RchiOBHm_Chr4g0443111

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003291
Physical Location & Seq
Forward (+)
64422094 .. 64423122
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41083

Sequence Viewer

Length: 573 bp
ATGGGTTTCAAATCCCTCTTCTCCCGGAAGAAGAAATCCACTCCCTCCAGCGCCGGCGCCGCTCAGCCGGCGGTGATGCGGTCACCGTCGTTCAACACGCGGGCGCAGATCGCCGAGCTGGAGCAGGTCTTCAAGAAATTCGACGTCAACGGCGACGGCAAGATCTCGTCGTCGGAGCTCGGCGCGATCATGGGCAGCCTGGGCCACCCGGCGACGGAGGACGAGCTCCGGTCCATGATCCTCGAGGTCGACGCCGACGGCGACGGCTTCATCGACTTCAAGGAGTTCGTGGAGCTCAACACCAACGGGATCGACTCCGCCGAGGCGTTGCAGAATCTCAAGGACGCCTTCTCCGTCTACGACATCGACGGCAACGGCTCGATCTCCGCGGAGGAGCTTTTCGAGGTGCTGAGGAGCTTGGGCGACGAGTGCTCGATCGGCGAGTGCCGGAAAATGATCAGCGGCGTCGATGCCAACGGCGACGGTATGATCAATTTTGAGGAGTTTAAAGAAATGATGATGATGGGGTCGCGTTATGAGAATAATGCTCAACCTCAACCTGTTGGTAATTGA

Protein Analysis

190

Amino Acids

20.6

Weight (kDa)

