RLG00000024651

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001859
Physical Location & Seq
Forward (+)
37901655 .. 37907037
5383 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024651

Sequence Viewer

Length: 744 bp
ATGGCGGACGATATTGGTTTCTTTGAGTTGAACACCGGGGCTAAGATGCCTTCTTTGGGCTTAGGGACCTGGCAGGCCAGCCCTGGCTTGGTTGGTGACGCCGTCGCCGCCGCCATCAAGATCCACTGGCCAGTGCGGAAGAAGAAGGATTCAGTAGGTTTTGGGCCTGAAAACCTTATACAACCAGATATCCCTGCCACATGGAGAGCAATGGAAACTCTCTATGATTCATGCAAGGCTCGAGCTATTGGTGTGAGCAATTTCGCTACAAAGAAGCTGTCAGATTTGCTGGATGTGGCACGAGTTCGTCCTGCTGTTAACCAGGTGGAGTGCCATCCTTCGTGGCAGCAGGCCAAGCTGCGATCATTCTGCAAATCCAAGGGGGTTCACCTTTCTGGATATTCACCACTGGGTTCTCCTGGCACAACATTTATCAAAGGCGATGTCCTTAAGAATCCGATTCTTCTCAGCGTGGCAGAGAAGCTAGGCAAAACTCCTGCACAGGTTGCTCTTCGATGGGGACTGCAAATGGGTCATAGTGTTCTTCCAAAGAGTACTAATGAAGCAAGGATCAAGGAGAATCTCGATGTCTTTGGCTGGTCTATCCCAGAAGACTTGTTTGCCAAATTTTCTGAAATTGAGCAGGATAAACTTATCGAGATAGACTTGCTTACATGGGCAGAGTCAGAGTTTTGGGCTACTAGGATGTCTGGCAGAGAGATTGGCATAGGGCTAATAGCTTAG

Protein Analysis

248

Amino Acids

27.03

Weight (kDa)

