Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0003291

Physical Location & Seq

Genomic Coordinates Forward (+)

51650447 .. 51654626

Gene Structure 4180 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0344500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2574 bp

ATGTTGGGTGAGGAAGATTCTGAGTGTGGGCTTGCCGCCGGAAATAGTACCCGGAAAAGTTATCTCCGGTCCATGTCATGGAGTGACCGTTCACCTTCCAAACCTAATCCTTATCCAAAGCCTCAGCTCAATAGTAAAGTAAGGTCTTGCTTGCCTCCACTTCAGCCTCTTTCAATAGCCAAGAGTAATGTTAAGGAGTGGCCAAAGGCGGGTTCTGATGATCTTGGTGTTTGGCCTCAGCCCCAAACCCCTAGAGGGTCGGCTAAACCCTTTCCGGGTTCGAATCCCCCCCAACTGCCTGTGCCTGGGAGAGAGTTTGAGTTTAAGAAAGATAAGCTTGCTTTTTTTGATAAAGAGTGCTCCAGAATTGCGGAGCATATATACTTGGGGAGTGATGCTGTGGCTAAGAACCGTGAGGTTTTGAGGAAGAACGGCATCACCCATGTGCTCAACTGTGTTGGGTTTGTGTCTCCTGAGTATTTCAAGAATGATCTTGTGTACAAGACGCTCTGGTTGAAAGATAGCCCCTCCGAGGACATTACGAGCATTCTGTATGATGTGTTTGATTATTTTGAAGATGTCAGGAAGCAAAGTGGGAGAGTTCTTGTGCATTGTTGTCAGGGAGTGTCGCGGTCGACCGCTCTCGTCATTGCATACCTTATGTGGAGGGAGGGCCAGAGCTTTGAAGATGCATTTCAGTATGTCAAGGCAGCAAGAGGGGTGACCAACCCGAATATGGGTTTTGCTTGTCAACTTCTGCAGTGCCAGAAGCGGGTGCATGCTGTGCCTGCAAGCCCGAGTTCTGTGTTAAGGATGTACAGGATGGCCCCCCATTCATCCTATGATCCTCTTCATCTGGTGCCAAAGATGTTAAACCATCCAGGTCCACAAGGACTTGACTCTCGTGGTGCGTTTGTTGTGCATGTTCCATCTGCTATATATGTCTGGATTGGAAAGGATTGCAACACAATGATGTCAGACAAAGCAAGAGCAGCTGCCCTTCAGGTCATCCAGTATGAGAGGGCAAAGGGTCCAACTGTGGACATCAATGAAGGCAAGGAGCCACCAGAATTTTGGGACGCTCTTTCCAGTGGTCTTTCAGCAGAGGATTGCGGCAAGGGAGATGTCCAAAACCAAAAGGAAGAAACTGTTTCTTCTGGAGGCGATAAGGTTGCTGCAGTGACTTGTATTCCGGTGGGTGAAAGAAAGGTTGGTGAATATGATCTGGATTTCGAAATTTTCAGTAAGGCACTTGCAGGTGGGGTTGTTCCACCATTTTCAGTATCAAATACTCAATCGGAAACTTGCCTTCCAGCCAGAGAAAATGGATGGGGCAGATTGCGGCAAAAGTTTGCTAGTGGAATTATGAAGGATTTGGTCACATCTTCTGAGCTGAACTGTAAAACTACCCCATCCAGTGATGGATTGGATATGGTTGTGGAAGTTAACAAAGAAGAAGAAGACCCTGTTTCCTTGACTGAGCCTTTATCACCATTGTCAGCGCCAAGCCACTTTTGTGGTTCACCAGATTCCTTTGAGTGTTATCCAATTAGTAGCCCAAGTAGGATAAGAGATACATGTCGGGAAGTAGGACACTCTGTTGCTCTTACCAATCCATTATCGTTTCCGACAACTGACTGTGGGTCACCCGATTCTTGTTCTTGGTTTCCTGATAACAATCTTAAGTTTAGCTCAAAATCCCCAACACTCTCCCCTTCAACCTCTGAATACTCTAGTTCATCATTTACCTTTTCACCCTCATCTTCTAATTGGTCGGACTTGTCATACTTGTCTTCTCAACAGCCTTCACCTTCAGGCTTGGATTCTGCAGATCCATTATGTATTAAGCATAGTTCCATAGCCGATAGCTCACAGTTACCTTTCAAAGAATCTCTTTCTTCACCTAGAGAGGCATTTTCAGCTCATCGTACTTTGGAAGTGACAAATACATGTTCGACATGTAAAGGGATTTCCCCTTCTATTGCAGAGCGTAGAGGGAGTAAACCTCCACCTCTAATGTTGCTACCCTCAGTTGAAGAATCACCTAAAGTTTCGAGGAATTTGGTACGCTCATGGTCGTTTTCCTTACCTGACTTGGATGATGATCTGATGGACACTGATTGCAACCAAGCCGAAAATGTGAGCAACAGAGAAGAGCTAATGTTGGATGTTGACATTATTAACCCTGAAAATGAATTACAATCTGAAGCGACCAGGGAAGCTGCAGAGGTGATTAACCCTGTTTTGTACCAGTGGCCTTCTTTGAACAAAGTGGTGATGCATCGGTCTGCCAAACTTCATTCTGGATCAGCATATCTTTTGCTAGCTCCAGATGCGACTGTGGGTGCACATAAACCTGGTGTCTTATTTGTCTGGTTGGGATGTGATATTTTGCATGAGAAAGGACAGAATGCAACATTTGAGGATAGCCATCTTCACTGGGAGACCATAGGACGCTGTTTCCTTGAGCATATGGGTTTGCCTATGAGTGCCCCTGTACAGATAGTAAGAGAAGGTGAGGAGCCAGAGCAGTTCCTGATCCATCTCAGCTGTAAATCAATACAGAACACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016049 GO:0021700 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0040007 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046872 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050896 GO:0060560 GO:0071695 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

857

Amino Acids

94.25

Weight (kDa)

5.47

Isoelectric Point (pI)

55.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DSPc	PF00782	127 - 257	3.6e-28	Dual specificity phosphatase, catalytic domain
Gelsolin	PF00626	299 - 359	5.5e-06	Gelsolin repeat
MPK1_gelsolin_C	PF25466	751 - 849	2.6e-27	MPK1 gelsolin domain C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003291)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24620
fragaria_vesca	FvH4_3g17660 FvH4_3g19960 FvH4_3g29250 FvH4_4g33650 FvH4_5g39020
malus_domestica	MD12G1080300.v1.1 MD13G1020800.v1.1 MD15G1357800.v1.1 MD16G1019200.v1.1
prunus_persica	Prupe.1G505300_v2.0.a1 Prupe.8G060000_v2.0.a1
pyrus_communis	pycom16g01720
rosa_chinensis	RchiOBHm_Chr4g0443111 RchiOBHm_Chr7g0240981
rosa_laevigata	RLG00000000607 RLG00000022992
rosa_multiflora	Rmu_co8148428.1_g000001 Rmu_co8155060.1_g000001 Rmu_sc0000677.1_g000036 Rmu_sc0003187.1_g000018 Rmu_sc0004091.1_g000001 Rmu_sc0007968.1_g000014 Rmu_sc0008069.1_g000001 Rmu_sc0042435.1_g000001
rosa_roxburghii	Rroxscaffold_3G00219340 Rroxscaffold_5G00336800
rosa_rugosa	Rorug04G0344500 Rorug07G0341200
rosa_samantha	Rh4BG410400 Rh4DG405400 Rh5DG466500 Rh7AG496000
rosa_wichuraiana	Rw4G034380 Rw5G040770 Rw7G041910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1247
Acc36I	ACCTGC	1 cut(s)	1247
