Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003291

Physical Location & Seq

Genomic Coordinates Forward (+)

1404574 .. 1405182

Gene Structure 609 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1019200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 609 bp

ATGGGTTTCAAATCCCTCTTCTCCCGGAAGAAGAAATCCAGTCCCGACGTCGCCGCTGCCCACCCCGACGTCGCCGCTACCCACCCCGCCGCCTCCGCTGCCCACCCCGCCGCCGCTTCAGCCCACTCGCGAACCCCCTCCTTCCATTCGCGCGCCCAGATTGCCGAGCTCGACCAAGTCTTCAAGAAATTCGATGTCAATGGCGACGGAAAGATCTCGTCTTCCGAGCTCGGAGCCATCATGAGCAGCCTGGGGCAGCCGTCCTCCGACGAGGAGCTCAACAGCATGATCTCCGAGGTCGATTCCGACGGCGACGGCTTCATCAACTTCAAAGAGTTCGTTGAGCTCAACACCAAAGGGGTCGACTCCGCCGAGGCCCTCGAAAATCTCAAAGACGCTTTCTCCGTCTACGACATTGACGGGAACGGGTCTATTTCGGCGGAGGAATTGTACGAAGTGCTCCGGAGCTTGGGAGATGAATGTTCGATCGACGAGTGCCGGCAGATGATCGGCGGCGTCGACAGTGACGGCGATGGTATGATCAGTTTCGAGGAGTTTAAGGTCATGATGATGACGGGATCGCGTTACATTGATCCGGCTTCAGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016049 GO:0021700 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0040007 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046872 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050896 GO:0060560 GO:0071695 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

203

Amino Acids

21.52

Weight (kDa)

4.47

Isoelectric Point (pI)

