Rmu_sc0005157.1_g000016

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005157.1
OG0002289
Physical Location & Seq
Forward (+)
52820 .. 53236
417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005157.1_g000016.1.cds

Sequence Viewer

Length: 417 bp
atggcttcttcttcaatccactacgatattcgctgcacccttcttcttcacttggccacgtcatcttcatcgacatcatcgtcaccttcgctgtcatcgtcgtcgtccacgattaggactctcagtccccaccgcataacctcgcgcgcctcctcggcacgtcgggtgctgtcgtcgttctcctcctcgccgtggacgacgtcgatccgcatcagagctttctccaatgaccagtcgaaagaggccgtcaagcttctggagaagcagtcaacatgcaccgccgatgagctccaccatcaacctcctcccaaatctaatcgattctggagcttcaaggtccggccatttgcacctgcaatttcacagttccggccgatcagacaacaaatcgccggtgcctccatccctggtccataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

138

Amino Acids

15.11

Weight (kDa)

11.36

Isoelectric Point (pI)

76.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 361
AasI GACNNNNNNGTC 1 cut(s) 79
AatII GACGTC 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 361
AccB1I GGYRCC 1 cut(s) 395
AccII CGCG 2 cut(s) 145, 147
AciI CCGC 3 cut(s) 133, 208, 279
AclWI GGATC 1 cut(s) 199
AcoI YGGCCR 3 cut(s) 54, 341, 371
AcyI GRCGYC 1 cut(s) 200
AfiI CCNNNNNNNGG 2 cut(s) 114, 192
AgsI TTSAA 2 cut(s) 15, 334
AhdI GACNNNNNGTC 1 cut(s) 123
AjiI CACGTC 2 cut(s) 60, 161
AjnI CCWGG 1 cut(s) 406
AluBI AGCT 4 cut(s) 218, 253, 289, 330
AluI AGCT 4 cut(s) 218, 253, 289, 330
Alw21I GWGCWC 1 cut(s) 291
AlwI GGATC 1 cut(s) 199
AoxI GGCC 4 cut(s) 54, 243, 341, 371
ApeKI GCWGC 1 cut(s) 33
AspLEI GCGC 2 cut(s) 147, 149
AspS9I GGNCC 2 cut(s) 337, 410
AsuHPI GGTGA 1 cut(s) 75
AvaII GGWCC 2 cut(s) 337, 410
BalI TGGCCA 1 cut(s) 56
BanI GGYRCC 1 cut(s) 395
BanII GRGCYC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 1 cut(s) 20
BccI CCATC 2 cut(s) 303, 410
BceAI ACGGC 2 cut(s) 175, 230
BciT130I CCWGG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 361
BglI GCCNNNNNGGC 1 cut(s) 155
BisI GCNGC 1 cut(s) 34
BlsI GCNGC 1 cut(s) 35
Bme1390I CCNGG 1 cut(s) 408
Bme18I GGWCC 2 cut(s) 337, 410
BmeRI GACNNNNNGTC 1 cut(s) 123
BmgBI CACGTC 2 cut(s) 60, 161
BmgT120I GGNCC 2 cut(s) 337, 410
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 1 cut(s) 408
BmsI GCATC 1 cut(s) 219
BpmI CTGGAG 2 cut(s) 278, 346
Bsa29I ATCGAT 1 cut(s) 319
BsaBI GATNNNNATC 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 200
BsaJI CCNNGG 3 cut(s) 153, 191, 406
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 192
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 1 cut(s) 232
Bse8I GATNNNNATC 1 cut(s) 209
BseBI CCWGG 1 cut(s) 408
BseCI ATCGAT 1 cut(s) 319
BseDI CCNNGG 3 cut(s) 153, 191, 406
BseGI GGATG 1 cut(s) 402
BseJI GATNNNNATC 1 cut(s) 209
BseLI CCNNNNNNNGG 2 cut(s) 114, 192
BseMII CTCAG 1 cut(s) 136
BseNI ACTGG 1 cut(s) 232
BsePI GCGCGC 1 cut(s) 145
BseRI GAGGAG 4 cut(s) 142, 172, 175, 294
BseX3I CGGCCG 1 cut(s) 371
BseXI GCAGC 1 cut(s) 20