4.45

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 39 - 66 6.8e-08 EF hand domain
EF-hand_7 PF13499 39 - 99 6.9e-15 EF-hand domain pair
EF-hand_6 PF13405 39 - 68 9.5e-08 EF-hand domain
EF-hand_5 PF13202 40 - 64 5.1e-08 EF hand
EF-hand_8 PF13833 52 - 99 2.2e-06 EF-hand domain pair
EF-hand_1 PF00036 75 - 99 6.3e-07 EF hand domain
EF-hand_7 PF13499 112 - 174 1.3e-14 EF-hand domain pair
EF-hand_1 PF00036 113 - 140 8.3e-07 EF hand domain
EF-hand_6 PF13405 113 - 141 8.2e-08 EF-hand domain
EF-hand_8 PF13833 125 - 173 1.1e-07 EF-hand domain pair
EF-hand_1 PF00036 148 - 175 7.1e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 147
AbsI CCTCGAGG 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 115
AccB1I GGYRCC 1 cut(s) 56
AccBSI CCGCTC 1 cut(s) 62
AccI GTMKAC 2 cut(s) 249, 357
AccII CGCG 4 cut(s) 100, 185, 389, 532
AciI CCGC 8 cut(s) 60, 71, 79, 100, 318, 387, 389, 462
AclWI GGATC 2 cut(s) 232, 317
AcsI RAATTY 1 cut(s) 137
AcyI GRCGYC 5 cut(s) 57, 144, 252, 345, 465
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 4 cut(s) 10, 94, 133, 280
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 6 cut(s) 118, 178, 226, 295, 397, 417
AluI AGCT 6 cut(s) 118, 178, 226, 295, 397, 417
Alw21I GWGCWC 4 cut(s) 180, 228, 297, 434
AlwI GGATC 2 cut(s) 232, 317
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 137
AspLEI GCGC 4 cut(s) 53, 59, 106, 185
AspS9I GGNCC 2 cut(s) 202, 231
AsuC2I CCSGG 2 cut(s) 25, 209
AsuHPI GGTGA 2 cut(s) 75, 85
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 1 cut(s) 231
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 3 cut(s) 180, 228, 297
BbsI GAAGAC 1 cut(s) 121
Bbv12I GWGCWC 4 cut(s) 180, 228, 297, 434
BbvCI CCTCAGC 1 cut(s) 410
BbvI GCAGC 1 cut(s) 207
BccI CCATC 1 cut(s) 517
BceAI ACGGC 7 cut(s) 166, 172, 274, 280, 385, 391, 493
BciT130I CCWGG 1 cut(s) 200
BclI TGATCA 2 cut(s) 456, 489
BcnI CCSGG 2 cut(s) 25, 209
BfoI RGCGCY 2 cut(s) 54, 60
BfuAI ACCTGC 1 cut(s) 115
BglII AGATCT 1 cut(s) 162
BisI GCNGC 3 cut(s) 60, 196, 463
BlpI GCTNAGC 1 cut(s) 63
BlsI GCNGC 3 cut(s) 61, 197, 464
Bme1390I CCNGG 3 cut(s) 25, 200, 209
Bme18I GGWCC 1 cut(s) 231
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 2 cut(s) 202, 231
BmiI GGNNCC 1 cut(s) 58
BmrFI CCNGG 3 cut(s) 25, 200, 209
BmsI GCATC 2 cut(s) 66, 460
BpiI GAAGAC 1 cut(s) 121
BpmI CTGGAG 2 cut(s) 31, 140
Bpu10I CCTNAGC 1 cut(s) 410
Bpu1102I GCTNAGC 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 323
BpuMI CCSGG 2 cut(s) 25, 209
BsaHI GRCGYC 5 cut(s) 57, 144, 252, 345, 465
BsaJI CCNNGG 3 cut(s) 199, 321, 387
BsaWI WCCGGW 1 cut(s) 228
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse118I RCCGGY 2 cut(s) 53, 67
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 3 cut(s) 199, 321, 387
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMII CTCAG 2 cut(s) 77, 401
BseRI GAGGAG 3 cut(s) 407, 427, 515
BseXI GCAGC 1 cut(s) 207
Bsh1236I CGCG 4 cut(s) 100, 185, 389, 532
Bsh1285I CGRYCG 1 cut(s) 438
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 56
BsiEI CGRYCG 1 cut(s) 438
BsiHKAI GWGCWC 4 cut(s) 180, 228, 297, 434
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 6 cut(s) 25, 54, 68, 209, 229, 448
BslI CCNNNNNNNGG 1 cut(s) 214
BsnI GGCC 1 cut(s) 204
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 4 cut(s) 180, 228, 297, 434
Bsp143I GATC 9 cut(s) 108, 162, 186, 237, 309, 381, 435, 456, 489
Bsp1720I GCTNAGC 1 cut(s) 63
BspACI CCGC 8 cut(s) 60, 71, 79, 100, 318, 387, 389, 462