8.37

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 40 - 213 5.9e-26 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 5, 108, 111, 136
AclWI GGATC 2 cut(s) 115, 578
AcoI YGGCCR 1 cut(s) 128
AcsI RAATTY 1 cut(s) 626
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 1 cut(s) 556
AfiI CCNNNNNNNGG 2 cut(s) 56, 88
AflII CTTAAG 1 cut(s) 449
AgsI TTSAA 1 cut(s) 31
AjnI CCWGG 4 cut(s) 68, 82, 321, 418
AluBI AGCT 5 cut(s) 245, 277, 358, 484, 740
AluI AGCT 5 cut(s) 245, 277, 358, 484, 740
AlwI GGATC 2 cut(s) 115, 578
Ama87I CYCGRG 1 cut(s) 240
AoxI GGCC 4 cut(s) 75, 128, 164, 351
ApeKI GCWGC 2 cut(s) 346, 358
ApoI RAATTY 1 cut(s) 626
ArsI GACNNNNNNTTYG 4 cut(s) 263, 295, 429, 461
AspS9I GGNCC 2 cut(s) 66, 164
AsuC2I CCSGG 1 cut(s) 37
AsuHPI GGTGA 3 cut(s) 107, 380, 396
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 1 cut(s) 66
BalI TGGCCA 1 cut(s) 130
BauI CACGAG 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 618
BbvI GCAGC 2 cut(s) 345, 358
BccI CCATC 3 cut(s) 122, 342, 510
BceAI ACGGC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 351, 385
BciT130I CCWGG 4 cut(s) 70, 84, 323, 420
BcnI CCSGG 1 cut(s) 37
BfaI CTAG 2 cut(s) 485, 702
BfrI CTTAAG 1 cut(s) 449
BisI GCNGC 4 cut(s) 108, 111, 347, 359
BlsI GCNGC 4 cut(s) 109, 112, 348, 360
BmcAI AGTACT 1 cut(s) 556
Bme1390I CCNGG 5 cut(s) 37, 70, 84, 323, 420
Bme18I GGWCC 1 cut(s) 66
BmeT110I CYCGRG 1 cut(s) 240
BmgT120I GGNCC 2 cut(s) 66, 164
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 5 cut(s) 37, 70, 84, 323, 420
BmrI ACTGGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 36
BmuI ACTGGG 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 618
Bpu10I CCTNAGC 1 cut(s) 61
BpuMI CCSGG 1 cut(s) 37
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 3 cut(s) 36, 82, 378
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 88
Bse1I ACTGG 3 cut(s) 131, 131, 414
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 4 cut(s) 70, 84, 323, 420
BseDI CCNNGG 3 cut(s) 36, 82, 378
BseGI GGATG 3 cut(s) 298, 334, 711
BseLI CCNNNNNNNGG 2 cut(s) 56, 88
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 1 cut(s) 481
BseNI ACTGG 3 cut(s) 131, 131, 414
BseXI GCAGC 2 cut(s) 345, 358
BsgI GTGCAG 1 cut(s) 483
BshFI GGCC 4 cut(s) 77, 130, 166, 353
BsiHKCI CYCGRG 1 cut(s) 240
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 2 cut(s) 79, 534
BslI CCNNNNNNNGG 2 cut(s) 56, 88
BsmFI GGGAC 2 cut(s) 79, 534
BsnI GGCC 4 cut(s) 77, 130, 166, 353
BsoBI CYCGRG 1 cut(s) 240
Bsp143I GATC 3 cut(s) 120, 362, 570
BspACI CCGC 4 cut(s) 5, 108, 111, 136
BspANI GGCC 4 cut(s) 77, 130, 166, 353
BspCNI CTCAG 1 cut(s) 480
BspLI GGNNCC 1 cut(s) 67
BspPI GGATC 2 cut(s) 115, 578
BspQI GCTCTTC 1 cut(s) 516
BspTI CTTAAG 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 216
BsrI ACTGG 3 cut(s) 131, 131, 414
BssECI CCNNGG 3 cut(s) 36, 82, 378
BssMI GATC 3 cut(s) 120, 362, 570
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 1 cut(s) 300
BssT1I CCWWGG 1 cut(s) 378
Bst2BI CACGAG 1 cut(s) 300
Bst2UI CCWGG 4 cut(s) 70, 84, 323, 420
Bst6I CTCTTC 1 cut(s) 516
BstACI GRCGYC 1 cut(s) 99
BstAFI CTTAAG 1 cut(s) 449
BstAPI GCANNNNNTGC 1 cut(s) 506
BstC8I GCNNGC 3 cut(s) 75, 79, 351
BstDEI CTNAG 4 cut(s) 42, 61, 467, 741
BstF5I GGATG 3 cut(s) 298, 334, 711
BstKTI GATC 3 cut(s) 123, 365, 573
BstMBI GATC 3 cut(s) 120, 362, 570
BstMWI GCNNNNNNNGC 3 cut(s) 107, 355, 506
BstNI CCWGG 4 cut(s) 70, 84, 323, 420
BstSCI CCNGG 5 cut(s) 35, 68, 82, 321, 418
BstV1I GCAGC 2 cut(s) 345, 358
BstV2I GAAGAC 1 cut(s) 618
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BsuRI GGCC 4 cut(s) 77, 130, 166, 353
BtgZI GCGATG 1 cut(s) 456
BtsCI GGATG 3 cut(s) 298, 334, 711
BtsIMutI CAGTG 3 cut(s) 124, 138, 407
Cac8I GCNNGC 3 cut(s) 75, 79, 351
Cfr13I GGNCC 2 cut(s) 66, 164
CseI GACGC 1 cut(s) 107
CsiI ACCWGGT 1 cut(s) 321
Csp6I GTAC 1 cut(s) 555
CviAII CATG 3 cut(s) 201, 231, 675
CviQI GTAC 1 cut(s) 555
DdeI CTNAG 4 cut(s) 42, 61, 467, 741
DpnI GATC 3 cut(s) 122, 364, 572
DpnII GATC 3 cut(s) 120, 362, 570
EaeI YGGCCR 1 cut(s) 128
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 378
Eco32I GATATC 1 cut(s) 190
Eco47I GGWCC 1 cut(s) 66
