FvH4_5g39020

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003291
Physical Location & Seq
Forward (+)
28820265 .. 28820738
474 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g39020.t1

Sequence Viewer

Length: 474 bp
ATGGCTCAGTCGAGTTCTCTATCTGCAGAAACCGAGTCACTGAGTCAGGTCCTGGGCCTTATCGAAACATTCAAAGCTTTCGATTCAGACAACGATGGGAAAATCACAGTAGCAGAGCTCGGTGGGATCCTAGGATCGCTTGGTTACAGTTCGAATGAGCAAGATGTGAGGGCAATGATGGAGCAAGGGGACACAAACAAAGATGGGTTGCTGAGCATAGAGGAGTTTCTGGAGATGAACACAAGGAACATGGAATTCGGGGGGCTTGAAAATGTGCTAAAGAATGCTTTTGAGGCCTTGGATGTTGATGGGGATGAGGTTGTGACTGCAGAGGAGTTGTATGAGGTTATGGGAGATGAGATGGGGTTTGAGTTAAGTCTGGAGGATTGCCAGGGTATTATTGCTTCTATAGATTCAGATGGAGATGGGGCTGTTAGTTTTGACGATTTCAAACTTATAGTTAATTCCCTCTAA

Protein Analysis

158

Amino Acids

16.9

Weight (kDa)

4.05

Isoelectric Point (pI)

