Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003291

Physical Location & Seq

Genomic Coordinates Forward (+)

1304316 .. 1304989

Gene Structure 674 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1020800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 582 bp

ATGGGTTTCAAATCCCTCTTCTCCCGGAAGAAGAAATCCAGTCCCTCCTCCGCCGCCGCCGCCGACGCACCCCACCCCGCCTTCGCCCCCACGCACTCGCGATCCCCCTCCTTCCACTCGCGCGCCCAGATCGCCGAGCTCGACCAGGTCTTCAAGAAATTTGACGTCAATGGCGACGGCAAGATCTCGTCGTCCGAGCTCGGAGCAATCATGAGCAGCCTGGGGCAGCCGGCCTCCGACGAGGAGCTCAACGGCATGATCTCCGAGGTCGATTCCGACGGCGACGGCTTCATCAACTTCAAAGAGTTCGTTGAGCTCAACACCAAAGGGATCGACCCGGACGAGGCGCTCGAGAATCTCAAGGACGCCTTCTCCGTCTACGACATTGACGGGAACGGCTCGATTTCGGTGGAGGAATTGTACAAAGTTCTCCGGAGCTTGGGGGACGAATGTTCGATGGACGAGTGCCGGAAGATGATCGGTGGCGTCGACAGTGACGGCGACGGTATGATCAATTTCGAGGAGTTTAAGGTCATGATGATGACGGGATCGCGTTACACTGATCCGGTTTCAGTTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016049 GO:0021700 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0040007 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046872 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050896 GO:0060560 GO:0071695 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

194

Amino Acids

20.9

Weight (kDa)

4.5

Isoelectric Point (pI)

