Rmu_sc0016328.1_g000001

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016328.1
OG0002289
Physical Location & Seq
Reverse (-)
1 .. 715
715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016328.1_g000001.1.cds

Sequence Viewer

Length: 414 bp
atggcttcttcttcaattcactacgatattcgctgcgcccttcttcttcacttggccacgtcatcttcctcatcgccgtcgtcgtcgtcgtcgctatccacgattaggactctcggtccccaccgcataacctggcgcgcctccgcggcgcgtcgggtgctgtcgtcgttctcctcatcgccgcggacgacgtcgttccgcgtcagagctttctccaatgacgccgtggagcttccggagaagccgtcgatatgcaccgccgacgagctccactacgtctccgtgcccgactccgattggaggctcgccctctggcgctaccgcccttgccccaaggcccctcagaggaatcacccattgctgctattgtctggcgtggggaccaatgcaattggatacgatctctctcctgag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

138

Amino Acids

15.15

Weight (kDa)

9.56

Isoelectric Point (pI)

64.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 194
AccII CGCG 5 cut(s) 138, 146, 151, 184, 201
AccIII TCCGGA 1 cut(s) 235
AciI CCGC 8 cut(s) 124, 144, 146, 182, 184, 199, 258, 322
AcoI YGGCCR 1 cut(s) 54
AcyI GRCGYC 2 cut(s) 191, 222
AfiI CCNNNNNNNGG 3 cut(s) 105, 300, 345
AgsI TTSAA 1 cut(s) 15
AhdI GACNNNNNGTC 1 cut(s) 114
AjiI CACGTC 1 cut(s) 60
AjnI CCWGG 1 cut(s) 131
AluBI AGCT 3 cut(s) 209, 232, 268
AluI AGCT 3 cut(s) 209, 232, 268
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 283
Aor13HI TCCGGA 1 cut(s) 235
AoxI GGCC 2 cut(s) 54, 336
ApeKI GCWGC 2 cut(s) 33, 361
AscI GGCGCGCC 1 cut(s) 136
AspLEI GCGC 5 cut(s) 38, 138, 140, 151, 318
AspS9I GGNCC 3 cut(s) 116, 337, 381
AsuHPI GGTGA 1 cut(s) 344
AvaII GGWCC 2 cut(s) 116, 381
BaeGI GKGCMC 1 cut(s) 288
BalI TGGCCA 1 cut(s) 56
BanII GRGCYC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 2 cut(s) 20, 348
BceAI ACGGC 3 cut(s) 61, 209, 229
BciT130I CCWGG 1 cut(s) 133
BciVI GTATCC 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 283
BfoI RGCGCY 1 cut(s) 319
BfuI GTATCC 1 cut(s) 389
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 4 cut(s) 34, 147, 182, 362
BlsI GCNGC 4 cut(s) 35, 148, 183, 363
Bme1390I CCNGG 1 cut(s) 133
Bme18I GGWCC 2 cut(s) 116, 381
BmeRI GACNNNNNGTC 1 cut(s) 114
BmgBI CACGTC 1 cut(s) 60
BmgT120I GGNCC 3 cut(s) 116, 337, 381
BmiI GGNNCC 3 cut(s) 118, 339, 382
BmrFI CCNGG 1 cut(s) 133
BsaHI GRCGYC 2 cut(s) 191, 222
BsaJI CCNNGG 4 cut(s) 144, 182, 225, 333
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 4 cut(s) 230, 260, 263, 293
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 300, 345
Bse3DI GCAATG 1 cut(s) 356
BseAI TCCGGA 1 cut(s) 235
BseBI CCWGG 1 cut(s) 133
BseDI CCNNGG 4 cut(s) 144, 182, 225, 333
BseLI CCNNNNNNNGG 3 cut(s) 105, 300, 345
BseMI GCAATG 1 cut(s) 356
BseMII CTCAG 2 cut(s) 356, 402
BsePI GCGCGC 1 cut(s) 136
BseRI GAGGAG 1 cut(s) 163
BseSI GKGCMC 1 cut(s) 288
BseXI GCAGC 2 cut(s) 20, 348
Bsh1236I CGCG 5 cut(s) 138, 146, 151, 184, 201
BshFI GGCC 2 cut(s) 56, 338
