Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0003291

Physical Location & Seq

Genomic Coordinates Reverse (-)

74084821 .. 74100608

Gene Structure 15788 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG466500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 345 bp

ATGCGGTCACCGTCGTTCAACATGCCGGAGCAGATCGTTGAGCTGGAGCAATTCTTCAAGAAATTCGACATCAACGGCGATGGCAAGATCTCGTCGTCGGAGCTCGGAGCGATCATGGGCAGCCTGGGCCACCCGGCGATGGAGGACGAGCTAGAGTCCATGATCCTCAAGGTCGACGGCGATGGCTTCATCGATTTCAAGGAGTTCATGGAGCTCAACACCAACGGGATCGACTCTGCCGAGGTGTTGCAGAATCTCAAGGACACCTTCTCCGTTTACGACATCGATCGCAACGGCTCGATCTCCGCGGAGGAGGAGCTTTTCAAGTTACTACTATTCCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016049 GO:0021700 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0040007 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046872 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050896 GO:0060560 GO:0071695 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

114

Amino Acids

12.8

Weight (kDa)

4.17

Isoelectric Point (pI)

34.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_1	PF00036	14 - 41	6.7e-08	EF hand domain
EF-hand_6	PF13405	14 - 43	1.3e-07	EF-hand domain
EF-hand_7	PF13499	14 - 72	7.6e-10	EF-hand domain pair
EF-hand_5	PF13202	15 - 39	9.9e-08	EF hand

