Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001859

Physical Location & Seq

Genomic Coordinates Forward (+)

23049252 .. 23051659

Gene Structure 2408 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1150200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 948 bp

ATGGCGGACGACATTCTCTTCTTCGAGCTGAACACCGGCGCTAAGATCCCGTCGATAGGGTTGGGGACCTGGCAGGCAAGCCCTGGCCTTGTCGGCGAATCGGTCCACGCCGCCATCAAGGCAGGGTATCGGCATATTGATTGTGCTCAAATCTACGGCAATGAGAAGGAGATTGGTTTGGTGCTGAAGAAACTGTTTGAAGATGGTGTGGTGAAGCGCGAGGATTTGTGGATTACCTCCAAACTCTGGAATACTGATCATGCTCCAGAAGATGTACCAGAGGCATTGGACAGGACTCTGAGAGACTTGCAACTGGATTATGTTGACTTGTACCTTATGCACTGGCCAGTCCGGATGAAGAAGGGATCAGTAGGTTTTAAGCCTGAAAATATTATTGACCCTGATGTCCCTGCAACTTGGAGAGCAATGGAAACACTCTATGATTCGGGAAAGGCTCGAGCTATTGGTGTGAGCAATTTCTCCACAAAAAAGCTGTCAGATTTGCTGGATGTCGCACGTGTTCCTCCTGCTGTTGACCAAGTGGAGTGCCATCCCTCGTGGCAGCAGGACAAGCTACGTTCTTTCTGTAAATCCAAGGGAGTTCACCTTTCTGGATATTCCCCACTGGGCTCTCCTGGAACAACATGGATCAAAAGTGAAGTCCTTAAGAATCCGATTCTTGTGACAGTCGCTGAGAAATTAGGCAAAACTCCTGCACAAGTTGCTCTCCGTTGGGGACTGCAAAAGGGTCATAGTGTTCTTCCCAAGAGTACCAATGAAGCGAGGATCAAGGAGAACCTTGATGTGTTTGGCTGGTCTATCCCAGATGACTTGTTCGCCAAGTTTTCTGAAATTGAGCAGGCAAGACTGATCAGAGGGGCCACATTTGTTCATGACACTTTTGGTCCCTACAGGAGTTTAGAGGAATTCTGGGACGGTGAAATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001101 GO:0003674 GO:0003824 GO:0004033 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006970 GO:0008106 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0010035 GO:0016229 GO:0016491 GO:0016614 GO:0016616 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048037 GO:0050661 GO:0050662 GO:0050896 GO:0055114 GO:0070401 GO:0097159 GO:1901265 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00010 ko00040 ko00561 ko00930 ko01100 ko01110 ko01120 ko01130 ko01220 map00010 map00040 map00561 map00930 map01100 map01110 map01120 map01130 map01220

Pfam Domains

Protein Families

Aldo_ket_red

Protein Analysis

316

Amino Acids

35.24

Weight (kDa)

5.94

Isoelectric Point (pI)