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 2 cut(s) 76, 402
BspFNI CGCG 4 cut(s) 100, 185, 389, 532
BspLI GGNNCC 1 cut(s) 58
BspMI ACCTGC 1 cut(s) 115
BspPI GGATC 2 cut(s) 232, 317
BspT107I GGYRCC 1 cut(s) 56
BsrBI CCGCTC 1 cut(s) 62
BsrFI RCCGGY 2 cut(s) 53, 67
BssAI RCCGGY 2 cut(s) 53, 67
BssECI CCNNGG 3 cut(s) 199, 321, 387
BssMI GATC 9 cut(s) 108, 162, 186, 237, 309, 381, 435, 456, 489
BssNI GRCGYC 5 cut(s) 57, 144, 252, 345, 465
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 2 cut(s) 87, 485
Bst6I CTCTTC 1 cut(s) 23
BstACI GRCGYC 5 cut(s) 57, 144, 252, 345, 465
BstC8I GCNNGC 3 cut(s) 55, 69, 102
BstDEI CTNAG 2 cut(s) 63, 410
BstDSI CCRYGG 1 cut(s) 387
BstEII GGTNACC 1 cut(s) 81
BstFNI CGCG 4 cut(s) 100, 185, 389, 532
BstH2I RGCGCY 2 cut(s) 54, 60
BstHHI GCGC 4 cut(s) 53, 59, 106, 185
BstKTI GATC 9 cut(s) 111, 165, 189, 240, 312, 384, 438, 459, 492
BstMBI GATC 9 cut(s) 108, 162, 186, 237, 309, 381, 435, 456, 489
BstMCI CGRYCG 1 cut(s) 438
BstMWI GCNNNNNNNGC 6 cut(s) 59, 68, 110, 201, 429, 438
BstNI CCWGG 1 cut(s) 200
BstPI GGTNACC 1 cut(s) 81
BstSCI CCNGG 3 cut(s) 23, 198, 207
BstUI CGCG 4 cut(s) 100, 185, 389, 532
BstV1I GCAGC 1 cut(s) 207
BstV2I GAAGAC 1 cut(s) 121
BstX2I RGATCY 1 cut(s) 162
BstYI RGATCY 1 cut(s) 162
BsuRI GGCC 1 cut(s) 204
BtgI CCRYGG 1 cut(s) 387
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 3 cut(s) 55, 69, 102
CfoI GCGC 4 cut(s) 53, 59, 106, 185
Cfr10I RCCGGY 2 cut(s) 53, 67
Cfr13I GGNCC 2 cut(s) 202, 231
Cfr42I CCGCGG 1 cut(s) 390
CseI GACGC 3 cut(s) 260, 353, 454
CviAII CATG 2 cut(s) 190, 235
DdeI CTNAG 2 cut(s) 63, 410
DinI GGCGCC 1 cut(s) 58
DpnI GATC 9 cut(s) 110, 164, 188, 239, 311, 383, 437, 458, 491
DpnII GATC 9 cut(s) 108, 162, 186, 237, 309, 381, 435, 456, 489
DraI TTTAAA 1 cut(s) 508
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 307
Ecl136II GAGCTC 3 cut(s) 178, 226, 295
Eco24I GRGCYC 3 cut(s) 180, 228, 297
Eco47I GGWCC 1 cut(s) 231
Eco53kI GAGCTC 3 cut(s) 178, 226, 295
Eco88I CYCGRG 1 cut(s) 242
Eco91I GGTNACC 1 cut(s) 81
EcoICRI GAGCTC 3 cut(s) 178, 226, 295
EcoO65I GGTNACC 1 cut(s) 81
EcoRII CCWGG 1 cut(s) 198
EcoT38I GRGCYC 3 cut(s) 180, 228, 297
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
FaeI CATG 2 cut(s) 193, 238
FaiI YATR 4 cut(s) 191, 236, 488, 537
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FatI CATG 2 cut(s) 189, 234
FauI CCCGC 1 cut(s) 93
FbaI TGATCA 2 cut(s) 456, 489
FblI GTMKAC 2 cut(s) 249, 357
Fnu4HI GCNGC 3 cut(s) 60, 196, 463
FriOI GRGCYC 3 cut(s) 180, 228, 297
Fsp4HI GCNGC 3 cut(s) 60, 196, 463
GlaI GCGC 4 cut(s) 52, 58, 105, 184
GluI GCNGC 3 cut(s) 60, 196, 463
GsuI CTGGAG 2 cut(s) 31, 140
HaeII RGCGCY 2 cut(s) 54, 60
HaeIII GGCC 1 cut(s) 204
HapII CCGG 6 cut(s) 25, 54, 68, 209, 229, 448
HgaI GACGC 3 cut(s) 260, 353, 454
HhaI GCGC 4 cut(s) 53, 59, 106, 185
Hin1I GRCGYC 5 cut(s) 57, 144, 252, 345, 465
Hin1II CATG 2 cut(s) 193, 238
Hin6I GCGC 4 cut(s) 51, 57, 104, 183
HinP1I GCGC 4 cut(s) 51, 57, 104, 183
HincII GTYRAC 2 cut(s) 148, 250
HindII GTYRAC 2 cut(s) 148, 250
HinfI GANTC 2 cut(s) 314, 334
HpaII CCGG 6 cut(s) 25, 54, 68, 209, 229, 448
HphI GGTGA 2 cut(s) 75, 85
Hpy166II GTNNAC 3 cut(s) 148, 250, 358
Hpy188I TCNGA 1 cut(s) 175
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 3 cut(s) 148, 250, 358
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 2 cut(s) 87, 485
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 1 cut(s) 331
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 68, 110, 201, 429, 438
