Rroxscaffold_4G00307130

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002289
Physical Location & Seq
Forward (+)
28219337 .. 28222660
3324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00307130.1

Sequence Viewer

Length: 594 bp
ATGGCTTCTTCAATTCACTACGATATTCGCTGCGTGCTTCTTCTTCACTTGGCCACGTCATCGTTATCGTCATCATCGTCACCTTCGACGTCGTTCCGCTTCAGAGTTTTCTGCAATGACGAAGCGAAAGGGGCCGTGAAGCTTCCGGAGAAGCCGTTGATATGCACCGCCGACGAGCTCCACTACGTCTCCGTGCCCAACTCCGATTGGAGGCTCGCCCTCTTATTGTCTTTAGCTTCACCTATCATCTATTTGCTTTACGGTTATCTAGATGTTAGATTATTTGGCTTCTATGATCTACTGTTGTCTTGTACCTTGGAAAATGTTACTTATGCTTCTGGTGCCTTTTCTGATTTGGATACCCAGCTTCATTTTTCTTTCAGTCACTCAACTATTGAAGATGATGAGCTCCCAGAATACCTTGGTGATGGAGTTACTACGCCATTCCAATCTGCATCTTCGGCATTGCATATGGAAGCCGAGGCATTAAGGGCAGGTCTATTGCTGGCTATTCACCAAGGCTGGATGGATATTGAAATGGACACCGATTGTGCTATTCTAGTTTATGCCCTCAATAGGGAAGATGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

197

Amino Acids

21.86

Weight (kDa)

4.43

Isoelectric Point (pI)