39.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aldo_ket_red	PF00248	19 - 285	3e-54	Aldo/keto reductase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001859)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37770
fragaria_vesca	FvH4_6g13020 FvH4_6g13020 FvH4_6g13020 FvH4_6g13020 FvH4_6g13020 FvH4_6g13020 FvH4_6g13020 FvH4_6g13020
malus_domestica	MD12G1150200.v1.1
prunus_persica	Prupe.6G094300_v2.0.a1 Prupe.6G261900_v2.0.a1
pyrus_communis	pycom04g12320 pycom12g14390 pycom12g14400
rosa_chinensis	RchiOBHm_Chr3g0465151 RchiOBHm_Chr3g0465231 RchiOBHm_Chr3g0465271 RchiOBHm_Chr3g0465311
rosa_laevigata	RLG00000024648 RLG00000024651 RLG00000024653
rosa_multiflora	Rmu_co8067008.1_g000001 Rmu_co8210356.1_g000001 Rmu_sc0003386.1_g000008 Rmu_sc0003386.1_g000010 Rmu_sc0003492.1_g000019 Rmu_sc0018026.1_g000002 Rmu_sc0021566.1_g000001
rosa_roxburghii	Rroxscaffold_6G00415190 Rroxscaffold_6G00415200
rosa_rugosa	Rorug03G0075800 Rorug03G0075900 Rorug03G0076000 Rorug03G0076100 Rorug03G0076200 Rorug03G0076200
rosa_samantha	Rh3BG139500 Rh3BG140100 Rh3BG140500 Rh3DG140800 Rh3DG141600
rosa_wichuraiana	Rw3G011420 Rw3G011430 Rw3G011470 Rw3G011490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	404
AccB7I	CCANNNNNTGG	1 cut(s)	246
AccII	CGCG	1 cut(s)	219
AccIII	TCCGGA	1 cut(s)	351
AciI	CCGC	2 cut(s)	5, 111
AclWI	GGATC	4 cut(s)	40, 373, 656, 794
AcoI	YGGCCR	1 cut(s)	344
AcsI	RAATTY	1 cut(s)	926
AcuI	CTGAAG	1 cut(s)	206
AcvI	CACGTG	1 cut(s)	518
AfaI	GTAC	3 cut(s)	276, 332, 772
AfiI	CCNNNNNNNGG	2 cut(s)	56, 246
AflII	CTTAAG	1 cut(s)	665
AflIII	ACRYGT	1 cut(s)	517
AgsI	TTSAA	1 cut(s)	200
AjnI	CCWGG	3 cut(s)	68, 82, 634
AluBI	AGCT	4 cut(s)	28, 461, 493, 574
AluI	AGCT	4 cut(s)	28, 461, 493, 574
Alw21I	GWGCWC	1 cut(s)	148
Alw26I	GTCTC	1 cut(s)	297
AlwI	GGATC	4 cut(s)	40, 373, 656, 794
AlwNI	CAGNNNCTG	1 cut(s)	692
Ama87I	CYCGRG	1 cut(s)	456
Aor13HI	TCCGGA	1 cut(s)	351
AoxI	GGCC	3 cut(s)	85, 344, 879
ApeKI	GCWGC	1 cut(s)	562
ApoI	RAATTY	1 cut(s)	926
ArsI	GACNNNNNNTTYG	4 cut(s)	479, 511, 645, 677
AspLEI	GCGC	2 cut(s)	41, 219
AspS9I	GGNCC	4 cut(s)	66, 103, 879, 905
AsuHPI	GGTGA	2 cut(s)	223, 596