BsgI GTGCAG 1 cut(s) 19
Bsh1236I CGCG 2 cut(s) 145, 147
Bsh1285I CGRYCG 1 cut(s) 374
BshFI GGCC 4 cut(s) 56, 245, 343, 373
BshNI GGYRCC 1 cut(s) 395
BshVI ATCGAT 1 cut(s) 319
BsiEI CGRYCG 1 cut(s) 374
BsiHKAI GWGCWC 1 cut(s) 291
BsiSI CCGG 3 cut(s) 340, 370, 393
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 2 cut(s) 114, 192
BsmFI GGGAC 1 cut(s) 111
BsnI GGCC 4 cut(s) 56, 245, 343, 373
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 2 cut(s) 204, 375
BspACI CCGC 3 cut(s) 133, 208, 279
BspANI GGCC 4 cut(s) 56, 245, 343, 373
BspCNI CTCAG 1 cut(s) 135
BspDI ATCGAT 1 cut(s) 319
BspFNI CGCG 2 cut(s) 145, 147
BspLI GGNNCC 1 cut(s) 397
BspMI ACCTGC 1 cut(s) 361
BspPI GGATC 1 cut(s) 199
BspT107I GGYRCC 1 cut(s) 395
BsrFI RCCGGY 1 cut(s) 392
BsrI ACTGG 1 cut(s) 232
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 3 cut(s) 153, 191, 406
BssHII GCGCGC 1 cut(s) 145
BssMI GATC 2 cut(s) 204, 375
BssNI GRCGYC 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 1 cut(s) 366
BstACI GRCGYC 1 cut(s) 200
BstC8I GCNNGC 1 cut(s) 147
BstDEI CTNAG 1 cut(s) 122
BstDSI CCRYGG 1 cut(s) 191
BstF5I GGATG 1 cut(s) 402
BstFNI CGCG 2 cut(s) 145, 147
BstHHI GCGC 2 cut(s) 147, 149
BstKTI GATC 2 cut(s) 207, 378
BstMBI GATC 2 cut(s) 204, 375
BstMCI CGRYCG 1 cut(s) 374
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 1 cut(s) 408
BstNSI RCATGY 1 cut(s) 276
BstSCI CCNGG 1 cut(s) 406
BstUI CGCG 2 cut(s) 145, 147
BstV1I GCAGC 1 cut(s) 20
BstZI CGGCCG 1 cut(s) 371
Bsu15I ATCGAT 1 cut(s) 319
BsuRI GGCC 4 cut(s) 56, 245, 343, 373
BsuTUI ATCGAT 1 cut(s) 319
BtgI CCRYGG 1 cut(s) 191
BtrI CACGTC 2 cut(s) 60, 161
BtsCI GGATG 1 cut(s) 402
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 1 cut(s) 147
CfoI GCGC 2 cut(s) 147, 149
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 2 cut(s) 337, 410
ClaI ATCGAT 1 cut(s) 319
CviAII CATG 1 cut(s) 273
CviJI RGCY 9 cut(s) 5, 56, 218, 245, 253, 289, 330, 343, 373
CviKI_1 RGCY 9 cut(s) 5, 56, 218, 245, 253, 289, 330, 343, 373
DdeI CTNAG 1 cut(s) 122
DpnI GATC 2 cut(s) 206, 377
DpnII GATC 2 cut(s) 204, 375
DrdI GACNNNNNNGTC 1 cut(s) 79
DriI GACNNNNNGTC 1 cut(s) 123
DseDI GACNNNNNNGTC 1 cut(s) 79
EaeI YGGCCR 3 cut(s) 54, 341, 371
EagI CGGCCG 1 cut(s) 371
Eam1105I GACNNNNNGTC 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 289
EclXI CGGCCG 1 cut(s) 371
Eco24I GRGCYC 1 cut(s) 291
Eco47I GGWCC 2 cut(s) 337, 410
Eco52I CGGCCG 1 cut(s) 371
Eco53kI GAGCTC 1 cut(s) 289
EcoICRI GAGCTC 1 cut(s) 289
EcoRII CCWGG 1 cut(s) 406
EcoT38I GRGCYC 1 cut(s) 291
FaeI CATG 1 cut(s) 276
FaiI YATR 3 cut(s) 137, 274, 415
FaqI GGGAC 1 cut(s) 111
FatI CATG 1 cut(s) 272
Fnu4HI GCNGC 1 cut(s) 34
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 1 cut(s) 34
GlaI GCGC 2 cut(s) 146, 148
GluI GCNGC 1 cut(s) 34
GsuI CTGGAG 2 cut(s) 278, 346
HaeIII GGCC 4 cut(s) 56, 245, 343, 373
HapII CCGG 3 cut(s) 340, 370, 393