40.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_1	PF00036	46 - 74	1.7e-08	EF hand domain
EF-hand_7	PF13499	46 - 106	4.6e-15	EF-hand domain pair
EF-hand_6	PF13405	46 - 75	1.2e-07	EF-hand domain
EF-hand_5	PF13202	47 - 71	4.7e-08	EF hand
EF-hand_8	PF13833	59 - 106	1.4e-08	EF-hand domain pair
EF-hand_1	PF00036	82 - 106	1.5e-06	EF hand domain
AIF-1	PF21008	114 - 185	7.7e-06	Allograft inflammatory factor 1
EF-hand_7	PF13499	118 - 180	8.7e-15	EF-hand domain pair
EF-hand_1	PF00036	120 - 147	2.1e-06	EF hand domain
EF-hand_6	PF13405	120 - 148	3.1e-08	EF-hand domain
EF-hand_5	PF13202	120 - 143	8e-06	EF hand
EF-hand_8	PF13833	132 - 180	4.8e-10	EF-hand domain pair
EF-hand_1	PF00036	155 - 182	8.4e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003291)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24620
fragaria_vesca	FvH4_3g17660 FvH4_3g19960 FvH4_3g29250 FvH4_4g33650 FvH4_5g39020
malus_domestica	MD12G1080300.v1.1 MD13G1020800.v1.1 MD15G1357800.v1.1 MD16G1019200.v1.1
prunus_persica	Prupe.1G505300_v2.0.a1 Prupe.8G060000_v2.0.a1
pyrus_communis	pycom16g01720
rosa_chinensis	RchiOBHm_Chr4g0443111 RchiOBHm_Chr7g0240981
rosa_laevigata	RLG00000000607 RLG00000022992
rosa_multiflora	Rmu_co8148428.1_g000001 Rmu_co8155060.1_g000001 Rmu_sc0000677.1_g000036 Rmu_sc0003187.1_g000018 Rmu_sc0004091.1_g000001 Rmu_sc0007968.1_g000014 Rmu_sc0008069.1_g000001 Rmu_sc0042435.1_g000001
rosa_roxburghii	Rroxscaffold_3G00219340 Rroxscaffold_5G00336800
rosa_rugosa	Rorug04G0344500 Rorug07G0341200
rosa_samantha	Rh4BG410400 Rh4DG405400 Rh5DG466500 Rh7AG496000
rosa_wichuraiana	Rw4G034380 Rw5G040770 Rw7G041910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	168
AccI	GTMKAC	2 cut(s)	378, 489
AccII	CGCG	4 cut(s)	100, 121, 123, 553
AccIII	TCCGGA	1 cut(s)	432
AciI	CCGC	5 cut(s)	51, 54, 57, 60, 78
AclWI	GGATC	4 cut(s)	96, 338, 556, 557
AcsI	RAATTY	1 cut(s)	158
AcyI	GRCGYC	3 cut(s)	165, 366, 486
AfaI	GTAC	1 cut(s)	422
AfiI	CCNNNNNNNGG	2 cut(s)	343, 439
AgsI	TTSAA	3 cut(s)	10, 154, 301
AjnI	CCWGG	2 cut(s)	144, 219
AluBI	AGCT	5 cut(s)	139, 199, 247, 316, 438
AluI	AGCT	5 cut(s)	139, 199, 247, 316, 438
Alw21I	GWGCWC	4 cut(s)	141, 201, 249, 318
AlwI	GGATC	4 cut(s)	96, 338, 556, 557
Ama87I	CYCGRG	1 cut(s)	350
Aor13HI	TCCGGA	1 cut(s)	432
AoxI	GGCC	1 cut(s)	231
ApeKI	GCWGC	2 cut(s)	216, 226
ApoI	RAATTY	1 cut(s)	158
AspLEI	GCGC	3 cut(s)	123, 125, 349
AsuC2I	CCSGG	2 cut(s)	25, 338
AvaI	CYCGRG	1 cut(s)	350
BanII	GRGCYC	4 cut(s)	141, 201, 249, 318
BbsI	GAAGAC	1 cut(s)	142
Bbv12I	GWGCWC	4 cut(s)	141, 201, 249, 318
BbvI	GCAGC	2 cut(s)	228, 238
BccI	CCATC	1 cut(s)	451
BceAI	ACGGC	6 cut(s)	193, 268, 295, 301, 412, 514