AvaI	CYCGRG	1 cut(s)	456
AvaII	GGWCC	3 cut(s)	66, 103, 905
BalI	TGGCCA	1 cut(s)	346
BanII	GRGCYC	1 cut(s)	632
BauI	CACGAG	1 cut(s)	556
BbrPI	CACGTG	1 cut(s)	518
Bbv12I	GWGCWC	1 cut(s)	148
BbvI	GCAGC	1 cut(s)	574
BccI	CCATC	3 cut(s)	122, 197, 558
BceAI	ACGGC	1 cut(s)	172
BciT130I	CCWGG	3 cut(s)	70, 84, 636
BclI	TGATCA	2 cut(s)	256, 870
BcoDI	GTCTC	1 cut(s)	297
BfaI	CTAG	1 cut(s)	946
BfmI	CTRYAG	1 cut(s)	910
BfoI	RGCGCY	1 cut(s)	42
BfrI	CTTAAG	1 cut(s)	665
BglI	GCCNNNNNGGC	2 cut(s)	93, 119
BisI	GCNGC	2 cut(s)	111, 563
BlsI	GCNGC	2 cut(s)	112, 564
Bme1390I	CCNGG	3 cut(s)	70, 84, 636
Bme18I	GGWCC	3 cut(s)	66, 103, 905
BmeT110I	CYCGRG	1 cut(s)	456
BmgT120I	GGNCC	4 cut(s)	66, 103, 879, 905
BmiI	GGNNCC	3 cut(s)	67, 880, 907
BmrFI	CCNGG	3 cut(s)	70, 84, 636
BmrI	ACTGGG	1 cut(s)	635
BmuI	ACTGGG	1 cut(s)	635
BpmI	CTGGAG	1 cut(s)	249
BsaAI	YACGTR	1 cut(s)	518
BsaJI	CCNNGG	2 cut(s)	82, 594
BsaWI	WCCGGW	1 cut(s)	351
Bsc4I	CCNNNNNNNGG	2 cut(s)	56, 246
Bse118I	RCCGGY	1 cut(s)	35
Bse1I	ACTGG	4 cut(s)	318, 347, 347, 630
Bse3DI	GCAATG	2 cut(s)	166, 432
BseAI	TCCGGA	1 cut(s)	351
BseBI	CCWGG	3 cut(s)	70, 84, 636
BseDI	CCNNGG	2 cut(s)	82, 594
BseGI	GGATG	3 cut(s)	360, 514, 550
BseLI	CCNNNNNNNGG	2 cut(s)	56, 246
BseMI	GCAATG	2 cut(s)	166, 432
BseMII	CTCAG	2 cut(s)	290, 684
BseNI	ACTGG	4 cut(s)	318, 347, 347, 630
BseXI	GCAGC	1 cut(s)	574
BsgI	GTGCAG	1 cut(s)	699
Bsh1236I	CGCG	1 cut(s)	219
BshFI	GGCC	3 cut(s)	87, 346, 881
BsiHKAI	GWGCWC	1 cut(s)	148
BsiHKCI	CYCGRG	1 cut(s)	456
BsiSI	CCGG	2 cut(s)	36, 352
BslFI	GGGAC	4 cut(s)	79, 392, 750, 891
BslI	CCNNNNNNNGG	2 cut(s)	56, 246
BsmAI	GTCTC	1 cut(s)	297
BsmFI	GGGAC	4 cut(s)	79, 392, 750, 891
BsnI	GGCC	3 cut(s)	87, 346, 881
BsoBI	CYCGRG	1 cut(s)	456
Bsp1286I	GDGCHC	2 cut(s)	148, 632
Bsp13I	TCCGGA	1 cut(s)	351
Bsp143I	GATC	6 cut(s)	45, 256, 365, 648, 786, 870
BspACI	CCGC	2 cut(s)	5, 111
BspANI	GGCC	3 cut(s)	87, 346, 881
BspCNI	CTCAG	2 cut(s)	291, 685
BspEI	TCCGGA	1 cut(s)	351
BspFNI	CGCG	1 cut(s)	219
BspHI	TCATGA	1 cut(s)	892
BspLI	GGNNCC	3 cut(s)	67, 880, 907
BspPI	GGATC	4 cut(s)	40, 373, 656, 794
BspTI	CTTAAG	1 cut(s)	665
BsrDI	GCAATG	2 cut(s)	166, 432
BsrFI	RCCGGY	1 cut(s)	35
