmlI CTYRAG 1 cut(s) 454
SmoI CTYRAG 1 cut(s) 454
Sse9I AATT 2 cut(s) 349, 432
SseBI AGGCCT 1 cut(s) 15
SsiI CCGC 6 cut(s) 19, 205, 259, 346, 362, 391
SspMI CTAG 1 cut(s) 26
SstI GAGCTC 1 cut(s) 285
StuI AGGCCT 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 10
StyI CCWWGG 1 cut(s) 25
TaaI ACNGT 3 cut(s) 126, 229, 358
TaiI ACGT 3 cut(s) 157, 200, 269
TaqI TCGA 8 cut(s) 33, 49, 210, 231, 263, 313, 455, 532
TasI AATT 2 cut(s) 349, 432
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 2 cut(s) 38, 315
Tru1I TTAA 3 cut(s) 522, 540, 550
Tru9I TTAA 3 cut(s) 522, 540, 550
TspGWI ACGGA 1 cut(s) 389
VpaK11BI GGWCC 2 cut(s) 98, 330
XceI RCATGY 1 cut(s) 94
XhoI CTCGAG 1 cut(s) 454
XmaJI CCTAGG 1 cut(s) 25
XmiI GTMKAC 1 cut(s) 263
XmnI GAANNNNTTC 1 cut(s) 489
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.