FvH4_6g13020

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001859
Physical Location & Seq
Reverse (-)
7895936 .. 7916023
20088 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13020.t2

Sequence Viewer

Length: 963 bp
ATGGCGAACGACATTCGCTTCTTTGAGTTGAACACCGGAGCCAAGATGCCGTCTTTGGGCTTAGGGACCTGGCAGTCCAGCCCCGGCTTGGTCGGTGAAGCCGTCGCCACCGCCATCAAGGCAGGATATAGGCATATTGATTGTGCTCAAGTCTATGGCAATGAGAAAGAGATTGGTTTGGTTTTGAGGAAGCTGTTTGAAGATGGTGTGGTGAAGCGCGAGGAATTGTGGATTACCTCCAAGCTCTGGTGTACGGATCATGCACCGGAAGATGTACTAGAGGCATTGGATAGGACTTTGAGAGACCTGCAGCTTGAATATATTGACTTGTTCTTGATCCACTGGCCAGTGCGGATGAAGAAGGGTTCAGTAGGTTTTAAGCCTGAAAACCTTGTTGAACCAGATATCCCAGGTACTTGGAGAGCAATGGAAACTCTCTATGATTCGTGCAAGGCTCGAGCTATTGGTGTGAGCAATTTCTCTACAAAGAAGCTGTCAGATTTGTTGGATGTGGCACGTGTTCCTCCTGCTGTTGACCAAGTAGAGTGTCATCTTTCATGGCAGCAAGGCAAGCTACGATCCTTCTGCAAATCCAAGGGGGTTCACCTTTCTTTGTTCTTCTTTCAGGGATATTCACCACTGGGTTCTCCTGGCACGACATGGATCAAAAGCGATGTCCTTAAGAATCCGATTCTTCTCAGCGTCGCAGAGAAGCTAGGAAAAACTCCTGCTCAAGTTGCTCTTCGATGGGGACTGCAAATGGGTCACAGTGTTCTTCCAAAGAGTACTAACGAAGCAAGGATCAAGGAGAATCTTGATGTCTTCTGCTGGTCTATCCCAGAAGAATTGTTTGCCAAGTTTTCTGAAATTGAGCAGGCAAGGCTGCTGAGAGGGACCTCATTTGTTCATGACACCTTTGGACCCTATAGAAGTCTTGAAGAGCTTTGGGATGGTGAACTCTAG

Protein Analysis

321

Amino Acids

36.03

Weight (kDa)