33.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_1	PF00036	56 - 84	1.8e-08	EF hand domain
EF-hand_7	PF13499	56 - 116	7e-16	EF-hand domain pair
EF-hand_6	PF13405	56 - 85	1.3e-07	EF-hand domain
EF-hand_5	PF13202	57 - 81	5e-08	EF hand
EF-hand_8	PF13833	69 - 116	3.7e-09	EF-hand domain pair
EF-hand_1	PF00036	92 - 116	5.1e-07	EF hand domain
EF-hand_7	PF13499	128 - 190	3.4e-14	EF-hand domain pair
EF-hand_1	PF00036	130 - 157	1.8e-06	EF hand domain
EF-hand_6	PF13405	130 - 158	5.2e-08	EF-hand domain
EF-hand_8	PF13833	142 - 190	7.1e-10	EF-hand domain pair
EF-hand_1	PF00036	165 - 192	9.7e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003291)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24620
fragaria_vesca	FvH4_3g17660 FvH4_3g19960 FvH4_3g29250 FvH4_4g33650 FvH4_5g39020
malus_domestica	MD12G1080300.v1.1 MD13G1020800.v1.1 MD15G1357800.v1.1 MD16G1019200.v1.1
prunus_persica	Prupe.1G505300_v2.0.a1 Prupe.8G060000_v2.0.a1
pyrus_communis	pycom16g01720
rosa_chinensis	RchiOBHm_Chr4g0443111 RchiOBHm_Chr7g0240981
rosa_laevigata	RLG00000000607 RLG00000022992
rosa_multiflora	Rmu_co8148428.1_g000001 Rmu_co8155060.1_g000001 Rmu_sc0000677.1_g000036 Rmu_sc0003187.1_g000018 Rmu_sc0004091.1_g000001 Rmu_sc0007968.1_g000014 Rmu_sc0008069.1_g000001 Rmu_sc0042435.1_g000001
rosa_roxburghii	Rroxscaffold_3G00219340 Rroxscaffold_5G00336800
rosa_rugosa	Rorug04G0344500 Rorug07G0341200
rosa_samantha	Rh4BG410400 Rh4DG405400 Rh5DG466500 Rh7AG496000
rosa_wichuraiana	Rw4G034380 Rw5G040770 Rw7G041910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	51, 72
AccI	GTMKAC	3 cut(s)	363, 408, 519
AccII	CGCG	4 cut(s)	130, 151, 153, 583
AccIII	TCCGGA	1 cut(s)	462
AclWI	GGATC	2 cut(s)	586, 587
AcsI	RAATTY	1 cut(s)	188
AcuI	CTGAAG	2 cut(s)	102, 585
AcyI	GRCGYC	3 cut(s)	48, 69, 516
AfaI	GTAC	1 cut(s)	452
AfiI	CCNNNNNNNGG	2 cut(s)	469, 602
AgsI	TTSAA	3 cut(s)	10, 184, 331
AjnI	CCWGG	1 cut(s)	249
AluBI	AGCT	5 cut(s)	169, 229, 277, 346, 468
AluI	AGCT	5 cut(s)	169, 229, 277, 346, 468
Alw21I	GWGCWC	5 cut(s)	171, 231, 279, 348, 462
AlwI	GGATC	2 cut(s)	586, 587
Aor13HI	TCCGGA	1 cut(s)	462
AoxI	GGCC	1 cut(s)	375
ApeKI	GCWGC	4 cut(s)	56, 98, 246, 256
ApoI	RAATTY	1 cut(s)	188
AspLEI	GCGC	2 cut(s)	153, 155
AspS9I	GGNCC	1 cut(s)	376
AsuC2I	CCSGG	1 cut(s)	25
BanII	GRGCYC	4 cut(s)	171, 231, 279, 348
BbsI	GAAGAC	2 cut(s)	172, 213
Bbv12I	GWGCWC	5 cut(s)	171, 231, 279, 348, 462
BbvI	GCAGC	4 cut(s)	43, 85, 258, 268