32.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 20 - 79 3.5e-13 EF-hand domain pair
EF-hand_6 PF13405 20 - 48 1.3e-06 EF-hand domain
EF-hand_1 PF00036 21 - 45 8.7e-06 EF hand domain
EF-hand_5 PF13202 21 - 41 8e-06 EF hand
EF-hand_8 PF13833 32 - 82 3e-07 EF-hand domain pair
EF-hand_7 PF13499 90 - 151 4.3e-12 EF-hand domain pair
EF-hand_8 PF13833 105 - 151 1.5e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 121, 134, 142
AcsI RAATTY 1 cut(s) 254
AgsI TTSAA 3 cut(s) 73, 269, 451
AjnI CCWGG 2 cut(s) 51, 390
AluBI AGCT 2 cut(s) 77, 118
AluI AGCT 2 cut(s) 77, 118
Alw21I GWGCWC 1 cut(s) 120
AlwI GGATC 3 cut(s) 121, 134, 142
AlwNI CAGNNNCTG 1 cut(s) 52
AoxI GGCC 2 cut(s) 55, 294
ApoI RAATTY 1 cut(s) 254
AspA2I CCTAGG 1 cut(s) 130
AspS9I GGNCC 2 cut(s) 49, 55
AsuII TTCGAA 1 cut(s) 152
AvaII GGWCC 1 cut(s) 49
AvrII CCTAGG 1 cut(s) 130
BamHI GGATCC 1 cut(s) 126
BanII GRGCYC 1 cut(s) 120
BarI GAAGNNNNNNTAC 2 cut(s) 388, 420
Bbv12I GWGCWC 1 cut(s) 120
BccI CCATC 7 cut(s) 89, 172, 197, 302, 355, 413, 419
BciT130I CCWGG 2 cut(s) 53, 392
BfaI CTAG 1 cut(s) 131
BfmI CTRYAG 3 cut(s) 24, 327, 408
BlnI CCTAGG 1 cut(s) 130
BlpI GCTNAGC 1 cut(s) 212
Bme1390I CCNGG 2 cut(s) 53, 392
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 2 cut(s) 49, 55
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 2 cut(s) 53, 392
BpmI CTGGAG 2 cut(s) 251, 401
Bpu1102I GCTNAGC 1 cut(s) 212
Bpu14I TTCGAA 1 cut(s) 152
BsaJI CCNNGG 4 cut(s) 52, 130, 297, 391
Bse3DI GCAATG 1 cut(s) 180
BseBI CCWGG 2 cut(s) 53, 392
BseDI CCNNGG 4 cut(s) 52, 130, 297, 391
BseGI GGATG 2 cut(s) 307, 319
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 3 cut(s) 20, 32, 203
BseRI GAGGAG 2 cut(s) 236, 347
BshFI GGCC 2 cut(s) 57, 296
BsiHKAI GWGCWC 1 cut(s) 120
BslFI GGGAC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 203
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 2 cut(s) 57, 296
Bsp119I TTCGAA 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 2 cut(s) 126, 134
Bsp1720I GCTNAGC 1 cut(s) 212
BspANI GGCC 2 cut(s) 57, 296
BspCNI CTCAG 3 cut(s) 19, 33, 204
BspLI GGNNCC 1 cut(s) 128
BspMAI CTGCAG 2 cut(s) 28, 331
BspPI GGATC 3 cut(s) 121, 134, 142
BspT104I TTCGAA 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 180
BssECI CCNNGG 4 cut(s) 52, 130, 297, 391
BssMI GATC 2 cut(s) 126, 134
BssT1I CCWWGG 2 cut(s) 130, 297
Bst2UI CCWGG 2 cut(s) 53, 392
Bst4CI ACNGT 2 cut(s) 109, 149
BstBI TTCGAA 1 cut(s) 152
BstDEI CTNAG 3 cut(s) 6, 41, 212
BstF5I GGATG 2 cut(s) 307, 319
BstKTI GATC 2 cut(s) 129, 137
BstMBI GATC 2 cut(s) 126, 134
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 2 cut(s) 53, 392
BstSCI CCNGG 2 cut(s) 51, 390
BstSFI CTRYAG 3 cut(s) 24, 327, 408
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 2 cut(s) 57, 296
BtsCI GGATG 2 cut(s) 307, 319
BtsIMutI CAGTG 1 cut(s) 38
CaiI CAGNNNCTG 1 cut(s) 52
Cfr13I GGNCC 2 cut(s) 49, 55
CviAII CATG 1 cut(s) 250
CviJI RGCY 7 cut(s) 5, 57, 77, 118, 265, 296, 431
CviKI_1 RGCY 7 cut(s) 5, 57, 77, 118, 265, 296, 431
DdeI CTNAG 3 cut(s) 6, 41, 212
DpnI GATC 2 cut(s) 128, 136
DpnII GATC 2 cut(s) 126, 134
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 130, 297
Eco147I AGGCCT 1 cut(s) 296
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 118
EcoO109I RGGNCCY 1 cut(s) 49
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 51, 390
EcoT14I CCWWGG 2 cut(s) 130, 297
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 2 cut(s) 130, 297
FaeI CATG 1 cut(s) 253
FaiI YATR 6 cut(s) 218, 251, 342, 350, 410, 458
FaqI GGGAC 1 cut(s) 203
FatI CATG 1 cut(s) 249
FokI GGATG 2 cut(s) 314, 326
FriOI GRGCYC 1 cut(s) 120
FspBI CTAG 1 cut(s) 131
GsuI CTGGAG 2 cut(s) 251, 401
HaeIII GGCC 2 cut(s) 57, 296
Hin1II CATG 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 4 cut(s) 35, 43, 83, 413
Hpy188I TCNGA 2 cut(s) 88, 418
Hpy188III TCNNGA 2 cut(s) 230, 380
HpyCH4III ACNGT 2 cut(s) 109, 149
HpyCH4V TGCA 2 cut(s) 26, 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 3 cut(s) 6, 41, 212
Hsp92II CATG 1 cut(s) 253
Kzo9I GATC 2 cut(s) 126, 134
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 7 cut(s) 32, 38, 65, 215, 365, 377, 404
MaeI CTAG 1 cut(s) 131
MaeIII GTNAC 3 cut(s) 36, 143, 322
MalI GATC 2 cut(s) 128, 136
MboI GATC 2 cut(s) 126, 134
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 2 cut(s) 254, 463
MlyI GAGTC 2 cut(s) 44, 52
MnlI CCTC 7 cut(s) 162, 214, 286, 310, 325, 337, 376
MseI TTAA 2 cut(s) 374, 462
MspR9I CCNGG 2 cut(s) 53, 392
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 2 cut(s) 53, 392
MwoI GCNNNNNNNGC 1 cut(s) 293
NdeII GATC 2 cut(s) 126, 134
NlaIII CATG 1 cut(s) 253
NlaIV GGNNCC 1 cut(s) 128
NmuCI GTSAC 2 cut(s) 36, 322
NspV TTCGAA 1 cut(s) 152
PceI AGGCCT 1 cut(s) 296
PctI GAATGC 1 cut(s) 289
PfeI GAWTC 2 cut(s) 83, 413
PleI GAGTC 2 cut(s) 43, 51
PpsI GAGTC 2 cut(s) 43, 51
PpuMI RGGWCCY 1 cut(s) 49
Psp124BI GAGCTC 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 49
Psp6I CCWGG 2 cut(s) 51, 390
PspGI CCWGG 2 cut(s) 51, 390
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 2 cut(s) 49, 55
PspPPI RGGWCCY 1 cut(s) 49
PstI CTGCAG 2 cut(s) 28, 331
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 1 cut(s) 126
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 2 cut(s) 374, 462
Sau3AI GATC 2 cut(s) 126, 134
Sau96I GGNCC 2 cut(s) 49, 55
SchI GAGTC 2 cut(s) 44, 52
ScrFI CCNGG 2 cut(s) 53, 392
SduI GDGCHC 1 cut(s) 120
SetI ASST 5 cut(s) 51, 79, 120, 321, 348
SfcI CTRYAG 3 cut(s) 24, 327, 408
SfuI TTCGAA 1 cut(s) 152
SinI GGWCC 1 cut(s) 49
Sse9I AATT 2 cut(s) 254, 463
SseBI AGGCCT 1 cut(s) 296
SspMI CTAG 1 cut(s) 131
SstI GAGCTC 1 cut(s) 120
StuI AGGCCT 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 51, 390
StyI CCWWGG 2 cut(s) 130, 297
TaaI ACNGT 2 cut(s) 109, 149
TaqI TCGA 4 cut(s) 11, 63, 81, 152
TasI AATT 2 cut(s) 254, 463
TfiI GAWTC 2 cut(s) 83, 413
Tru1I TTAA 2 cut(s) 374, 462
Tru9I TTAA 2 cut(s) 374, 462
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 2 cut(s) 36, 322
Tsp45I GTSAC 2 cut(s) 36, 322
TspDTI ATGAA 1 cut(s) 251
TspRI CASTG 1 cut(s) 45
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 1 cut(s) 254
XmaJI CCTAGG 1 cut(s) 130
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.