Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003291

Physical Location & Seq

Genomic Coordinates Reverse (-)

9791647 .. 9791973

Gene Structure 327 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1080300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 327 bp

ATGTTCTCCAAGGTCGATTCTGACGGAGACGGCTTCATCAACTTCAAAGAGTTCGTTGAGCTCAACACCAAAGGGGTCGACTTTGTCGAGGCCCTCGAAAATCCCAAAGACGCTTTCTCTGTCTACGACATTGATAGGAACGTGTCTATTTCGACGGAGGAATTGTACAAAGTGCTCCGGAGCTTGGGAGACGAATGTTTGATCGACGAGTGCCGGAAGATGATCGGCGGCGTCGACAGTGATGGTGACGGTATGATCAGAACCCAGTTGACATTCATGAGGCAAAACCTAGTTGACAAAACGAGCTTGATTGTGCCAAAGATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016049 GO:0021700 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0040007 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046872 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050896 GO:0060560 GO:0071695 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

109

Amino Acids

12.18

Weight (kDa)

4.43

Isoelectric Point (pI)

28.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_1	PF00036	1 - 21	9.3e-07	EF hand domain
EF-hand_7	PF13499	36 - 87	2.3e-06	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003291)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24620
fragaria_vesca	FvH4_3g17660 FvH4_3g19960 FvH4_3g29250 FvH4_4g33650 FvH4_5g39020
malus_domestica	MD12G1080300.v1.1 MD13G1020800.v1.1 MD15G1357800.v1.1 MD16G1019200.v1.1
prunus_persica	Prupe.1G505300_v2.0.a1 Prupe.8G060000_v2.0.a1
pyrus_communis	pycom16g01720
rosa_chinensis	RchiOBHm_Chr4g0443111 RchiOBHm_Chr7g0240981
rosa_laevigata	RLG00000000607 RLG00000022992
rosa_multiflora	Rmu_co8148428.1_g000001 Rmu_co8155060.1_g000001 Rmu_sc0000677.1_g000036 Rmu_sc0003187.1_g000018 Rmu_sc0004091.1_g000001 Rmu_sc0007968.1_g000014 Rmu_sc0008069.1_g000001 Rmu_sc0042435.1_g000001
rosa_roxburghii	Rroxscaffold_3G00219340 Rroxscaffold_5G00336800
rosa_rugosa	Rorug04G0344500 Rorug07G0341200
rosa_samantha	Rh4BG410400 Rh4DG405400 Rh5DG466500 Rh7AG496000
rosa_wichuraiana	Rw4G034380 Rw5G040770 Rw7G041910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	3 cut(s)	78, 123, 234
AccIII	TCCGGA	1 cut(s)	177
AciI	CCGC	1 cut(s)	228
AcyI	GRCGYC	1 cut(s)	231
AfaI	GTAC	1 cut(s)	167
AfiI	CCNNNNNNNGG	1 cut(s)	184
AflIII	ACRYGT	1 cut(s)	141
AgsI	TTSAA	1 cut(s)	46
AjuI	GAANNNNNNNTTGG	2 cut(s)	98, 130
AluBI	AGCT	3 cut(s)	61, 183, 306
AluI	AGCT	3 cut(s)	61, 183, 306
Alw21I	GWGCWC	2 cut(s)	63, 177