BccI	CCATC	2 cut(s)	245, 527
BceAI	ACGGC	4 cut(s)	244, 325, 331, 544
BciT130I	CCWGG	1 cut(s)	251
BclI	TGATCA	1 cut(s)	540
BcnI	CCSGG	1 cut(s)	25
BglII	AGATCT	1 cut(s)	213
Bme1390I	CCNGG	2 cut(s)	25, 251
BmgT120I	GGNCC	1 cut(s)	376
BmiI	GGNNCC	1 cut(s)	235
BmrFI	CCNGG	2 cut(s)	25, 251
BpiI	GAAGAC	2 cut(s)	172, 213
BpuMI	CCSGG	1 cut(s)	25
BsaHI	GRCGYC	3 cut(s)	48, 69, 516
BsaJI	CCNNGG	3 cut(s)	250, 294, 372
BsaWI	WCCGGW	1 cut(s)	462
BsaXI	ACNNNNNCTCC	4 cut(s)	386, 416, 434, 464
Bsc4I	CCNNNNNNNGG	2 cut(s)	469, 602
Bse118I	RCCGGY	1 cut(s)	498
Bse1I	ACTGG	1 cut(s)	39
BseAI	TCCGGA	1 cut(s)	462
BseBI	CCWGG	1 cut(s)	251
BseDI	CCNNGG	3 cut(s)	250, 294, 372
BseLI	CCNNNNNNNGG	2 cut(s)	469, 602
BseNI	ACTGG	1 cut(s)	39
BsePI	GCGCGC	1 cut(s)	151
BseRI	GAGGAG	2 cut(s)	287, 566
BseXI	GCAGC	4 cut(s)	43, 85, 258, 268
Bsh1236I	CGCG	4 cut(s)	130, 151, 153, 583
Bsh1285I	CGRYCG	1 cut(s)	489
BshFI	GGCC	1 cut(s)	377
BsiEI	CGRYCG	1 cut(s)	489
BsiHKAI	GWGCWC	5 cut(s)	171, 231, 279, 348, 462
BsiSI	CCGG	4 cut(s)	25, 463, 499, 596
BslFI	GGGAC	1 cut(s)	27
BslI	CCNNNNNNNGG	2 cut(s)	469, 602
BsmFI	GGGAC	1 cut(s)	27
BsnI	GGCC	1 cut(s)	377
Bsp1286I	GDGCHC	5 cut(s)	171, 231, 279, 348, 462
Bsp13I	TCCGGA	1 cut(s)	462
Bsp143I	GATC	7 cut(s)	213, 288, 486, 507, 540, 578, 592
Bsp68I	TCGCGA	1 cut(s)	130
BspANI	GGCC	1 cut(s)	377
BspEI	TCCGGA	1 cut(s)	462
BspFNI	CGCG	4 cut(s)	130, 151, 153, 583
BspHI	TCATGA	2 cut(s)	240, 564
BspLI	GGNNCC	1 cut(s)	235
BspPI	GGATC	2 cut(s)	586, 587
BsrFI	RCCGGY	1 cut(s)	498
BsrI	ACTGG	1 cut(s)	39
BssAI	RCCGGY	1 cut(s)	498
BssECI	CCNNGG	3 cut(s)	250, 294, 372
BssHII	GCGCGC	1 cut(s)	151
BssMI	GATC	7 cut(s)	213, 288, 486, 507, 540, 578, 592
BssNI	GRCGYC	3 cut(s)	48, 69, 516
Bst2UI	CCWGG	1 cut(s)	251
Bst4CI	ACNGT	1 cut(s)	524
Bst6I	CTCTTC	1 cut(s)	23
BstACI	GRCGYC	3 cut(s)	48, 69, 516
BstC8I	GCNNGC	2 cut(s)	153, 500
BstFNI	CGCG	4 cut(s)	130, 151, 153, 583
BstHHI	GCGC	2 cut(s)	153, 155
BstKTI	GATC	7 cut(s)	216, 291, 489, 510, 543, 581, 595
BstMBI	GATC	7 cut(s)	213, 288, 486, 507, 540, 578, 592
BstMCI	CGRYCG	1 cut(s)	489
BstMWI	GCNNNNNNNGC	5 cut(s)	95, 98, 107, 119, 161
BstNI	CCWGG	1 cut(s)	251
BstSCI	CCNGG	2 cut(s)	23, 249
BstUI	CGCG	4 cut(s)	130, 151, 153, 583
BstV1I	GCAGC	4 cut(s)	43, 85, 258, 268
BstV2I	GAAGAC	2 cut(s)	172, 213
BstX2I	RGATCY	1 cut(s)	213
BstYI	RGATCY	1 cut(s)	213
BsuRI	GGCC	1 cut(s)	377
BtgZI	GCGATG	1 cut(s)	546
BtsIMutI	CAGTG	1 cut(s)	529