BciT130I	CCWGG	2 cut(s)	146, 221
BclI	TGATCA	1 cut(s)	510
BcnI	CCSGG	2 cut(s)	25, 338
BfoI	RGCGCY	1 cut(s)	350
BglII	AGATCT	1 cut(s)	183
BisI	GCNGC	5 cut(s)	54, 57, 60, 217, 227
BlsI	GCNGC	5 cut(s)	55, 58, 61, 218, 228
Bme1390I	CCNGG	4 cut(s)	25, 146, 221, 338
BmeT110I	CYCGRG	1 cut(s)	350
BmrFI	CCNGG	4 cut(s)	25, 146, 221, 338
BpiI	GAAGAC	1 cut(s)	142
BpuEI	CTTGAG	1 cut(s)	344
BpuMI	CCSGG	2 cut(s)	25, 338
BsaHI	GRCGYC	3 cut(s)	165, 366, 486
BsaJI	CCNNGG	2 cut(s)	220, 264
BsaWI	WCCGGW	2 cut(s)	432, 565
BsaXI	ACNNNNNCTCC	4 cut(s)	356, 386, 404, 434
Bsc4I	CCNNNNNNNGG	2 cut(s)	343, 439
Bse118I	RCCGGY	1 cut(s)	229
Bse1I	ACTGG	1 cut(s)	39
BseAI	TCCGGA	1 cut(s)	432
BseBI	CCWGG	2 cut(s)	146, 221
BseDI	CCNNGG	2 cut(s)	220, 264
BseLI	CCNNNNNNNGG	2 cut(s)	343, 439
BseNI	ACTGG	1 cut(s)	39
BsePI	GCGCGC	1 cut(s)	121
BseRI	GAGGAG	3 cut(s)	37, 257, 536
BseXI	GCAGC	2 cut(s)	228, 238
Bsh1236I	CGCG	4 cut(s)	100, 121, 123, 553
BshFI	GGCC	1 cut(s)	233
BsiHKAI	GWGCWC	4 cut(s)	141, 201, 249, 318
BsiHKCI	CYCGRG	1 cut(s)	350
BsiSI	CCGG	6 cut(s)	25, 230, 338, 433, 469, 566
BslFI	GGGAC	2 cut(s)	27, 458
BslI	CCNNNNNNNGG	2 cut(s)	343, 439
BsmFI	GGGAC	2 cut(s)	27, 458
BsnI	GGCC	1 cut(s)	233
BsoBI	CYCGRG	1 cut(s)	350
Bsp1286I	GDGCHC	4 cut(s)	141, 201, 249, 318
Bsp13I	TCCGGA	1 cut(s)	432
Bsp1407I	TGTACA	1 cut(s)	420
Bsp143I	GATC	9 cut(s)	101, 129, 183, 258, 330, 477, 510, 548, 562
Bsp68I	TCGCGA	1 cut(s)	100
BspACI	CCGC	5 cut(s)	51, 54, 57, 60, 78
BspANI	GGCC	1 cut(s)	233
BspEI	TCCGGA	1 cut(s)	432
BspFNI	CGCG	4 cut(s)	100, 121, 123, 553
BspHI	TCATGA	2 cut(s)	210, 534
BspPI	GGATC	4 cut(s)	96, 338, 556, 557
BsrFI	RCCGGY	1 cut(s)	229
BsrGI	TGTACA	1 cut(s)	420
BsrI	ACTGG	1 cut(s)	39
BssAI	RCCGGY	1 cut(s)	229
BssECI	CCNNGG	2 cut(s)	220, 264
BssHII	GCGCGC	1 cut(s)	121
BssMI	GATC	9 cut(s)	101, 129, 183, 258, 330, 477, 510, 548, 562
BssNI	GRCGYC	3 cut(s)	165, 366, 486
Bst2UI	CCWGG	2 cut(s)	146, 221
Bst4CI	ACNGT	2 cut(s)	494, 506
Bst6I	CTCTTC	1 cut(s)	23
BstACI	GRCGYC	3 cut(s)	165, 366, 486
BstAUI	TGTACA	1 cut(s)	420
BstC8I	GCNNGC	2 cut(s)	123, 231
BstFNI	CGCG	4 cut(s)	100, 121, 123, 553
BstH2I	RGCGCY	1 cut(s)	350
BstHHI	GCGC	3 cut(s)	123, 125, 349
BstKTI	GATC	9 cut(s)	104, 132, 186, 261, 333, 480, 513, 551, 565
BstMBI	GATC	9 cut(s)	101, 129, 183, 258, 330, 477, 510, 548, 562
BstMWI	GCNNNNNNNGC	3 cut(s)	59, 65, 131
BstNI	CCWGG	2 cut(s)	146, 221
BstSCI	CCNGG	4 cut(s)	23, 144, 219, 336
BstUI	CGCG	4 cut(s)	100, 121, 123, 553
BstV1I	GCAGC	2 cut(s)	228, 238
BstV2I	GAAGAC	1 cut(s)	142
BstX2I	RGATCY	1 cut(s)	183
BstYI	RGATCY	1 cut(s)	183
BsuRI	GGCC	1 cut(s)	233
BtsIMutI	CAGTG	2 cut(s)	499, 558
BtuMI	TCGCGA	1 cut(s)	100