BsrI	ACTGG	4 cut(s)	318, 347, 347, 630
BssAI	RCCGGY	1 cut(s)	35
BssECI	CCNNGG	2 cut(s)	82, 594
BssMI	GATC	6 cut(s)	45, 256, 365, 648, 786, 870
BssSI	CACGAG	1 cut(s)	556
BssT1I	CCWWGG	1 cut(s)	594
Bst2BI	CACGAG	1 cut(s)	556
Bst2UI	CCWGG	3 cut(s)	70, 84, 636
Bst4CI	ACNGT	3 cut(s)	195, 688, 938
Bst6I	CTCTTC	1 cut(s)	23
BstAFI	CTTAAG	1 cut(s)	665
BstAPI	GCANNNNNTGC	1 cut(s)	722
BstBAI	YACGTR	1 cut(s)	518
BstC8I	GCNNGC	3 cut(s)	75, 79, 861
BstDEI	CTNAG	3 cut(s)	42, 299, 693
BstF5I	GGATG	3 cut(s)	360, 514, 550
BstFNI	CGCG	1 cut(s)	219
BstH2I	RGCGCY	1 cut(s)	42
BstHHI	GCGC	2 cut(s)	41, 219
BstKTI	GATC	6 cut(s)	48, 259, 368, 651, 789, 873
BstMAI	GTCTC	1 cut(s)	297
BstMBI	GATC	6 cut(s)	45, 256, 365, 648, 786, 870
BstMWI	GCNNNNNNNGC	4 cut(s)	93, 119, 571, 722
BstNI	CCWGG	3 cut(s)	70, 84, 636
BstSCI	CCNGG	3 cut(s)	68, 82, 634
BstSFI	CTRYAG	1 cut(s)	910
BstUI	CGCG	1 cut(s)	219
BstV1I	GCAGC	1 cut(s)	574
BstX2I	RGATCY	1 cut(s)	45
BstYI	RGATCY	1 cut(s)	45
BsuRI	GGCC	3 cut(s)	87, 346, 881
BtsCI	GGATG	3 cut(s)	360, 514, 550
BtsIMutI	CAGTG	2 cut(s)	340, 623
Cac8I	GCNNGC	3 cut(s)	75, 79, 861
CaiI	CAGNNNCTG	1 cut(s)	692
CciI	TCATGA	1 cut(s)	892
CfoI	GCGC	2 cut(s)	41, 219
Cfr10I	RCCGGY	1 cut(s)	35
Cfr13I	GGNCC	4 cut(s)	66, 103, 879, 905
Csp6I	GTAC	3 cut(s)	275, 331, 771
CviAII	CATG	3 cut(s)	260, 645, 893
CviQI	GTAC	3 cut(s)	275, 331, 771
DdeI	CTNAG	3 cut(s)	42, 299, 693
DpnI	GATC	6 cut(s)	47, 258, 367, 650, 788, 872
DpnII	GATC	6 cut(s)	45, 256, 365, 648, 786, 870
DrdI	GACNNNNNNGTC	1 cut(s)	404
DseDI	GACNNNNNNGTC	1 cut(s)	404
EaeI	YGGCCR	1 cut(s)	344
Eam1104I	CTCTTC	1 cut(s)	23
EarI	CTCTTC	1 cut(s)	23
EciI	GGCGGA	1 cut(s)	20
Eco130I	CCWWGG	1 cut(s)	594
Eco24I	GRGCYC	1 cut(s)	632
Eco47I	GGWCC	3 cut(s)	66, 103, 905
Eco57I	CTGAAG	1 cut(s)	206
Eco72I	CACGTG	1 cut(s)	518
Eco88I	CYCGRG	1 cut(s)	456
EcoO109I	RGGNCCY	1 cut(s)	66
EcoRI	GAATTC	1 cut(s)	926
EcoRII	CCWGG	3 cut(s)	68, 82, 634
EcoT14I	CCWWGG	1 cut(s)	594
EcoT38I	GRGCYC	1 cut(s)	632
ErhI	CCWWGG	1 cut(s)	594
FaeI	CATG	3 cut(s)	263, 648, 896
FaiI	YATR	8 cut(s)	135, 261, 321, 338, 441, 646, 753, 894
FaqI	GGGAC	4 cut(s)	79, 392, 750, 891
FatI	CATG	3 cut(s)	259, 644, 892