AccB1I	GGYRCC	1 cut(s)	859
AccB7I	CCANNNNNTGG	1 cut(s)	78
AccBSI	CCGCTC	1 cut(s)	641
AccI	GTMKAC	1 cut(s)	635
AccII	CGCG	1 cut(s)	631
AciI	CCGC	8 cut(s)	36, 209, 371, 631, 639, 772, 1113, 1342
AclWI	GGATC	4 cut(s)	839, 1826, 2314, 2533
AcoI	YGGCCR	1 cut(s)	200
AcsI	RAATTY	3 cut(s)	1070, 1236, 2059
AcuI	CTGAAG	4 cut(s)	146, 986, 1797, 2226
AfaI	GTAC	7 cut(s)	49, 500, 818, 1930, 2067, 2249, 2499
AfiI	CCNNNNNNNGG	9 cut(s)	78, 209, 255, 275, 305, 532, 736, 737, 772
AflII	CTTAAG	1 cut(s)	1682
AflIII	ACRYGT	3 cut(s)	1577, 1949, 1958
AgsI	TTSAA	9 cut(s)	174, 484, 517, 575, 686, 1719, 1885, 2036, 2266
AhdI	GACNNNNNGTC	1 cut(s)	1642
AjnI	CCWGG	4 cut(s)	304, 880, 2213, 2356
AjuI	GAANNNNNNNTTGG	4 cut(s)	196, 228, 1194, 1226
AleI	CACNNNNGTG	2 cut(s)	443, 1515
AloI	GAACNNNNNNTCC	2 cut(s)	73, 105
Alw21I	GWGCWC	3 cut(s)	362, 450, 2350
Alw26I	GTCTC	2 cut(s)	474, 2438
Alw44I	GTGCAC	1 cut(s)	2346
AlwI	GGATC	4 cut(s)	839, 1826, 2314, 2533
AlwNI	CAGNNNCTG	1 cut(s)	2536
Ama87I	CYCGRG	1 cut(s)	796
AoxI	GGCC	5 cut(s)	200, 233, 673, 825, 2255
ApaLI	GTGCAC	1 cut(s)	2346
ApeKI	GCWGC	5 cut(s)	710, 992, 995, 1175, 2222
ApoI	RAATTY	3 cut(s)	1070, 1236, 2059
AspLEI	GCGC	1 cut(s)	1504
AspS9I	GGNCC	5 cut(s)	69, 673, 826, 884, 1031
AsuC2I	CCSGG	2 cut(s)	52, 276
AsuII	TTCGAA	2 cut(s)	281, 1233
AsuNHI	GCTAGC	1 cut(s)	2323
AvaI	CYCGRG	1 cut(s)	796
AvaII	GGWCC	3 cut(s)	69, 884, 1031
BaeGI	GKGCMC	2 cut(s)	2350, 2494
BalI	TGGCCA	1 cut(s)	202
BanI	GGYRCC	1 cut(s)	859
BauI	CACGAG	1 cut(s)	903
BbsI	GAAGAC	2 cut(s)	1467, 1785
Bbv12I	GWGCWC	3 cut(s)	362, 450, 2350
BbvCI	CCTCAGC	2 cut(s)	123, 237
BbvI	GCAGC	5 cut(s)	722, 982, 1004, 1162, 2209
BccI	CCATC	9 cut(s)	817, 885, 937, 1323, 1415, 1420, 2104, 2439, 2550
BceAI	ACGGC	1 cut(s)	448
BcgI	CGANNNNNNTGC	2 cut(s)	1154, 1188
BciT130I	CCWGG	4 cut(s)	306, 882, 2215, 2358
BcnI	CCSGG	2 cut(s)	52, 276
BcoDI	GTCTC	2 cut(s)	474, 2438
BfaI	CTAG	5 cut(s)	252, 1356, 1734, 1905, 2324
BfmI	CTRYAG	4 cut(s)	758, 1176, 1827, 2223
BfoI	RGCGCY	1 cut(s)	1505
BfrI	CTTAAG	1 cut(s)	1682
BfuAI	ACCTGC	1 cut(s)	1247
BisI	GCNGC	8 cut(s)	36, 711, 993, 996, 1114, 1176, 1343, 2223
BlsI	GCNGC	8 cut(s)	37, 712, 994, 997, 1115, 1177, 1344, 2224
Bme1390I	CCNGG	6 cut(s)	52, 276, 306, 882, 2215, 2358
Bme18I	GGWCC	3 cut(s)	69, 884, 1031
