RLG00000022992

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003291
Physical Location & Seq
Forward (+)
16360128 .. 16360608
481 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022992

Sequence Viewer

Length: 333 bp
ATGGGTTTCAAATTCCTTTTCTCCCCGAAGAAGAAATCCACCCCCTCCAGCGCCGGCGCCGCTCAGCCGACGGTGATGCTGTCACCGTCGTTCAACACCCAGGCGCAGATCGTCAAGCTGGAGCTGGTCTTCAAGAAATTCGACGTTAACGGCAACGGCAAGATCTCATCGTCGGAGCTCGGAGAGATCATGGACAGCCTGGGCCACCCGGCGACAGAGGACGAGCTCCGGTCCATGATCCTCGAGGTCGACGCTGACGGCAACGGCTACATCGACTTCAAGGAGTTCGTGGAGCTCAACACCAACGGGATCGACTCCGTCGAGGCGTTGTAG

Protein Analysis

111

Amino Acids

11.92

Weight (kDa)

4.64

Isoelectric Point (pI)

25.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 39 - 99 1.4e-14 EF-hand domain pair
EF-hand_1 PF00036 40 - 66 4.8e-07 EF hand domain
EF-hand_6 PF13405 40 - 68 4.2e-07 EF-hand domain
EF-hand_5 PF13202 41 - 64 6.9e-07 EF hand
AIF-1 PF21008 43 - 99 1.2e-06 Allograft inflammatory factor 1
EF-hand_8 PF13833 52 - 99 1.5e-08 EF-hand domain pair
EF-hand_1 PF00036 75 - 99 2.6e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 56
AccBSI CCGCTC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 249
AciI CCGC 1 cut(s) 60
AclWI GGATC 2 cut(s) 232, 317
AcsI RAATTY 2 cut(s) 11, 137
AcyI GRCGYC 1 cut(s) 57
AgsI TTSAA 4 cut(s) 10, 94, 133, 280
AjnI CCWGG 2 cut(s) 99, 198
AluBI AGCT 5 cut(s) 118, 124, 178, 226, 295
AluI AGCT 5 cut(s) 118, 124, 178, 226, 295
Alw21I GWGCWC 3 cut(s) 180, 228, 297
AlwI GGATC 2 cut(s) 232, 317
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 1 cut(s) 202
ApoI RAATTY 2 cut(s) 11, 137
AspLEI GCGC 3 cut(s) 53, 59, 106
AspS9I GGNCC 2 cut(s) 202, 231
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 2 cut(s) 75, 85
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 1 cut(s) 231
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 3 cut(s) 180, 228, 297
BbsI GAAGAC 1 cut(s) 121
Bbv12I GWGCWC 3 cut(s) 180, 228, 297
BceAI ACGGC 4 cut(s) 166, 172, 274, 280
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 2 cut(s) 101, 200
BcnI CCSGG 1 cut(s) 209
BfoI RGCGCY 2 cut(s) 54, 60
BglII AGATCT 1 cut(s) 162
BisI GCNGC 1 cut(s) 60
BlpI GCTNAGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 61
Bme1390I CCNGG 3 cut(s) 101, 200, 209
Bme18I GGWCC 1 cut(s) 231
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 2 cut(s) 202, 231
BmiI GGNNCC 1 cut(s) 58
BmrFI CCNGG 3 cut(s) 101, 200, 209
BmsI GCATC 1 cut(s) 66
BpiI GAAGAC 1 cut(s) 121
BpmI CTGGAG 2 cut(s) 31, 140
Bpu1102I GCTNAGC 1 cut(s) 63
BpuMI CCSGG 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 2 cut(s) 99, 199
BsaWI WCCGGW 1 cut(s) 228
Bse118I RCCGGY 1 cut(s) 53
BseBI CCWGG 2 cut(s) 101, 200
BseDI CCNNGG 2 cut(s) 99, 199
BseMII CTCAG 1 cut(s) 77
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 56
