Rmu_sc0001986.1_g000009

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001986.1
OG0002289
Physical Location & Seq
Reverse (-)
42337 .. 44663
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001986.1_g000009.1.cds

Sequence Viewer

Length: 756 bp
atggcttcttcttcaatccactacgatattcgctacacccttcttcttcacttggccacgtcatcttcatcgacatcatcgtcatcttcgccgtcatcgtcgtcgtccacgattaggactctcagtccccaccgcataacctcgcgcgcctcctcggcacgtcgggtgctgtcgtcattctcctcctcgccgtggatgacgtcgatccgcatcagagctttctccaatgaccagtcgaaagaggccgtcaagcttctggagaagcagtcgacatgcaccgccgatgagctccaccatcaacctcctcccaaatctaatcgattctggagcttcaaggtctggccatttgcacctgcaatttcacagttccggccgatcaaacagcaaatcgccggtgcctccacgccggaatcccgacaccagtccgatagccggattcctgattccggtcaatcgattgtcggattccaatcaccggtcaccggtcgcctgatttcggtcaccggtctccggattccggtcactagtcgccggagtccgctgccggccactacggagatcggtcttatcgaagccgatgatgacttcctcgatgaagtagcgagtcggactgaagtgacctctctcccggagcagcagcaacgacttagtgtcgtcgatgaagccgagaacgaaggcgaaaatcgctgttacagtctggtaccctctggcatcggcggacctcaattcagaccaaatattggcctcacggtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

251

Amino Acids

27.43

Weight (kDa)

9.06

Isoelectric Point (pI)