BmeRI	GACNNNNNGTC	1 cut(s)	1642
BmeT110I	CYCGRG	1 cut(s)	796
BmgT120I	GGNCC	5 cut(s)	69, 673, 826, 884, 1031
BmiI	GGNNCC	5 cut(s)	828, 861, 1032, 1062, 2523
BmrFI	CCNGG	6 cut(s)	52, 276, 306, 882, 2215, 2358
BmrI	ACTGGG	1 cut(s)	2449
BmsI	GCATC	6 cut(s)	385, 444, 679, 2268, 2290, 2323
BmtI	GCTAGC	1 cut(s)	2327
BmuI	ACTGGG	1 cut(s)	2449
BpiI	GAAGAC	2 cut(s)	1467, 1785
BplI	GAGNNNNNCTC	2 cut(s)	1990, 2022
BpmI	CTGGAG	3 cut(s)	346, 1179, 2313
Bpu10I	CCTNAGC	2 cut(s)	123, 237
Bpu14I	TTCGAA	2 cut(s)	281, 1233
BpuEI	CTTGAG	1 cut(s)	2486
BpuMI	CCSGG	2 cut(s)	52, 276
BsaBI	GATNNNNATC	3 cut(s)	1677, 2103, 2430
BsaI	GGTCTC	1 cut(s)	2438
BsaJI	CCNNGG	3 cut(s)	305, 531, 2214
BsaWI	WCCGGW	2 cut(s)	66, 1192
BsaXI	ACNNNNNCTCC	2 cut(s)	73, 103
Bsc4I	CCNNNNNNNGG	9 cut(s)	78, 209, 255, 275, 305, 532, 736, 737, 772
Bse1I	ACTGG	5 cut(s)	1012, 1089, 1416, 2251, 2444
Bse3DI	GCAATG	1 cut(s)	648
Bse8I	GATNNNNATC	3 cut(s)	1677, 2103, 2430
BseBI	CCWGG	4 cut(s)	306, 882, 2215, 2358
BseDI	CCNNGG	3 cut(s)	305, 531, 2214
BseJI	GATNNNNATC	3 cut(s)	1677, 2103, 2430
BseLI	CCNNNNNNNGG	9 cut(s)	78, 209, 255, 275, 305, 532, 736, 737, 772
BseMI	GCAATG	1 cut(s)	648
BseMII	CTCAG	8 cut(s)	12, 137, 251, 465, 1380, 1470, 2043, 2560
BseNI	ACTGG	5 cut(s)	1012, 1089, 1416, 2251, 2444
BseRI	GAGGAG	1 cut(s)	2534
BseSI	GKGCMC	2 cut(s)	2350, 2494
BseXI	GCAGC	5 cut(s)	722, 982, 1004, 1162, 2209
Bsh1236I	CGCG	1 cut(s)	631
Bsh1285I	CGRYCG	2 cut(s)	635, 639
BshFI	GGCC	5 cut(s)	202, 235, 675, 827, 2257
BshNI	GGYRCC	1 cut(s)	859
BsiEI	CGRYCG	2 cut(s)	635, 639
BsiHKAI	GWGCWC	3 cut(s)	362, 450, 2350
BsiHKCI	CYCGRG	1 cut(s)	796
BsiSI	CCGG	5 cut(s)	39, 52, 67, 275, 1193
BslFI	GGGAC	1 cut(s)	1091
BslI	CCNNNNNNNGG	9 cut(s)	78, 209, 255, 275, 305, 532, 736, 737, 772
BsmAI	GTCTC	2 cut(s)	474, 2438
BsmFI	GGGAC	1 cut(s)	1091
BsmI	GAATGC	2 cut(s)	546, 2416
BsnI	GGCC	5 cut(s)	202, 235, 675, 827, 2257
Bso31I	GGTCTC	1 cut(s)	2438
BsoBI	CYCGRG	1 cut(s)	796
Bsp119I	TTCGAA	2 cut(s)	281, 1233
Bsp1286I	GDGCHC	4 cut(s)	362, 450, 2350, 2494
Bsp1407I	TGTACA	3 cut(s)	498, 816, 2497
Bsp143I	GATC	8 cut(s)	220, 490, 844, 1222, 1831, 2104, 2306, 2538
BspACI	CCGC	8 cut(s)	36, 209, 371, 631, 639, 772, 1113, 1342
BspANI	GGCC	5 cut(s)	202, 235, 675, 827, 2257