Cac8I	GCNNGC	2 cut(s)	123, 231
CciI	TCATGA	2 cut(s)	210, 534
CfoI	GCGC	3 cut(s)	123, 125, 349
Cfr10I	RCCGGY	1 cut(s)	229
CseI	GACGC	3 cut(s)	74, 374, 475
CsiI	ACCWGGT	1 cut(s)	144
Csp6I	GTAC	1 cut(s)	421
CviAII	CATG	3 cut(s)	211, 256, 535
CviQI	GTAC	1 cut(s)	421
DpnI	GATC	9 cut(s)	103, 131, 185, 260, 332, 479, 512, 550, 564
DpnII	GATC	9 cut(s)	101, 129, 183, 258, 330, 477, 510, 548, 562
Eam1104I	CTCTTC	1 cut(s)	23
EarI	CTCTTC	1 cut(s)	23
EciI	GGCGGA	1 cut(s)	40
Ecl136II	GAGCTC	4 cut(s)	139, 199, 247, 316
Eco24I	GRGCYC	4 cut(s)	141, 201, 249, 318
Eco53kI	GAGCTC	4 cut(s)	139, 199, 247, 316
Eco88I	CYCGRG	1 cut(s)	350
EcoICRI	GAGCTC	4 cut(s)	139, 199, 247, 316
EcoRII	CCWGG	2 cut(s)	144, 219
EcoT38I	GRGCYC	4 cut(s)	141, 201, 249, 318
FaeI	CATG	3 cut(s)	214, 259, 538
FaiI	YATR	4 cut(s)	212, 257, 509, 536
FalI	AAGNNNNNCTT	2 cut(s)	353, 385
FaqI	GGGAC	2 cut(s)	27, 458
FatI	CATG	3 cut(s)	210, 255, 534
FauI	CCCGC	1 cut(s)	85
FbaI	TGATCA	1 cut(s)	510
FblI	GTMKAC	2 cut(s)	378, 489
Fnu4HI	GCNGC	5 cut(s)	54, 57, 60, 217, 227
FriOI	GRGCYC	4 cut(s)	141, 201, 249, 318
Fsp4HI	GCNGC	5 cut(s)	54, 57, 60, 217, 227
GlaI	GCGC	3 cut(s)	122, 124, 348
GluI	GCNGC	5 cut(s)	54, 57, 60, 217, 227
HaeII	RGCGCY	1 cut(s)	350
HaeIII	GGCC	1 cut(s)	233
HapII	CCGG	6 cut(s)	25, 230, 338, 433, 469, 566
HgaI	GACGC	3 cut(s)	74, 374, 475
HhaI	GCGC	3 cut(s)	123, 125, 349
Hin1I	GRCGYC	3 cut(s)	165, 366, 486
Hin1II	CATG	3 cut(s)	214, 259, 538
Hin6I	GCGC	3 cut(s)	121, 123, 347
HinP1I	GCGC	3 cut(s)	121, 123, 347
HincII	GTYRAC	1 cut(s)	490
HindII	GTYRAC	1 cut(s)	490
HinfI	GANTC	2 cut(s)	272, 355
HpaII	CCGG	6 cut(s)	25, 230, 338, 433, 469, 566
Hpy166II	GTNNAC	2 cut(s)	379, 490
Hpy188I	TCNGA	5 cut(s)	196, 203, 238, 265, 277
Hpy188III	TCNNGA	6 cut(s)	99, 154, 211, 352, 433, 535
Hpy8I	GTNNAC	2 cut(s)	379, 490
Hpy99I	CGWCG	8 cut(s)	68, 179, 193, 242, 281, 287, 491, 506
HpyAV	CCTTC	3 cut(s)	91, 121, 379
HpyCH4III	ACNGT	2 cut(s)	494, 506
HpyCH4IV	ACGT	1 cut(s)	165
HpyF10VI	GCNNNNNNNGC	3 cut(s)	59, 65, 131
HpySE526I	ACGT	1 cut(s)	165
Hsp92I	GRCGYC	3 cut(s)	165, 366, 486
Hsp92II	CATG	3 cut(s)	214, 259, 538
HspAI	GCGC	3 cut(s)	121, 123, 347
Kpn2I	TCCGGA	1 cut(s)	432
KroI	GCCGGC	1 cut(s)	229
KroNI	GCCGGC	1 cut(s)	231
Ksp22I	TGATCA	1 cut(s)	510
Kzo9I	GATC	9 cut(s)	101, 129, 183, 258, 330, 477, 510, 548, 562
LmnI	GCTCC	3 cut(s)	203, 244, 435
Lsp1109I	GCAGC	2 cut(s)	228, 238
MabI	ACCWGGT	1 cut(s)	144
MaeII	ACGT	1 cut(s)	165
MaeIII	GTNAC	2 cut(s)	494, 554
MalI	GATC	9 cut(s)	103, 131, 185, 260, 332, 479, 512, 550, 564
MboI	GATC	9 cut(s)	101, 129, 183, 258, 330, 477, 510, 548, 562
MboII	GAAGA	5 cut(s)	10, 40, 43, 142, 484
MflI	RGATCY	1 cut(s)	183