Alw26I	GTCTC	2 cut(s)	21, 183
Aor13HI	TCCGGA	1 cut(s)	177
AoxI	GGCC	1 cut(s)	90
AspS9I	GGNCC	1 cut(s)	91
AsuHPI	GGTGA	1 cut(s)	257
BanII	GRGCYC	1 cut(s)	63
Bbv12I	GWGCWC	2 cut(s)	63, 177
BccI	CCATC	1 cut(s)	236
BceAI	ACGGC	1 cut(s)	46
BclI	TGATCA	1 cut(s)	255
BcoDI	GTCTC	2 cut(s)	21, 183
BfaI	CTAG	2 cut(s)	290, 325
BglII	AGATCT	1 cut(s)	321
BisI	GCNGC	1 cut(s)	229
BlsI	GCNGC	1 cut(s)	230
BmgT120I	GGNCC	1 cut(s)	91
BmrI	ACTGGG	1 cut(s)	259
BmuI	ACTGGG	1 cut(s)	259
BsaHI	GRCGYC	1 cut(s)	231
BsaJI	CCNNGG	1 cut(s)	9
BsaWI	WCCGGW	1 cut(s)	177
BsaXI	ACNNNNNCTCC	2 cut(s)	149, 179
Bsc4I	CCNNNNNNNGG	1 cut(s)	184
Bse1I	ACTGG	1 cut(s)	265
BseAI	TCCGGA	1 cut(s)	177
BseDI	CCNNGG	1 cut(s)	9
BseLI	CCNNNNNNNGG	1 cut(s)	184
BseNI	ACTGG	1 cut(s)	265
BshFI	GGCC	1 cut(s)	92
BsiHKAI	GWGCWC	2 cut(s)	63, 177
BsiSI	CCGG	2 cut(s)	178, 214
BslI	CCNNNNNNNGG	1 cut(s)	184
BsmAI	GTCTC	2 cut(s)	21, 183
BsmBI	CGTCTC	2 cut(s)	21, 183
BsnI	GGCC	1 cut(s)	92
Bsp1286I	GDGCHC	2 cut(s)	63, 177
Bsp13I	TCCGGA	1 cut(s)	177
Bsp1407I	TGTACA	1 cut(s)	165
Bsp143I	GATC	4 cut(s)	201, 222, 255, 321
BspACI	CCGC	1 cut(s)	228
BspANI	GGCC	1 cut(s)	92
BspEI	TCCGGA	1 cut(s)	177
BspHI	TCATGA	1 cut(s)	276
BsrGI	TGTACA	1 cut(s)	165
BsrI	ACTGG	1 cut(s)	265
BssECI	CCNNGG	1 cut(s)	9
BssMI	GATC	4 cut(s)	201, 222, 255, 321
BssNI	GRCGYC	1 cut(s)	231
BssT1I	CCWWGG	1 cut(s)	9
Bst4CI	ACNGT	2 cut(s)	239, 251
BstACI	GRCGYC	1 cut(s)	231
BstAUI	TGTACA	1 cut(s)	165
BstKTI	GATC	4 cut(s)	204, 225, 258, 324
BstMAI	GTCTC	2 cut(s)	21, 183
BstMBI	GATC	4 cut(s)	201, 222, 255, 321
BstX2I	RGATCY	1 cut(s)	321
BstYI	RGATCY	1 cut(s)	321
BsuRI	GGCC	1 cut(s)	92
BtsIMutI	CAGTG	1 cut(s)	244
CciI	TCATGA	1 cut(s)	276
Cfr13I	GGNCC	1 cut(s)	91
CseI	GACGC	2 cut(s)	119, 220
Csp6I	GTAC	1 cut(s)	166
CviAII	CATG	1 cut(s)	277
CviJI	RGCY	5 cut(s)	33, 61, 92, 183, 306
CviKI_1	RGCY	5 cut(s)	33, 61, 92, 183, 306
CviQI	GTAC	1 cut(s)	166
DpnI	GATC	4 cut(s)	203, 224, 257, 323
DpnII	GATC	4 cut(s)	201, 222, 255, 321
Ecl136II	GAGCTC	1 cut(s)	61
Eco130I	CCWWGG	1 cut(s)	9
Eco24I	GRGCYC	1 cut(s)	63
Eco53kI	GAGCTC	1 cut(s)	61
EcoICRI	GAGCTC	1 cut(s)	61
EcoO109I	RGGNCCY	1 cut(s)	91
EcoT14I	CCWWGG	1 cut(s)	9
EcoT38I	GRGCYC	1 cut(s)	63
ErhI	CCWWGG	1 cut(s)	9
Esp3I	CGTCTC	2 cut(s)	21, 183
FaeI	CATG	1 cut(s)	280
FaiI	YATR	2 cut(s)	254, 278
FatI	CATG	1 cut(s)	276
FbaI	TGATCA	1 cut(s)	255
FblI	GTMKAC	3 cut(s)	78, 123, 234
Fnu4HI	GCNGC	1 cut(s)	229
FriOI	GRGCYC	1 cut(s)	63
Fsp4HI	GCNGC	1 cut(s)	229