BspCNI	CTCAG	8 cut(s)	13, 136, 250, 466, 1381, 1471, 2042, 2559
BspFNI	CGCG	1 cut(s)	631
BspLI	GGNNCC	5 cut(s)	828, 861, 1032, 1062, 2523
BspMAI	CTGCAG	4 cut(s)	762, 1180, 1831, 2227
BspMI	ACCTGC	1 cut(s)	1247
BspOI	GCTAGC	1 cut(s)	2327
BspPI	GGATC	4 cut(s)	839, 1826, 2314, 2533
BspQI	GCTCTTC	1 cut(s)	2148
BspT104I	TTCGAA	2 cut(s)	281, 1233
BspT107I	GGYRCC	1 cut(s)	859
BspTI	CTTAAG	1 cut(s)	1682
BspTNI	GGTCTC	1 cut(s)	2438
BsrBI	CCGCTC	1 cut(s)	641
BsrDI	GCAATG	1 cut(s)	648
BsrGI	TGTACA	3 cut(s)	498, 816, 2497
BsrI	ACTGG	5 cut(s)	1012, 1089, 1416, 2251, 2444
BssECI	CCNNGG	3 cut(s)	305, 531, 2214
BssMI	GATC	8 cut(s)	220, 490, 844, 1222, 1831, 2104, 2306, 2538
BssSI	CACGAG	1 cut(s)	903
Bst2BI	CACGAG	1 cut(s)	903
Bst2UI	CCWGG	4 cut(s)	306, 882, 2215, 2358
Bst4CI	ACNGT	9 cut(s)	89, 413, 455, 1039, 1150, 1400, 1640, 1875, 2341
Bst6I	CTCTTC	2 cut(s)	855, 2148
BstAFI	CTTAAG	1 cut(s)	1682
BstAPI	GCANNNNNTGC	1 cut(s)	784
BstAUI	TGTACA	3 cut(s)	498, 816, 2497
BstBI	TTCGAA	2 cut(s)	281, 1233
BstC8I	GCNNGC	7 cut(s)	33, 152, 339, 780, 789, 793, 2325
BstDEI	CTNAG	9 cut(s)	21, 123, 237, 405, 474, 1389, 1479, 2029, 2546
BstEII	GGTNACC	2 cut(s)	721, 1644
BstFNI	CGCG	1 cut(s)	631
BstH2I	RGCGCY	1 cut(s)	1505
BstHHI	GCGC	1 cut(s)	1504
BstKTI	GATC	8 cut(s)	223, 493, 847, 1225, 1834, 2107, 2309, 2541
BstMAI	GTCTC	2 cut(s)	474, 2438
BstMBI	GATC	8 cut(s)	220, 490, 844, 1222, 1831, 2104, 2306, 2538
BstMCI	CGRYCG	2 cut(s)	635, 639
BstMWI	GCNNNNNNNGC	5 cut(s)	784, 788, 992, 1919, 2333
BstNI	CCWGG	4 cut(s)	306, 882, 2215, 2358
BstNSI	RCATGY	5 cut(s)	782, 926, 1581, 1953, 1962
BstPI	GGTNACC	2 cut(s)	721, 1644
BstSCI	CCNGG	6 cut(s)	50, 274, 304, 880, 2213, 2356
BstSFI	CTRYAG	4 cut(s)	758, 1176, 1827, 2223
BstSLI	GKGCMC	2 cut(s)	2350, 2494
BstUI	CGCG	1 cut(s)	631
BstV1I	GCAGC	5 cut(s)	722, 982, 1004, 1162, 2209
BstV2I	GAAGAC	2 cut(s)	1467, 1785
BstX2I	RGATCY	1 cut(s)	1831
BstXI	CCANNNNNNTGG	2 cut(s)	1074, 1517
BstYI	RGATCY	1 cut(s)	1831
BsuRI	GGCC	5 cut(s)	202, 235, 675, 827, 2257
BtsI	GCAGTG	2 cut(s)	767, 1185
BtsIMutI	CAGTG	7 cut(s)	767, 1096, 1185, 1423, 2115, 2258, 2437
BveI	ACCTGC	1 cut(s)	1247
Cac8I	GCNNGC	7 cut(s)	33, 152, 339, 780, 789, 793, 2325
CaiI	CAGNNNCTG	1 cut(s)	2536
CfoI	GCGC	1 cut(s)	1504
Cfr13I	GGNCC	5 cut(s)	69, 673, 826, 884, 1031