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 1 cut(s) 236
BslFI GGGAC 2 cut(s) 102, 394
BslI CCNNNNNNNGG 3 cut(s) 105, 300, 345
BsmAI GTCTC 1 cut(s) 283
BsmBI CGTCTC 1 cut(s) 283
BsmFI GGGAC 2 cut(s) 102, 394
BsnI GGCC 2 cut(s) 56, 338
Bsp1286I GDGCHC 2 cut(s) 270, 288
Bsp13I TCCGGA 1 cut(s) 235
Bsp143I GATC 1 cut(s) 400
BspACI CCGC 8 cut(s) 124, 144, 146, 182, 184, 199, 258, 322
BspANI GGCC 2 cut(s) 56, 338
BspCNI CTCAG 2 cut(s) 355, 403
BspEI TCCGGA 1 cut(s) 235
BspFNI CGCG 5 cut(s) 138, 146, 151, 184, 201
BspLI GGNNCC 3 cut(s) 118, 339, 382
BsrDI GCAATG 1 cut(s) 356
BssECI CCNNGG 4 cut(s) 144, 182, 225, 333
BssHII GCGCGC 1 cut(s) 136
BssMI GATC 1 cut(s) 400
BssNI GRCGYC 2 cut(s) 191, 222
BssT1I CCWWGG 1 cut(s) 333
Bst2UI CCWGG 1 cut(s) 133
BstACI GRCGYC 2 cut(s) 191, 222
BstC8I GCNNGC 2 cut(s) 138, 306
BstDEI CTNAG 2 cut(s) 342, 411
BstDSI CCRYGG 3 cut(s) 144, 182, 225
BstFNI CGCG 5 cut(s) 138, 146, 151, 184, 201
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 5 cut(s) 38, 138, 140, 151, 318
BstKTI GATC 1 cut(s) 403
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 1 cut(s) 400
BstMWI GCNNNNNNNGC 2 cut(s) 146, 157
BstNI CCWGG 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 131
BstSLI GKGCMC 1 cut(s) 288
BstUI CGCG 5 cut(s) 138, 146, 151, 184, 201
BstV1I GCAGC 2 cut(s) 20, 348
BsuI GTATCC 1 cut(s) 389
BsuRI GGCC 2 cut(s) 56, 338
BtgI CCRYGG 3 cut(s) 144, 182, 225
BtgZI GCGATG 2 cut(s) 57, 162
BtrI CACGTC 1 cut(s) 60
Cac8I GCNNGC 2 cut(s) 138, 306
CfoI GCGC 5 cut(s) 38, 138, 140, 151, 318
Cfr13I GGNCC 3 cut(s) 116, 337, 381
Cfr42I CCGCGG 2 cut(s) 147, 185
CseI GACGC 3 cut(s) 140, 190, 230
CviJI RGCY 8 cut(s) 5, 56, 209, 232, 244, 268, 304, 338
CviKI_1 RGCY 8 cut(s) 5, 56, 209, 232, 244, 268, 304, 338
DdeI CTNAG 2 cut(s) 342, 411
DpnI GATC 1 cut(s) 402
DpnII GATC 1 cut(s) 400
DriI GACNNNNNGTC 1 cut(s) 114
EaeI YGGCCR 1 cut(s) 54
Eam1105I GACNNNNNGTC 1 cut(s) 114
Ecl136II GAGCTC 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 333
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 2 cut(s) 116, 381
Eco53kI GAGCTC 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 268
EcoO109I RGGNCCY 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 333
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 333
Esp3I CGTCTC 1 cut(s) 283
FaiI YATR 2 cut(s) 128, 253
FaqI GGGAC 2 cut(s) 102, 394
Fnu4HI GCNGC 4 cut(s) 34, 147, 182, 362
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 4 cut(s) 34, 147, 182, 362
GlaI GCGC 5 cut(s) 37, 137, 139, 150, 317
GluI GCNGC 4 cut(s) 34, 147, 182, 362
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 2 cut(s) 56, 338
HapII CCGG 1 cut(s) 236
HgaI GACGC 3 cut(s) 140, 190, 230
HhaI GCGC 5 cut(s) 38, 138, 140, 151, 318
Hin1I GRCGYC 2 cut(s) 191, 222
Hin6I GCGC 5 cut(s) 36, 136, 138, 149, 316