Eco88I CYCGRG 1 cut(s) 240
EcoO109I RGGNCCY 1 cut(s) 66
EcoRII CCWGG 4 cut(s) 68, 82, 321, 418
EcoRV GATATC 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 3 cut(s) 204, 234, 678
FaiI YATR 7 cut(s) 179, 202, 225, 232, 537, 676, 728
FaqI GGGAC 2 cut(s) 79, 534
FatI CATG 3 cut(s) 200, 230, 674
Fnu4HI GCNGC 4 cut(s) 108, 111, 347, 359
FokI GGATG 3 cut(s) 305, 321, 718
Fsp4HI GCNGC 4 cut(s) 108, 111, 347, 359
FspBI CTAG 2 cut(s) 485, 702
GluI GCNGC 4 cut(s) 108, 111, 347, 359
HaeIII GGCC 4 cut(s) 77, 130, 166, 353
HapII CCGG 1 cut(s) 36
HgaI GACGC 1 cut(s) 107
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 3 cut(s) 204, 234, 678
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 6 cut(s) 149, 227, 454, 460, 580, 683
HpaI GTTAAC 1 cut(s) 319
HpaII CCGG 1 cut(s) 36
HphI GGTGA 3 cut(s) 107, 380, 396
Hpy166II GTNNAC 2 cut(s) 319, 388
Hpy188I TCNGA 4 cut(s) 283, 459, 634, 688
Hpy188III TCNNGA 4 cut(s) 118, 396, 584, 658
Hpy8I GTNNAC 2 cut(s) 319, 388
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 3 cut(s) 60, 139, 348
HpyCH4V TGCA 4 cut(s) 234, 372, 500, 526
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 355, 506
HpyF3I CTNAG 4 cut(s) 42, 61, 467, 741
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 3 cut(s) 204, 234, 678
KspAI GTTAAC 1 cut(s) 319
Kzo9I GATC 3 cut(s) 120, 362, 570
LguI GCTCTTC 1 cut(s) 516
Lsp1109I GCAGC 2 cut(s) 345, 358
LweI GCATC 1 cut(s) 36
MabI ACCWGGT 1 cut(s) 321
MaeI CTAG 2 cut(s) 485, 702
MaeIII GTNAC 1 cut(s) 95
MalI GATC 3 cut(s) 122, 364, 572
MboI GATC 3 cut(s) 120, 362, 570
MboII GAAGA 6 cut(s) 151, 154, 455, 503, 536, 623
MflI RGATCY 1 cut(s) 120
MlsI TGGCCA 1 cut(s) 130
MluCI AATT 3 cut(s) 259, 626, 636
MluNI TGGCCA 1 cut(s) 130
MlyI GAGTC 1 cut(s) 692
Mox20I TGGCCA 1 cut(s) 130
MscI TGGCCA 1 cut(s) 130
MseI TTAA 2 cut(s) 318, 450
Msp20I TGGCCA 1 cut(s) 130
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 5 cut(s) 37, 70, 84, 323, 420
MvaI CCWGG 4 cut(s) 70, 84, 323, 420
MwoI GCNNNNNNNGC 3 cut(s) 107, 355, 506
NciI CCSGG 1 cut(s) 37
NdeII GATC 3 cut(s) 120, 362, 570
NlaIII CATG 3 cut(s) 204, 234, 678
NlaIV GGNNCC 1 cut(s) 67
NmuCI GTSAC 1 cut(s) 95
PaeR7I CTCGAG 1 cut(s) 240
PciSI GCTCTTC 1 cut(s) 516
PfeI GAWTC 5 cut(s) 149, 227, 454, 460, 580
PflFI GACNNNGTC 1 cut(s) 101
PkrI GCNGC 4 cut(s) 109, 112, 348, 360
PleI GAGTC 1 cut(s) 691
PpsI GAGTC 1 cut(s) 691
PpuMI RGGWCCY 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 66
Psp6I CCWGG 4 cut(s) 68, 82, 321, 418
PspGI CCWGG 4 cut(s) 68, 82, 321, 418
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 2 cut(s) 66, 164
PspPPI RGGWCCY 1 cut(s) 66
PspXI VCTCGAGB 1 cut(s) 240
PsuI RGATCY 1 cut(s) 120
PsyI GACNNNGTC 1 cut(s) 101
RsaI GTAC 1 cut(s) 556
RsaNI GTAC 1 cut(s) 555
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 2 cut(s) 318, 450
SatI GCNGC 4 cut(s) 108, 111, 347, 359
Sau3AI GATC 3 cut(s) 120, 362, 570
Sau96I GGNCC 2 cut(s) 66, 164
ScaI AGTACT 1 cut(s) 556
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 5 cut(s) 37, 70, 84, 323, 420
SexAI ACCWGGT 1 cut(s) 321
SfaNI GCATC 1 cut(s) 36
Sfr274I CTCGAG 1 cut(s) 240
SinI GGWCC 1 cut(s) 66
SlaI CTCGAG 1 cut(s) 240
SmlI CTYRAG 2 cut(s) 240, 449
SmoI CTYRAG 2 cut(s) 240, 449
Sse9I AATT 3 cut(s) 259, 626, 636
SsiI CCGC 4 cut(s) 5, 108, 111, 136
SspMI CTAG 2 cut(s) 485, 702
StyD4I CCNGG 5 cut(s) 35, 68, 82, 321, 418
StyI CCWWGG 1 cut(s) 378
TaqI TCGA 4 cut(s) 241, 514, 585, 657
TasI AATT 3 cut(s) 259, 626, 636
TatI WGTACW 1 cut(s) 554
TauI GCSGC 2 cut(s) 110, 113
TfiI GAWTC 5 cut(s) 149, 227, 454, 460, 580
Tru1I TTAA 2 cut(s) 318, 450
Tru9I TTAA 2 cut(s) 318, 450
TscAI CASTG 3 cut(s) 131, 138, 414
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 2 cut(s) 346, 358
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 2 cut(s) 219, 576
TspRI CASTG 3 cut(s) 131, 138, 414
Tth111I GACNNNGTC 1 cut(s) 101
Vha464I CTTAAG 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 66
XapI RAATTY 1 cut(s) 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XhoI CTCGAG 1 cut(s) 240
XspI CTAG 2 cut(s) 485, 702
ZrmI AGTACT 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.