HhaI GCGC 2 cut(s) 147, 149
Hin1I GRCGYC 1 cut(s) 200
Hin1II CATG 1 cut(s) 276
Hin6I GCGC 2 cut(s) 145, 147
HinP1I GCGC 2 cut(s) 145, 147
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 2 cut(s) 118, 321
HpaII CCGG 3 cut(s) 340, 370, 393
HphI GGTGA 1 cut(s) 75
Hpy166II GTNNAC 3 cut(s) 108, 195, 270
Hpy188I TCNGA 2 cut(s) 215, 380
Hpy188III TCNNGA 2 cut(s) 257, 325
Hpy8I GTNNAC 3 cut(s) 108, 195, 270
Hpy99I CGWCG 6 cut(s) 103, 106, 165, 178, 202, 205
HpyAV CCTTC 2 cut(s) 50, 96
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4IV ACGT 3 cut(s) 59, 160, 200
HpyCH4V TGCA 4 cut(s) 36, 276, 350, 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 3 cut(s) 59, 160, 200
Hsp92I GRCGYC 1 cut(s) 200
Hsp92II CATG 1 cut(s) 276
HspAI GCGC 2 cut(s) 145, 147
Kzo9I GATC 2 cut(s) 204, 375
LmnI GCTCC 2 cut(s) 294, 327
LpnPI CCDG 8 cut(s) 242, 245, 310, 353, 366, 383, 393, 406
Lsp1109I GCAGC 1 cut(s) 20
LweI GCATC 1 cut(s) 219
MaeII ACGT 3 cut(s) 59, 160, 200
MaeIII GTNAC 1 cut(s) 81
MalI GATC 2 cut(s) 206, 377
MboI GATC 2 cut(s) 204, 375
MboII GAAGA 4 cut(s) 3, 35, 38, 57
MhlI GDGCHC 1 cut(s) 291
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 1 cut(s) 357
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 1 cut(s) 112
MnlI CCTC 9 cut(s) 151, 160, 163, 193, 196, 235, 312, 315, 409
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 56
MspI CCGG 3 cut(s) 340, 370, 393
MspR9I CCNGG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 408
MvnI CGCG 2 cut(s) 145, 147
MwoI GCNNNNNNNGC 1 cut(s) 155
NdeII GATC 2 cut(s) 204, 375
NlaIII CATG 1 cut(s) 276
NlaIV GGNNCC 1 cut(s) 397
NmeAIII GCCGAG 1 cut(s) 134
NmuCI GTSAC 1 cut(s) 81
NspI RCATGY 1 cut(s) 276
PaqCI CACCTGC 1 cut(s) 361
PauI GCGCGC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 4 cut(s) 77, 95, 107, 194
PfeI GAWTC 1 cut(s) 321
PflFI GACNNNGTC 1 cut(s) 199
PkrI GCNGC 1 cut(s) 35
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 1 cut(s) 397
PspPI GGNCC 2 cut(s) 337, 410
PsyI GACNNNGTC 1 cut(s) 199
PteI GCGCGC 1 cut(s) 145
SacI GAGCTC 1 cut(s) 291
SatI GCNGC 1 cut(s) 34
Sau3AI GATC 2 cut(s) 204, 375
Sau96I GGNCC 2 cut(s) 337, 410
SchI GAGTC 1 cut(s) 112
ScrFI CCNGG 1 cut(s) 408
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 1 cut(s) 219
SgrAI CRCCGGYG 1 cut(s) 392
SinI GGWCC 2 cut(s) 337, 410
Sse9I AATT 1 cut(s) 357
SsiI CCGC 3 cut(s) 133, 208, 279
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 406
TaaI ACNGT 1 cut(s) 366
TaiI ACGT 3 cut(s) 62, 163, 203
TaqI TCGA 4 cut(s) 71, 203, 236, 319
TasI AATT 1 cut(s) 357
TfiI GAWTC 1 cut(s) 321
TseFI GTSAC 1 cut(s) 81
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 1 cut(s) 81
TspDTI ATGAA 1 cut(s) 57
Tth111I GACNNNGTC 1 cut(s) 199
VpaK11BI GGWCC 2 cut(s) 337, 410
XceI RCATGY 1 cut(s) 276
ZraI GACGTC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.