RchiOBHm_Chr7g0240981

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003291
Physical Location & Seq
Forward (+)
66981106 .. 66981625
520 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21598

Sequence Viewer

Length: 474 bp
ATGGCTCAGTCGAGTTCTCTCTCTGCAGAAACCGAGTCATTAAGTCAGGTCTTGGGCCTTATCGAAACATTCAAAGCTTTCGATTCAGACAACGATGGGAAAATCACAGCAGCAGAGCTCGGTGGGATCCTAGGATCGCTTGGTTACAATCCGCTCGAGCAAGATGTGAGGGCAATGATGGAGCAAGGGGACACAAACAAAGATGGGTTGCTGAGCATAGAGGAGTTTCTGGAGATGAACACAAGGAACATGGAATTCGGGGGGCTCGAAAATGTGCTCAAGAATGCTTTTGAGGCCTTGGATGTTGATGTGGATGAGGCTGTGACTGCAGAGGAGTTGTATGAGGTTATGGGAGATGGGATGGGATCTGAGTTGAGCCTGGAGGATTGCCAGGGTATTATTGCTTCTATAGATACAGATGGGGATGGGGCGGTCAGTTTTGAGGACTTCAAACTTATAGTTAACTCCCTCTAA

Protein Analysis

157

Amino Acids

16.82

Weight (kDa)

4.05

Isoelectric Point (pI)