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003291)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24620
fragaria_vesca	FvH4_3g17660 FvH4_3g19960 FvH4_3g29250 FvH4_4g33650 FvH4_5g39020
malus_domestica	MD12G1080300.v1.1 MD13G1020800.v1.1 MD15G1357800.v1.1 MD16G1019200.v1.1
prunus_persica	Prupe.1G505300_v2.0.a1 Prupe.8G060000_v2.0.a1
pyrus_communis	pycom16g01720
rosa_chinensis	RchiOBHm_Chr4g0443111 RchiOBHm_Chr7g0240981
rosa_laevigata	RLG00000000607 RLG00000022992
rosa_multiflora	Rmu_co8148428.1_g000001 Rmu_co8155060.1_g000001 Rmu_sc0000677.1_g000036 Rmu_sc0003187.1_g000018 Rmu_sc0004091.1_g000001 Rmu_sc0007968.1_g000014 Rmu_sc0008069.1_g000001 Rmu_sc0042435.1_g000001
rosa_roxburghii	Rroxscaffold_3G00219340 Rroxscaffold_5G00336800
rosa_rugosa	Rorug04G0344500 Rorug07G0341200
rosa_samantha	Rh4BG410400 Rh4DG405400 Rh5DG466500 Rh7AG496000
rosa_wichuraiana	Rw4G034380 Rw5G040770 Rw7G041910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	174
AccII	CGCG	1 cut(s)	308
AciI	CCGC	3 cut(s)	4, 306, 308
AclWI	GGATC	2 cut(s)	157, 236
AcsI	RAATTY	1 cut(s)	62
AfiI	CCNNNNNNNGG	1 cut(s)	139
AgsI	TTSAA	4 cut(s)	19, 58, 199, 325
AjnI	CCWGG	1 cut(s)	123
AluBI	AGCT	5 cut(s)	43, 103, 151, 214, 319
AluI	AGCT	5 cut(s)	43, 103, 151, 214, 319
Alw21I	GWGCWC	2 cut(s)	105, 216
AlwI	GGATC	2 cut(s)	157, 236
AoxI	GGCC	1 cut(s)	127
ApeKI	GCWGC	1 cut(s)	120
ApoI	RAATTY	1 cut(s)	62
AspS9I	GGNCC	1 cut(s)	127
AsuC2I	CCSGG	1 cut(s)	134
BanII	GRGCYC	2 cut(s)	105, 216
Bbv12I	GWGCWC	2 cut(s)	105, 216
BbvI	GCAGC	1 cut(s)	132
BccI	CCATC	3 cut(s)	74, 133, 176
BceAI	ACGGC	3 cut(s)	91, 193, 310
BciT130I	CCWGG	1 cut(s)	125
BcnI	CCSGG	1 cut(s)	134
BfaI	CTAG	1 cut(s)	152
BglII	AGATCT	1 cut(s)	87
BisI	GCNGC	1 cut(s)	121
BlsI	GCNGC	1 cut(s)	122
Bme1390I	CCNGG	2 cut(s)	125, 134
BmgT120I	GGNCC	1 cut(s)	127
BmrFI	CCNGG	2 cut(s)	125, 134
BpmI	CTGGAG	1 cut(s)	65
BpuEI	CTTGAG	2 cut(s)	152, 242
BpuMI	CCSGG	1 cut(s)	134
Bsa29I	ATCGAT	2 cut(s)	192, 285
BsaJI	CCNNGG	3 cut(s)	124, 240, 306
BsaXI	ACNNNNNCTCC	2 cut(s)	254, 284
Bsc4I	CCNNNNNNNGG	1 cut(s)	139
BseBI	CCWGG	1 cut(s)	125
BseCI	ATCGAT	2 cut(s)	192, 285
BseDI	CCNNGG	3 cut(s)	124, 240, 306
BseLI	CCNNNNNNNGG	1 cut(s)	139
BseRI	GAGGAG	2 cut(s)	326, 329
BseXI	GCAGC	1 cut(s)	132
Bsh1236I	CGCG	1 cut(s)	308
Bsh1285I	CGRYCG	1 cut(s)	289
BshFI	GGCC	1 cut(s)	129
BshVI	ATCGAT	2 cut(s)	192, 285
BsiEI	CGRYCG	1 cut(s)	289
BsiHKAI	GWGCWC	2 cut(s)	105, 216
BsiSI	CCGG	2 cut(s)	26, 134
BslI	CCNNNNNNNGG	1 cut(s)	139
BsnI	GGCC	1 cut(s)	129
Bsp1286I	GDGCHC	2 cut(s)	105, 216
Bsp143I	GATC	7 cut(s)	33, 87, 111, 162, 228, 286, 300
BspACI	CCGC	3 cut(s)	4, 306, 308
BspANI	GGCC	1 cut(s)	129
BspDI	ATCGAT	2 cut(s)	192, 285
BspFNI	CGCG	1 cut(s)	308
BspPI	GGATC	2 cut(s)	157, 236
BssECI	CCNNGG	3 cut(s)	124, 240, 306
BssMI	GATC	7 cut(s)	33, 87, 111, 162, 228, 286, 300
Bst2UI	CCWGG	1 cut(s)	125
Bst4CI	ACNGT	1 cut(s)	12
BstDSI	CCRYGG	1 cut(s)	306
BstEII	GGTNACC	1 cut(s)	6
BstFNI	CGCG	1 cut(s)	308
BstKTI	GATC	7 cut(s)	36, 90, 114, 165, 231, 289, 303
BstMBI	GATC	7 cut(s)	33, 87, 111, 162, 228, 286, 300
BstMCI	CGRYCG	1 cut(s)	289
BstMWI	GCNNNNNNNGC	1 cut(s)	126
BstNI	CCWGG	1 cut(s)	125
BstNSI	RCATGY	1 cut(s)	25
BstPI	GGTNACC	1 cut(s)	6
BstSCI	CCNGG	2 cut(s)	123, 132
BstUI	CGCG	1 cut(s)	308
BstV1I	GCAGC	1 cut(s)	132
BstX2I	RGATCY	1 cut(s)	87
BstYI	RGATCY	1 cut(s)	87
Bsu15I	ATCGAT	2 cut(s)	192, 285
BsuRI	GGCC	1 cut(s)	129
BsuTUI	ATCGAT	2 cut(s)	192, 285
BtgI	CCRYGG	1 cut(s)	306
BtgZI	GCGATG	3 cut(s)	93, 152, 195
Cfr13I	GGNCC	1 cut(s)	127
Cfr42I	CCGCGG	1 cut(s)	309
ClaI	ATCGAT	2 cut(s)	192, 285
CviAII	CATG	5 cut(s)	22, 115, 160, 208, 342