BtuMI	TCGCGA	1 cut(s)	130
Cac8I	GCNNGC	2 cut(s)	153, 500
CciI	TCATGA	2 cut(s)	240, 564
CfoI	GCGC	2 cut(s)	153, 155
Cfr10I	RCCGGY	1 cut(s)	498
Cfr13I	GGNCC	1 cut(s)	376
CseI	GACGC	2 cut(s)	404, 505
Csp6I	GTAC	1 cut(s)	451
CviAII	CATG	3 cut(s)	241, 286, 565
CviQI	GTAC	1 cut(s)	451
DpnI	GATC	7 cut(s)	215, 290, 488, 509, 542, 580, 594
DpnII	GATC	7 cut(s)	213, 288, 486, 507, 540, 578, 592
Eam1104I	CTCTTC	1 cut(s)	23
EarI	CTCTTC	1 cut(s)	23
EciI	GGCGGA	2 cut(s)	358, 455
Ecl136II	GAGCTC	4 cut(s)	169, 229, 277, 346
Eco24I	GRGCYC	4 cut(s)	171, 231, 279, 348
Eco53kI	GAGCTC	4 cut(s)	169, 229, 277, 346
Eco57I	CTGAAG	2 cut(s)	102, 585
EcoICRI	GAGCTC	4 cut(s)	169, 229, 277, 346
EcoO109I	RGGNCCY	1 cut(s)	376
EcoRII	CCWGG	1 cut(s)	249
EcoT38I	GRGCYC	4 cut(s)	171, 231, 279, 348
FaeI	CATG	3 cut(s)	244, 289, 568
FaiI	YATR	4 cut(s)	242, 287, 539, 566
FaqI	GGGAC	1 cut(s)	27
FatI	CATG	3 cut(s)	240, 285, 564
FauI	CCCGC	2 cut(s)	94, 115
FbaI	TGATCA	1 cut(s)	540
FblI	GTMKAC	3 cut(s)	363, 408, 519
FriOI	GRGCYC	4 cut(s)	171, 231, 279, 348
GlaI	GCGC	2 cut(s)	152, 154
HaeIII	GGCC	1 cut(s)	377
HapII	CCGG	4 cut(s)	25, 463, 499, 596
HgaI	GACGC	2 cut(s)	404, 505
HhaI	GCGC	2 cut(s)	153, 155
Hin1I	GRCGYC	3 cut(s)	48, 69, 516
Hin1II	CATG	3 cut(s)	244, 289, 568
Hin6I	GCGC	2 cut(s)	151, 153
HinP1I	GCGC	2 cut(s)	151, 153
HincII	GTYRAC	2 cut(s)	364, 520
HindII	GTYRAC	2 cut(s)	364, 520
HinfI	GANTC	2 cut(s)	302, 365
HpaII	CCGG	4 cut(s)	25, 463, 499, 596
Hpy166II	GTNNAC	3 cut(s)	364, 409, 520
Hpy188I	TCNGA	5 cut(s)	226, 233, 268, 295, 307
Hpy188III	TCNNGA	6 cut(s)	44, 129, 184, 241, 463, 565
Hpy8I	GTNNAC	3 cut(s)	364, 409, 520
HpyAV	CCTTC	1 cut(s)	151
HpyCH4III	ACNGT	1 cut(s)	524
HpyCH4IV	ACGT	2 cut(s)	48, 69
HpyF10VI	GCNNNNNNNGC	5 cut(s)	95, 98, 107, 119, 161
HpySE526I	ACGT	2 cut(s)	48, 69
Hsp92I	GRCGYC	3 cut(s)	48, 69, 516
Hsp92II	CATG	3 cut(s)	244, 289, 568
HspAI	GCGC	2 cut(s)	151, 153
Kpn2I	TCCGGA	1 cut(s)	462
KroI	GCCGGC	1 cut(s)	498
KroNI	GCCGGC	1 cut(s)	500
Ksp22I	TGATCA	1 cut(s)	540
Kzo9I	GATC	7 cut(s)	213, 288, 486, 507, 540, 578, 592
LmnI	GCTCC	4 cut(s)	233, 274, 465, 465
LpnPI	CCDG	8 cut(s)	38, 52, 170, 236, 263, 476, 512, 588
Lsp1109I	GCAGC	4 cut(s)	43, 85, 258, 268
MaeII	ACGT	2 cut(s)	48, 69
MaeIII	GTNAC	2 cut(s)	524, 584
MalI	GATC	7 cut(s)	215, 290, 488, 509, 542, 580, 594
MboI	GATC	7 cut(s)	213, 288, 486, 507, 540, 578, 592
MboII	GAAGA	5 cut(s)	10, 40, 43, 172, 213