CseI	GACGC	3 cut(s)	514, 1088, 2463
CsiI	ACCWGGT	1 cut(s)	2356
Csp6I	GTAC	7 cut(s)	48, 499, 817, 1929, 2066, 2248, 2498
CviQI	GTAC	7 cut(s)	48, 499, 817, 1929, 2066, 2248, 2498
DdeI	CTNAG	9 cut(s)	21, 123, 237, 405, 474, 1389, 1479, 2029, 2546
DpnI	GATC	8 cut(s)	222, 492, 846, 1224, 1833, 2106, 2308, 2540
DpnII	GATC	8 cut(s)	220, 490, 844, 1222, 1831, 2104, 2306, 2538
DriI	GACNNNNNGTC	1 cut(s)	1642
EaeI	YGGCCR	1 cut(s)	200
Eam1104I	CTCTTC	2 cut(s)	855, 2148
Eam1105I	GACNNNNNGTC	1 cut(s)	1642
EarI	CTCTTC	2 cut(s)	855, 2148
Eco31I	GGTCTC	1 cut(s)	2438
Eco47I	GGWCC	3 cut(s)	69, 884, 1031
Eco57I	CTGAAG	4 cut(s)	146, 986, 1797, 2226
Eco88I	CYCGRG	1 cut(s)	796
Eco91I	GGTNACC	2 cut(s)	721, 1644
EcoO65I	GGTNACC	2 cut(s)	721, 1644
EcoRII	CCWGG	4 cut(s)	304, 880, 2213, 2356
EcoT22I	ATGCAT	2 cut(s)	694, 2283
FalI	AAGNNNNNCTT	4 cut(s)	321, 353, 1878, 1910
FaqI	GGGAC	1 cut(s)	1091
FauI	CCCGC	2 cut(s)	202, 765
FauNDI	CATATG	1 cut(s)	2472
FblI	GTMKAC	1 cut(s)	635
Fnu4HI	GCNGC	8 cut(s)	36, 711, 993, 996, 1114, 1176, 1343, 2223
Fsp4HI	GCNGC	8 cut(s)	36, 711, 993, 996, 1114, 1176, 1343, 2223
FspBI	CTAG	5 cut(s)	252, 1356, 1734, 1905, 2324
GlaI	GCGC	1 cut(s)	1503
GluI	GCNGC	8 cut(s)	36, 711, 993, 996, 1114, 1176, 1343, 2223
GsuI	CTGGAG	3 cut(s)	346, 1179, 2313
HaeII	RGCGCY	1 cut(s)	1505
HaeIII	GGCC	5 cut(s)	202, 235, 675, 827, 2257
HapII	CCGG	5 cut(s)	39, 52, 67, 275, 1193
HgaI	GACGC	3 cut(s)	514, 1088, 2463
HhaI	GCGC	1 cut(s)	1504
Hin6I	GCGC	1 cut(s)	1502
HinP1I	GCGC	1 cut(s)	1502
HincII	GTYRAC	4 cut(s)	636, 752, 1447, 2173
HindII	GTYRAC	4 cut(s)	636, 752, 1447, 2173
HindIII	AAGCTT	1 cut(s)	335
HinfI	GANTC	8 cut(s)	17, 283, 899, 1529, 1652, 1823, 1889, 2039
HpaI	GTTAAC	1 cut(s)	1447
HpaII	CCGG	5 cut(s)	39, 52, 67, 275, 1193
HpyCH4III	ACNGT	9 cut(s)	89, 413, 455, 1039, 1150, 1400, 1640, 1875, 2341
HpyF10VI	GCNNNNNNNGC	5 cut(s)	784, 788, 992, 1919, 2333
HpyF3I	CTNAG	9 cut(s)	21, 123, 237, 405, 474, 1389, 1479, 2029, 2546
HspAI	GCGC	1 cut(s)	1502
KspAI	GTTAAC	1 cut(s)	1447
Kzo9I	GATC	8 cut(s)	220, 490, 844, 1222, 1831, 2104, 2306, 2538
LguI	GCTCTTC	1 cut(s)	2148
LmnI	GCTCC	5 cut(s)	365, 373, 1060, 2332, 2521
Lsp1109I	GCAGC	5 cut(s)	722, 982, 1004, 1162, 2209
LweI	GCATC	6 cut(s)	385, 444, 679, 2268, 2290, 2323
MabI	ACCWGGT	1 cut(s)	2356