FbaI	TGATCA	2 cut(s)	256, 870
Fnu4HI	GCNGC	2 cut(s)	111, 563
FokI	GGATG	3 cut(s)	367, 521, 537
FriOI	GRGCYC	1 cut(s)	632
Fsp4HI	GCNGC	2 cut(s)	111, 563
FspBI	CTAG	1 cut(s)	946
GlaI	GCGC	2 cut(s)	40, 218
GluI	GCNGC	2 cut(s)	111, 563
GsuI	CTGGAG	1 cut(s)	249
HaeII	RGCGCY	1 cut(s)	42
HaeIII	GGCC	3 cut(s)	87, 346, 881
HapII	CCGG	2 cut(s)	36, 352
HhaI	GCGC	2 cut(s)	41, 219
Hin1II	CATG	3 cut(s)	263, 648, 896
Hin6I	GCGC	2 cut(s)	39, 217
HinP1I	GCGC	2 cut(s)	39, 217
HincII	GTYRAC	2 cut(s)	325, 535
HindII	GTYRAC	2 cut(s)	325, 535
HinfI	GANTC	5 cut(s)	98, 295, 443, 670, 676
HpaII	CCGG	2 cut(s)	36, 352
HphI	GGTGA	2 cut(s)	223, 596
Hpy166II	GTNNAC	4 cut(s)	106, 325, 535, 604
Hpy188I	TCNGA	5 cut(s)	300, 499, 675, 850, 875
Hpy188III	TCNNGA	6 cut(s)	247, 266, 352, 447, 612, 893
Hpy8I	GTNNAC	4 cut(s)	106, 325, 535, 604
Hpy99I	CGWCG	1 cut(s)	55
HpyAV	CCTTC	2 cut(s)	160, 355
HpyCH4III	ACNGT	3 cut(s)	195, 688, 938
HpyCH4IV	ACGT	2 cut(s)	517, 577
HpyCH4V	TGCA	5 cut(s)	310, 340, 413, 716, 742
HpyF10VI	GCNNNNNNNGC	4 cut(s)	93, 119, 571, 722
HpyF3I	CTNAG	3 cut(s)	42, 299, 693
HpySE526I	ACGT	2 cut(s)	517, 577
Hsp92II	CATG	3 cut(s)	263, 648, 896
HspAI	GCGC	2 cut(s)	39, 217
Kpn2I	TCCGGA	1 cut(s)	351
Ksp22I	TGATCA	2 cut(s)	256, 870
Kzo9I	GATC	6 cut(s)	45, 256, 365, 648, 786, 870
LmnI	GCTCC	1 cut(s)	268
Lsp1109I	GCAGC	1 cut(s)	574
MaeI	CTAG	1 cut(s)	946
MaeII	ACGT	2 cut(s)	517, 577
MaeIII	GTNAC	1 cut(s)	682
MalI	GATC	6 cut(s)	47, 258, 367, 650, 788, 872
MboI	GATC	6 cut(s)	45, 256, 365, 648, 786, 870
MboII	GAAGA	7 cut(s)	10, 13, 199, 212, 281, 370, 752
MflI	RGATCY	1 cut(s)	45
MhlI	GDGCHC	2 cut(s)	148, 632
MlsI	TGGCCA	1 cut(s)	346
MluCI	AATT	4 cut(s)	475, 698, 852, 926
MluNI	TGGCCA	1 cut(s)	346
MlyI	GAGTC	1 cut(s)	289
MnlI	CCTC	8 cut(s)	214, 247, 274, 534, 565, 777, 869, 916
Mox20I	TGGCCA	1 cut(s)	346
MroI	TCCGGA	1 cut(s)	351
MscI	TGGCCA	1 cut(s)	346
MseI	TTAA	2 cut(s)	378, 666
Msp20I	TGGCCA	1 cut(s)	346
MspCI	CTTAAG	1 cut(s)	665
MspI	CCGG	2 cut(s)	36, 352
MspR9I	CCNGG	3 cut(s)	70, 84, 636
MvaI	CCWGG	3 cut(s)	70, 84, 636
MvnI	CGCG	1 cut(s)	219
MwoI	GCNNNNNNNGC	4 cut(s)	93, 119, 571, 722