MhlI	GDGCHC	4 cut(s)	141, 201, 249, 318
MluCI	AATT	4 cut(s)	158, 416, 514, 577
MmeI	TCCRAC	2 cut(s)	261, 300
MroI	TCCGGA	1 cut(s)	432
MroNI	GCCGGC	1 cut(s)	229
MseI	TTAA	3 cut(s)	528, 576, 580
MslI	CAYNNNNRTG	1 cut(s)	539
MspI	CCGG	6 cut(s)	25, 230, 338, 433, 469, 566
MspR9I	CCNGG	4 cut(s)	25, 146, 221, 338
MvaI	CCWGG	2 cut(s)	146, 221
MvnI	CGCG	4 cut(s)	100, 121, 123, 553
MwoI	GCNNNNNNNGC	3 cut(s)	59, 65, 131
NaeI	GCCGGC	1 cut(s)	231
NciI	CCSGG	2 cut(s)	25, 338
NdeII	GATC	9 cut(s)	101, 129, 183, 258, 330, 477, 510, 548, 562
NgoMIV	GCCGGC	1 cut(s)	229
NlaIII	CATG	3 cut(s)	214, 259, 538
NmeAIII	GCCGAG	1 cut(s)	160
NmuCI	GTSAC	1 cut(s)	494
NruI	TCGCGA	1 cut(s)	100
PacI	TTAATTAA	1 cut(s)	580
PaeR7I	CTCGAG	1 cut(s)	350
PagI	TCATGA	2 cut(s)	210, 534
PauI	GCGCGC	1 cut(s)	121
PcsI	WCGNNNNNNNCGW	6 cut(s)	138, 171, 339, 348, 372, 486
PdiI	GCCGGC	1 cut(s)	231
PfeI	GAWTC	2 cut(s)	272, 355
PflFI	GACNNNGTC	1 cut(s)	146
PfoI	TCCNGGA	1 cut(s)	23
PkrI	GCNGC	5 cut(s)	55, 58, 61, 218, 228
Psp124BI	GAGCTC	4 cut(s)	141, 201, 249, 318
Psp6I	CCWGG	2 cut(s)	144, 219
PspGI	CCWGG	2 cut(s)	144, 219
PsuI	RGATCY	1 cut(s)	183
PsyI	GACNNNGTC	1 cut(s)	146
PteI	GCGCGC	1 cut(s)	121
RruI	TCGCGA	1 cut(s)	100
RsaI	GTAC	1 cut(s)	422
RsaNI	GTAC	1 cut(s)	421
RseI	CAYNNNNRTG	1 cut(s)	539
SacI	GAGCTC	4 cut(s)	141, 201, 249, 318
SalI	GTCGAC	1 cut(s)	488
SaqAI	TTAA	3 cut(s)	528, 576, 580
SatI	GCNGC	5 cut(s)	54, 57, 60, 217, 227
Sau3AI	GATC	9 cut(s)	101, 129, 183, 258, 330, 477, 510, 548, 562
ScrFI	CCNGG	4 cut(s)	25, 146, 221, 338
SduI	GDGCHC	4 cut(s)	141, 201, 249, 318
SetI	ASST	9 cut(s)	141, 150, 168, 201, 249, 270, 318, 440, 534
SexAI	ACCWGGT	1 cut(s)	144
Sfr274I	CTCGAG	1 cut(s)	350
SlaI	CTCGAG	1 cut(s)	350
SmiMI	CAYNNNNRTG	1 cut(s)	539
SmlI	CTYRAG	2 cut(s)	350, 359
SmoI	CTYRAG	2 cut(s)	350, 359
Sse9I	AATT	4 cut(s)	158, 416, 514, 577
SsiI	CCGC	5 cut(s)	51, 54, 57, 60, 78
SstI	GAGCTC	4 cut(s)	141, 201, 249, 318
StyD4I	CCNGG	4 cut(s)	23, 144, 219, 336
TaaI	ACNGT	2 cut(s)	494, 506
TaiI	ACGT	1 cut(s)	168
TaqI	TCGA	8 cut(s)	141, 270, 333, 351, 401, 455, 489, 519
TasI	AATT	4 cut(s)	158, 416, 514, 577
TatI	WGTACW	1 cut(s)	420
TauI	GCSGC	3 cut(s)	56, 59, 62
TfiI	GAWTC	2 cut(s)	272, 355
Tru1I	TTAA	3 cut(s)	528, 576, 580
Tru9I	TTAA	3 cut(s)	528, 576, 580
TscAI	CASTG	2 cut(s)	499, 565
TseFI	GTSAC	1 cut(s)	494
TseI	GCWGC	2 cut(s)	216, 226
Tsp45I	GTSAC	1 cut(s)	494
TspDTI	ATGAA	1 cut(s)	280
TspGWI	ACGGA	1 cut(s)	364
TspRI	CASTG	2 cut(s)	499, 565
Tth111I	GACNNNGTC	1 cut(s)	146
XapI	RAATTY	1 cut(s)	158
XhoI	CTCGAG	1 cut(s)	350
XmiI	GTMKAC	2 cut(s)	378, 489
ZraI	GACGTC	1 cut(s)	166

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.