6.2

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 19 - 290 1.2e-47 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 73
Acc36I ACCTGC 1 cut(s) 315
AccB7I CCANNNNNTGG 1 cut(s) 246
AccII CGCG 1 cut(s) 219
AciI CCGC 2 cut(s) 111, 352
AclWI GGATC 5 cut(s) 264, 331, 573, 671, 809
AcoI YGGCCR 1 cut(s) 344
AcvI CACGTG 1 cut(s) 518
AfaI GTAC 4 cut(s) 253, 276, 415, 787
AfiI CCNNNNNNNGG 3 cut(s) 56, 88, 246
AflII CTTAAG 1 cut(s) 680
AflIII ACRYGT 1 cut(s) 517
AgsI TTSAA 5 cut(s) 31, 200, 317, 398, 938
AjnI CCWGG 3 cut(s) 68, 409, 649
AloI GAACNNNNNNTCC 2 cut(s) 390, 422
AluBI AGCT 8 cut(s) 193, 244, 313, 461, 493, 574, 715, 943
AluI AGCT 8 cut(s) 193, 244, 313, 461, 493, 574, 715, 943
Alw21I GWGCWC 1 cut(s) 148
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 5 cut(s) 264, 331, 573, 671, 809
Ama87I CYCGRG 1 cut(s) 456
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 3 cut(s) 310, 562, 883
ArsI GACNNNNNNTTYG 4 cut(s) 479, 511, 660, 692
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 3 cut(s) 66, 894, 920
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 4 cut(s) 107, 223, 596, 627
AvaI CYCGRG 1 cut(s) 456
AvaII GGWCC 3 cut(s) 66, 894, 920
BalI TGGCCA 1 cut(s) 346
BbrPI CACGTG 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 814
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 322, 574, 870
BccI CCATC 4 cut(s) 122, 197, 741, 944
BceAI ACGGC 2 cut(s) 34, 86
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BciT130I CCWGG 3 cut(s) 70, 411, 651
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 3 cut(s) 278, 716, 961
BfmI CTRYAG 2 cut(s) 308, 925
BfrI CTTAAG 1 cut(s) 680
BfuAI ACCTGC 1 cut(s) 315
BglI GCCNNNNNGGC 1 cut(s) 119
BisI GCNGC 3 cut(s) 311, 563, 884
BlsI GCNGC 3 cut(s) 312, 564, 885
BmcAI AGTACT 1 cut(s) 787
Bme1390I CCNGG 4 cut(s) 70, 84, 411, 651
Bme18I GGWCC 3 cut(s) 66, 894, 920
BmeT110I CYCGRG 1 cut(s) 456
BmgT120I GGNCC 3 cut(s) 66, 894, 920
BmiI GGNNCC 4 cut(s) 40, 67, 895, 922
BmrFI CCNGG 4 cut(s) 70, 84, 411, 651
BmrI ACTGGG 1 cut(s) 650
BmsI GCATC 1 cut(s) 36
BmuI ACTGGG 1 cut(s) 650
BpiI GAAGAC 1 cut(s) 814
Bpu10I CCTNAGC 1 cut(s) 61
BpuEI CTTGAG 2 cut(s) 132, 717
BpuMI CCSGG 1 cut(s) 84
BsaAI YACGTR 1 cut(s) 518
BsaI GGTCTC 1 cut(s) 297
BsaJI CCNNGG 3 cut(s) 82, 409, 594
BsaWI WCCGGW 2 cut(s) 35, 265
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 88, 246
Bse1I ACTGG 3 cut(s) 347, 347, 645
Bse3DI GCAATG 2 cut(s) 166, 432
BseBI CCWGG 3 cut(s) 70, 411, 651
BseDI CCNNGG 3 cut(s) 82, 409, 594
BseGI GGATG 3 cut(s) 360, 514, 955
BseLI CCNNNNNNNGG 3 cut(s) 56, 88, 246
BseMI GCAATG 2 cut(s) 166, 432
BseMII CTCAG 2 cut(s) 712, 878
BseNI ACTGG 3 cut(s) 347, 347, 645
BseXI GCAGC 3 cut(s) 322, 574, 870
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 1 cut(s) 346
BsiHKAI GWGCWC 1 cut(s) 148
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 3 cut(s) 36, 84, 266
BslFI GGGAC 3 cut(s) 79, 765, 907
BslI CCNNNNNNNGG 3 cut(s) 56, 88, 246
BsmAI GTCTC 1 cut(s) 297
BsmFI GGGAC 3 cut(s) 79, 765, 907
BsnI GGCC 1 cut(s) 346
Bso31I GGTCTC 1 cut(s) 297
BsoBI CYCGRG 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 5 cut(s) 256, 336, 578, 663, 801
BspACI CCGC 2 cut(s) 111, 352
BspANI GGCC 1 cut(s) 346
BspCNI CTCAG 2 cut(s) 711, 879
BspFNI CGCG 1 cut(s) 219