NdeII	GATC	6 cut(s)	45, 256, 365, 648, 786, 870
NlaIII	CATG	3 cut(s)	263, 648, 896
NlaIV	GGNNCC	3 cut(s)	67, 880, 907
NmuCI	GTSAC	1 cut(s)	682
PaeR7I	CTCGAG	1 cut(s)	456
PagI	TCATGA	1 cut(s)	892
PfeI	GAWTC	4 cut(s)	98, 443, 670, 676
PflMI	CCANNNNNTGG	1 cut(s)	246
PfoI	TCCNGGA	1 cut(s)	634
PkrI	GCNGC	2 cut(s)	112, 564
PleI	GAGTC	1 cut(s)	289
PmaCI	CACGTG	1 cut(s)	518
PmlI	CACGTG	1 cut(s)	518
PpsI	GAGTC	1 cut(s)	289
Ppu21I	YACGTR	1 cut(s)	518
PpuMI	RGGWCCY	1 cut(s)	66
Psp5II	RGGWCCY	1 cut(s)	66
Psp6I	CCWGG	3 cut(s)	68, 82, 634
PspCI	CACGTG	1 cut(s)	518
PspGI	CCWGG	3 cut(s)	68, 82, 634
PspN4I	GGNNCC	3 cut(s)	67, 880, 907
PspPI	GGNCC	4 cut(s)	66, 103, 879, 905
PspPPI	RGGWCCY	1 cut(s)	66
PspXI	VCTCGAGB	1 cut(s)	456
PstNI	CAGNNNCTG	1 cut(s)	692
PsuI	RGATCY	1 cut(s)	45
RsaI	GTAC	3 cut(s)	276, 332, 772
RsaNI	GTAC	3 cut(s)	275, 331, 771
SaqAI	TTAA	2 cut(s)	378, 666
SatI	GCNGC	2 cut(s)	111, 563
Sau3AI	GATC	6 cut(s)	45, 256, 365, 648, 786, 870
Sau96I	GGNCC	4 cut(s)	66, 103, 879, 905
SchI	GAGTC	1 cut(s)	289
ScrFI	CCNGG	3 cut(s)	70, 84, 636
SduI	GDGCHC	2 cut(s)	148, 632
SfcI	CTRYAG	1 cut(s)	910
Sfr274I	CTCGAG	1 cut(s)	456
SgrAI	CRCCGGYG	1 cut(s)	35
SinI	GGWCC	3 cut(s)	66, 103, 905
SlaI	CTCGAG	1 cut(s)	456
SmlI	CTYRAG	2 cut(s)	456, 665
SmoI	CTYRAG	2 cut(s)	456, 665
Sse9I	AATT	4 cut(s)	475, 698, 852, 926
SsiI	CCGC	2 cut(s)	5, 111
SspI	AATATT	1 cut(s)	391
SspMI	CTAG	1 cut(s)	946
StyD4I	CCNGG	3 cut(s)	68, 82, 634
StyI	CCWWGG	1 cut(s)	594
TaaI	ACNGT	3 cut(s)	195, 688, 938
TaiI	ACGT	2 cut(s)	520, 580
TaqI	TCGA	3 cut(s)	24, 53, 457
TaqII	GACCGA	1 cut(s)	91
TasI	AATT	4 cut(s)	475, 698, 852, 926
TauI	GCSGC	1 cut(s)	113
TfiI	GAWTC	4 cut(s)	98, 443, 670, 676
Tru1I	TTAA	2 cut(s)	378, 666
Tru9I	TTAA	2 cut(s)	378, 666
TscAI	CASTG	2 cut(s)	347, 630
TseFI	GTSAC	1 cut(s)	682
TseI	GCWGC	1 cut(s)	562
Tsp45I	GTSAC	1 cut(s)	682
TspDTI	ATGAA	3 cut(s)	371, 792, 881
TspGWI	ACGGA	1 cut(s)	719
TspRI	CASTG	2 cut(s)	347, 630
Van91I	CCANNNNNTGG	1 cut(s)	246
Vha464I	CTTAAG	1 cut(s)	665
VpaK11BI	GGWCC	3 cut(s)	66, 103, 905
XapI	RAATTY	1 cut(s)	926
XhoI	CTCGAG	1 cut(s)	456
XspI	CTAG	1 cut(s)	946

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.