BsiHKAI GWGCWC 3 cut(s) 180, 228, 297
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 3 cut(s) 54, 209, 229
BsnI GGCC 1 cut(s) 204
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 3 cut(s) 180, 228, 297
Bsp143I GATC 5 cut(s) 108, 162, 186, 237, 309
Bsp1720I GCTNAGC 1 cut(s) 63
BspACI CCGC 1 cut(s) 60
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 58
BspPI GGATC 2 cut(s) 232, 317
BspT107I GGYRCC 1 cut(s) 56
BsrBI CCGCTC 1 cut(s) 62
BsrFI RCCGGY 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 2 cut(s) 99, 199
BssMI GATC 5 cut(s) 108, 162, 186, 237, 309
BssNI GRCGYC 1 cut(s) 57
Bst2UI CCWGG 2 cut(s) 101, 200
Bst4CI ACNGT 2 cut(s) 73, 87
BstACI GRCGYC 1 cut(s) 57
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 1 cut(s) 63
BstH2I RGCGCY 2 cut(s) 54, 60
BstHHI GCGC 3 cut(s) 53, 59, 106
BstKTI GATC 5 cut(s) 111, 165, 189, 240, 312
BstMBI GATC 5 cut(s) 108, 162, 186, 237, 309
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 2 cut(s) 101, 200
BstSCI CCNGG 3 cut(s) 99, 198, 207
BstV2I GAAGAC 1 cut(s) 121
BstX2I RGATCY 1 cut(s) 162
BstYI RGATCY 1 cut(s) 162
BsuRI GGCC 1 cut(s) 204
Cac8I GCNNGC 1 cut(s) 55
CfoI GCGC 3 cut(s) 53, 59, 106
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 202, 231
CseI GACGC 1 cut(s) 260
CviAII CATG 2 cut(s) 190, 235
CviJI RGCY 9 cut(s) 67, 118, 124, 178, 198, 204, 226, 267, 295
CviKI_1 RGCY 9 cut(s) 67, 118, 124, 178, 198, 204, 226, 267, 295
DdeI CTNAG 1 cut(s) 63
DinI GGCGCC 1 cut(s) 58
DpnI GATC 5 cut(s) 110, 164, 188, 239, 311
DpnII GATC 5 cut(s) 108, 162, 186, 237, 309
Ecl136II GAGCTC 3 cut(s) 178, 226, 295
Eco24I GRGCYC 3 cut(s) 180, 228, 297
Eco47I GGWCC 1 cut(s) 231
Eco53kI GAGCTC 3 cut(s) 178, 226, 295
Eco88I CYCGRG 1 cut(s) 242
EcoICRI GAGCTC 3 cut(s) 178, 226, 295
EcoRII CCWGG 2 cut(s) 99, 198
EcoT38I GRGCYC 3 cut(s) 180, 228, 297
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
FaeI CATG 2 cut(s) 193, 238
FaiI YATR 2 cut(s) 191, 236
FatI CATG 2 cut(s) 189, 234
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 1 cut(s) 60
FriOI GRGCYC 3 cut(s) 180, 228, 297
Fsp4HI GCNGC 1 cut(s) 60
GlaI GCGC 3 cut(s) 52, 58, 105
GluI GCNGC 1 cut(s) 60
GsuI CTGGAG 2 cut(s) 31, 140
HaeII RGCGCY 2 cut(s) 54, 60
HaeIII GGCC 1 cut(s) 204
HapII CCGG 3 cut(s) 54, 209, 229
HgaI GACGC 1 cut(s) 260
HhaI GCGC 3 cut(s) 53, 59, 106
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 2 cut(s) 193, 238
Hin6I GCGC 3 cut(s) 51, 57, 104
HinP1I GCGC 3 cut(s) 51, 57, 104
HincII GTYRAC 2 cut(s) 148, 250
HindII GTYRAC 2 cut(s) 148, 250
HinfI GANTC 1 cut(s) 314
HpaI GTTAAC 1 cut(s) 148
HpaII CCGG 3 cut(s) 54, 209, 229
HphI GGTGA 2 cut(s) 75, 85
Hpy166II GTNNAC 2 cut(s) 148, 250
Hpy188I TCNGA 2 cut(s) 175, 182
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 2 cut(s) 148, 250
Hpy99I CGWCG 6 cut(s) 73, 91, 146, 175, 254, 323