BspHI TCATGA 1 cut(s) 907
BspLI GGNNCC 4 cut(s) 40, 67, 895, 922
BspMAI CTGCAG 1 cut(s) 312
BspMI ACCTGC 1 cut(s) 315
BspPI GGATC 5 cut(s) 264, 331, 573, 671, 809
BspQI GCTCTTC 2 cut(s) 747, 933
BspTI CTTAAG 1 cut(s) 680
BspTNI GGTCTC 1 cut(s) 297
BsrDI GCAATG 2 cut(s) 166, 432
BsrI ACTGG 3 cut(s) 347, 347, 645
BssECI CCNNGG 3 cut(s) 82, 409, 594
BssMI GATC 5 cut(s) 256, 336, 578, 663, 801
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 3 cut(s) 70, 411, 651
Bst4CI ACNGT 1 cut(s) 770
Bst6I CTCTTC 2 cut(s) 747, 933
BstAFI CTTAAG 1 cut(s) 680
BstBAI YACGTR 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 572, 876
BstDEI CTNAG 3 cut(s) 61, 698, 887
BstF5I GGATG 3 cut(s) 360, 514, 955
BstFNI CGCG 1 cut(s) 219
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 5 cut(s) 259, 339, 581, 666, 804
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 5 cut(s) 256, 336, 578, 663, 801
BstMWI GCNNNNNNNGC 4 cut(s) 119, 571, 737, 880
BstNI CCWGG 3 cut(s) 70, 411, 651
BstSCI CCNGG 4 cut(s) 68, 82, 409, 649
BstSFI CTRYAG 2 cut(s) 308, 925
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 3 cut(s) 322, 574, 870
BstV2I GAAGAC 1 cut(s) 814
BstXI CCANNNNNNTGG 1 cut(s) 417
BsuRI GGCC 1 cut(s) 346
BtgZI GCGATG 1 cut(s) 687
BtsCI GGATG 3 cut(s) 360, 514, 955
BtsIMutI CAGTG 4 cut(s) 340, 354, 638, 775
BveI ACCTGC 1 cut(s) 315
Cac8I GCNNGC 2 cut(s) 572, 876
CciI TCATGA 1 cut(s) 907
CfoI GCGC 1 cut(s) 219
Cfr13I GGNCC 3 cut(s) 66, 894, 920
CseI GACGC 1 cut(s) 691
Csp6I GTAC 4 cut(s) 252, 275, 414, 786
CviAII CATG 4 cut(s) 260, 558, 660, 908
CviQI GTAC 4 cut(s) 252, 275, 414, 786
DdeI CTNAG 3 cut(s) 61, 698, 887
DpnI GATC 5 cut(s) 258, 338, 580, 665, 803
DpnII GATC 5 cut(s) 256, 336, 578, 663, 801
DrdI GACNNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 344
Eam1104I CTCTTC 2 cut(s) 747, 933
EarI CTCTTC 2 cut(s) 747, 933
Eco130I CCWWGG 1 cut(s) 594
Eco31I GGTCTC 1 cut(s) 297
Eco32I GATATC 1 cut(s) 406
Eco47I GGWCC 3 cut(s) 66, 894, 920
Eco72I CACGTG 1 cut(s) 518
Eco88I CYCGRG 1 cut(s) 456
EcoO109I RGGNCCY 2 cut(s) 66, 894
EcoRII CCWGG 3 cut(s) 68, 409, 649
EcoRV GATATC 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 4 cut(s) 263, 561, 663, 911
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 3 cut(s) 79, 765, 907
FatI CATG 4 cut(s) 259, 557, 659, 907
Fnu4HI GCNGC 3 cut(s) 311, 563, 884
FokI GGATG 2 cut(s) 367, 521
Fsp4HI GCNGC 3 cut(s) 311, 563, 884
FspBI CTAG 3 cut(s) 278, 716, 961
GlaI GCGC 1 cut(s) 218
GluI GCNGC 3 cut(s) 311, 563, 884
HaeIII GGCC 1 cut(s) 346
HapII CCGG 3 cut(s) 36, 84, 266
HgaI GACGC 1 cut(s) 691
HhaI GCGC 1 cut(s) 219
Hin1II CATG 4 cut(s) 263, 561, 663, 911
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HinfI GANTC 4 cut(s) 443, 685, 691, 811
HpaII CCGG 3 cut(s) 36, 84, 266
HphI GGTGA 4 cut(s) 107, 223, 596, 627
Hpy166II GTNNAC 4 cut(s) 252, 535, 604, 956
Hpy188I TCNGA 3 cut(s) 499, 690, 865
Hpy188III TCNNGA 4 cut(s) 334, 815, 908, 935
Hpy8I GTNNAC 4 cut(s) 252, 535, 604, 956
Hpy99I CGWCG 2 cut(s) 107, 707
HpyAV CCTTC 2 cut(s) 355, 592
HpyCH4III ACNGT 1 cut(s) 770
HpyCH4IV ACGT 1 cut(s) 517
HpyCH4V TGCA 5 cut(s) 263, 310, 450, 588, 757
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 571, 737, 880
HpyF3I CTNAG 3 cut(s) 61, 698, 887
HpySE526I ACGT 1 cut(s) 517