MaeI	CTAG	5 cut(s)	252, 1356, 1734, 1905, 2324
MaeIII	GTNAC	7 cut(s)	83, 721, 1180, 1378, 1644, 1875, 1939
MalI	GATC	8 cut(s)	222, 492, 846, 1224, 1833, 2106, 2308, 2540
MbiI	CCGCTC	1 cut(s)	641
MboI	GATC	8 cut(s)	220, 490, 844, 1222, 1831, 2104, 2306, 2538
MflI	RGATCY	1 cut(s)	1831
MhlI	GDGCHC	4 cut(s)	362, 450, 2350, 2494
MlsI	TGGCCA	1 cut(s)	202
MluCI	AATT	8 cut(s)	366, 1070, 1236, 1362, 1548, 1768, 2059, 2195
MluNI	TGGCCA	1 cut(s)	202
MlyI	GAGTC	1 cut(s)	893
MmeI	TCCRAC	4 cut(s)	1058, 1652, 1755, 2145
Mox20I	TGGCCA	1 cut(s)	202
Mph1103I	ATGCAT	2 cut(s)	694, 2283
MscI	TGGCCA	1 cut(s)	202
MseI	TTAA	9 cut(s)	192, 324, 809, 872, 1446, 1683, 1845, 2181, 2235
MslI	CAYNNNNRTG	3 cut(s)	443, 971, 1515
Msp20I	TGGCCA	1 cut(s)	202
MspA1I	CMGCKG	2 cut(s)	995, 2550
MspCI	CTTAAG	1 cut(s)	1682
MspI	CCGG	5 cut(s)	39, 52, 67, 275, 1193
MspR9I	CCNGG	6 cut(s)	52, 276, 306, 882, 2215, 2358
Mva1269I	GAATGC	2 cut(s)	546, 2416
MvaI	CCWGG	4 cut(s)	306, 882, 2215, 2358
MvnI	CGCG	1 cut(s)	631
MwoI	GCNNNNNNNGC	5 cut(s)	784, 788, 992, 1919, 2333
NciI	CCSGG	2 cut(s)	52, 276
NdeI	CATATG	1 cut(s)	2472
NdeII	GATC	8 cut(s)	220, 490, 844, 1222, 1831, 2104, 2306, 2538
NheI	GCTAGC	1 cut(s)	2323
NlaIV	GGNNCC	5 cut(s)	828, 861, 1032, 1062, 2523
NmuCI	GTSAC	6 cut(s)	83, 721, 1180, 1378, 1644, 1939
NsiI	ATGCAT	2 cut(s)	694, 2283
NspI	RCATGY	5 cut(s)	782, 926, 1581, 1953, 1962
NspV	TTCGAA	2 cut(s)	281, 1233
OliI	CACNNNNGTG	2 cut(s)	443, 1515
PaeI	GCATGC	1 cut(s)	782
PaqCI	CACCTGC	1 cut(s)	1247
PciI	ACATGT	3 cut(s)	1577, 1949, 1958
PciSI	GCTCTTC	1 cut(s)	2148
PctI	GAATGC	2 cut(s)	546, 2416
PfeI	GAWTC	7 cut(s)	17, 283, 1529, 1652, 1823, 1889, 2039
PflMI	CCANNNNNTGG	1 cut(s)	78
PkrI	GCNGC	8 cut(s)	37, 712, 994, 997, 1115, 1177, 1344, 2224
PleI	GAGTC	1 cut(s)	893
PpsI	GAGTC	1 cut(s)	893
PscI	ACATGT	3 cut(s)	1577, 1949, 1958
Psp6I	CCWGG	4 cut(s)	304, 880, 2213, 2356
PspEI	GGTNACC	2 cut(s)	721, 1644
PspGI	CCWGG	4 cut(s)	304, 880, 2213, 2356
PspN4I	GGNNCC	5 cut(s)	828, 861, 1032, 1062, 2523
PspPI	GGNCC	5 cut(s)	69, 673, 826, 884, 1031
PstI	CTGCAG	4 cut(s)	762, 1180, 1831, 2227
PstNI	CAGNNNCTG	1 cut(s)	2536
PsuI	RGATCY	1 cut(s)	1831
PvuII	CAGCTG	2 cut(s)	995, 2550
RsaI	GTAC	7 cut(s)	49, 500, 818, 1930, 2067, 2249, 2499
RsaNI	GTAC	7 cut(s)	48, 499, 817, 1929, 2066, 2248, 2498