HpyF3I CTNAG 2 cut(s) 63, 410
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 5 cut(s) 57, 144, 252, 345, 465
Hsp92II CATG 2 cut(s) 193, 238
HspAI GCGC 4 cut(s) 51, 57, 104, 183
KasI GGCGCC 1 cut(s) 56
KroI GCCGGC 2 cut(s) 53, 67
KroNI GCCGGC 2 cut(s) 55, 69
Ksp22I TGATCA 2 cut(s) 456, 489
KspI CCGCGG 1 cut(s) 390
Kzo9I GATC 9 cut(s) 108, 162, 186, 237, 309, 381, 435, 456, 489
LmnI GCTCC 6 cut(s) 121, 175, 231, 292, 394, 414
Lsp1109I GCAGC 1 cut(s) 207
LweI GCATC 2 cut(s) 66, 460
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 1 cut(s) 81
MalI GATC 9 cut(s) 110, 164, 188, 239, 311, 383, 437, 458, 491
MbiI CCGCTC 1 cut(s) 62
MboI GATC 9 cut(s) 108, 162, 186, 237, 309, 381, 435, 456, 489
MboII GAAGA 4 cut(s) 10, 40, 43, 121
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 4 cut(s) 180, 228, 297, 434
MluCI AATT 3 cut(s) 137, 493, 568
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 153
MreI CGCCGGCG 1 cut(s) 53
MroNI GCCGGC 2 cut(s) 53, 67
MseI TTAA 1 cut(s) 507
MspA1I CMGCKG 2 cut(s) 389, 462
MspI CCGG 6 cut(s) 25, 54, 68, 209, 229, 448
MspR9I CCNGG 3 cut(s) 25, 200, 209
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 4 cut(s) 100, 185, 389, 532
MwoI GCNNNNNNNGC 6 cut(s) 59, 68, 110, 201, 429, 438
NaeI GCCGGC 2 cut(s) 55, 69
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 2 cut(s) 25, 209
NdeII GATC 9 cut(s) 108, 162, 186, 237, 309, 381, 435, 456, 489
NgoMIV GCCGGC 2 cut(s) 53, 67
NlaIII CATG 2 cut(s) 193, 238
NlaIV GGNNCC 1 cut(s) 58
NmeAIII GCCGAG 3 cut(s) 139, 159, 346
NmuCI GTSAC 1 cut(s) 81
PaeR7I CTCGAG 1 cut(s) 242
PcsI WCGNNNNNNNCGW 5 cut(s) 150, 258, 270, 318, 351
PdiI GCCGGC 2 cut(s) 55, 69
PfeI GAWTC 1 cut(s) 334
PfoI TCCNGGA 1 cut(s) 23
PkrI GCNGC 3 cut(s) 61, 197, 464
Ple19I CGATCG 1 cut(s) 438
PleI GAGTC 1 cut(s) 308
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 308
Psp124BI GAGCTC 3 cut(s) 180, 228, 297
Psp6I CCWGG 1 cut(s) 198
PspEI GGTNACC 1 cut(s) 81
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 58
PspPI GGNCC 2 cut(s) 202, 231
PspXI VCTCGAGB 1 cut(s) 242
PsuI RGATCY 1 cut(s) 162
PvuI CGATCG 1 cut(s) 438
SacI GAGCTC 3 cut(s) 180, 228, 297
SacII CCGCGG 1 cut(s) 390
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 3 cut(s) 60, 196, 463
Sau3AI GATC 9 cut(s) 108, 162, 186, 237, 309, 381, 435, 456, 489
Sau96I GGNCC 2 cut(s) 202, 231
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 3 cut(s) 25, 200, 209
SduI GDGCHC 4 cut(s) 180, 228, 297, 434
SfaNI GCATC 2 cut(s) 66, 460
SfoI GGCGCC 1 cut(s) 58
Sfr274I CTCGAG 1 cut(s) 242
Sfr303I CCGCGG 1 cut(s) 390
SgrAI CRCCGGYG 1 cut(s) 53
SgrBI CCGCGG 1 cut(s) 390
SinI GGWCC 1 cut(s) 231
SlaI CTCGAG 1 cut(s) 242
SmlI CTYRAG 2 cut(s) 242, 338
SmoI CTYRAG 2 cut(s) 242, 338
Sse9I AATT 3 cut(s) 137, 493, 568
SsiI CCGC 8 cut(s) 60, 71, 79, 100, 318, 387, 389, 462
SspDI GGCGCC 1 cut(s) 56
SstI GAGCTC 3 cut(s) 180, 228, 297
StyD4I CCNGG 3 cut(s) 23, 198, 207
TaaI ACNGT 2 cut(s) 87, 485
TaiI ACGT 1 cut(s) 147
TasI AATT 3 cut(s) 137, 493, 568
TauI GCSGC 2 cut(s) 62, 465
TfiI GAWTC 1 cut(s) 334
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TseFI GTSAC 1 cut(s) 81
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 1 cut(s) 81
TspDTI ATGAA 1 cut(s) 259
TspGWI ACGGA 2 cut(s) 230, 343
VpaK11BI GGWCC 1 cut(s) 231
XapI RAATTY 1 cut(s) 137
XhoI CTCGAG 1 cut(s) 242
XmiI GTMKAC 2 cut(s) 249, 357
ZraI GACGTC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.