50.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 145 - 195 3.3e-06 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 92
Acc36I ACCTGC 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 341
AccIII TCCGGA 1 cut(s) 145
AciI CCGC 2 cut(s) 97, 168
AcoI YGGCCR 1 cut(s) 51
AcuI CTGAAG 1 cut(s) 85
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 1 cut(s) 313
AfiI CCNNNNNNNGG 3 cut(s) 210, 576, 577
AgsI TTSAA 3 cut(s) 12, 398, 536
AjiI CACGTC 1 cut(s) 57
AluBI AGCT 5 cut(s) 142, 178, 236, 367, 409
AluI AGCT 5 cut(s) 142, 178, 236, 367, 409
Alw21I GWGCWC 2 cut(s) 180, 411
Alw26I GTCTC 1 cut(s) 193
Aor13HI TCCGGA 1 cut(s) 145
AoxI GGCC 2 cut(s) 51, 132
ApeKI GCWGC 1 cut(s) 30
AspS9I GGNCC 1 cut(s) 132
AsuHPI GGTGA 4 cut(s) 72, 231, 437, 506
BaeGI GKGCMC 1 cut(s) 198
BalI TGGCCA 1 cut(s) 53
BanI GGYRCC 1 cut(s) 341
BanII GRGCYC 2 cut(s) 180, 411
Bbv12I GWGCWC 2 cut(s) 180, 411
BbvI GCAGC 1 cut(s) 17
BccI CCATC 2 cut(s) 422, 520
BceAI ACGGC 2 cut(s) 119, 139
BciVI GTATCC 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 2 cut(s) 269, 560
BfuAI ACCTGC 1 cut(s) 485
BfuI GTATCC 1 cut(s) 352
BisI GCNGC 1 cut(s) 31
BlsI GCNGC 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 132
BmiI GGNNCC 2 cut(s) 133, 343
BmsI GCATC 1 cut(s) 464
BsaHI GRCGYC 1 cut(s) 89
BsaJI CCNNGG 4 cut(s) 315, 421, 480, 517
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 173, 203
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 576, 577
Bse3DI GCAATG 2 cut(s) 121, 464
BseAI TCCGGA 1 cut(s) 145
BseDI CCNNGG 4 cut(s) 315, 421, 480, 517
BseGI GGATG 1 cut(s) 531
BseLI CCNNNNNNNGG 3 cut(s) 210, 576, 577
BseMI GCAATG 2 cut(s) 121, 464
BseSI GKGCMC 1 cut(s) 198
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 363
BshFI GGCC 2 cut(s) 53, 134
BshNI GGYRCC 1 cut(s) 341
BsiHKAI GWGCWC 2 cut(s) 180, 411
BsiSI CCGG 1 cut(s) 146
BslI CCNNNNNNNGG 3 cut(s) 210, 576, 577
BsmAI GTCTC 1 cut(s) 193
BsmBI CGTCTC 1 cut(s) 193
BsnI GGCC 2 cut(s) 53, 134
Bsp1286I GDGCHC 3 cut(s) 180, 198, 411
Bsp13I TCCGGA 1 cut(s) 145
Bsp143I GATC 1 cut(s) 295
BspACI CCGC 2 cut(s) 97, 168
BspANI GGCC 2 cut(s) 53, 134
BspEI TCCGGA 1 cut(s) 145
BspLI GGNNCC 2 cut(s) 133, 343
BspMI ACCTGC 1 cut(s) 485
BspT107I GGYRCC 1 cut(s) 341
BsrDI GCAATG 2 cut(s) 121, 464
BssECI CCNNGG 4 cut(s) 315, 421, 480, 517
BssMI GATC 1 cut(s) 295
BssNI GRCGYC 1 cut(s) 89
BssT1I CCWWGG 3 cut(s) 315, 421, 517
Bst4CI ACNGT 2 cut(s) 263, 303
BstACI GRCGYC 1 cut(s) 89
BstC8I GCNNGC 3 cut(s) 35, 216, 507
BstF5I GGATG 1 cut(s) 531
BstKTI GATC 1 cut(s) 298
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 1 cut(s) 295
BstMWI GCNNNNNNNGC 4 cut(s) 131, 341, 461, 491
BstSLI GKGCMC 1 cut(s) 198
BstV1I GCAGC 1 cut(s) 17
BsuI GTATCC 1 cut(s) 352
BsuRI GGCC 2 cut(s) 53, 134
BtrI CACGTC 1 cut(s) 57
BtsCI GGATG 1 cut(s) 531
BveI ACCTGC 1 cut(s) 485
Cac8I GCNNGC 3 cut(s) 35, 216, 507
Cfr13I GGNCC 1 cut(s) 132
Csp6I GTAC 1 cut(s) 312
CviQI GTAC 1 cut(s) 312
DpnI GATC 1 cut(s) 297
DpnII GATC 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 51