CviJI	RGCY	9 cut(s)	43, 103, 123, 129, 151, 186, 214, 297, 319
CviKI_1	RGCY	9 cut(s)	43, 103, 123, 129, 151, 186, 214, 297, 319
DpnI	GATC	7 cut(s)	35, 89, 113, 164, 230, 288, 302
DpnII	GATC	7 cut(s)	33, 87, 111, 162, 228, 286, 300
Ecl136II	GAGCTC	2 cut(s)	103, 214
Eco24I	GRGCYC	2 cut(s)	105, 216
Eco53kI	GAGCTC	2 cut(s)	103, 214
Eco91I	GGTNACC	1 cut(s)	6
EcoICRI	GAGCTC	2 cut(s)	103, 214
EcoO65I	GGTNACC	1 cut(s)	6
EcoRII	CCWGG	1 cut(s)	123
EcoT38I	GRGCYC	2 cut(s)	105, 216
FaeI	CATG	5 cut(s)	25, 118, 163, 211, 345
FaiI	YATR	5 cut(s)	23, 116, 161, 209, 343
FalI	AAGNNNNNCTT	2 cut(s)	251, 283
FatI	CATG	5 cut(s)	21, 114, 159, 207, 341
FblI	GTMKAC	1 cut(s)	174
Fnu4HI	GCNGC	1 cut(s)	121
FriOI	GRGCYC	2 cut(s)	105, 216
Fsp4HI	GCNGC	1 cut(s)	121
FspBI	CTAG	1 cut(s)	152
GluI	GCNGC	1 cut(s)	121
GsuI	CTGGAG	1 cut(s)	65
HaeIII	GGCC	1 cut(s)	129
HapII	CCGG	2 cut(s)	26, 134
Hin1II	CATG	5 cut(s)	25, 118, 163, 211, 345
HincII	GTYRAC	1 cut(s)	175
HindII	GTYRAC	1 cut(s)	175
HinfI	GANTC	3 cut(s)	155, 233, 253
HpaII	CCGG	2 cut(s)	26, 134
Hpy166II	GTNNAC	2 cut(s)	175, 277
Hpy188I	TCNGA	2 cut(s)	100, 107
Hpy188III	TCNNGA	1 cut(s)	58
Hpy8I	GTNNAC	2 cut(s)	175, 277
Hpy99I	CGWCG	4 cut(s)	16, 97, 100, 179
HpyAV	CCTTC	1 cut(s)	277
HpyCH4III	ACNGT	1 cut(s)	12
HpyCH4V	TGCA	1 cut(s)	250
HpyF10VI	GCNNNNNNNGC	1 cut(s)	126
Hsp92II	CATG	5 cut(s)	25, 118, 163, 211, 345
KspI	CCGCGG	1 cut(s)	309
Kzo9I	GATC	7 cut(s)	33, 87, 111, 162, 228, 286, 300
LmnI	GCTCC	6 cut(s)	28, 46, 100, 107, 211, 316
LpnPI	CCDG	5 cut(s)	29, 39, 110, 137, 147
Lsp1109I	GCAGC	1 cut(s)	132
MaeI	CTAG	1 cut(s)	152
MaeIII	GTNAC	2 cut(s)	6, 327
MalI	GATC	7 cut(s)	35, 89, 113, 164, 230, 288, 302
MboI	GATC	7 cut(s)	33, 87, 111, 162, 228, 286, 300
MboII	GAAGA	1 cut(s)	46
MflI	RGATCY	1 cut(s)	87
MhlI	GDGCHC	2 cut(s)	105, 216
MluCI	AATT	2 cut(s)	50, 62
MlyI	GAGTC	2 cut(s)	164, 227
MmeI	TCCRAC	1 cut(s)	78
MnlI	CCTC	5 cut(s)	136, 176, 235, 304, 307
MspA1I	CMGCKG	1 cut(s)	308
MspI	CCGG	2 cut(s)	26, 134
MspR9I	CCNGG	2 cut(s)	125, 134
MvaI	CCWGG	1 cut(s)	125
MvnI	CGCG	1 cut(s)	308
MwoI	GCNNNNNNNGC	1 cut(s)	126
NciI	CCSGG	1 cut(s)	134
NdeII	GATC	7 cut(s)	33, 87, 111, 162, 228, 286, 300
NlaIII	CATG	5 cut(s)	25, 118, 163, 211, 345
NmeAIII	GCCGAG	1 cut(s)	265
NmuCI	GTSAC	1 cut(s)	6
NspI	RCATGY	1 cut(s)	25
PcsI	WCGNNNNNNNCGW	1 cut(s)	237
PfeI	GAWTC	1 cut(s)	253
PkrI	GCNGC	1 cut(s)	122
Ple19I	CGATCG	1 cut(s)	289
PleI	GAGTC	2 cut(s)	163, 227
PpsI	GAGTC	2 cut(s)	163, 227
Psp124BI	GAGCTC	2 cut(s)	105, 216
Psp6I	CCWGG	1 cut(s)	123
PspEI	GGTNACC	1 cut(s)	6
PspGI	CCWGG	1 cut(s)	123
PspPI	GGNCC	1 cut(s)	127
PsuI	RGATCY	1 cut(s)	87
PvuI	CGATCG	1 cut(s)	289
SacI	GAGCTC	2 cut(s)	105, 216
SacII	CCGCGG	1 cut(s)	309
SalI	GTCGAC	1 cut(s)	173
SatI	GCNGC	1 cut(s)	121
Sau3AI	GATC	7 cut(s)	33, 87, 111, 162, 228, 286, 300
Sau96I	GGNCC	1 cut(s)	127
SchI	GAGTC	2 cut(s)	164, 227
ScrFI	CCNGG	2 cut(s)	125, 134
SduI	GDGCHC	2 cut(s)	105, 216
SetI	ASST	8 cut(s)	45, 105, 153, 174, 216, 246, 269, 321
Sfr303I	CCGCGG	1 cut(s)	309
SgrBI	CCGCGG	1 cut(s)	309
SmlI	CTYRAG	2 cut(s)	167, 257
SmoI	CTYRAG	2 cut(s)	167, 257
Sse9I	AATT	2 cut(s)	50, 62
SsiI	CCGC	3 cut(s)	4, 306, 308
SspMI	CTAG	1 cut(s)	152
SstI	GAGCTC	2 cut(s)	105, 216
StyD4I	CCNGG	2 cut(s)	123, 132
TaaI	ACNGT	1 cut(s)	12
TaqI	TCGA	6 cut(s)	66, 174, 192, 231, 285, 299
TasI	AATT	2 cut(s)	50, 62
TfiI	GAWTC	1 cut(s)	253
TseFI	GTSAC	1 cut(s)	6
TseI	GCWGC	1 cut(s)	120
Tsp45I	GTSAC	1 cut(s)	6
TspDTI	ATGAA	2 cut(s)	178, 196
TspGWI	ACGGA	1 cut(s)	262
XapI	RAATTY	1 cut(s)	62
XceI	RCATGY	1 cut(s)	25
XmiI	GTMKAC	1 cut(s)	174
XspI	CTAG	1 cut(s)	152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.