HinP1I GCGC 5 cut(s) 36, 136, 138, 149, 316
HinfI GANTC 3 cut(s) 109, 290, 349
HpaII CCGG 1 cut(s) 236
HphI GGTGA 1 cut(s) 344
Hpy188I TCNGA 3 cut(s) 206, 295, 345
Hpy188III TCNNGA 2 cut(s) 236, 410
HpyAV CCTTC 1 cut(s) 50
HpyCH4IV ACGT 3 cut(s) 59, 191, 276
HpyCH4V TGCA 2 cut(s) 255, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 157
HpyF3I CTNAG 2 cut(s) 342, 411
HpySE526I ACGT 3 cut(s) 59, 191, 276
Hsp92I GRCGYC 2 cut(s) 191, 222
HspAI GCGC 5 cut(s) 36, 136, 138, 149, 316
Kpn2I TCCGGA 1 cut(s) 235
KspI CCGCGG 2 cut(s) 147, 185
Kzo9I GATC 1 cut(s) 400
LmnI GCTCC 2 cut(s) 229, 273
LpnPI CCDG 5 cut(s) 118, 145, 249, 298, 357
Lsp1109I GCAGC 2 cut(s) 20, 348
MaeII ACGT 3 cut(s) 59, 191, 276
MalI GATC 1 cut(s) 402
MboI GATC 1 cut(s) 400
MboII GAAGA 4 cut(s) 3, 35, 38, 57
MfeI CAATTG 1 cut(s) 390
MhlI GDGCHC 2 cut(s) 270, 288
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 2 cut(s) 15, 390
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 2 cut(s) 103, 284
MnlI CCTC 7 cut(s) 79, 151, 184, 294, 320, 339, 351
Mox20I TGGCCA 1 cut(s) 56
MroI TCCGGA 1 cut(s) 235
MscI TGGCCA 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 2 cut(s) 146, 184
MspI CCGG 1 cut(s) 236
MspR9I CCNGG 1 cut(s) 133
MunI CAATTG 1 cut(s) 390
MvaI CCWGG 1 cut(s) 133
MvnI CGCG 5 cut(s) 138, 146, 151, 184, 201
MwoI GCNNNNNNNGC 2 cut(s) 146, 157
NdeII GATC 1 cut(s) 400
NlaIV GGNNCC 3 cut(s) 118, 339, 382
PalAI GGCGCGCC 1 cut(s) 136
PauI GCGCGC 1 cut(s) 136
PcsI WCGNNNNNNNCGW 4 cut(s) 80, 86, 98, 185
PfeI GAWTC 1 cut(s) 349
PflFI GACNNNGTC 1 cut(s) 190
PkrI GCNGC 4 cut(s) 35, 148, 183, 363
PleI GAGTC 2 cut(s) 103, 284
PpsI GAGTC 2 cut(s) 103, 284
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
PspN4I GGNNCC 3 cut(s) 118, 339, 382
PspPI GGNCC 3 cut(s) 116, 337, 381
PsyI GACNNNGTC 1 cut(s) 190
PteI GCGCGC 1 cut(s) 136
SacI GAGCTC 1 cut(s) 270
SacII CCGCGG 2 cut(s) 147, 185
SatI GCNGC 4 cut(s) 34, 147, 182, 362
Sau3AI GATC 1 cut(s) 400
Sau96I GGNCC 3 cut(s) 116, 337, 381
SchI GAGTC 2 cut(s) 103, 284
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 2 cut(s) 270, 288
SetI ASST 7 cut(s) 62, 134, 194, 211, 234, 270, 279
Sfr303I CCGCGG 2 cut(s) 147, 185
SgrBI CCGCGG 2 cut(s) 147, 185
SgsI GGCGCGCC 1 cut(s) 136
SinI GGWCC 2 cut(s) 116, 381
Sse9I AATT 2 cut(s) 15, 390
SsiI CCGC 8 cut(s) 124, 144, 146, 182, 184, 199, 258, 322
SstI GAGCTC 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 131
StyI CCWWGG 1 cut(s) 333
TaiI ACGT 3 cut(s) 62, 194, 279
TaqI TCGA 1 cut(s) 248
TaqII GACCGA 1 cut(s) 104
TasI AATT 2 cut(s) 15, 390
TauI GCSGC 2 cut(s) 149, 184
TfiI GAWTC 1 cut(s) 349
TseI GCWGC 2 cut(s) 33, 361
TspGWI ACGGA 1 cut(s) 271
Tth111I GACNNNGTC 1 cut(s) 190
VpaK11BI GGWCC 2 cut(s) 116, 381
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
ZraI GACGTC 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.