Ecl136II GAGCTC 2 cut(s) 178, 409
Eco130I CCWWGG 3 cut(s) 315, 421, 517
Eco24I GRGCYC 2 cut(s) 180, 411
Eco53kI GAGCTC 2 cut(s) 178, 409
Eco57I CTGAAG 1 cut(s) 85
EcoICRI GAGCTC 2 cut(s) 178, 409
EcoT14I CCWWGG 3 cut(s) 315, 421, 517
EcoT38I GRGCYC 2 cut(s) 180, 411
ErhI CCWWGG 3 cut(s) 315, 421, 517
Esp3I CGTCTC 1 cut(s) 193
FaiI YATR 6 cut(s) 163, 294, 333, 471, 473, 567
FauNDI CATATG 1 cut(s) 471
Fnu4HI GCNGC 1 cut(s) 31
FokI GGATG 1 cut(s) 538
FriOI GRGCYC 2 cut(s) 180, 411
Fsp4HI GCNGC 1 cut(s) 31
FspBI CTAG 2 cut(s) 269, 560
GluI GCNGC 1 cut(s) 31
GsaI CCCAGC 1 cut(s) 367
HaeIII GGCC 2 cut(s) 53, 134
HapII CCGG 1 cut(s) 146
Hin1I GRCGYC 1 cut(s) 89
HindIII AAGCTT 1 cut(s) 140
HpaII CCGG 1 cut(s) 146
HphI GGTGA 4 cut(s) 72, 231, 437, 506
Hpy188I TCNGA 3 cut(s) 104, 205, 352
Hpy188III TCNNGA 2 cut(s) 146, 269
Hpy99I CGWCG 3 cut(s) 91, 94, 176
HpyAV CCTTC 1 cut(s) 93
HpyCH4III ACNGT 2 cut(s) 263, 303
HpyCH4IV ACGT 3 cut(s) 56, 89, 186
HpyCH4V TGCA 4 cut(s) 114, 165, 455, 469
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 341, 461, 491
HpySE526I ACGT 3 cut(s) 56, 89, 186
Hsp92I GRCGYC 1 cut(s) 89
Kpn2I TCCGGA 1 cut(s) 145
Kzo9I GATC 1 cut(s) 295
LmnI GCTCC 2 cut(s) 183, 414
LpnPI CCDG 7 cut(s) 159, 324, 377, 426, 480, 491, 508
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 464
MaeI CTAG 2 cut(s) 269, 560
MaeII ACGT 3 cut(s) 56, 89, 186
MaeIII GTNAC 4 cut(s) 78, 325, 383, 433
MalI GATC 1 cut(s) 297
MboI GATC 1 cut(s) 295
MboII GAAGA 5 cut(s) 32, 35, 410, 450, 593
MhlI GDGCHC 3 cut(s) 180, 198, 411
MlsI TGGCCA 1 cut(s) 53
MluCI AATT 1 cut(s) 12
MluNI TGGCCA 1 cut(s) 53
MnlI CCTC 4 cut(s) 204, 230, 475, 581
Mox20I TGGCCA 1 cut(s) 53
MroI TCCGGA 1 cut(s) 145
MscI TGGCCA 1 cut(s) 53
MseI TTAA 1 cut(s) 488
Msp20I TGGCCA 1 cut(s) 53
MspI CCGG 1 cut(s) 146
MwoI GCNNNNNNNGC 4 cut(s) 131, 341, 461, 491
NdeI CATATG 1 cut(s) 471
NdeII GATC 1 cut(s) 295
NlaIV GGNNCC 2 cut(s) 133, 343
NmeAIII GCCGAG 1 cut(s) 505
NmuCI GTSAC 2 cut(s) 78, 383
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 83
PkrI GCNGC 1 cut(s) 32
Psp124BI GAGCTC 2 cut(s) 180, 411
PspFI CCCAGC 1 cut(s) 363
PspN4I GGNNCC 2 cut(s) 133, 343
PspPI GGNCC 1 cut(s) 132
RsaI GTAC 1 cut(s) 313
RsaNI GTAC 1 cut(s) 312
SacI GAGCTC 2 cut(s) 180, 411
SaqAI TTAA 1 cut(s) 488
SatI GCNGC 1 cut(s) 31
Sau3AI GATC 1 cut(s) 295
Sau96I GGNCC 1 cut(s) 132
SduI GDGCHC 3 cut(s) 180, 198, 411
SfaNI GCATC 1 cut(s) 464
Sse9I AATT 1 cut(s) 12
SsiI CCGC 2 cut(s) 97, 168
SspMI CTAG 2 cut(s) 269, 560
SstI GAGCTC 2 cut(s) 180, 411
StyI CCWWGG 3 cut(s) 315, 421, 517
TaaI ACNGT 2 cut(s) 263, 303
TaiI ACGT 3 cut(s) 59, 92, 189
TaqI TCGA 1 cut(s) 86
TasI AATT 1 cut(s) 12
Tru1I TTAA 1 cut(s) 488
Tru9I TTAA 1 cut(s) 488
TseFI GTSAC 2 cut(s) 78, 383
TseI GCWGC 1 cut(s) 30
Tsp45I GTSAC 2 cut(s) 78, 383
TspDTI ATGAA 1 cut(s) 359
TspGWI ACGGA 1 cut(s) 181
XbaI TCTAGA 1 cut(s) 268
XspI CTAG 2 cut(s) 269, 560
ZraI GACGTC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.