RseI	CAYNNNNRTG	3 cut(s)	443, 971, 1515
SalI	GTCGAC	1 cut(s)	634
SapI	GCTCTTC	1 cut(s)	2148
SaqAI	TTAA	9 cut(s)	192, 324, 809, 872, 1446, 1683, 1845, 2181, 2235
SatI	GCNGC	8 cut(s)	36, 711, 993, 996, 1114, 1176, 1343, 2223
Sau3AI	GATC	8 cut(s)	220, 490, 844, 1222, 1831, 2104, 2306, 2538
Sau96I	GGNCC	5 cut(s)	69, 673, 826, 884, 1031
SchI	GAGTC	1 cut(s)	893
ScrFI	CCNGG	6 cut(s)	52, 276, 306, 882, 2215, 2358
SduI	GDGCHC	4 cut(s)	362, 450, 2350, 2494
SexAI	ACCWGGT	1 cut(s)	2356
SfaNI	GCATC	6 cut(s)	385, 444, 679, 2268, 2290, 2323
SfcI	CTRYAG	4 cut(s)	758, 1176, 1827, 2223
SfuI	TTCGAA	2 cut(s)	281, 1233
SinI	GGWCC	3 cut(s)	69, 884, 1031
SmiMI	CAYNNNNRTG	3 cut(s)	443, 971, 1515
SmlI	CTYRAG	2 cut(s)	1682, 2465
SmoI	CTYRAG	2 cut(s)	1682, 2465
SphI	GCATGC	1 cut(s)	782
Sse9I	AATT	8 cut(s)	366, 1070, 1236, 1362, 1548, 1768, 2059, 2195
SsiI	CCGC	8 cut(s)	36, 209, 371, 631, 639, 772, 1113, 1342
SspMI	CTAG	5 cut(s)	252, 1356, 1734, 1905, 2324
StyD4I	CCNGG	6 cut(s)	50, 274, 304, 880, 2213, 2356
TaaI	ACNGT	9 cut(s)	89, 413, 455, 1039, 1150, 1400, 1640, 1875, 2341
TaqI	TCGA	5 cut(s)	281, 635, 1233, 1955, 2054
TaqII	GACCGA	1 cut(s)	2274
TasI	AATT	8 cut(s)	366, 1070, 1236, 1362, 1548, 1768, 2059, 2195
TatI	WGTACW	3 cut(s)	498, 816, 2497
TauI	GCSGC	3 cut(s)	38, 1116, 1345
TfiI	GAWTC	7 cut(s)	17, 283, 1529, 1652, 1823, 1889, 2039
Tru1I	TTAA	9 cut(s)	192, 324, 809, 872, 1446, 1683, 1845, 2181, 2235
Tru9I	TTAA	9 cut(s)	192, 324, 809, 872, 1446, 1683, 1845, 2181, 2235
TscAI	CASTG	7 cut(s)	767, 1096, 1185, 1423, 2122, 2258, 2444
TseFI	GTSAC	6 cut(s)	83, 721, 1180, 1378, 1644, 1939
TseI	GCWGC	5 cut(s)	710, 992, 995, 1175, 2222
Tsp45I	GTSAC	6 cut(s)	83, 721, 1180, 1378, 1644, 1939
TspDTI	ATGAA	7 cut(s)	825, 842, 1065, 1382, 1728, 2208, 2288
TspRI	CASTG	7 cut(s)	767, 1096, 1185, 1423, 2122, 2258, 2444
Van91I	CCANNNNNTGG	1 cut(s)	78
Vha464I	CTTAAG	1 cut(s)	1682
VneI	GTGCAC	1 cut(s)	2346
VpaK11BI	GGWCC	3 cut(s)	69, 884, 1031
XapI	RAATTY	3 cut(s)	1070, 1236, 2059
XceI	RCATGY	5 cut(s)	782, 926, 1581, 1953, 1962
XcmI	CCANNNNNNNNNTGG	3 cut(s)	733, 1071, 1423
XmiI	GTMKAC	1 cut(s)	635
XspI	CTAG	5 cut(s)	252, 1356, 1734, 1905, 2324
Zsp2I	ATGCAT	2 cut(s)	694, 2283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.