HpyCH4III ACNGT 2 cut(s) 73, 87
HpyCH4IV ACGT 1 cut(s) 144
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 2 cut(s) 193, 238
HspAI GCGC 3 cut(s) 51, 57, 104
KasI GGCGCC 1 cut(s) 56
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 5 cut(s) 108, 162, 186, 237, 309
LmnI GCTCC 4 cut(s) 121, 175, 231, 292
LweI GCATC 1 cut(s) 66
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 1 cut(s) 81
MalI GATC 5 cut(s) 110, 164, 188, 239, 311
MbiI CCGCTC 1 cut(s) 62
MboI GATC 5 cut(s) 108, 162, 186, 237, 309
MboII GAAGA 3 cut(s) 40, 43, 121
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 3 cut(s) 180, 228, 297
MluCI AATT 2 cut(s) 11, 137
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 5 cut(s) 55, 211, 238, 251, 316
MreI CGCCGGCG 1 cut(s) 53
MroNI GCCGGC 1 cut(s) 53
MseI TTAA 1 cut(s) 147
MspI CCGG 3 cut(s) 54, 209, 229
MspR9I CCNGG 3 cut(s) 101, 200, 209
MvaI CCWGG 2 cut(s) 101, 200
MwoI GCNNNNNNNGC 1 cut(s) 59
NaeI GCCGGC 1 cut(s) 55
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 1 cut(s) 209
NdeII GATC 5 cut(s) 108, 162, 186, 237, 309
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 2 cut(s) 193, 238
NlaIV GGNNCC 1 cut(s) 58
NmuCI GTSAC 1 cut(s) 81
PaeR7I CTCGAG 1 cut(s) 242
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 318
PdiI GCCGGC 1 cut(s) 55
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 1 cut(s) 61
PleI GAGTC 1 cut(s) 308
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 308
Psp124BI GAGCTC 3 cut(s) 180, 228, 297
Psp6I CCWGG 2 cut(s) 99, 198
PspGI CCWGG 2 cut(s) 99, 198
PspN4I GGNNCC 1 cut(s) 58
PspPI GGNCC 2 cut(s) 202, 231
PspXI VCTCGAGB 1 cut(s) 242
PsuI RGATCY 1 cut(s) 162
PsyI GACNNNGTC 1 cut(s) 317
SacI GAGCTC 3 cut(s) 180, 228, 297
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 1 cut(s) 60
Sau3AI GATC 5 cut(s) 108, 162, 186, 237, 309
Sau96I GGNCC 2 cut(s) 202, 231
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 3 cut(s) 101, 200, 209
SduI GDGCHC 3 cut(s) 180, 228, 297
SetI ASST 7 cut(s) 120, 126, 147, 180, 228, 249, 297
SfaNI GCATC 1 cut(s) 66
SfoI GGCGCC 1 cut(s) 58
Sfr274I CTCGAG 1 cut(s) 242
SgrAI CRCCGGYG 1 cut(s) 53
SinI GGWCC 1 cut(s) 231
SlaI CTCGAG 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 2 cut(s) 11, 137
SsiI CCGC 1 cut(s) 60
SspDI GGCGCC 1 cut(s) 56
SstI GAGCTC 3 cut(s) 180, 228, 297
StyD4I CCNGG 3 cut(s) 99, 198, 207
TaaI ACNGT 2 cut(s) 73, 87
TaiI ACGT 1 cut(s) 147
TaqI TCGA 6 cut(s) 141, 243, 249, 273, 312, 321
TasI AATT 2 cut(s) 11, 137
TauI GCSGC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TseFI GTSAC 1 cut(s) 81
Tsp45I GTSAC 1 cut(s) 81
TspGWI ACGGA 1 cut(s) 307
Tth111I GACNNNGTC 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 231
XapI RAATTY 2 cut(s) 11, 137
XhoI CTCGAG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.