MD15G1357800.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003291
Physical Location & Seq
Reverse (-)
43138770 .. 43139243
474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1357800.v1.1.491

Sequence Viewer

Length: 474 bp
ATGGCTCAGCCCAGCTTTCTCTCAGCAGAAACTGAGACACTGAGCCAAGTCCTCGGCCTAGTAGAAACATTCAGAGCTTTTGATTCAGACAATGATGGGAAAATTACTGCTGCAGAGCTTGGTGGGATTTTAGGGTCACTTGGGTACAACCCTAGTGAAGAAGATGTGAGGGCAATGATGCAGCAAGGGGACACGAACAACGACGGTTTCCTAAGCATCGAGGAGTTTTTGGAGATGAACACGAAGAACATGGAATTCGGAGGGCTTGAAAATGTTCTTAAGAATGCTTTTGAAGCGTTGGAGGTCGATGGGGATGAGGTTGTGACTGCAGAAGAGCTTTATGAGGTTATGGGAGATGACTTGGGGTTTGAGCTGACACTTGAGGATTGCCAGGGGATTATTGCTTCAATTGATATAGATGGGGATGGGGGAGTTAGCTATGATGACTTCAAACTTATAGTCAACTCACTCTGA

Protein Analysis

158

Amino Acids

17.0

Weight (kDa)

4.05

Isoelectric Point (pI)