29.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 20 - 79 1.6e-13 EF-hand domain pair
EF-hand_6 PF13405 20 - 48 1.1e-06 EF-hand domain
EF-hand_1 PF00036 21 - 45 9.4e-06 EF hand domain
EF-hand_5 PF13202 21 - 41 8e-06 EF hand
EF-hand_8 PF13833 32 - 82 3.1e-06 EF-hand domain pair
EF-hand_7 PF13499 90 - 151 2e-11 EF-hand domain pair
EF-hand_8 PF13833 108 - 151 4.3e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 154
AciI CCGC 2 cut(s) 152, 431
AclWI GGATC 4 cut(s) 121, 134, 142, 373
AcsI RAATTY 1 cut(s) 254
AgsI TTSAA 2 cut(s) 73, 451
AjnI CCWGG 2 cut(s) 378, 390
AluBI AGCT 2 cut(s) 77, 118
AluI AGCT 2 cut(s) 77, 118
Alw21I GWGCWC 2 cut(s) 120, 279
AlwI GGATC 4 cut(s) 121, 134, 142, 373
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 2 cut(s) 55, 294
ApeKI GCWGC 1 cut(s) 110
ApoI RAATTY 1 cut(s) 254
AspA2I CCTAGG 1 cut(s) 130
AspS9I GGNCC 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 155
AvrII CCTAGG 1 cut(s) 130
BamHI GGATCC 1 cut(s) 126
BanII GRGCYC 2 cut(s) 120, 267
BarI GAAGNNNNNNTAC 2 cut(s) 388, 420
Bbv12I GWGCWC 2 cut(s) 120, 279
BbvI GCAGC 1 cut(s) 122
BccI CCATC 7 cut(s) 89, 172, 197, 350, 355, 413, 419
BciT130I CCWGG 2 cut(s) 380, 392
BfaI CTAG 1 cut(s) 131
BfmI CTRYAG 3 cut(s) 24, 327, 408
BisI GCNGC 1 cut(s) 111
BlnI CCTAGG 1 cut(s) 130
BlpI GCTNAGC 1 cut(s) 212
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 2 cut(s) 380, 392
BmeT110I CYCGRG 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 2 cut(s) 380, 392
BpmI CTGGAG 2 cut(s) 251, 401
Bpu1102I GCTNAGC 1 cut(s) 212
BpuEI CTTGAG 1 cut(s) 263
BsaJI CCNNGG 3 cut(s) 130, 297, 391
Bse3DI GCAATG 1 cut(s) 180
BseBI CCWGG 2 cut(s) 380, 392
BseDI CCNNGG 3 cut(s) 130, 297, 391
BseGI GGATG 4 cut(s) 307, 319, 366, 430
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 3 cut(s) 20, 203, 360
BseRI GAGGAG 2 cut(s) 236, 347
BseXI GCAGC 1 cut(s) 122
BshFI GGCC 2 cut(s) 57, 296
BsiHKAI GWGCWC 2 cut(s) 120, 279
BsiHKCI CYCGRG 1 cut(s) 155
BslFI GGGAC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 203
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 2 cut(s) 57, 296
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 3 cut(s) 120, 267, 279
Bsp143I GATC 3 cut(s) 126, 134, 365
Bsp1720I GCTNAGC 1 cut(s) 212
BspACI CCGC 2 cut(s) 152, 431
BspANI GGCC 2 cut(s) 57, 296
BspCNI CTCAG 3 cut(s) 19, 204, 361
BspLI GGNNCC 1 cut(s) 128
BspMAI CTGCAG 2 cut(s) 28, 331
BspPI GGATC 4 cut(s) 121, 134, 142, 373
BsrBI CCGCTC 1 cut(s) 154
BsrDI GCAATG 1 cut(s) 180
BssECI CCNNGG 3 cut(s) 130, 297, 391
BssMI GATC 3 cut(s) 126, 134, 365
BssT1I CCWWGG 2 cut(s) 130, 297
Bst2UI CCWGG 2 cut(s) 380, 392
BstDEI CTNAG 3 cut(s) 6, 212, 369
BstF5I GGATG 4 cut(s) 307, 319, 366, 430
BstKTI GATC 3 cut(s) 129, 137, 368
BstMBI GATC 3 cut(s) 126, 134, 365
BstMWI GCNNNNNNNGC 2 cut(s) 293, 326
BstNI CCWGG 2 cut(s) 380, 392
BstSCI CCNGG 2 cut(s) 378, 390
BstSFI CTRYAG 3 cut(s) 24, 327, 408
BstV1I GCAGC 1 cut(s) 122
BstX2I RGATCY 2 cut(s) 126, 365
BstYI RGATCY 2 cut(s) 126, 365
BsuRI GGCC 2 cut(s) 57, 296
BtsCI GGATG 4 cut(s) 307, 319, 366, 430
Cfr13I GGNCC 1 cut(s) 55
CviAII CATG 1 cut(s) 250
CviJI RGCY 8 cut(s) 5, 57, 77, 118, 265, 296, 320, 378
CviKI_1 RGCY 8 cut(s) 5, 57, 77, 118, 265, 296, 320, 378
DdeI CTNAG 3 cut(s) 6, 212, 369
DpnI GATC 3 cut(s) 128, 136, 367
DpnII GATC 3 cut(s) 126, 134, 365
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 130, 297
Eco147I AGGCCT 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 120, 267
Eco53kI GAGCTC 1 cut(s) 118
Eco88I CYCGRG 1 cut(s) 155
EcoICRI GAGCTC 1 cut(s) 118
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 378, 390
EcoT14I CCWWGG 2 cut(s) 130, 297
EcoT38I GRGCYC 2 cut(s) 120, 267
ErhI CCWWGG 2 cut(s) 130, 297
FaeI CATG 1 cut(s) 253
FaiI YATR 6 cut(s) 218, 251, 342, 350, 410, 458
FaqI GGGAC 1 cut(s) 203
FatI CATG 1 cut(s) 249
Fnu4HI GCNGC 1 cut(s) 111
FokI GGATG 4 cut(s) 314, 326, 373, 437
FriOI GRGCYC 2 cut(s) 120, 267
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 1 cut(s) 131
GluI GCNGC 1 cut(s) 111
GsuI CTGGAG 2 cut(s) 251, 401
HaeIII GGCC 2 cut(s) 57, 296
Hin1II CATG 1 cut(s) 253
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 2 cut(s) 35, 83
HpaI GTTAAC 1 cut(s) 463
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 2 cut(s) 88, 370
Hpy188III TCNNGA 2 cut(s) 230, 280
Hpy8I GTNNAC 1 cut(s) 463
HpyCH4V TGCA 2 cut(s) 26, 329
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 326
HpyF3I CTNAG 3 cut(s) 6, 212, 369
Hsp92II CATG 1 cut(s) 253
KspAI GTTAAC 1 cut(s) 463
Kzo9I GATC 3 cut(s) 126, 134, 365
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 6 cut(s) 32, 215, 365, 377, 392, 404
Lsp1109I GCAGC 1 cut(s) 122
MaeI CTAG 1 cut(s) 131
MaeIII GTNAC 2 cut(s) 143, 322
MalI GATC 3 cut(s) 128, 136, 367
MbiI CCGCTC 1 cut(s) 154
MboI GATC 3 cut(s) 126, 134, 365
MflI RGATCY 2 cut(s) 126, 365
MhlI GDGCHC 3 cut(s) 120, 267, 279
MluCI AATT 1 cut(s) 254
MlyI GAGTC 1 cut(s) 44
MnlI CCTC 8 cut(s) 162, 214, 286, 310, 325, 337, 376, 436
MseI TTAA 2 cut(s) 41, 462
MspR9I CCNGG 2 cut(s) 380, 392
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 2 cut(s) 380, 392
MwoI GCNNNNNNNGC 2 cut(s) 293, 326
NdeII GATC 3 cut(s) 126, 134, 365
NlaIII CATG 1 cut(s) 253
NlaIV GGNNCC 1 cut(s) 128
NmuCI GTSAC 1 cut(s) 322
PaeR7I CTCGAG 1 cut(s) 155
PceI AGGCCT 1 cut(s) 296
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 1 cut(s) 289
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 1 cut(s) 112
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 378, 390
PspGI CCWGG 2 cut(s) 378, 390
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 1 cut(s) 55
PspXI VCTCGAGB 1 cut(s) 155
PstI CTGCAG 2 cut(s) 28, 331
PsuI RGATCY 2 cut(s) 126, 365
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 2 cut(s) 41, 462
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 3 cut(s) 126, 134, 365
Sau96I GGNCC 1 cut(s) 55
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 2 cut(s) 380, 392
SduI GDGCHC 3 cut(s) 120, 267, 279
SetI ASST 4 cut(s) 51, 79, 120, 348
SfcI CTRYAG 3 cut(s) 24, 327, 408
Sfr274I CTCGAG 1 cut(s) 155
SlaI CTCGAG 1 cut(s) 155
SmlI CTYRAG 2 cut(s) 155, 278
SmoI CTYRAG 2 cut(s) 155, 278
Sse9I AATT 1 cut(s) 254
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 2 cut(s) 152, 431
SspMI CTAG 1 cut(s) 131
SstI GAGCTC 1 cut(s) 120
StuI AGGCCT 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 378, 390
StyI CCWWGG 2 cut(s) 130, 297
TaqI TCGA 5 cut(s) 11, 63, 81, 156, 267
TasI AATT 1 cut(s) 254
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 2 cut(s) 41, 462
Tru9I TTAA 2 cut(s) 41, 462
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 1 cut(s) 110
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 1 cut(s) 251
XapI RAATTY 1 cut(s) 254
XhoI CTCGAG 1 cut(s) 155
XmaJI CCTAGG 1 cut(s) 130
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.