77.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 361
AasI GACNNNNNNGTC 1 cut(s) 79
AatII GACGTC 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 361
Acc65I GGTACC 1 cut(s) 700
AccB1I GGYRCC 2 cut(s) 395, 700
AccB7I CCANNNNNTGG 1 cut(s) 740
AccI GTMKAC 1 cut(s) 269
AccII CGCG 2 cut(s) 145, 147
AccIII TCCGGA 1 cut(s) 510
AciI CCGC 5 cut(s) 133, 208, 279, 539, 717
AclWI GGATC 1 cut(s) 199
AcoI YGGCCR 4 cut(s) 54, 341, 371, 546
AcuI CTGAAG 1 cut(s) 633
AcyI GRCGYC 1 cut(s) 200
AfaI GTAC 1 cut(s) 702
AgeI ACCGGT 3 cut(s) 475, 482, 503
AgsI TTSAA 2 cut(s) 15, 334
AhdI GACNNNNNGTC 2 cut(s) 123, 650
AhlI ACTAGT 1 cut(s) 524
AjiI CACGTC 2 cut(s) 60, 161
AluBI AGCT 4 cut(s) 218, 253, 289, 330
AluI AGCT 4 cut(s) 218, 253, 289, 330
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 1 cut(s) 512
AlwI GGATC 1 cut(s) 199
Aor13HI TCCGGA 1 cut(s) 510
AoxI GGCC 6 cut(s) 54, 243, 341, 371, 546, 742
ApeKI GCWGC 3 cut(s) 541, 634, 637
AsiGI ACCGGT 3 cut(s) 475, 482, 503
Asp718I GGTACC 1 cut(s) 700
AspLEI GCGC 2 cut(s) 147, 149
AspS9I GGNCC 1 cut(s) 719
AsuC2I CCSGG 1 cut(s) 629
AsuHPI GGTGA 3 cut(s) 465, 472, 493
AvaII GGWCC 1 cut(s) 719
BalI TGGCCA 2 cut(s) 56, 343
BanI GGYRCC 2 cut(s) 395, 700
BanII GRGCYC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 3 cut(s) 528, 646, 649
BccI CCATC 1 cut(s) 303
BceAI ACGGC 3 cut(s) 76, 175, 230
BcnI CCSGG 1 cut(s) 629
BcoDI GTCTC 1 cut(s) 512
BcuI ACTAGT 1 cut(s) 524
BfaI CTAG 1 cut(s) 525
BfuAI ACCTGC 1 cut(s) 361
BglI GCCNNNNNGGC 1 cut(s) 155
BisI GCNGC 3 cut(s) 542, 635, 638
BlsI GCNGC 3 cut(s) 543, 636, 639
Bme1390I CCNGG 1 cut(s) 629
Bme18I GGWCC 1 cut(s) 719
BmeRI GACNNNNNGTC 2 cut(s) 123, 650
BmgBI CACGTC 2 cut(s) 60, 161
BmgT120I GGNCC 1 cut(s) 719
BmiI GGNNCC 2 cut(s) 397, 702
BmrFI CCNGG 1 cut(s) 629
BmsI GCATC 2 cut(s) 219, 720
BoxI GACNNNNGTC 1 cut(s) 421
BpmI CTGGAG 2 cut(s) 278, 346
BpuMI CCSGG 1 cut(s) 629
Bsa29I ATCGAT 2 cut(s) 319, 455
BsaBI GATNNNNATC 2 cut(s) 209, 469
BsaHI GRCGYC 1 cut(s) 200
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 2 cut(s) 153, 191
BsaWI WCCGGW 6 cut(s) 446, 475, 482, 503, 510, 517
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bse118I RCCGGY 5 cut(s) 392, 475, 482, 503, 544
Bse1I ACTGG 2 cut(s) 232, 421
Bse8I GATNNNNATC 2 cut(s) 209, 469
BseAI TCCGGA 1 cut(s) 510
BseCI ATCGAT 2 cut(s) 319, 455
BseDI CCNNGG 2 cut(s) 153, 191
BseGI GGATG 1 cut(s) 201
BseJI GATNNNNATC 2 cut(s) 209, 469
BseMII CTCAG 1 cut(s) 136
BseNI ACTGG 2 cut(s) 232, 421
BsePI GCGCGC 1 cut(s) 145
BseRI GAGGAG 4 cut(s) 142, 172, 175, 294
BseX3I CGGCCG 1 cut(s) 371
BseXI GCAGC 3 cut(s) 528, 646, 649
Bsh1236I CGCG 2 cut(s) 145, 147
Bsh1285I CGRYCG 2 cut(s) 374, 487
BshFI GGCC 6 cut(s) 56, 245, 343, 373, 548, 744
BshNI GGYRCC 2 cut(s) 395, 700
BshTI ACCGGT 3 cut(s) 475, 482, 503
BshVI ATCGAT 2 cut(s) 319, 455
BsiEI CGRYCG 2 cut(s) 374, 487
BsiHKAI GWGCWC 1 cut(s) 291
BslFI GGGAC 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 512
BsmFI GGGAC 1 cut(s) 111
BsnI GGCC 6 cut(s) 56, 245, 343, 373, 548, 744
Bso31I GGTCTC 1 cut(s) 512
Bsp1286I GDGCHC 1 cut(s) 291
Bsp13I TCCGGA 1 cut(s) 510
Bsp143I GATC 3 cut(s) 204, 375, 558
BspACI CCGC 5 cut(s) 133, 208, 279, 539, 717
BspANI GGCC 6 cut(s) 56, 245, 343, 373, 548, 744
BspCNI CTCAG 1 cut(s) 135