26.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 20 - 48 1.4e-06 EF-hand domain
EF-hand_5 PF13202 20 - 41 3.5e-06 EF hand
EF-hand_1 PF00036 21 - 45 8.8e-06 EF hand domain
EF-hand_7 PF13499 22 - 79 1.5e-13 EF-hand domain pair
EF-hand_8 PF13833 32 - 79 2.8e-08 EF-hand domain pair
EF-hand_7 PF13499 90 - 151 4.8e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 254
AfaI GTAC 1 cut(s) 146
AflII CTTAAG 1 cut(s) 278
AgsI TTSAA 4 cut(s) 269, 293, 408, 451
AjnI CCWGG 1 cut(s) 390
AluBI AGCT 6 cut(s) 15, 77, 118, 337, 373, 438
AluI AGCT 6 cut(s) 15, 77, 118, 337, 373, 438
Alw26I GTCTC 1 cut(s) 29
AlwNI CAGNNNCTG 1 cut(s) 32
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 2 cut(s) 110, 181
ApoI RAATTY 1 cut(s) 254
ArsI GACNNNNNNTTYG 3 cut(s) 350, 382, 444
Asp700I GAANNNNTTC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 97, 193
BccI CCATC 4 cut(s) 89, 302, 413, 419
BciT130I CCWGG 1 cut(s) 392
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 2 cut(s) 59, 153
BfmI CTRYAG 2 cut(s) 111, 327
BfrI CTTAAG 1 cut(s) 278
BisI GCNGC 2 cut(s) 111, 182
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 2 cut(s) 112, 183
Bme1390I CCNGG 1 cut(s) 392
BmrFI CCNGG 1 cut(s) 392
BmsI GCATC 2 cut(s) 168, 225
Bpu10I CCTNAGC 1 cut(s) 212
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 401
BsaJI CCNNGG 2 cut(s) 52, 391
Bse3DI GCAATG 1 cut(s) 180
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 2 cut(s) 52, 391
BseGI GGATG 2 cut(s) 319, 430
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 4 cut(s) 20, 24, 32, 36
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 2 cut(s) 97, 193
BseYI CCCAGC 1 cut(s) 11
BshFI GGCC 1 cut(s) 57
BslFI GGGAC 1 cut(s) 203
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 203
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 1 cut(s) 57
Bsp1720I GCTNAGC 1 cut(s) 6
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 4 cut(s) 19, 25, 33, 35
BspMAI CTGCAG 2 cut(s) 115, 331
BspQI GCTCTTC 1 cut(s) 327
BspTI CTTAAG 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 180
BssECI CCNNGG 2 cut(s) 52, 391
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 1 cut(s) 206
Bst6I CTCTTC 1 cut(s) 327
BstAFI CTTAAG 1 cut(s) 278
BstDEI CTNAG 5 cut(s) 6, 22, 33, 41, 212
BstF5I GGATG 2 cut(s) 319, 430
BstMAI GTCTC 1 cut(s) 29
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 392
BstSCI CCNGG 1 cut(s) 390
BstSFI CTRYAG 2 cut(s) 111, 327
BstV1I GCAGC 2 cut(s) 97, 193
BsuRI GGCC 1 cut(s) 57
BtsCI GGATG 2 cut(s) 319, 430
BtsIMutI CAGTG 1 cut(s) 38
CaiI CAGNNNCTG 1 cut(s) 32
Csp6I GTAC 1 cut(s) 145
CviAII CATG 1 cut(s) 250
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 5 cut(s) 6, 22, 33, 41, 212
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 390
FaeI CATG 1 cut(s) 253
FaiI YATR 6 cut(s) 251, 342, 350, 416, 441, 458
FaqI GGGAC 1 cut(s) 203
FatI CATG 1 cut(s) 249
Fnu4HI GCNGC 2 cut(s) 111, 182
FokI GGATG 2 cut(s) 326, 437
Fsp4HI GCNGC 2 cut(s) 111, 182
FspBI CTAG 2 cut(s) 59, 153
GluI GCNGC 2 cut(s) 111, 182
GsaI CCCAGC 1 cut(s) 15
HaeIII GGCC 1 cut(s) 57
Hin1II CATG 1 cut(s) 253
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HinfI GANTC 1 cut(s) 83
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 4 cut(s) 74, 88, 260, 473
Hpy8I GTNNAC 1 cut(s) 463
Hpy99I CGWCG 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4V TGCA 3 cut(s) 113, 181, 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 5 cut(s) 6, 22, 33, 41, 212
Hsp92II CATG 1 cut(s) 253
LguI GCTCTTC 1 cut(s) 327
LpnPI CCDG 3 cut(s) 25, 377, 404
Lsp1109I GCAGC 2 cut(s) 97, 193
LweI GCATC 2 cut(s) 168, 225
MaeI CTAG 2 cut(s) 59, 153
MaeIII GTNAC 2 cut(s) 135, 322
MboII GAAGA 4 cut(s) 170, 173, 256, 344
MfeI CAATTG 1 cut(s) 408
MluCI AATT 3 cut(s) 102, 254, 408
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 8 cut(s) 62, 162, 214, 254, 295, 310, 337, 376
MroXI GAANNNNTTC 1 cut(s) 273
MseI TTAA 1 cut(s) 279
MspCI CTTAAG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 392
MunI CAATTG 1 cut(s) 408
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 1 cut(s) 392
MwoI GCNNNNNNNGC 1 cut(s) 293
NlaIII CATG 1 cut(s) 253
NmeAIII GCCGAG 1 cut(s) 33
NmuCI GTSAC 2 cut(s) 135, 322
PciSI GCTCTTC 1 cut(s) 327
PctI GAATGC 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 273
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 2 cut(s) 112, 183
Psp6I CCWGG 1 cut(s) 390
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 1 cut(s) 390
PstI CTGCAG 2 cut(s) 115, 331
PstNI CAGNNNCTG 1 cut(s) 32
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 2 cut(s) 111, 182
ScrFI CCNGG 1 cut(s) 392
SetI ASST 9 cut(s) 17, 79, 120, 306, 321, 339, 348, 375, 440
SfaNI GCATC 2 cut(s) 168, 225
SfcI CTRYAG 2 cut(s) 111, 327
SmlI CTYRAG 2 cut(s) 278, 380
SmoI CTYRAG 2 cut(s) 278, 380
Sse9I AATT 3 cut(s) 102, 254, 408
SspMI CTAG 2 cut(s) 59, 153
StyD4I CCNGG 1 cut(s) 390
TaaI ACNGT 1 cut(s) 206
TaqI TCGA 2 cut(s) 219, 306
TasI AATT 3 cut(s) 102, 254, 408
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 2 cut(s) 135, 322
TseI GCWGC 2 cut(s) 110, 181
Tsp45I GTSAC 2 cut(s) 135, 322
TspDTI ATGAA 1 cut(s) 251
TspRI CASTG 1 cut(s) 45
Vha464I CTTAAG 1 cut(s) 278
XapI RAATTY 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 273
XspI CTAG 2 cut(s) 59, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.