Hsp92II CATG 4 cut(s) 263, 561, 663, 911
HspAI GCGC 1 cut(s) 217
Kzo9I GATC 5 cut(s) 256, 336, 578, 663, 801
LguI GCTCTTC 2 cut(s) 747, 933
LmnI GCTCC 1 cut(s) 38
Lsp1109I GCAGC 3 cut(s) 322, 574, 870
LweI GCATC 1 cut(s) 36
MaeI CTAG 3 cut(s) 278, 716, 961
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 1 cut(s) 764
MalI GATC 5 cut(s) 258, 338, 580, 665, 803
MboI GATC 5 cut(s) 256, 336, 578, 663, 801
MhlI GDGCHC 1 cut(s) 148
MlsI TGGCCA 1 cut(s) 346
MluCI AATT 4 cut(s) 224, 475, 845, 867
MluNI TGGCCA 1 cut(s) 346
MmeI TCCRAC 1 cut(s) 486
MnlI CCTC 7 cut(s) 180, 214, 247, 274, 534, 884, 907
Mox20I TGGCCA 1 cut(s) 346
MscI TGGCCA 1 cut(s) 346
MseI TTAA 2 cut(s) 378, 681
Msp20I TGGCCA 1 cut(s) 346
MspCI CTTAAG 1 cut(s) 680
MspI CCGG 3 cut(s) 36, 84, 266
MspR9I CCNGG 4 cut(s) 70, 84, 411, 651
MvaI CCWGG 3 cut(s) 70, 411, 651
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 4 cut(s) 119, 571, 737, 880
NciI CCSGG 1 cut(s) 84
NdeII GATC 5 cut(s) 256, 336, 578, 663, 801
NlaIII CATG 4 cut(s) 263, 561, 663, 911
NlaIV GGNNCC 4 cut(s) 40, 67, 895, 922
NmuCI GTSAC 1 cut(s) 764
PaeR7I CTCGAG 1 cut(s) 456
PagI TCATGA 1 cut(s) 907
PciSI GCTCTTC 2 cut(s) 747, 933
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PfeI GAWTC 4 cut(s) 443, 685, 691, 811
PflMI CCANNNNNTGG 1 cut(s) 246
PkrI GCNGC 3 cut(s) 312, 564, 885
PmaCI CACGTG 1 cut(s) 518
PmlI CACGTG 1 cut(s) 518
Ppu21I YACGTR 1 cut(s) 518
PpuMI RGGWCCY 2 cut(s) 66, 894
Psp5II RGGWCCY 2 cut(s) 66, 894
Psp6I CCWGG 3 cut(s) 68, 409, 649
PspCI CACGTG 1 cut(s) 518
PspGI CCWGG 3 cut(s) 68, 409, 649
PspN4I GGNNCC 4 cut(s) 40, 67, 895, 922
PspPI GGNCC 3 cut(s) 66, 894, 920
PspPPI RGGWCCY 2 cut(s) 66, 894
PspXI VCTCGAGB 1 cut(s) 456
PstI CTGCAG 1 cut(s) 312
RsaI GTAC 4 cut(s) 253, 276, 415, 787
RsaNI GTAC 4 cut(s) 252, 275, 414, 786
SapI GCTCTTC 2 cut(s) 747, 933
SaqAI TTAA 2 cut(s) 378, 681
SatI GCNGC 3 cut(s) 311, 563, 884
Sau3AI GATC 5 cut(s) 256, 336, 578, 663, 801
Sau96I GGNCC 3 cut(s) 66, 894, 920
ScaI AGTACT 1 cut(s) 787
ScrFI CCNGG 4 cut(s) 70, 84, 411, 651
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 1 cut(s) 36
SfcI CTRYAG 2 cut(s) 308, 925
Sfr274I CTCGAG 1 cut(s) 456
SinI GGWCC 3 cut(s) 66, 894, 920
SlaI CTCGAG 1 cut(s) 456
SmlI CTYRAG 4 cut(s) 147, 456, 680, 732
SmoI CTYRAG 4 cut(s) 147, 456, 680, 732
Sse9I AATT 4 cut(s) 224, 475, 845, 867
SsiI CCGC 2 cut(s) 111, 352
SspMI CTAG 3 cut(s) 278, 716, 961
StyD4I CCNGG 4 cut(s) 68, 82, 409, 649
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 1 cut(s) 770
TaiI ACGT 1 cut(s) 520
TaqI TCGA 2 cut(s) 457, 745
TasI AATT 4 cut(s) 224, 475, 845, 867
TatI WGTACW 2 cut(s) 274, 785
TfiI GAWTC 4 cut(s) 443, 685, 691, 811
Tru1I TTAA 2 cut(s) 378, 681
Tru9I TTAA 2 cut(s) 378, 681
TscAI CASTG 4 cut(s) 347, 354, 645, 775
TseFI GTSAC 1 cut(s) 764
TseI GCWGC 3 cut(s) 310, 562, 883
Tsp45I GTSAC 1 cut(s) 764
TspDTI ATGAA 3 cut(s) 371, 546, 896
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 4 cut(s) 347, 354, 645, 775
Van91I CCANNNNNTGG 1 cut(s) 246
Vha464I CTTAAG 1 cut(s) 680
VpaK11BI GGWCC 3 cut(s) 66, 894, 920
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XhoI CTCGAG 1 cut(s) 456
XspI CTAG 3 cut(s) 278, 716, 961
ZrmI AGTACT 1 cut(s) 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.