FspBI	CTAG	2 cut(s)	290, 325
GluI	GCNGC	1 cut(s)	229
HaeIII	GGCC	1 cut(s)	92
HapII	CCGG	2 cut(s)	178, 214
HgaI	GACGC	2 cut(s)	119, 220
Hin1I	GRCGYC	1 cut(s)	231
Hin1II	CATG	1 cut(s)	280
HincII	GTYRAC	4 cut(s)	79, 235, 270, 295
HindII	GTYRAC	4 cut(s)	79, 235, 270, 295
HinfI	GANTC	1 cut(s)	17
HpaII	CCGG	2 cut(s)	178, 214
HphI	GGTGA	1 cut(s)	257
Hpy166II	GTNNAC	5 cut(s)	79, 124, 235, 270, 295
Hpy188I	TCNGA	2 cut(s)	22, 260
Hpy188III	TCNNGA	2 cut(s)	178, 277
Hpy8I	GTNNAC	5 cut(s)	79, 124, 235, 270, 295
Hpy99I	CGWCG	3 cut(s)	157, 209, 236
HpyCH4III	ACNGT	2 cut(s)	239, 251
HpyCH4IV	ACGT	1 cut(s)	141
HpySE526I	ACGT	1 cut(s)	141
Hsp92I	GRCGYC	1 cut(s)	231
Hsp92II	CATG	1 cut(s)	280
Kpn2I	TCCGGA	1 cut(s)	177
Ksp22I	TGATCA	1 cut(s)	255
Kzo9I	GATC	4 cut(s)	201, 222, 255, 321
LmnI	GCTCC	2 cut(s)	180, 180
LpnPI	CCDG	3 cut(s)	191, 227, 278
MaeI	CTAG	2 cut(s)	290, 325
MaeII	ACGT	1 cut(s)	141
MaeIII	GTNAC	1 cut(s)	245
MalI	GATC	4 cut(s)	203, 224, 257, 323
MboI	GATC	4 cut(s)	201, 222, 255, 321
MboII	GAAGA	1 cut(s)	229
MflI	RGATCY	1 cut(s)	321
MhlI	GDGCHC	2 cut(s)	63, 177
MluCI	AATT	1 cut(s)	161
MnlI	CCTC	4 cut(s)	82, 104, 151, 273
MroI	TCCGGA	1 cut(s)	177
MspI	CCGG	2 cut(s)	178, 214
NdeII	GATC	4 cut(s)	201, 222, 255, 321
NlaIII	CATG	1 cut(s)	280
NmuCI	GTSAC	1 cut(s)	245
PagI	TCATGA	1 cut(s)	276
PcsI	WCGNNNNNNNCGW	3 cut(s)	84, 93, 231
PfeI	GAWTC	1 cut(s)	17
PflFI	GACNNNGTC	1 cut(s)	83
PkrI	GCNGC	1 cut(s)	230
Psp124BI	GAGCTC	1 cut(s)	63
PspPI	GGNCC	1 cut(s)	91
PsuI	RGATCY	1 cut(s)	321
PsyI	GACNNNGTC	1 cut(s)	83
RsaI	GTAC	1 cut(s)	167
RsaNI	GTAC	1 cut(s)	166
SacI	GAGCTC	1 cut(s)	63
SalI	GTCGAC	2 cut(s)	77, 233
SatI	GCNGC	1 cut(s)	229
Sau3AI	GATC	4 cut(s)	201, 222, 255, 321
Sau96I	GGNCC	1 cut(s)	91
SduI	GDGCHC	2 cut(s)	63, 177
SetI	ASST	6 cut(s)	15, 63, 144, 185, 291, 308
Sse9I	AATT	1 cut(s)	161
SsiI	CCGC	1 cut(s)	228
SspMI	CTAG	2 cut(s)	290, 325
SstI	GAGCTC	1 cut(s)	63
StyI	CCWWGG	1 cut(s)	9
TaaI	ACNGT	2 cut(s)	239, 251
TaiI	ACGT	1 cut(s)	144
TaqI	TCGA	7 cut(s)	15, 78, 87, 96, 152, 204, 234
TasI	AATT	1 cut(s)	161
TatI	WGTACW	1 cut(s)	165
TauI	GCSGC	1 cut(s)	231
TfiI	GAWTC	1 cut(s)	17
TscAI	CASTG	1 cut(s)	244
TseFI	GTSAC	1 cut(s)	245
Tsp45I	GTSAC	1 cut(s)	245
TspDTI	ATGAA	2 cut(s)	25, 265
TspGWI	ACGGA	2 cut(s)	39, 170
TspRI	CASTG	1 cut(s)	244
Tth111I	GACNNNGTC	1 cut(s)	83
XmiI	GTMKAC	3 cut(s)	78, 123, 234
XspI	CTAG	2 cut(s)	290, 325

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.