MflI	RGATCY	1 cut(s)	213
MhlI	GDGCHC	5 cut(s)	171, 231, 279, 348, 462
MluCI	AATT	2 cut(s)	188, 446
MlyI	GAGTC	1 cut(s)	359
MmeI	TCCRAC	2 cut(s)	291, 330
MroI	TCCGGA	1 cut(s)	462
MroNI	GCCGGC	1 cut(s)	498
MseI	TTAA	1 cut(s)	558
MslI	CAYNNNNRTG	1 cut(s)	569
MspA1I	CMGCKG	2 cut(s)	56, 98
MspI	CCGG	4 cut(s)	25, 463, 499, 596
MspR9I	CCNGG	2 cut(s)	25, 251
MvaI	CCWGG	1 cut(s)	251
MvnI	CGCG	4 cut(s)	130, 151, 153, 583
MwoI	GCNNNNNNNGC	5 cut(s)	95, 98, 107, 119, 161
NaeI	GCCGGC	1 cut(s)	500
NciI	CCSGG	1 cut(s)	25
NdeII	GATC	7 cut(s)	213, 288, 486, 507, 540, 578, 592
NgoMIV	GCCGGC	1 cut(s)	498
NlaIII	CATG	3 cut(s)	244, 289, 568
NlaIV	GGNNCC	1 cut(s)	235
NmeAIII	GCCGAG	2 cut(s)	190, 397
NmuCI	GTSAC	1 cut(s)	524
NruI	TCGCGA	1 cut(s)	130
PagI	TCATGA	2 cut(s)	240, 564
PauI	GCGCGC	1 cut(s)	151
PcsI	WCGNNNNNNNCGW	3 cut(s)	369, 402, 516
PdiI	GCCGGC	1 cut(s)	500
PfeI	GAWTC	1 cut(s)	302
PflFI	GACNNNGTC	1 cut(s)	176
PfoI	TCCNGGA	1 cut(s)	23
Ple19I	CGATCG	1 cut(s)	489
PleI	GAGTC	1 cut(s)	359
PpsI	GAGTC	1 cut(s)	359
Psp124BI	GAGCTC	4 cut(s)	171, 231, 279, 348
Psp6I	CCWGG	1 cut(s)	249
PspGI	CCWGG	1 cut(s)	249
PspN4I	GGNNCC	1 cut(s)	235
PspPI	GGNCC	1 cut(s)	376
PsuI	RGATCY	1 cut(s)	213
PsyI	GACNNNGTC	1 cut(s)	176
PteI	GCGCGC	1 cut(s)	151
PvuI	CGATCG	1 cut(s)	489
RruI	TCGCGA	1 cut(s)	130
RsaI	GTAC	1 cut(s)	452
RsaNI	GTAC	1 cut(s)	451
RseI	CAYNNNNRTG	1 cut(s)	569
SacI	GAGCTC	4 cut(s)	171, 231, 279, 348
SalI	GTCGAC	2 cut(s)	362, 518
SaqAI	TTAA	1 cut(s)	558
Sau3AI	GATC	7 cut(s)	213, 288, 486, 507, 540, 578, 592
Sau96I	GGNCC	1 cut(s)	376
SchI	GAGTC	1 cut(s)	359
ScrFI	CCNGG	2 cut(s)	25, 251
SduI	GDGCHC	5 cut(s)	171, 231, 279, 348, 462
SmiMI	CAYNNNNRTG	1 cut(s)	569
Sse9I	AATT	2 cut(s)	188, 446
SstI	GAGCTC	4 cut(s)	171, 231, 279, 348
StyD4I	CCNGG	2 cut(s)	23, 249
TaaI	ACNGT	1 cut(s)	524
TaiI	ACGT	2 cut(s)	51, 72
TaqI	TCGA	9 cut(s)	171, 192, 300, 363, 381, 485, 489, 519, 549
TasI	AATT	2 cut(s)	188, 446
TauI	GCSGC	6 cut(s)	56, 77, 92, 113, 116, 516
TfiI	GAWTC	1 cut(s)	302
Tru1I	TTAA	1 cut(s)	558
Tru9I	TTAA	1 cut(s)	558
TscAI	CASTG	1 cut(s)	529
TseFI	GTSAC	1 cut(s)	524
TseI	GCWGC	4 cut(s)	56, 98, 246, 256
Tsp45I	GTSAC	1 cut(s)	524
TspDTI	ATGAA	2 cut(s)	310, 492
TspGWI	ACGGA	2 cut(s)	222, 394
TspRI	CASTG	1 cut(s)	529
Tth111I	GACNNNGTC	1 cut(s)	176
XapI	RAATTY	1 cut(s)	188
XmiI	GTMKAC	3 cut(s)	363, 408, 519
ZraI	GACGTC	2 cut(s)	49, 70

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.