BspDI ATCGAT 2 cut(s) 319, 455
BspEI TCCGGA 1 cut(s) 510
BspFNI CGCG 2 cut(s) 145, 147
BspLI GGNNCC 2 cut(s) 397, 702
BspMI ACCTGC 1 cut(s) 361
BspPI GGATC 1 cut(s) 199
BspT107I GGYRCC 2 cut(s) 395, 700
BspTNI GGTCTC 1 cut(s) 512
BsrFI RCCGGY 5 cut(s) 392, 475, 482, 503, 544
BsrI ACTGG 2 cut(s) 232, 421
BssAI RCCGGY 5 cut(s) 392, 475, 482, 503, 544
BssECI CCNNGG 2 cut(s) 153, 191
BssHII GCGCGC 1 cut(s) 145
BssMI GATC 3 cut(s) 204, 375, 558
BssNI GRCGYC 1 cut(s) 200
Bst4CI ACNGT 3 cut(s) 366, 695, 751
BstACI GRCGYC 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 147, 546
BstDEI CTNAG 2 cut(s) 122, 647
BstDSI CCRYGG 1 cut(s) 191
BstEII GGTNACC 2 cut(s) 478, 499
BstF5I GGATG 1 cut(s) 201
BstFNI CGCG 2 cut(s) 145, 147
BstHHI GCGC 2 cut(s) 147, 149
BstKTI GATC 3 cut(s) 207, 378, 561
BstMAI GTCTC 1 cut(s) 512
BstMBI GATC 3 cut(s) 204, 375, 558
BstMCI CGRYCG 2 cut(s) 374, 487
BstMWI GCNNNNNNNGC 2 cut(s) 155, 684
BstNSI RCATGY 1 cut(s) 276
BstPAI GACNNNNGTC 1 cut(s) 421
BstPI GGTNACC 2 cut(s) 478, 499
BstSCI CCNGG 1 cut(s) 627
BstUI CGCG 2 cut(s) 145, 147
BstV1I GCAGC 3 cut(s) 528, 646, 649
BstZI CGGCCG 1 cut(s) 371
Bsu15I ATCGAT 2 cut(s) 319, 455
BsuRI GGCC 6 cut(s) 56, 245, 343, 373, 548, 744
BsuTUI ATCGAT 2 cut(s) 319, 455
BtgI CCRYGG 1 cut(s) 191
BtrI CACGTC 2 cut(s) 60, 161
BtsCI GGATG 1 cut(s) 201
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 2 cut(s) 147, 546
CfoI GCGC 2 cut(s) 147, 149
Cfr10I RCCGGY 5 cut(s) 392, 475, 482, 503, 544
Cfr13I GGNCC 1 cut(s) 719
ClaI ATCGAT 2 cut(s) 319, 455
Csp6I GTAC 1 cut(s) 701
CspAI ACCGGT 3 cut(s) 475, 482, 503
CviAII CATG 1 cut(s) 273
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 2 cut(s) 122, 647
DpnI GATC 3 cut(s) 206, 377, 560
DpnII GATC 3 cut(s) 204, 375, 558
DrdI GACNNNNNNGTC 1 cut(s) 79
DriI GACNNNNNGTC 2 cut(s) 123, 650
DseDI GACNNNNNNGTC 1 cut(s) 79
EaeI YGGCCR 4 cut(s) 54, 341, 371, 546
EagI CGGCCG 1 cut(s) 371
Eam1105I GACNNNNNGTC 2 cut(s) 123, 650
EciI GGCGGA 1 cut(s) 732
Ecl136II GAGCTC 1 cut(s) 289
EclXI CGGCCG 1 cut(s) 371
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 512
Eco47I GGWCC 1 cut(s) 719
Eco52I CGGCCG 1 cut(s) 371
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 1 cut(s) 633
Eco91I GGTNACC 2 cut(s) 478, 499
EcoICRI GAGCTC 1 cut(s) 289
EcoO65I GGTNACC 2 cut(s) 478, 499
EcoT38I GRGCYC 1 cut(s) 291
FaeI CATG 1 cut(s) 276
FaiI YATR 2 cut(s) 137, 274
FaqI GGGAC 1 cut(s) 111
FatI CATG 1 cut(s) 272
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 3 cut(s) 542, 635, 638
FokI GGATG 1 cut(s) 208
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 3 cut(s) 542, 635, 638
FspBI CTAG 1 cut(s) 525
GlaI GCGC 2 cut(s) 146, 148
GluI GCNGC 3 cut(s) 542, 635, 638
GsuI CTGGAG 2 cut(s) 278, 346
HaeIII GGCC 6 cut(s) 56, 245, 343, 373, 548, 744
HhaI GCGC 2 cut(s) 147, 149
Hin1I GRCGYC 1 cut(s) 200
Hin1II CATG 1 cut(s) 276
Hin6I GCGC 2 cut(s) 145, 147
HinP1I GCGC 2 cut(s) 145, 147
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 9 cut(s) 118, 321, 410, 436, 443, 465, 514, 535, 604
HphI GGTGA 3 cut(s) 465, 472, 493
Hpy166II GTNNAC 2 cut(s) 108, 270
Hpy188I TCNGA 6 cut(s) 215, 427, 464, 609, 731, 755
Hpy188III TCNNGA 5 cut(s) 257, 325, 414, 440, 511
Hpy8I GTNNAC 2 cut(s) 108, 270
Hpy99I CGWCG 5 cut(s) 103, 106, 165, 205, 659
HpyAV CCTTC 2 cut(s) 50, 668
HpyCH4III ACNGT 3 cut(s) 366, 695, 751
HpyCH4IV ACGT 3 cut(s) 59, 160, 200
HpyCH4V TGCA 3 cut(s) 276, 350, 356
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 684
HpyF3I CTNAG 2 cut(s) 122, 647
HpySE526I ACGT 3 cut(s) 59, 160, 200
Hsp92I GRCGYC 1 cut(s) 200
Hsp92II CATG 1 cut(s) 276
HspAI GCGC 2 cut(s) 145, 147
Kpn2I TCCGGA 1 cut(s) 510
KpnI GGTACC 1 cut(s) 704
KroI GCCGGC 1 cut(s) 544
KroNI GCCGGC 1 cut(s) 546
Kzo9I GATC 3 cut(s) 204, 375, 558
LmnI GCTCC 3 cut(s) 294, 327, 631
Lsp1109I GCAGC 3 cut(s) 528, 646, 649
LweI GCATC 2 cut(s) 219, 720
MaeI CTAG 1 cut(s) 525
MaeII ACGT 3 cut(s) 59, 160, 200
MaeIII GTNAC 5 cut(s) 478, 499, 520, 616, 689
MalI GATC 3 cut(s) 206, 377, 560
MboI GATC 3 cut(s) 204, 375, 558
MboII GAAGA 5 cut(s) 3, 35, 38, 57, 78
MhlI GDGCHC 1 cut(s) 291
MlsI TGGCCA 2 cut(s) 56, 343
MluCI AATT 2 cut(s) 357, 725
MluNI TGGCCA 2 cut(s) 56, 343
MlyI GAGTC 3 cut(s) 112, 544, 613
MmeI TCCRAC 2 cut(s) 442, 587
Mox20I TGGCCA 2 cut(s) 56, 343
MroI TCCGGA 1 cut(s) 510
MroNI GCCGGC 1 cut(s) 544
MscI TGGCCA 2 cut(s) 56, 343
Msp20I TGGCCA 2 cut(s) 56, 343
MspA1I CMGCKG 1 cut(s) 541
MspR9I CCNGG 1 cut(s) 629
MvnI CGCG 2 cut(s) 145, 147
MwoI GCNNNNNNNGC 2 cut(s) 155, 684
NaeI GCCGGC 1 cut(s) 546
NciI CCSGG 1 cut(s) 629
NdeII GATC 3 cut(s) 204, 375, 558
NgoMIV GCCGGC 1 cut(s) 544
NlaIII CATG 1 cut(s) 276
NlaIV GGNNCC 2 cut(s) 397, 702
NmeAIII GCCGAG 2 cut(s) 134, 691
NmuCI GTSAC 4 cut(s) 478, 499, 520, 616
NspI RCATGY 1 cut(s) 276
PaqCI CACCTGC 1 cut(s) 361
PauI GCGCGC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 5 cut(s) 77, 95, 107, 567, 663
PdiI GCCGGC 1 cut(s) 546
PfeI GAWTC 6 cut(s) 321, 410, 436, 443, 465, 514
PflMI CCANNNNNTGG 1 cut(s) 740
PfoI TCCNGGA 1 cut(s) 627
PinAI ACCGGT 3 cut(s) 475, 482, 503
PkrI GCNGC 3 cut(s) 543, 636, 639
PleI GAGTC 3 cut(s) 112, 543, 612
PpsI GAGTC 3 cut(s) 112, 543, 612
PshAI GACNNNNGTC 1 cut(s) 421
Psp124BI GAGCTC 1 cut(s) 291
PspEI GGTNACC 2 cut(s) 478, 499
PspN4I GGNNCC 2 cut(s) 397, 702
PspPI GGNCC 1 cut(s) 719
PteI GCGCGC 1 cut(s) 145
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
SacI GAGCTC 1 cut(s) 291
SalI GTCGAC 1 cut(s) 268
SatI GCNGC 3 cut(s) 542, 635, 638
Sau3AI GATC 3 cut(s) 204, 375, 558
Sau96I GGNCC 1 cut(s) 719
SchI GAGTC 3 cut(s) 112, 544, 613
ScrFI CCNGG 1 cut(s) 629
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 2 cut(s) 219, 720
SgrAI CRCCGGYG 1 cut(s) 392
SinI GGWCC 1 cut(s) 719
SpeI ACTAGT 1 cut(s) 524
Sse9I AATT 2 cut(s) 357, 725
SsiI CCGC 5 cut(s) 133, 208, 279, 539, 717
SspI AATATT 1 cut(s) 739
SspMI CTAG 1 cut(s) 525
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 627
TaaI ACNGT 3 cut(s) 366, 695, 751
TaiI ACGT 3 cut(s) 62, 163, 203
TaqI TCGA 9 cut(s) 71, 203, 236, 269, 319, 455, 570, 591, 657
TaqII GACCGA 2 cut(s) 487, 551
TasI AATT 2 cut(s) 357, 725
TfiI GAWTC 6 cut(s) 321, 410, 436, 443, 465, 514
TseFI GTSAC 4 cut(s) 478, 499, 520, 616
TseI GCWGC 3 cut(s) 541, 634, 637
Tsp45I GTSAC 4 cut(s) 478, 499, 520, 616
TspDTI ATGAA 3 cut(s) 57, 609, 675
TspGWI ACGGA 1 cut(s) 569
Van91I CCANNNNNTGG 1 cut(s) 740
VpaK11BI GGWCC 1 cut(s) 719
XceI RCATGY 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 269
XspI CTAG 1 cut(s) 525
ZraI GACGTC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.